A hair growth stem cell essence product and a preparation method thereof

Abstract

This invention relates to the A61K8 / 18 field, specifically to a hair regrowth stem cell essence product and its preparation method. The raw materials, by weight percentage, include at least 0.01-10% hair regrowth composition, 0.3-1% antioxidant, 0.3-1% anti-inflammatory agent, 5-15% emulsifier, 3-10% solubilizer, and a carrier to make up the balance. The hair regrowth stem cell essence product provided by this invention has excellent therapeutic effects, rapidly promoting stem cell stimulation and cell differentiation, activating hair follicles, and greatly accelerating new hair growth. It prevents hair loss and graying by inducing the expression of hair growth-related growth factors and nourishing hair follicle cells, thus preventing aging. It also protects hair and scalp from free radical damage, maintaining stronger, thicker, and healthier hair and scalp, without any obvious adverse reactions or toxicity.

Description

Technical Field

[0001] This invention relates to the field of A61K8 / 18, specifically to a hair growth stem cell essence product and its preparation method. Background Technology

[0002] Human hair is a keratin-containing, thread-like growth that extends from hair follicles in the skin. The scalp has approximately 110,000 hair follicles, which grow and fall out daily. Hair loss can occur at any age and manifests as a disruption in the balance between hair growth and shedding due to various factors. It is more prevalent in men than women; 40% of men experience noticeable hair loss by age 35, and this figure reaches 65% by age 60. Women are also significantly affected by this process: 50-75% of them experience noticeable hair loss by age 65. Hair loss can have a devastating impact on a person's self-image and emotional well-being.

[0003] There are various hair care products on the market, but their effectiveness is limited. Minoxidil and finasteride, as FDA-approved effective drugs for treating androgenetic alopecia, are disclosed in Chinese patent CN113827608A as a highly effective minoxidil hair growth tincture containing purine nucleosides. While using minoxidil and finasteride as active ingredients, it has some effect, but its side effects are not negligible. For example, minoxidil can cause irritation, dryness, flaking, itching, and redness after use. It also requires a medical prescription and is only effective for a subset of people. Due to hormonal effects, women taking finasteride may increase the risk of male pseudohermaphroditism in their offspring, and may also experience impotence, headaches, dizziness, ejaculation disorders, testicular pain, and erectile dysfunction. Chinese patent CN107157804B discloses a hair growth essence for nourishing the scalp and hair follicles and its preparation method. It uses vitamin B3, allantoin, D-panthenol, vitamin A, and vitamin H as nutritional components. The formula is designed to provide a hair growth essence that achieves a better hair growth conditioning effect without irritating the scalp. However, it is not effective in promoting new hair growth and improving scalp health. Summary of the Invention

[0004] To address the aforementioned problems and shortcomings of existing technologies, this invention provides a hair growth stem cell essence product for rapidly inducing stem cell stimulation and differentiation in dermal and hair follicle cells, promoting new hair growth and improving scalp health.

[0005] The present invention provides a hair growth stem cell essence product, the raw materials of which, by weight percentage, include at least 0.01-10% hair growth composition, 0.3-1% antioxidant, 0.3-1% anti-inflammatory agent, 5-15% emulsifier, 3-10% solubilizer, and the balance being a carrier.

[0006] As a preferred technical solution, the hair growth composition comprises, by weight, at least 0.05-0.3 parts stem cell stimulating protein, 0.1-0.8 parts hydrolyzed rice protein, 0.01-1 parts active protein, 0.01-0.5 parts complex amino acids, and 0.01-0.3 parts hair growth peptide; As a preferred technical solution, the stem cell stimulating protein includes dihydroquercetin-glucoside and epigallocatechin gallate-glucoside; the mass ratio of dihydroquercetin-glucoside and epigallocatechin gallate-glucoside is (0.05-0.2):(0.01-0.1).

