A fat composition and uses thereof

Abstract

The present application provides a kind of oil composition, containing monounsaturated fatty acid, polyunsaturated fatty acid, monounsaturated fatty acid is omega-9 fatty acid;Polyunsaturated acid is the mixture of omega-6 fatty acid and omega-3 fatty acid.The present application can repair and resist aging, all-purpose skin care, increase epidermal thickness, enhance barrier, anti-inflammatory soothing, anti-wrinkle firming, soothing repair red, nourish skin, and can be applied to sensitive skin, can inhibit LPS-induced HaCaT cell IL-6 secretion, increase the thickness of 3D skin model epidermis layer, and can promote the expression of silk aggregate protein (FLG), ceramide synthase 3 (CerS3), desmoglein core glycoprotein (DSG1) which has important role for skin barrier function, indicating that it can alleviate the inflammatory response produced by LPS stimulation HaCaT cell and has enhanced skin barrier function.

Description

Technical Field

[0001] This invention belongs to the field of compositions, and more specifically relates to the field of cosmetics, skin care products or pharmaceutical preparations, particularly to a composition for improving skin condition, specifically a composition for increasing epidermal thickness, anti-wrinkle and firming, and anti-inflammatory and soothing. Background Technology

[0002] With the accelerated pace of modern life and increased environmental pollution, skin aging is becoming increasingly prevalent and affecting younger people. According to the World Association for Skin Health, approximately 83% of adults worldwide show early signs of aging before the age of 30, with over 40% of them having sensitive skin. This has driven the anti-aging skincare market to grow at an average annual rate of 7.5%. However, current technology still faces three major bottlenecks: First, traditional anti-aging ingredients such as retinol and vitamin A derivatives are highly irritating, resulting in poor suitability for sensitive skin; second, single-function ingredients cannot meet the complex needs of "repair, anti-aging, and skin stabilization"; and third, long-term use of synthetic chemical ingredients may damage the skin barrier.

[0003] In the application of natural plant extracts, current technologies mostly focus on the development of single oil-phase or water-phase active ingredients. Single natural oils are often high in one or two types of unsaturated fatty acids, and these fatty acids are often unstable. No single natural oil simultaneously contains a balanced amount of monounsaturated fatty acids (ω-9) and polyunsaturated fatty acids (ω-6 and ω-3) in a stable state. ω-6 and ω-3 are essential fatty acids, indispensable for maintaining bodily functions, but cannot be synthesized by the body and must be obtained through supplementation. They are important components of lipids in biological membranes and are significant for maintaining normal skin function; furthermore, nutritional studies have shown that plant oils with a balanced ratio of ω-9, ω-6, and ω-3 have better physiological activity. Furthermore, existing formulations generally lack mechanisms for regulating the skin's microecology, which are crucial for the specific needs of sensitive skin.

[0004] In terms of efficacy verification, existing evaluation systems have limitations: in vitro antioxidant tests (such as the DPPH method) are difficult to reflect actual skincare effects; animal alternative tests (such as reconstructed human epidermal models) cannot simulate the special stress responses of sensitive skin. This leads to many products claiming to be "gentle anti-aging" failing to achieve both efficacy and safety in actual use. According to statistics from the EU's Cosmetic Adverse Reaction Monitoring System, 46.7% of complaints about anti-aging products in 2022 involved irritation reactions.

[0005] At the formulation technology level, existing technologies use microencapsulation to improve ingredient stability, but when the encapsulation rate exceeds 80%, it leads to poor sustained-release effects of active ingredients; insufficient particle size control in nanoemulsification processes results in large fluctuations in transdermal efficiency. These technological shortcomings severely restrict the development of multi-functional natural skincare products.

