Method for preparing high-activity biomass masterbatch by hydrogen-rich water pretreatment coupled with air separation nitrogen production steam explosion and application thereof

Abstract

This invention relates to a method for preparing highly active biomass masterbatches using an air separation nitrogen generation coupled with electrolytic hydrogen-rich water biomass extraction device, and its application, belonging to the field of high-value biomass extraction technology. Based on an integrated extraction device, this invention designs a dual-path differentiated extraction process according to the differences in the thermosensitivity of the raw materials: for non-thermally sensitive / highly lignified raw materials, a path of "low-temperature pretreatment with hydrogen-rich water → high-temperature steam explosion cell disruption → rapid nitrogen cooling → low-temperature high-pressure extraction with hydrogen-rich water and nitrogen" is used; for thermosensitive raw materials, a path B of "low-temperature leaching with hydrogen-rich water → low-temperature high-pressure extraction with nitrogen" is used. The entire process achieves self-production of nitrogen and hydrogen-rich water, eliminating the need for external gas and water sources. This invention can scalably prepare various highly active biomass masterbatches such as willow tea thearubigin, whole tea polyphenols / theanine / catechins, corn germ SOD three enzymes, ginsenosides, and bird's nest peptides, with a masterbatch activity retention rate ≥98% and a significantly improved yield; simultaneously, it protects the masterbatch and its applications in food, health products, and cosmetics.

Description

Technical Field

[0001] This invention relates to the field of efficient extraction and high-value utilization of biomass resources, specifically to a method for preparing highly active biomass masterbatch by coupling hydrogen-rich water pretreatment with air separation nitrogen production steam explosion and its application, and particularly to the application of using self-produced hydrogen-rich water as a solvent to compound biomass masterbatch and peptide active ingredients to prepare functional cosmetics. Background Technology

[0002] Existing biomass active ingredient extraction technologies suffer from problems such as limited equipment functionality and low process integration. High-temperature cell disruption can easily damage heat-sensitive components, while low-temperature extraction results in low cell disruption efficiency and poor yield. Furthermore, gas sources and hydrogen-rich water are often purchased or prepared separately, leading to fragmented processes and easy oxidation and deactivation of active ingredients during material transfer.

[0003] Meanwhile, existing processes lack differentiated designs for the characteristics of different raw materials, making it impossible to achieve strong cell wall breaking of highly lignified raw materials and low-temperature protection of heat-sensitive raw materials on the same set of equipment, resulting in low equipment utilization; moreover, most of the extracted active ingredients have not formed standardized masterbatches, and there is a lack of clear proportions and effect support for end applications. Summary of the Invention

[0004] The purpose of this invention is to provide a dual-path biomass extraction process relying on a specific integrated extraction device to achieve efficient and differentiated extraction of non-heat-sensitive / high-lignification raw materials and heat-sensitive raw materials, thereby maximizing the protection of active ingredients; at the same time, it produces standardized high-activity biomass masterbatch and clarifies its specific applications in food, health products, and cosmetics, thus constructing a complete innovation chain of "equipment-process-product-application", with specific process parameters and the ability to be mass-produced.

[0005] This invention is achieved through the following technical solution: In a first aspect, this invention provides a method for preparing highly active biomass masterbatch by coupling hydrogen-rich water pretreatment with air separation nitrogen production steam explosion. The core of this method is a dual-path differentiated extraction process, and the specific technical solution is as follows: (1) General pretreatment of raw materials Select biomass raw materials that are free from mold and impurities, coarsely wash them using the tap water branch of the device, and pulverize them to 40-80 mesh (40-60 mesh for lignified raw materials and 60-80 mesh for heat-sensitive raw materials) according to the characteristics of the raw materials. Dry them at a low temperature of 40-50℃ to a moisture content of 10-15% (drying can be omitted for heat-sensitive raw materials, and fresh materials can be used directly) for later use.

[0006] (2) and any of the following extraction paths: Path A (suitable for non-heat-sensitive / highly lignified raw materials) Suitable for willow tea, various teas, corn germ, ginseng and other highly lignified / non-heat-sensitive raw materials, the core process is hydrogen-rich water pretreatment + steam explosion cell disruption + nitrogen protection throughout the process, the steps are as follows: S1. Low-temperature long-term immersion pretreatment with hydrogen-rich water: The pretreated raw material is put into the coupling extraction host tank and injected with 800-1200 ppb hydrogen-rich water produced by the device. The liquid-to-material ratio is 5:1~8:1, the tank pressure is 0.3~0.5MPa, the immersion temperature is 4~10℃, and the immersion time is 24~36h. The hydrogen-rich water is used only as an antioxidant protective medium to penetrate the raw material and inhibit the oxidation of components. S2. High-temperature steam explosion core cell wall breaking: discharge hydrogen-rich water (which can be recycled), close the tank outlet, introduce high-temperature and high-pressure steam, control the explosion pressure at 1.0~1.2MPa and the explosion temperature at 180~200℃, hold the pressure for 10~30s and then release the pressure instantly to completely break the plant cell wall. S3. Rapid nitrogen cooling and oxidation prevention: Immediately introduce high-pressure nitrogen produced by the device, with an inlet pressure of 0.5~0.8MPa. The temperature of the tank will be reduced to below 40℃ within 1~3 minutes through the dual-chamber heat transfer oil cooling chamber, terminating the thermal reaction. The nitrogen isolates the components from the air to prevent oxidation after the cell wall is broken. S4. Hydrogen-rich water + nitrogen low-temperature high-pressure extraction: Maintain a slight positive pressure of nitrogen in the tank (0.1~0.2MPa), inject the self-produced RO pure water, with a liquid-to-material ratio of 10:1~15:1, turn on the low-speed shear component (20~40r / min) at 35~45℃, and extract for 2~3h; For raw materials requiring enzymatic hydrolysis (such as corn germ, dietary fiber raw materials), appropriate biological enzymes can be added before extraction (addition amount is 0.5~1.0% of the dry weight of the raw material), enzymatic hydrolysis at 30~40℃ for 4~6h, and then extraction, with nitrogen protection throughout the process; S5. Post-processing of masterbatch preparation: After extraction, the discharged liquid is separated into solid and liquid components by plate and frame filtration / centrifugation. The crude extract is purified by microfiltration + ultrafiltration membrane separation, concentrated at ≤50℃ under vacuum, and spray-dried / freeze-dried to obtain standardized powdered biomass masterbatch, which is then sealed and stored away from light.

[0007] Pathway B (suitable for heat-sensitive raw materials) Suitable for heat-sensitive / easily oxidized raw materials such as bird's nest, agarwood, fresh plant juices, and marine algae. The entire process is kept at a low temperature to prevent high-temperature explosions. The steps are as follows: S1. Low-temperature extraction with hydrogen-rich water: The pretreated raw materials (fresh materials / dried powder) are put into the coupling extraction host tank and injected with 800-1200 ppb hydrogen-rich water produced by the device. For heat-sensitive animal and plant raw materials, food-grade extraction aids can be added (such as adding anhydrous ethanol to agarwood and adding neutral protease to bird's nest). The liquid-to-material ratio is 8:1~12:1, the tank pressure is 0.2~0.4MPa, the extraction temperature is ≤25℃, and the extraction is carried out at low speed (10~20r / min) for 3~4h. The hydrogen-rich water provides antioxidant protection throughout the process. S2. Nitrogen low-temperature high-pressure extraction: High-pressure nitrogen generated by the device is introduced into the tank, and the extraction pressure is controlled at 0.4~0.8MPa and the extraction temperature at 20~25℃. The nitrogen is maintained at a slightly positive pressure for 3~5 hours, and the temperature is controlled at ≤25℃ throughout the process to avoid damage to the active ingredients. S3. Post-processing of masterbatch preparation: After extraction, the pressure is reduced (slowly depressurized to prevent component deactivation), and the liquid is filtered at low temperature (≤25℃), purified by membrane separation, and concentrated at low temperature under vacuum (≤40℃) to obtain standardized biomass masterbatch in liquid / paste / powder form, which is then sealed and stored at low temperature (4~10℃). After the masterbatch post-processing, for heat-sensitive or acid-sensitive raw materials, it is necessary to further encapsulate them with one or more nanoliposomes to improve their stability.

[0008] The extraction path is achieved using an air separation nitrogen generation coupled with electrolytic hydrogen-rich water biomass extraction device. The device produces high-pressure nitrogen with a purity ≥99.999%, hydrogen-rich water with a purity of 800-1200 ppb, and RO pure water with a conductivity ≤10 μS / cm.

[0009] Secondly, the present invention provides highly active biomass masterbatches prepared by the above-mentioned path A or path B, including but not limited to: willow tea melanin masterbatch, whole tea polyphenol / theanine / catechin composite masterbatch, corn germ SOD three-enzyme masterbatch, five-year-old ginsenoside masterbatch, and bird's nest peptide masterbatch. The masterbatch has an active ingredient retention rate of ≥98%, an impurity content of ≤5%, and stable quality, and can be directly used as a core raw material in the production of food, health products, and cosmetics.

[0010] The corn germ SOD three-enzyme masterbatch of the present invention is prepared by adding cellulase + papain compound enzymatic hydrolysis, with SOD activity ≥10000U / g, and CAT and POD enzyme activities ≥500U / g; the five-year-old ginsenoside masterbatch has a total saponin content ≥85% and Rg1+Re+Rb1 content ≥30%.

[0011] This invention provides a highly active biomass masterbatch, prepared via the above-mentioned pathway A or pathway B, comprising one or more of the following: willow tea melanin masterbatch, whole tea polyphenol / theanine / catechin composite masterbatch, corn germ SOD three-enzyme masterbatch, five-year-old ginsenoside masterbatch, and bird's nest peptide masterbatch.

[0012] Thirdly, this invention provides applications for the highly active biomass masterbatch prepared via path A or path B, which can be used alone or in combination in the preparation of food, health food, and cosmetics. Core applications include calming the nerves and aiding sleep, anti-oxidation, enhancing immunity, and regulating metabolism. In specific applications, it can be combined with food / cosmetic grade matrices, homogenized, granulated, and filled according to a certain ratio. The compounded masterbatch has a synergistic effect. The device of this invention produces hydrogen-rich water, which can be directly used as a solvent and carrier of active ingredients in functional cosmetics. It can be compounded with the highly active biomass masterbatch and peptide active ingredients such as nonapeptide-1 obtained by this invention to prepare anti-aging, whitening, and repairing functional cosmetics. The strong antioxidant properties of hydrogen-rich water can improve the stability and skin penetration efficiency of active ingredients in cosmetics, while reducing the skin irritation of peptides, niacinamide, and other ingredients. It is suitable for various skin types, including sensitive skin and early-mature skin. After compounding, hydrogen-rich water, biomass masterbatch, and peptide active ingredients form a synergistic effect, significantly improving the anti-aging, whitening, and repairing effects of cosmetics.

[0013] The cosmetic product described in this invention is a calming and sleep-aiding fragrance, prepared from the following weight ratios: 1.0-2.0% theanine masterbatch, 0.5-1.0% agarwood essential oil masterbatch, 0.5-0.8% sandalwood essential oil masterbatch, 1.5-2.5% SOD triple enzyme masterbatch, and 94-96% cosmetic-grade slow-release matrix.

[0014] The health food product of this invention is an antioxidant solid beverage, prepared from the following weight ratios: 4-6% willow tea melanin masterbatch, 7-9% tea polyphenol masterbatch, 8-12% SOD trienzyme masterbatch, 2-4% ginsenoside masterbatch, 25-32% erythritol, 15-18% fructooligosaccharides, 10-12% inulin, 5-8% resistant dextrin, 3-5% maltodextrin, 2-4% blueberry anthocyanins, 0.2-0.5% curcumin, 0.1-0.3% apigenin, 0.1-0.3% sulforaphane, 0.05-0.1% allicin, 0.5-1% garland chrysanthemum extract, 1-2% creatine, 0.05-0.1% ergothioneine, and 0.1-0.3% polydeoxynucleotides.

[0015] This invention uses 800-1200ppb hydrogen-rich water produced by the device as a solvent / active carrier, and combines it with highly active biomass masterbatch and peptide active ingredients to prepare functional cosmetics. The hydrogen-rich water is prepared by the electrolytic hydrogen-rich water preparation branch of the device and has a conductivity ≤10μS / cm.

[0016] The peptide active ingredients of this invention are one or more of nonapeptide-1, hexapeptide-8, and copper peptide. The functional cosmetics include, but are not limited to, anti-aging essence, whitening and spot-fading cream, sensitive skin repair ampoule, toner, and lotion. The mass ratio of hydrogen-rich water, biomass masterbatch, and peptide active ingredients is (80-95):(3-15):(0.01-0.5).

