A microbial detection device for deep-processed chestnut products

Abstract

This invention discloses a microbial detection device for deep-processed chestnut products, belonging to the technical field of microbial detection devices for deep-processed products. It includes a frame, a crushing mechanism at the top of the frame, a mixing mechanism below the crushing mechanism, a three-way valve below the mixing mechanism, an overflow pipe and a clamp valve at the other two ports of the three-way valve, a sampling needle below the clamp valve, and a double-layer rotating sampling mechanism below the sampling needle, with a sample tube mounted on the double-layer rotating sampling mechanism. This invention utilizes tangential jetting at the throat of the Venturi tube to create a high-speed vortex, ensuring that starch particles remain suspended and guaranteeing sample representativeness. The integrated crushing and filtration, continuous homogenization, and the three-way overflow clamp valve work together to achieve precise matching of continuous flow and intermittent sampling for dispensing. The inner rotating drum is self-cleaning, the Venturi tube has no moving parts, and the clamp valve has a flexible hose contact design, combined with circulating cleaning, reducing cleaning difficulty and preventing cross-contamination.

Description

Technical Field

[0001] This invention relates to the field of microbial detection devices for deep-processed products, and more particularly to a microbial detection device for deep-processed chestnut products. Background Technology

[0002] Chestnut processed products (such as chestnut puree, canned chestnuts in syrup, and chestnut flour) are traditional agricultural byproducts in my country, and their microbiological testing is a crucial step in ensuring food safety. Because chestnuts are rich in starch, processed products often exhibit high viscosity, high starch content, and a mixture of solids and liquids (e.g., canned chestnuts in syrup require simultaneous testing of both solids and broth, while chestnut puree is a semi-solid paste). This makes sample preparation before microbiological testing extremely difficult.

[0003] Currently, the main methods used for pretreatment of chestnut deep-processed products for microbial detection are as follows: Manual chopping and grinding method: The operator cuts the chestnut sample into small pieces with scissors, adds a diluent, grinds it manually with a glass rod, and then filters it through sterile gauze. This method is not only inefficient, but also exposes the sample to air for a long time, which can easily cause secondary contamination and affect the accuracy of the test results.

[0004] Conventional homogenizer processing method: Samples are processed using a tapping homogenizer or a blade homogenizer. However, for high-starch samples such as chestnuts, the following problems exist: Sedimentation problem: Starch particles settle rapidly after homogenization, resulting in an inability to obtain a uniform suspension during sampling, leading to large deviations in test results; Clogging problem: High-viscosity slurry easily clogs the blade and tubing, making cleaning difficult and causing cross-contamination. Summary of the Invention

[0005] The purpose of this invention is to provide a microbial detection device for deep-processed chestnut products, which solves the problems of rapid sedimentation of chestnut starch granules after homogenization, the inability of existing homogenization devices to maintain the uniform suspension of samples before sampling, resulting in the samples taken not representing the true contamination level of the entire batch of products, large deviations in test results, and the easy adhesion of high starch samples to the inner walls of pipelines and equipment, which is difficult to completely remove by ordinary cleaning methods, causing cross-contamination between batches.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: This invention discloses a microbial detection device for deep-processed chestnut products, comprising a frame, a crushing mechanism at the top of the frame, a mixing mechanism below the crushing mechanism, a tee below the mixing mechanism, an overflow pipe and a clamp valve at the other two ports of the tee, a sampling needle below the clamp valve, a double-layer rotary sampling mechanism below the sampling needle, and a sample tube on the double-layer rotary sampling mechanism.

[0007] Furthermore, the crushing mechanism includes a crushing box disposed on the top of the frame, an openable door disposed on the right side of the crushing box, an inner rotating drum disposed inside the crushing box and a drive assembly for driving the inner rotating drum to rotate, a plurality of filter holes disposed on the inner rotating drum, a second drive motor disposed on the outside of the crushing box, the output shaft of the second drive motor being connected to a rotating shaft located inside the inner rotating drum, a crushing blade disposed on the rotating shaft, and a discharge hopper disposed at the bottom of the crushing box.

[0008] Furthermore, the box door is equipped with a liquid inlet pipe.

[0009] Furthermore, the drive assembly includes a gear ring disposed on the outside of the inner rotating drum, and a first drive motor is disposed on the outside of the crushing box. The output shaft of the first drive motor extends into the crushing box and is connected to the gear, which meshes with the gear ring.

