A post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the uterus and ovaries

Abstract

This invention discloses a post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries, which, by mass percentage, consists of the following components: 60.0%–75.0% purified water, 3.0%–6.0% lactic acid bacteria fermentation lysate, 2.0%–4.0% L-arabinose, 1.5%–3.5% double-petaled red rose extract, 1.0%–3.0% cordyceps extract, 1.0%–2.5% γ-aminobutyric acid, 1.5%–3.0% red ginseng concentrate, 1.0%–2.5% cranberry fruit powder, 0.5%–2.0% turmeric extract, and vitamin C. The ingredients include 0.5%–1.5%, frog polypeptide powder 1.0%–3.0%, carob extract 1.0%–2.5%, roselle extract 0.5%–2.0%, tremella polysaccharide 0.5%–2.0%, and yeast β-glucan 0.5%–2.0%; the sum of the mass percentages of each ingredient is 100%. The ingredients of this invention work synergistically to exert multiple effects such as endocrine regulation, uterine and ovarian care, pelvic floor muscle repair, and skin firming and moisturizing, solving the problems of single efficacy and poor synergy in existing preparations.

Description

Technical Field

[0001] This invention belongs to the field of compound health care preparation technology, specifically a post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the uterus and ovaries. Background Technology

[0002] The female endocrine system is a vital system for maintaining bodily physiological functions. Endocrine disorders can easily lead to a series of problems such as uterine and ovarian dysfunction, pelvic floor muscle relaxation, skin aging, and vaginal dryness, seriously affecting women's physical and mental health and quality of life. Currently, there are many preparations on the market targeting female endocrine regulation and uterine and ovarian care, but most suffer from single-ingredient formulations and limited efficacy. For example, some only target uterine and ovarian care, failing to address pelvic floor muscle repair and skin condition improvement. Some preparations use unreasonable manufacturing processes, leading to the loss of active ingredients and reduced bioactivity, thus affecting their effectiveness. Furthermore, existing preparations also suffer from poor compatibility and weak synergistic effects among their active ingredients, making it difficult to achieve comprehensive care with multiple targets and effects.

[0003] Postbiotics, as a mixture of probiotic metabolites and bacterial components, have the effects of regulating intestinal flora, improving immunity, and regulating endocrine function. They are stable, have no safety risks associated with live bacteria, and have broad application prospects in health supplements. Plant extracts, rich in polyphenols, flavonoids, saponins, and other active ingredients, can nourish the uterus and ovaries and regulate blood circulation. Polypeptides, such as frog polypeptides, can repair tissues and enhance vitality. Functional polysaccharides can enhance immunity and improve skin hydration. Currently, there are no reports on the scientific compounding of postbiotics, plant extracts, animal polypeptides, functional polysaccharides, and various functional additives, combined with a proprietary liquid-solid fermentation process, to prepare a compound preparation that combines endocrine regulation, uterine and ovarian care, pelvic floor muscle repair, and skin firming and hydration. Therefore, developing a synergistic, technologically advanced, and comprehensive women's health care compound preparation is of significant practical importance.

[0004] Based on this, a post-biotic plant polypeptide compound preparation for regulating endocrine function and nourishing the uterus and ovaries was designed. Summary of the Invention

[0005] In view of the above situation and to overcome the defects of the prior art, the present invention provides a post-biotic plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries, which effectively solves the problems mentioned in the background.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries, comprising, by mass percentage: 60.0%–75.0% purified water, 3.0%–6.0% lactic acid bacteria fermentation lysate, 2.0%–4.0% L-arabinose, 1.5%–3.5% double-petaled red rose extract, 1.0%–3.0% cordyceps extract, 1.0%–2.5% γ-aminobutyric acid, 1.5%–3.0% red ginseng concentrate, 1.0%–2.5% cranberry fruit powder, 0.5%–2.0% turmeric extract, and vitamin C. 0.5%–1.5%, frog polypeptide powder 1.0%–3.0%, carob extract 1.0%–2.5%, roselle extract 0.5%–2.0%, tremella polysaccharide 0.5%–2.0%, yeast β-glucan 0.5%–2.0%; the sum of the mass percentages of all components is 100%.

