An immunodetection device with sampling and immersion functions
By designing an immunoassay device that combines sampling and immersion functions, the impact of test strip immersion time and length on test results has been resolved, achieving efficient and accurate test results and simplifying the operation process.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
- Filing Date
- 2026-04-08
- Publication Date
- 2026-05-29
AI Technical Summary
In immunoassay, factors such as the immersion time and length of the test strip affect the accuracy and efficiency of the test results, and existing technologies are inconvenient to operate.
Design an immunoassay device that combines sampling and immersion functions, comprising a base, an immersion rod, a timed reset device, and a replacement structure. The immersion time of the test strip is controlled by a timer, and the test strip can be quickly replaced by the replacement structure.
It improves the accuracy and efficiency of testing, reduces operational errors, simplifies the operation process, and enhances the convenience of testing.
Smart Images
Figure CN122109527A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of immunoassay technology, specifically relating to an immunoassay device that combines sampling and immersion functions. Background Technology
[0002] In the daily work of hospital laboratory departments, immunoassay is one of the core testing items, widely used for the detection of trace biomolecules such as tumor markers, hormones, myocardial markers, and infectious disease markers. It provides crucial laboratory evidence for disease diagnosis, condition assessment, and treatment monitoring, and is an indispensable component of modern laboratory medicine. With the continuous development of medical technology, the sample volume in laboratory departments has increased significantly, especially in tertiary hospitals and large medical laboratories, which process thousands or even tens of thousands of samples daily. This places increasingly higher demands on testing efficiency, result accuracy, and ease of operation. In immunochromatography, test strips are commonly used. Typically, specific antibodies or antigens are pre-fixed on a paper carrier. The condition of the target being tested is determined by the visual reaction (such as color change) between the sample and the antibodies or antigens on the test strip. Commonly used bare test strips require the operator to immerse the strip in the sample for a period of time and then lift it out to wait for color development. However, in this operation, the color development of the test strip is affected by factors such as immersion time and immersion length, which can easily lead to distortion or misreading of the test strip, affecting the test results. Summary of the Invention
[0003] In view of this, the present invention discloses an immunoassay device that combines sampling and immersion functions, the purpose of which is to solve the problem that the test strip is affected by factors such as immersion time during operation.
[0004] To achieve the above objectives, the present invention provides the following technical solution: An immunoassay device combining sampling and immersion functions includes a base, a placement ring at the bottom of the base, and a disposable sampling cup placed within the placement ring; an installation cylinder is provided on the base, and an immersion rod is vertically slidably mounted at the top of the installation cylinder, facing the placement ring, with a timed reset device between the immersion rod and the installation cylinder; a test strip is mounted on the immersion rod, and a replacement structure for replacing the test strip is provided inside the installation cylinder; a through groove is opened at the bottom of the installation cylinder, facing the immersion rod; and an observation window is opened on the side wall of the installation cylinder for observing changes in the test strip.
[0005] In this procedure, the sample to be tested is placed in a disposable sampling cup, which is then placed inside a sampling ring. The test strip is then mounted on the immersion rod via a replacement mechanism. The test strip is immersed in the sample by pressing down the immersion rod until the detection end of the test strip is submerged in the sampling liquid for a certain length. The immersion rod is then released, and a timer reset device is used to set the immersion time. After the set immersion time is completed, the timer reset device resets the immersion rod. The color development of the test strip is then observed through the observation window. In this procedure, during multiple tests in the laboratory, only the disposable sampling cup and the test strip need to be replaced via the replacement mechanism. The entire operation quantifies external factors, avoiding errors caused by non-standard manual operation, making the operation more convenient, and improving testing efficiency and result accuracy.