[0006] Therefore, there is an urgent need to develop a new type of compound plant active ingredient system that, while ensuring gentleness, achieves multiple effects such as anti-wrinkle and firming, barrier repair, and microecological balance through multi-pathway synergistic action, especially to meet the special skin care needs of people with sensitive skin. Summary of the Invention

[0007] In view of the above-mentioned deficiencies in the prior art, the present invention provides a skin care oil composition and its use, more specifically relating to a composition and its use in cosmetics, skin care products, or pharmaceutical preparations. The specific technical solution adopted by the present invention is as follows: In a first aspect of the present invention, an oil composition is provided, characterized in that it contains monounsaturated fatty acids and polyunsaturated fatty acids.

[0008] Monounsaturated fatty acids are a class of fatty acids with one double bond, commonly found in many natural foods, with oleic acid being the most typical example. Polyunsaturated fatty acids are a class of fatty acids containing two or more double bonds, mainly divided into ω-3 and ω-6 series based on the position of the double bonds. They cannot be synthesized into essential fatty acids in the human body and must be obtained through diet. They are important components of lipids in biological membranes, and are particularly significant in maintaining normal skin function.

[0009] In some specific embodiments, the monounsaturated fatty acid is an ω-9 fatty acid; the polyunsaturated acid is a mixture of ω-6 and ω-3 fatty acids. Examples of suitable monounsaturated fatty acids include, but are not limited to, ω-9 fatty acids. Examples of suitable polyunsaturated acids include, but are not limited to, mixtures of ω-6 and ω-3 fatty acids.

[0010] In some specific embodiments, the ω-6 fatty acid is selected from one or more of linoleic acid, gamma-linolenic acid, arachidonic acid, and conjugated linoleic acid; the ω-3 fatty acid is selected from one or more of α-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid, and docosahexaenoic acid; and the ω-9 fatty acid is selected from one or more of oleic acid, palmitoleic acid, erucic acid, codic acid, and cis-15-docosahexaenoic acid. Examples of suitable ω-6 fatty acids include, but are not limited to, linoleic acid, gamma-linolenic acid, arachidonic acid, and conjugated linoleic acid. Examples of suitable ω-3 fatty acids include, but are not limited to, α-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid, and docosahexaenoic acid. Examples of suitable ω-9 fatty acids include, but are not limited to, oleic acid, palmitoleic acid, erucic acid, codic acid, and cis-15-docosahexaenoic acid.

[0011] In some specific implementations, the weight ratio of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid is 0.3~4:0.3~4:1.

[0012] Furthermore, the weight ratio of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid is 1~4:1~4:1.

[0013] Furthermore, the weight ratio of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid is 1~3:1~3:1.

[0014] Furthermore, the weight ratio of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid is 1.5~3:1.5~3:1.

[0015] Furthermore, the weight ratio of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid is 2~3:2~3:1.

[0016] In a preferred embodiment, the weight ratio of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid is 27~33:27~33:17~23; more specifically, it is 28~32:28~32:18~22.

[0017] In some specific embodiments, the total weight of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid accounts for more than 60% of the total weight of the oil mixture; preferably more than 80%.

[0018] Preferably, in some specific embodiments, the ω-9 fatty acid accounts for more than 20% of the total weight of the oil mixture, the ω-6 fatty acid accounts for more than 20% of the total weight of the oil mixture, and the ω-3 fatty acid accounts for more than 5% of the total weight of the oil mixture.

[0019] More preferably, in some specific embodiments, the ω-9 fatty acid accounts for more than 25% of the total weight of the oil mixture, the ω-6 fatty acid accounts for more than 25% of the total weight of the oil mixture, and the ω-3 fatty acid accounts for more than 15% of the total weight of the oil mixture.

[0020] In some specific embodiments, the composition comprises perilla seed oil, rice bran oil, red pine seed oil, and patchouli oil. Further, the composition also contains rosemary leaf extract.

[0021] Furthermore, the composition contains the following components in weight percentage: 10%~50% perilla seed oil, 30%~80% rice bran oil, 2%~30% red pine seed oil, and 0.1%~10% patchouli oil.

[0022] In some specific embodiments, the composition further contains 0.005% to 1% rosemary leaf extract.

[0023] In some specific embodiments, the composition contains the following components in weight percentages: 20%~40% perilla seed oil, 40%~75% rice bran oil, 3%~20% red pine seed oil, and 0.3%~8% patchouli oil.