[0007] Preferably, the preferred formula, by mass percentage, is: 68.5% purified water, 4.5% lactic acid bacteria fermentation lysate, 3.0% L-arabinose, 2.5% double-petaled red rose extract, 2.0% cordyceps extract, 1.8% γ-aminobutyric acid, 2.0% red ginseng concentrate, 1.8% cranberry fruit powder, 1.2% turmeric extract, 1.0% vitamin C, 2.0% forest frog polypeptide powder, 1.8% carob extract, 1.2% roselle extract, 1.2% tremella polysaccharide, and 1.5% yeast β-glucan.

[0008] A method for preparing a post-biotic plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries includes the following steps: S1. Raw material pretreatment: Double-petaled red rose extract, Cordyceps militaris extract, turmeric extract, carob extract, and roselle extract were placed in a vacuum drying oven and dried for 2-4 hours at a temperature of 45℃~55℃ and a vacuum degree of -0.08MPa~-0.10MPa. After drying, they were pulverized by an ultra-micro pulverizer and passed through an 800-1000 mesh sieve to obtain fine plant powder for later use. Forest frog polypeptide powder, Tremella fuciformis polysaccharide, and yeast β-glucan were placed in a constant temperature desiccator and dried for 1-2 hours at a temperature of 35℃~40℃ to remove surface moisture and sealed for later use. Red ginseng concentrate was pre-cooled in a refrigerated environment at 4℃~8℃ for later use. S2. Liquid-solid fermentation pretreatment: Mix 50% to 60% of the lactic acid bacteria fermentation lysate with purified water and place it in a fermenter. Adjust the temperature to 30℃ to 37℃ and the pH value to 6.0 to 7.0. Add 0.05% to 0.10% of the total mass of the mixture as a compound fermentation aid. Stir and ferment at 100 to 150 rpm for 8 to 12 hours to obtain the fermentation base liquid. After fermentation, pasteurize at 60℃ to 65℃ for 20 to 30 minutes and cool to room temperature. S3. Gradient dissolution of active ingredients: Add the pretreated frog polypeptide powder, tremella polysaccharide, and yeast β-glucan to the remaining purified water, and stir to dissolve for 30 min to 60 min at a temperature of 40℃~50℃ and a speed of 80 r / min~120 r / min to obtain a polysaccharide polypeptide solution; add L-arabinose, γ-aminobutyric acid, and vitamin C to the solution, and continue stirring at the same speed for 15 min to 30 min to obtain the basic active solution; S4. Combining plant components with fermentation broth: Add the plant powder obtained in step S1 to the fermentation base broth in step S2, and stir and disperse for 40 min to 60 min at a temperature of 25℃~30℃ and a rotation speed of 60 r / min~100 r / min. Then, use ultrasonic-assisted extraction with an ultrasonic power of 200 W~300 W and an ultrasonic time of 20 min~30 min to obtain the plant fermentation compound broth. S5. Total liquid preparation and homogenization: Mix the basic active liquid from step S3 and the plant fermentation compound liquid from step S4, add the pre-cooled red ginseng concentrate and cranberry powder, and stir and mix for 60 min to 90 min at a temperature of 20℃~25℃ and a speed of 120 r / min~180 r / min to obtain the initial liquid preparation; send the initial liquid preparation into a high-pressure homogenizer and homogenize 2 to 3 times at a pressure of 40MPa~60MPa and a temperature of 20℃~25℃, with each homogenization lasting 10 min to 15 min; S6. Filtration and finished product preparation: The homogenized initial solution is vacuum filtered through a 0.22μm microporous membrane to remove insoluble impurities and obtain filtrate; the filtrate is placed in a sterile filling machine, filled and sealed under sterile conditions, and after passing light inspection, the post-natal plant polypeptide endocrine regulation and ovarian care compound preparation is obtained.

[0009] Preferably, the compound fermentation aid in step S2 is a mixture of maltodextrin and fructooligosaccharides in a mass ratio of 1:1 to 2:1.

[0010] Preferably, in step S4, the ultrasonic-assisted extraction uses intermittent ultrasound, with 5 minutes of ultrasound followed by a 2-minute pause, and the operation is repeated until the set ultrasound time is reached.

[0011] Preferably, the compound formulation is used in the preparation of oral liquids, gels, lyophilized powders for the treatment of female endocrine regulation, uterine and ovarian care, pelvic floor muscle repair, or skin firming and hydration.

