A method for cultivating sower of tiger lily

Abstract

The application discloses a method for cultivating Tremellodon sphagni, and belongs to the technical field of edible mushroom cultivation, in particular relates to a method for cultivating Tremellodon sphagni, which comprises the following steps: preparing Tremellodon sphagni sticks, disinfecting a planting site, ditching and ridging, planting the prepared Tremellodon sphagni sticks in the ditch, covering soil, watering, and culturing for 38 days to obtain Tremellodon sphagni; the Tremellodon sphagni stick is prepared from the following raw materials in parts by weight: corn cob 710-730 parts, sawdust 1140-1160 parts, bran 495-505 parts, corn flour 218-222 parts, beet residue 215-225 parts, lime 38-42 parts, light calcium carbonate 40 parts and Tremellodon sphagni mycelium 0.1-1 part. The Tremellodon sphagni is cultivated by using the Tremellodon sphagni stick, so that the yield and quality of the cultivated Tremellodon sphagni are improved, and the Tremellodon sphagni is not prone to diseases and insect pests during the cultivation process.

Description

Technical Field

[0001] This invention belongs to the field of edible fungi cultivation technology, and in particular relates to a method for cultivating tiger paw fungus. Background Technology

[0002] Tiger paw mushroom is a rare and precious wild edible fungus. It gets its name from the fine, yellowish-brown hairs covering its body, which are adorned with distinct black markings resembling a tiger's paw. Rich in nutrients, tiger paw mushrooms are delicious, tender, and have a unique aroma. Fresh tiger paw mushrooms have a strong fragrance, which intensifies after drying. Known for their unusual shape and unique aroma, they were historically considered a precious delicacy and were among the tributes offered to successive dynasties. Currently, they are one of my country's top ten famous mushrooms. The tiger paw mushroom has a robust and large body, rich in extracellular polysaccharides, making it resistant to spoilage and highly nutritious. Analysis shows that the dried product contains 17 amino acids, including 7 essential amino acids (41.46% of the total), as well as 11 minerals and trace elements. Tiger paw mushrooms are neutral in nature and sweet in taste, possessing properties that dispel wind and cold, promote blood circulation, clear heat, and reduce inflammation. They can lower cholesterol, promote metabolism, and enhance immunity, and have certain therapeutic effects on gastric ulcers, chronic gastritis, and stomach pain. Tiger paw mushroom is not only a traditional high-end edible mushroom variety in my country, but it is also regarded as a top-quality edible mushroom in Japan and Southeast Asia. It has a large market demand, extremely high economic value, and a very expensive market price.

[0003] However, tiger paw mushrooms have long relied primarily on wild collection, resulting in extremely low yields, difficult collection, and increasingly scarce resources. Wild tiger paw mushrooms mostly grow in coniferous and broad-leaved forests such as Yunnan pine, fir, and alpine oak at altitudes above 1500 meters. They only grow in the depths of grasslands and coniferous forests on high-altitude cliffs from August to September each year, with a very limited distribution range. Affected by climate and environmental factors, the yield of wild gray tiger paw mushrooms is extremely limited. Currently, the main means of protecting tiger paw mushroom resources is artificial domestication, but despite years of effort to achieve artificial cultivation, no breakthrough has been made for a long time.

[0004] The fundamental reason for the difficulty in artificially cultivating Tiger Paw Fungus lies in the fact that it differs from common wood-rotting edible fungi. As an ectomycorrhizal fungus, it must rely on tree roots for symbiosis to grow and develop in the wild, making artificial cultivation challenging. Currently, artificial cultivation is still in the early stages of research and trial planting. Although some progress has been made, yields are unstable, the fruiting rate is low, and large-scale, standardized production is difficult to achieve. For example, the article "Simulated Ecological Cultivation Technology of Tiger Paw Fungus in Forest Land" published in the 5th issue of *Shandong Forestry Science and Technology* in 2014 clearly points out that artificial cultivation of Tiger Paw Fungus is difficult and has a low success rate.