[0007] The activation, regeneration, proliferation, and growth of hair follicle cells depend on a variety of factors, one of which is sufficient signals and growth factors that can effectively stimulate and regulate the activation and differentiation of hair follicle stem cells. The inventors studied the role of protruding stem cells, a special type of quiescent hair follicle stem cell that resides in the outer root sheath cells (ORSCs) of the hair follicle during the hair growth cycle. The inventors discovered that ORSCs play a crucial role in initiating the hair growth phase. During their research, they found that a hair growth composition comprising stem cell stimulating protein, hydrolyzed rice protein, potent active protein, complex amino acids, hair growth peptides, carboxymethyl chitosan, and diguanidine tetraphosphate, along with other ingredients in the system, can effectively treat widespread hair loss caused by various factors through different mechanisms. By rapidly inducing stem cell stimulation and differentiation into hair follicle cells, it promotes the regeneration of resting hair follicle cells in the anagen phase and prolongs the anagen phase in the hair cycle, thereby promoting new hair growth, preventing aging, effectively promoting hair regeneration, improving scalp health, inhibiting inflammatory secretions and itching, reducing hair loss, enhancing or restoring hair volume and fullness, increasing hair thickness, improving the general appearance of hair and scalp, while reducing or eliminating scalp inflammation and other allergic reactions, enhancing scalp circulation, and achieving full perfusion of hair follicles from the root.

[0008] Based on the system of this invention, the introduction of dihydroquercetin-glucoside can effectively activate the division of ORSCs, regulate their differentiation characteristics, prevent ORSC apoptosis through the BCL2 activation mechanism, drive ORSCs into the growth phase cycle through the β-catenin activation mechanism, and enhance the metabolism of dermal fibroblasts and human follicular blast cells (HFPDCs).

[0009] Epigallocatechin gallate-glucoside is a therapeutic compound. Based on the system of this invention, by controlling the mass ratio of dihydroquercetin-glucoside to epigallocatechin gallate-glucoside to be (0.05-0.2):(0.01-0.1), the effect of dihydroquercetin-glucoside (DHQG) can be synergistically enhanced, effectively inhibiting the expression of typical inflammatory factors (interleukin-8) in the scalp of subjects with hair loss. The inventors have conducted research on this, and epigallocatechin gallate-glucoside can effectively prevent the effects of dihydrotestosterone (DHT) through the regulatory mechanism of miRNA expression in human dermal cells (HDPC), weakening DHT-mediated apoptosis and growth arrest, reducing intracellular ROS accumulation and senescence, and showing effective antioxidant and anti-inflammatory functions. Through bioinformatics analysis, the inventors confirmed a significant correlation between altered miRNA expression and the protective effect of epigallocatechin gallate-glucoside against DHT. This was manifested in a significant decrease in DHT concentration and a substantial increase in the volume of newly formed, stronger, and thicker hair, covering the balding areas and effectively preventing hair loss. Furthermore, the sonic hedgehog (Shh) and protein kinase B (AKT) signaling pathways are crucial for hair follicle development and growth, as well as the transition between the anagen and telogen phases. The inventors unexpectedly discovered that epigallocatechin gallate-glucoside significantly promotes the activation of the Shh and AKT signaling pathways, contributing to hair follicle cell proliferation and thus promoting rapid hair growth while preventing damage to the scalp and hair cells.

[0010] Hair follicle growth depends on many factors, one of which is the nutrition supplied to the follicles. Blood flow to the scalp plays a crucial role in providing this nutrition. Furthermore, increased blood flow to the scalp tissue enhances the absorption of physiologically functional components throughout the follicles. The stimulation and transformation of active cells by stem cells also depends on blood flow to the tissues. In actual research, the inventors discovered that a hair regrowth stem cell essence product with dihydroquercetin-glucoside and epigallocatechin gallate-glucoside as its main ingredients can significantly increase blood flow. This increases perfusion of the scalp surface tissues (dermis and hair follicle stem cells) through a sufficient supply of nutrients, oxygen, growth factors, and other stimulating molecules. These stimulating molecules, through their pharmacological responses, enhance and / or maintain blood flow to specific tissues (local / systemic), making the provided hair regrowth stem cell essence effective for various hair loss treatments and hair care.

[0011] As a preferred technical solution, the hair growth composition further includes 5-10 parts by weight of carboxymethyl chitosan and 0.2-1.2 parts by weight of diguanidine tetraphosphate.