[0017] The functional cosmetics of the present invention may also contain one or more auxiliary active ingredients selected from sodium hyaluronate, ceramide NP, plant extracts, and niacinamide, with the amount of auxiliary active ingredients added being 0.1-5% of the total mass of the cosmetics.

[0018] This invention utilizes high-concentration negative hydrogen-rich water prepared by cathode preparation for pre-immersion and permeation treatment of plant raw materials within a sealed pressure tank, rather than ordinary washing or dissolution. Through the strong penetrability of highly active hydrogen molecules in the hydrogen-rich water, these molecules directionally penetrate plant cell walls, loosening the cell structure and enhancing the release efficiency and extraction rate of active ingredients. This high-concentration hydrogen-rich water is then used as process water for enzymatic hydrolysis and fermentation, maintaining an effective concentration of hydrogen molecules throughout the process and ensuring process stability. Unlike ordinary alkaline water or low-concentration hydrogen water, this invention truly achieves efficient, non-destructive, and high-rate ecological extraction of plant active ingredients.

[0019] This invention discloses a fully ecological, solvent-free, and highly active plant extract process, belonging to the field of natural plant extraction technology. This process employs a sealed, pressurized, high-concentration, negative-hydrogen-rich water pre-immersion treatment of plant raw materials. Utilizing the strong penetrability of hydrogen molecules, it loosens and pre-breaks plant cell walls, significantly improving the release efficiency and extraction rate of active ingredients. Subsequently, it combines high-pressure, low-nitrogen, and low-temperature synergistic extraction technology (airborne extraction) with physical steam explosion technology. The entire process uses no organic solvents such as ether or alcohols, and no chemical additives are used. Cell wall breaking, component release, enzymatic hydrolysis, and fermentation are completed solely through physical methods, achieving non-destructive extraction of plant active ingredients. This process maintains a stable hydrogen concentration through a sealed pressurized tank, unlike ordinary alkaline water or low-concentration hydrogen water treatment methods. It truly forms a green, ecological, closed-loop, and highly active industrial production system, suitable for the efficient extraction of various natural raw materials such as tea, medicinal and edible plants, grain germ, fruits and vegetables, and marine fungi. It boasts advantages such as high extraction rate, complete preservation of activity, no chemical residues, and environmental friendliness.

[0020] Fourthly, this invention provides an air separation nitrogen generation coupled with electrolytic hydrogen-rich water biomass extraction device for realizing the extraction path. The device includes a pre-air nitrogen generation system, a three-way parallel intelligent water supply system, a front-end fully automatic raw material pretreatment unit, a low-temperature enzymatic hydrolysis and low-temperature fermentation pretreatment unit, a nitrogen vapor explosion coupled core extraction host, a high-pressure nitrogen low-temperature extraction device, a dual-mode solid-liquid separation unit, a closed-loop wastewater purification and reuse unit, a residue recovery and resource utilization unit, a back-end purification, freeze-drying, and automatic packaging unit, a CIP 360° fully automatic cleaning unit, a cGMP workshop environmental control unit, and a fully sensor-linked manual / automatic dual-mode control system; each unit is connected to a DN25-DN80... The food-grade 304 / 316L stainless steel quick-connect sealing pipe is physically and seamlessly connected to the intelligent electric quick-connect ball valve. The three-way parallel intelligent water supply system is equipped with an electrolytic hydrogen-rich water preparation branch. This branch is configured with a dedicated hydrogen-rich water dual-inlet and three-outlet pipeline structure. The dedicated hydrogen-rich water dual-inlet and three-outlet pipeline structure is made of food-grade 316L stainless steel. The electrolysis module electrode adopts one of platinum, titanium, titanium alloy or platinum-titanium composite electrode. The nitrogen vapor explosion coupling core extraction host is equipped with a dual-tank hot and cold oil circulation temperature control system and a triple cooling linkage component. The entire system is equipped with high-precision sensors.

[0021] Furthermore, the dedicated hydrogen-rich water dual-inlet and triple-outlet pipeline structure includes dual-input pipelines sealed to the cathode outlet of the electrolysis module, and a triple-outlet intelligent control valve group merging with the dual-input pipelines. The dual-input pipelines are in a parallel structure with one main and one backup, each equipped with a flow sensor, check valve, and electric shut-off valve. Electrical linkage with the control system enables automatic fault switching and triggers audible and visual alarms. The fault determination condition is that the pipeline flow rate is lower than the set value of 0.1 m³ / h. Or pressure fluctuations exceeding ±0.05MPa; the three-outlet intelligent control valve group has three independent outlets, which are respectively sealed to the hydrogen-rich water special process water inlet of the hydrogen explosion tank, enzymatic hydrolysis tank, and fermentation tank. Each outlet is equipped with an independent electric regulating valve, pressure sensor, and flow recorder, which can realize independent control of feeding pressure 0.2-0.5MPa and flow rate 0-5m³ / h, and support single / multi-channel simultaneous feeding; the electrolytic hydrogen-rich water preparation branch is equipped with a dual water source supply structure, which can be connected to ion exchange water or RO reverse osmosis pure water. After electrolysis, there are cathode hydrogen-rich water storage tanks and anode oxygen-rich water storage tanks. The cathode hydrogen-rich water storage tank is connected to a special hydrogen storage tank. The hydrogen storage tank delivers hydrogen to each hydrogen-using unit at regular intervals through a liquid level control system. The anode oxygen-rich water storage tank is a closed structure and equipped with a control system. The output oxygen-rich water is used for front-end cleaning and sterilization of equipment and CIP sterilization cleaning.

[0022] Furthermore, the pre-air nitrogen generation system is a three-stage filtration-deep purification-high-pressure low-temperature air separation full-process structure, which includes, in sequence, a three-stage physical isolation filter layer with differential pressure sensor, a deep purification and sterilization component, an air compressor unit, a high-pressure low-temperature air separation unit, a nitrogen purification and pressurization component, and a by-product recovery component. The filtration accuracy of the three-stage physical isolation filter layer is ≥5μm, ≥1μm, and ≥0.3μm, respectively. The high-purity nitrogen produced by the system has a purity of ≥99.999%, and is output in multiple channels to the enzymatic hydrolysis tank, hydrogen explosion tank, front-end pretreatment unit soaking tank, and high-pressure nitrogen low-temperature extraction device, providing nitrogen inert protection and extraction gas source. The by-product oxygen is purified and recovered to oxygen cylinders for sale. The by-product water vapor is condensed and collected as clean water, which is returned to the tap water input of the three-way parallel intelligent water supply system for further treatment and reuse.

[0023] Furthermore, the nitrogen vapor explosion coupling core extraction host is a food-grade stainless steel pressure-resistant tank. The outer wall of the tank is equipped with a single-layer heat-conducting oil temperature control jacket, and adopts a design of cold oil entering from the top and hot oil returning from the bottom by gravity. It is equipped with dual independent sealed oil tanks for hot and cold oil and an electric three-way switching valve. The tank is equipped with pressure / temperature sensors, which can trigger a triple cooling linkage component to achieve rapid cooling. The triple automatic cooling reduces the tank temperature to below 40℃ within 1-3 minutes. The temperature control system of the dual oil tank cold and hot oil circulation has a temperature control range of 25-180℃ and a temperature control accuracy of ±0.5℃. The tank is also equipped with a staged cell wall breaking control module, which can switch between two modes: pure nitrogen high-pressure cell wall breaking and steam explosion nitrogen full-process protection. The tank is equipped with a shearing and crushing component, an ultrasonic-assisted cell wall breaking system and a sanitary scraping component. The top is equipped with a 360° spray cleaning ball and a safety pressure relief device.

[0024] Furthermore, the low-temperature enzymatic hydrolysis and low-temperature fermentation pretreatment unit includes an enzymatic hydrolysis tank and a fermentation tank. Both tanks are equipped with a temperature control jacket and a dedicated hydrogen-rich water inlet. The temperature control jacket controls the temperature at 25-40℃ with an accuracy of ±0.5℃ and is covered with a 50mm polyurethane insulation layer. The enzymatic hydrolysis tank is a closed pressure-bearing structure, equipped with a variable frequency speed-regulating stirring assembly, an enzymatic hydrolysis feed module, and a nitrogen inlet. The fermentation tank is a closed atmospheric pressure structure, equipped with a sterile sampling port, a sterile exhaust device with a hydrophobic membrane and activated carbon filter, and a microbial inoculation module. Both tanks are equipped with multiple types of sensors for temperature, pH, and dissolved oxygen, and are also equipped with 360° spray cleaning balls.

[0025] Furthermore, the front-end fully automated pre-processing unit for raw materials is equipped with dedicated processing sub-modules for tea and calming / sleep-aiding raw materials. This sub-module includes a dedicated soaking tank for electrolytic hydrogen-rich water, an automatic tea stem separation device, and a grading and crushing component for calming / sleep-aiding raw materials. The dedicated soaking tank is made of food-grade 316L stainless steel and is equipped with a temperature control jacket, a nitrogen inlet, a low-speed stirring paddle, and multiple sensors for liquid level, temperature, and pH. The temperature of the electrolytic hydrogen-rich water soaking is controlled at 20-30℃. The grading and crushing component is adapted to meet the differentiated low-loss crushing requirements of hard raw materials such as agarwood and sandalwood, and gentle crushing of herbal raw materials such as spikenard and vetiver.

[0026] Furthermore, the high-pressure nitrogen cryogenic extraction device is connected to the discharge end of the nitrogen vapor explosion coupling core extraction host, and adopts a low-temperature high-pressure nitrogen extraction process with an extraction pressure of 0.5-1.2MPa and an extraction temperature of 20-40℃. It is electrically connected to a fully sensor-linked manual and automatic dual-mode control system to achieve precise control of extraction parameters.

[0027] Furthermore, the dual-mode solid-liquid separation unit adopts a parallel structure of a plate and frame filter press and a centrifugal dewatering machine, both made of food-grade stainless steel, equipped with a material conveying pump and a liquid level / pressure sensor. It can automatically / manually switch the separation mode according to the material characteristics and is equipped with an online cleaning system. The extract liquid of tea and calming and sleep aid raw materials is suitable for the 3000-4000r / min low-speed centrifugal dewatering mode. The closed-loop wastewater purification and reuse unit is a full-process closed-loop structure. It can treat all wastewater from the production line through "grid filtration → equalization tank → anaerobic reaction tank → aerobic reaction tank → sedimentation tank → sand filtration → carbon filtration → nanofiltration → activated carbon adsorption → ultraviolet sterilization" and then recycle it, achieving zero wastewater discharge.

[0028] Furthermore, the fully sensor-linked manual / automatic dual-mode control system is based on a PLC + touch screen + industrial computer, and includes a sensor network layer, an actuator network layer, and a core control layer. It also adds a dedicated control module for hydrogen-rich water pipelines, a triple cooling linkage control module, and dedicated process formula modules for tea and calming / sleep-aiding raw materials. This system can be linked with all production line equipment, sensors, and valves, supports automatic / manual mode switching, and has safety interlock protection, remote monitoring, and parameter recording and traceability functions.

[0029] Furthermore, the CIP 360° fully automatic cleaning unit is sealed to all tanks, equipment, and pipelines of the production line, enabling fully automated cleaning of clean water, acid, alkali, rinsing, neutralization, and drying. The oxidizing water generated by the anode of the electrolysis module can be connected to this unit as sterilization and cleaning water, and the cleaning waste liquid is connected to the closed-loop wastewater purification and reuse unit. The cGMP workshop environmental control unit can accurately control the workshop temperature at 25±5℃, humidity at 40-60% RH, and cleanliness level D and above. The waste gas treatment system is equipped with a low-temperature essential oil recovery module.

[0030] The device can produce high-pressure nitrogen with a purity ≥99.999%, hydrogen-rich water with a purity of 800-1200ppb, and RO pure water with a conductivity ≤10μS / cm. The coupling extraction host is a vertical 316L stainless steel pressure tank with multiple interfaces for steam / nitrogen / hydrogen-rich water / pure water, a dual-chamber heat transfer oil jacket (rapid cooling in 1-3 minutes), 360° cleaning, and low-speed shearing.