[0012] Compared with the prior art, the beneficial effects of the present invention are: 1. The innovative compound formulation of this invention scientifically combines post-biotics, plant extracts, animal polypeptides, functional polysaccharides and various functional additives for the first time. Each component is precisely proportioned by mass percentage to synergistically exert multiple effects such as endocrine regulation, uterine and ovarian care, pelvic floor muscle repair and skin firming and moisturizing, thus solving the problems of single efficacy and poor synergy of existing formulations. 2. The preparation method of the present invention is innovative. It adopts a combination of liquid-solid segmented fermentation process with gradient dissolution, ultrasonic-assisted extraction, low-temperature compounding and high-pressure homogenization, which maximizes the preservation of the bioactivity of each active ingredient, while improving the compatibility of each ingredient and the stability of the formulation. The preparation process parameters are controllable and the operation is simple, making it suitable for large-scale industrial production. 3. The compound formulation of the present invention uses purified water as a base, contains no harmful additives, and all components are safe and mild, suitable for a wide range of people. It can be prepared into a variety of health care products such as oral liquids, gels, and freeze-dried powders, with rich application scenarios and broad market prospects. Detailed Implementation

[0013] The technical solution of the present invention will be clearly and completely described below with reference to specific embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0014] This invention provides a post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries, comprising the following components by mass percentage: 60.0%–75.0% purified water, 3.0%–6.0% lactic acid bacteria fermentation lysate, 2.0%–4.0% L-arabinose, 1.5%–3.5% double-petaled red rose extract, 1.0%–3.0% cordyceps extract, 1.0%–2.5% γ-aminobutyric acid, 1.5%–3.0% red ginseng concentrate, 1.0%–2.5% cranberry fruit powder, 0.5%–2.0% turmeric extract, and vitamin C. 0.5%–1.5%, frog (snow frog) polypeptide powder 1.0%–3.0%, carob extract 1.0%–2.5%, roselle extract 0.5%–2.0%, tremella polysaccharide 0.5%–2.0%, yeast β-glucan 0.5%–2.0%; the sum of the mass percentages of all components is 100%.

[0015] Preferably, the optimal formulation of this compound preparation is as follows: 68.5% purified water, 4.5% lactic acid bacteria fermentation lysate, 3.0% L-arabinose, 2.5% double-petaled red rose extract, 2.0% cordyceps militaris extract, 1.8% γ-aminobutyric acid, 2.0% red ginseng concentrate, 1.8% cranberry fruit powder, 1.2% turmeric extract, 1.0% vitamin C, 2.0% forest frog polypeptide powder, 1.8% carob extract, 1.2% roselle extract, 1.2% tremella polysaccharide, and 1.5% yeast β-glucan.

[0016] The efficacy and synergistic effects of the components of this invention are as follows: Lactic acid bacteria fermentation lysate: As a core postbiotic component, it regulates the balance of intestinal flora, regulates endocrine homeostasis through the gut-ovarian axis, and enhances the body's immunity, laying the foundation for uterine and ovarian care; Forest frog polypeptide powder: rich in small molecule active polypeptides, which can repair pelvic floor muscle fibers, improve the elasticity and contractility of pelvic floor muscles, and at the same time repair damaged tissues around the uterus and ovaries, and improve tissue vitality. Red ginseng concentrate and cordyceps militaris extract: work synergistically to replenish qi and blood, nourish the uterus and ovaries, enhance ovarian function, regulate estrogen levels, and improve various discomforts caused by endocrine disorders; Double-petaled red rose extract, hibiscus extract, and carob extract: rich in flavonoids and polyphenols, which regulate blood and qi, soothe endocrine system, improve vaginal mucosa condition, and enhance hydration. Tremella polysaccharide and yeast β-glucan: work synergistically to moisturize and firm, improve the hydration and firmness of the skin and mucous membranes, enhance immunity, and assist in the care of the uterus and ovaries; Gamma-aminobutyric acid (GABA): Soothes nerves, improves mood problems caused by endocrine disorders, and indirectly regulates endocrine homeostasis; L-Arabinose: Regulates intestinal metabolism, works synergistically with lactic acid bacteria fermentation lysates to improve the intestinal environment, while inhibiting excessive sugar absorption and maintaining metabolic balance. Turmeric extract, cranberry powder, and vitamin C: These ingredients have antioxidant and anti-inflammatory effects, scavenge free radicals, and protect ovarian tissue from oxidative damage. At the same time, vitamin C can promote collagen synthesis and assist in the repair of pelvic floor muscles and skin.