[0010] Furthermore, the mixing mechanism includes a mixing tube disposed below the discharge hopper, the mixing tube having a fluid inlet and a fluid outlet, and a fluid channel connecting the fluid inlet and the fluid outlet, the fluid channel including at least one acceleration section, the cross-sectional area of ​​the acceleration section being smaller than the cross-sectional area of ​​the fluid inlet and / or the fluid outlet; at least one high-pressure sterile gas injection pipe is provided on the wall of the mixing tube.

[0011] Furthermore, the mixing tube is configured as a Venturi tube, and at least one high-pressure sterile gas injection tube is provided on the throat wall of the Venturi tube.

[0012] Furthermore, the high-pressure sterile gas injection pipe is arranged along the tangential direction of the venturi tube throat wall.

[0013] Furthermore, the double-layer rotary sampling mechanism includes a lower support tray and an upper positioning plate. The surface of the lower support tray is provided with a groove that matches the bottom of the sample tube. The upper positioning plate is provided with a positioning hole that matches the outer diameter of the sample tube. The distance between the lower support tray and the upper positioning plate is adjustable to match sample tubes of different heights. A rotary drive assembly for driving the lower support tray and the upper positioning plate to rotate is provided below the lower support tray.

[0014] Furthermore, the rotary drive assembly includes a rotary seat disposed on the top of the support frame, a lower support tray and an upper positioning plate disposed above the rotary seat, and a first drive structure for driving the rotary seat to rotate disposed on the outer side of the rotary seat. The drive structure includes a first synchronous pulley disposed on the outside of the rotating seat, a third drive motor disposed on the support frame, and a second synchronous pulley disposed on the output shaft of the third drive motor. The first synchronous pulley and the second synchronous pulley are rotatably connected by a synchronous belt.

[0015] Furthermore, the top surface of the rotating seat is provided with several fixed tubes, a lifting rod is provided above the fixed tubes, an upper positioning plate is provided on the top surface of the lifting rod, a lower support plate is provided on the outside of the fixed tubes, a screw is provided at the center of the rotating seat, a second driving structure for driving the screw to rotate is provided on the support frame, a lifting tube is provided at the center of the bottom surface of the upper positioning plate, the lifting tube is provided with a thread matching the screw, a fixed sleeve is provided at the center of the several fixed tubes, and the lifting tube moves along the fixed sleeve; The second drive structure includes a second bevel gear disposed at the bottom of the screw, a fourth drive motor disposed on the support frame, and a first bevel gear disposed on the output shaft of the fourth drive motor, the first bevel gear meshing with the second bevel gear.

[0016] Compared with the prior art, the beneficial technical effects of the present invention are as follows: This invention effectively prevents starch sedimentation and ensures the representativeness of the samples through a combination of a Venturi tube and tangential jet injection. The narrowed throat section of the Venturi tube causes a sharp increase in the flow velocity of the solid-liquid mixture, generating strong turbulence. The tangentially positioned high-pressure sterile gas injection tube forms a high-speed rotating airflow at the throat, driving the liquid to swirl. This causes starch particles to be thrown against the tube wall by centrifugal force and immediately dispersed by the airflow, remaining in a suspended state.

[0017] The present invention features a crushing mechanism that automatically crushes and filters samples; a mixing mechanism that continuously homogenizes online without requiring batch-based shutdowns; and a sampling module that automatically delivers multiple sample tubes sequentially to the sample dispensing needle via an adjustable double-layer rotating sampling disc for continuous automatic dispensing. The combined design of the tee, overflow pipe, and clamp valve allows for perfect docking of the continuously flowing venturi tube with the intermittent rotating sampling disc, enabling precise sample dispensing without complex timing control.

[0018] This invention reduces cleaning difficulty and prevents cross-contamination through structural design and selectable cleaning modes. The filter holes of the inner rotating cylinder have a self-cleaning function during rotation, which can prevent starch gelatinization and clogging; the Venturi tube has no moving parts inside, and the smooth inner wall does not easily adhere to materials; the design of the pinch valve ensures that the liquid only contacts the hose and not the metal parts of the valve, avoiding material residue at the valve core; cleaning fluid can be injected into the system through the inlet pipe to start the crushing and homogenizing mechanism for circulation cleaning, and sterile gas can be introduced for purging and drying. Attached Figure Description

[0019] The present invention will be further described below with reference to the accompanying drawings.