[0031] The device is equipped with a pre-air nitrogen generation system consisting of three-stage filtration, deep purification, and high-pressure low-temperature air separation; a dual-tank hot and cold oil circulation temperature control system; and triple automatic cooling technology. It also features a dedicated soaking module for electrolytic hydrogen-rich water, making it suitable for the green pretreatment and efficient extraction of calming and sleep-aiding raw materials such as tea, agarwood / sandalwood. The device achieves nitrogen self-sufficiency, zero wastewater discharge, and fully unmanned operation.

[0032] Compared with the prior art, the present invention has the following advantages and beneficial effects: 1. This invention is based on the same integrated extraction device and designs a differentiated dual-path process to adapt to two types of raw materials: non-heat-sensitive / high-lignification and heat-sensitive materials. It solves the problem of the single function of traditional equipment and greatly improves equipment utilization and production flexibility. 2. The present invention uses “hydrogen-rich water pretreatment + rapid cooling after explosion + nitrogen protection throughout the process” in path A, and “low-temperature hydrogen-rich water + nitrogen protection throughout the process” in path B. The dual anti-oxidation methods make the retention rate of active ingredients ≥98%, and the steam explosion cell wall breaking increases the extraction yield by more than 30%, which is far superior to the traditional process. 3. This invention enables the self-production of nitrogen, hydrogen-rich water, and RO pure water using a single device, eliminating the need for external purchases and reducing production and transportation costs. Material cell disruption, extraction, and cooling are all completed in the same tank, with no material transfer, avoiding secondary oxidation. The processes are tightly integrated, and the hydrogen-rich water can be recycled, while the byproduct oxygen can be reused industrially, making it green and environmentally friendly. 4. The biomass masterbatch obtained by this invention is a standardized product with stable quality and can be used directly as a core raw material; and different masterbatches have synergistic effects when compounded (such as theanine + agarwood essential oil for sleep aid, SOD three enzymes + tea polyphenols for antioxidant effects), making it suitable for the production of high-end end products. 5. The process of this invention is entirely based on prior utility model patent equipment, with specific parameters, experimental verification, no complex algorithms, and full disclosure; all steps are based on mature industrial modules, which can be directly mass-produced, and at the same time, a complete protection chain of "equipment-process-product-application" is constructed. 6. The device of this invention is a self-sufficient air-to-nitrogen generator with a purity of 99.999%, which reduces costs and ensures supply; 7. The device of this invention enables graded cell wall disruption of both heat-sensitive and non-heat-sensitive raw materials, balancing activity retention and extraction efficiency; 8. The device of this invention is designed with a dedicated dual-tank hot and cold oil circulation temperature control system, combined with steam explosion triple automatic cooling, which provides precise temperature control and efficient cooling. 9. The device of this invention adopts low-temperature enzymatic hydrolysis + static fermentation pretreatment to reduce damage to heat-sensitive components and lower operating costs; 10. The device of this invention is designed with a special pipeline structure of dual-input hydrogen-rich water pipelines and three-outlet intelligent control valve groups to achieve precise and automated delivery of hydrogen-rich water to hydrogen explosion tanks, enzymatic hydrolysis tanks and fermentation tanks; 11. The device of this invention realizes graded utilization of water resources, zero discharge of wastewater, and resource utilization of residue, which meets the requirements of green production; 12. The device of the present invention improves accuracy and efficiency through full-process sensing linkage and unmanned operation, and meets the requirements of cGMP data traceability and production. 13. The device of the present invention is equipped with a special soaking module for electrolyzing hydrogen-rich water, which realizes green pretreatment of raw materials such as tea, agarwood / sandalwood / nard / vetiver, etc., which are used to calm the nerves and aid sleep, removes pesticide residues and heavy metals without chemical residues, and realizes graded resource utilization of electrolyzed water at the cathode and anode, thereby improving the water resource utilization rate. 14. This invention is suitable for cell wall breaking and extraction of active ingredients from tea, calming and sleep-aiding products, and other biomass raw materials, improving the extraction and retention rates of tea polyphenols, theanine, plant essential oils, calming terpenes, and other components, meeting the raw material production standards for high-end daily chemical products such as calming and sleep-aiding cosmetics, perfumes, and single-use ampoules. Attached Figure Description

[0033] The accompanying drawings, which are included to provide a further understanding of embodiments of the invention and form part of this application, do not constitute a limitation thereof. In the drawings: Figure 1 Main diagram showing the overall process flow and connection of core equipment in the production line; Figure 2 Schematic diagram of a dedicated pipeline structure for hydrogen-rich water with two inlets and three outlets; Figure 3 A schematic diagram of the structure of the nitrogen vapor explosion coupling core extraction host; Figure 4 This is a schematic diagram of the low-temperature enzymatic hydrolysis and low-temperature fermentation pretreatment unit. Figure 5 This is a schematic diagram of a three-way parallel intelligent water supply system; Figure 6 This is a schematic diagram showing the connection structure between the full-sensor linkage control system architecture and the hydrogen-rich water pipeline control module.

[0034] The attached diagram shows the markings and corresponding component names: 1-Pre-air nitrogen generation system, 101-Three-stage physical isolation filter layer, 102-Byproduct recovery component, 2-Three-way parallel intelligent water supply system, 21-Electrolysis module, 212-Cathode hydrogen-rich water storage tank, 213-Anode oxygen-rich water storage tank, 214-Hydrogen storage tank, 22-Dual hydrogen-rich water input pipeline, 221-Main input pipeline, 222-Backup input pipeline, 223-Flow sensor, 224-Check valve, 225-Electric shut-off valve, 23 - Three-outlet intelligent control valve assembly, 231-Solenoid directional valve, 232-Pressure regulator, 233-Flow metering module, 234-Hydrogen explosion tank outlet, 235-Enzymatic hydrolysis tank outlet, 236-Fermentation tank outlet, 237-Electric regulating valve, 238-Pressure sensor, 3-Front-end fully automatic raw material pretreatment unit, 4-Low-temperature enzymatic hydrolysis and low-temperature fermentation pretreatment unit, 41-Enzymatic hydrolysis tank, 412-Nitrogen inlet, 413-Hydrogen-rich water special process water inlet 414-Temperature control sensor, 415-Variable frequency speed regulating stirring assembly, 416-Temperature control jacket, 417-Material outlet, 42-Fermentation tank, 422-Upper cover flange assembly II, 423-Aseptic exhaust device, 424-pH sensor, 425-Temperature control jacket, 426-Fermentation material outlet, 5-Nitrogen vapor explosion coupling core extraction host, 501-Food grade stainless steel pressure-resistant tank body, 502-Single-layer heat transfer oil temperature control jacket, 503 - Independent sealed oil tanks for hot and cold oil, 504- Triple cooling linkage component, 6- High-pressure nitrogen low-temperature extraction device, 7- Dual-mode solid-liquid separation unit, 8- Closed-loop wastewater purification and reuse unit, 9- Residue recovery and resource utilization unit, 10- Back-end purification freeze-drying automatic packaging unit, 11- CIP 360° fully automatic cleaning unit, 12- cGMP workshop environmental control unit, 13- Fully sensor-linked manual and automatic dual-mode control system. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments and accompanying drawings. The illustrative embodiments and descriptions of this invention are for explanation only and are not intended to limit the invention. All embodiments of this invention rely on an air separation nitrogen production coupled with electrolytic hydrogen-rich water biomass extraction device. The device produces nitrogen with a purity ≥99.999%, hydrogen-rich water with a purity of 800-1200 ppb, and RO pure water with a conductivity ≤10 μS / cm.

[0036] Example 1 Example 1 provides the preparation of willow tea melanin masterbatch (Path A: Non-thermal sensitive / highly lignified raw material). Raw material pretreatment: Select fresh leaves of three-year-old willow tea, coarsely wash them with tap water from the device, crush them to 50 mesh, and dry them at a low temperature of 45℃ to a moisture content of 12% for later use; Low-temperature soaking pretreatment with hydrogen-rich water: Take 1 kg of dried willow tea powder and put it into the coupling extraction host tank, inject 1000 ppb of hydrogen-rich water, liquid-to-material ratio 6:1, tank pressure 0.4 MPa, soaking temperature 8℃, soaking for 30 h. Steam explosion cell disruption: Hydrogen-rich water is discharged (recovered and recycled), high-temperature and high-pressure steam is introduced, the explosion pressure is controlled at 1.1 MPa and the explosion temperature at 190℃, and the pressure is maintained for 20 seconds before instantaneous pressure release; Nitrogen rapid cooling: Immediately introduce high-pressure nitrogen at an inlet pressure of 0.6 MPa, and the tank temperature will drop to 38°C within 1.5 minutes; Low-temperature high-pressure extraction: Maintain a slight positive pressure of nitrogen at 0.15 MPa, inject RO pure water, liquid-to-solid ratio of 12:1, low-speed shearing at 40℃ for 30 r / min, extraction for 2.5 h; Post-processing of masterbatch: The liquid is separated into solid and liquid by centrifugation, the crude extract is purified by microfiltration and ultrafiltration, concentrated at 45℃ under vacuum, and freeze-dried to obtain tea melanin masterbatch powder. Testing indicators: the content of tea black pigment ≥75%, the activity retention rate 98.8%, and the impurity content ≤3%.

[0037] Example 2 Example 2 provides the preparation of a whole tea polyphenol / theanine / catechin composite masterbatch (Pathway A: Non-thermal sensitive / highly lignified raw material). Raw material pretreatment: Select a mixture of green tea, black tea and oolong tea (ratio 1:1:1), coarsely wash with tap water from the device, grind to 60 mesh, dry at 40℃ to 10% moisture content, and set aside. Low-temperature soaking pretreatment with hydrogen-rich water: Take 1 kg of mixed tea powder and put it into the tank, inject 1200 ppb of hydrogen-rich water, liquid-to-material ratio 7:1, tank pressure 0.5 MPa, soaking temperature 6℃, soaking for 36 h. Steam explosion cell disruption: Hydrogen-rich water is discharged, high-temperature and high-pressure steam is introduced, the explosion pressure is controlled at 1.0 MPa and the explosion temperature at 180℃, and the pressure is held for 15 seconds before instantaneous pressure release; Nitrogen rapid cooling: High-pressure nitrogen is introduced at an inlet pressure of 0.5 MPa, and the tank temperature is reduced to 35°C within 1 minute; Low-temperature high-pressure extraction: Maintain a slight positive pressure of nitrogen at 0.1 MPa, inject RO pure water, liquid-to-solid ratio of 15:1, low-speed shearing at 35℃ for 20 r / min, and extract for 3 h; Post-processing of masterbatch: The liquid is filtered through plate and frame filter, the crude extract is purified by microfiltration and ultrafiltration, concentrated at 40℃ under vacuum, and spray-dried to obtain whole tea composite masterbatch powder; Testing indicators: tea polyphenol content ≥45%, theanine ≥8%, catechins ≥20%, total activity retention rate 99.2%.

[0038] Example 3 Example 3 provides the preparation of corn germ SOD three-enzyme masterbatch (Path A: Enzyme-assisted extraction). Raw material pretreatment: Select fresh corn germ, coarsely wash it with tap water from the device, crush it to 40 mesh, and dry it at a low temperature of 50℃ to a moisture content of 15% for later use; Low-temperature soaking pretreatment with hydrogen-rich water: Take 1 kg of corn germ dry powder and put it into the tank, inject 800 ppb of hydrogen-rich water, liquid-to-material ratio 5:1, tank pressure 0.3 MPa, soaking temperature 10℃, soaking for 24 hours. Steam explosion cell disruption: Hydrogen-rich water is discharged, high-temperature and high-pressure steam is introduced, the explosion pressure is controlled at 1.2 MPa and the explosion temperature at 200℃, and the pressure is held for 30 seconds before instantaneous pressure release; Nitrogen rapid cooling: High-pressure nitrogen is introduced at an inlet pressure of 0.8 MPa, and the tank temperature is reduced to 40°C within 2 minutes; Enzymatic hydrolysis + low-temperature high-pressure extraction: Maintain a slight positive pressure of nitrogen at 0.2 MPa, inject RO pure water, liquid-to-solid ratio of 10:1, add cellulase + papain (compound ratio 1:1, total addition 0.8%), enzymatic hydrolysis at 35℃ for 5 h; after enzymatic hydrolysis, extract at 45℃ at low speed of 40 r / min for 2 h. Post-processing of masterbatch: The liquid is separated by low-temperature centrifugation, the crude extract is purified by membrane separation, concentrated at 48℃ under vacuum, and freeze-dried to obtain corn germ SOD three-enzyme masterbatch powder; Detection indicators: SOD activity ≥10000U / g, CAT and POD enzyme activities ≥500U / g, and total activity retention rate of the three enzymes 98.5%.

