A microbial culture reaction device

Abstract

This invention discloses a microbial culture reaction device, belonging to the technical field of culture devices. It includes an incubator with a support plate horizontally slidably mounted inside. The support plate has evenly spaced placement slots, each containing an mounting plate. An oxygen supply component is mounted on the support plate. The oxygen supply component includes a horizontal rod slidably mounted on the mounting plate, with an oxygen supply pipe mounted on the horizontal rod. This design uses a motor-driven cam to reciprocate the support plate, achieving synchronous oscillation of the culture bottles. Simultaneously, the support plate squeezes and resets elastic air bladders, automatically extracting and directionally delivering sterile oxygen. During the oscillating culture, oxygen is continuously supplied to the culture bottles, and the airflow directly impacts the culture medium, actively agitating the microbial mixture and oxygen, significantly improving gas-liquid contact efficiency. This solves the problems of insufficient oxygen supply and uneven mixing in traditional static culture, significantly improving the microbial culture effect and proliferation rate.

Description

Technical Field

[0001] This invention relates to the field of culture apparatus technology, and more specifically, to a microbial culture reaction apparatus. Background Technology

[0002] Microbial culture reaction devices are core equipment in fields such as bio-fermentation, biomedicine, environmental monitoring, and food processing. They are mainly used for the inoculation, cultivation, amplification, and preparation of metabolites of microorganisms. By controlling conditions such as temperature, pH, dissolved oxygen, stirring, and aeration, a stable and suitable environment is provided for microbial growth.

[0003] In existing microbial culture devices, culture flasks are typically placed on a shelf inside an incubator during the microbial culture process. Most microorganisms are aerobic and require aerobic conditions to carry out their normal life activities to meet their respiratory needs. Because the culture flasks remain stationary and have narrow necks, the microorganisms cannot fully access air, hindering their growth and reproduction, and also impeding the thorough mixing of the culture medium within the flask, thus affecting microbial growth.

[0004] To address the aforementioned issues, some solutions have been proposed in existing technologies. For example, Chinese invention patent CN119752593B discloses a culture device for testing microbial bacteria. This device, through a shaking mechanism, allows the jet nozzle to intermittently deliver sterile air to culture flasks placed on a plate inside the incubator. Simultaneously, the back-and-forth movement of the plate causes the culture flasks to shake, allowing the microorganisms to have more thorough contact with the air. While shaking can improve the mixing effect of oxygen and microorganisms to some extent, in practical use, existing technologies only introduce oxygen into the reaction device and cannot directly spray oxygen into the culture flasks. After the oxygen diffuses within the reaction device, its concentration gradually decreases. Even with shaking, the mixing effect of microorganisms and oxygen remains limited, thus affecting the cultivation effect of the microorganisms. Summary of the Invention

[0005] To address the problems existing in the prior art, the present invention aims to provide a microbial culture reaction device that can improve the microbial culture effect.

[0006] To solve the above problems, the present invention adopts the following technical solution.

[0007] A microbial culture reaction device includes an incubator, a support plate that is horizontally slidably installed inside the incubator, placement slots that are evenly distributed on the support plate, an installation plate that is installed in the placement slots, and an oxygen supply component that is provided on the support plate. The oxygen supply assembly includes a horizontal rod slidably mounted on a mounting plate, an oxygen supply tube mounted on the horizontal rod, a mounting block fixedly mounted on the incubator, a motor fixedly mounted on the mounting block, an oil chamber opened on the mounting block, a cam rotatably mounted in the oil chamber and fixedly connected to the output end of the motor, a push rod cooperating with the cam fixedly mounted on the support plate, an elastic airbag jointly mounted between the support plate and the incubator, an air storage box fixedly mounted on the inner bottom wall of the incubator, an exhaust valve fixedly mounted on the air storage box, a first air tube communicating with the elastic airbag fixedly mounted on the output end of the exhaust valve, a first one-way valve fixedly mounted on the elastic airbag, and a communication component communicating with the oxygen supply tube on the first one-way valve.