[0020] Figure 1 This is a front view of the microbial detection device for deep-processed chestnut products of the present invention; Figure 2 This is a cross-sectional view of the microbial detection device for deep-processed chestnut products of the present invention; Figure 3 This is a schematic diagram of the structure of the driving component of the present invention; Figure 4 This is a schematic diagram of the structure of the second drive motor, rotating shaft, and crushing blade of the present invention; Figure 5 This is a schematic diagram of the structure of the crusher blade of the present invention; Figure 6 This is a front view of the venturi tube of the present invention; Figure 7 This is a cross-sectional view of the high-pressure sterile gas injection pipe of the present invention; Figure 8 This is a front view of the double-layer rotating sampling mechanism of the present invention; Figure 9 This is a cross-sectional view of the double-layer rotating sampling mechanism of the present invention.

[0021] Explanation of reference numerals in the attached diagram: 1. Frame; 2. Crushing chamber; 3. Chamber door; 4. Inner rotating drum; 5. Gear ring; 6. First drive motor; 7. Gear; 8. Second drive motor; 9. Rotating shaft; 10. Crushing blade; 11. Liquid inlet pipe; 12. Discharge hopper; 13. Venturi tube; 14. High-pressure sterile gas injection pipe; 15. T-joint; 16. Overflow pipe; 17. Pinch valve; 18. Sampling needle; 19. Sample tube; 20. Lower support tray ; 2001, Groove; 21, Upper positioning plate; 2101, Positioning hole; 22, Support frame; 23, Rotating seat; 24, Fixing tube; 25, Lifting rod; 26, Fixing sleeve; 27, Screw; 28, Lifting tube; 29, Third drive motor; 30, First synchronous pulley; 31, Second synchronous pulley; 32, Synchronous belt; 33, Fourth drive motor; 34, First bevel gear; 35, Second bevel gear; 36, Protective box. Detailed Implementation

[0022] like Figure 1-9 As shown, a microbial detection device for deep-processed chestnut products includes a frame 1. A crushing mechanism is provided at the top of the frame 1, and a mixing mechanism is provided below the crushing mechanism. A tee is provided below the mixing mechanism. An overflow pipe 16 and a clamp valve 17 are respectively provided at the other two ports of the tee 15. A sampling needle 18 is provided below the clamp valve 17. A double-layer rotary sampling mechanism is provided below the sampling needle 18. A sample tube 19 is provided on the double-layer rotary sampling mechanism.

[0023] like Figure 3-5 As shown, the crushing mechanism includes a crushing box 2 installed on the top of the frame 1. An openable door 3 is installed on the right side of the crushing box 2. An inner rotating cylinder 4 and a drive assembly for driving the inner rotating cylinder 4 to rotate are provided inside the crushing box 2. Several filter holes are opened on the inner rotating cylinder 4. When the material is crushed to a size smaller than the hole diameter, the mixture flows out automatically. This is equivalent to merging the crushing and filtering steps into the same sealed cavity, reducing intermediate steps. A second drive motor 8 is installed on the outside of the crushing box 2. The output shaft of the second drive motor 8 is connected to a rotating shaft 9 located inside the inner rotating cylinder 4. A crushing blade 10 is installed on the rotating shaft 9. A discharge hopper 12 is connected to the bottom of the crushing box 2.

[0024] The box door 3 is connected to a liquid inlet pipe 11, through which solvent can be added into the inner rotating cylinder 4.

[0025] The drive assembly includes a gear ring 5 disposed on the outside of the inner rotating drum 4, and a first drive motor 6 is installed on the outside of the crushing box 2. The first drive motor 6 is installed on the outside of the crushing box 2 to facilitate heat dissipation. The output shaft of the first drive motor 6 extends into the crushing box 2 and is connected to a gear 7. The gear 7 meshes with the gear ring 5.

