A moisturizing whitening anti-aging cosmetic composition containing a hyaluronic acid derivative and glabridin and application thereof

Abstract

The present application relates to a kind of moisturizing whitening anti-aging cosmetic composition containing hyaluronic acid derivative and glabridin and its application, belong to the field of cosmetics.The cosmetic composition includes glabridin and hyaluronic acid derivative;Wherein, the hyaluronic acid derivative includes hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt and silanized hyaluronic acid or its salt.The present application simultaneously uses hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt and silanized hyaluronic acid or its salt as hyaluronic acid derivative, greatly improve the stability and moisturizing, whitening, anti-aging efficacy of the cosmetic composition provided by the present application, with unexpected synergistic effect.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, specifically to a moisturizing, whitening, and anti-aging cosmetic composition containing hyaluronic acid derivatives and glycyrrhizin, and its application. Background Technology

[0002] Currently, most skin whitening solutions on the market rely on a single active ingredient or a simple combination of ingredients, and their whitening effects and overall performance still have the following shortcomings: 1. Limited synergistic effect due to single active whitening ingredients. Traditional whitening solutions often rely on single active ingredients, such as arbutin, retinoic acid, hydroquinone (HQ), and niacinamide. These ingredients often exhibit insufficient synergistic effects during the whitening process. 2. Limited moisturizing ability affects whitening effects. During the whitening process, the integrity of the skin barrier function has a significant impact on the absorption and effectiveness of whitening ingredients. However, existing whitening solutions often neglect the synergistic effect of moisturizing and whitening; 3. There are concerns about irritation and safety. Whitening ingredients (such as high-concentration retinoic acid and hydroquinone) are highly irritating, and long-term use may lead to skin redness, sensitivity, or even inflammatory reactions. Current solutions lack synergistic ingredients that can effectively reduce irritation and improve safety.

[0003] Hyaluronic acid (HA) is a natural polysaccharide moisturizing factor with wide applications in the cosmetics industry. It can absorb hundreds of times its own weight in water to form a hydrating film that locks in skin moisture and improves dryness. It also maintains skin's moisture balance through deep hydration, increasing skin hydration levels. Furthermore, hyaluronic acid promotes the repair of the skin barrier function, enhances the skin's self-protective ability, and reduces damage from external stimuli. Its soothing effect relieves skin inflammation and irritation, making it especially suitable for sensitive skin. Simultaneously, hyaluronic acid also has multiple benefits, including improving skin tone and promoting collagen synthesis, thus delaying the skin aging process.

[0004] However, traditional hyaluronic acid still has the following limitations in practical applications: its molecular weight is large, its permeability is insufficient, and it is difficult to penetrate deep into the dermis, which limits its deep moisturizing and repairing effects; it is easily degraded under high temperature, acid and alkaline environments or oxidative conditions, and its stability is poor; in addition, its functionality is relatively simple, making it difficult to meet consumers' needs for multiple effects, and its compatibility with other active ingredients is poor, affecting product performance.

[0005] Therefore, there is still an urgent need for a cosmetic composition that has good whitening and moisturizing effects, low irritation, high safety, and good stability. Summary of the Invention

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: In a first aspect, the present invention provides a cosmetic composition.

[0007] A cosmetic composition comprising the following active ingredients: glycyrrhizin and a hyaluronic acid derivative; The hyaluronic acid derivatives include hydrolyzed hyaluronic acid or its salts, acetylated hyaluronic acid or its salts, and silanized hyaluronic acid or its salts. On one hand, the four active ingredients in this invention—glycyrrhizin, hydrolyzed hyaluronic acid or its salts, acetylated hyaluronic acid or its salts, and silanized hyaluronic acid or its salts—work synergistically to achieve unexpected enhanced effects in moisturizing, whitening, and anti-aging. On the other hand, the three components—hydrolyzed hyaluronic acid or its salts, acetylated hyaluronic acid or its salts, and silanized hyaluronic acid or its salts—synergistically improve the stability of glycyrrhizin. For example, when simultaneously containing hydrolyzed hyaluronic acid or its salts, acetylated hyaluronic acid or its salts, and silanized hyaluronic acid or its salts, the content of glycyrrhizin does not change significantly under high temperature and light conditions, and the appearance and properties of the resulting cosmetic composition remain transparent, clear, and odorless, exhibiting unexpected technical effects.

[0008] Hydrolyzed hyaluronic acid (Hyaluronic Acid with Low Molecular Weight): Hydrolyzed hyaluronic acid is produced by enzymatic or chemical hydrolysis, reducing the molecular weight of hyaluronic acid to between 1,000 Da and 50,000 Da, or even lower. This reduced molecular weight allows it to penetrate deeper into the skin, improving its moisturizing effect. It also has stronger skin affinity, enabling rapid absorption and improving the skin barrier function.

[0009] Acetylated hyaluronic acid: Acetylated hyaluronic acid modifies hyaluronic acid with acetyl groups, reducing its molecular polarity and thus minimizing skin irritation. It exhibits excellent stability and durability, providing long-lasting moisturizing and repairing effects on the skin.

[0010] Silicon-Modified Hyaluronic Acid: Silicon-modified hyaluronic acid is produced by chemically modifying the hyaluronic acid molecule to introduce silicon-oxygen bonds, giving it better lipophilicity and spreadability. It has better skin adhesion and film-forming properties, forming a flexible protective film on the skin surface. While locking in moisture, it replenishes silicon in the skin's connective tissue, maintaining the normal morphology of fibroblasts and the regular structure of connective tissue. Silicon-modified hyaluronic acid can promote fibroblast proliferation, promote collagen production, enhance skin elasticity, maintain youthful skin, and resist aging.

[0011] Glabridin, a flavonoid extracted from licorice root (Glycyrrhiza glabra), has garnered significant attention for its remarkable skin-whitening effects. Glabridin effectively reduces melanin production and deposition through multiple mechanisms, including inhibiting tyrosinase activity, blocking melanin transport, scavenging free radicals, and regulating inflammatory responses. It also improves pigmentation caused by oxidative stress and inflammation, thus achieving a significant skin-whitening effect. Furthermore, glabridin enhances skin elasticity, improves fine lines and wrinkles, and delays skin aging through mechanisms such as antioxidant action (scavenging free radicals), promoting collagen synthesis, inhibiting inflammatory responses, repairing UV damage, and delaying cell aging, demonstrating powerful anti-aging activity.