[0012] Based on the system of this invention, by introducing 5-10 parts of carboxymethyl chitosan and 0.2-1.2 parts of diguanidine tetraphosphate, on the one hand, it improves scalp health by reducing rashes and scalp moisture retention, resisting ultraviolet radiation, cold, heat, infrared rays and other adverse aging stresses, and synergistically increasing and maintaining effective blood circulation in hair follicle cells with other active ingredients, promoting rapid hair growth and improving scalp health; on the other hand, it effectively plays an anti-inflammatory and anti-itch role by reducing inflammatory cell infiltration and controlling the growth of bacteria and fungi, promoting effective prevention of infection and increasing collagen deposition.

[0013] As a preferred technical solution, the antioxidant is one or a combination of several of polyphenols, 3,4-didehydroretinone, lycopene, lutein, retinaldehyde, and carotene; preferably, the carotene is selected from at least one of α-carotene, β-carotene, γ-carotene, and δ-carotene; preferably, the antioxidant is a combination of 3,4-didehydroretinone, lutein, and carotene; the mass ratio of 3,4-didehydroretinone, lutein, and carotene is (1-2):(1.2-2.2):(0.8-1.5).

[0014] Reactive oxygen species (ROS) are a major contributing factor to scalp aging. Exposure to ultraviolet (UV) radiation induces photochemical reactions that occur continuously on the scalp, generating ROS. ROS can damage the scalp, dermis, and hair follicle cells. ROS disrupts the antioxidant defense layer composed of antioxidant enzymes and non-enzymatic antioxidants, shifting the oxidant / antioxidant balance to an oxidative state. This oxidative stress can lead to damage to tissue components, such as lipid peroxidation, protein oxidation, activation of proteases that damage scalp components, chain breaks and abnormal cross-conjugation of collagen and elastin, chain breaks of hyaluronic acid, promotion of melanin synthesis, and DNA oxidation. Furthermore, this damage to scalp and tissue components contributes to various forms of aging and apoptosis of dermal and hair follicle cells. Under the influence of oxidative stress, collagen is broken down, reducing collagen in the scalp. Over time, the ECM around the scalp pores decreases, leading to scalp damage and progressive dermal and hair follicle apoptosis. Based on the system of this invention, by employing an antioxidant composition including 3,4-didehydroretin, lutein, and carotene, the oxidation process is effectively inhibited. By scavenging various forms of reactive oxygen species that are absorbed into the cell membrane and neutralizing oxygen free radicals, the system protects hair follicles from oxidative damage, including inflammation, enables an effective immune response, eradicates infectious microorganisms, improves hair growth and scalp health, and prevents or eliminates hair loss, providing effective therapeutic and cosmetic benefits, thereby improving the patient's psychological and physical health.

[0015] As a preferred technical solution, the anti-inflammatory agent is one of bisabolol, nicotinamide, and ectoine; preferably, the anti-inflammatory agent is bisabolol.

[0016] As a preferred technical solution, the emulsifier is an amino acid surfactant. Preferably, the amino acid surfactant is selected from at least one of sodium lauroyl glutamate, sodium cocoyl glutamate, disodium cocoyl glutamate, potassium cocoyl glutamate, and TEA salt of cocoyl glutamate; preferably, the amino acid surfactant is a combination of sodium lauroyl glutamate and disodium cocoyl glutamate; the mass ratio of sodium lauroyl glutamate to disodium cocoyl glutamate is (1-3):(1-2).

[0017] As a preferred technical solution, the co-solvent is at least one of glycerol, ethoxydiethylene glycol, butanediol, propylene glycol, triglycerides, and 1,2-triglycerides; preferably, the co-solvent is glycerol and ethoxydiethylene glycol; the mass ratio of glycerol to ethoxydiethylene glycol is (4-6):(1-2).

[0018] As a preferred technical solution, the carrier is deionized water.

[0019] Another aspect of the present invention provides a method for preparing a hair growth stem cell essence product, comprising at least the following steps: (1) Add the carrier, cosolvent, antioxidant, anti-inflammatory agent and emulsifier to a mixing container and stir to obtain mixture A; (2) Add the hair growth composition to the above mixture A and stir until uniform to obtain the hair growth stem cell essence product.