[0039] Example 4 Example 4 provides the preparation of five-year-old ginsenoside masterbatch (Pathway A: Non-thermal-sensitive / highly lignified raw material). Raw material pretreatment: Select five-year-old ginseng roots, roughly wash them with tap water from the device, slice them, pulverize them to 50 mesh, and dry them at a low temperature of 45℃ to a moisture content of 12% for later use; Low-temperature soaking pretreatment with hydrogen-rich water: Take 1 kg of ginseng powder and put it into the tank, inject 1000 ppb of hydrogen-rich water, liquid-to-material ratio 6:1, tank pressure 0.4 MPa, soaking temperature 8℃, soaking for 30 h. Steam explosion cell disruption: Hydrogen-rich water is discharged, high-temperature and high-pressure steam is introduced, the explosion pressure is controlled at 1.1 MPa and the explosion temperature at 190℃, and the pressure is maintained for 20 seconds before instantaneous pressure release; Nitrogen rapid cooling: High-pressure nitrogen is introduced at an inlet pressure of 0.6 MPa, and the tank temperature is reduced to 38°C within 1.5 minutes; Low-temperature high-pressure extraction: Maintain a slight positive pressure of nitrogen at 0.15 MPa, inject RO pure water, liquid-to-solid ratio of 12:1, low-speed shearing at 40℃ for 30 r / min, extraction for 2.5 h; Post-processing of masterbatch: The liquid is separated by centrifugation, the crude extract is purified by membrane separation, concentrated under vacuum at 45℃, and spray-dried to obtain ginsenoside masterbatch powder; Testing indicators: total ginsenoside content ≥85%, Rg1+Re+Rb1 content ≥30%, and monomeric saponin activity retention rate 99.0%.

[0040] Example 5 Example 5 provides the preparation of bird's nest peptide masterbatch (Pathway B - Thermosensitive Raw Material). Raw material pretreatment: Select dried bird's nest, gently wash it with the tap water branch of the device to remove impurities, then soak it in RO pure water until softened, cut it into small strips, and use the fresh material directly without drying; Low-temperature extraction with hydrogen-rich water: Take 500g of bird's nest strips and put them into the tank. Add 1000ppb of hydrogen-rich water and add neutral protease (addition amount 0.5%) as an extraction aid. The liquid-to-material ratio is 10:1, the tank pressure is 0.3MPa, the extraction temperature is 20℃, the low-speed shearing is 15r / min, and the extraction time is 4h. Nitrogen low-temperature high-pressure extraction: High-pressure nitrogen is introduced into the tank, and the extraction pressure is controlled at 0.6MPa and the extraction temperature at 22℃. The nitrogen is maintained at a slightly positive pressure for 4 hours, and the temperature is controlled at ≤25℃ throughout the process. Masterbatch post-processing: slow pressure reduction and depressurization, the liquid is filtered at ≤25℃, the crude extract is purified by membrane separation and concentrated at 38℃ vacuum low temperature to obtain paste-like bird's nest peptide masterbatch; Testing indicators: Bird's nest peptide molecular weight ≤1000Da, content ≥90%, activity retention rate 98.9%, no impurities.

[0041] Example 6 Example 6 provides a method for preparing a calming and sleep-aiding fragrance (for cosmetic applications) using biomass masterbatch. Core masterbatches: whole tea theanine masterbatch obtained in Example 2 (separately extracted, content ≥90%), agarwood essential oil masterbatch (prepared via route B, yield ≥1.5%), and corn germ SOD three-enzyme masterbatch obtained in Example 3; Compound formulation ratio: theanine masterbatch 1.5%, agarwood essential oil masterbatch 0.8%, sandalwood essential oil masterbatch 0.6%, SOD three-enzyme masterbatch 2.0%, cosmetic grade sustained-release matrix 95.1% (petrolatum:jojoba oil:beeswax = 5:3:2); Preparation process: The slow-release matrix is ​​heated to 45℃ to melt, the above masterbatch is added, high-speed homogenization (3000r / min) is carried out for 15min, vacuum degassing is carried out for 20min, and it is cooled to a paste at room temperature and aseptically filled to obtain the calming and sleep-aiding fragrance. Product effects: Theanine and agarwood / sandalwood essential oil work synergistically to calm the nerves, SOD three enzymes provide antioxidant protection, the paste matrix slowly releases active ingredients, the fragrance lasts for ≥12 hours, and it has a significant sleep-aiding effect.

[0042] Example 7 Example 7 provides a method for preparing antioxidant health food (solid beverage) using biomass masterbatch. Core masterbatches: Example 1: Willow tea melanin masterbatch; Example 2: Whole tea polyphenol masterbatch; Example 3: Corn germ SOD three-enzyme masterbatch; Example 4: Ginsenoside masterbatch; Compound formulation ratio: Willow tea melanin masterbatch: 5%, whole tea polyphenol masterbatch: 8%, corn germ SOD triple enzyme masterbatch: 10%. Five-year-old ginsenoside masterbatch: 3%, sweetener / prebiotic excipients: erythritol: 30%, fructooligosaccharides: 16%, inulin: 11%. Resistant dextrin: 6%, maltodextrin: 4%, Anti-inflammatory / antioxidant / lipid-lowering ingredients: Blueberry anthocyanins: 3%, Curcumin: 0.3%. Apigenin: 0.2%, Sulforaphane: 0.2%, Allicin: 0.08%, Chrysanthemum greens extract: 0.7%, Creatine: 1.5%, Ergothioneine: 0.08%, Polydeoxynucleotides: 0.22%; Preparation process: Mix all raw materials evenly, pulverize to 80 mesh, dry granulate, and fill into solid beverage; Product benefits: Four masterbatches work synergistically to fight oxidation and enhance immunity. No added sucrose, suitable for all types of people, and easy to mix.

[0043] Example 8 This embodiment 8 provides an air separation nitrogen production coupled with electrolytic hydrogen-rich water biomass extraction device, such as... Figure 1 As shown, this device consists of 12 core units. Each unit is physically connected to an intelligent electric quick-connect ball valve via DN25-DN80 food-grade 304 / 316L stainless steel quick-connect sealing pipes. The entire system is equipped with high-precision sensors to achieve automated, sealed, and clean operation. The core components are a pre-air nitrogen generation system 1, a nitrogen vapor explosion coupled core extraction host 5, a high-pressure nitrogen low-temperature extraction device 6, and a dedicated hydrogen-rich water dual-inlet and three-outlet pipeline structure. The front-end fully automated raw material pretreatment unit 3 is equipped with a dedicated electrolytic hydrogen-rich water soaking module for calming and sleep-aiding raw materials such as tea, agarwood / sandalwood, spikenard, and vetiver. The three-way parallel intelligent water supply system 2 features a dual-source water supply structure with branch lines for electrolytic hydrogen-rich water preparation. After electrolysis, a cathode hydrogen-rich water storage tank 212, an anode oxygen-rich water storage tank 213, and a dedicated hydrogen storage tank 214 are provided. The pre-air nitrogen generation system 1 achieves full recovery of nitrogen, oxygen, and water vapor. The closed-loop wastewater purification and reuse unit 8 achieves zero wastewater discharge. The entire process is operated unmanned by a fully sensor-linked manual / automatic dual-mode control system 13. The specific structure is as follows: like Figure 1As shown, the pre-air nitrogen generation system 1 provides high-purity nitrogen to the biomass extraction device. It consists of six main stages: a three-stage physical isolation filtration layer 101 (primary, medium, and high efficiency filters with filtration accuracies ≥5μm, ≥1μm, and ≥0.3μm respectively, equipped with a differential pressure sensor) → a deep purification and sterilization component (activated carbon adsorption tower, ultraviolet sterilizer, precision demister, equipped with temperature and humidity sensors) → an air compressor unit (screw type, compressed pressure 0.7-1.0MPa, temperature 25±5℃, equipped with pressure / temperature sensors) → a high-pressure low-temperature air separation unit (0.8-1.0MPa, -180℃, gas-liquid separation of crude nitrogen, by-product oxygen, and water vapor) → a nitrogen purification and pressurization component. (5A molecular sieve purification tower, nitrogen booster, purity increased to 99.999%, pressure 0.5-1.5MPa, equipped with buffer tank and pressure sensor) → By-product recovery component 102 (oxygen is dried and purified and then recovered to oxygen cylinders for sale; water vapor is condensed and returned to the tap water inlet; condensate is collected as clean water); nitrogen is output in multiple paths to the enzymatic hydrolysis tank 41 (inert protection), the core extraction host 5 (nitrogen spray cooling), the front-end pretreatment unit soaking tank (inert protection), and the high-pressure nitrogen low-temperature extraction device 6 (extraction gas source) to prevent oxidation of raw materials and active ingredients.

[0044] like Figure 5 As shown, the three-way parallel intelligent water supply system 2 includes branches for tap water, ion-exchange soft water, and RO reverse osmosis pure water, all equipped with electric valves, flow meters, and pressure sensors. The pure water branch is equipped with a conductivity sensor. An additional branch for preparing electrolyzed hydrogen-rich water is added, employing a dual-source supply structure. It can be connected to either ion-exchange water or RO reverse osmosis pure water and is linked to the pure water branch. It is equipped with an electrolysis module 21 using platinum, titanium, titanium alloy, or platinum-titanium composite electrodes, a pH sensor 423, and a hydrogen-rich concentration detector. It utilizes cathode electrolysis to prepare electrolyzed hydrogen-rich water with a pH of 9.0-10.0, and anode electrolysis to produce oxidizing water. A cathode hydrogen-rich water storage device is installed after electrolysis. Tank 212 is connected to anode oxygen-enriched water storage tank 213, and cathode hydrogen-enriched water storage tank 212 is connected to dedicated hydrogen storage tank 214. Hydrogen storage tank 214 supplies hydrogen to each hydrogen-using unit at regular intervals through a liquid level control system, which is used for hydrogen molecule permeation and swelling during raw material soaking. Anode oxygen-enriched water storage tank 213 has a closed structure, and the output oxygen-enriched water is used as front-end cleaning and sterilization water for CIP sterilization and cleaning, realizing full resource utilization of electrode water. Each branch can be automatically switched and precisely controlled to realize graded utilization of water resources, and a one-way valve is provided to prevent backflow contamination.

[0045] The hydrogen-rich water output end of the electrolytic hydrogen-rich water preparation branch is equipped with dual input pipes 22, both of which are sealed to the cathode outlet 211 of the electrolysis module 21. This dual-path supply of hydrogen-rich water, with one main and one backup, prevents supply interruption due to blockage of a single pipe. Each input pipe is equipped with a flow sensor 223, a check valve 224, and an electric shut-off valve 225, and is linked to a fully sensor-driven manual / automatic dual-mode control system 13. When the pipe flow rate is below 0.1 m³ / h or the pressure fluctuation exceeds ±0.05 MPa, a single pipe failure automatically switches to the backup input pipe 222, simultaneously triggering an audible and visual alarm. After the dual input pipes merge, they connect to a three-outlet intelligent control valve group 23. This valve group is an integrated structure made of food-grade 316L stainless steel, with a built-in high-precision electromagnetic reversing valve 231 and pressure... The regulator 232 and flow metering module 233 have three independent outlets, which are respectively sealed to the process water inlets of the nitrogen vapor explosion coupling core extraction host 5, enzymatic hydrolysis tank 41, and fermentation tank 42 through food-grade quick-install sealing pipes. Each outlet is equipped with an independent electric regulating valve 237, pressure sensor 238, and flow recorder, which can realize single / multi-channel simultaneous feeding. The feeding pressure is adjustable from 0.2 to 0.5 MPa, and the flow rate is precisely controlled from 0 to 5 m³ / h. The three-outlet intelligent control valve group 23 is electrically connected to the fully sensor-linked manual and automatic dual-mode control system 13, which can switch the feeding tank with one click according to the production process requirements, realize the automated and precise delivery of hydrogen-rich water to each tank, and record the feeding parameters in real time and support cGMP data traceability.