[0024] In some specific embodiments, the composition further contains 0.01% to 0.5% rosemary leaf extract.

[0025] In some specific embodiments, the composition contains the following components in weight percentages: 20%~40% perilla seed oil, 40%~75% rice bran oil, 3%~20% red pine seed oil, 0.3%~5% patchouli oil, and 0.01%~0.5% rosemary leaf extract.

[0026] In some specific embodiments, the composition contains the following components in weight percentages: 25%~40% perilla seed oil, 40%~60% rice bran oil, 8%~18% red pine seed oil, 0.5%~2% patchouli oil, and 0.01%~0.2% rosemary leaf extract.

[0027] The aforementioned composition may be used in the preparation of cosmetics, skin care products, or pharmaceutical preparations that increase epidermal thickness, reduce wrinkles and firm, and have anti-inflammatory and soothing effects.

[0028] In a second aspect of the invention, the oil composition is used in the preparation of cosmetics, skin care products, or pharmaceutical preparations that increase epidermal thickness, reduce wrinkles and firm, and have anti-inflammatory and soothing effects.

[0029] In a third aspect of the invention, a personal care composition is also provided, which contains 0.01 to 20% by weight of the aforementioned oil composition based on the total weight of the personal care composition, and is capable of achieving the effects of increasing epidermal thickness, anti-wrinkle and firming, and anti-inflammatory and soothing.

[0030] Compared with the prior art, the present invention has the following beneficial effects: This invention repairs and combats aging, provides all-around skin care, increases epidermal thickness, strengthens the skin barrier, has anti-inflammatory and soothing properties, reduces wrinkles and firms the skin, soothes redness, and nourishes and softens the skin. It is suitable for sensitive skin and can inhibit LPS (Lipopolysaccharide)-induced IL-6 secretion in HaCaT cells, increase the thickness of the epidermal layer in a 3D skin model, and promote the expression of filaggrin (FLG), ceramide synthase 3 (CerS3), and desmosome core glycoprotein (DSG1), which play important roles in skin barrier function. This indicates that it can alleviate the inflammatory response of HaCaT cells stimulated by LPS and enhance skin barrier function. The composition provided by this invention is a novel complex system of plant-based active ingredients.

[0031] The following will further explain the concept and technical effects of the present invention in conjunction with the accompanying drawings, so as to fully understand the purpose, features and effects of the present invention. Attached Figure Description

[0032] Figure 1 The sample affects the secretion of the inflammatory factor IL-6 in HaCaT cells.

[0033] Figure 2 This is a schematic diagram (40X) showing the effect of the sample on the tissue morphology of the 3D epidermal model.

[0034] Figure 3 It is the effect of the sample on the tissue morphology of the 3D epidermal model.

[0035] Figure 4 This is an example diagram (40X) showing the effect of the sample on the relative fluorescence intensity of FLG protein in a 3D epidermal model.

[0036] Figure 5 This study analyzes the influence of the sample on the relative fluorescence intensity of FLG protein in a 3D epidermal model.

[0037] Figure 6 This is an example image (40X) showing the effect of the sample on the relative fluorescence intensity of the 3D epidermal model CerS3.

[0038] Figure 7 This study analyzes the influence of the sample on the relative fluorescence intensity of the 3D epidermal model CerS3.

[0039] Figure 8 Example image (40X) showing the effect of the sample on the relative fluorescence intensity of DSG1 protein in a 3D epidermal model.

[0040] Figure 9 Analysis of the effect of samples on the relative fluorescence intensity of DSG1 protein in a 3D epidermal model.

[0041] Figure 10 Analysis of the effect of samples on the relative fluorescence intensity of DSG1 protein in a 3D epidermal model. Detailed Implementation

[0042] To make the technical means, inventive features, objectives, and effects of the invention readily understandable, the invention is further illustrated below with reference to specific figures. However, the invention is not limited to the embodiments described below.