[0005] In existing technologies, some studies have attempted to cultivate *Tiger Paw Fungus* artificially using mushroom bag cultivation methods. For example, patent publication number CN110447461A discloses a cultivation method to improve the yield and quality of edible fungi, including steps such as mushroom bag preparation, edible fungi inoculation, and cultivation management. By using sesame straw to prepare cultivation bags, it achieves the effect of improving the yield and quality of edible fungi without the use of any pesticides, with a yield of up to 610 kg / mu, a first-grade product rate of up to 98%, and a disease and pest incidence rate as low as 2%. However, this technical solution is aimed at conventional wood-rotting edible fungi and does not address the special nutritional needs of *Tiger Paw Fungus* as an ectomycorrhizal fungus. Another example is patent publication number CN111771609A, which discloses a method for cultivating *Red-topped Bamboo Fungus* under forest cover using mushroom bags. This method improves the utilization rate of forest land resources, reduces cultivation and management costs, has a shorter fruiting period, and produces *Red-topped Bamboo Fungus* of higher quality through a semi-wild cultivation model under forest cover. However, this technology is mainly designed for the specific variety of *Dictyophora indicum*, and its culture medium formulation and cultivation management model are not suitable for the special growth requirements of *Tiger Paw Fungus*.

[0006] In general, existing artificial cultivation techniques for tiger paw mushrooms have the following shortcomings: First, the yield is unstable and the fruiting rate is low. Second, the incidence of pests and diseases is relatively high. Third, the cultivation cycle is long and the utilization rate of land resources is low.

[0007] Therefore, developing a high-yield, high-quality, and pest-resistant cultivation method for tiger paw mushrooms, and realizing large-scale, standardized, and year-round production of tiger paw mushrooms, is of great significance for alleviating the endangered pressure of wild tiger paw mushroom resources, meeting the growing market demand, and promoting the high-quality development of the edible fungi industry. Summary of the Invention

[0008] To address the aforementioned technical problems, this invention proposes a method for cultivating Tiger Paw Fungus. This invention uses Tiger Paw Fungus spawn for cultivation, which improves the yield and quality of the cultivated Tiger Paw Fungus and reduces the likelihood of pests and diseases during the cultivation process.

[0009] To achieve the above objectives, the present invention provides a method for cultivating *Tectus tigrinosa*, comprising the following steps: preparing *Tectus tigrinosa* spawn, disinfecting the planting site, digging trenches and creating ridges, planting the prepared *Tectus tigrinosa* spawn in the trenches, covering with soil, watering, and cultivating for 38 days to obtain *Tectus tigrinosa*; the *Tectus tigrinosa* spawn is prepared from the following raw materials in parts by weight: 710-730 parts corn cob, 1140-1160 parts sawdust, 495-505 parts wheat bran, 218-222 parts corn flour, 215-225 parts beet pulp, 38-42 parts lime, 40 parts light calcium carbonate, and 0.1-1 parts *Tectus tigrinosa* mycelium.

[0010] Preferably, the length of the tiger paw fungus stick is 16-20cm.

[0011] Preferably, the Tiger Paw Fungus spawn is made by mixing corn cobs, sawdust, wheat bran, corn flour, beet pulp, lime, and light calcium carbonate, inoculating Tiger Paw Fungus mycelium, culturing, post-ripening for 30 days, inducing bud formation, and then cultivating mushrooms.

[0012] More preferably, the culture is carried out in the dark, the culture temperature is 20~25℃, the culture humidity is 65%~70%, and the culture time is 7~11 days.

[0013] More preferably, the temperature for inducing bud formation is 12~18℃, the light intensity for inducing bud formation is 100~200Lx, the humidity for inducing bud formation is 85%, the CO2 concentration for inducing bud formation is <1000ppm, and the bud formation time is 3~7 days.

[0014] More preferably, the temperature for mushroom cultivation is 13~16℃, the light intensity for mushroom cultivation is 200~500Lx, the humidity for mushroom cultivation is 85%~90%, the CO2 concentration for mushroom cultivation is <2000ppm, and the cultivation time is 7~15 days.

[0015] Preferably, the planting site is sandy soil, and the planting site is disinfected by spreading lime, then tilling the soil to fully mix the lime with the topsoil to a depth of 15-20cm. The furrows and ridges are dug with a ridge height of 10-20cm and a ridge width of 60-80cm.

[0016] Preferably, the spacing between the *Tiger Paw* mycelium sticks is 15-25 cm.

[0017] Preferably, the thickness of the soil covering is 1-3 cm, and the watering is carried out until the soil moisture content is 70%-90%.

[0018] Preferably, the culture temperature is 10~18℃.