[0008] Furthermore, the placement groove is provided with a base plate, a push plate is horizontally slidably mounted on the base plate, an elastic pad is installed between the push plate and the base plate, a magnet is fixedly mounted on the push plate, a conductive slip ring electrically connected to an external control power supply is fixedly mounted on the base plate, and an electromagnet electrically connected to the conductive slip ring is fixedly mounted on the base plate.

[0009] Furthermore, the mounting plate has a vertical groove, and the horizontal rod is vertically slidably installed in the vertical groove. A linkage block is slidably installed on the mounting plate. A first spring is installed between the linkage block and the mounting plate. A connecting rope is installed between the linkage block and the horizontal rod. A linkage spring is installed between the horizontal rod and the mounting plate. A guide wheel that cooperates with the connecting rope is rotatably installed on the mounting plate.

[0010] Furthermore, a gear is fixedly installed on the base plate, a rack that meshes with the gear is fixedly installed inside the incubator, and the base plate is rotatably installed in the placement groove.

[0011] Furthermore, the connecting component includes a second air pipe fixedly installed on the output end of the first one-way valve, and an annular air groove communicating with the second air pipe is provided on the base plate, and the oxygen supply pipe is connected to the annular air groove.

[0012] Furthermore, the push plate has a cavity, the cavity has an air hole that communicates with the outside, the elastic pad has a linkage cavity, and a fourth air tube is inserted into the linkage cavity.

[0013] Furthermore, a sealing rod is slidably mounted on the push plate, and a return spring is installed between the sealing rod and the cavity. A groove communicating with the fourth air pipe is opened on the sealing rod.

[0014] Furthermore, a buffer pad is fixedly installed on the side wall of the push plate, and a second one-way valve with its output end connected to the outside is inserted into the linkage cavity.

[0015] Furthermore, a limiting rod that cooperates with the support plate is fixedly installed inside the incubator.

[0016] Furthermore, an oil injection valve is provided on the side wall of the oil cavity, and an oil discharge valve is provided on the side wall of the oil cavity.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) This scheme uses a motor-driven cam to drive the support plate to slide back and forth, so as to realize the synchronous swing of the culture bottle. At the same time, the support plate squeezes and resets the elastic air bag to complete the automatic extraction and directional delivery of sterile oxygen. While swinging and culturing, oxygen is continuously supplied to the culture bottle. The airflow directly impacts the culture medium, forming an active agitation between the microbial mixture and oxygen, which greatly improves the gas-liquid contact efficiency, solves the problems of insufficient oxygen supply and uneven mixing in traditional static culture, and significantly improves the microbial culture effect and proliferation rate. Moreover, the weight of the culture bottle and shaking can easily cause it to dislodge. The mechanical action of the push plate contacting the culture bottle spontaneously extracts the vacuum of the linkage chamber and performs active negative pressure adsorption on the culture bottle, ensuring absolute stability under violent shaking.

[0018] (2) This scheme uses the connection of the connecting rope and the horizontal bar to push the linkage block during the clamping process of the culture bottle. The connecting rope and the guide wheel drive the horizontal bar and the oxygen supply tube to automatically move down and insert into the culture bottle, ensuring that oxygen directly acts on the deep layer of the culture medium, further enhancing the airflow stirring effect, and making the microorganisms and oxygen mix more fully.

[0019] (3) This scheme uses the gear and rack to rotate the culture bottle when the support plate moves. This causes the culture medium to form a continuously renewed thin liquid film in the bottle, which greatly increases the effective contact area between the culture medium and oxygen, improves the dissolved oxygen efficiency, and, in conjunction with the high-frequency lifting and unloading of the oxygen supply tube, realizes the dual three-dimensional forced oxygen supply of dynamic liquid film and deep air bubbles. This breaks the dissolved oxygen bottleneck of traditional static or single shaker culture and further improves the microbial culture effect. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a cross-sectional view of the incubator and support plate of the present invention; Figure 3 For the present invention Figure 2 Enlarged view of point A in the middle; Figure 4 This is a diagram showing the combination of the gear, rack, and support plate of the present invention. Figure 5 This is a cross-sectional view of the base plate and push plate of the present invention; Figure 6 For the present invention Figure 5 Enlarged view at point B in the middle; Figure 7 This is a cross-sectional view of the mounting plate of the present invention.

