A biocontrol fungal spore cultivation device for controlling Japanese pine scale and its usage method

Abstract

This invention discloses a biocontrol spore cultivation device and its usage method for controlling Japanese pine scale, belonging to the field of pine scale control technology. The device includes an inoculation unit and several cultivation units. The inoculation unit includes a frame with a chain conveyor inside. Multiple positioning support plates are evenly spaced on the chain conveyor. A pre-compression mechanism and an inoculation mechanism are located directly above the chain conveyor on the frame, both spanning the chain conveyor. Several cultivation units are located at the outlet of the inoculation unit. This invention achieves automatic inoculation of biocontrol spores, improving inoculation efficiency and enabling precise control of the inoculation amount, thus improving the uniformity and consistency of biocontrol spore concentration. Next, the inoculated bottles are transferred to multiple cultivation units for static cultivation to improve the viability and yield of biocontrol spores, meeting the needs of large-scale production.

Description

Technical Field

[0001] This invention relates to a biocontrol fungal spore cultivation device and its usage method for controlling Japanese pine scale, belonging to the field of pine scale control technology. Background Technology

[0002] The Japanese pine scale insect, a devastating forest pest, primarily parasitizes the bark of pine trees, sucking sap and causing weakened growth, branch dieback, and even the death of the entire tree, posing a serious threat to pine forest ecosystems and the forestry economy. While chemical control can manage the pest in the short term, it easily causes environmental pollution, kills natural enemies, and long-term use can lead to pesticide resistance, contradicting the concept of green pest control. Biological control, with its environmentally friendly and sustainable advantages, has become the core direction for controlling the Japanese pine scale insect. Among these methods, biological agents made from the spores of biocontrol bacteria (such as Beauveria bassiana and Metarhizium anisopliae) can precisely kill the Japanese pine scale insect through parasitic or pathogenic action, making it one of the most promising control methods currently available.

[0003] However, the large-scale cultivation of biocontrol spores for the control of Japanese pine scale faces many technical bottlenecks: traditional cultivation methods rely on manual inoculation and static culture, which not only has low inoculation efficiency but also makes it difficult to accurately control the inoculation amount, resulting in uneven concentration of biocontrol spores and affecting the control effect; the environmental parameter control during the cultivation process is rough, and it is easy to cause low viability and unstable yield of biocontrol spores due to contamination by miscellaneous bacteria or unsuitable conditions, making it difficult to meet the needs of large-scale production. Summary of the Invention

[0004] To solve the above-mentioned technical problems, the present invention provides a biocontrol fungal spore cultivation device for the control of Japanese pine scale and its usage method.

[0005] This invention is achieved through the following technical solution: A biocontrol spore cultivation device for controlling Japanese pine scale includes an inoculation device and several cultivation devices. The inoculation device includes a frame with a chain conveyor inside. Multiple positioning support plates are evenly spaced on the chain conveyor. A pre-compression mechanism and an inoculation mechanism are located directly above the chain conveyor on the frame, and both the pre-compression mechanism and the inoculation mechanism span the chain conveyor. Several cultivation devices are located at the discharge port of the inoculation device.

[0006] The positioning support plate is connected to the two chains of the chain conveyor. The positioning support plate has multiple circular positioning grooves, and the line connecting the centers of all the circular positioning grooves is arranged along the width direction of the chain conveyor.

[0007] The pre-compression mechanism includes two pre-compression cylinders arranged side by side. The lower ends of the two pre-compression cylinders are connected by a pre-compression plate, and the upper ends are connected to the frame by mounting bases. The pre-compression plate is provided with multiple through holes.

[0008] The inoculation mechanism includes an inoculation cylinder and a square frame. The upper end of the inoculation cylinder is connected to the frame, and the top of the square frame is connected to the lower end of the inoculation cylinder. Two guide posts are arranged side by side on the top of the square frame, and both guide posts are slidably connected to the frame. Multiple inoculation needles are provided at the lower part of the square frame, and the central axis of the multiple inoculation needles is collinear with the central axis of multiple through holes on the pre-pressing plate. A liquid storage tank and multiple metering pumps are provided inside the square frame. The inlets of the multiple metering pumps are connected to the inside of the liquid storage tank through inlet pipes, and the outlets of the multiple metering pumps are connected to the upper ends of the multiple inoculation needles through outlet pipes. A pressure sensor is located at the bottom of the box near each inoculation needle.