[0012] In some embodiments, the mass ratio of the hydrolyzed hyaluronic acid or its salt to the acetylated hyaluronic acid or its salt is 1:50-50:1. In some embodiments, the mass ratio of the hydrolyzed hyaluronic acid or its salt to the acetylated hyaluronic acid or its salt is 1:50, 1:45, 1:40, 1:35, 1:30, 1:25, 1:20, 1:15, 1:10, 1:5, 1:1, 5:1, 10:1, 15:1, 20:1, 25:1, 30:1, 35:1, 40:1, 45:1, 50:1, or any value within a range of any two of these values.

[0013] In some embodiments, the mass ratio of the hydrolyzed hyaluronic acid or its salt to the silanized hyaluronic acid or its salt is 1:50-50:1. In some embodiments, the mass ratio of the hydrolyzed hyaluronic acid or its salt to the silanized hyaluronic acid or its salt is 1:50, 1:45, 1:40, 1:35, 1:30, 1:25, 1:20, 1:15, 1:10, 1:5, 1:1, 5:1, 10:1, 15:1, 20:1, 25:1, 30:1, 35:1, 40:1, 45:1, 50:1, or any value within a range of any two of these values.

[0014] In some embodiments, the mass ratio of glycyrrhizin to the hyaluronic acid derivative is 1:1 to 1:60. In some preferred embodiments, the mass ratio of glycyrrhizin to the hyaluronic acid derivative is 1:3 to 1:52. In some preferred embodiments, the mass ratio of glycyrrhizin to the hyaluronic acid derivative is 1:3, 1:5, 1:5.05, 1:7.5, 1:10, 1:10.1, 1:10.4, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, 1:52, or any value within the range of any two of these values.

[0015] In some embodiments, the weight-average molecular weight of the hydrolyzed hyaluronic acid or its salt is selected from 1 kDa to 3 kDa. In some embodiments, the weight-average molecular weight of the hydrolyzed hyaluronic acid or its salt is selected from 1 kDa, 1.5 kDa, 2 kDa, 2.5 kDa, 3 kDa, or any value within the range of any two of these values. In some preferred embodiments, the weight-average molecular weight of the hydrolyzed hyaluronic acid or its salt is 2 kDa, and the molecular weight distribution range is 1 kDa-3 kDa. Compared with other weight-average molecular weights of hydrolyzed hyaluronic acid or its salt, the present invention preferably uses a weight-average molecular weight of 1 kDa-3 kDa, and more preferably 2 kDa, which is more beneficial to improving the stability of glycyrrhizin and the moisturizing, whitening, and anti-aging effects of the resulting cosmetic composition.

[0016] In some embodiments, the weight-average molecular weight of the acetylated hyaluronic acid or its salt is selected from 8 kDa to 12 kDa. In some embodiments, the weight-average molecular weight of the acetylated hyaluronic acid or its salt is selected from 8 kDa, 9 kDa, 10 kDa, 11 kDa, 12 kDa, or any value within the range of any two of these values. In some preferred embodiments, the weight-average molecular weight of the acetylated hyaluronic acid or its salt is 10 kDa; the molecular weight distribution range is 8 kDa-12 kDa. Compared to other weight-average molecular weights of acetylated hyaluronic acid or its salt, the present invention preferably uses 8 kDa-12 kDa, and more preferably uses acetylated hyaluronic acid or its salt with a weight-average molecular weight of 10 kDa, which is more beneficial to improving the stability of glycyrrhizin and the moisturizing, whitening, and anti-aging effects of the resulting cosmetic composition.

[0017] In some embodiments, the weight-average molecular weight of the silanized hyaluronic acid or its salt is selected from 1,000,000 Da to 1,500,000 Da. In some embodiments, the weight-average molecular weight of the silanized hyaluronic acid or its salt is selected from 1,000,000 Da, 1,050,000 Da, 1,100,000 Da, 1,150,000 Da, 1,200,000 Da, 1,250,000 Da, 1,300,000 Da, 1,350,000 Da, 1,400,000 Da, 1,450,000 Da, 1,500,000 Da, or any value within any two of these ranges. In some preferred embodiments, the weight-average molecular weight of the silanized hyaluronic acid or its salt is 1,250,000 Da; the molecular weight distribution range is 1,000,000 to 1,500,000 Da. Compared to other weight-average molecular weights of silanized hyaluronic acid or its salt, the present invention preferably uses a weight-average molecular weight of 1,000,000 to 1,500,000 Da, and more preferably a weight-average molecular weight of 1,250,000 Da, which is more conducive to improving the stability of glycyrrhizin and the moisturizing, whitening, and anti-aging effects of the resulting cosmetic composition.

[0018] In some embodiments, the silanized hyaluronic acid or its salt comprises dimethylsilanol hyaluronic acid ester.

[0019] In some embodiments, the salt of the acetylated hyaluronic acid includes sodium acetylated hyaluronic acid.

[0020] In some embodiments, the salt in any of the aforementioned hyaluronic acid derivatives is a cosmetically acceptable salt.

[0021] In some embodiments, the cosmetic composition further includes cosmetically acceptable excipients.

[0022] In some embodiments, the cosmetically acceptable excipients include at least one of solvents and solubilizers.

[0023] In some embodiments, cosmetically acceptable excipients include solvents and solubilizers.

[0024] In some embodiments, cosmetically acceptable excipients include solvents, solubilizers, and other excipients.

[0025] In some embodiments, the other excipients include at least one of the following: emulsifiers, thickeners, stabilizers, humectants, antioxidants, preservatives, skin feel modifiers, pH modifiers, fragrances, pigments, penetration enhancers, and sunscreen excipients.

[0026] In some embodiments, the solubilizer includes at least one of 1,3-butanediol, 1,3-propanediol, and dipropylene glycol.

[0027] In some embodiments, the solvent includes water.

[0028] In some embodiments, the content of glycyrrhizin, calculated based on the total mass of the cosmetic composition, is 0.01 wt% to 0.2 wt%. In some embodiments, the content of glycyrrhizin, calculated based on the total mass of the cosmetic composition, is 0.01 wt%, 0.05 wt%, 0.1 wt%, 0.15 wt%, 0.2 wt%, or any value within the range of any two of these values.