[0021] Explanation of the labels in the diagram: 101. Incubator; 102. Support plate; 2. Oxygen supply assembly; 201. Mounting plate; 202. Horizontal bar; 203. Oxygen supply pipe; 204. Mounting block; 205. Motor; 206. Oil chamber; 207. Cam; 208. Push rod; 209. Elastic airbag; 210. Air tank; 211. Exhaust valve; 212. First check valve; 213. First air pipe; 301. Base plate; 302. Push plate; 303. Elastic pad; 304. Magnetic block; 305. Conductive slip ring; 306. Electromagnet; 401. Linkage block; 402. First spring; 403. Connecting rope; 404. Linkage spring; 405. Guide wheel; 501. Gear; 502. Rack; 6. Connecting component; 601. Second air pipe; 602. Annular air groove; 604. Cavity; 605. Air hole; 606. Linkage chamber; 607. Fourth air pipe; 608. Sealing rod; 609. Return spring; 610. Groove; 701. Buffer pad; 702. Second check valve; 703. Limiting rod; 704. Oil injection valve; 705. Oil discharge valve. Detailed Implementation

[0022] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0023] Please see Figures 1 to 7 A microbial culture reaction device includes an incubator 101, characterized in that: a support plate 102 is horizontally slidably installed inside the incubator 101, and a placement groove is evenly opened on the support plate 102. An installation plate 201 is provided in the placement groove, and an oxygen supply component 2 is provided on the support plate 102. The oxygen supply assembly 2 includes a horizontal rod 202 slidably mounted on a mounting plate 201, an oxygen supply pipe 203 mounted on the horizontal rod 202, a mounting block 204 fixedly mounted on the incubator 101, a motor 205 fixedly mounted on the mounting block 204, an oil cavity 206 formed in the mounting block 204, a cam 207 rotatably mounted in the oil cavity 206 and fixedly connected to the output end of the motor 205, and a push rod 208 cooperating with the cam 207 fixedly mounted on the support plate 102. An elastic airbag 209 is installed between the support plate 102 and the incubator 101. An air storage box 210 is fixedly installed on the inner bottom wall of the incubator 101. An exhaust valve 211 is fixedly installed on the air storage box 210. A first air pipe 213 communicating with the elastic airbag 209 is fixedly installed on the output end of the exhaust valve 211. A first one-way valve 212 is fixedly installed on the elastic airbag 209. A connecting component 6 communicating with the oxygen supply pipe 203 is provided on the first one-way valve 212.

[0024] In use, first open the door of the incubator 101, then place the culture flask in the placement slot. After placement, close the door and then start the motor 205. During the operation of the motor 205, the motor drives the cam 207 to rotate. As the cam 207 rotates, it gradually contacts the push rod 208, which in turn drives the support plate 102 to compress the elastic airbag 209. When the push rod 208 disengages from the cam 207, the elastic airbag 209 extends and causes the support plate 102 to return to its original position. Simultaneously, during the extension of the elastic airbag 209, it draws sterile oxygen from the incubator 101 through the exhaust valve 211. Then, as the support plate 102 compresses the elastic airbag 209, the airflow inside the elastic airbag 209 flows through the first one-way valve 212 and the connecting component 6 to the oxygen supply pipe 203, and then through the oxygen supply pipe 203 to the culture flask. In this way, oxygen can be injected into the culture flask while it is being swung. Moreover, when oxygen flows to the culture flask through the oxygen supply tube 203, the airflow impacts the solution inside the culture flask, thereby agitating the microbial mixture and oxygen, ensuring that the microorganisms and oxygen are fully mixed, which improves the microbial culture effect.

[0025] like Figures 4 to 7 As shown, a base plate 301 is provided in the placement groove, and a push plate 302 is horizontally slidably installed on the base plate 301. An elastic pad 303 is installed between the push plate 302 and the base plate 301. A magnet block 304 is fixedly installed on the push plate 302. A conductive slip ring 305 electrically connected to an external control power supply is fixedly installed on the base plate 301, and an electromagnet 306 electrically connected to the conductive slip ring 305 is fixedly installed on the base plate 301.