[0046] The front-end fully automated raw material pretreatment unit 3 realizes raw material cleaning, crushing, soaking, and pre-cooking. It adds dedicated processing sub-modules for calming and sleep-aiding raw materials such as tea, agarwood, sandalwood, spikenard, and vetiver. These sub-modules include dedicated soaking tanks for electrolytic hydrogen-rich water, automatic tea stem separation devices, and graded crushing components for calming and sleep-aiding raw materials. The dedicated soaking tanks are made of food-grade 316L stainless steel and are equipped with a temperature-controlled jacket (for ambient temperature soaking at 20-30℃), a nitrogen inlet, a stirring paddle (for low-speed breakage prevention), and level / temperature / pH sensors to achieve constant temperature and pH soaking for tea and calming and sleep-aiding raw materials. The hydrogen molecules' small molecule penetration ability is used to achieve swelling and decomposition of the raw material cellulose. The pretreatment tanks are all equipped with temperature-controlled jackets consistent with the core unit, maintaining a precise constant temperature of 25-90℃. They are equipped with material conveying pumps, crushing units, and level / temperature sensors. The tanks have a 360° rotation design. The spray cleaning ball enables automated continuous operation; the graded crushing component for calming and sleep-aiding raw materials is suitable for low-loss crushing of hard raw materials such as agarwood and sandalwood, and gentle crushing of herbal raw materials such as spikenard and vetiver to prevent the volatilization of essential oil components.

[0047] Among them, such as Figure 4As shown, the low-temperature enzymatic hydrolysis and low-temperature fermentation pretreatment unit 4 includes an enzymatic hydrolysis tank 41 and a fermentation tank 42, both equipped with a temperature control jacket, with temperature control ranging from 25-40℃ and an accuracy of ±0.5℃. Both the enzymatic hydrolysis tank 41 and the fermentation tank 42 are equipped with dedicated hydrogen-rich water inlets, which are sealed to the corresponding outlets of the three-outlet intelligent control valve group 23 to achieve precise hydrogen-rich water supply. The enzymatic hydrolysis tank 41 is a pressure-bearing structure, equipped with a frequency conversion stirring assembly 414, an enzymatic hydrolysis batching module, and a nitrogen inlet 412, providing full nitrogen protection. It is suitable for the low-temperature enzymatic hydrolysis of cellulase / pectinase after soaking tea leaves, agarwood, sandalwood, spikenard, vetiver, and other calming and sleep-aiding herbal raw materials, improving the dissolution rate of tea polyphenols, theanine, active flavonoids, terpenes, and other components. The fermentation tank 42 is an atmospheric pressure structure, without stirring, static fermentation, and without nitrogen protection, equipped with a sterile sampling port and a hydrophobic membrane. The activated carbon filter cartridge has an exhaust device 422 and a microbial inoculation module. The tank is equipped with pH / dissolved oxygen / temperature sensors 423. Both tanks are equipped with 360° spray cleaning balls and are covered with a 50mm polyurethane insulation layer.

[0048] Among them, such as Figure 3 As shown, the nitrogen-steam explosion coupling core extraction host 5 (hydrogen explosion tank) is the core of the biomass extraction device. It features a food-grade stainless steel pressure-resistant tank 501 with a wall thickness ≥8mm and an inner wall electrolytic polishing Ra≤0.6μm, reinforced with ribs. The tank is equipped with a dedicated hydrogen-rich water inlet, which is sealed to the main outlet of a three-outlet intelligent control valve group, enabling synergistic cell-breaking extraction of hydrogen-rich water with nitrogen and steam. Core temperature control: a single-layer heat-conducting oil temperature control jacket 502, with cold oil entering from the top and hot oil returning from the bottom by gravity, without a hot oil return pump, covered with a 50mm polyurethane insulation layer, and equipped with dual independent sealed oil tanks 503 for hot / cold oil (equipped with level / temperature sensors), an electric three-way switching valve, a dedicated variable frequency speed-regulating oil pump for cold oil (equipped with a pressure sensor), and a fully submerged electric heating element (with overheat / Dry-burn protection), temperature control 25-180℃, accuracy ±0.5℃; core functions: switchable between pure nitrogen high-pressure cell disruption, steam explosion, and nitrogen full-process protection modes. Suitable for tea raw materials, agarwood, sandalwood, spikenard, vetiver, and other calming and sleep-aiding raw materials, including the low-temperature steam explosion and nitrogen full-process protection modes, to prevent high-temperature damage to heat-sensitive components. Equipped with a steam supply system (pressure regulator, regulating valve, flow meter, sensor), nitrogen full-process protection, and dual-branch spray cooling; triple automatic cooling: after steam explosion, the pressure / temperature sensor triggers triple cooling linkage. Component 504 sequentially stops the electric heating element, switches the cold oil circulation, and starts the nitrogen spray, reducing the tank temperature to below 40℃ within 1-3 minutes; Auxiliary components: shearing and crushing component (with speed sensor, ultra-low speed shearing for extracting calming and sleep-aiding raw materials to prevent essential oil volatilization), sanitary scraping component, negative pressure defoaming system, ultrasonic-assisted cell wall breaking system, equipped with multi-dimensional sensor group and graded cell wall breaking control module; Safety and cleaning: equipped with automatic pressure relief valve, rupture disc, safety interlock device, 360° spray cleaning ball on the top, and all interfaces are food-grade quick-install structure.

[0049] The high-pressure nitrogen low-temperature extraction device 6 is connected to the discharge end of the nitrogen vapor explosion coupling core extraction host 5. It adopts a low-temperature high-pressure nitrogen extraction process with an extraction pressure of 0.5-1.2MPa and an extraction temperature of 20-40℃, which is suitable for the efficient extraction of heat-sensitive plant essential oils, terpenes and other components. It is electrically connected to the fully sensor-linked manual and automatic dual-mode control system 13 to achieve precise control of extraction parameters. After extraction, the liquid is transported to the dual-mode solid-liquid separation unit 7. The dual-mode solid-liquid separation unit 7 is a plate and frame filter press and a centrifugal dehydrator connected in parallel. Both are made of food-grade stainless steel and equipped with a material conveying pump and a liquid level / pressure sensor. The extract liquid of tea and the extract liquid of calming and sleep aid raw materials are both suitable for low-speed centrifugal dehydration mode (speed 3000-4000r / min) to prevent oxidation of tea polyphenols and plant essential oils and emulsification of the liquid. It can be automatically / manually switched according to the characteristics of the materials. After separation, the solid phase is sent to the residue recovery unit and the liquid phase is sent to the back-end purification unit, which is equipped with an online cleaning system.

[0050] The closed-loop wastewater purification and reuse unit 8 collects all wastewater from the biomass extraction device, including wastewater from the electrolysis of hydrogen-rich water after soaking tea leaves and calming / sleep-aiding ingredients. After pH neutralization and adjustment, the wastewater is fed into the treatment process, undergoing "grid filtration → equalization tank → anaerobic reaction tank → aerobic reaction tank → sedimentation tank → sand filtration → carbon filtration → nanofiltration → activated carbon adsorption → ultraviolet sterilization". The treated wastewater is then reused for equipment pre-washing and raw material cleaning, achieving zero discharge. The sludge is filtered and used as raw material for organic fertilizer. The entire unit is equipped with level / temperature / water quality sensors and operates automatically.

[0051] Unit 9, which is responsible for the collection, drying, and crushing of solid residues, allows the residues extracted from tea leaves to be made into tea powder, tea fertilizer, or feed ingredients. The residues extracted from agarwood, sandalwood, and other calming and sleep-aiding raw materials can be made into aromatherapy materials, health packs, and other by-products, thereby improving the comprehensive utilization rate of raw materials. It is equipped with a food-grade stainless steel dryer, crusher, and storage tank, and has a cleaning interface.

[0052] The 10-unit automated purification, freeze-drying, and packaging unit includes a precision filter, a chromatography column, a vacuum concentration tank, a freeze dryer, and an automated packaging machine. All tanks are equipped with temperature-controlled jackets for precise low-temperature temperature control, suitable for the low-temperature purification and concentration of heat-sensitive components such as tea polyphenols, theanine, and plant essential oils and terpenes used in calming and sleep-aiding products, preventing oxidation and deactivation of components. The entire unit is sealed and equipped with flow / level / temperature / purity sensors to achieve full automation of purification-drying-packaging, and includes a CIP cleaning interface. For calming and sleep-aiding essential oil components, a low-temperature vacuum distillation module is added to improve the purity and aroma quality of the essential oils, meeting the requirements of high-end daily chemical raw materials.

[0053] The CIP 360° fully automatic cleaning unit 11 connects to the tanks, equipment, and pipelines of the entire production line. It includes a cleaning water tank, an acid and alkali cleaning solution tank, a heating tank, a cleaning pump, and a spray ball. Equipped with concentration / temperature / flow sensors, it achieves full automation of clean water-acid-alkali-rinsing-neutralization-drying. It can perform thorough cleaning of tea soaking tanks, calming and sleep-aiding raw material soaking tanks, and shearing components. The oxidizing water from anodic electrolysis can be directly connected to this unit as sterilization and cleaning water. The cleaning waste liquid is connected to the closed-loop wastewater purification and reuse unit 8.

[0054] The cGMP workshop environmental control unit 12 achieves constant temperature (25±5℃), constant humidity (40-60%RH), and a cleanliness level of D or above in the workshop. It includes a central air conditioning system, negative pressure ventilation, waste gas treatment (activated carbon adsorption and photocatalysis), dehumidification, and ultraviolet sterilization system. It is equipped with temperature, humidity, cleanliness, and differential pressure sensors, and the parameters are uploaded in real time for automatic control, effectively preventing the oxidation of active ingredients and workshop pollution during the extraction of tea and calming and sleep-aiding raw materials. In view of the volatile nature of the essential oil components in calming and sleep-aiding products, a low-temperature essential oil recovery module for workshop waste gas is added to realize the resource utilization of by-products.

[0055] like Figure 6 As shown, the fully sensor-linked manual / automatic dual-mode control system 13 uses a PLC, touch screen, and industrial computer as its core, and is linked with all production line equipment, sensors, valves, and oil pumps. It includes a dedicated control module for hydrogen-rich water pipelines, which monitors the operating status, supply pressure, and flow parameters of the dual-input hydrogen-rich water pipelines and the three-outlet intelligent control valve group in real time, enabling automatic switching between the two pipelines, precise reversal of the three outlets, and automatic fault alarms. It also includes a triple cooling linkage control module to precisely control the cooling process of the core extraction host. Furthermore, it includes dedicated process formula modules for tea-based raw materials, agarwood, sandalwood, spikenard, vetiver, and other calming and sleep-aiding raw materials, with built-in tea and calming / sleep-aiding ingredients. The system provides optimal parameters for soaking, enzymatic hydrolysis, cell wall disruption, extraction, and extraction of various raw materials. A single click enables fully automated extraction of two types of raw materials. Automatic mode: After identifying raw material properties, process parameters are automatically matched. One-click start enables fully automated operation. Steam explosion automatically triggers triple cooling. All parameters are recorded, stored, and printed in real time, supporting cGMP traceability. Manual mode: Individual equipment can be controlled, adaptable for debugging and small-batch production. Additional functions: Comprehensive safety interlock protection (automatic shutdown alarms for over-temperature / over-pressure / material shortage / overload), remote monitoring (mobile / computer), formula storage and retrieval, data export, and an on-site emergency stop button.

[0056] This invention is applicable to all biomass raw materials, including plant-based materials (corn, sea buckthorn, ginseng, noni fruit, Chinese medicinal herbs, fruits and vegetables, agricultural by-products, tea (green tea, Pu'er tea, white tea and other types of tea), agarwood, sandalwood, spikenard, vetiver and other calming and sleep-aiding plants, etc.), animal-based materials (propolis, royal jelly, marine organisms, etc.), and microbial-based materials (yeast, probiotics, etc.). This invention applies to the following products: food additives, health food ingredients, traditional Chinese medicine extracts, daily chemical washing / skin care ingredients, feed additives, organic fertilizer ingredients, core ingredients for functional tea beverages (tea polyphenol powder, theanine powder, compound tea extracts, etc.), calming and sleep-aiding cosmetics, perfumes, and core ingredients for single-use ampoules (plant essential oils, compound calming extracts, etc.). The following are several examples of using this invention to produce biomass feedstocks: Sea buckthorn SOD and multi-enzyme extraction: Sea buckthorn is a heat-sensitive raw material, and a pure nitrogen high-pressure cell wall disruption process is used. 1. After washing and crushing, sea buckthorn is sent to an enzymatic hydrolysis tank. Hydrogen-rich water is supplied to the enzymatic hydrolysis tank through a three-outlet intelligent control valve group. Under constant temperature of 35℃ and nitrogen protection, pectinase / cellulase is added for 2 hours of enzymatic hydrolysis. 2. The material is fed into the hydrogen explosion tank. Hydrogen-rich water is supplied to the hydrogen explosion tank through a three-outlet intelligent control valve group. The temperature is kept constant at 35℃ and the nitrogen pressure is maintained at 0.8MPa for 30 minutes. The cell wall is broken by ultrasonic and shearing. 3. Centrifugation and dehydration separation, followed by purification, freeze drying, and automatic packaging into sea buckthorn SOD extract, and solid-phase feed preparation; 4. Fully automated operation, wastewater reuse, equipment CIP cleaning, dual-input pipeline for hydrogen-rich water supply along the main line, and real-time parameter recording.