[0026] The second drive motor 8 drives the rotating shaft 9 and the crushing blade 10 to rotate, and the drive assembly drives the inner rotating drum 4 to rotate. The inner rotating drum 4 and the crushing blade 10 can be controlled independently. If the inner rotating drum 4 and the crushing blade 10 rotate in the same direction and at similar speeds, the relative speed is small, which is suitable for gentle stirring and protecting fragile microorganisms. If the inner rotating drum 4 and the crushing blade 10 rotate in opposite directions, the relative speed is extremely high, forming strong turbulence and shearing force, which is suitable for quickly crushing hard chestnut blocks. At the same time, since the inner rotating drum 4 itself is rotating, it generates centrifugal force. This centrifugal force not only helps the liquid flow out, but also throws away large particles of material stuck to the holes, playing a self-cleaning and anti-clogging role.

[0027] In use, chestnuts and solvent are directly added into the rotating inner drum 4, and the central crushing blade 10 performs shearing and crushing. Qualified small particles are thrown out / flow out through the holes in the drum wall under the action of centrifugal force and gravity, while large particles are trapped in the inner drum 4 for further crushing.

[0028] The mixing mechanism includes a mixing tube disposed below the discharge hopper 12. The mixing tube has a fluid inlet and a fluid outlet, and a fluid channel connecting the fluid inlet and the fluid outlet. The fluid channel includes at least one acceleration section, the cross-sectional area of ​​which is smaller than the cross-sectional area of ​​the fluid inlet and / or the fluid outlet. At least one high-pressure sterile gas injection pipe 14 is provided on the wall of the mixing tube.

[0029] like Figure 6-7 As shown, the mixing tube is a Venturi tube 13, and at least one high-pressure sterile gas injection pipe 14 is provided on the throat wall of the Venturi tube 13. The high-pressure sterile gas injection pipe 14 is arranged tangentially to the throat wall of the Venturi tube 13. After the gas is injected tangentially, a high-speed rotating airflow is immediately formed in the throat, which drives the liquid to rotate together and generates a strong vortex. This vortex causes the starch particles to be subjected to centrifugal force, thrown against the tube wall and immediately dispersed by the airflow, making it impossible for them to adhere or settle. In addition, the high-pressure sterile gas injection pipe 14 on the throat wall serves as the first-stage jet assembly. On the wall of the diffuser section adjacent to the throat, 2-4 tangential high-pressure sterile gas injection pipes are arranged, with their directions being the same as or opposite to those of the high-pressure sterile gas injection pipe 14 on the throat wall, serving as the second-stage jet assembly. These are mainly used to supplement energy and maintain the swirling flow. The first-stage jet assembly breaks up the gas in the throat, forming a gas-liquid mixed turbulent flow. The second-stage jet assembly continues to jet gas in the diffuser section to prevent boundary layer separation and starch sedimentation caused by the expansion of the cross-section and the slowing of the flow velocity. The two-stage jet assembly work together to keep the mixture turbulent in the diffuser section. Alternatively, multiple high-pressure sterile gas injection pipes 14 can be arranged along a spiral line on the throat wall. The angle of each high-pressure sterile gas injection pipe 14 is slightly different, but the overall direction is tangential, forming a spiral airflow distribution. The gas is ejected from the multiple spirally distributed high-pressure sterile gas injection pipes 14, forming a three-dimensional spiral flow field in the throat, so that the liquid is subjected to strong mixing in both the radial and axial directions, resulting in extremely high mixing uniformity.

[0030] like Figure 8-9 As shown, the double-layer rotary sampling mechanism includes a lower support tray 20 and an upper positioning plate 21. The surface of the lower support tray 20 is provided with a groove 2001 that matches the bottom of the sample tube 19. The upper positioning plate 21 is provided with a positioning hole 2101 that matches the outer diameter of the sample tube 19. The edge of the positioning hole 2101 can be designed with an elastic snap ring or a silicone washer for fixing sample tubes 19 of different specifications. The distance between the lower support tray 20 and the upper positioning plate 21 can be adjusted to match sample tubes 19 of different heights. A rotary drive assembly for driving the lower support tray 20 and the upper positioning plate 21 to rotate is provided below the lower support tray 20.

[0031] The rotary drive assembly includes a rotary seat 23 rotatably mounted on the top of the support frame 22 via bearings. The lower support tray 20 and the upper positioning plate 21 are arranged above the rotary seat 23. A first drive structure for driving the rotary seat 23 to rotate is arranged on the outer side of the rotary seat 23. The drive structure includes a first synchronous pulley 30 mounted on the outside of the rotating seat 23, a third drive motor 29 mounted on the support frame 22, a second synchronous pulley 31 mounted on the output shaft of the third drive motor 29, and the first synchronous pulley 30 and the second synchronous pulley 31 being rotatably connected by a synchronous belt 32.

