Porcine epidemic diarrhea virus g2b subtype strain and application thereof

By isolating and purifying the porcine epidemic diarrhea virus G2b subtype strain GZ2504, an inactivated vaccine and ELISA kit were prepared, solving the problem of low virus titer, achieving efficient virus control and diagnosis, and reducing economic losses.

CN122104607APending Publication Date: 2026-05-29CHINA ANIMAL HUSBANDRY IND
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA ANIMAL HUSBANDRY IND
Filing Date
2026-02-10
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies are insufficient for effectively isolating and culturing porcine epidemic diarrhea virus G2b subtype strains, resulting in low viral titers and a lack of highly effective vaccines and diagnostic reagents, leading to economic losses for the livestock industry.

Method used

A porcine epidemic diarrhea virus (PEDV) G2b subtype strain, GZ2504, was isolated and purified, and a corresponding inactivated vaccine and ELISA kit were prepared. Utilizing the high proliferation rate and titer of this strain on Vero cells, the prepared vaccine was able to induce high levels of neutralizing antibodies, and the ELISA kit could detect the antibodies with sensitivity and specificity.

Benefits of technology

It provides an efficient method for virus propagation, the inactivated vaccine prepared has good immunogenicity, and the ELISA kit has high sensitivity and specificity, supporting effective virus control and diagnosis and reducing economic losses.

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Abstract

The application discloses a porcine epidemic diarrhea virus G2b subtype strain and application thereof. The application obtains a porcine epidemic diarrhea virus G2b subtype GZ2504 strain from a porcine intestinal tissue, and the strain is purified through passage, and the microorganism preservation number of the strain is CGMCC No. 47083. The isolated strain can stably proliferate on a passage cell and produce a typical cytopathic effect. The porcine epidemic diarrhea virus G2b subtype isolated strain has excellent immunogenicity; a vaccine prepared by using the isolated strain can induce piglets to produce high-level neutralizing antibodies; meanwhile, an ELISA kit prepared by using the isolated strain can better detect porcine epidemic diarrhea virus antibodies in serum.
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