High-stability immobilized mannase as well as preparation method and application thereof

By immobilizing mannanase using PAMAM-modified SBA-15/calcium alginate composite microspheres and an encapsulation-covalent cross-linking method, the problems of weak enzyme-carrier binding and enzyme leakage were solved, achieving high stability and efficient immobilization, which is suitable for applications in multiple fields.

CN122104670APending Publication Date: 2026-05-29HUNAN LONGSEN BIOLOGICAL TECH CO LTD
0 Cites 0 Cited by

Patent Information

Application Number
CN202610390782.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-27
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing mannanase immobilization methods suffer from weak enzyme-carrier binding, uneven distribution of active sites on the carrier surface, and enzyme leakage, resulting in insufficient stability and immobilization efficiency, which limits their large-scale industrial application.

Method used

PAMAM-modified SBA-15/calcium alginate composite microspheres were used as carriers, and mannanase was immobilized by encapsulation-covalent cross-linking method. PAMAM provided abundant active sites and calcium alginate provided network support, so as to achieve stable encapsulation and covalent binding of enzyme.

Benefits of technology

It significantly improves the immobilization efficiency and stability of mannanase, enhances its tolerance to high temperatures and pH changes, and is suitable for applications in feed, food, and biomass degradation.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses high-stability immobilized mannase and a preparation method and application thereof, and belongs to the technical field of enzyme immobilization, according to the preparation method, PAMAM modified SBA-15 / calcium alginate composite microspheres serve as a carrier, mannase in enzyme liquid is immobilized through an embedding-covalent cross-linking method, and the high-stability immobilized mannase is obtained. According to the preparation method disclosed by the invention, PAMAM modified SBA-15 / calcium alginate composite microspheres are taken as a carrier, mannanase is immobilized by adopting an embedding-covalent cross-linking synergistic method, so that the pH tolerance, the cycling stability and the blood glucose reducing efficiency of the enzyme are improved, and the problems that the traditional immobilized enzyme is large in enzyme activity loss, easy to leak and poor in stability at high temperature are solved.
Need to check novelty before this filing date? Find Prior Art