[0006] Furthermore, the replacement structure includes a rotating shaft coaxially rotatably connected to the bottom of the mounting cylinder. Several horizontal bars are distributed in a circular array around the rotating shaft. A support plate is vertically slidably connected to the end of each horizontal bar. The side of the support plate facing the inner wall of the mounting cylinder is used to place the test strip. A support rod is provided on the side wall of the support plate. A pressing block for pressing the test strip is rotatably connected to each support rod, and a torsion spring is provided at the connection. Limiting grooves are opened on both sides of the top of the support plate. A groove is opened at the bottom of the immersion rod. Limiting rods facing the limiting grooves are slidably arranged on both sides of the groove. The ends of the limiting rods are arc-shaped, and elastic reset members are provided between the limiting rods and the grooves.
[0007] In this solution, test strips are placed on support plates beforehand and pressed and fixed by clamping plates. Simply rotate the support shaft to slide one support plate into the groove of the immersion rod, pushing the limiting rod. When the limiting rod aligns with the limiting groove on the support plate, it slides into the limiting groove under the action of the corresponding elastic reset element. When the shaft experiences resistance, the test strip replacement is complete. After use, simply rotate the shaft again to connect the next support plate to the immersion rod, enabling rapid test strip replacement and further improving detection efficiency.
[0008] Furthermore, the positioning and resetting structure includes a resetting rod and a positioning rod fixed to the immersion rod, both the timing rod and the resetting rod being L-shaped. A first hydraulic cylinder, fixedly connected to the mounting cylinder, is coaxially sleeved on the vertical section of the resetting rod. A hydraulic plate, connected to the end of the resetting rod, is slidably connected inside the first hydraulic cylinder. The hydraulic plate has an overflow hole, and an elastic resetting element is also provided between the hydraulic plate and the first hydraulic cylinder. A toothed plate is fixed on the vertical section of the positioning rod. A horizontally oriented second hydraulic cylinder passes through the mounting cylinder. An adjusting shaft is slidably connected coaxially inside the second hydraulic cylinder, and a pressure plate, coaxially fixed to the adjusting shaft, is slidably connected inside the second hydraulic cylinder. An overflow hole is also provided on the pressure plate. An elastic reset component is also provided between the adjusting shaft and the second hydraulic cylinder. One end of the adjusting shaft extends out of the mounting cylinder, and an annular groove is provided on the circumference of the other end of the adjusting shaft. Several guide grooves are provided on the side of the annular groove away from the mounting cylinder. An annular block is rotatably and slidably connected to the other end of the adjusting shaft. Several guide blocks are provided on the inner wall of the annular block and slidably connected to the guide grooves. Two stops for clamping the annular block are provided on the inner wall of the mounting cylinder. Several pawls that mesh with the toothed plate are hinged on the outer wall of the annular block, and a torsion spring is provided at the connection. One-way limiting blocks that are fixedly connected to the annular block are fitted to the outer arc surface of the pawls.
[0009] In this scheme, when the immersion rod moves downward, it drives the timing rod and the reset rod to move downward synchronously; at the same time, it pulls the adjustment shaft outward, and the adjustment shaft drives the pressure plate and squeezes the corresponding elastic reset component to accumulate a certain amount of elastic potential energy and then remains stationary. The release time of the accumulated elastic potential energy is the set test paper immersion time; at this time, the guide block slides from the annular groove into the corresponding guide groove, so that the annular block cannot rotate. The timing lever drives the hydraulic plate to compress the elastic reset component, accumulating elastic potential energy. The reset lever then drives the toothed plate to move downwards, at which point the toothed plate presses down on the corresponding pawl, causing it to deflect downwards. Once the test paper has been immersed in the sample to the required length, the immersion lever and the adjusting shaft are released. At this point, the corresponding pawl is restricted from upward deflection by the one-way limiting block, preventing the immersion lever from moving upwards. The adjusting shaft is then reset by the corresponding elastic reset component. Due to the flow of hydraulic oil in the second hydraulic cylinder through the overflow hole, the adjusting shaft resets slowly. After the set immersion time ends, the adjusting shaft is fully reset. At this point, the guide block slides from the guide groove into the annular groove, allowing the annular block to rotate. This, in turn, allows the immersion lever to drive the pawl and the annular block to rotate. At this point, the elastic reset component in the first hydraulic cylinder releases its elastic potential energy, causing the hydraulic plate, positioning lever, and immersion lever to slowly reset upwards, thereby moving the test paper upwards to the observation window.
