Method, device and equipment for evaluating effect of groundwater biological stimulation repair

Abstract

The application discloses a storage medium, a groundwater biological stimulation remediation effect evaluation method, device and equipment, wherein the method comprises the following steps: collecting groundwater samples of a target area at multiple preset time points before biological stimulation agents are put; respectively determining monomer isotope values and molecular information of pollutants in the groundwater samples collected each time, and determining abundance data of functional genes and / or key enzymes; calculating the change range of specific indexes including the delta value of monomer isotope, the isotope value of nutrients or electron acceptors, and the abundance data of functional genes and / or key enzymes; and when the change range of the specific indexes is all rising, it is confirmed that the biological stimulation remediation is effective. The application improves the evaluation accuracy of the effectiveness of the biological stimulation method, and thus the remediation effect of the contaminated area to be remediated can be improved, and invalid cost investment can be avoided.

Description

Technical Field

[0001] This invention relates to the field of pollution monitoring, and in particular to methods, apparatus and equipment for evaluating the effectiveness of biostimulation remediation of groundwater. Background Technology

[0002] Biostimulation is a commonly used technique for the remediation of organic pollution. It involves using methods such as adding nutrients, electron acceptors, and surfactants to promote the growth and reproduction of microorganisms, thereby increasing the activity of native microorganisms and enhancing their ability to degrade organic pollutants.

[0003] The growth and reproduction of microorganisms used for the remediation of organic pollution are affected by a variety of factors, and their activity and proliferation trend are uncertain, which in turn leads to uncertainty in the effectiveness of biostimulation.

[0004] For applications that use biostimulation technology to remediate contaminated groundwater, existing technologies generally use pollutant concentration as an indicator, and obtain the effect evaluation results of biostimulation remediation by analyzing the difference in pollutant concentration before and after biostimulation.

[0005] The inventors discovered through research that existing methods for evaluating the effects of biostimulation repair have at least the following drawbacks:

[0006] In practical site applications, changes in pollutant concentration are the result of the combined effects of multiple factors, including bio-stimulated degradation, natural degradation, volatilization, adsorption / desorption, and convection-diffusion. Therefore, using pollutant concentration as an indicator to assess the effectiveness of bio-stimulated remediation of contaminated groundwater will lead to inaccurate assessment results.

[0007] The information disclosed in the background section is intended only to enhance the understanding of the overall background of the present invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0008] The purpose of this invention is to improve the accuracy of evaluating the effects of biostimulation repair.

[0009] This invention provides a method for evaluating the effectiveness of biostimulation remediation of groundwater, comprising the following steps:

[0010] S11. Groundwater samples from the target area are collected at multiple preset time points before and after the administration of the biostimulant.

[0011] S12. Measure the δ value of the monomeric isotopes of pollutants and the δ value of the isotopes of nutrients or electron acceptors in each of the groundwater samples collected; extract biomolecular information including at least nucleic acids from each of the groundwater samples, and measure the abundance data of functional genes and / or key enzymes stimulated by the biostimulant according to the stimulation mechanism of the biostimulant.

[0012] S13. Based on the time points corresponding to each of the groundwater samples, calculate the variation range of specific indicators including the δ value of each monomer isotope, the δ value of the isotope of nutrients or electron acceptors, and the abundance data of functional genes and / or key enzymes stimulated by the biostimulant.

[0013] S14. When the changes in the specific indicators are all increasing, the bio-stimulation repair is confirmed to be effective.

[0014] Preferably, in this invention, it further includes:

[0015] S21. Before administering the biostimulant, collect groundwater and soil samples from the target area and use the groundwater and soil samples to conduct a microcosm experiment under biostimulation measures.