[0043] It should be noted that the accompanying drawings are only used to complement the content disclosed in this specification, so as to enable those skilled in the art to understand and read them, and are not intended to limit the conditions under which the present invention can be implemented. Therefore, they have no substantial technical significance. Any non-creative changes, without affecting the effects and objectives that the present invention can produce, should still fall within the scope of the technical content disclosed in the present invention.

[0044] The reagents used in the following examples are as follows: Table 1 Reagent Information The above composition is referred to as Golden Purple Rice Oil in the following examples.

[0045] Example 1: Detection of inflammatory cytokine (IL-6) levels in HaCaT cells Detection method: The ELISA assay was used to quantitatively assess the effect of the sample on the release of inflammatory factors, thereby evaluating the efficacy of the sample at the cellular level.

[0046] Detection indicators: Calculation of the relative content (%) of relevant cytokines: The change in the concentration of relevant cytokines after sample addition was measured by comparing with the control group.

[0047] Group settings The ingredients of Golden Purple Rice Oil are: 33.9% perilla seed oil, 50% rice bran oil, 15% red pine seed oil, 1% patchouli oil, and 0.1% rosemary leaf extract.

[0048] Detection steps Cell seeding: Take cells in the logarithmic growth phase, use DMEM medium containing 10% FBS, and when the cell density is about 80%, digest with 0.05% trypsin, centrifuge at 1000 rpm for 5 min, resuspend and count, seed in 24-well plates, and culture at 37℃ and 5% CO2.

[0049] Sample preparation: After 24 hours of inoculation, the culture medium was aspirated. The blank group was treated with complete culture medium, the control group was treated alone with complete culture medium containing 10 μg / mL LPS, and the sample group was treated with 10 μg / mL LPS and the corresponding concentration of sample. The samples were incubated for another 24 hours. After exposure, the supernatant was collected, centrifuged, and the secretion of IL-6 inflammatory factor was detected using an ELISA kit.

[0050] Data analysis: Calculate the concentrations of relevant cytokines before and after sample addition based on the standard curve; Relative content (%) = Image processing was performed using Graphpad Prism graphics software, and the results are expressed as Mean ± SD. A t-test was used to compare the results with the control group. *P < 0.05 indicates a statistically significant difference, **P < 0.01 indicates a statistically significant difference, and ***P < 0.001 indicates an extremely statistically significant difference. Judgment criteria: If the relative content (%) of IL-6 is <100% compared with the control group, then the sample at this concentration is judged to be able to inhibit the secretion of the inflammatory factor IL-6.

[0051] Experimental results: Treatment with golden purple rice oil samples at concentrations of 0.0025% and 0.005% inhibited LPS-induced IL-6 secretion in HaCaT cells, with inhibition rates of 23% and 10%, respectively, and both results were statistically significant (P < 0.05).

[0052] Example 2: Tissue morphology detection of samples on a 3D epidermal model Detection method: Hematoxylin-eosin (H&E) staining was used to stain paraffin tissue sections and observe the tissue structure and cell morphology in the skin model. This method can be used to assess the effect of the sample on skin tissue and cells.

[0053] Detection indicators: By comparing the thickness of the epidermis and cell morphology in tissue samples before and after sample addition.

[0054] Group settings The ingredients of Golden Purple Rice Oil are: 33.9% perilla seed oil, 50% rice bran oil, 15% red pine seed oil, 1% patchouli oil, and 0.1% rosemary leaf extract.

[0055] Detection steps Sample application: After culturing the constructed 3D surface skin model for 8 days, a control group and a sample group were set up. The control group was a solvent control group (propylene glycol: ethanol = 3:7). The sample groups were treated with 2% and 5% golden purple rice oil dissolved in a solvent (propylene glycol: ethanol = 3:7) and then applied to the surface of the 3D surface skin model, respectively, once a day for 2 consecutive days, at a dosage of 18 μL / cm³. 2 After each treatment, the 3D epidermal model was placed in an incubator for incubation for a total of 48 hours.