[0010] Furthermore, the rotating shaft passes through the mounting cylinder upwards and is rotatably connected to it, and a rotating handle is fixed at the top of the rotating shaft.
[0011] Furthermore, the side wall of the mounting cylinder is provided with a window for replacing the test strip.
[0012] Furthermore, a timing scale line is provided on the periphery of the end of the adjusting shaft extending out of the mounting cylinder, and an immersion indicator block is vertically slidably connected to the side wall of the support plate, with a positioning pin provided between the immersion indicator block and the side wall of the support plate.
[0013] Furthermore, the second cylinder end is provided with a plurality of wedge-shaped clamping blocks that fit against the adjusting shaft, and the outer wall of the clamping blocks is threaded with fastening nuts.
[0014] Other advantages, objectives, and features of the invention will be set forth in the following description and will be apparent to those skilled in the art in some respects, or may be learned by practice of the invention. The objectives and other advantages of the invention can be realized and obtained through the following description. Attached Figure Description
[0015] To make the objectives, technical solutions, and beneficial effects of this invention clearer, the following figures are provided for illustration: Figure 1 This is a schematic diagram of the structure of an embodiment of the present invention; Figure 2 This is a schematic diagram of the internal structure of the mounting cylinder in an embodiment of the present invention; Figure 3 for Figure 2 Enlarged view of point A in the middle; Figure 4 for Figure 2 Enlarged view of point B in the middle; Figure 5 This is a longitudinal sectional view of the first hydraulic cylinder and the second hydraulic cylinder in an embodiment of the present invention; Figure 6 for Figure 5 Enlarged view of point C in the middle; Figure 7 This is a longitudinal sectional view of the connection between the immersion rod and the support plate.
[0016] The following are the markings in the attached diagram: 1. Base; 2. Placement ring; 3. Disposable sampling cup; 4. Mounting cylinder; 5. Immersion rod; 6. Observation window; 7. Rotating shaft; 8. Crossbar; 9. Support plate; 10. Clamping block; 11. Limiting groove; 12. Limiting rod; 13. Reset rod; 14. Positioning rod; 15. First hydraulic cylinder; 16. Hydraulic plate; 17. Overflow hole; 18. Elastic reset component; 19. Toothed plate; 20. Adjusting shaft; 21. Second hydraulic cylinder; 22. Annular block; 23. Pawl; 24. Stop block; 25. Annular groove; 26. Guide groove; 27. Guide block; 28. One-way limiting block; 29. Rotating handle; 30. Window; 31. Immersion indicator block; 32. Positioning pin; 33. Clamping block; 34. Fastening nut; 35. Snap ring. Detailed Implementation
[0017] like Figures 1-7As shown: An immunoassay device combining sampling and immersion functions includes a base, a placement ring 2 at the bottom of the base, and a disposable sampling cup 3 placed inside the placement ring 2; an installation cylinder 4 is provided on the base, and an immersion rod 5 is vertically slidably mounted on the top of the installation cylinder 4, facing the placement ring 2, and a timed reset device is provided between the immersion rod 5 and the installation cylinder 4; a test strip is placed on the immersion rod 5, and a replacement structure for replacing the test strip is provided inside the installation cylinder 4; a through groove is opened at the bottom of the installation cylinder 4, facing the immersion rod 5; and an observation window 6 is opened on the side wall of the installation cylinder 4 for observing changes in the test strip.
[0018] In this procedure, the sample to be tested is placed in a disposable sampling cup 3, which is then placed inside a sampling ring. The test strip is then mounted on the immersion rod 5 via a replacement mechanism. The test strip is immersed in the sample by pressing down on the immersion rod 5, ensuring the detection end of the test strip is submerged in the sampling liquid for a certain distance before releasing the immersion rod 5. An immersion time is set using a timer reset device. After the set immersion time is completed, the timer reset device resets the immersion rod 5. The color development of the test strip is then observed through the observation window 6. In this procedure, during multiple tests in the laboratory, only the disposable sampling cup 3 needs to be replaced, and the test strip needs to be replaced via a replacement mechanism. The entire operation quantifies external factors, avoiding errors caused by non-standard manual operation, making the operation more convenient, and improving testing efficiency and result accuracy.