[0029] In some embodiments, the content of the hydrolyzed hyaluronic acid or its salt, calculated by the total mass of the cosmetic composition, is 0.01 wt%-0.5 wt%. In some embodiments, the content of the hydrolyzed hyaluronic acid or its salt, calculated by the total mass of the cosmetic composition, is 0.01 wt%, 0.05 wt%, 0.1 wt%, 0.15 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, or any value within the range of any two of these values. Compared to other contents of hydrolyzed hyaluronic acid or its salt, the use of 0.01 wt%-0.5 wt% of hydrolyzed hyaluronic acid or its salt in this invention is more beneficial in improving the stability of glycyrrhizin and the moisturizing, whitening, and anti-aging effects of the resulting cosmetic composition.

[0030] In some embodiments, the content of acetylated hyaluronic acid or its salt, calculated by the total mass of the cosmetic composition, is 0.01 wt%-0.5 wt%. In some embodiments, the content of acetylated hyaluronic acid or its salt, calculated by the total mass of the cosmetic composition, is 0.01 wt%, 0.05 wt%, 0.1 wt%, 0.15 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, or any value within the range of any two of these values. Compared to other contents of acetylated hyaluronic acid or its salt, the use of 0.01 wt%-0.5 wt% of acetylated hyaluronic acid or its salt in this invention is more beneficial in improving the stability of glycyrrhizin and the moisturizing, whitening, and anti-aging effects of the resulting cosmetic composition.

[0031] In some embodiments, the content of the silanized hyaluronic acid or its salt, calculated by the total mass of the cosmetic composition, is 0.01 wt%-0.5 wt%. In some embodiments, the content of the silanized hyaluronic acid or its salt, calculated by the total mass of the cosmetic composition, is 0.01 wt%, 0.05 wt%, 0.1 wt%, 0.15 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, or any value within the range of any two of these values. Compared to other contents of the silanized hyaluronic acid or its salt, the use of 0.01 wt%-0.5 wt% of the silanized hyaluronic acid or its salt in this invention is more conducive to improving the stability of glycyrrhizin and the moisturizing, whitening, and anti-aging effects of the resulting cosmetic composition.

[0032] In some embodiments, the content of the solubilizer is 2wt%-20wt% based on the total mass of the cosmetic composition. In some embodiments, the content of the solubilizer is 2wt%, 3wt%, 4wt%, 5wt%, 6wt%, 7wt%, 8wt%, 9wt%, 10wt%, 15wt%, 20wt%, or any value within the range of any two of these values, based on the total mass of the cosmetic composition.

[0033] In some embodiments, the solvent content is 78 wt%-98 wt% based on the total mass of the cosmetic composition. In some embodiments, the solvent content is 78.3 wt%-97.96 wt% based on the total mass of the cosmetic composition. In some embodiments, the solvent content is 78 wt%, 78.3 wt%, 80 wt%, 85 wt%, 90 wt%, 90.79 wt%, 90.89 wt%, 91 wt%, 91.43 wt%, 91.47 wt%, 91.5 wt%, 92 wt%, 93 wt%, 94 wt%, 95 wt%, 97.96 wt%, 98 wt%, or any value within a range of any two of these values, based on the total mass of the cosmetic composition.

[0034] In some embodiments, the cosmetic composition includes a solubilizer and a solvent, the solubilizer comprising 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of glycyrrhizin is 0.01wt%-0.2wt%, the content of hydrolyzed hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of acetylated hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of silanized hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of the solubilizer is 2wt%-20wt%, and the content of the solvent is 78wt%-98wt%.

[0035] In some embodiments, the cosmetic composition includes a solubilizer and a solvent, the solubilizer comprising 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of glycyrrhizin is 0.01wt%-0.2wt%, the content of hydrolyzed hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of acetylated hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of silanized hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of the solubilizer is 2wt%-20wt%, and the content of the solvent is 78.3wt%-97.96wt%.

[0036] In some embodiments, the cosmetic composition includes a solubilizer and a solvent, the solubilizer including 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.01 wt%, the content of the acetylated hyaluronic acid or its salt is 0.01 wt%, the content of the silanized hyaluronic acid or its salt is 0.01 wt%, the content of glycyrrhizin is 0.01 wt%, the content of the solubilizer is 2 wt%, and the balance is solvent.

[0037] In some embodiments, the cosmetic composition includes a solubilizer and a solvent, the solubilizer including 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.01 wt%, the content of the acetylated hyaluronic acid or its salt is 0.5 wt%, the content of the silanized hyaluronic acid or its salt is 0.01 wt%, the content of glycyrrhizin is 0.01 wt%, the content of the solubilizer is 8 wt%, and the balance is solvent.

[0038] In some embodiments, the cosmetic composition includes a solubilizer and a solvent, the solubilizer including 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.01 wt%, the content of the acetylated hyaluronic acid or its salt is 0.01 wt%, the content of the silanized hyaluronic acid or its salt is 0.5 wt%, the content of glycyrrhizin is 0.05 wt%, the content of the solubilizer is 8 wt%, and the balance is solvent.

[0039] In some embodiments, the cosmetic composition includes a solubilizer and a solvent, the solubilizer including 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.5 wt%, the content of the acetylated hyaluronic acid or its salt is 0.5 wt%, the content of the silanized hyaluronic acid or its salt is 0.01 wt%, the content of glycyrrhizin is 0.1 wt%, the content of the solubilizer is 8 wt%, and the balance is solvent.

[0040] In some embodiments, the cosmetic composition includes a solubilizer and a solvent, the solubilizer including 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.5 wt%, the content of the acetylated hyaluronic acid or its salt is 0.01 wt%, the content of the silanized hyaluronic acid or its salt is 0.5 wt%, the content of glycyrrhizin is 0.2 wt%, the content of the solubilizer is 8 wt%, and the balance is solvent.

[0041] In some embodiments, the cosmetic composition includes a solubilizer and a solvent, the solubilizer including 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.5 wt%, the content of the acetylated hyaluronic acid or its salt is 0.5 wt%, the content of the silanized hyaluronic acid or its salt is 0.5 wt%, the content of glycyrrhizin is 0.2 wt%, the content of the solubilizer is 20 wt%, and the balance is solvent.

[0042] In some embodiments, in any of the aforementioned cosmetic compositions, the hyaluronic acid derivative is hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid.

[0043] Secondly, the present invention provides a method for preparing the aforementioned cosmetic composition.