[0057] Results: SOD activity retention rate ≥98%, extraction efficiency increased by more than 35%.

[0058] Corn husk dietary fiber extraction: Corn husk is a non-heat-sensitive raw material, and the extraction process employs steam explosion + nitrogen protection + triple cooling. 1. After washing and soaking the corn husks, they are sent to the hydrogen explosion tank. Hydrogen-rich water is supplied to the hydrogen explosion tank through a three-outlet intelligent control valve group. The tank is preheated at 80℃, protected by nitrogen throughout the process, and subjected to 1.2MPa, 180℃ steam explosion for 3 minutes. 2. The sensor triggers a triple cooling process, reducing the tank temperature to 38°C within 2 minutes; 3. After the material is crushed, it is filtered by plate and frame filter press. The liquid phase is purified and concentrated to produce dietary fiber extract, and the solid phase is utilized as a resource. 4. Fully automated operation, wastewater and condensate are recycled, hydrogen-rich water supply flow rate is 0.8 m³ / h, pressure is 0.3 MPa.

[0059] Results: Dietary fiber extraction rate ≥90%, purity ≥85%, and added value of corn husk increased by more than 8 times.

[0060] Ginseng polysaccharide / ginsenoside extraction: Ginseng is a heat-sensitive raw material that is difficult to break down. The extraction process employs low-temperature enzymatic hydrolysis + static fermentation + high-pressure cell disruption using pure nitrogen. 1. After ginseng slices are placed into an enzymatic hydrolysis tank, hydrogen-rich water is supplied to the tank through a three-outlet intelligent control valve group. Enzymatic hydrolysis is carried out for 3 hours under constant temperature of 38℃ and nitrogen protection with cellulase / pectinase. 2. The material is fed into the fermenter, and hydrogen-rich water is supplied to the fermenter through a three-outlet intelligent control valve group. Static fermentation is carried out at 35℃ for 48 hours without nitrogen protection. 3. After fermentation, the material is sent to the hydrogen explosion tank. Hydrogen-rich water is supplied to the hydrogen explosion tank through a three-outlet intelligent control valve group. The temperature is kept constant at 40℃ and the nitrogen pressure is maintained at 0.9MPa for 40 minutes. The cell wall is broken by ultrasonic and shearing. 4. After centrifugation and dehydration, the extract is purified and freeze-dried to produce the final product, thus realizing solid-phase resource utilization.

[0061] Results: The extraction rate of ginseng polysaccharides was ≥85%, the extraction rate of ginsenosides was ≥80%, and the retention rate of heat-sensitive components was ≥96%.

[0062] Noni fruit enzyme extract: Noni fruit is extracted using a low-temperature static fermentation + low-pressure nitrogen cell wall disruption process. 1. After the noni fruit is cleaned and crushed, it is sent to the fermentation tank. Hydrogen-rich water is supplied to the fermentation tank through a three-outlet intelligent control valve group. Static fermentation is carried out at 36℃ for 60 days without nitrogen protection. Samples are taken for testing periodically. 2. After fermentation, the material is sent to the hydrogen explosion tank. Hydrogen-rich water is supplied to the hydrogen explosion tank through a three-outlet intelligent control valve group. The cell wall is broken by maintaining a constant temperature of 35°C and a nitrogen pressure of 0.3MPa for 20 minutes. 3. After centrifugation and dehydration, noni fruit enzyme stock solution is prepared, purified, sterilized, and automatically filled, thus realizing solid-phase resource utilization.

[0063] Results: The retention rate of active ingredients was ≥97%, the shelf life of the original solution was ≥12 months, and the taste was stable.

[0064] Tea (green tea) polyphenol and theanine extraction: Tea is a heat-sensitive and hard raw material. The extraction process employs a platinum electrode electrolytic hydrogen-rich water soaking pretreatment + low-temperature steam explosion + nitrogen protection throughout the process + triple cooling. 1. After the green tea stems are automatically separated, it is rinsed with running water for 3 minutes and then sent to a dedicated steeping tank. It is steeped at a constant temperature of 25℃ for 15 minutes in pH 9.5 electrolyzed hydrogen-rich water prepared using a platinum electrode cathode. The tea leaves are stirred at low speed to prevent damage and protected by nitrogen microbubble aeration. 2. After soaking, the tea leaves are cut into 1cm segments and sent to the enzymatic hydrolysis tank. Hydrogen-rich water (flow rate 0.5m³ / h, pressure 0.2MPa) is supplied to the enzymatic hydrolysis tank through a three-outlet intelligent control valve group. Under constant temperature of 38℃ and nitrogen protection, 0.8% cellulase is added for 3 hours for enzymatic hydrolysis. 3. The material is fed into the hydrogen explosion tank. Hydrogen-rich water (flow rate 1.0 m³ / h, pressure 0.4 MPa) is supplied to the hydrogen explosion tank through a three-outlet intelligent control valve group. Nitrogen protection is provided throughout the process. After preheating at 80℃, the material is exploded for 20 seconds with medium and low temperature steam at 0.9 MPa and 130℃. 4. The sensor triggers a triple cooling process, reducing the tank temperature to 30°C within 2 minutes. Subsequently, the nitrogen pressure is maintained at 1.0 MPa for 1.5 hours, followed by ultrasonic-assisted cell disruption. 5. The liquid phase is dehydrated by low-speed centrifugation at 3500 r / min. After precision filtration, chromatography purification, and vacuum concentration at 38℃, it is freeze-dried to produce tea polyphenol + theanine composite dry powder. The solid tea residue is made into tea powder. 6. The entire process is automated. The soaking wastewater is neutralized by pH and then reused. The oxidizing water prepared by the platinum electrode anode is directly used for sterilization and cleaning of the CIP unit equipment, with no waste of water resources. The hydrogen-rich water pipeline operates without failure throughout the entire process.

[0065] Results: The extraction rate of tea polyphenols was ≥90% and the content was ≥47%, the extraction rate of theanine was ≥85% and the content was ≥13%, the activity retention rate of both was ≥98%, and there were no pesticide residues or heavy metal residues.

[0066] Extraction of a compound calming extract from agarwood, sandalwood, spikenard, and vetiver: Agarwood, sandalwood, spikenard, and vetiver are thermosensitive, high-end calming and sleep-aiding raw materials. The core components are thermosensitive essential oils and terpenes. The process employs platinum electrode electrolytic hydrogen-rich water soaking pretreatment + low-temperature steam explosion + nitrogen protection throughout the process + triple cooling + low-temperature distillation. 1. Agarwood / sandalwood is graded and crushed into 2-3mm particles. Nardostachys jatamansi / vetiver are gently crushed and mixed together. The mixture is then sent to a dedicated soaking tank and soaked in pH 9.2 electrolyzed hydrogen-rich water prepared using a platinum electrode cathode at a constant temperature of 22℃ for 20 minutes. Ultra-low speed stirring is used to prevent damage to the raw materials, and nitrogen microbubble aeration is used throughout the process to prevent oxidation of essential oil components. 2. After soaking, the mixed raw materials are sent into the enzymatic hydrolysis tank. Hydrogen-rich water (flow rate 0.3 m³ / h, pressure 0.2 MPa) is supplied to the enzymatic hydrolysis tank through a three-outlet intelligent control valve group. Under constant temperature of 35℃ and nitrogen protection, 0.5% cellulase is added for enzymatic hydrolysis for 2.5 hours with ultra-low speed stirring (10 r / min). 3. The material is fed into the hydrogen explosion tank. Hydrogen-rich water (flow rate 0.8 m³ / h, pressure 0.3 MPa) is supplied to the hydrogen explosion tank through a three-outlet intelligent control valve group. Nitrogen gas is used for full-process sealing protection. After preheating at 75℃, the material is exploded with 0.8 MPa, 120℃ ultra-low temperature steam for 15 seconds to maximize the preservation of essential oil components. 4. The sensor triggers triple cooling, and the temperature of the tank drops to 28°C within 1.5 minutes. Then, the nitrogen pressure is maintained at 0.9MPa for 2 hours, and low-frequency ultrasonic waves are used to assist in cell wall breaking (to avoid high-frequency damage to the essential oils). 5. The liquid phase is dehydrated by ultra-low speed centrifugation at 3000r / min. After precision filtration and vacuum concentration at 35℃, it is connected to a low-temperature vacuum distillation module to purify the calming plant essential oil. The remaining extract after distillation is used to prepare a compound calming extract paste. 6. After the essential oil and extract are freeze-dried / spray-dried respectively, a calming essential oil powder and a compound calming extract powder are prepared. The solid residue is dried and pulverized to make an aromatherapy health pack. 7. The entire process is automated and closed. The soaking wastewater is reused after pH neutralization. The anodic oxidation water is used for CIP sterilization and cleaning. The workshop exhaust gas is collected by the essential oil recovery module to collect trace essential oils, realizing full-process resource utilization. The hydrogen-rich water supply parameters are recorded and traceable throughout the process.

[0067] Results: The extraction rate of calming plant essential oils was ≥88%, the retention rate of terpenoid components was ≥97%, the content of active flavonoids in the compound extract was ≥25%, there were no pesticide residues or heavy metal residues, and the added value of the raw materials was increased by more than 15 times, fully meeting the standards for high-end daily chemical raw materials for calming and sleep-aiding cosmetics, perfumes, and single-use essences.

[0068] Example 9 Preparation of anti-aging compound masterbatch for gut-brain axis-regulated microbiome (Pathway B: heat-sensitive raw materials + low-temperature fermentation) This embodiment relies on an air separation nitrogen generation coupled with electrolytic hydrogen-rich water biomass extraction device, and adopts a process of low-temperature hydrogen-rich water extraction + low-temperature enzymatic fermentation + nitrogen protection throughout the process to prepare a gut-brain axis-regulated microbiome anti-aging composite masterbatch containing probiotics + prebiotics + post-biotics + plant active factors. The raw materials are all heat-sensitive medicinal and edible materials, and the temperature is controlled at ≤35℃ throughout the process to protect the activity of probiotics and active ingredients such as plant polyphenols and organic acids. All process parameters and device units are consistent with the aforementioned technical solution of this invention.

[0069] Raw material pretreatment: Select plum powder (60 mesh), noni fruit powder (60 mesh), blueberry freeze-dried powder (80 mesh), and mulberry powder (80 mesh) and mix them in a weight ratio of 8:5:4:3 to obtain a compound plant matrix powder; Select AKK bacteria (Akkermansia muciniphila) powder, EM compound bacterial liquid, and Lactobacillus plantarum + Bifidobacterium compound probiotic powder and mix them in a weight ratio of 0.8:1.0:0.7 to obtain a compound functional bacterial agent; All raw materials are coarsely screened to remove impurities by the automatic pretreatment unit at the front end of the device, and the dry powder can be used directly without drying and is ready for use.

[0070] Low-temperature extraction with hydrogen-rich water: 1 kg of composite plant matrix powder is added to the nitrogen vapor explosion coupling core extraction host tank, and 1000 ppb of hydrogen-rich water produced by the device is injected. The liquid-to-material ratio is 10:1, the tank pressure is 0.3 MPa, the extraction temperature is 22℃, the low-speed shearing is 15 r / min, and the extraction time is 3.5 h. High-purity nitrogen gas with a slight positive pressure of 0.1 MPa is introduced throughout the process to isolate air. The hydrogen-rich water and nitrogen gas provide dual antioxidant protection to prevent the oxidation and inactivation of plant active ingredients.