[0017] The various ingredients work together to achieve a comprehensive effect of "internal regulation and external nourishment" by targeting multiple points such as intestinal regulation, endocrine regulation, tissue repair, and antioxidant moisturizing, thus solving the problem of single efficacy in existing formulations.

[0018] The present invention also provides a method for preparing the above-mentioned compound formulation, comprising the following steps: S1. Raw material pretreatment Extracts of double-petaled red rose, cordyceps militaris, turmeric, carob, and roselle were placed in a vacuum drying oven and dried for 2-4 hours at 45℃-55℃ and -0.08MPa--0.10MPa. After drying, the powders were pulverized using an ultra-micro pulverizer and passed through an 800-1000 mesh sieve to obtain fine plant powders for later use. Frog polypeptide powder, tremella polysaccharide, and yeast β-glucan were placed in a constant temperature desiccator and dried for 1-2 hours at 35℃-40℃ to remove surface moisture and then sealed for later use. Red ginseng concentrate was pre-cooled in a refrigerated environment at 4℃-8℃ for later use.

[0019] The purpose of pretreatment is to remove free water from plant extracts, and to improve their dispersibility and solubility through ultra-fine pulverization; to dry peptides and polysaccharides at low temperatures to prevent denaturation and inactivation; and to pre-cool red ginseng concentrate to prevent the decomposition of active ingredients due to excessively high temperatures during subsequent compounding.

[0020] S2, Liquid-Solid Fermentation Pretreatment Mix 50%–60% of the lactic acid bacteria fermentation lysate with purified water and place it in a fermenter. Adjust the temperature to 30℃–37℃ and the pH to 6.0–7.0. Add 0.05%–0.10% of the total mass of the mixture of compound fermentation aid (maltodextrin and fructooligosaccharides mixed at a mass ratio of 1:1–2:1). Stir and ferment at 100–150 r / min for 8–12 hours to obtain the fermentation base liquid. After fermentation, pasteurize at 60℃–65℃ for 20–30 minutes and cool to room temperature.

[0021] Key process points: The temperature and pH value provide a suitable environment for the stable performance of lactic acid bacteria metabolites. The compound fermentation aid improves the stability of the fermentation broth and the retention rate of active ingredients. The pasteurization adopts a low temperature and short time method, which kills miscellaneous bacteria while avoiding the loss of metabiotic active ingredients.

[0022] S3, Gradient dissolution of active ingredients Pretreated frog polypeptide powder, tremella polysaccharide, and yeast β-glucan were added to the remaining purified water and stirred at 40℃~50℃ and 80r / min~120r / min for 30min~60min to obtain a polysaccharide polypeptide solution. L-arabinose, γ-aminobutyric acid, and vitamin C were added to the solution and stirred at the same speed for 15min~30min to obtain the basic active solution.

[0023] The advantages of gradient dissolution: It dissolves polysaccharides and peptides with relatively poor solubility first, and then dissolves sugars, amino acids and vitamins that are easily soluble, avoiding the problems of agglomeration and insufficient dissolution caused by the simultaneous addition of ingredients, and improving the compatibility of each ingredient.

[0024] S4, Plant-based ingredients combined with fermentation broth The plant powder obtained in step S1 is added to the fermentation base liquid in step S2 and stirred and dispersed for 40 min to 60 min at a temperature of 25℃~30℃ and a speed of 60 r / min~100 r / min. Then, ultrasonic-assisted extraction is performed (power 200W~300W, time 20 min~30 min, intermittent ultrasound: 5 min ultrasound, 2 min pause) to obtain the plant fermentation compound liquid.

[0025] The role of ultrasound-assisted extraction: By utilizing the cavitation effect of ultrasound, the structure of plant cells is broken, allowing the active ingredients in the plant powder to be fully released into the fermentation base liquid, and fully combined with the post-genetic components to enhance the synergistic effect; intermittent ultrasound avoids excessive local temperature that could lead to component inactivation.