[0026] The mounting plate 201 has a vertical groove, and the horizontal rod 202 is vertically slidably installed in the vertical groove. A linkage block 401 is slidably installed on the mounting plate 201. A first spring 402 is installed between the linkage block 401 and the mounting plate 201. A connecting rope 403 is installed between the linkage block 401 and the horizontal rod 202. A linkage spring 404 is installed between the horizontal rod 202 and the mounting plate 201. A guide wheel 405 that cooperates with the connecting rope 403 is rotatably installed on the mounting plate 201.

[0027] A gear 501 is fixedly installed on the base plate 301, and a rack 502 that meshes with the gear 501 is fixedly installed inside the incubator 101. The base plate 301 is rotatably installed in the placement groove.

[0028] By adopting the above technical solution, when the motor 205 starts, since the conductive slip ring 305 is connected in series with the motor 205 to the external control power supply, the conductive slip ring 305 is energized when the motor 205 starts, and then the electromagnet 306 is energized and generates a magnetic field. Because the magnet 304 and the electromagnet 306 repel each other, under the action of the magnetic field, the magnet 304 drives the push plate 302 to move and stretches the elastic pad 303. During the movement of the push plate 302, it gradually comes into contact with the culture bottle and pushes the culture bottle into contact with the mounting plate 201. Then, under the combined action of the mounting plate 201 and the push plate 302, the culture bottle is clamped, thereby preventing the culture bottle from tilting or being damaged during the swinging process driven by the support plate 102, further improving the microbial culture effect.

[0029] As the pusher plate 302 moves the culture bottle toward the mounting plate 201, the culture bottle gradually comes into contact with the linkage block 401, causing the linkage block 401 to compress the first spring 402. During this compression, the linkage block 401 gradually stretches the connecting rope 403. Then, under the action of the guide wheel 405, the connecting rope 403 drives the horizontal rod 202 downwards, at which point the linkage spring 404 is stretched and tends to return to its original position. As the horizontal rod 202 moves downwards, it drives the oxygen supply tube 203 downwards, inserting it into the culture bottle. This ensures sufficient oxygen flow into the culture bottle and enhances the impact of the airflow on the microbial mixture solution inside the culture bottle, thereby improving the mixing effect of microorganisms and oxygen and further enhancing the microbial cultivation effect.

[0030] During the movement of the culture bottle driven by the support plate 102 through the base plate 301, the rack 502 drives the base plate 301 to rotate through the gear 501. During the rotation of the base plate 301, the base plate 301 drives the culture bottle to rotate through the push plate 302 and the mounting plate 201. During the rotation of the culture bottle, the microbial mixed solution can form a continuously renewed liquid film inside the bottle, which greatly increases the contact area with oxygen and further improves the microbial culture effect.

[0031] like Figure 5 , Figure 6 As shown, the connecting component 6 includes a second air pipe 601 fixedly installed on the output end of the first one-way valve 212, and an annular air groove 602 communicating with the second air pipe 601 is provided on the base plate 301. The oxygen supply pipe 203 is connected to the annular air groove 602.

[0032] The push plate 302 has a cavity 604, the cavity 604 has an air hole 605 communicating with the outside, the elastic pad 303 has a linkage cavity 606, and a fourth air tube 607 is inserted into the linkage cavity 606.

[0033] A sealing rod 608 is slidably mounted on the push plate 302. A return spring 609 is installed between the sealing rod 608 and the cavity 604. A groove 610 communicating with the fourth air pipe 607 is opened on the sealing rod 608.