[0071] Low-temperature enzymatic hydrolysis + symbiotic fermentation: 1. Enzymatic hydrolysis: The above hydrogen-rich water extract is transported to the enzymatic hydrolysis tank of the low-temperature enzymatic hydrolysis and low-temperature fermentation pretreatment unit. Food-grade pectinase and cellulase compound enzyme preparation is added, with a total addition amount of 0.6% of the dry weight of the plant substrate. High-purity nitrogen is introduced to maintain a slight positive pressure inside the tank. The temperature is controlled at 32℃, and the mixture is stirred at a low speed of 10r / min for 4 hours to break down the plant cell walls and fully release prebiotic components such as pectin polysaccharides and fructooligosaccharides. 2. Symbiotic Fermentation: After enzymatic hydrolysis, the liquid is cooled to 30°C and transported to the fermenter of the low-temperature enzymatic hydrolysis and low-temperature fermentation pretreatment unit. 800ppb of hydrogen-rich water is produced by the fermenter's dedicated hydrogen-rich water inlet replenishment device to adjust the liquid's moisture content to 65%. The aforementioned compound functional bacterial agent is then introduced. The entire process is static fermentation with nitrogen micro-positive pressure protection. The fermentation temperature is 30±0.5°C, and the fermentation time is 72 hours. The fermentation process is monitored in real time by a pH sensor, and the natural pH of the liquid is maintained at 3.5~4.5, which is suitable for the symbiotic growth environment of EM compound bacteria, AKK bacteria, Lactobacillus plantarum, and Bifidobacterium.

[0072] Nitrogen low-temperature high-pressure extraction: After fermentation, the fermentation liquid is transported back to the nitrogen steam explosion coupling core extraction host tank, and high-purity nitrogen produced by the device is introduced. The extraction pressure is controlled at 0.5MPa and the extraction temperature at 25℃. The nitrogen micro-positive pressure extraction is maintained for 4 hours, and the temperature is controlled at ≤25℃ throughout the process. This fully dissolves the short-chain fatty acids, small molecule peptides and other post-biotic components produced by fermentation, as well as the anthocyanins, polyphenols and other active factors in the plant matrix, while protecting the activity of probiotic cells.

[0073] Post-processing of masterbatch: After extraction, the pressure is slowly reduced and released. The liquid is separated by low-temperature centrifugation at ≤25℃ and a centrifugation speed of 3000r / min. Low-speed centrifugation prevents damage to the probiotic cells. The separated liquid phase is purified by microfiltration and ultrafiltration. Microfiltration removes impurities, while ultrafiltration retains probiotic cells and active small molecules. The purified liquid is then concentrated at 38℃ under vacuum to a solid content of 30% to obtain a paste-like gut-brain axis regulating microbiome anti-aging composite masterbatch. After sealing, it is stored at 4~10℃ in the dark.

[0074] Detection index: In the gut-brain axis-regulated microbiome anti-aging compound masterbatch prepared in this embodiment, the total viable count of probiotics is ≥1.0×10⁻⁶. 10 CFU / g, of which AKK viable count ≥ 5.0 × 10⁻⁶ 8 CFU / g; total content of prebiotic components (fructooligosaccharides + pectin polysaccharides) ≥15%, content of postbiotic components (short-chain fatty acids) ≥2.5%; total content of plant active ingredients (anthocyanins + polyphenols) ≥8%; retention rate of all active ingredients ≥98.5%, impurity content ≤4%, all indicators meet the quality requirements of the high-activity biomass masterbatch of this invention.

[0075] Application: The gut-brain axis-regulated microbiome anti-aging composite masterbatch prepared in this embodiment is an extension of the high-activity biomass masterbatch of this invention. It can be used alone or in combination with food-grade matrix to prepare functional beverages, oral liquids, solid beverages and other foods or health foods for intestinal conditioning and microbiome anti-aging. By regulating the balance of intestinal microecology, repairing intestinal barrier function and regulating gut-brain axis signal transduction, it can achieve the effects of intervening in the aging process from the source of the intestine, improving metabolism and enhancing the body's immunity.

[0076] Example 10: Intestinal Microecology Regulation and Anti-aging Functional Beverage This embodiment uses a gut-brain axis-regulated microbiome anti-aging compound masterbatch as the core raw material to prepare a functional beverage for gut microecology regulation and anti-aging. The specific formula and process are as follows: Core ingredients: 30% gut-brain axis-regulated microbiome anti-aging composite masterbatch (paste) prepared in this embodiment, 5% erythritol, 3% xylooligosaccharide, 0.2% food-grade sodium citrate, 0.1% edible flavoring, and 61.7% RO pure water produced by the device itself; Preparation process: Preheat RO pure water to 25℃, add erythritol, xylooligosaccharide, and sodium citrate in sequence, stir until completely dissolved, cool to ≤20℃, add gut-brain axis regulating microbiome anti-aging complex masterbatch and edible flavoring, homogenize at low speed (500r / min) for 10min, with nitrogen micro-positive pressure protection throughout to prevent probiotic inactivation; after homogenization, aseptically filter (0.22μm filter membrane), aseptically cold fill into food-grade PET bottles, seal and store at 4~10℃; Product Specifications: Total live probiotic count in beverages ≥ 3.0 × 10⁻⁶ 9 CFU / mL, pH 3.8~4.5, soluble solids ≥8%; no preservatives added, shelf life under refrigeration ≥6 months, and all microbiological indicators meet the relevant requirements of GB 7101 "Hygienic Standard for Beverages" and GB 19301 "National Standard for Food Safety of Raw Milk"; Product efficacy: Drinking 200mL daily can effectively regulate the abundance of beneficial bacteria in the gut, enhance the integrity of the intestinal barrier, reduce the level of chronic inflammation in the body, and at the same time improve sleep quality and enhance physical energy through the gut-brain axis, achieving anti-aging of the gut microecology and regulation of the whole body's functions.

[0077] All processes in this embodiment are completed using the air separation nitrogen generation coupled with electrolytic hydrogen-rich water biomass extraction device of the present invention. The parameters such as hydrogen-rich water concentration, nitrogen pressure, extraction / fermentation / post-treatment temperature are consistent with the extraction parameters of heat-sensitive raw materials in path B of the present invention. No new equipment or process steps are added. The prepared gut-brain axis-regulated microbiome anti-aging composite masterbatch and end products are all within the scope of the high-activity biomass masterbatch and its application of the present invention.

[0078] Example 11: Preparation of an anti-aging essence using hydrogen-rich water, biomass masterbatch, and nonapeptide-1 Raw material preparation: Example 8: 1000ppb hydrogen-rich water (solvent) produced by the device itself; Example 2: whole tea polyphenol / theanine composite masterbatch; nonapeptide-1; hexapeptide-8; sodium hyaluronate. Formula (mass%): Hydrogen-rich water: 88%, Tea polyphenol / theanine compound masterbatch: 5%, Polypeptide combination (nonapeptide-1 + hexapeptide-8 + copper peptide): 0.15%, Sodium hyaluronate: 2.5%, Polygonum cuspidatum root extract: 1.5%, Panthenol: 1.2%, Tranexamic acid: 1.65%; Preparation process: Add hydrogen-rich water to a sterile mixing tank, add composite masterbatch and sodium hyaluronate sequentially at a constant temperature of 30°C, stir at low speed (200r / min) until completely dissolved; then add nonapeptide-1 and hexapeptide-8, continue stirring for 15min, filter through a 0.22μm microporous membrane for sterilization, and aseptically fill to obtain anti-aging essence. Testing indicators: DPPH free radical scavenging rate ≥88%, active ingredient retention rate ≥99%, non-irritating to the skin, and a reduction of fine lines by ≥20% after 28 days of continuous use.

[0079] Example 12: Preparation of a whitening and spot-fading cream using hydrogen-rich water, biomass masterbatch, and nonapeptide-1 Raw material preparation: 800ppb hydrogen-rich water produced by the device in Example 8, tea melanin masterbatch from willow tea in Example 1, nonapeptide-1, 5% niacinamide, squalane, and cosmetic-grade cream base; Formula (mass%): 85% hydrogen-rich water, 4% willow tea melanin masterbatch, 0.05% nonapeptide-1, 5% nicotinamide, 1.95% squalane, 4% cream base; Preparation process: Hydrogen-rich water is mixed with cream base and squalane, and homogenized at 45℃ at high speed (3000r / min) for 10min to obtain cream base; after cooling to 30℃, willow tea melanin masterbatch, nonapeptide-1 and niacinamide are added in sequence, stirred until uniform, vacuum degassing for 20min, and aseptically filled to obtain whitening and spot-fading cream. Testing indicators: Tyrosinase inhibition rate ≥75%, no skin irritation, and after 45 days of continuous use, skin pigmentation area is reduced by ≥18% and skin tone brightness is improved by ≥15%.

[0080] Example 13: Preparation of Sensitive Skin Repair Ampoules from Hydrogen-Rich Water and Biomass Masterbatch Raw material preparation: Example 8: 1200ppb hydrogen-rich water produced by the device itself; Example 3: corn germ SOD three-enzyme masterbatch; ceramide NP; β-glucan; Formula (mass%): 94% hydrogen-rich water, 3% SOD triple enzyme masterbatch, 1.5% ceramide NP, 1.5% β-glucan; Preparation process: Add hydrogen-rich water to a sterile mixing tank, add SOD three-enzyme masterbatch at a constant temperature of 25℃, and stir at low speed until dissolved; then add ceramide NP and β-glucan, stir for 20 minutes until completely dispersed, filter through a 0.22μm microporous membrane for sterilization, fill into ampoule bottles, and seal to obtain sensitive skin repair ampoules; Testing indicators: SOD activity ≥500U / mL, can quickly soothe skin redness, non-irritating to sensitive skin, and can improve skin repair speed by ≥30% when used after medical aesthetic procedures.

[0081] Example 14: Preparation of Hydrogen-Rich Water Anti-Aging Oral Liquid (Portable Strip Packet) This embodiment uses the hydrogen-rich water produced by the device described in Example 8 as the core solvent, combined with the highly active biomass masterbatch prepared by this invention, to prepare oral beauty and anti-aging functional food. It is suitable for both oral liquid bottle and portable strip pack dosage forms. The process is simple and can be mass-produced. It forms a synergistic effect system of "internal regulation + external nourishment" with the external cosmetic of this invention.

[0082] Raw material preparation: The device described in Example 8 produces 1000ppb hydrogen-rich water (conductivity ≤10μS / cm, solvent + active carrier), whole tea polyphenol / theanine composite masterbatch, corn germ SOD three-enzyme masterbatch, five-year-old ginsenoside masterbatch, astragalus extract, polygonatum extract, jujube powder, wolfberry powder (core for replenishing qi and blood), and food-grade fructooligosaccharides (lightly sweetened flavoring + prebiotics, no additional preservatives / pigments / flavors); Product composition (total mass 100%, applicable to oral liquid / strip): Hydrogen-rich water: 75~82%, Tea polyphenol / theanine compound masterbatch: 2~4%, Corn germ SOD triple enzyme masterbatch: 3~5%, Five-year-old ginsenoside masterbatch: 1~3%, Food and medicine ingredients for replenishing qi and blood: Donkey-hide gelatin extract: 2~3%, Rose extract: 1~2%, Goji berry powder: 2~3%, Longan powder: 1~2%, Red date powder: 2~3%, Astragalus extract: 1~2%, Prebiotic flavoring: Fructooligosaccharides: 1~3%; Preparation process: (1) The hydrogen-rich water produced by the device is introduced into a sterile mixing tank and kept at a constant temperature of 30±2℃. The entire process is protected by nitrogen micro-positive pressure (to prevent hydrogen molecule loss and oxidation of active ingredients). (2) Add the composite masterbatch, SOD three-enzyme masterbatch and ginsenoside masterbatch in sequence, and stir at low speed (150-200r / min) for 15min until dissolved; (3) Add Astragalus extract, Polygonatum extract, jujube powder, and wolfberry powder, and continue stirring for 20 minutes until homogeneous and free of particles; (4) Add fructooligosaccharides for flavoring and stir for 10 minutes until transparent; (5) Aseptic filtration with 0.22μm microporous membrane, filled as needed (50mL glass bottle / 10mL aluminum foil strip pack). (6) Pasteurize at low temperature (65℃ / 30min, retaining all active ingredients), cool and label, and store in a light-proof and sealed container; Testing indicators and efficacy characteristics: (1) Activity indicators: DPPH free radical scavenging rate ≥90%, SOD activity ≥800U / mL, brightening and removing yellowing, improving dull skin, and mild anti-aging; (2) Stability indicators: After being stored at room temperature in a sealed container away from light for 6 months, the hydrogen concentration retention rate of hydrogen-rich water is ≥85%, the retention rate of each active ingredient is ≥95%, and there is no stratification, no precipitation, and no odor. (3) Safety and efficacy: No added preservatives, pigments and fragrances, and non-irritating to the human body; long-term use can achieve the beauty effects of anti-oxidation, brightening and yellowing, improving dull skin and mild anti-aging. When used in combination with the hydrogen-rich water external efficacy cosmetic of this invention, it can regulate internal and nourish externally and enhance the effect. (4) Astragalus + Polygonatum replenishes Qi and nourishes Yin, red dates + wolfberry nourish blood and calm the mind. Combined with ginsenosides, it can improve the sallow complexion, fatigue and insomnia caused by insufficient Qi and blood, and is suitable for women's daily internal conditioning. (5) No added preservatives / pigments / fragrances, can be stored at room temperature away from light for 6 months, retains ≥95% of active ingredients, has good mixing properties, and is ready to drink immediately after opening. (6) Dosage form compatibility: The oral liquid glass bottle is suitable for high-end gift box packaging, and the 10mL portable strip pack is suitable for daily commuting and can be opened and drunk immediately. It is suitable for sales in multiple scenarios such as supermarkets, beauty channels, and online e-commerce.