[0019] Furthermore, the replacement structure includes a rotating shaft 7 coaxially rotatably connected to the bottom of the mounting cylinder 4. Several horizontal bars 8 are distributed in a circular array around the rotating shaft 7. A support plate 9 is vertically slidably connected to the end of each horizontal bar 8. The side of the support plate 9 facing the inner wall of the mounting cylinder 4 is used to place the test strip. A support rod (a conventional technique, so not shown in the figure) is provided on the side wall of the support plate 9. Each support rod is rotatably connected to a pressing block 10 for pressing down the test strip, and a torsion spring (a conventional technique, so not shown in the figure) is provided at the connection. A U-shaped retaining ring 35 is provided on the top block of the support plate 9 to apply support force to the top of the test strip to prevent the top of the test strip from bending and affecting the detection. Limiting grooves 11 are opened on both sides of the top of the support plate 9. A groove is opened at the bottom of the immersion rod 5. Limiting rods 12 facing the limiting grooves 11 are slidably arranged on both sides of the groove. The ends of the limiting rods 12 are arc-shaped, and elastic reset members 18 are provided between the limiting rods 12 and the grooves.
[0020] In this scheme, test strips are placed on support plates 9 beforehand and pressed and fixed by clamping plates. Simply rotate the support shaft to slide one support plate 9 into the groove of the immersion rod 5, pushing the limiting rod 12. When the limiting rod 12 is aligned with the limiting groove 11 on the support plate 9, it slides into the limiting groove 11 under the action of the corresponding elastic reset member 18. When the rotating shaft 7 feels a certain resistance, the test strip replacement is complete. After the test strip is used, simply rotate the rotating shaft 7 again to connect the next support plate 9 to the immersion rod 5, enabling rapid test strip replacement and further improving detection efficiency. It also allows for the replacement of the support plate 9 used for immersion, preventing sample contamination on the support plate 9 and affecting the test results of the next test strip.
[0021] Furthermore, the positioning and resetting structure includes a resetting rod 13 and a positioning rod 14 fixed to the immersion rod 5, and both the timing rod and the resetting rod 13 are L-shaped. A first hydraulic cylinder 15, which is fixedly connected to the mounting cylinder 4, is coaxially sleeved on the vertical section of the resetting rod 13. A hydraulic plate 16, which is connected to the end of the resetting rod 13, is slidably connected inside the first hydraulic cylinder 15. An overflow hole 17 is provided on the hydraulic plate 16. An elastic resetting element 18 is also provided between the hydraulic plate 16 and the first hydraulic cylinder 15. A toothed plate 19 is fixed on the vertical section of the positioning rod 14. A horizontally oriented second hydraulic cylinder 21 is provided through the mounting cylinder 4. An adjusting shaft 20 is slidably connected coaxially inside the second hydraulic cylinder 21. A pressure plate, which is coaxially fixed to the adjusting shaft 20, is slidably connected inside the second hydraulic cylinder 21. The pressure plate is also provided with... An overflow hole 17 is provided. An elastic reset member 18 is also provided between the adjusting shaft 20 and the second hydraulic cylinder 21. One end of the adjusting shaft 20 extends out of the mounting cylinder 4. An annular groove 25 is provided on the circumference of the other end of the adjusting shaft 20. Several guide grooves 26 are provided on the side of the annular groove 25 away from the mounting cylinder 4. An annular block 22 is rotatably and slidably connected to the other end of the adjusting shaft 20. Several guide blocks 27 that are slidably connected to the guide grooves 26 are provided on the inner wall of the annular block 22. Two stops 24 for clamping the annular block 22 are provided on the inner wall of the mounting cylinder 4. Several pawls 23 that mesh with the toothed plate 19 are hinged on the outer wall of the annular block 22, and a torsion spring (a conventional technical means, so it is not shown in the figure) is provided at the connection. One-way limiting blocks 28 that are fixedly connected to the annular block 22 are provided on the outer arc surface of the pawls 23.