[0009] It also includes solenoid valve A, solenoid valve B and controller. Solenoid valve A is connected to two pre-pressurized cylinders through air pipe A, solenoid valve B is connected to the inoculation cylinder through air pipe B, and controller is electrically connected to solenoid valve A, solenoid valve B, chain conveyor, metering pump and pressure sensor.

[0010] The culture device is a constant temperature and humidity incubator, and the constant temperature and humidity incubator is equipped with a bottle inversion mechanism.

[0011] The bottle inversion mechanism includes a bottle clamping assembly and an inversion drive assembly. A support frame is provided inside the constant temperature and humidity incubator. The bottle clamping assembly is located on the support frame. One end of the inversion drive assembly is connected to the support frame, and the other end is connected to the bottle clamping assembly.

[0012] The bottle clamping assembly includes a top plate and a support plate arranged side by side. Both ends of the top plate are connected to a rotating shaft, and the rotating shafts at both ends of the top plate are respectively mounted on the top of the support frame through bearing seats. Multiple clamping cylinders are installed on the top plate, and pressure blocks are connected to the piston rods of the multiple clamping cylinders. The support plate is connected to the top plate through two connecting rods A arranged side by side. Multiple positioning rings are provided on the support plate. The multiple positioning rings are aligned with the pressure blocks on the multiple clamping cylinders one by one, and the positioning rings and pressure blocks are located between the top plate and the support plate. The inverted drive assembly includes a support shaft, an inverted cylinder, and a rocker arm. One end of the support shaft is fixedly connected to a mounting plate, which is mounted on a support frame. One end of the inverted cylinder is rotatably connected to the support shaft via a single lug, and the other end is connected to a double lug, which is equipped with a connecting shaft. One end of the rocker arm is fitted onto a rotating shaft at one end of the top plate and connected to a rotating shaft key, while the other end is rotatably connected to the connecting shaft.

[0013] The bottle inverting mechanism also includes an auxiliary support assembly, which includes a support plate located between the top plate and the support plate. Two connecting rods B are arranged side by side on the support plate, and the two connecting rods B are connected to two connecting rods A one-to-one through connecting blocks. Multiple auxiliary support rods are arranged side by side on the support plate between the two connecting rods B, and one end of each auxiliary support rod is provided with a V-shaped auxiliary support plate.

[0014] A method for using a biocontrol fungal spore cultivation device for controlling Japanese pine scale includes the following steps: Step 1: Add the sterilized PDA culture medium quantitatively to the sterile bottle, place the bottle in a sterile environment to cool to a suitable temperature, and immediately cover the bottle opening with a sterile rubber stopper and let it cool to room temperature. Step 2: Start the constant temperature and humidity incubator and set its temperature to 25±1℃ and relative humidity to >70%, then start the chain conveyor; Step 3: Place the bottle with the rubber stopper prepared in Step 1 onto the positioning support plate at the feed inlet of the chain conveyor, and then move the positioning support plate on the chain conveyor back one station. Step 4: The pre-compression mechanism activates, pressing down the rubber stoppers on all the bottles on the positioning support plate directly below it. Then, the inoculation mechanism activates, inoculating the PDA culture medium in all the bottles on the positioning support plate directly below it with biocontrol spores. Then, the inoculation mechanism and the pre-compression mechanism reset one after the other. Step 5: Repeat steps 3 and 4, and inoculate the PDA culture medium in the bottles on each positioning support plate with biocontrol spores in turn; Step 6: Transfer the inoculated vials to the inverting mechanism inside the constant temperature and humidity incubator. Then, clamp the vials with the inverting mechanism and invert them to begin the static culture of biocontrol spores.