[0026] S5, Total Liquid Blending and Homogenization Mix the basic active liquid from step S3 and the plant fermentation compound liquid from step S4, add the pre-cooled red ginseng concentrate and cranberry powder, and stir and mix for 60 min to 90 min at a temperature of 20℃~25℃ and a speed of 120 r / min~180 r / min to obtain the initial solution; send the initial solution into a high-pressure homogenizer and homogenize it 2 to 3 times at a pressure of 40MPa~60MPa and a temperature of 20℃~25℃, with each homogenization lasting 10 min to 15 min.

[0027] Low-temperature compounding + high-pressure homogenization: The low-temperature environment effectively preserves the bioactivity of all active ingredients, while high-pressure homogenization makes the formulation system more uniform and stable, avoids component stratification, and improves the storage stability and user experience of the formulation.

[0028] S6. Filtration and Finished Product Preparation The homogenized initial solution was vacuum filtered through a 0.22μm microporous membrane to remove insoluble impurities and obtain the filtrate. The filtrate was placed in a sterile filling machine, filled and sealed under sterile conditions, and after passing the light inspection, the post-natal plant polypeptide endocrine regulation and ovarian care compound preparation was obtained.

[0029] Example 1: A post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries, comprising the following components by mass percentage: purified water 60.0%, lactic acid bacteria fermentation lysate 6.0%, L-arabinose 4.0%, double-petaled red rose extract 3.5%, cordyceps militaris extract 3.0%, γ-aminobutyric acid 2.5%, red ginseng concentrate 3.0%, cranberry fruit powder 2.5%, turmeric extract 2.0%, vitamin C 1.5%, forest frog polypeptide powder 3.0%, carob extract 2.5%, roselle extract 2.0%, tremella polysaccharide 2.0%, and yeast β-glucan 2.0%.

[0030] Its preparation method includes the following steps: S1. Raw material pretreatment: Double-petaled red rose extract, Cordyceps militaris extract, turmeric extract, carob extract, and roselle extract were dried at 45℃ and vacuum degree -0.08MPa for 4 hours, and then ultra-finely pulverized through an 800-mesh sieve; Forest frog polypeptide powder, Tremella fuciformis polysaccharide, and yeast β-glucan were dried at 35℃ for 2 hours; Red ginseng concentrate was pre-cooled at 4℃ for later use. S2, Liquid-solid fermentation pretreatment: Take the lactic acid bacteria fermentation lysate and mix it with 60% purified water, adjust the temperature to 30℃ and the pH value to 6.0, add 0.05% compound fermentation aid, which is a mixture of maltodextrin and fructooligosaccharide in a mass ratio of 1:1, stir and ferment at 100r / min for 12h to obtain fermentation base liquid, after fermentation is completed, pasteurize at 60℃ for 30min, and cool naturally to room temperature; S3. Gradient dissolution of active ingredients: Add the remaining 40% purified water to the frog polypeptide powder, tremella polysaccharide, and yeast β-glucan, and stir at 40℃ and 80r / min for 60min to obtain a polysaccharide polypeptide solution; add L-arabinose, γ-aminobutyric acid, and vitamin C to the polysaccharide polypeptide solution, and continue stirring at 80r / min for 30min to obtain the basic active solution; S4. Compounding of plant components with fermentation broth: Fine powders of double-petaled red rose extract, cordyceps militaris extract, turmeric extract, carob extract, and roselle extract are added to the fermentation base broth and stirred and dispersed at 25℃ and 60r / min for 60min. Then, ultrasonic-assisted extraction is performed with an ultrasonic power of 200W and an ultrasonic time of 30min. Intermittent ultrasonication is used, with ultrasonication for 5min followed by a 2min pause. The cycle is repeated until the set ultrasonic time is reached to obtain the plant fermentation compound broth. S5. Total liquid preparation and homogenization: Mix the basic active liquid and the plant fermentation compound liquid, add the pre-cooled red ginseng concentrate and cranberry fruit powder, and stir and mix at 20℃ and 120r / min for 90min to obtain the initial liquid preparation; send the initial liquid preparation into a high-pressure homogenizer and homogenize it 3 times at 40MPa pressure and 20℃ temperature, each time for 15min; S6. Filtration and finished product preparation: The homogenized initial solution is vacuum filtered through a 0.22μm microporous membrane to remove insoluble impurities and obtain filtrate. The filtrate is placed in an aseptic filling machine and filled and sealed under aseptic conditions. After passing the light inspection, the post-natal plant polypeptide regulation endocrine and ovarian care compound preparation is obtained, which can be prepared into oral liquid, gel or lyophilized powder dosage form according to needs.