[0056] Tissue processing: Tissues were placed in embedding casks and subjected to gradient dehydration in 4% paraformaldehyde, 70% ethanol-water solution, 80% ethanol-water solution, 90% ethanol-water solution, 95% ethanol-water solution, anhydrous ethanol I, anhydrous ethanol II, methylcyclohexane I, and methylcyclohexane II, respectively, for 1 hour per solution. After dehydration, the tissues were embedded in paraffin, trimmed, and cut into 5 μm thick paraffin sections. The paraffin sections were then dewaxed and rehydrated by immersing them in methylcyclohexane I, methylcyclohexane II, anhydrous ethanol, 95% ethanol-water solution, 80% ethanol-water solution, 70% ethanol-water solution, and distilled water for 10 minutes each.

[0057] Staining: After staining in hematoxylin solution for 8 min, the slides were then differentiated in hydrochloric acid-ethanol solution for 2 s and in ammonia solution for 1 min. Next, they were stained with eosin solution for 1 min. Finally, the slides were dehydrated in 70% ethanol-water solution, 95% ethanol-water solution, anhydrous ethanol, and methylcyclohexane solution, and then mounted with neutral resin mounting medium.

[0058] Data analysis: Tissue morphology was observed and photographed using a microscope, and the thickness of the epidermal layer was measured; Relative epidermal thickness (%) = Image processing was performed using Graphpad Prism graphics software, and the results are expressed as Mean ± SD. A t-test was used to compare the results with the control group. *P < 0.05 indicates a statistically significant difference, **P < 0.01 indicates a statistically significant difference, and ***P < 0.001 indicates an extremely statistically significant difference. Judgment criteria: If the relative epidermal thickness (%) is greater than 100%, the sample at this concentration is judged to have a promoting effect on the integrity of skin tissue structure and morphology.

[0059] Experimental results: Compared with the control group, the epidermal thickness of the 3D skin model increased by 38% and 33% respectively after treatment with 2% and 5% golden purple rice oil samples, and the results were statistically significant (P<0.05), indicating that 2% and 5% golden purple rice oil samples can increase the thickness of the epidermal layer of the 3D skin model.

[0060] Example 3: Detection of barrier-related proteins (FLG, CerS3, DSG1) in a 3D skin model Detection method: Frozen tissue sections were stained with immunofluorescence, and the relative contents of target proteins (FLG, CerS3, DSG1) were determined by semi-quantitative technology. This can be used to determine the effect of the test sample on the expression of target proteins.

[0061] Detection index: The relative average fluorescence intensity (%) of the target protein was calculated by comparing it with the control group to detect the change in the relative average fluorescence intensity of the target protein after treatment with different concentrations of samples.

[0062] Group settings The ingredients of Golden Purple Rice Oil are: 33.9% perilla seed oil, 50% rice bran oil, 15% red pine seed oil, 1% patchouli oil, and 0.1% rosemary leaf extract.

[0063] Detection steps Tissue processing: The tissue was placed in OCT tissue embedding medium and rapidly cooled with liquid nitrogen. The embedded tissue block was then placed in a cryostat and cut into sections with a thickness of 5 μm.

[0064] Staining: Sections were fixed with ice-cold methanol at -20°C, washed three times with DPBS, and incubated with blocking buffer at room temperature for 1 h. After incubation, the corresponding primary antibodies (FLG, CerS3, DSG1) were added, and the sections were incubated overnight at 4°C. The next day, the sections were washed three times with DPBS, and the corresponding secondary antibodies were added. The sections were then incubated at room temperature in the dark for 1.5 h. Finally, the sections were mounted with anti-fluorescence attenuation mounting medium, and photographed under the same exposure conditions using a fluorescence microscope. The relative average fluorescence intensity of the relevant proteins in the skin tissue was analyzed using software.

[0065] Data analysis: The relative average fluorescence intensity (%) was calculated using the following formula. Relative average fluorescence intensity (%) = Image processing was performed using Graphpad Prism graphics software, and the results are expressed as Mean ± SD. A t-test was used to compare the results with the control group. *P < 0.05 indicates a statistically significant difference, **P < 0.01 indicates a statistically significant difference, and ***P < 0.001 indicates an extremely statistically significant difference. Judgment criteria: If the relative average fluorescence intensity (%) is >100%, then the sample at this concentration is judged to be able to increase the relative average fluorescence intensity of the relevant protein.

