A Bg.MEW_38304 gene regulating plant growth and flowering, its encoded protein, and its applications.

By cloning and validating the Bg.MEW_38304 gene, the problem of unclear key regulatory genes in the development of bougainvillea bracts was solved, enabling the improvement of bract traits and the regulation of flowering characteristics, thereby enhancing the economic benefits of ornamental plants.

CN122128325APending Publication Date: 2026-06-02HUNAN PROVINCIAL BOTANICAL GARDEN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUNAN PROVINCIAL BOTANICAL GARDEN
Filing Date
2026-03-20
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing technologies lack clear identification and functional verification methods for key regulatory genes during the development of bougainvillea bracts, especially the insufficient research on the function of SBP family members in the development of bougainvillea bracts, resulting in a lack of effective molecular targets and technical pathways for molecular breeding of ornamental plants.

Method used

The Bg.MEW_38304 gene was cloned and validated. Its function in the development of bracts of *Prunus glabra* was clarified through transcriptome screening, cloning, subcellular localization, genetic transformation, and virus-induced gene silencing. A systematic functional validation technology system was established and applied to the improvement of bract traits and the regulation of flowering characteristics in heterologous plants.

Benefits of technology

The key regulatory genes for bract development in Bougainvillea glabra were identified, which promoted the plant height and flowering quantity of Arabidopsis thaliana, provided technical support for molecular breeding of ornamental plants, and enhanced the economic and application value of bract-bearing ornamental plants.

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Abstract

This invention relates to the Bg.MEW_38304 gene regulating plant growth and flowering, its encoded protein, and its applications, belonging to the fields of plant molecular biotechnology and genetic engineering. This gene is derived from *Prunus glabra*, with a full-length CDS sequence of 378 bp. The encoded protein contains a conserved SBP domain, and its nucleotide sequence is shown in SEQ ID NO.1. It was cloned from the bracts of the 'Snow White' variety at the BR3 stage. Bioinformatics analysis, subcellular localization, virus-induced gene silencing, and heterologous overexpression experiments verified that the protein encoded by this gene is located in the cell nucleus and cell membrane. Silencing this gene leads to bract and stem / leaf abortion in *Prunus glabra*, while overexpression promotes growth and earlier flowering in *Arabidopsis thaliana*. This application identifies a key gene for *Prunus glabra* bract development, which can be used to promote plant growth and flowering, and has important application value in the molecular breeding of ornamental plants.
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