[0031] Example 2 A post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries, with the following preferred formula by mass percentage: 68.5% purified water, 4.5% lactic acid bacteria fermentation lysate, 3.0% L-arabinose, 2.5% double-petaled red rose extract, 2.0% cordyceps militaris extract, 1.8% γ-aminobutyric acid, 2.0% red ginseng concentrate, 1.8% cranberry fruit powder, 1.2% turmeric extract, 1.0% vitamin C, 2.0% forest frog polypeptide powder, 1.8% carob extract, 1.2% roselle extract, 1.2% tremella polysaccharide, and 1.5% yeast β-glucan.

[0032] Its preparation method includes the following steps: S1. Raw material pretreatment: Double-petaled red rose extract, Cordyceps militaris extract, turmeric extract, carob extract, and roselle extract were dried at 50℃ and vacuum degree -0.09MPa for 3 hours, and then ultra-finely pulverized through a 900-mesh sieve; Forest frog polypeptide powder, Tremella fuciformis polysaccharide, and yeast β-glucan were dried at 38℃ for 1.5 hours; Red ginseng concentrate was pre-cooled at 6℃ for later use. S2, Liquid-solid fermentation pretreatment: Take the lactic acid bacteria fermentation lysate and mix it with 55% purified water, adjust the temperature to 35℃ and the pH value to 6.5, add 0.08% compound fermentation aid, which is a mixture of maltodextrin and fructooligosaccharide in a mass ratio of 1.5:1, stir and ferment at 120r / min for 10h to obtain fermentation base liquid, after fermentation is completed, pasteurize at 63℃ for 25min, and cool naturally to room temperature; S3. Gradient dissolution of active ingredients: Add the remaining 45% purified water to the frog polypeptide powder, tremella polysaccharide, and yeast β-glucan, and stir at 45℃ and 100 r / min for 45 min to obtain a polysaccharide polypeptide solution; add L-arabinose, γ-aminobutyric acid, and vitamin C to the polysaccharide polypeptide solution, and continue stirring at 100 r / min for 20 min to obtain the basic active solution; S4. Compounding of plant components with fermentation broth: Fine powders of double-petaled red rose extract, cordyceps militaris extract, turmeric extract, carob extract, and roselle extract are added to the fermentation base broth and stirred and dispersed at 28℃ and 80r / min for 50min. Then, ultrasonic-assisted extraction is performed with an ultrasonic power of 250W and an ultrasonic time of 25min. Intermittent ultrasonication is used, with ultrasonication for 5min followed by a 2min pause. The cycle is repeated until the set ultrasonic time is reached to obtain the plant fermentation compound broth. S5. Total liquid preparation and homogenization: Mix the basic active liquid and the plant fermentation compound liquid, add the pre-cooled red ginseng concentrate and cranberry powder, and stir and mix at 23℃ and 150r / min for 75min to obtain the initial liquid preparation; send the initial liquid preparation into a high-pressure homogenizer and homogenize twice at 50MPa pressure and 23℃ temperature, each time for 12min. S6. Filtration and finished product preparation: The homogenized initial solution is vacuum filtered through a 0.22μm microporous membrane to remove insoluble impurities and obtain filtrate. The filtrate is placed in an aseptic filling machine and filled and sealed under aseptic conditions. After passing the light inspection, the post-natal plant polypeptide regulation endocrine and ovarian care compound preparation is obtained, which can be prepared into oral liquid, gel or lyophilized powder dosage form according to needs.

[0033] Example 3 A post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries, comprising the following components by mass percentage: purified water 75.0%, lactic acid bacteria fermentation lysate 3.0%, L-arabinose 2.0%, double-petaled red rose extract 1.5%, cordyceps militaris extract 1.0%, γ-aminobutyric acid 1.0%, red ginseng concentrate 1.5%, cranberry fruit powder 1.0%, turmeric extract 0.5%, vitamin C 0.5%, forest frog polypeptide powder 1.0%, carob extract 1.0%, roselle extract 0.5%, tremella polysaccharide 0.5%, and yeast β-glucan 0.5%.