[0032] The top surface of the rotating base 23 is connected to two fixed tubes 24, which are arranged symmetrically on the left and right. A lifting rod 25 is installed above each fixed tube 24. An upper positioning plate 21 is connected to the top surface of the lifting rod 25, and the bottom of the lifting rod 25 is inserted into the fixed tube 24. The lifting rod 25 and the fixed tube 24 are slidably connected, allowing the lifting rod 25 to rise and fall vertically. A lower support tray 20 is connected to the outside of the fixed tube 24. From a top view, the lower support tray 20 has a circular structure, and the hollow cavity facilitates the arrangement of other structural components. The rotating base 23... A screw 27 is rotatably mounted on the support frame 22. A second drive structure for driving the screw 27 to rotate is mounted on the support frame 22. A lifting tube 28 is connected to the center of the bottom surface of the upper positioning plate 21. The lifting tube 28 has a thread that matches the screw 27. The screw 27 is threadedly connected to the lifting tube 28, converting the rotational motion of the screw 27 into the lifting motion of the lifting tube 28. A fixing sleeve 26 is connected to the center of the two fixing tubes 24. The lifting tube 28 is slidably connected to the fixing sleeve 26, and the lifting tube 28 moves up and down along the fixing sleeve 26. The second drive structure includes a second bevel gear 35 mounted on the bottom of the screw 27, a fourth drive motor 33 mounted on the support frame 22, a first bevel gear 34 mounted on the output shaft of the fourth drive motor 33, and the first bevel gear 34 meshing with the second bevel gear 35.

[0033] A protective box 36 is installed at the lower part of the frame 1. The lower part of the double-layer rotary sampling mechanism is located inside the protective box 36, which can prevent the driving components from being affected by the environment and improve the safety of the device, avoiding injury to the user.

[0034] In operation, the third drive motor 29 is activated, which drives the second synchronous pulley 31 to rotate. The rotation of the second synchronous pulley 31, via the synchronous belt 32, drives the first synchronous pulley 30 to rotate. The rotation of the first synchronous pulley 30 drives the rotating seat 23 to rotate, which in turn drives the lower support tray 20 and the upper positioning plate 21 to rotate together. The fourth drive motor 33 is then activated, driving the first bevel gear 34 to rotate. The rotation of the first bevel gear 34 drives the second bevel gear 35 to rotate, which in turn drives the screw 27 to rotate. The screw 27 then drives the lifting tube 28 to rise, which in turn raises the upper positioning plate 21, increasing the distance between the lower support tray 20 and the upper positioning plate 21. During the rise of the upper positioning plate 21, the lifting rod 25 rises along with it. To reduce the distance between the lower support tray 20 and the upper positioning plate 21, the screw 27 is rotated in the opposite direction.

[0035] The working process of this invention is as follows: First, the operator places multiple empty sample tubes 19 into the double-layer rotary sampling mechanism in sequence. The bottom of each sample tube 19 is embedded in the groove 2001 of the lower support tray 20, and the upper part is inserted into the positioning hole 2101 of the upper positioning plate 21.

[0036] Next, open the door 3 of the crushing chamber 2, place the chestnut sample to be tested into the inner rotating cylinder 4, close the door 3, and ensure that the crushing chamber 2 is sealed. Inject a measured amount of sterile diluent into the inner rotating cylinder 4 through the inlet pipe 11 on the door 3.

[0037] Subsequently, the first drive motor 6 drives the inner rotating drum 4 to rotate at a set speed and direction through the meshing transmission of gear 7 and gear ring 5. The rotation of the inner rotating drum 4 generates centrifugal force, causing the material to tumble inside the drum. At the same time, centrifugal force helps the liquid flow out through the filter holes and throws away large particles that may clog the filter holes, achieving self-cleaning and anti-clogging. The second drive motor 8 drives the rotating shaft 9 and the crushing blade 10 to rotate at a set speed and direction. Under the rotation of the inner rotating drum 4 and the shearing action of the crushing blade 10, the chestnut sample is mixed with the solvent and gradually crushed. When the particle diameter is smaller than the filter hole diameter on the inner rotating drum 4, under the combined action of centrifugal force and gravity, the solid-liquid mixture passes through the filter hole and flows into the inner cavity of the crushing chamber 2, eventually collecting in the discharge hopper 12 at the bottom. Large particles that fail to pass through the filter hole are trapped inside the inner rotating drum 4 and continue to be sheared by the crushing blade 10 until they are crushed to the size that can pass through the hole.