[0020] Beneficial effects: 1. This invention provides a hair growth stem cell essence product for rapidly inducing stem cell stimulation and differentiation in dermal and hair follicle cells, promoting new hair growth and improving scalp health.

[0021] 2. The hair growth stem cell essence product provided by this invention can effectively activate hair stem cells, prevent hair follicle aging, restore the vitality of hair follicles, prevent scalp and hair aging, enhance the colony formation efficiency of epidermal stem cells and dermis, promote the regeneration of new hair follicles, maintain the characteristics of dermal stem cells and promote new hair growth, maintain the regenerative potential of keratinocytes in hair follicles, prolong lifespan / growth, and prolong the growth phase and shorter resting phase of isolated hair follicles. 3. The hair growth stem cell essence product provided by this invention can increase the volume and thickness of hair after use, and minimize hair loss on the scalp; at the same time, it protects the scalp, eliminates the harmful effects of infectious microorganisms, balances the normal flora, nourishes hair follicles, regulates sebum secretion, and prevents premature aging.

[0022] 4. The hair growth stem cell essence product provided by this invention has low dependence and can be used by people with various types of hair loss. Detailed Implementation

[0023] Example 1 In one aspect, Embodiment 1 of the present invention provides a hair growth stem cell essence product, the raw materials of which, by weight percentage, include 6.8% hair growth composition, 0.5% antioxidant, 0.5% anti-inflammatory agent, 10% emulsifier, 8% solubilizer, and the balance being a carrier.

[0024] By weight, the hair growth composition comprises at least 0.26 parts of stem cell stimulating protein, 0.8 parts of hydrolyzed rice protein, 0.05 parts of active protein, 0.04 parts of complex amino acids, and 0.05 parts of hair growth peptide; The stem cell stimulating protein includes dihydroquercetin-glucoside and epigallocatechin gallate-glucoside; the mass ratio of dihydroquercetin-glucoside and epigallocatechin gallate-glucoside is 0.2:0.06.

[0025] The compound amino acids were sourced from Shanghai Baihaobo Chemical Co., Ltd.

[0026] The hair growth composition further comprises, by weight, 5 parts carboxymethyl chitosan and 0.6 parts diguanidine tetraphosphate.

[0027] The antioxidant is a combination of 3,4-didehydroretin, lutein, and carotene; the mass ratio of 3,4-didehydroretin, lutein, and carotene is 1.5:1.8:1.2.

[0028] The anti-inflammatory agent is bisabolol.

[0029] The emulsifier is an amino acid surfactant. The amino acid surfactant is a combination of sodium lauroyl glutamate and disodium cocoyl glutamate; the mass ratio of sodium lauroyl glutamate to disodium cocoyl glutamate is 2:1.5.

[0030] The cosolvent is glycerol and ethoxydiethylene glycol; the mass ratio of glycerol to ethoxydiethylene glycol is 5:2.

[0031] The carrier is deionized water.

[0032] Another aspect of Embodiment 1 of the present invention provides a method for preparing a hair regrowth stem cell essence product, comprising the following steps: (1) Add the carrier, cosolvent, antioxidant, anti-inflammatory agent and emulsifier to a mixing container and stir to obtain mixture A; (2) Add the hair growth composition to the above mixture A and stir until uniform to obtain the hair growth stem cell essence product.

[0033] Example 2 In one aspect, Embodiment 2 of the present invention provides a hair growth stem cell essence product, the raw materials for which, by weight percentage, include 6.8% hair growth composition, 0.5% antioxidant, 0.5% anti-inflammatory agent, 10% emulsifier, 8% solubilizer, and the balance being a carrier.

[0034] By weight, the hair growth composition comprises at least 0.26 parts of stem cell stimulating protein, 0.8 parts of hydrolyzed rice protein, 0.05 parts of active protein, 0.04 parts of complex amino acids, and 0.05 parts of hair growth peptide; The stem cell stimulating protein includes dihydroquercetin-glucoside and epigallocatechin gallate-glucoside; the mass ratio of dihydroquercetin-glucoside and epigallocatechin gallate-glucoside is 0.2:0.06.

[0035] The compound amino acids were sourced from Shanghai Baihaobo Chemical Co., Ltd.