[0016] S22. Determine the concentration of pollutants and the δ value of individual isotopes of pollutants at two different time points in the microcosm experiment, and calculate the fractionation enrichment factor ε of each individual isotope according to the Rayleigh fractionation model of isotopes; the two different time points are the initial moment of the microcosm experiment and the time point after a preset degradation time during the microcosm experiment; the formula used in the isotope Rayleigh fractionation model includes:

[0017]

[0018] In the formula, ε is the fractionation enrichment factor of the isotope; R is the degree of degradation, that is, the ratio of the concentration after a preset degradation time t to the concentration at the initial moment of the microcosm experiment; δ t δ is the δ value (in ‰) of the monomer isotope after a preset degradation time t; δ0 is the δ value (in ‰) of the monomer isotope at the initial moment of the microcosm experiment.

[0019] S23. Based on the isotope fractionation enrichment factor ε, calculate the degree of degradation at each preset time point using formula (2). The calculation includes:

[0020]

[0021] In the formula, δ i δ is the δ value of the isotope at the i-th preset time point, and δ0 is the δ value of the isotope before the release of the biostimulant.

[0022] In another aspect of this invention, an effect evaluation device for biostimulation remediation of groundwater is also provided, comprising:

[0023] The on-site data acquisition unit is used to acquire the δ values ​​of monomeric isotopes of pollutants, the δ values ​​of isotopes of nutrients or electron acceptors in each of the groundwater samples, and the abundance data of functional genes and / or key enzymes stimulated by the biostimulant.

[0024] The variation range calculation unit is used to calculate the variation range of specific indicators, including the δ value of the monomer isotope, the δ value of the nutrient or electron acceptor isotope, and the abundance data of the functional genes and / or key enzymes stimulated by the biostimulant, based on the time point corresponding to each of the groundwater samples.

[0025] The effectiveness judgment unit is used to confirm that the biostimulation repair is effective when the change range of the specific indicators is all increasing.

[0026] Preferably, in this invention, it further includes:

[0027] The experimental data acquisition unit is used to determine the concentration of pollutants and the δ value of individual isotopes of pollutants at two different time points in the microcosm experiment, and to calculate the fractionation enrichment factor ε of each individual isotope according to the Rayleigh fractionation model of isotopes; the two different time points are the initial moment of the microcosm experiment and the time point after a preset degradation time during the microcosm experiment; the formula used in the isotope Rayleigh fractionation model includes:

[0028]

[0029] In the formula, ε is the fractionation enrichment factor of the isotope; R is the degree of degradation, that is, the ratio of the concentration after a preset degradation time t to the concentration at the initial moment of the microcosm experiment; δ t δ is the δ value (in ‰) of the monomer isotope after a preset degradation time t; δ0 is the δ value (in ‰) of the monomer isotope at the initial moment of the microcosm experiment.

[0030] The degradation degree calculation unit is used to calculate the degradation degree at each preset time point according to the isotope fractionation enrichment factor ε using formula (2). The calculation includes:

[0031]

[0032] In the formula, δ iδ is the δ value of the isotope at the i-th preset time point, and δ0 is the δ value of the isotope before the release of the biostimulant. In another aspect of this invention, an effect evaluation device for groundwater biostimulant remediation is also provided; the effect evaluation device for groundwater biostimulant remediation includes a computer program stored on a medium, the computer program includes program instructions, and when the program instructions are executed by the computer, the computer performs the methods described in the above aspects and achieves the same technical effect.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] The inventors discovered through research that the degradation process stimulates the growth of degrading microorganisms. By analyzing the marker biomolecules of these degrading microorganisms, it is possible to determine whether the biostimulation method has a stimulating effect.

[0035] Based on the above understanding, in order to avoid the misleading evaluation of the effectiveness of biostimulation in remediating contaminated groundwater due to fluctuations in pollutant indicators caused by non-biostimulation degradation factors, this invention adopts a method that combines microcosm experimental data with abundance data of functional genes and / or key enzymes stimulated by biostimulants to verify the effectiveness of biostimulation methods. This effectively improves the accuracy of the evaluation of the effectiveness of biostimulation methods, thereby improving the remediation effect of the contaminated area to be remediated and avoiding ineffective cost investment.