[0038] Subsequently, the solid-liquid mixture flowing out of the discharge hopper 12 enters the inlet of the venturi tube 13 below under the action of gravity. When the mixture flows through the throat of the venturi tube 13, the flow velocity increases significantly and the pressure decreases due to the sharp reduction in cross-section. At the same time, the control system activates the high-pressure sterile gas source, and sterile gas is injected at high speed through the high-pressure sterile gas injection pipe 14 along the tangential direction of the throat wall. After the gas is injected tangentially, it immediately forms a high-speed rotating airflow in the throat, which drives the liquid to rotate together, generating a strong vortex. This vortex causes the starch particles to be subjected to centrifugal force, thrown against the pipe wall and immediately dispersed by the airflow, making it impossible for them to adhere or settle. After the intense mixing in the venturi tube 13, the starch particles in the solid-liquid mixture are uniformly dispersed, forming a homogeneous fluid with uniformly suspended solid particles, which flows out from the outlet of the venturi tube 13.

[0039] Subsequently, the homogenized fluid flows out of the Venturi tube 13 outlet and into the tee connector 15. One path flows continuously through the overflow pipe 16, which can return to the crushing chamber 2 or be discharged into the waste liquid collection bottle; the overflow pipe 16 is always kept open to ensure continuous fluid flow within the Venturi tube 13, preventing system damage due to pressure buildup, and ensuring that the liquid in the pipeline remains flowing during non-sampling periods to prevent sedimentation. The other path connects to the clamp valve 17 and then flows out through the sampling needle 18.

[0040] Then, the third drive motor 29 starts, driving the rotating seat 23 to rotate via a synchronous belt pulley, which in turn drives the lower support tray 20 and the upper positioning plate 21 to rotate together. When the first empty sample tube 19 rotates to directly below the sampling needle 18, the third drive motor 29 stops, and the sampling plate is precisely positioned. The clamp valve 17 is opened, and the homogenized fluid flows into the sample tube 19 below through the sampling needle 18. The sampling needle 18 can integrate a liquid level sensor. When the liquid level reaches the set height or the sampling time reaches the preset value, the control system closes the clamp valve 17 and stops the sampling. After the clamp valve 17 is closed, the third drive motor 29 is started again, driving the sampling plate to rotate and move the next empty sample tube 19 to directly below the sampling needle 18. The above steps are repeated until all sample tubes 19 have been sampled. During the period when the clamp valve 17 is closed, all the homogenized fluid flowing out of the venturi tube 13 is discharged through the overflow pipe 16, ensuring continuous operation of the system and stable pressure.

[0041] Finally, the operator can take out the sample tube 19 with the sample added and manually carry out subsequent culture and colony counting, or send the sample tube 19 directly into the automated culture and detection unit that is connected to it.

[0042] After all sampling is completed, the device automatically enters the cleaning program. Cleaning fluid can be injected into the system through the inlet pipe 11 to activate the crushing and homogenizing mechanisms, thoroughly cleaning the internal pipelines. The clamp valve 17 can be opened periodically to allow the cleaning fluid to flow through the sampling needle 18, preventing needle blockage. After cleaning, sterile gas can be introduced to purge the pipelines, drying the system and putting it into standby mode, awaiting the next test.

[0043] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A microbial detection device for deep-processed chestnut products, characterized in that: The machine includes a frame (1), a crushing mechanism is provided on the top of the frame (1), a mixing mechanism is provided below the crushing mechanism, a tee (15) is provided below the mixing mechanism, an overflow pipe (16) and a clamp valve (17) are provided on the other two ports of the tee (15), a sample needle (18) is provided below the clamp valve (17), a double-layer rotary sampling mechanism is provided below the sample needle (18), and a sample tube (19) is provided on the double-layer rotary sampling mechanism.