[0034] Its preparation method includes the following steps: S1. Raw material pretreatment: Double-petaled red rose extract, Cordyceps militaris extract, turmeric extract, carob extract, and roselle extract were dried at 55℃ and vacuum degree -0.10MPa for 2 hours, and then ultra-finely pulverized through a 1000-mesh sieve; Forest frog polypeptide powder, Tremella fuciformis polysaccharide, and yeast β-glucan were dried at 40℃ for 1 hour; Red ginseng concentrate was pre-cooled at 8℃ for later use. S2, Liquid-solid fermentation pretreatment: Take the lactic acid bacteria fermentation lysate and mix it with 50% purified water, adjust the temperature to 37℃ and the pH value to 7.0, add 0.10% compound fermentation aid, which is a mixture of maltodextrin and fructooligosaccharide in a mass ratio of 2:1, stir and ferment at 150r / min for 8h to obtain fermentation base liquid, after fermentation is completed, pasteurize at 65℃ for 20min, and cool naturally to room temperature; S3. Gradient dissolution of active ingredients: Add the remaining 50% of purified water to the frog polypeptide powder, tremella polysaccharide, and yeast β-glucan, and stir at 50℃ and 120 r / min for 30 min to obtain a polysaccharide polypeptide solution; add L-arabinose, γ-aminobutyric acid, and vitamin C to the polysaccharide polypeptide solution, and continue stirring at 120 r / min for 15 min to obtain the basic active solution; S4. Compounding of plant components with fermentation broth: Fine powders of double-petaled red rose extract, cordyceps militaris extract, turmeric extract, carob extract, and roselle extract are added to the fermentation base broth and stirred and dispersed at 30℃ and 100r / min for 40min. Then, ultrasonic-assisted extraction is performed with an ultrasonic power of 300W and an ultrasonic time of 20min. Intermittent ultrasonication is used, with ultrasonication for 5min followed by a 2min pause. The cycle is repeated until the set ultrasonic time is reached to obtain the plant fermentation compound broth. S5. Total liquid preparation and homogenization: Mix the basic active liquid and the plant fermentation compound liquid, add the pre-cooled red ginseng concentrate and cranberry fruit powder, and stir and mix at 25℃ and 180r / min for 60min to obtain the initial liquid preparation; send the initial liquid preparation into a high-pressure homogenizer and homogenize twice at 60MPa pressure and 25℃ temperature, each time for 10min. S6. Filtration and finished product preparation: The homogenized initial solution is vacuum filtered through a 0.22μm microporous membrane to remove insoluble impurities and obtain filtrate. The filtrate is placed in an aseptic filling machine and filled and sealed under aseptic conditions. After passing the light inspection, the post-natal plant polypeptide regulation endocrine and ovarian care compound preparation is obtained, which can be prepared into oral liquid, gel or lyophilized powder dosage form according to needs.

[0035] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0036] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the uterus and ovaries, characterized in that, The product, by mass percentage, consists of the following components: purified water 60.0%–75.0%, lactic acid bacteria fermentation lysate 3.0%–6.0%, L-arabinose 2.0%–4.0%, double-petaled red rose extract 1.5%–3.5%, cordyceps militaris extract 1.0%–3.0%, γ-aminobutyric acid 1.0%–2.5%, red ginseng concentrate 1.5%–3.0%, cranberry fruit powder 1.0%–2.5%, turmeric extract 0.5%–2.0%, vitamin C 0.5%–1.5%, forest frog polypeptide powder 1.0%–3.0%, carob extract 1.0%–2.5%, roselle extract 0.5%–2.0%, tremella polysaccharide 0.5%–2.0%, and yeast β-glucan 0.5%–2.0%; the sum of the mass percentages of all components is 100%.

2. The post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries according to claim 1, characterized in that, The preferred formula, by weight percentage, is as follows: 68.5% purified water, 4.5% lactic acid bacteria fermentation lysate, 3.0% L-arabinose, 2.5% double-petaled red rose extract, 2.0% cordyceps extract, 1.8% γ-aminobutyric acid, 2.0% red ginseng concentrate, 1.8% cranberry fruit powder, 1.2% turmeric extract, 1.0% vitamin C, 2.0% forest frog polypeptide powder, 1.8% carob extract, 1.2% roselle extract, 1.2% tremella polysaccharide, and 1.5% yeast β-glucan.

