Gene knu for controlling nucellar male sterility of navel orange and application thereof

By identifying and utilizing the anther-specific gene KNU, a Cas9 editing mode was designed, solving the problems of male sterility and seedless breeding in citrus and achieving the breeding goal of seedless citrus.

CN122104742AActive Publication Date: 2026-05-29HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Filing Date
2026-04-28
Publication Date
2026-05-29

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Abstract

The application discloses a gene KNU for controlling navel orange male sterility and application thereof. The nucleotide sequence of the gene KNU for controlling navel orange male sterility is shown as SEQ ID NO:1. The amino acid sequence of a protein KNU for controlling navel orange male sterility encoded by the gene KNU for controlling navel orange male sterility is shown as SEQ ID NO:2. The application identifies a key gene KNU for controlling male sterility and seedlessness from navel orange and its back-mutants. The gene KNU is a gene specifically expressed in anther somatic cells, and through a Cas9 editing mode citrus material, precise breeding of male sterility and seedless citrus can be realized. The application first provides a target gene KNU for controlling citrus male sterility and seedlessness.
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