[0044] A method for preparing the cosmetic composition according to the first aspect, characterized by comprising the following steps: (1) Dissolve licorice root extract in a solubilizer to obtain solution A; (2) Mix solution A with hyaluronic acid derivative and solvent, stir until completely dissolved, filter, and obtain the cosmetic composition; or mix solution A with hyaluronic acid derivative, solvent and other excipients, stir until completely dissolved, filter, and obtain the cosmetic composition.

[0045] In some embodiments, step (1) includes: mixing glycyrrhizin with a solubilizer, heating to 50°C-70°C, and stirring to dissolve. In some embodiments, step (1) includes: mixing glycyrrhizin with a solubilizer, heating to 50°C, 55°C, 60°C, 65°C, or 70°C, and stirring to dissolve.

[0046] Thirdly, the present invention provides an application of the aforementioned cosmetic composition or the cosmetic composition prepared by the aforementioned preparation method.

[0047] The use of a cosmetic composition as described in the first aspect or a cosmetic composition prepared by the preparation method described in the second aspect in the preparation of cosmetics.

[0048] In some embodiments, the cosmetic is used for moisturizing, repairing, whitening, and / or anti-aging.

[0049] In some embodiments, the dosage form of the cosmetic is a serum, tincture, lotion, gel, emulsion, oil, cream, or mask.

[0050] Fourthly, the present invention provides a cosmetic product.

[0051] A cosmetic product comprising the cosmetic composition described in the first aspect or the cosmetic composition prepared by the preparation method described in the second aspect.

[0052] In some embodiments, the cosmetic is used for moisturizing, repairing, whitening, and / or anti-aging.

[0053] In some embodiments, the dosage form of the cosmetic is a serum, tincture, lotion, gel, emulsion, oil, cream, or mask.

[0054] Beneficial effects Compared with the prior art, at least one of the following beneficial effects is present in a certain embodiment of the present invention: (1) Multifunctionality of synergistic effect This invention overcomes the limitations of traditional hyaluronic acid in terms of permeability, stability, and functionality through the scientific combination of various hyaluronic acid derivatives. Hydrolyzed hyaluronic acid or its salts, acetylated hyaluronic acid or its salts, and silanized hyaluronic acid or its salts provide multi-layered moisturizing, repairing, and barrier-strengthening effects tailored to different skin types. Simultaneously, the introduction of glycyrrhizin significantly enhances whitening and antioxidant properties. The synergistic effect of these multiple ingredients not only achieves a "1+1+1+1>4" synergistic effect but also significantly improves the stability and compatibility of glycyrrhizin, resulting in better compatibility and synergy in the formulation, thereby comprehensively improving the overall performance and stability of the product.

[0055] On the one hand, the four active ingredients in this invention—glycyrrhizin, hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt, and silanized hyaluronic acid or its salt—work synergistically to achieve unexpected technical effects in moisturizing, whitening, and anti-aging. On the other hand, the three components—hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt, and silanized hyaluronic acid or its salt—synergistically improve the stability of glycyrrhizin. For example, when containing hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt, and silanized hyaluronic acid or its salt, the content of glycyrrhizin does not change significantly under high temperature and light conditions, and the appearance and properties of the resulting cosmetic composition remain transparent, clear, and odorless, exhibiting unexpected technical effects.

[0056] (2) Significantly improved efficacy Moisturizing effect: By constructing a multi-layered moisturizing network, it significantly increases the skin's moisture content, meeting the moisturizing needs of different skin types.

[0057] Whitening effect: Inhibits melanin production, improves uneven skin tone, and presents even and radiant skin; Anti-aging effect: Promotes collagen production, reduces fine lines and wrinkles, and slows down the skin aging process.

[0058] (3) Improved component stability and permeability This invention significantly improves the stability and permeability of each component in the formulation by introducing silanized hyaluronic acid or its salt, enhancing the overall performance and durability of the formulation, and enabling the product to maintain a highly efficient and stable effect during long-term use.

[0059] (4) Wide applicability The formulation design of this invention has good applicability and is suitable for various types of cosmetics (such as serums, creams, masks, etc.), which can meet different skin types and skin care needs, showing great market application potential.

[0060] In summary, this invention, through scientific formulation and functional complementarity, not only overcomes the shortcomings of single ingredients or simple combinations in existing technologies, but also significantly improves the overall efficacy and stability of the product, providing an innovative technical solution for developing efficient and multifunctional skincare products.

[0061] Terminology Explanation In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0062] The terms “room temperature” or “normal temperature” refer to ambient temperature, which is approximately 10°C to approximately 35°C, approximately 10°C to approximately 30°C, or approximately 20°C to 30°C, or approximately 25°C.

[0063] The term "wt%" indicates a percentage by mass.

[0064] The term "rpm" stands for the unit of rotational speed, "revolutions per minute".

[0065] The term "v / v" indicates a volume ratio.

[0066] The term "Mw" refers to the weight-average molecular weight.

[0067] The term "uniform molecular weight protein" refers to a protein with a uniform molecular weight, whose molecular structure, amino acid sequence and modification are highly consistent, and therefore appear as a single molecular weight band or peak in analytical methods such as electrophoresis and mass spectrometry.

[0068] The term "single molecular weight" refers to the molecular mass value of a protein molecule that is homogeneous in terms of physicochemical properties. That is, the mass of each molecule in the protein group is completely consistent or the difference is very small (usually within the accuracy of mass spectrometry detection, such as ±0.1%~1%).

[0069] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0070] In the following content, all figures disclosed herein, whether or not they use the words "approximately" or "about," are approximate values. The value of each figure may vary by 1%, 2%, 5%, 7%, 8%, 10%, 15%, or 20%. Whenever a figure with a value of N is disclosed, any figure with a value of N+ / -1%, N+ / -2%, N+ / -3%, N+ / -5%, N+ / -7%, N+ / -8%, N+ / -10%, N+ / -15%, or N+ / -20% will be explicitly disclosed, where "+ / -" indicates addition or subtraction. Detailed Implementation

[0071] To enable those skilled in the art to better understand the technical solutions of the present invention, some non-limiting embodiments are further disclosed below to provide a more detailed description of the present invention.

[0072] All reagents used in this invention can be purchased from the market or prepared by conventional methods in the prior art, or can be prepared by the methods described in this invention.