[0036] The hair growth composition further comprises, by weight, 5 parts carboxymethyl chitosan and 0.6 parts diguanidine tetraphosphate.

[0037] The antioxidant is a combination of 3,4-didehydroretin, lutein, and carotene; the mass ratio of 3,4-didehydroretin, lutein, and carotene is 1.5:1.8:1.2.

[0038] The anti-inflammatory agent is bisabolol.

[0039] The emulsifier is an amino acid surfactant. The amino acid surfactant is a combination of sodium lauroyl glutamate and disodium cocoyl glutamate; the mass ratio of sodium lauroyl glutamate to disodium cocoyl glutamate is 2:1.5.

[0040] The cosolvent is glycerol and ethoxydiethylene glycol; the mass ratio of glycerol to ethoxydiethylene glycol is 5:2.

[0041] The carrier is deionized water.

[0042] Another aspect of the present invention, Embodiment 2, provides a method for preparing a hair regrowth stem cell essence product, comprising the following steps: (1) Add the carrier, cosolvent, antioxidant, anti-inflammatory agent and emulsifier to a mixing container and stir to obtain mixture A; (2) Add the hair growth composition to the above mixture A and stir until uniform to obtain the hair growth stem cell essence product.

[0043] Example 3 In one aspect, Embodiment 3 of the present invention provides a hair growth stem cell essence product, the raw materials for which, by weight percentage, include 6.8% hair growth composition, 0.5% antioxidant, 0.5% anti-inflammatory agent, 10% emulsifier, 8% solubilizer, and the balance being a carrier.

[0044] By weight, the hair growth composition comprises at least 0.26 parts of stem cell stimulating protein, 0.8 parts of hydrolyzed rice protein, 0.05 parts of active protein, 0.04 parts of complex amino acids, and 0.05 parts of hair growth peptide; The stem cell stimulating protein includes dihydroquercetin-glucoside and epigallocatechin gallate-glucoside; the mass ratio of dihydroquercetin-glucoside and epigallocatechin gallate-glucoside is 0.2:0.06.

[0045] The compound amino acids were sourced from Shanghai Baihaobo Chemical Co., Ltd.

[0046] The hair growth composition further comprises, by weight, 5 parts carboxymethyl chitosan and 0.6 parts diguanidine tetraphosphate.

[0047] The antioxidant is a combination of 3,4-didehydroretin, lutein, and carotene; the mass ratio of 3,4-didehydroretin, lutein, and carotene is 1.5:1.8:1.2.

[0048] The anti-inflammatory agent is bisabolol.

[0049] The emulsifier is an amino acid surfactant. The amino acid surfactant is a combination of sodium lauroyl glutamate and disodium cocoyl glutamate; the mass ratio of sodium lauroyl glutamate to disodium cocoyl glutamate is 2:1.5.

[0050] The cosolvent is glycerol and ethoxydiethylene glycol; the mass ratio of glycerol to ethoxydiethylene glycol is 5:2.

[0051] The carrier is deionized water.

[0052] Example 3 of the present invention provides a method for preparing a hair growth stem cell essence product, comprising the following steps: (1) Add the carrier, cosolvent, antioxidant, anti-inflammatory agent and emulsifier to a mixing container and stir to obtain mixture A; (2) Add the hair growth composition to the above mixture A and stir until uniform to obtain the hair growth stem cell essence product.

[0053] Performance testing methods 1. Cosmetic safety and efficacy testing Based on the technical requirements of the Commission and the simple acceptance (risk-sharing) judgment rule of our laboratory GDYJJC-SOP-01-031 "Conformity Statement and Operating Procedures", the hair regrowth stem cell essence product provided in Example 3 was tested for cosmetic safety and efficacy. The test report is shown in Table 1.

[0054]

[0055] 2. Efficacy Test: (1) Test the effects of the hair growth stem cell essence product provided in Example 3 on hair growth, scalp health and prevention of baldness in individuals with severe hair loss.

[0056] 1) A 34-year-old male patient used the product provided in Example 3, applying it twice daily to the balding areas of his scalp for four consecutive months. Photorecognition of the patient's scalp revealed a significant increase in new hair follicles and new hair tips. These follicles were stronger and thicker, increasing the coverage of the affected area of ​​the scalp by increasing hair volume.