[0015] The beneficial effects of this invention are as follows: 1. The feed bottles are supported by a positioning support plate and radially limited. A chain conveyor transports the bottles directly below the pre-compression and inoculation mechanisms. The pre-compression mechanism first presses down the rubber stopper at the bottle opening, and then the inoculation mechanism quantitatively inoculates biocontrol spores onto the PDA culture medium inside the bottle. This achieves automated inoculation of biocontrol spores, improving inoculation efficiency and allowing for precise control of the inoculation amount, thus enhancing the uniformity and consistency of spore concentration and ensuring its control effect against Japanese pine scale. Next, the inoculated bottles are transferred to multiple culture devices for static cultivation. These devices provide the necessary temperature and humidity for spore cultivation, improving spore viability and yield to meet the needs of large-scale production.

[0016] 2. When a positioning support plate moves directly below the pre-compression mechanism and the inoculation mechanism, the chain conveyor stops running, and the two pre-compression cylinders act simultaneously, pushing the pre-compression plate to press down the rubber stopper at the bottle opening on the positioning support plate: on the one hand, this can prevent the bottle from shaking when the inoculation needle punctures the rubber stopper; on the other hand, it can prevent the rubber stopper from moving upwards and detaching from the bottle when the inoculation needle is pulled out.

[0017] 3. When the pre-compression plate in the pre-compression mechanism moves down and presses down on the rubber stopper at the bottle opening on its lower positioning support plate, the inoculation cylinder pushes the square frame down until the lower end of the inoculation needle passes through the through hole and pierces the rubber stopper at the bottle opening. Then, the metering pump starts, quantitatively injecting biocontrol spores from the storage tank into the bottle, thus achieving quantitative inoculation of biocontrol spores onto the PDA culture medium. Because the biocontrol spores are inoculated in a closed, sterile environment enclosed by the bottle and the rubber stopper, the risk of contamination by other microorganisms is greatly reduced.

[0018] 4. The pressure sensor, inoculation needle, and metering pump are configured in a one-to-one correspondence. When a pressure sensor is triggered, it transmits a signal to the controller, which then activates the corresponding metering pump to inject biocontrol spores into the container in a measured quantity. If a pressure sensor is not triggered, the corresponding metering pump does not start. Therefore, by placing pressure sensors near each inoculation needle, and ensuring that no container is placed in any of the circular positioning grooves on the positioning support plate directly below the pre-compression and inoculation mechanisms, the corresponding metering pump can be prevented from erroneously starting, thus avoiding waste of biocontrol spores. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a top view of the structure of the present invention; Figure 3 for Figure 2 Sectional view along AA; Figure 4 This is a schematic diagram of the rear view structure of the present invention; Figure 5 for Figure 4 A cross-sectional view along BB; Figure 6 for Figure 5 A magnified view of a portion at point A; Figure 7 This is a schematic diagram of the pre-compression mechanism of the present invention; Figure 8 This is a schematic diagram of the inoculation mechanism of the present invention after the inoculation cylinder has been removed; Figure 9 This is a schematic diagram of the assembly structure of the inverted bottle mechanism, support frame, and bottom plate of the constant temperature and humidity incubator of the present invention. Figure 10 for Figure 9 A structural diagram from another perspective.

[0020] In the diagram: 1-Frame, 4-Pre-compression mechanism, 41-Pre-compression plate, 411-Through hole, 42-Pre-compression cylinder, 43-Mounting base, 5-Inoculation mechanism, 51-Inoculation cylinder, 52-Guide column, 53-Frame, 54-Liquid storage tank, 55-Metering pump, 56-Inoculation needle, 57-Inlet pipe, 58-Outlet pipe, 59-Pressure sensor, 6-Variety bottle, 7-Chain conveyor, 8-Positioning support plate, 9-Circular positioning groove, 10-Constant temperature and humidity incubator, 101-Support frame, 11-Variety bottle inversion mechanism, 12-Inversion drive Components: 121-Support shaft, 122-Inverted cylinder, 123-Double ear seat, 124-Connecting shaft, 125-Rock arm, 126-Mounting plate, 13-Bottle clamping assembly, 131-Rotating shaft, 132-Connecting rod A, 133-Clamping cylinder, 134-Top plate, 135-Bearing with seat, 136-Pressure block, 137-Support plate, 138-Positioning ring, 14-Auxiliary support assembly, 141-Support plate, 142-Connecting rod B, 143-Connecting block, 144-Auxiliary support rod, 145-V-shaped auxiliary support plate. Detailed Implementation