[0036] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention and to implement it according to the contents of the specification, and to make the above and other objects, technical features and advantages of the present invention easier to understand, one or more preferred embodiments are listed below and described in detail with reference to the accompanying drawings. Attached Figure Description

[0037] To more clearly illustrate the technical solution of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0038] Figure 1 This is a flowchart illustrating the steps of the method for evaluating the effectiveness of groundwater biostimulation remediation described in this invention.

[0039] Figure 2 This is a schematic diagram of the degradation rate of pollutants at each preset time point in this invention;

[0040] Figure 3This is a bar chart of the functional gene and benzene series anaerobic degradation gene BssA data at various preset time points in this invention.

[0041] Figure 4 This is a schematic diagram of the concentration of pollutants measured in the microcosm experiment of this invention;

[0042] Figure 5 This is a schematic diagram of the concentration of nutrients measured in the microcosm experiment of this invention;

[0043] Figure 6 This is a schematic diagram of the structure of the groundwater biostimulation remediation effect evaluation device described in this invention;

[0044] Figure 7 This is a schematic diagram of the structure of the groundwater biostimulation remediation effect evaluation device described in this invention. Detailed Implementation

[0045] The specific embodiments of the present invention will now be described in detail with reference to the accompanying drawings, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0046] Unless otherwise expressly stated, throughout the specification and claims, the term "comprising" or its variations such as "including" or "comprises" shall be understood to include the stated elements or components without excluding other elements or other components.

[0047] In this document, the terms "first," "second," etc., are used to distinguish two different elements or parts, and are not used to define specific positions or relative relationships. In other words, in some embodiments, the terms "first," "second," etc., can also be used interchangeably.

[0048] Example 1

[0049] To improve the accuracy of evaluating the effects of biostimulation repair, such as Figure 1 As shown in the embodiment of the present invention, a method for evaluating the effect of biostimulation remediation of groundwater is provided, including the following steps:

[0050] S11. Groundwater samples from the target area are collected at multiple preset time points before and after the administration of the biostimulant.

[0051] In this embodiment of the invention, the multiple preset time points for collecting groundwater samples are: one sample is collected before the biostimulant is added, and multiple more time points for collecting groundwater samples are set after the biostimulant is added.

[0052] For example, the preset time points after the biostimulant is administered can be set to 10 days, 20 days, 40 days, 50 days, and 60 days, while the collection time point before the biostimulant is administered can be day 0.

[0053] In practical applications, groundwater samples can be collected from the target area using groundwater monitoring wells; preferably, groundwater monitoring wells can be set up at multiple points of interest in the target area for collecting groundwater samples.

[0054] S12. Measure the δ value of the monomeric isotopes of pollutants and the δ value of the isotopes of nutrients or electron acceptors in each of the groundwater samples collected; extract biomolecular information including at least nucleic acids from each of the groundwater samples, and measure the abundance data of functional genes and / or key enzymes stimulated by the biostimulant according to the stimulation mechanism of the biostimulant.

[0055] The inventive concept of this invention includes: to avoid the misleading evaluation of the effect of biostimulation on the remediation of pollutant groundwater due to fluctuations in pollutant indicators caused by non-biostimulation degradation factors, this invention adopts a method that combines pollutant concentration detection with the abundance data of functional genes and / or key enzymes stimulated by biostimuli to comprehensively judge the effectiveness of biostimulation methods.

[0056] Based on the above inventive concept, in the embodiments of the present invention, for each groundwater sample, while measuring the δ value of the monomer isotope of the dye, the δ value of the isotope of the nutrient or electron acceptor is also measured.

[0057] Furthermore, embodiments of the present invention also extract biomolecular information, including at least nucleic acids, from groundwater samples. Then, based on the stimulation mechanism of the biostimulant, the abundance data of functional genes and / or key enzymes stimulated by the biostimulant are determined. Specifically, this can be achieved by extracting DNA from groundwater samples and using metagenomic analysis to analyze the abundance of biostimulation-related functional genes, such as Nar and Nir abundances when nitrate acts as an electron acceptor; and pollutant oxidation genes, such as the abundance of the benzene series anaerobic degradation gene BssA.

[0058] In practical applications, the pollutants in the embodiments of this invention generally refer to organic pollutants, such as organic pollutants including toluene.

