Enterobacter cloacae EC11 and application thereof

By screening Enterobacter cloacae EC11 to prepare lipase, which was then applied to the hydrolysis reaction of tocopherol succinate, the environmental pollution problem of traditional chemical hydrolysis methods was solved, and efficient and environmentally friendly tocopherol preparation was achieved.

CN122128176APending Publication Date: 2026-06-02QUFU NORMAL UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QUFU NORMAL UNIV
Filing Date
2026-04-09
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

The traditional chemical hydrolysis method for preparing free tocopherol has problems such as harsh reaction conditions, numerous side reactions, and serious environmental pollution, necessitating the development of milder and more environmentally friendly alternative technologies.

Method used

A strain of Enterobacter cloacae EC11 was screened out, and its lipase was prepared by fermentation. It was then used to catalyze the hydrolysis of tocopherol succinate. The catalysis was carried out under mild conditions using organic solvents and crude enzyme solution.

Benefits of technology

This method achieves highly efficient catalytic hydrolysis of tocopherol succinate to produce tocopherol. It features high catalytic efficiency, is environmentally friendly, and is suitable for the green industrial production of tocopherol.

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Abstract

This invention discloses a strain of *Enterobacter cloacae* EC11 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* EC11, with the accession number CCTCC NO:M 2025610. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate agar plates and a lipase activity of 145.28 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting the lipase strain EC11 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 725% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.
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