[0073] I. Sources of some reagents used in the embodiments and comparative examples of this invention Unless otherwise stated, some of the reagents used in the embodiments and comparative examples of this invention are from the following sources: Hydrolyzed hyaluronic acid (Mw 2kDa): Trade name Hybloom™ hydrolyzed hyaluronic acid (HA-TLM 3-5), manufactured by Bloomage Biotechnology Co., Ltd., with a molecular weight distribution of 1k-3kDa.

[0074] Acetylated sodium hyaluronate (Mw 10kDa): Acetylated sodium hyaluronate, trade name PRIMALHYALULTRAFILLER, is manufactured by Givaudan Fragrance & Flavor (Shanghai) Co., Ltd., with a molecular weight distribution of 8k-12kDa.

[0075] Silanized hyaluronic acid (Mw 1.25 million Da): Dimethylsilanol hyaluronic acid ester, manufactured by Shandong Focus Freda Biotechnology Co., Ltd., with a molecular weight ranging from 1 million to 1.5 million Da.

[0076] II. Method for Determination of Glycyrrhizin Content The test method for glycyrrhizin content refers to Q / FZR 0101-2023 "Glycyrrhizin as a Raw Material for Cosmetics", and the specific chromatographic conditions are as follows: a) Column: Z0RBAX StableBond C18, 4.6x250mm, 5um; b) Flow rate: 1.0 mL / min; c) Mobile phase: acetonitrile:water = 80:20, filtered using a 0.22 pm membrane filter; d) Injection volume: 10 μL; e) Column temperature: 25℃; f) Wavelength: 280nm.

[0077] Examples 1-4: Preparation of cosmetic compositions Formula: See Table 1.

[0078] Table 1: Formulation of cosmetic compositions

[0079] Preparation method: (1) Mix the solubilizer 1,3-butanediol and glycyrrhizin and heat to 60°C, stirring until completely dissolved to obtain solution A; (2) Mix solution A with hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and water, stir until completely dissolved, filter, and obtain the composition.

[0080] Comparative Examples 1-3: Investigation of single hyaluronic acid derivatives (total content of hyaluronic acid derivatives remains unchanged) Comparative Example 1: The only difference from Example 1 is that acetylated sodium hyaluronate (Mw 10kDa) and silanized hyaluronic acid (Mw 1.25 million Da) are replaced with hydrolyzed hyaluronic acid (Mw 1kDa), and the other conditions are the same as in Example 1.

[0081] Comparative Example 2: The only difference from Example 1 is that hydrolyzed hyaluronic acid (Mw 1kDa) and silanized hyaluronic acid (Mw 1.25 million Da) are replaced with acetylated sodium hyaluronate (Mw 10kDa), and the other conditions are the same as in Example 1.

[0082] Comparative Example 3: The only difference from Example 1 is that hydrolyzed hyaluronic acid (Mw 1kDa) and acetylated sodium hyaluronate (Mw 10kDa) are replaced with silanized hyaluronic acid (Mw 1.25 million Da), and the other conditions are the same as in Example 1.

[0083] Comparative Examples 4-6: Investigation of Two Hyaluronic Acid Derivatives Comparative Example 4: The difference from Example 1 is that hydrolyzed hyaluronic acid (Mw 1kDa) was replaced with acetylated sodium hyaluronate (Mw 10kDa, manufacturer: Givaudan Fragrance & Flavor (Shanghai) Co., Ltd.), and the other conditions are the same as in Example 1.

[0084] Comparative Example 5: The difference from Example 1 is that acetylated sodium hyaluronate (Mw 10kDa) was replaced with silanized hyaluronic acid (Mw 1.25 million Da, manufacturer: Shandong Focus Freda Biotechnology Co., Ltd.), and the other conditions are the same as in Example 1.

[0085] Comparative Example 6: The difference from Example 1 is that silanized hyaluronic acid (Mw 1.25 million Da) was replaced with hydrolyzed hyaluronic acid (Mw 1 kDa, manufacturer: Bloomage Biotechnology Co., Ltd.), and the other conditions are the same as in Example 1.

[0086] Comparative Examples 7-9: Investigation of Hyaluronic Acid Derivative Types Comparative Example 7: The difference from Example 1 is that hydrolyzed hyaluronic acid (Mw 1kDa) was replaced with hyaluronic acid (Mw 50kDa, manufacturer: Bloomage Biotechnology Co., Ltd.), and the other conditions are the same as in Example 1.

[0087] Comparative Example 8: The difference from Example 1 is that acetylated sodium hyaluronate (Mw 10kDa) was replaced with hyaluronic acid (Mw 50kDa, manufacturer: Bloomage Biotechnology Co., Ltd.), and the other conditions are the same as in Example 1.

[0088] Comparative Example 9: The difference from Example 1 is that silanized hyaluronic acid (Mw 1.25 million Da) was replaced with hyaluronic acid (Mw 50 kDa, manufacturer: Bloomage Biotechnology Co., Ltd.), and the other conditions are the same as in Example 1.

[0089] Comparative Examples 10-11: Molecular Weight Investigation of Hydrolyzed Hyaluronic Acid Comparative Example 10: The difference from Example 1 is that hydrolyzed hyaluronic acid was replaced with hydrolyzed hyaluronic acid with a weight-average molecular weight of <1kDa (manufacturer: Bloomage Biotechnology Co., Ltd.), and the other conditions were the same as in Example 1.

[0090] Comparative Example 11: The difference from Example 1 is that hydrolyzed hyaluronic acid was replaced with hydrolyzed hyaluronic acid with a weight-average molecular weight of 20 kDa (manufacturer: Bloomage Biotechnology Co., Ltd.), and the other conditions were the same as in Example 1.

[0091] Comparative Examples 12-13: Molecular Weight Investigation of Acetylated Sodium Hyaluronate Comparative Example 12: The difference from Example 1 is that acetylated sodium hyaluronate was replaced with acetylated sodium hyaluronate with a weight-average molecular weight of 5 kDa (manufacturer: Bloomage Biotechnology Co., Ltd.), and the other conditions were the same as in Example 1.

[0092] Comparative Example 13: The difference from Example 1 is that acetylated hyaluronic acid was replaced with sodium acetylated hyaluronic acid with a weight-average molecular weight of 20 kDa (manufacturer: Bloomage Biotech Co., Ltd.), and the other conditions were the same as in Example 1.

[0093] Comparative Examples 14-15: Molecular Weight Investigation of Silanized Hyaluronic Acid Comparative Example 14: The difference from Example 1 is that the silanized hyaluronic acid was replaced with silanized hyaluronic acid with a weight average molecular weight of 500,000 Da (manufacturer: Shandong Focus Freda Biotechnology Co., Ltd.), and the other conditions were the same as in Example 1.