[0059] S13. Based on the time points corresponding to each of the groundwater samples, calculate the variation range of specific indicators including the δ value of the monomer isotope, the δ value of the isotope of the nutrient or electron acceptor, and the abundance data of the functional genes and / or key enzymes stimulated by the biostimulant.

[0060] Taking preset time points of 0 days, 10 days, 20 days, 40 days, 50 days, and 60 days as examples: Assume the pollutants in the groundwater sample are organic pollutants including toluene. The functional genes stimulated by biostimulants include: electron acceptor reduction genes, including Nar and Nir; and organic matter oxidation genes, including AlkB and BssA.

[0061] The key enzymes stimulated by the biostimulant in the embodiments of the present invention include enzymes encoded by functional genes stimulated by the biostimulant.

[0062] Based on the measurement data obtained at each preset time point, it can be as follows: Figure 2 and Figure 3 As shown, where, Figure 2 This is a schematic diagram showing the pollutant degradation rate at each preset time point based on the δ value of the monomer isotope.

[0063] Figure 3 A bar chart showing the functional genes and the benzene series anaerobic degradation gene BssA at each preset time point.

[0064] S14. When the changes in the specific indicators are all increasing, the bio-stimulation repair is confirmed to be effective.

[0065] When the δ value of the monomeric isotope of a pollutant increases, it can be determined whether the pollutant has been significantly degraded. However, it is still impossible to determine whether the degradation effect is caused by biostimulation. Therefore, in this embodiment of the invention, it is also necessary to analyze the δ value of the isotope of nutrients or electron acceptors, as well as the abundance data of functional genes and / or key enzymes stimulated by the biostimulating agent. When the change in specific indicators is all increasing, the biostimulation repair is confirmed to be effective.

[0066] For example, when the monomeric isotope of a pollutant (such as toluene) δ 13 C value, electron acceptor (e.g., nitrate) isotope (δ) 15 The abundance of N and stimulated functional genes (such as Nar, Nir and BssA) increased, confirming the effectiveness of this biostimulation repair.

[0067] Furthermore, in embodiments of the present invention, a step of quantitatively evaluating the effect of the biostimulation may be included, comprising the following steps:

[0068] S21. Before administering the biostimulant, collect groundwater and soil samples from the target area and use the groundwater and soil samples to conduct a microcosm experiment under biostimulation measures.

[0069] In practical applications, the timing of collecting groundwater and soil samples from the target area can be the same as the timing of collecting groundwater samples before the application of biostimulants.

[0070] S22. Determine the concentration of pollutants and the δ value of individual isotopes of pollutants at two different time points in the microcosm experiment, and calculate the fractionation enrichment factor ε of each individual isotope according to the Rayleigh fractionation model of isotopes; the two different time points are the initial moment of the microcosm experiment and the time point after a preset degradation time during the microcosm experiment; the formula used in the isotope Rayleigh fractionation model includes:

[0071]

[0072] In the formula, ε is the fractionation enrichment factor of the monomer isotope; R is the degree of degradation, i.e., the ratio of the concentration after a preset degradation time t to the concentration at the initial moment of the microcosm experiment; δ t δ is the δ value of the monomer isotope after a preset degradation time t (in ‰); δ0 is the δ value of the monomer isotope at the initial moment of the microcosm experiment (in ‰).

[0073] In practical applications, refer to Figure 4 A schematic diagram showing the concentration of pollutants measured in the microcosm experiment, and... Figure 5 A schematic diagram of the concentration of nutrients (i.e., biostimulants) measured in the microcosm experiment. After obtaining the δ values ​​of the monomeric isotopes at the initial moment of the microcosm experiment and at the time point after a preset degradation time during the microcosm experiment, the fractionation enrichment factor ε of each monomeric isotope can be calculated using the Rayleigh fractionation model of isotopes.