[0019] Compared with the prior art, the present invention has the following advantages and technical effects: This invention provides a method for cultivating *Tiger Paw* fungus. The method uses *Tiger Paw* spawn logs for cultivation, improving the yield and quality of the cultivated fungus and reducing the likelihood of pests and diseases during cultivation. The spawn logs of this invention primarily use corn cobs and sawdust, supplemented with wheat bran, corn flour, beet pulp, lime, and light calcium carbonate. Compared to the commonly used "cottonseed hulls and soybean meal" formula in existing technologies, the beet pulp and other components in the raw materials of this invention provide a more balanced carbon-nitrogen ratio and trace elements for *Tiger Paw* growth. The spawn logs using this formula exhibit vigorous mycelial growth, rapid colonization, uniform fruiting, and significantly higher biological efficiency than those using cottonseed hulls and soybean meal. This cultivation method enables large-scale, standardized production of *Tiger Paw* fungus, with high fruiting rate and stable yield, achieving the goal of high production. This invention simulates the natural growth environment of *Tectus tigrinosa*, resulting in fruiting bodies with intact morphology, firm texture, and rich aroma. It ensures each mushroom log has ample growth space and nutrient supply, producing uniformly sized mushrooms with excellent marketability and a high rate of first-grade products. This invention effectively kills pathogens and insect eggs in the soil. The addition of lime to the mushroom log formula inhibits the growth of unwanted microorganisms, and the addition of light calcium carbonate buffers pH fluctuations, maintaining a stable microenvironment. The low-temperature cultivation conditions employed are unfavorable to the growth of most unwanted microorganisms and pests, thus significantly reducing the incidence of diseases and pests. No chemical pesticides are required, making the product green and safe.

[0020] In summary, this invention, through a combination of a special mushroom substrate formula and a fully optimized cultivation process, achieves high-yield, high-quality, and disease-resistant cultivation of Tiger Paw Fungus, overcoming the technical difficulties of low yield, poor quality, and susceptibility to disease in existing artificial cultivation techniques for Tiger Paw Fungus, and has good prospects for industrial application. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 The tiger paw fungus spawn prepared in Example 1 of this invention; Figure 2 The tiger paw fungus cultivated in Example 1 of this invention; Figure 3 This is a diagram of the cultivation of Tiger Paw Fungus spawn in Embodiment 1 of the present invention; Figure 4 This is a cultivation map of Tiger Paw Fungus grown in Example 1 of the present invention. Detailed Implementation

[0023] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0024] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0025] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0026] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0027] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0028] Unless otherwise specified, the term "parts" in this invention refers to parts by weight.

[0029] Example 1 Preparation of Tiger Paw Fungus spawn: 720 parts corn cob, 220 parts beet pulp, and 1150 parts sawdust are added in sequence and stirred for 10 minutes. Then, 500 parts wheat bran, 220 parts corn flour, 40 parts lime, and 40 parts light calcium carbonate are added and stirred for 10 minutes. Water is then added and stirred for 30 minutes. The mixture is then bagged, with each bag weighing 1700g. The humidity is maintained within the range of 69% to 70%, and the pH is maintained within the range of 10.0 to 11.5. The total amount of water added is 800 parts. After bagging, autoclave at 121-123℃ for 90 minutes. After cooling, inoculate with 0.5 portions of *Tiger Paw* mycelium and culture in the dark at 20-25℃ and 65%-70% humidity for 9 days, followed by 30 days of post-ripening. Then, induce fruiting for 5 days at 12-18℃, 100-200 Lx light intensity, 85% humidity, and <1000 ppm CO2 concentration. Finally, cultivate mushrooms for 11 days at 200-500 Lx light intensity, 85%-90% humidity, and <2000 ppm CO2 concentration. Harvest *Tiger Paw* mycelium logs with a length of 20cm (e.g., ...). Figure 1 (As shown).

[0030] Disinfect the sandy soil by applying lime, then till the soil, thoroughly mixing the lime with the topsoil to a depth of 15-20cm. Create furrows and ridges, 15cm high and 70cm wide. Plant the prepared *Tiger Paw* spawn in the furrows at a spacing of 20cm. Figure 3 (As shown), cover with 2cm of soil, water until the soil moisture content is 80%, and culture in an environment with a temperature of 10~18℃ for 38 days to obtain Tiger Paw Fungus (as shown). Figure 2 and Figure 4 (As shown).