[0021] The technical solution of the present invention is further described below, but the scope of protection is not limited to what is described.

[0022] like Figures 1 to 10 As shown, the present invention discloses a biocontrol fungal spore cultivation device for controlling Japanese pine scale, comprising an inoculation device and multiple cultivation devices. The inoculation device includes a frame 1, a chain conveyor 7 is provided inside the frame 1, and multiple positioning support plates 8 are provided at equal intervals on the chain conveyor 7. A pre-compression mechanism 4 and an inoculation mechanism 5 are provided on the frame 1 directly above the chain conveyor 7, and both the pre-compression mechanism 4 and the inoculation mechanism 5 span the chain conveyor 7. The multiple cultivation devices are all located at the discharge port of the inoculation device.

[0023] Specifically, the material bottle 6 is supported by a positioning support plate 8, which radially limits its movement. A chain conveyor 7 transports the material bottle 6 directly below the pre-compression mechanism 4 and the inoculation mechanism 5. The pre-compression mechanism 4 first presses down the rubber stopper at the bottle opening, and then the inoculation mechanism 5 quantitatively inoculates biocontrol spores onto the PDA culture medium inside the material bottle 6. This achieves automated inoculation of biocontrol spores, improving inoculation efficiency and allowing for precise control of the inoculation amount, thus enhancing the uniformity and consistency of biocontrol spore concentration and ensuring its control effect against Japanese pine scale. Next, the inoculated material bottle 6 is transferred to multiple culture devices for static cultivation. These devices provide the necessary temperature and humidity for biocontrol spore cultivation, improving spore viability and yield to meet the needs of large-scale production.

[0024] Because the inoculation device is highly efficient and requires little time to inoculate biocontrol spores, while the culture period for biocontrol spores in the culture device is relatively long, equipping each inoculation device with multiple culture devices can significantly increase the yield of biocontrol spores.

[0025] The positioning support plate 8 is connected to the two chains of the chain conveyor 7. The positioning support plate 8 has multiple circular positioning grooves 9, and the line connecting the centers of all the circular positioning grooves 9 is arranged along the width direction of the chain conveyor 7.

[0026] Specifically, the diameter of the circular positioning groove 9 is adapted to the outer diameter of the bottle 6 so as to radially limit the bottle 6 through the circular positioning groove 9, and ensure that when the bottle 6 moves to the underside of the pre-pressing mechanism 4 and the inoculation mechanism 5, it can be aligned with the through hole 411 on the pre-pressing plate 41 and the inoculation needle 56 on the frame 53.

[0027] The pre-compression mechanism 4 includes two pre-compression cylinders 42 arranged side by side. The lower ends of the two pre-compression cylinders 42 are connected by a pre-compression plate 41, and the upper ends are connected to the frame 1 by mounting bases 43 respectively. The pre-compression plate 41 is provided with multiple through holes 411.

[0028] Specifically, when a positioning support plate 8 moves directly below the pre-compression mechanism 4 and the inoculation mechanism 5, the chain conveyor 7 stops running, and the two pre-compression cylinders 42 operate simultaneously, pushing the pre-compression plate 41 to press down the rubber stopper at the mouth of the feeding bottle 6 on the positioning support plate 8: on the one hand, this can prevent the feeding bottle 6 from shaking when the inoculation needle 56 punctures the rubber stopper; on the other hand, it can prevent the rubber stopper from moving upward and detaching from the feeding bottle 6 when the inoculation needle 56 is pulled out.