[0074] It should be noted that in this step, by measuring the concentration of pollutants at two different time points in the microcosm experiment, the feasibility of the biostimulation method in this embodiment of the invention can be determined. Therefore, this step can serve as a preliminary step for implementing biostimulation (administering biostimulant agents) in the target area. It is used to determine whether to implement biostimulation or to select a specific plan for implementing biostimulation.

[0075] In practical applications, it can be used to determine the monomeric isotope delta of toluene in groundwater. 13 C value, and / or, nitrate nitrogen δ 15 N value; In a specific example of the present invention, the monomeric isotope δ of toluene in a microcosm experiment was determined. 13 The C-value, based on the Rayleigh fractionation model, is used to calculate the toluene degradation δ in this environment. 13 The enrichment factor ε for C isotope fractionation is -0.94‰.

[0076] S23. Based on the isotope fractionation enrichment factor ε, calculate the degree of degradation at each preset time point using formula (2). The calculation includes:

[0077]

[0078] In the formula, δ i δ is the δ value of the isotope at the i-th preset time point, and δ0 is the δ value of the isotope before the release of the biostimulant.

[0079] After obtaining the isotopic fractionation enrichment factor ε of the pollutants, the degree of degradation of the pollutants caused by biostimulation can be calculated based on the δ value of the isotopes obtained from the measured groundwater samples, thereby realizing the quantitative assessment of biostimulation.

[0080] In summary, to avoid misleading evaluations of the effectiveness of biostimulation in remediating contaminated groundwater due to fluctuations in pollutant indicators caused by non-biostimulation degradation factors, this invention employs a method combining microcosmic experimental data with abundance data of functional genes and / or key enzymes stimulated by biostimulants to verify the effectiveness of biostimulation methods. This effectively improves the accuracy of the evaluation of the effectiveness of biostimulation methods, thereby enhancing the remediation effect of the contaminated area and avoiding ineffective cost investments.

[0081] Example 2

[0082] Corresponding to the method embodiment, another aspect of the present invention provides an effect evaluation device for biostimulation remediation of groundwater. Figure 6 This diagram illustrates the structure of a groundwater biostimulation remediation effect evaluation device provided in an embodiment of the present invention. The groundwater biostimulation remediation effect evaluation device is a... Figure 1 The device corresponding to the groundwater biostimulation remediation effect evaluation method described in the corresponding embodiment is implemented through a virtual device. Figure 1 The groundwater biostimulation remediation effect evaluation method in the corresponding embodiment, wherein each virtual module constituting the groundwater biostimulation remediation effect evaluation device can be executed by electronic devices, such as network devices, terminal devices, or servers. Specifically, the groundwater biostimulation remediation effect evaluation device in the embodiment of the present invention includes:

[0083] The on-site data acquisition unit 01 is used to acquire the δ values ​​of monomeric isotopes of pollutants, the δ values ​​of isotopes of nutrients or electron acceptors in each of the groundwater samples, and the abundance data of functional genes and / or key enzymes stimulated by the biostimulant.

[0084] The variation range calculation unit 02 is used to calculate the variation range of specific indicators, including the δ value of the monomer isotope, the δ value of the nutrient or electron acceptor isotope, and the abundance data of the functional genes and / or key enzymes stimulated by the biostimulant, based on the time point corresponding to each of the groundwater samples.

[0085] The effectiveness judgment unit 03 is used to determine whether the biostimulation repair is effective based on whether the change range of the specific indicators exceeds their respective preset thresholds.

[0086] Preferably, in embodiments of the present invention, it may further include:

[0087] The experimental data acquisition unit (not shown in the figure) is used to determine the concentration of pollutants and the δ value of the monomeric isotopes of the pollutants at two different time points in the microcosm experiment, and to calculate the fractionation enrichment factor ε of each monomeric isotope according to the Rayleigh fractionation model of isotopes; the two different time points are the initial moment of the microcosm experiment and the time point after a preset degradation time during the microcosm experiment; the formula used in the isotope Rayleigh fractionation model includes:

[0088]

[0089] In the formula, ε is the fractionation enrichment factor of the isotope; R is the degree of degradation, that is, the ratio of the concentration after a preset degradation time t to the concentration at the initial moment of the microcosm experiment; δ t δ is the δ value (in ‰) of the monomer isotope after a preset degradation time t; δ0 is the δ value (in ‰) of the monomer isotope at the initial moment of the microcosm experiment.