[0022] In this scheme, when the immersion rod 5 moves downward, it drives the timing rod and the reset rod 13 to move downward synchronously; at the same time, it pulls the adjusting shaft 20 outward, and the adjusting shaft 20 drives the pressure plate and squeezes the corresponding elastic reset member 18 to accumulate elastic potential energy and then remain stationary. The release time of the accumulated elastic potential energy is the set test paper immersion time; at this time, the guide block 27 slides from the annular groove 25 into the corresponding guide groove 26, so that the annular block 22 cannot rotate. The timing rod drives the hydraulic plate 16 to squeeze the elastic reset member 18 to accumulate elastic potential energy; the reset rod 13 drives the toothed plate 19 to move downward, and at this time, the toothed plate 19 squeezes the corresponding pawl 23 downward to make it deflect downward; when the length of the test paper immersed in the sample reaches the requirement, the immersion rod 5 and the adjusting shaft 20 are released; at this time, the corresponding pawl 23 is restricted from deflecting upward under the action of the one-way limit block 28, so that the immersion rod 5 cannot move upward. The adjusting shaft 20 is reset under the action of the corresponding elastic reset member 18, because the second hydraulic cylinder 21 is inside Hydraulic oil flows through overflow hole 17, causing adjustment shaft 20 to reset slowly. After the set immersion time is completed, adjustment shaft 20 is fully reset. At this time, guide block 27 slides from guide groove 26 into annular groove 25, allowing annular block 22 to rotate. This allows immersion rod 5 to drive pawl 23 and annular block 22 to rotate. At this time, elastic reset component 18 in first hydraulic cylinder 15 releases elastic potential energy, causing hydraulic plate 16, positioning rod 14 and immersion rod 5 to slowly reset upward, thereby moving test paper upward to observation window 6.
[0023] Furthermore, the rotating shaft 7 extends upward through the mounting cylinder 4 and is rotatably connected to it, and a rotating handle 29 is fixed at the top of the rotating shaft 7.
[0024] By setting the rotating handle 29, the rotating shaft 7 can be rotated more effectively.
[0025] Furthermore, the side wall of the mounting cylinder 4 is provided with a window 30 for replacing the test paper.
[0026] By setting window 30, the used test strip can be replaced while testing is being performed, reducing the testing waiting time.
[0027] Furthermore, a timing scale line is provided on the periphery of the end of the adjusting shaft 20 extending out of the mounting cylinder 4, and the side walls of the support plate 9 are vertically slidably connected to the immersion indicator block 31, and a positioning pin 32 is provided between the immersion indicator block 31 and the side wall of the support plate 9.
[0028] By setting a timing scale, the immersion time can be adjusted according to the testing requirements of different test strips. The immersion indicator block 31 is slidable according to the testing requirements of different test strips and locked by the positioning pin 32. The immersion indicator block 31 clearly indicates the immersion length of the test strip, which can further improve the detection accuracy.
[0029] Furthermore, the second cylinder end is integrally formed with a plurality of wedge-shaped clamping blocks 33 that fit against the adjusting shaft 20, and the outer wall of the clamping block 33 is threaded with a fastening nut 34.
[0030] By rotating the fastening nut 34 in the forward direction, the fastening nut 34 presses against the wedge-shaped surface of the clamping block 33, increasing the friction between the clamping block 33 and the adjusting shaft 20, thus fixing the adjusting shaft 20. By rotating the fastening nut 34 in the reverse direction, the friction between the clamping block 33 and the adjusting shaft 20 is reduced, thereby releasing the movement of the adjusting shaft 20.
[0031] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made to it in form and detail without departing from the scope defined by the claims of the present invention.