[0094] Comparative Example 15: The difference from Example 1 is that the silanized hyaluronic acid was replaced with silanized hyaluronic acid with a weight average molecular weight of 2 million Da (manufacturer: Shandong Focus Freda Biotechnology Co., Ltd.), and the other conditions were the same as in Example 1.

[0095] Comparative Examples 16-17: Investigation of the Content of Hydrolyzed Hyaluronic Acid Comparative Example 16: The difference from Example 1 is that the content of hydrolyzed hyaluronic acid was modified to 0.005 wt%, and the water content was adjusted to make the total content of each component 100 wt%. The other conditions are the same as in Example 1.

[0096] Comparative Example 17: The difference from Example 1 is that the content of hydrolyzed hyaluronic acid was modified to 1 wt%, and the water content was adjusted to make the total content of each component 100 wt%. The other conditions are the same as in Example 1.

[0097] Comparative Examples 18-19: Investigation of the Content of Acetylated Sodium Hyaluronate Comparative Example 18: The difference from Example 1 is that the content of acetylated sodium hyaluronate was modified to 0.005 wt%, and the water content was adjusted to make the total content of all components 100 wt%. The other conditions are the same as in Example 1.

[0098] Comparative Example 19: The difference from Example 1 is that the content of acetylated sodium hyaluronate was modified to 1 wt%, and the water content was adjusted to make the total content of all components 100 wt%. The other conditions are the same as in Example 1.

[0099] Comparative Examples 20-21: Investigation of Silanized Hyaluronic Acid Content Comparative Example 20: The difference from Example 1 is that the content of silanized hyaluronic acid was modified to 0.005 wt%, and the water content was adjusted to make the total content of all components 100 wt%. The other conditions are the same as in Example 1.

[0100] Comparative Example 21: The difference from Example 1 is that the content of silanized hyaluronic acid was modified to 1 wt%, and the water content was adjusted to make the total content of all components 100 wt%. The other conditions are the same as in Example 1.

[0101] Comparative Example 22: Without hyaluronic acid derivatives Comparative Example 22: The only difference from Example 1 is that it does not contain hydrolyzed hyaluronic acid (Mw 1kDa), acetylated sodium hyaluronate (Mw 10kDa), or silanized hyaluronic acid (Mw 1.25 million Da), and the water content is adjusted to make the total content of each component 100wt%. All other conditions are the same as in Example 1.

[0102] Comparative Examples 23-25: Single hyaluronic acid derivatives (the content of the single hyaluronic acid derivatives to be investigated remains unchanged). Comparative Example 23: The only difference from Example 1 is that acetylated sodium hyaluronate (Mw 10kDa) and silanized hyaluronic acid (Mw 1.25 million Da) are replaced with water, and the other conditions are the same as in Example 1.

[0103] Comparative Example 24: The only difference from Example 1 is that hydrolyzed hyaluronic acid (Mw 1kDa) and silanized hyaluronic acid (Mw 1.25 million Da) are replaced with water, and the other conditions are the same as in Example 1.

[0104] Comparative Example 25: The only difference from Example 1 is that hydrolyzed hyaluronic acid (Mw 1kDa) and acetylated sodium hyaluronate (Mw 10kDa) are replaced with water, and the other conditions are the same as in Example 1.

[0105] Experimental Example 1: Stability Study The stability of the cosmetic compositions obtained in the above examples and comparative examples was investigated as follows.

[0106] Stability testing was conducted in accordance with T / GDCQMA 002-2023 "Standard for Stability Testing of Cosmetics".

[0107] High-temperature stability test: The compositions obtained in the above examples and comparative examples were placed in sealed containers and subjected to high-temperature conditions (50℃±2℃) for 30 days for stability test. The appearance, odor, glycyrrhizin content, etc. of each example or comparative example after 0 days and 30 days under high-temperature conditions (50℃±2℃) were detected. The results are shown in Table 2 and Table 3.

[0108] Strong light irradiation test: The compositions obtained in the above examples and comparative examples were placed in transparent containers and placed in a light box with an illuminance of 4500 lx ± 500 lx for 30 days. The appearance, odor, glycyrrhizin content, etc. were tested and compared with the results after 0 days. The results are shown in Tables 2 and 3.

[0109] Table 2: Results of the examination on appearance and odor stability

[0110] Table 3: Results of stability test of glycyrrhizin content

[0111] Note: Glycyrrhizin retention rate = (Glycyrrhizin content after 30 days under corresponding conditions ÷ Glycyrrhizin content on day 0) × 100%.

[0112] in conclusion: (1) Experiments show that the examples remained transparent and clear with a glycyrrhizin retention rate >96%, while the comparative examples generally showed discoloration and degradation. This indicates that the combination of triple hyaluronic acid derivatives and glycyrrhizin can enhance the stability of the formulation and reduce the degradation of glycyrrhizin. The stabilization mechanism lies in the scavenging of free radicals by the reducing end of hydrolyzed hyaluronic acid, the chelation of metal ions by acetylated sodium hyaluronate, and the formation of a hydrophobic film by silanized hyaluronic acid to reduce the oxygen diffusion coefficient. The triple protection reduces the degradation rate constant of glycyrrhizin, breaking through the bottleneck of photosensitive ingredient application.

[0113] (2) The results show that, compared with those containing no hydrolyzed hyaluronic acid, acetylated sodium hyaluronate and silanized hyaluronic acid, or containing only one or two of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate and silanized hyaluronic acid, the present invention uses hydrolyzed hyaluronic acid, acetylated sodium hyaluronate and silanized hyaluronic acid at the same time, which is more conducive to improving the stability of glycyrrhizin. The hydrolyzed hyaluronic acid, acetylated sodium hyaluronate and silanized hyaluronic acid synergistically improve the stability of glycyrrhizin, which has unexpected technical effects.

[0114] (3) The results show that, compared with replacing any one of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, or silanized hyaluronic acid with hyaluronic acid, the present invention uses hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid simultaneously, which is more conducive to improving the stability of glycyrrhizin. The hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid synergistically improve the stability of glycyrrhizin, which has unexpected technical effects.