[0034] By adopting the above technical solution, during the process of the push plate 302 stretching the elastic pad 303, the pressure inside the linkage cavity 606 decreases, and during the movement of the push plate 302, the sealing rod 608 gradually comes into contact with the culture bottle. Then, the culture bottle drives the sealing rod 608 to move horizontally and compress the return spring 609. When the side wall of the push plate 302 is in contact with the culture bottle, the sealing rod 608 drives the groove 610 to connect with the fourth gas tube 607. At this time, the linkage cavity 606 draws air from the cavity 604 through the fourth gas tube 607 and the groove 610, thereby reducing the pressure inside the cavity 604. Since the side wall of the push plate 302 is in contact with the side wall of the culture bottle at this time, the culture bottle can block the pore 605. Then, under the action of pressure, the push plate 302 is adsorbed together with the culture bottle through the pore 605, which strengthens the fixation effect of the culture bottle. This prevents relative rotation between the culture bottle and the push plate 302 when the push plate 302 and the mounting plate 201 drive the culture bottle to rotate, and further improves the microbial culture effect.

[0035] like Figure 2 , Figure 4 , Figure 5 , Figure 6 As shown, a buffer pad 701 is fixedly installed on the side wall of the push plate 302, and a second one-way valve 702 with its output end connected to the outside is inserted into the linkage cavity 606.

[0036] The incubator 101 is fixedly installed with a limiting rod 703 that cooperates with the support plate 102.

[0037] An oil injection valve 704 is provided on the side wall of the oil chamber 206, and an oil discharge valve 705 is provided on the side wall of the oil chamber 206.

[0038] By adopting the above technical solution, when the push plate 302 and the mounting plate 201 clamp the culture flask, the buffer pad 701 deforms and fits tightly against the culture flask, which can protect the culture flask and improve the clamping effect. In addition, it can also improve the sealing between the culture flask and the vent 605, ensuring that the push plate 302 and the culture flask can adhere together.

[0039] By setting the limiting rod 703, the support plate 102 can be vertically limited, ensuring that the support plate 102 can only slide in the horizontal direction and preventing the support plate 102 from getting stuck. During the movement of the push rod 208 driven by the cam 207, lubricating oil can be injected into the oil chamber 206 through the oil injection valve 704 to reduce the friction between the cam 207 and the push rod 208. After the cam 207 has been working for a long time, waste oil can be discharged through the oil drain valve 705.

[0040] Instructions for use: First, open the door of the incubator 101 and place the culture flask containing the microorganisms to be cultured stably in the placement slot of the support plate 102. After placement, close the door of the incubator 101 and turn on the main power supply of the device. At the same time as the motor 205 starts, the conductive slip ring 305 is simultaneously energized, causing the electromagnet 306 to become energized and generate magnetism. The magnet block 304 on the push plate 302 pushes the push plate 302 to move towards the culture flask. With the cooperation of the push plate 302 and the mounting plate 201, the culture flask is automatically clamped and fixed.

[0041] Then, as the pusher plate 302 pushes the culture bottle closer to the mounting plate 201, the culture bottle touches and squeezes the linkage block 401. As the linkage block 401 moves, it drives the horizontal rod 202 and the oxygen supply tube 203 to move downward through the connecting rope 403, so that the oxygen supply tube 203 is automatically inserted into the culture bottle.

[0042] At the same time, the motor 205 drives the cam 207 to rotate continuously. The cam 207 periodically pushes the push rod 208 and the support plate 102 to move horizontally, repeatedly squeezing and releasing the elastic airbag 209. When the elastic airbag 209 extends, the elastic airbag 209 draws in sterile oxygen from the air tank 210 through the exhaust valve 211.

[0043] In addition, the support plate 102 slides horizontally back and forth under the action of the cam 207 and the elastic air bag 209, causing the culture bottle to swing as a whole, so that the culture medium and oxygen are initially mixed. When the support plate 102 moves, the gear 501 on the bottom plate 301 meshes with the rack 502 in the box, causing the bottom plate 301, the push plate 302 and the culture bottle to rotate, so that the culture medium forms a liquid film, greatly increasing the oxygen contact area. At the same time, the oxygen airflow sprayed from the oxygen supply pipe 203 impacts the culture medium, creating disturbance.

[0044] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and its improved concepts, should be covered within the scope of protection of the present invention.

