Enterobacter cloacae strain STR5 and application thereof
By screening Enterobacter cloacae STR5 to prepare a highly efficient lipase, the problem of tocopherol succinate hydrolysis was solved, and tocopherol was generated efficiently, replacing the traditional chemical method and making it suitable for the green industrial production of tocopherol.
CN122128175APending Publication Date: 2026-06-02QUFU NORMAL UNIV +1
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- QUFU NORMAL UNIV
- Filing Date
- 2026-04-09
- Publication Date
- 2026-06-02
AI Technical Summary
Technical Problem
In existing technologies, the hydrolysis of tocopherol succinate is difficult, traditional chemical methods cause serious pollution, and enzyme-catalyzed screening methods are costly and have a low detection rate of positive strains, making the preparation of tocopherol difficult.
Method used
A strain of Enterobacter cloacae STR5 was screened and provided. A highly efficient lipase was prepared by fermentation to catalyze the hydrolysis of tocopherol succinate under mild reaction conditions to generate tocopherol.
Benefits of technology
This method achieves highly efficient catalytic hydrolysis of tocopherol succinate, increasing the yield of tocopherol by 700%, replacing traditional chemical methods, and is suitable for the green industrial production of tocopherol.
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Abstract
This invention discloses a strain of *Enterobacter cloacae* STR5 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* STR5, with accession number CCTCC NO: M 2025607. This strain exhibits a hydrolysis zone HC value of 3.95 on tributyrate plates and a lipase activity of 121.53 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting strain STR5 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 700% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.
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