[0090] The degradation degree calculation unit (not shown in the figure) is used to calculate the degradation degree at each preset time point according to the isotope fractionation enrichment factor ε using formula (2). The calculation includes:

[0091]

[0092] In the formula, δ i δ is the δ value of the isotope at the i-th preset time point, and δ0 is the δ value of the isotope before the release of the biostimulant.

[0093] It should be noted that the specific implementation method and technical effects of the groundwater biostimulation remediation effect evaluation device in the embodiments of the present invention can be referred to Figure 1 The corresponding methods for evaluating the effectiveness of groundwater biostimulation remediation will not be elaborated here.

[0094] Example 3

[0095] Corresponding to the method embodiments, this invention also provides an effect evaluation device for groundwater biostimulation remediation, such as a terminal or server. The server can be a standalone physical server, a server cluster or distributed system composed of multiple physical servers, or a cloud server providing basic cloud computing services such as cloud services, cloud databases, cloud computing, cloud functions, cloud storage, network services, cloud communication, middleware services, domain name services, security services, CDN, and big data and artificial intelligence platforms. The terminal can be a smartphone, tablet, laptop, desktop computer, etc., but is not limited to these.

[0096] An example diagram of the hardware structure block diagram of the groundwater biostimulation remediation effect evaluation device provided in this application embodiment is shown below. Figure 7 As shown, it may include:

[0097] Processor 1, communication interface 2, memory 3, and communication bus 4;

[0098] The processor 1, communication interface 2, and memory 3 communicate with each other via communication bus 4.

[0099] Optionally, communication interface 2 can be an interface of a communication module, such as the interface of a GSM module;

[0100] Processor 1 may be a central processing unit (CPU), an application-specific integrated circuit (ASIC), or one or more integrated circuits configured to implement the embodiments of this application.

[0101] Memory 3 may include high-speed RAM memory, and may also include non-volatile memory, such as at least one disk storage device.

[0102] Specifically, processor 1 is used to execute the computer program stored in memory 3 to perform the following steps:

[0103] S11. Groundwater samples from the target area are collected at multiple preset time points before and after the administration of the biostimulant.

[0104] S12. Measure the δ value of the monomeric isotopes of pollutants and the δ value of the isotopes of nutrients or electron acceptors in each of the groundwater samples collected; extract biomolecular information including at least nucleic acids from each of the groundwater samples, and measure the abundance data of functional genes and / or key enzymes stimulated by the biostimulant according to the stimulation mechanism of the biostimulant.

[0105] S13. Based on the time points corresponding to each of the groundwater samples, calculate the variation range of specific indicators including the δ value of each monomer isotope, the δ value of the isotope of nutrients or electron acceptors, and the abundance data of functional genes and / or key enzymes stimulated by the biostimulant.

[0106] S14. When the change range of the specific indicators all exceeds their respective preset thresholds, the bio-stimulation repair is confirmed to be effective.

[0107] Preferably, in this invention, it further includes:

[0108] S21. Before administering the biostimulant, collect groundwater and soil samples from the target area and use the groundwater and soil samples to conduct a microcosm experiment under biostimulation measures.

[0109] S22. Determine the concentration of pollutants and the δ value of individual isotopes of pollutants at two different time points in the microcosm experiment, and calculate the fractionation enrichment factor ε of each individual isotope according to the Rayleigh fractionation model of isotopes; the two different time points are the initial moment of the microcosm experiment and the time point after a preset degradation time during the microcosm experiment; the formula used in the isotope Rayleigh fractionation model includes:

[0110]

[0111] In the formula, ε is the fractionation enrichment factor of the isotope; R is the degree of degradation, that is, the ratio of the concentration after a preset degradation time t to the concentration at the initial moment of the microcosm experiment; δ t δ is the δ value (in ‰) of the monomer isotope after a preset degradation time t; δ0 is the δ value (in ‰) of the monomer isotope at the initial moment of the microcosm experiment.