[0029] The inoculation mechanism 5 includes an inoculation cylinder 51 and a frame 53. The upper end of the inoculation cylinder 51 is connected to the frame 1, and the top of the frame 53 is connected to the lower end of the inoculation cylinder 51. Two guide posts 52 are arranged side by side on the top of the frame 53, and both guide posts 52 are slidably connected to the frame 1. Multiple inoculation needles 56 are provided at the lower part of the frame 53, and the central axis of the multiple inoculation needles 56 is collinear with the central axis of the multiple through holes 411 on the pre-pressure plate 41. The inner side of the frame 53 is provided with a liquid storage tank 54 and multiple metering pumps 55. The inlets of the multiple metering pumps 55 are respectively connected to the inside of the liquid storage tank 54 through inlet pipes 57, and the outlets of the multiple metering pumps 55 are respectively connected to the upper ends of the multiple inoculation needles 56 through outlet pipes 58. Pressure sensors 59 are provided at the bottom of the frame 53 near each inoculation needle 56.

[0030] Specifically, when the pre-compression plate 41 in the pre-compression mechanism 4 moves down and presses down on the rubber stopper at the mouth of the bottle 6 on the positioning support plate 8 below it, the inoculation cylinder 51 pushes the square frame 53 down until the lower end of the inoculation needle 56 passes through the through hole 411 and pierces the rubber stopper at the mouth of the bottle 6. Then, the metering pump 55 starts, quantitatively injecting the biocontrol spores in the storage tank 54 into the bottle 6, realizing quantitative inoculation of biocontrol spores on the PDA culture medium. Since the biocontrol spores are inoculated in a closed sterile environment surrounded by the bottle 6 and the rubber stopper, the risk of contamination by other microorganisms can be greatly reduced.

[0031] Furthermore, the pressure sensor 59, inoculation needle 56, and metering pump 55 are configured in a one-to-one correspondence. When a pressure sensor 59 is triggered, it transmits a relevant signal to the controller, which then controls the corresponding metering pump 55 to start, quantitatively injecting biocontrol spores into the container 6. When a pressure sensor 59 is not triggered, the corresponding metering pump 55 does not start. Therefore, by placing pressure sensors 59 near each inoculation needle 56, when no container 6 is placed in some or all of the circular positioning grooves 9 on the positioning support plate 8 directly below the pre-compression mechanism 4 and the inoculation mechanism 5, the corresponding metering pump 55 can be prevented from erroneously starting, thus avoiding waste of biocontrol spores.

[0032] It also includes solenoid valve A, solenoid valve B, and a controller. Solenoid valve A is connected to two pre-pressurized cylinders 42 via air pipe A, and solenoid valve B is connected to inoculation cylinder 51 via air pipe B. The controller is electrically connected to solenoid valve A, solenoid valve B, chain conveyor 7, metering pump 55, and pressure sensor 59. The controller provides overall control of the inoculation device.

[0033] The cultivation device is a constant temperature and humidity incubator 10, and the constant temperature and humidity incubator 10 is equipped with a material bottle inversion mechanism 11. The constant temperature and humidity incubator 10 provides the required temperature and relative humidity for the cultivation of biocontrol fungal spores.

[0034] The bottle inversion mechanism 11 includes a bottle clamping assembly 13 and an inversion drive assembly 12. A support frame 101 is provided inside the constant temperature and humidity incubator 10. The bottle clamping assembly 13 is located on the support frame 101. One end of the inversion drive assembly 12 is connected to the support frame 101, and the other end is connected to the bottle clamping assembly 13.

[0035] Specifically, after the bottle 6 with the rubber stopper is clamped onto the bottle clamping assembly 13, the bottle clamping assembly 13 and the bottle 6 are rotated together by the inverting drive assembly 12 until the bottle 6 is inverted.