[0115] (4) The results show that, compared with other weight-average molecular weights of hydrolyzed hyaluronic acid, the hydrolyzed hyaluronic acid with a weight-average molecular weight (selected from 1kDa-3kDa; preferably 2kDa) provided by the present invention is more conducive to improving the stability of glycyrrhizin. It can synergistically improve the stability of glycyrrhizin with acetylated sodium hyaluronate (selected from 8kDa-12kDa; preferably 10kDa) and silanized hyaluronic acid (selected from 1 million Da-1.5 million Da; preferably 1.25 million Da), and has unexpected technical effects.

[0116] (5) The results show that, compared with other weight-average molecular weights of acetylated sodium hyaluronate, the weight-average molecular weight of acetylated sodium hyaluronate (selected from 8kDa-12kDa; preferably 10kDa) provided by the present invention is more conducive to improving the stability of glycyrrhizin. It can synergistically improve the stability of glycyrrhizin with hydrolyzed hyaluronic acid (selected from 1kDa-3kDa; preferably 2kDa) and silanized hyaluronic acid (selected from 1 million Da-1.5 million Da, preferably 1.25 million Da), which has unexpected technical effects.

[0117] (6) The results show that, compared with other weight-average molecular weights of silanized hyaluronic acid, the weight-average molecular weight of silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da to 1.5 million Da, preferably 1.25 million Da) provided by the present invention is more conducive to improving the stability of glycyrrhizin. It can synergistically improve the stability of glycyrrhizin with hydrolyzed hyaluronic acid (weight-average molecular weight selected from 1 kDa to 3 kDa; preferably 2 kDa) and acetylated sodium hyaluronate (weight-average molecular weight selected from 8 kDa to 12 kDa; preferably 10 kDa), which has unexpected technical effects.

[0118] (7) The results show that, compared with other contents of any of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid, the present invention uses 0.01wt%-0.5wt% of hydrolyzed hyaluronic acid, 0.01wt%-0.5wt% of acetylated sodium hyaluronate, and 0.01wt%-0.5wt% of silanized hyaluronic acid, which is more conducive to improving the stability of glycyrrhizin and has unexpected technical effects.

[0119] Experimental Example 2: Investigation of Moisturizing Efficacy Using the cosmetic compositions obtained in the above examples or comparative examples as test samples, the following procedures were followed for testing.

[0120] Test Method: A Corneometer® CM825 skin moisture meter was used to test the inner forearm of 160 subjects (5 subjects per group) with dry skin. During the experiment, after cleansing and balancing their skin condition, subjects applied the test sample (2 mg / cm²) to the inner forearm. 2 The changes in stratum corneum moisture content were measured at 1 hour, 4 hours, 8 hours and 24 hours after application, and the results are shown in Table 4.

[0121] Measurement indicators: Record the moisture content value for each test and calculate the average rate of change of moisture content at each time point.

[0122] Judgment criteria: The moisturizing effect is evaluated by measuring the stratum corneum moisture content at different time points after application of the test product. The greater the increase in moisture content and the longer the duration of the increase, the more significant the moisturizing effect of the test product.

[0123] Table 4: Results of Moisture Content Changes

[0124] Note: The results in Table 4 are percentage values ​​of moisture content at different time points compared to the moisture content before use, i.e.: (Moisture content at the corresponding time point ÷ Moisture content before use) ☓100% in conclusion: (1) The results showed that the 24-hour water content of each embodiment increased by more than 30.4%, which was 3.1 times higher than the best single component (9.8%) (p<0.01). Its four-dimensional synergistic mechanism includes hydrolyzed hyaluronic acid to activate the expression of AQP3 aquaporin in the granular layer, acetylated sodium hyaluronate to increase the water holding capacity of the hydration membrane through electrostatic anchoring, silanized hyaluronic acid to form a siloxane hydrophobic network to reduce transdermal water loss, and glycyrrhizin to upregulate filaggrin to promote the generation of natural moisturizing factors. The four components achieve long-lasting moisturizing through a chain reaction of "penetration-anchoring-locking-synthesis".

[0125] (2) The results show that, compared with products that do not contain hydrolyzed hyaluronic acid, acetylated sodium hyaluronate and silanized hyaluronic acid, or products that only contain one or two of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin, the present invention uses hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin simultaneously, which is more conducive to improving the moisturizing effect of the obtained cosmetic composition. Hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin synergistically improve the moisturizing effect of the obtained cosmetic composition, which has unexpected technical effects.

[0126] (3) The results show that, compared with replacing any one of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, or silanized hyaluronic acid with hyaluronic acid, the hyaluronic acid derivative of the present invention, which simultaneously uses hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid, is more conducive to improving the moisturizing effect of the obtained cosmetic composition. Hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid, and glycyrrhizin synergistically improve the moisturizing effect of the obtained cosmetic composition, which has unexpected technical effects.

[0127] (4) The results show that, compared with other weight-average molecular weights of hydrolyzed hyaluronic acid, the hydrolyzed hyaluronic acid with a weight-average molecular weight (selected from 1kDa-3kDa; preferably 2kDa) provided by the present invention is more conducive to improving the moisturizing effect of glycyrrhizin. It can synergistically improve the moisturizing effect of the obtained cosmetic composition with acetylated sodium hyaluronate (weight-average molecular weight selected from 8kDa-12kDa; preferably 10kDa), silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da-1.5 million Da, preferably 1.25 million Da) and glycyrrhizin, and has unexpected technical effects.

[0128] (5) The results show that, compared with other weight-average molecular weights of acetylated sodium hyaluronate, the weight-average molecular weight of acetylated sodium hyaluronate (selected from 8kDa-12kDa; preferably 10kDa) provided by the present invention is more conducive to improving the moisturizing effect of glycyrrhizin. It can synergistically improve the moisturizing effect of the obtained cosmetic composition with hydrolyzed hyaluronic acid (weight-average molecular weight selected from 1kDa-3kDa; preferably 2kDa), silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da-1.5 million Da, preferably 1.25 million Da) and glycyrrhizin, and has unexpected technical effects.

[0129] (6) The results show that, compared with other weight-average molecular weights of silanized hyaluronic acid, the weight-average molecular weight of silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da to 1.5 million Da, preferably 1.25 million Da) provided by the present invention is more conducive to improving the moisturizing effect of glycyrrhizin. It can synergistically improve the moisturizing effect of the obtained cosmetic composition with hydrolyzed hyaluronic acid (weight-average molecular weight selected from 1 kDa to 3 kDa; preferably 2 kDa), acetylated sodium hyaluronate (weight-average molecular weight selected from 8 kDa to 12 kDa; preferably 10 kDa) and glycyrrhizin, and has unexpected technical effects.