Claims
1. An immunoassay device that combines sampling and immersion functions, characterized in that: The device includes a base, a placement ring at the bottom of the base, and a disposable sampling cup placed inside the placement ring. An installation cylinder is mounted on the base, and an immersion rod is vertically slidably mounted at the top of the installation cylinder, facing the placement ring. A timed reset device is provided between the immersion rod and the installation cylinder. A test strip is mounted on the immersion rod, and a replacement structure for replacing the test strip is provided inside the installation cylinder. A through groove is opened at the bottom of the installation cylinder, facing the immersion rod. An observation window for observing changes in the test strip is opened on the side wall of the installation cylinder.
2. An immunoassay device with both sampling and immersion functions according to claim 1, characterized in that: The replacement structure includes a rotating shaft coaxially rotatably connected to the bottom of the mounting cylinder. Several horizontal bars are distributed in a circular array around the rotating shaft. A support plate is vertically slidably connected to the end of each horizontal bar. The side of the support plate facing the inner wall of the mounting cylinder is used to place the test strip. A support rod is provided on the side wall of the support plate. Each support rod is rotatably connected to a pressing block for pressing down the test strip, and a torsion spring is provided at the connection. Limiting grooves are opened on both sides of the top of the support plate. A groove is opened at the bottom of the immersion rod. Limiting rods facing the limiting grooves are slidably arranged on both sides of the groove. The ends of the limiting rods are arc-shaped, and elastic reset members are provided between the limiting rods and the grooves.
3. An immunoassay device with both sampling and immersion functions according to claim 2, characterized in that: The positioning and resetting structure includes a resetting rod and a positioning rod fixed to the immersion rod, both of which are L-shaped. A first hydraulic cylinder, fixedly connected to the mounting cylinder, is coaxially sleeved on the vertical section of the resetting rod. A hydraulic plate, connected to the end of the resetting rod, is slidably connected inside the first hydraulic cylinder. The hydraulic plate has an overflow hole, and an elastic resetting element is also provided between the hydraulic plate and the first hydraulic cylinder. A toothed plate is fixed on the vertical section of the positioning rod. A horizontally oriented second hydraulic cylinder passes through the mounting cylinder. An adjusting shaft is slidably connected coaxially inside the second hydraulic cylinder, and a pressure plate, coaxially fixed to the adjusting shaft, is slidably connected inside the second hydraulic cylinder. An overflow hole is also provided on the plate. An elastic reset component is also provided between the adjusting shaft and the second hydraulic cylinder. One end of the adjusting shaft extends out of the mounting cylinder. An annular groove is provided on the circumference of the other end of the adjusting shaft. Several guide grooves are provided on the side of the annular groove away from the mounting cylinder. An annular block is rotatably and slidably connected to the other end of the adjusting shaft. Several guide blocks are provided on the inner wall of the annular block and slidably connected to the guide grooves. Two stops for clamping the annular block are provided on the inner wall of the mounting cylinder. Several pawls that mesh with the toothed plate are hinged on the outer wall of the annular block, and a torsion spring is provided at the connection. One-way limiting blocks that are fixedly connected to the annular block are fitted to the outer arc surface of the pawls.
4. An immunoassay device with both sampling and immersion functions according to claim 3, characterized in that: The rotating shaft passes through the mounting cylinder and is rotatably connected to it, and a rotating handle is fixed at the top of the rotating shaft.
5. An immunoassay device with both sampling and immersion functions according to claim 4, characterized in that: The side wall of the mounting cylinder is provided with a window for replacing the test strip.
6. An immunoassay device with both sampling and immersion functions according to claim 5, characterized in that: The end of the adjusting shaft extending out of the mounting cylinder is provided with timing scale lines, and the side walls of the support plate are vertically slidably connected to immersion indicator blocks. A positioning pin is provided between the immersion indicator block and the side wall of the support plate.
7. An immunoassay device with both sampling and immersion functions according to claim 6, characterized in that: The second cylinder end is provided with several wedge-shaped clamping blocks that fit with the adjusting shaft, and the outer wall of the clamping blocks is threaded with fastening nuts.