3. A method for preparing a post-natal plant polypeptide compound preparation for regulating endocrine function and nourishing the ovaries as described in claim 1 or 2, characterized in that, Includes the following steps: S1. Raw material pretreatment: Double-petaled red rose extract, Cordyceps militaris extract, turmeric extract, carob extract, and roselle extract were placed in a vacuum drying oven and dried for 2-4 hours at a temperature of 45℃~55℃ and a vacuum degree of -0.08MPa~-0.10MPa. After drying, they were pulverized by an ultra-micro pulverizer and passed through an 800-1000 mesh sieve to obtain fine plant powder for later use. Forest frog polypeptide powder, Tremella fuciformis polysaccharide, and yeast β-glucan were placed in a constant temperature desiccator and dried for 1-2 hours at a temperature of 35℃~40℃ to remove surface moisture and sealed for later use. Red ginseng concentrate was pre-cooled in a refrigerated environment at 4℃~8℃ for later use. S2. Liquid-solid fermentation pretreatment: Mix 50% to 60% of the lactic acid bacteria fermentation lysate with purified water and place it in a fermenter. Adjust the temperature to 30℃ to 37℃ and the pH value to 6.0 to 7.

0. Add 0.05% to 0.10% of the total mass of the mixture as a compound fermentation aid. Stir and ferment at 100 to 150 rpm for 8 to 12 hours to obtain the fermentation base liquid. After fermentation, pasteurize at 60℃ to 65℃ for 20 to 30 minutes and cool to room temperature. S3. Gradient dissolution of active ingredients: Add the pretreated frog polypeptide powder, tremella polysaccharide, and yeast β-glucan to the remaining purified water, and stir to dissolve for 30 min to 60 min at a temperature of 40℃~50℃ and a speed of 80 r / min~120 r / min to obtain a polysaccharide polypeptide solution; add L-arabinose, γ-aminobutyric acid, and vitamin C to the solution, and continue stirring at the same speed for 15 min to 30 min to obtain the basic active solution; S4. Combining plant components with fermentation broth: Add the plant powder obtained in step S1 to the fermentation base broth in step S2, and stir and disperse for 40 min to 60 min at a temperature of 25℃~30℃ and a rotation speed of 60 r / min~100 r / min. Then, use ultrasonic-assisted extraction with an ultrasonic power of 200 W~300 W and an ultrasonic time of 20 min~30 min to obtain the plant fermentation compound broth. S5. Total liquid preparation and homogenization: Mix the basic active liquid from step S3 and the plant fermentation compound liquid from step S4, add the pre-cooled red ginseng concentrate and cranberry powder, and stir and mix for 60 min to 90 min at a temperature of 20℃~25℃ and a speed of 120 r / min~180 r / min to obtain the initial liquid preparation; send the initial liquid preparation into a high-pressure homogenizer and homogenize 2 to 3 times at a pressure of 40MPa~60MPa and a temperature of 20℃~25℃, with each homogenization lasting 10 min to 15 min; S6. Filtration and finished product preparation: The homogenized initial solution is vacuum filtered through a 0.22μm microporous membrane to remove insoluble impurities and obtain filtrate; the filtrate is placed in a sterile filling machine, filled and sealed under sterile conditions, and after passing light inspection, the post-natal plant polypeptide endocrine regulation and ovarian care compound preparation is obtained.

4. The preparation method of the post-natal plant polypeptide regulatory endocrine and ovarian care compound preparation according to claim 3, characterized in that, The compound fermentation aid mentioned in step S2 is a mixture of maltodextrin and fructooligosaccharides in a mass ratio of 1:1 to 2:

1.

5. The preparation method of the post-natal plant polypeptide endocrine-regulating and ovarian-nourishing compound preparation according to claim 3, characterized in that, In step S4, the ultrasonic-assisted extraction uses intermittent ultrasound, with 5 minutes of ultrasound followed by a 2-minute pause, and the operation is repeated until the set ultrasound time is reached.

6. The application of the compound formulation according to claim 1 or 2 in the preparation of oral liquids, gels, lyophilized powders for female endocrine regulation, uterine and ovarian care, pelvic floor muscle repair, or skin firming and moisturizing.