[0112] S23. Based on the isotope fractionation enrichment factor ε, calculate the degree of degradation at each preset time point using formula (2). The calculation includes:

[0113]

[0114] In the formula, δ i δ is the δ value of the isotope at the i-th preset time point, and δ0 is the δ value of the isotope before the release of the biostimulant.

[0115] The above-described product can perform the method provided in the embodiments of the present invention, and has the corresponding functional modules and beneficial effects for performing the method. For technical details not described in detail in this embodiment, please refer to the biostimulation repair effect evaluation method provided in the embodiments of the present invention.

[0116] Those skilled in the art will recognize that the units and algorithm steps of the various examples described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are implemented in hardware or software depends on the specific application and design constraints of the technical solution. Those skilled in the art can use different methods to implement the described functions for each specific application, but such implementation should not be considered beyond the scope of this application.

[0117] In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods can be implemented in other ways. Furthermore, the couplings or direct couplings or communication connections shown or discussed may be indirect couplings or communication connections through interfaces, devices, or units, and may be electrical, mechanical, or other forms.

[0118] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0119] In addition, the functional units in the various embodiments of this application can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit.

[0120] It should be understood that in the embodiments of this application, the claims, various embodiments, and features can be combined with each other to solve the aforementioned technical problems.

[0121] If the aforementioned functions are implemented as software functional units and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, or a portion of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of this application. The aforementioned storage medium includes various media capable of storing program code, such as USB flash drives, portable hard drives, read-only memory (ROM), random access memory (RAM), magnetic disks, or optical disks.

[0122] The above description of the disclosed embodiments enables those skilled in the art to make or use this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for evaluating the effectiveness of biostimulation remediation of groundwater, characterized in that, Including the following steps: S11. Groundwater samples from the target area are collected at multiple preset time points before and after the administration of the biostimulant. S12. Measure the δ value of the monomeric isotopes of pollutants and the δ value of the isotopes of nutrients or electron acceptors in each of the groundwater samples collected; extract biomolecular information including at least nucleic acids from each of the groundwater samples, and measure the abundance data of functional genes and / or key enzymes stimulated by the biostimulant according to the stimulation mechanism of the biostimulant. S13. Based on the time points corresponding to each of the groundwater samples, calculate the variation range of specific indicators including the δ value of the monomer isotope, the δ value of the isotope of the nutrient or electron acceptor, and the abundance data of the functional genes and / or key enzymes stimulated by the biostimulant. S14. When the changes in the specific indicators are all increasing, the bio-stimulation repair is confirmed to be effective.

2. The method for evaluating the effect of groundwater biostimulation remediation according to claim 1, characterized in that, Also includes: S21. Before administering the biostimulant, collect groundwater and soil samples from the target area and use the groundwater and soil samples to conduct a microcosm experiment under biostimulation measures. S22. Determine the concentration of pollutants and the δ value of the monomeric isotopes of pollutants at two different time points in the microcosm experiment, and calculate the fractionation enrichment factor ε of each monomeric isotope according to the Rayleigh fractionation model of isotopes. The two different time points are the initial moment of the microcosm experiment and the time point after the preset degradation time during the microcosm experiment; The formulas used in the isotope Rayleigh fractionation model include: In the formula, ε is the fractionation enrichment factor of the isotope; R is the degree of degradation, that is, the ratio of the concentration after a preset degradation time t to the concentration at the initial moment of the microcosm experiment; δ t δ is the δ value (in ‰) of the monomer isotope after a preset degradation time t; δ0 is the δ value (in ‰) of the monomer isotope at the initial moment of the microcosm experiment. S23. Based on the isotope fractionation enrichment factor ε, calculate the degree of degradation at each preset time point using formula (2). The calculation includes: In the formula, δ i δ is the δ value of the isotope at the i-th preset time point, and δ0 is the δ value of the isotope before the release of the biostimulant.