[0031] Example 2 Preparation of Tiger Paw Fungus spawn: 710 parts corn cob, 215 parts beet pulp, and 1140 parts sawdust are added sequentially and stirred for 10 minutes. Then, 495 parts wheat bran, 218 parts corn flour, 38 parts lime, and 40 parts light calcium carbonate are added and stirred for 10 minutes. Water is then added and stirred for 30 minutes. The mixture is then bagged, with each bag weighing 1650g. The humidity is maintained within the range of 69% to 70%, and the pH is maintained within the range of 10.0 to 11.5. The total amount of water added is 750 parts. After bagging, autoclave at 121-123℃ for 60 minutes. After cooling, inoculate with 0.1 part of Tiger Paw Fungus mycelium and culture in the dark for 7 days at 20-25℃ and 65%-70% humidity. After 30 days of post-ripening, induce fruiting for 3 days at 12-18℃, 100-200 Lx light intensity, 85% humidity and <1000 ppm CO2 concentration. Then, cultivate mushrooms for 15 days at 200-500 Lx light intensity, 85%-90% humidity and <2000 ppm CO2 concentration. Harvest Tiger Paw Fungus logs with a length of 18 cm after thinning the fruiting.

[0032] Disinfect the sandy soil by spreading lime, then till the soil, mixing the lime thoroughly with the topsoil to a depth of 15-20cm. Make furrows and ridges, 10cm high and 60cm wide. Plant the prepared tiger paw fungus spawn in the furrows at a spacing of 15cm, cover with 1cm of soil, and water until the soil moisture content is 70%. Cultivate in an environment with a temperature of 10-18℃ for 38 days to obtain tiger paw fungus.

[0033] Example 3 Preparation of Tiger Paw Fungus spawn: 730 parts corn cob, 225 parts beet pulp, and 1160 parts sawdust were added in sequence and stirred for 10 minutes. Then, 505 parts wheat bran, 222 parts corn flour, 42 parts lime, and 40 parts light calcium carbonate were added and stirred for 10 minutes. Water was added and stirred for 30 minutes. The mixture was then bagged, with each bag weighing 1750g. The humidity was maintained within the range of 69% to 70%, and the pH was maintained within the range of 10.0 to 11.5. The total amount of water added was 850 parts. After bagging, autoclave at 121-123℃ for 120 minutes. After cooling, inoculate with one portion of Tiger Paw Fungus mycelium and culture in the dark for 11 days at 20-25℃ and 65%-70% humidity. After 30 days of post-ripening, induce fruiting for 7 days at 12-18℃, 100-200 Lx light intensity, 85% humidity, and <1000 ppm CO2 concentration. Then, cultivate mushrooms for 15 days at 200-500 Lx light intensity, 85%-90% humidity, and <2000 ppm CO2 concentration. Harvest Tiger Paw Fungus logs with a length of 16 cm after thinning the fruiting.

[0034] Disinfect the sandy soil by spreading lime, then till the soil, mixing the lime thoroughly with the topsoil to a depth of 15-20cm. Make furrows and ridges, 20cm high and 80cm wide. Plant the prepared tiger paw fungus spawn in the furrows at a spacing of 25cm, cover with 3cm of soil, and water until the soil moisture content is 90%. Cultivate in an environment with a temperature of 10-18℃ for 38 days to obtain tiger paw fungus.

[0035] Comparative Example 1 Preparation of Tiger Paw Fungus spawn: 720 parts cottonseed hulls, 220 parts soybean meal, and 1150 parts sawdust were added in sequence and stirred for 10 minutes. Then, 500 parts wheat bran, 220 parts corn flour, 40 parts lime, and 40 parts light calcium carbonate were added and stirred for 10 minutes. Water was added and stirred for 30 minutes. The mixture was then bagged, with each bag weighing 1700g. The humidity was maintained within the range of 69% to 70%, and the pH was maintained within the range of 10.0 to 11.5. The total amount of water added was 800 parts. After bagging, autoclave at 121-123℃ for 90 minutes. After cooling, inoculate with 0.5 portions of Tiger Paw Fungus mycelium and culture in the dark for 9 days at 20-25℃ and 65%-70% humidity. After post-ripening for 30 days, induce fruiting for 5 days at 12-18℃, 100-200 Lx light intensity, 85% humidity, and <1000 ppm CO2 concentration. Then, cultivate mushrooms for 11 days at 200-500 Lx light intensity, 85%-90% humidity, and <2000 ppm CO2 concentration. Harvest Tiger Paw Fungus logs with a length of 20 cm after thinning the fruiting.