[0066] Experimental results: After treatment with 2% and 5% golden purple rice oil samples, the relative fluorescence intensity of FLG protein increased by 12% and 16%, respectively, and the results were statistically significant (P < 0.05). Treatment with 2% and 5% golden purple rice oil samples increased the relative fluorescence intensity of DSG1 protein by 16% and 27%, respectively, and the results were statistically significant (P < 0.05).

[0067] in conclusion: Investigate the safe concentration of the sample Cytotoxicity assays showed that the samples at concentrations ranging from 0.00078% to 0.05% exhibited no potential cytotoxicity to HaCaT cells. Based on these results, subsequent experiments in HaCaT cells will be conducted within this concentration range.

[0068] Investigate the effect of the sample on the secretion of related cytokines ELISA results showed that treatment with 0.0025% and 0.005% golden purple rice oil samples could inhibit LPS-induced IL-6 secretion in HaCaT cells, with inhibition rates of 23% and 10%, respectively, and both results were statistically significant (P < 0.05).

[0069] Investigating the effect of samples on the tissue morphology of 3D skin models H&E staining results showed that, compared with the control group, the epidermal thickness of the 3D epidermal model increased by 38% and 33% respectively after treatment with 2% and 5% golden purple rice oil samples, and the results were statistically significant (P<0.05), indicating that 2% and 5% golden purple rice oil samples can increase the thickness of the epidermal layer of the 3D skin model.

[0070] Investigating the effect of samples on the relative fluorescence intensity of barrier-related proteins in a 3D epidermal model Immunofluorescence assay results showed that treatment with 2% and 5% golden purple rice oil samples enhanced the relative fluorescence intensity of filaggrin (FLG), ceramide synthase 3 (CerS3), and desmosome core glycoprotein (DSG1), which play an important role in skin barrier function, and the results were statistically different from those of the control group (P < 0.05).

[0071] In summary, golden purple rice oil can inhibit LPS-induced IL-6 secretion in HaCaT cells, increase the thickness of the epidermis in a 3D skin model, and promote the expression of filaggrin (FLG), ceramide synthase 3 (CerS3), and desmosome core glycoprotein (DSG1), which play important roles in skin barrier function. This indicates that golden purple rice oil can alleviate the inflammatory response of HaCaT cells stimulated by LPS and enhance skin barrier function.

[0072] Example 4: Determination of total unsaturated fatty acids, oleic acid, linoleic acid, and linolenic acid The ingredients of Golden Purple Rice Oil are 33.9% perilla seed oil, 50% rice bran oil, 15% red pine seed oil, 1% patchouli oil, and 0.1% rosemary leaf extract.

[0073] The experimental results are shown in Table 4 below. Table 4 Test Results Example 5: Other formulations of golden purple rice oil Several formulations of golden purple rice oil were prepared, and consumer tests all showed excellent skin adhesion and effects such as repairing the skin barrier, anti-inflammatory and soothing, rapid redness reduction, improved elasticity, anti-wrinkle and firming, and comprehensive nourishment and rejuvenation. Formulas 1, 5, 8, 11, 12, 13, and 16 were added to cosmetic base formulations and consumer tests were conducted, showing the same actual effects. The tested formulations are shown in Tables 5-1, 5-2, 5-3, 5-4, and 5-5 below. Table 5-1 Table 5-2 Table 5-3 Table 5-4 Table 5-5 The weight ratio of ω-9 fatty acids : ω-6 fatty acids : ω-3 fatty acids, calculated as fatty acids, was: Formula 7 is 5:5:1; Formula 12 is 2:2:5; Formula 29 is 2:4:1; Formula 30 is 3:3:2.