[0036] The bottle clamping assembly 13 includes a top plate 134 and a support plate 137 arranged side by side. Both ends of the top plate 134 are connected to a rotating shaft 131, and the rotating shafts 131 at both ends of the top plate 134 are respectively mounted on the top of the support frame 101 through bearings 135. Multiple clamping cylinders 133 are mounted on the top plate 134, and pressure blocks 136 are connected to the piston rods of the multiple clamping cylinders 133. The support plate 137 is connected to the top plate 134 through two connecting rods A132 arranged side by side. Multiple positioning rings 138 are provided on the support plate 137. The multiple positioning rings 138 are aligned with the pressure blocks 136 on the multiple clamping cylinders 133, and the positioning rings 138 and pressure blocks 136 are located between the top plate 134 and the support plate 137. The inverted drive assembly 12 includes a support shaft 121, an inverted cylinder 122, and a rocker arm 125. One end of the support shaft 121 is fixedly connected to a mounting plate 126, which is mounted on a support frame 101. One end of the inverted cylinder 122 is rotatably connected to the support shaft 121 via a single ear seat, and the other end is connected to a double ear seat 123, which is provided with a connecting shaft 124. One end of the rocker arm 125 is fitted onto a rotating shaft 131 at one end of the top plate 134 and is keyed to the rotating shaft 131, while the other end is rotatably connected to the connecting shaft 124.

[0037] Specifically, the process of clamping the bottle with rubber stopper 6 by the bottle clamping assembly 13 is as follows: the bottom of the bottle 6 is radially limited by the positioning ring 138, and then the rubber stopper is pressed by the clamping cylinder 133 and the pressing block 136, thereby axially limiting the bottle with rubber stopper 6 by the pressing block 136 and the support plate 137, so as to realize the clamping of the bottle with rubber stopper 6.

[0038] The process by which the inverting drive assembly 12 inverts the stoppered bottle 6 is as follows: Figure 9 As shown, the inverted cylinder 122 extends and pushes the rotating shaft 131 to rotate at a certain angle through the double ear seat 123, connecting shaft 124 and rocker arm 125. At the same time, the rotating shaft 131 drives the bottle clamping assembly 13 and the bottle 6 to rotate together until the bottle 6 is inverted, that is, the bottle mouth of the bottle 6 tilts downward.

[0039] The bottle inverting mechanism 11 also includes an auxiliary support assembly 14, which includes a support plate 141 located between the top plate 134 and the support plate 137. Two connecting rods B142 are arranged side by side on the support plate 141, and the two connecting rods B142 are connected to the two connecting rods A132 one by one through the connecting block 143. Multiple auxiliary support rods 144 are arranged side by side on the support plate 141 between the two connecting rods B142, and one end of the auxiliary support rod 144 is provided with a V-shaped auxiliary support plate 145.

[0040] Specifically, the bottle inversion mechanism 11 supports the outer circle of the bottle 6 with rubber stopper, thereby improving the clamping stability and reliability of the bottle inversion mechanism 11 on the bottle 6 with rubber stopper.

[0041] A method for using a biocontrol fungal spore cultivation device for controlling Japanese pine scale includes the following steps: Step 1: Add the sterilized PDA culture medium quantitatively to sterile bottle 6, and place bottle 6 in a sterile environment to cool to a suitable temperature. Immediately afterward, cover the bottle opening with a sterile rubber stopper and allow it to cool to room temperature. Step 2: Start the constant temperature and humidity incubator 10 and set its temperature to 25±1℃ and relative humidity to >70%, then start the chain conveyor 7.

[0042] Step 3: Place the bottle 6 with the rubber stopper prepared in Step 1 onto the positioning support plate 8 at the feed inlet of the chain conveyor 7. Then, the positioning support plate 8 on the chain conveyor 7 moves backward one station. The chain conveyor 7 performs intermittent motion and is driven by a servo motor or stepper motor.

[0043] Step 4: The pre-compression mechanism 4 is activated, pressing down the rubber stoppers on all the bottles 6 on the positioning support plate 8 directly below it. Then the inoculation mechanism 5 is activated, inoculating the PDA culture medium in all the bottles 6 on the positioning support plate 8 directly below it with biocontrol spores. Then the inoculation mechanism 5 and the pre-compression mechanism 4 are reset one after the other.