[0036] Disinfect the sandy soil by spreading lime, then till the soil, mixing the lime thoroughly with the topsoil to a depth of 15-20cm. Make furrows and ridges, 15cm high and 70cm wide. Plant the prepared tiger paw fungus spawn in the furrows at a spacing of 20cm, cover with 2cm of soil, and water until the soil moisture content is 80%. Cultivate in an environment with a temperature of 10-18℃ for 38 days to obtain tiger paw fungus.

[0037] Comparative Example 2 Preparation of Tiger Paw Fungus spawn: 720 parts corn cob, 220 parts beet pulp, and 1150 parts sawdust are added in sequence and stirred for 10 minutes. Then, 500 parts wheat bran, 220 parts corn flour, 40 parts lime, and 40 parts light calcium carbonate are added and stirred for 10 minutes. Water is then added and stirred for 30 minutes. The mixture is then bagged, with each bag weighing 1700g. The humidity is maintained within the range of 69% to 70%, and the pH is maintained within the range of 10.0 to 11.5. The total amount of water added is 800 parts. After bagging, autoclave at 121-123℃ for 90 minutes. After cooling, inoculate with 0.5 portions of Tiger Paw Fungus mycelium and culture in the dark at 25-28℃ and 75% humidity for 9 days. After post-ripening for 30 days, induce fruiting for 5 days at 19-22℃, light intensity of 300-350 Lx, humidity of 90%, and CO2 concentration of <1000 ppm. Then, cultivate mushrooms for 11 days at light intensity of 600-700 Lx, humidity of 95%, and CO2 concentration of <2000 ppm. Harvest Tiger Paw Fungus logs with a length of 20 cm after thinning the fruiting.

[0038] Disinfect the sandy soil by spreading lime, then till the soil, mixing the lime thoroughly with the topsoil to a depth of 15-20cm. Make furrows and ridges, 15cm high and 70cm wide. Plant the prepared tiger paw fungus spawn in the furrows at a spacing of 20cm, cover with 2cm of soil, and water until the soil moisture content is 60%. Cultivate in an environment with a temperature of 20℃ for 38 days to obtain tiger paw fungus.

[0039] Example 4 Tiger paw fungus was cultivated in greenhouses using the cultivation methods described in Example 1, Comparative Example 1, and Comparative Example 2, respectively. The yield per acre for each cultivation method was calculated. The nutritional components of the tiger paw fungus cultivated using each method were determined. Moisture content was determined according to Method 1 of GB5009.3—2016 "Determination of Moisture in Food"; protein content was determined according to the Kjeldahl method of GB5009.5—2016 "Determination of Protein in Food"; fat content was determined according to GB5009.6—2016 "Determination of Fat in Food"; crude fiber content was determined according to GB / T 5009.10—2016 "Determination of Crude Fiber in Plant-Based Foods"; and ash content was determined according to GB5009.4—2016 "Determination of Ash Content in Food".

[0040] Table 1. Yield per mu (unit of land area) of cultivation methods in Example 1 and Comparative Examples 1-2

[0041] Table 2. Nutritional composition of *Tectus tigrinosa* cultivated using the methods of Example 1 and Comparative Examples 1-2

[0042] As can be seen from Tables 1 and 2, the cultivation method described in Example 1 of this invention significantly increases the yield of Tiger Paw Fungus per acre, and the prepared Tiger Paw Fungus is rich in protein, fat and crude fiber, thus improving the quality of Tiger Paw Fungus.

[0043] Example 5 By comparing the incidence and disease index of fungal diseases under different cultivation methods in Example 1 and Comparative Examples 1-2, cultivation methods with better disease resistance were selected.

[0044] Experimental location: Standardized, controlled-environment greenhouse to reduce interference from environmental variables.

[0045] Experimental Design: Three groups were set up, including Example 1 and Comparative Examples 1-2, using different cultivation methods, with each group replicated at least three times. The greenhouse was divided into independent plots of equal area. Each cultivation method was assigned one plot. The plots were arranged randomly to reduce the influence of minor environmental differences within the greenhouse (such as light and ventilation) on the experimental results. Three replicates were used to help assess the stability and reliability of the results and avoid random errors.