[0057] 2) A 55-year-old woman used the product provided in Example 3 and applied it twice a day to the balding areas of her scalp. After two months of continuous use, the patient reported that her scalp and head had improved, her hair had grown and increased in volume, and her scalp felt healthy.

[0058] 3) A 61-year-old male used the product provided in Example 3, applying it twice daily to the balding areas of his scalp. After two months of continuous use, the patient reported an increase in new hair follicles and new hair growth, which were stronger and thicker, resulting in increased scalp coverage.

[0059] (2) Test the dependence and effects of individuals with severe hair loss on the hair growth stem cell essence products provided in Examples 1, 2 and 3.

[0060] 1) Ten subjects with severe hair loss used the hair growth stem cell essence products provided in Example 2 (5 subjects) and Example 3 (5 subjects) twice a day on the hair loss area of ​​the scalp for 8 consecutive weeks. After stopping use, hair growth still existed and continued, proving that the product has low dependence.

[0061] 2) Two female subjects with severe hair loss and thinning hair used the product provided in Example 3 for 6 weeks, applying it twice a day to the hair loss area of ​​the scalp. Photosensitive physical examination showed that the subjects had a significant increase in new hair follicles and a substantial increase in hair volume.

[0062] 3) Eight male subjects with varying degrees of hair loss used the product provided in Example 3. They applied the product to the balding areas of the scalp twice a day for four consecutive weeks. Photosensitive physical examination showed that the subjects' new terminal hair growth exhibited a significant peach effect, increasing scalp coverage and resulting in stronger and thicker hair.

Claims

1. A hair regrowth stem cell essence product, characterized in that, The raw materials used in its preparation, by weight percentage, include at least 0.01-10% hair growth composition, 0.3-1% antioxidant, 0.3-1% anti-inflammatory agent, 5-15% emulsifier, 3-10% cosolvent, and the balance being carrier.

2. The hair regrowth stem cell essence product according to claim 1, characterized in that, By weight, the hair growth composition comprises at least 0.05-0.3 parts stem cell stimulating protein, 0.1-0.8 parts hydrolyzed rice protein, 0.01-1 parts active protein, 0.01-0.5 parts complex amino acids, and 0.01-0.3 parts hair growth peptide.

3. The hair regrowth stem cell essence product according to claim 2, characterized in that, The stem cell stimulating proteins include dihydroquercetin-glucoside and epigallocatechin gallate-glucoside.

4. The hair regrowth stem cell essence product according to claim 2, characterized in that, The hair growth composition further comprises, by weight, 5-10 parts carboxymethyl chitosan and 0.2-1.2 parts diguanidine tetraphosphate.

5. A hair regrowth stem cell essence product according to claim 4, characterized in that, The antioxidant is one or a combination of several of the following: polyphenols, 3,4-didehydroretinone, lycopene, lutein, retinaldehyde, and carotene.

6. A hair regrowth stem cell essence product according to claim 5, characterized in that, The anti-inflammatory agent is one of bisabolol, nicotinamide, and ectoine.

7. A hair regrowth stem cell essence product according to claim 6, characterized in that, The emulsifier is an amino acid surfactant.

8. A hair regrowth stem cell essence product according to claim 7, characterized in that, The amino acid surfactant is selected from at least one of sodium lauroyl glutamate, sodium cocoyl glutamate, disodium cocoyl glutamate, potassium cocoyl glutamate, and TEA salt of cocoyl glutamate.

9. A hair regrowth stem cell essence product according to claim 8, characterized in that, The cosolvent is at least one of glycerol, ethoxydiethylene glycol, butanediol, propylene glycol, triglycerides, and 1,2-triglycerides.

10. A method for preparing a hair regrowth stem cell essence product according to any one of claims 1-9, characterized in that, At least the following steps are included: (1) Add the carrier, cosolvent, antioxidant, anti-inflammatory agent and emulsifier to a mixing container and stir to obtain mixture A; (2) Add the hair growth composition to the above mixture A and stir until uniform to obtain the hair growth stem cell essence product.

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