[0044] Step 5: Repeat steps 3 and 4, and inoculate the PDA culture medium in the bottle 6 on each positioning support plate 8 with biocontrol spores.

[0045] Step 6: Transfer the inoculated culture bottle 6 to the culture bottle inversion mechanism 11 inside the constant temperature and humidity incubator 10. Then, clamp the culture bottle 6 with the culture bottle inversion mechanism 11 and invert the culture bottle 6 to start the static culture of biocontrol spores.

Claims

1. A biocontrol spore cultivation device for controlling Japanese pine scale, characterized in that: The device includes an inoculation device and several culture devices. The inoculation device includes a frame (1), a chain conveyor (7) is provided inside the frame (1), and multiple positioning support plates (8) are provided at equal intervals on the chain conveyor (7). A pre-compression mechanism (4) and an inoculation mechanism (5) are provided on the frame (1) directly above the chain conveyor (7), and both the pre-compression mechanism (4) and the inoculation mechanism (5) span the chain conveyor (7). Several culture devices are located at the discharge port of the inoculation device.

2. The biocontrol spore cultivation equipment for controlling Japanese pine scale as described in claim 1, characterized in that: The positioning support plate (8) is connected to the two chains of the chain conveyor (7). The positioning support plate (8) has multiple circular positioning grooves (9), and the center line of all the circular positioning grooves (9) is arranged along the width direction of the chain conveyor (7).

3. The biocontrol spore cultivation equipment for controlling Japanese pine scale as described in claim 1, characterized in that: The pre-compression mechanism (4) includes two pre-compression cylinders (42) arranged side by side. The lower ends of the two pre-compression cylinders (42) are connected by a pre-compression plate (41), and the upper ends are connected to the frame (1) by mounting bases (43). The pre-compression plate (41) is provided with multiple through holes (411).

4. The biocontrol spore cultivation equipment for controlling Japanese pine scale as described in claim 3, characterized in that: The inoculation mechanism (5) includes an inoculation cylinder (51) and a square frame (53). The upper end of the inoculation cylinder (51) is connected to the frame (1), and the top of the square frame (53) is connected to the lower end of the inoculation cylinder (51). Two guide posts (52) are arranged side by side on the top of the square frame (53), and both guide posts (52) are slidably connected to the frame (1). Multiple inoculation needles (56) are provided at the lower part of the square frame (53), and the central axis of the multiple inoculation needles (56) is collinear with the central axis of the multiple through holes (411) on the pre-press plate (41). A liquid storage tank (54) and multiple metering pumps (55) are provided on the inner side of the square frame (53). The inlet of the multiple metering pumps (55) is connected to the inside of the liquid storage tank (54) through the inlet pipe (57), and the outlet of the multiple metering pumps (55) is connected to the upper end of the multiple inoculation needles (56) through the outlet pipe (58). The bottom of the box (53) is provided with a pressure sensor (59) near each inoculation needle (56).

5. The biocontrol spore cultivation equipment for controlling Japanese pine scale insects as described in claim 4, characterized in that: It also includes solenoid valve A, solenoid valve B and controller. Solenoid valve A is connected to two pre-pressurized cylinders (42) through air pipe A. Solenoid valve B is connected to inoculation cylinder (51) through air pipe B. Controller is electrically connected to solenoid valve A, solenoid valve B, chain conveyor (7), metering pump (55) and pressure sensor (59).

6. The biocontrol spore cultivation equipment for controlling Japanese pine scale insects as described in claim 1, characterized in that: The culture device is a constant temperature and humidity incubator (10), and the constant temperature and humidity incubator (10) is equipped with a bottle inversion mechanism (11).