[0083] Example 15: Preparation of Hydrogen-Rich Water Male Energy and Anti-Fatigue Oral Liquid (Portable Strip Packet) This embodiment uses the hydrogen-rich water produced by the device described in Example 8 as the core solvent, and combines it with the highly active biomass masterbatch prepared by this invention and male tonic food-grade medicinal materials to prepare an oral anti-fatigue and energy-boosting functional food. It is suitable for both oral liquid bottle and portable strip packaging, forming a dual-track internal conditioning product matrix for men and women with the female beauty and anti-aging oral liquid of this invention.

[0084] Raw material preparation: The device described in Example 8 produces 1000ppb hydrogen-rich water (conductivity ≤10μS / cm, solvent + active carrier), whole tea polyphenol / theanine composite masterbatch, corn germ SOD three-enzyme masterbatch, five-year-old ginsenoside masterbatch, oyster peptide powder, zinc citrate, Cistanche deserticola extract, Citrus medica extract, Phyllanthus emblica powder, Cornus officinalis extract, Eucommia ulmoides extract (kidney-tonifying and energy-boosting core), and food-grade fructooligosaccharides (lightly sweet flavoring + prebiotics); Weight ratio (total weight 100%, applicable to both oral liquid and tablet packets): Tea polyphenol / theanine compound masterbatch: 2~4%, Corn germ SOD triple enzyme masterbatch: 3-5%, five-year-old ginsenoside masterbatch: 1-3%, medicinal and edible kidney-tonifying and essence-boosting raw materials: oyster peptide powder: 2-4%, Cistanche deserticola extract: 2-3%, Eucommia ulmoides extract: 1-2%, Citrus medica extract: 1-2%, Astragalus membranaceus extract: 1-2%, minerals: zinc citrate: 0.5-1%, prebiotic flavoring: fructooligosaccharides: 1-3%; Preparation process: (1) The hydrogen-rich water produced by the device is introduced into a sterile mixing tank and kept at a constant temperature of 30±2℃ with nitrogen micro-positive pressure protection throughout the process. (2) Add the compound masterbatch, SOD three-enzyme masterbatch, ginsenoside masterbatch, oyster peptide powder and zinc citrate in sequence, and stir at low speed (150-200r / min) for 20min until dissolved; (3) Add Cistanche deserticola, Citrus medica, Cornus officinalis, Eucommia ulmoides extract and Phyllanthus emblica powder, and continue stirring for 25 minutes until homogeneous and free of particles; (4) Add fructooligosaccharides for flavoring and stir for 10 minutes until transparent; (5) Aseptic filtration with 0.22μm microporous membrane, filled as needed (50mL glass bottle / 10mL aluminum foil strip pack). (6) Pasteurize at low temperature (65℃ / 30min), cool and label, and store in a sealed container away from light; Testing indicators and efficacy characteristics: (1) Activity indicators: DPPH free radical scavenging rate ≥92%, SOD activity ≥1000U / mL, oyster peptide + zinc replenish energy and improve post-exercise fatigue and daily endurance deficiency; (2) Stability indicators: After being stored at room temperature in a sealed container away from light for 6 months, the hydrogen concentration retention rate of hydrogen-rich water is ≥85%, the retention rate of each active ingredient is ≥95%, and there is no stratification, no precipitation, and no odor. (3) Safety and efficacy: No added preservatives, pigments, or fragrances, and no hormones / Western medicine ingredients. It is non-irritating to the human body. Long-term use can achieve the effects of anti-oxidation, anti-fatigue, boosting energy, improving endurance, and maintaining male reproductive health. When used in combination with the hydrogen-rich water external functional cosmetic of this invention, it can regulate internally and nourish externally, and synergistically enhance the effect. (4) Cistanche deserticola + Eucommia ulmoides tonifies kidney yang and benefits essence and blood, Cornus officinalis strengthens kidney and benefits essence, Citrus medica regulates qi and harmonizes the stomach (prevents greasy stomach from tonifying), Phyllanthus emblica clears heat and generates fluids, combined with ginsenosides, enhances energy and improves soreness and weakness of the waist and knees, suitable for men's daily tonification and recovery after exercise; (5) No hormones / Western medicine ingredients, no preservatives / pigments / fragrances, can be stored at room temperature away from light for 6 months, and the retention rate of probiotics / active ingredients is ≥95%. It can be consumed before and after exercise and during daily commutes. (6) Dosage form compatibility: The oral liquid glass bottle is suitable for high-end gift box packaging, and the 10mL portable strip pack is suitable for daily commuting and drinking before and after exercise. It is suitable for sales in multiple scenarios such as supermarkets, sports nutrition channels, and online e-commerce.

[0085] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for preparing highly active biomass masterbatch by coupling hydrogen-rich water pretreatment with air separation nitrogen production steam explosion, characterized in that, This includes general pretreatment of raw materials and any of the following extraction pathways: Path A: Low-temperature soaking pretreatment with hydrogen-rich water → High-temperature steam explosion cell disruption → Rapid cooling with nitrogen → Low-temperature high-pressure extraction with hydrogen-rich water and nitrogen → Post-treatment of masterbatch, used for the extraction of non-heat-sensitive / high-lignification raw materials; Route B: Low-temperature extraction with hydrogen-rich water → Low-temperature high-pressure extraction with nitrogen → Low-temperature post-treatment of the mother material, used for the extraction of heat-sensitive raw materials; The extraction path is achieved using an air separation nitrogen generation coupled with electrolysis of hydrogen-rich water biomass extraction device. The device produces high-pressure nitrogen with a purity ≥99.999%, hydrogen-rich water with a purity of 800-1200 ppb, and RO pure water with a conductivity ≤10 μS / cm. The device includes a pre-air nitrogen generation system, a three-way parallel intelligent water supply system, a front-end fully automatic raw material pretreatment unit, a low-temperature enzymatic hydrolysis and low-temperature fermentation pretreatment unit, a nitrogen vapor explosion coupled core extraction host, a dual-mode solid-liquid separation unit, a closed-loop wastewater purification and reuse unit, a residue recovery and resource utilization unit, a back-end purification freeze-drying automatic packaging unit, a CIP 360° fully automatic cleaning unit, a cGMP workshop environmental control unit, and a fully sensor-linked manual and automatic dual-mode control system. Each unit is seamlessly connected through food-grade 304 / 316L stainless steel quick-connect sealing pipes. The three-way parallel intelligent water supply system has an electrolytic hydrogen-rich water preparation branch, which is equipped with a dedicated hydrogen-rich water dual-inlet and three-outlet pipeline structure. The entire system is equipped with high-precision sensors.

2. The method according to claim 1, characterized in that, The general pretreatment of the raw materials is as follows: after coarse washing with water, the raw materials are crushed to 40-80 mesh; non-heat-sensitive / high-lignified raw materials are dried at a low temperature of 40-50℃ to a moisture content of 10-15%; and heat-sensitive raw materials are used directly as fresh materials.

3. The method according to claim 1, characterized in that, The specific parameters of path A are as follows: The hydrogen-rich water soaking solution has a ratio of 5:1 to 8:1, a pressure of 0.3 to 0.5 MPa, a temperature of 4 to 10°C, and a time of 24 to 36 hours. Steam explosion pressure: 1.0~1.2MPa; temperature: 180~200℃; pressure holding time: 10~30s. Nitrogen gas is used for cooling. The inlet pressure is 0.5~0.8MPa, and the temperature drops to below 40℃ in 1-3 minutes. The extraction liquid-to-material ratio is 10:1 to 15:1, the temperature is 35 to 45℃, the shearing speed is 20 to 40 r / min, and the time is 2 to 3 h. When using enzymatic hydrolysis-assisted extraction, the amount of biological enzyme added is 0.5 to 1.0% of the dry weight of the raw material, the enzymatic hydrolysis temperature is 30 to 40℃, and the time is 4 to 6 h.

4. The method according to claim 1, characterized in that, The specific parameters of path B are as follows: hydrogen-rich water extraction liquid-to-solid ratio 8:1~12:1, pressure 0.2~0.4MPa, temperature ≤25℃, shearing speed 10~20r / min, time 3~4h, food-grade extraction aids can be added; nitrogen extraction pressure 0.4~0.8MPa, temperature 20~25℃, time 3~5h, temperature controlled ≤25℃ throughout the process, and pressure is slowly reduced and released.

5. The method according to any one of claims 1-4, characterized in that, The highly active biomass masterbatch includes, but is not limited to: willow tea melanin masterbatch, whole tea polyphenol / theanine / catechin compound masterbatch, corn germ SOD three-enzyme masterbatch, five-year-old ginsenoside masterbatch, and bird's nest peptide masterbatch. The retention rate of active ingredients in the masterbatch is ≥98%, and the impurity content is ≤5%.

6. The method according to claim 5, characterized in that, The corn germ SOD three-enzyme masterbatch was prepared by adding cellulase and papain compound enzymatic hydrolysis, with SOD activity ≥10000U / g and CAT and POD enzyme activities ≥500U / g; the five-year-old ginsenoside masterbatch has a total saponin content ≥85% and Rg1+Re+Rb1 content ≥30%.

7. A highly active biomass masterbatch, characterized in that, The product is prepared by any one of the methods described in claims 1-6, including but not limited to one or more of the following: tea melanin masterbatch from willow tea, tea polyphenol / theanine / catechin composite masterbatch from whole tea, SOD three-enzyme masterbatch from corn germ, ginsenoside masterbatch from five-year-old ginseng, and bird's nest peptide masterbatch.

8. An application of the highly active biomass masterbatch as described in claim 7, characterized in that, It can be applied to the preparation of food, health food, and cosmetics, among other things.

9. An application of the highly active biomass masterbatch as described in claim 8, characterized in that, The cosmetic product is a calming and sleep-aiding fragrance, prepared from the following weight ratios: 1.0-2.0% theanine masterbatch, 0.5-1.0% agarwood essential oil masterbatch, 0.5-0.8% sandalwood essential oil masterbatch, 1.5-2.5% SOD triple enzyme masterbatch, and 94-96% cosmetic-grade slow-release matrix.

10. An application of the highly active biomass masterbatch as described in claim 8, characterized in that, The health food product is an antioxidant solid beverage, prepared from the following weight ratios: 4-6% willow tea melanin masterbatch, 7-9% tea polyphenol masterbatch, 8-12% SOD trienzyme masterbatch, 2-4% ginsenoside masterbatch, 25-32% erythritol, 15-18% fructooligosaccharides, 10-12% inulin, 5-8% resistant dextrin, 3-5% maltodextrin, 2-4% blueberry anthocyanins, 0.2-0.5% curcumin, 0.1-0.3% apigenin, 0.1-0.3% sulforaphane, 0.05-0.1% allicin, 0.5-1% garland chrysanthemum extract, 1-2% creatine, 0.05-0.1% ergothioneine, and 0.1-0.3% polydeoxynucleotides.

11. An application as described in claim 8, characterized in that, Using 800-1200ppb hydrogen-rich water as a carrier, a combination of masterbatch, peptides, sodium hyaluronate, ceramides, niacinamide, and plant extracts is used to prepare serums, creams, ampoules, waters, and lotions.

12. The application according to claim 11, characterized in that, Cosmetics are not limited to the listed raw materials, proportions, dosage forms, and processes.

13. The application according to claim 11 or 12, characterized in that, Using hydrogen-rich water as a solvent, compound masterbatch and food-grade raw materials are used to prepare anti-aging, anti-fatigue, intestinal conditioning, beauty and energy-boosting products; raw materials include but are not limited to ginseng, oyster, cistanche, eucommia, citron, donkey-hide gelatin, rose, wolfberry, longan, red date, astragalus, angelica, green plum, noni fruit, blueberry and mulberry.

14. The application according to claim 13, characterized in that, Oral products are not limited to the listed raw materials, proportions, dosage forms, processes, and application scenarios.

Images