[0046] Record and differentiate the symptoms of fungal diseases, bacterial diseases, and insect pests in detail.

[0047] Disease classification and standards: Incidence rate (%) = (Number of diseased specimens / Total number of specimens surveyed) × 100%.

[0048] Disease Index (DI): This is a comprehensive indicator of disease severity. First, the disease severity of individual mushroom logs is classified into different levels, such as: Level 0 (no lesions), Level 1 (lesion area ≤ 25%), Level 2 (25% < lesion area ≤ 50%), Level 3 (50% < lesion area ≤ 75%), and Level 4 (lesion area > 75%). Then, the following formula is used for calculation: Disease index = [∑(number of diseased plants at each level × representative value of that level) / (total number of plants surveyed × highest level representative value)] × 100%.

[0049] Table 3 Disease resistance of cultivation methods in Example 1 and Comparative Examples 1-2

[0050] As shown in Table 3, the cultivation method described in Example 1 has a low incidence rate and a low disease index, which can ensure the normal and stable production of Tiger Paw Fungus. In contrast, Comparative Example 2, due to improper environmental conditions such as temperature and humidity during cultivation, significantly increased the incidence rate and disease index.

[0051] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A method for cultivating tiger paw fungus, characterized in that, Includes the following steps: Prepare tiger paw mushroom spawn, disinfect the planting site, dig trenches and ridges, plant the prepared tiger paw mushroom spawn in the trenches, cover with soil, water, and cultivate for 38 days to obtain tiger paw mushroom. The Tiger Paw Fungus spawn is prepared from the following raw materials in parts by weight: 710-730 parts corn cob, 1140-1160 parts sawdust, 495-505 parts wheat bran, 218-222 parts corn flour, 215-225 parts beet pulp, 38-42 parts lime, 40 parts light calcium carbonate, and 0.1-1 parts Tiger Paw Fungus mycelium.

2. The method for cultivating Tiger Paw Fungus according to claim 1, characterized in that, The length of the tiger paw fungus log is 16-20cm.

3. The method for cultivating Tiger Paw Fungus according to claim 1, characterized in that, The Tiger Paw Fungus spawn is made by mixing corn cobs, sawdust, wheat bran, corn flour, beet pulp, lime, and light calcium carbonate, inoculating Tiger Paw Fungus mycelium, culturing, post-ripening for 30 days, inducing bud formation, and then cultivating mushrooms.

4. The method for cultivating Tiger Paw Fungus according to claim 3, characterized in that, The culture is carried out in the dark, the culture temperature is 20~25℃, the culture humidity is 65%~70%, and the culture time is 7~11 days.

5. The method for cultivating Tiger Paw Fungus according to claim 3, characterized in that, The temperature for inducing bud formation is 12~18℃, the light intensity for inducing bud formation is 100~200Lx, the humidity for inducing bud formation is 85%, the CO2 concentration for inducing bud formation is <1000ppm, and the bud formation time is 3~7 days.

6. The method for cultivating Tiger Paw Fungus according to claim 3, characterized in that, The temperature for mushroom cultivation is 13~16℃, the light intensity for mushroom cultivation is 200~500Lx, the humidity for mushroom cultivation is 85%~90%, the CO2 concentration for mushroom cultivation is <2000ppm, and the cultivation time is 7~15 days.

7. The method for cultivating Tiger Paw Fungus according to claim 1, characterized in that, The planting site is sandy soil. The disinfection of the planting site is carried out by spreading lime, followed by tilling, and mixing the lime with the topsoil to a depth of 15-20cm. The furrows are dug and ridges are made, with the ridges being 10-20cm high and 60-80cm wide.

8. The method for cultivating Tiger Paw Fungus according to claim 1, characterized in that, The spacing between the plantlets of the Tiger Paw Fungus is 15-25cm.

9. The method for cultivating Tiger Paw Fungus according to claim 1, characterized in that, The thickness of the soil covering is 1-3 cm, and the watering is carried out until the soil moisture content is 70%-90%.

10. The method for cultivating Tiger Paw Fungus according to claim 1, characterized in that, The culture temperature is 10~18℃.

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