7. The biocontrol spore cultivation equipment for controlling Japanese pine scale insects as described in claim 6, characterized in that: The bottle inversion mechanism (11) includes a bottle clamping assembly (13) and an inversion drive assembly (12). A support frame (101) is provided inside the constant temperature and humidity incubator (10). The bottle clamping assembly (13) is located on the support frame (101). One end of the inversion drive assembly (12) is connected to the support frame (101), and the other end is connected to the bottle clamping assembly (13).

8. The biocontrol spore cultivation equipment for controlling Japanese pine scale insects as described in claim 7, characterized in that: The bottle clamping assembly (13) includes a top plate (134) and a support plate (137) arranged side by side. Both ends of the top plate (134) are connected to a rotating shaft (131), and the rotating shafts (131) at both ends of the top plate (134) are respectively mounted on the top of the support frame (101) through bearings (135). Multiple clamping cylinders (133) are mounted on the top plate (134), and pressure blocks (136) are connected to the piston rods of the multiple clamping cylinders (133). The support plate (137) is connected to the top plate (134) through two connecting rods A (132) arranged side by side. Multiple positioning rings (138) are provided on the support plate (137). The multiple positioning rings (138) are aligned with the pressure blocks (136) on the multiple clamping cylinders (133), and the positioning rings (138) and pressure blocks (136) are located between the top plate (134) and the support plate (137). The inverted drive assembly (12) includes a support shaft (121), an inverted cylinder (122), and a rocker arm (125). One end of the support shaft (121) is fixedly connected to a mounting plate (126), and the mounting plate (126) is mounted on a support frame (101). One end of the inverted cylinder (122) is rotatably connected to the support shaft (121) via a single ear seat, and the other end is connected to a double ear seat (123). A connecting shaft (124) is provided on the double ear seat (123). One end of the rocker arm (125) is fitted onto a rotating shaft (131) at one end of the top plate (134) and is keyed to the rotating shaft (131). The other end is rotatably connected to the connecting shaft (124).

9. The biocontrol spore cultivation equipment for controlling Japanese pine scale insects as described in claim 8, characterized in that: The bottle inverting mechanism (11) also includes an auxiliary support assembly (14), which includes a support plate (141). The support plate (141) is located between the top plate (134) and the support plate (137). Two connecting rods B (142) are arranged side by side on the support plate (141). The two connecting rods B (142) are connected to the two connecting rods A (132) one by one through the connecting block (143). Multiple auxiliary support rods (144) are arranged side by side on the support plate (141) between the two connecting rods B (142), and one end of the auxiliary support rod (144) is provided with a V-shaped auxiliary support plate (145).

10. A method of using a biocontrol spore cultivation device for controlling Japanese pine scale as described in any one of claims 6 to 9, characterized in that: Includes the following steps: Step 1: Add the sterilized PDA culture medium quantitatively into the sterile bottle (6), and place the bottle (6) in a sterile environment to cool to a suitable temperature. Immediately afterward, cover the bottle (6) with a sterile rubber stopper and wait for it to cool to room temperature. Step 2: Start the constant temperature and humidity incubator (10) and set its temperature to 25±1℃ and relative humidity to >70%, then start the chain conveyor (7). Step 3: Place the bottle (6) with rubber stopper prepared in Step 1 onto the positioning support plate (8) at the feed inlet of the chain conveyor (7), and then move the positioning support plate (8) on the chain conveyor (7) one station backward. Step 4: The pre-compression mechanism (4) is activated, pressing down the rubber stoppers on all the bottles (6) on the positioning support plate (8) directly below it. Then the inoculation mechanism (5) is activated, inoculating the PDA culture medium in all the bottles (6) on the positioning support plate (8) directly below it with biocontrol spores. Then the inoculation mechanism (5) and the pre-compression mechanism (4) are reset one after the other. Step 5: Repeat steps 3 and 4, and inoculate the PDA culture medium in the bottle (6) on each positioning support plate (8) with biocontrol spores in turn; Step 6: Transfer the inoculated vial (6) to the vial inversion mechanism (11) in the constant temperature and humidity incubator (10), then clamp the vial (6) with the vial inversion mechanism (11) and invert the vial (6) to start the static culture of biocontrol spores.

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