Claims

1. A microbial culture reaction apparatus, comprising an incubator (101), characterized in that: A support plate (102) is horizontally slidably installed inside the incubator (101). Placement slots are evenly provided on the support plate (102), and an installation plate (201) is provided in the placement slots. An oxygen supply component (2) is provided on the support plate (102). The oxygen supply assembly (2) includes a horizontal rod (202) slidably mounted on a mounting plate (201), an oxygen supply pipe (203) on the horizontal rod (202), a mounting block (204) fixedly mounted on the incubator (101), a motor (205) fixedly mounted on the mounting block (204), an oil cavity (206) opened on the mounting block (204), a cam (207) fixedly connected to the output end of the motor (205) rotatably mounted in the oil cavity (206), and a push rod (207) cooperating with the cam (207) fixedly mounted on the support plate (102). 8) An elastic airbag (209) is installed between the support plate (102) and the incubator (101). An air storage box (210) is fixedly installed on the inner bottom wall of the incubator (101). An exhaust valve (211) is fixedly installed on the air storage box (210). A first air pipe (213) communicating with the elastic airbag (209) is fixedly installed on the output end of the exhaust valve (211). A first one-way valve (212) is fixedly installed on the elastic airbag (209). A connecting component (6) communicating with the oxygen supply pipe (203) is provided on the first one-way valve (212).

2. The microbial culture reaction apparatus according to claim 1, characterized in that: The placement slot is provided with a base plate (301), and a push plate (302) is horizontally slidably installed on the base plate (301). An elastic pad (303) is installed between the push plate (302) and the base plate (301). A magnet block (304) is fixedly installed on the push plate (302). A conductive slip ring (305) electrically connected to an external control power supply is fixedly installed on the base plate (301). An electromagnet (306) electrically connected to the conductive slip ring (305) is fixedly installed on the base plate (301).

3. The microbial culture reaction apparatus according to claim 2, characterized in that: The mounting plate (201) has a vertical groove, and the horizontal rod (202) is vertically slidably installed in the vertical groove. A linkage block (401) is slidably installed on the mounting plate (201). A first spring (402) is installed between the linkage block (401) and the mounting plate (201). A connecting rope (403) is installed between the linkage block (401) and the horizontal rod (202). A linkage spring (404) is installed between the horizontal rod (202) and the mounting plate (201). A guide wheel (405) that cooperates with the connecting rope (403) is rotatably installed on the mounting plate (201).

4. The microbial culture reaction apparatus according to claim 2, characterized in that: A gear (501) is fixedly installed on the base plate (301), and a rack (502) that meshes with the gear (501) is fixedly installed inside the incubator (101), and the base plate (301) is rotatably installed in the placement groove.

5. The microbial culture reaction apparatus according to claim 4, characterized in that: The connecting component (6) includes a second air pipe (601) fixedly installed on the output end of the first one-way valve (212), and an annular air groove (602) communicating with the second air pipe (601) is provided on the base plate (301), and the oxygen supply pipe (203) is connected to the annular air groove (602).

6. The microbial culture reaction apparatus according to claim 5, characterized in that: The push plate (302) has a cavity (604), the cavity (604) has an air hole (605) communicating with the outside, the elastic pad (303) has a linkage cavity (606), and a fourth air tube (607) is inserted into the linkage cavity (606).

7. The microbial culture reaction apparatus according to claim 6, characterized in that: A sealing rod (608) is slidably installed on the push plate (302). A return spring (609) is installed between the sealing rod (608) and the cavity (604). A groove (610) communicating with the fourth air pipe (607) is opened on the sealing rod (608).

8. A microbial culture reaction apparatus according to claim 6, characterized in that: A buffer pad (701) is fixedly installed on the side wall of the push plate (302), and a second one-way valve (702) with its output end connected to the outside is inserted into the linkage cavity (606).

9. The microbial culture reaction apparatus according to claim 1, characterized in that: The incubator (101) is fixedly installed with a limiting rod (703) that cooperates with the support plate (102).

10. A microbial culture reaction apparatus according to claim 1, characterized in that: An oil injection valve (704) is provided on the side wall of the oil chamber (206), and an oil discharge valve (705) is provided on the side wall of the oil chamber (206).

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