[0074] Example 6: Formulation of a personal care composition using golden purple rice oil Formulas 1, 5, 7, 8, 11, 12, 13, 16, 29, and 30 from Example 5 were used as oil mixtures and added to the following personal care composition formulations to prepare personal care compositions.

[0075] Preparation process: 1. Preparation of aqueous phase: Mix deionized water, glycerol and propylene glycol, heat to 70-75℃ and stir to dissolve.

[0076] 2. Oil phase preparation: Heat the above oil mixture and cyclopentamethoxysiloxane to 75°C, and add polysorbate-20 for emulsification.

[0077] 3. Emulsification and mixing: Slowly add the oil phase to the water phase, stir homogenously (2000-3000 rpm) for 5 minutes, and add preservatives and fragrances after cooling to 45℃.

[0078] 4. Post-treatment: Adjust the pH to 5.5-6.0, stir and cool to room temperature before discharging.

[0079] Table 5-7 Example 7: Comparison of Golden Purple Rice Oil with its Components The 5% golden purple rice oil (formula 30) in Examples 2 and 3 was replaced with 5% formula 7, 5% formula 12, or 5% formula 29, and 5% rice bran oil, 5% red pine seed oil, or 5% perilla seed oil in a propylene glycol:ethanol = 3:7 solution, respectively. These solutions were then compared with 0.5% linoleic acid or 0.5% linolenic acid in a propylene glycol:ethanol = 3:7 solution, and their DSG-1 fluorescence intensity values ​​under the experimental conditions of Example 3 were compared.

[0080] Test results are as follows Figure 10 As shown, the golden purple rice oil demonstrates significantly better efficacy in alleviating the inflammatory response of LPS-stimulated HaCaT cells and enhancing skin barrier function compared to other formulations. Furthermore, the formulations in each example also exhibit significantly better efficacy in alleviating the inflammatory response of LPS-stimulated HaCaT cells and enhancing skin barrier function compared to the constituent oils or their corresponding pure fatty acid substances.

[0081] Table 5-8 Relative Average Fluorescence Intensity of DSG-1 The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. An oil and fat composition, characterized in that, It contains monounsaturated fatty acids and polyunsaturated fatty acids, wherein the monounsaturated fatty acids are ω-9 fatty acids; and the polyunsaturated fatty acids are a mixture of ω-6 fatty acids and ω-3 fatty acids.

2. The oil composition according to claim 1, characterized in that, The ω-6 fatty acid is selected from one or more of linoleic acid, γ-linolenic acid, arachidonic acid, and conjugated linoleic acid; the ω-3 fatty acid is selected from one or more of α-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid, and docosahexaenoic acid; and the ω-9 fatty acid is selected from one or more of oleic acid, palmitoleic acid, erucic acid, cod oleic acid, and cis-15-docosahexaenoic acid.

3. The oil composition according to claim 2, characterized in that, The weight ratio of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid is 0.3~4:0.3~4:

1.

4. The oil composition according to claim 3, characterized in that, The weight ratio of the ω-9 fatty acid, ω-6 fatty acid, and ω-3 fatty acid is 1~4:1~4:

1.

5. The oil composition according to claim 4, characterized in that, It contains perilla seed oil, rice bran oil, red pine seed oil, and patchouli oil.

6. The oil composition according to claim 5, characterized in that, The composition also contains rosemary leaf extract.

7. The oil composition according to claim 6, characterized in that, It contains the following components by weight percentage: 10%~50% perilla seed oil, 30%~80% rice bran oil, 2%~30% red pine seed oil, and 0.1%~10% patchouli oil.

8. The oil and fat composition according to claim 7, characterized in that, The composition also contains 0.005% to 1% rosemary leaf extract.

9. The use of the oil composition according to any one of claims 1 to 8 in the preparation of cosmetics, skin care products or pharmaceutical preparations that increase epidermal thickness, reduce wrinkles and firm, and have anti-inflammatory and soothing effects.

10. A personal care composition, characterized in that, The oil and fat composition according to any one of claims 1-8 contains, based on a total amount, 0.01 to 20% by weight.

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