3. The method for evaluating the effect of groundwater biostimulation remediation according to claim 2, characterized in that, The groundwater samples collected from the target area include: Groundwater samples were collected from the target area in the groundwater monitoring well.

4. The method for evaluating the effect of groundwater biostimulation remediation according to claim 3, characterized in that, The groundwater monitoring wells include multiple wells and are located at points of interest in the target area.

5. The method for evaluating the effect of groundwater biostimulation remediation according to claim 4, characterized in that, The pollutants include: Organic pollutants, including toluene.

6. The method for evaluating the effect of groundwater biostimulation remediation according to claim 5, characterized in that, The functional genes stimulated by the biostimulant include: Electron acceptor reduction genes, including: Nar, Nir; Organic oxidation genes include: AlkB and BssA.

7. The method for evaluating the effect of groundwater biostimulation remediation according to claim 6, characterized in that, The key enzymes stimulated by the biostimulant include: The enzyme encoded by the functional gene stimulated by the biostimulant.

8. The method for evaluating the effect of groundwater biostimulation remediation according to claim 7, characterized in that, The determination of abundance data of functional genes and / or key enzymes stimulated by the biostimulant, based on the stimulation mechanism of the biostimulant, includes: DNA was extracted from the groundwater sample, and metagenomic analysis was used to analyze the functional genes related to biostimulation and pollutant oxidation.

9. The method for evaluating the effect of groundwater biostimulation remediation according to claim 8, characterized in that, The confirmation that the biostimulation repair is effective when all the changes in the specific indicators are increasing includes: When the monomeric isotope δ 13 C value, electron acceptor isotope δ 15 N. The abundance of the stimulated functional genes all increased, confirming the effectiveness of this biostimulation repair.

10. An apparatus for evaluating the effectiveness of biostimulation-based remediation of groundwater, used to implement the method for evaluating the effectiveness of biostimulation-based remediation of groundwater as described in any one of claims 1 to 9, characterized in that, include: The on-site data acquisition unit is used to acquire the δ values ​​of monomeric isotopes of pollutants, the δ values ​​of isotopes of nutrients or electron acceptors in each of the groundwater samples, and the abundance data of functional genes and / or key enzymes stimulated by the biostimulant. The variation range calculation unit is used to calculate the variation range of specific indicators, including the δ value of the monomer isotope, the δ value of the nutrient or electron acceptor isotope, and the abundance data of the functional genes and / or key enzymes stimulated by the biostimulant, based on the time point corresponding to each of the groundwater samples. The effectiveness judgment unit is used to confirm that the biostimulation repair is effective when the change range of the specific indicators is all increasing.

11. The groundwater biostimulation remediation effect evaluation device according to claim 10, characterized in that, Also includes: The experimental data acquisition unit is used to determine the concentration of pollutants and the δ value of the monomeric isotopes of pollutants at two different time points in the microcosm experiment, and to calculate the fractionation enrichment factor ε of each monomeric isotope according to the Rayleigh fractionation model of isotopes. The two different time points are the initial moment of the microcosm experiment and the time point after the preset degradation time during the microcosm experiment; The formulas used in the isotope Rayleigh fractionation model include: In the formula, ε is the fractionation enrichment factor of the isotope; R is the degree of degradation, that is, the ratio of the concentration after a preset degradation time t to the concentration at the initial moment of the microcosm experiment; δ t δ is the δ value (in ‰) of the monomer isotope after a preset degradation time t; δ0 is the δ value (in ‰) of the monomer isotope at the initial moment of the microcosm experiment. The degradation degree calculation unit is used to calculate the degradation degree at each preset time point according to the isotope fractionation enrichment factor ε using formula (2). The calculation includes: In the formula, δ i δ is the δ value of the isotope at the i-th preset time point, and δ0 is the δ value of the isotope before the release of the biostimulant.

12. A device for evaluating the effectiveness of groundwater biostimulation remediation, characterized in that, include: Memory, used to store computer programs; A processor is configured to invoke and execute the computer program to implement the steps of the method for evaluating the effectiveness of groundwater biostimulation remediation as described in any one of claims 1-9.

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