2. The microbial detection device for deep-processed chestnut products according to claim 1, characterized in that: The crushing mechanism includes a crushing box (2) located on the top of the frame (1). The crushing box (2) has an openable door (3) on its right side. The crushing box (2) contains an inner rotating cylinder (4) and a drive assembly for driving the inner rotating cylinder (4) to rotate. The inner rotating cylinder (4) has several filter holes. The crushing box (2) has a second drive motor (8) located on its outer side. The output shaft of the second drive motor (8) is connected to a rotating shaft (9) located inside the inner rotating cylinder (4). The rotating shaft (9) has a crushing blade (10). The crushing box (2) has a discharge hopper (12) at its bottom.

3. The microbial detection device for deep-processed chestnut products according to claim 2, characterized in that: The box door (3) is equipped with a liquid inlet pipe (11).

4. The microbial detection device for deep-processed chestnut products according to claim 2, characterized in that: The drive assembly includes a gear ring (5) disposed on the outside of the inner rotating drum (4), and a first drive motor (6) is disposed on the outside of the crushing box (2). The output shaft of the first drive motor (6) extends into the crushing box (2) and is connected to a gear (7). The gear (7) meshes with the gear ring (5).

5. The microbial detection device for deep-processed chestnut products according to claim 2, characterized in that: The mixing mechanism includes a mixing tube disposed below the discharge hopper (12), the mixing tube having a fluid inlet and a fluid outlet, and a fluid channel connecting the fluid inlet and the fluid outlet, the fluid channel including at least one acceleration section, the cross-sectional area of ​​the acceleration section being smaller than the cross-sectional area of ​​the fluid inlet and / or the fluid outlet; at least one high-pressure sterile gas injection pipe (14) is provided on the wall of the mixing tube.

6. The microbial detection device for deep-processed chestnut products according to claim 5, characterized in that: The mixing tube is configured as a Venturi tube (13), and at least one high-pressure sterile gas injection tube (14) is provided on the throat wall of the Venturi tube (13).

7. The microbial detection device for deep-processed chestnut products according to claim 6, characterized in that: The high-pressure sterile gas injection pipe (14) is arranged along the tangential direction of the throat wall of the venturi tube (13).

8. The microbial detection device for deep-processed chestnut products according to claim 1, characterized in that: The double-layer rotary sampling mechanism includes a lower support tray (20) and an upper positioning plate (21). The surface of the lower support tray (20) is provided with a groove (2001) that matches the bottom of the sample tube (19). The upper positioning plate (21) is provided with a positioning hole (2101) that matches the outer diameter of the sample tube (19). The distance between the lower support tray (20) and the upper positioning plate (21) is adjustable to match the sample tubes (19) of different heights. A rotary drive assembly for driving the lower support tray (20) and the upper positioning plate (21) to rotate is provided below the lower support tray (20).

9. The microbial detection device for deep-processed chestnut products according to claim 8, characterized in that: The rotary drive assembly includes a rotary seat (23) disposed on the top of the support frame (22), the lower support tray (20) and the upper positioning plate (21) are disposed above the rotary seat (23), and a first drive structure for driving the rotary seat (23) to rotate is disposed on the outside of the rotary seat (23). The drive structure includes a first synchronous pulley (30) disposed on the outside of the rotating seat (23), a third drive motor (29) disposed on the support frame (22), a second synchronous pulley (31) disposed on the output shaft of the third drive motor (29), and the first synchronous pulley (30) and the second synchronous pulley (31) being rotatably connected by a synchronous belt (32).

10. The microbial detection device for deep-processed chestnut products according to claim 9, characterized in that: The top surface of the rotating seat (23) is provided with several fixed tubes (24), and a lifting rod (25) is provided above the fixed tubes (24). An upper positioning plate (21) is provided on the top surface of the lifting rod (25). A lower support plate (20) is provided on the outside of the fixed tubes (24). A screw (27) is provided at the center of the rotating seat (23). A second driving structure for driving the screw (27) to rotate is provided on the support frame (22). A lifting tube (28) is provided at the center of the bottom surface of the upper positioning plate (21). A thread matching the screw (27) is provided inside the lifting tube (28). A fixing sleeve (26) is provided at the center of several fixed tubes (24). The lifting tube (28) moves along the fixing sleeve (26). The second drive structure includes a second bevel gear (35) disposed at the bottom of the screw (27), a fourth drive motor (33) disposed on the support frame (22), a first bevel gear (34) disposed on the output shaft of the fourth drive motor (33), and the first bevel gear (34) meshing with the second bevel gear (35).

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