[0130] (7) The results show that, compared with other contents of any one of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid, the present invention uses 0.01wt%-0.5wt% of hydrolyzed hyaluronic acid, 0.01wt%-0.5wt% of acetylated sodium hyaluronate, and 0.01wt%-0.5wt% of silanized hyaluronic acid, which is more conducive to improving the moisturizing effect of the obtained cosmetic composition and has unexpected technical effects.

[0131] Experiment 3: Whitening Efficacy Examination Using the cosmetic compositions obtained in the above examples or comparative examples as test samples, the following procedures were followed for testing.

[0132] Test method: Tyrosinase inhibition rate test: The principle is based on the key role of tyrosinase in melanin production. Tyrosinase catalyzes the conversion of tyrosine to dopa, which in turn generates melanin. By inhibiting tyrosinase activity, melanin production can be reduced, thus achieving a whitening effect. Reference standard T / GDCA 006-2021 "Test Method for Inhibition of Tyrosinase Activity by Cosmetic Raw Materials". The positive control was 2% kojic acid, and the detection concentration of the test sample was 0.1 wt%. The results are shown in Table 5.

[0133] Melanin inhibition rate test and melanin synthesis-related protein expression (MITF mRNA) downregulation test: B16 mouse melanoma cells are a commonly used cell model for studying melanin production. This study measured changes in melanin synthesis after the whitening ingredients were applied to melanocytes, and used Western blotting to detect MITF mRNA expression levels to evaluate the inhibitory effect on melanin production. Reference standard: TSHRH 027-2019. In vitro test of B16 cell melanin synthesis inhibition experiment to determine the whitening efficacy of the composition. The positive control was 2% kojic acid, and the detection concentration of the test sample was 0.1 wt%. Results are shown in Table 5.

[0134] Criteria for determining skin whitening efficacy: Based on the above experimental results, the skin whitening efficacy of the test product was comprehensively evaluated from three dimensions: inhibition of tyrosinase activity, melanin inhibition rate, and fold reduction in melanin synthesis-related protein expression (MITF mRNA). Tyrosinase activity inhibition rate: This reflects the ability of the test substance to inhibit the key enzyme in melanin synthesis. The higher the inhibition rate, the more significant the whitening effect.

[0135] Melanin inhibition rate: This reflects the inhibitory effect of the test substance on melanin production. The higher the inhibition rate, the more significant the whitening effect.

[0136] MITF mRNA downregulation fold: This reflects the inhibitory effect of the test substance on melanin synthesis-related proteins. The higher the downregulation fold, the more significant the whitening effect.

[0137] Table 5: Results of Whitening Efficacy Evaluation

[0138] in conclusion: (1) The melanin reduction rate of each embodiment reached more than 38.2%, which is more than 1.8 times that of a single component, and the MITF was reduced by about 2.2 times (reaching 80% effect of 2% kojic acid). The synergistic effect is due to the fact that hydrolyzed hyaluronic acid promotes the transdermal delivery of glycyrrhizin, acetylated sodium hyaluronate inhibits p38 MAPK phosphorylation and blocks inflammatory pigmentation, silanized hyaluronic acid prolongs the epidermal retention time of glycyrrhizin, and glycyrrhizin inhibits tyrosinase activity and regulates MITF transcription at two targets, thus achieving full-pathway whitening from transdermal delivery to signal blocking.

[0139] (2) The results show that, compared with products that do not contain hydrolyzed hyaluronic acid, acetylated sodium hyaluronate and silanized hyaluronic acid, or products that only contain one or two of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin, the present invention uses hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin simultaneously, which is more conducive to improving the whitening effect of the obtained cosmetic composition. Hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin synergistically improve the whitening effect of the obtained cosmetic composition, which has unexpected technical effects.

[0140] (3) The results show that, compared with replacing any one of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, or silanized hyaluronic acid with hyaluronic acid, the hyaluronic acid derivative of the present invention, which simultaneously uses hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid, is more conducive to improving the whitening effect of the obtained cosmetic composition. The hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid, and glycyrrhizin synergistically improve the whitening effect of the obtained cosmetic composition, which has unexpected technical effects.

[0141] (4) The results show that, compared with other weight-average molecular weights of hydrolyzed hyaluronic acid, the hydrolyzed hyaluronic acid with a weight-average molecular weight (selected from 1kDa-3kDa; preferably 2kDa) provided by the present invention is more conducive to improving the whitening effect of glycyrrhizin. It can synergistically improve the whitening effect of the obtained cosmetic composition with acetylated sodium hyaluronate (weight-average molecular weight selected from 8kDa-12kDa; preferably 10kDa), silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da-1.5 million Da, preferably 1.25 million Da) and glycyrrhizin, and has unexpected technical effects.

[0142] (5) The results show that, compared with other weight-average molecular weights of acetylated sodium hyaluronate, the weight-average molecular weight of acetylated sodium hyaluronate (selected from 8kDa-12kDa; preferably 10kDa) provided by the present invention is more conducive to improving the whitening effect of glycyrrhizin. It can synergistically improve the whitening effect of the obtained cosmetic composition with hydrolyzed hyaluronic acid (weight-average molecular weight selected from 1kDa-3kDa; preferably 2kDa), silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da-1.5 million Da, preferably 1.25 million Da) and glycyrrhizin, and has unexpected technical effects.

[0143] (6) The results show that, compared with other weight-average molecular weights of silanized hyaluronic acid, the weight-average molecular weight of silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da to 1.5 million Da, preferably 1.25 million Da) provided by the present invention is more conducive to improving the whitening effect of glycyrrhizin. It can synergistically improve the whitening effect of the obtained cosmetic composition with hydrolyzed hyaluronic acid (weight-average molecular weight selected from 1 kDa to 3 kDa; preferably 2 kDa), acetylated sodium hyaluronate (weight-average molecular weight selected from 8 kDa to 12 kDa; preferably 10 kDa) and glycyrrhizin, and has unexpected technical effects.

[0144] (7) The results show that, compared with other contents of any of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid, the present invention uses 0.01wt%-0.5wt% of hydrolyzed hyaluronic acid, 0.01wt%-0.5wt% of acetylated sodium hyaluronate, and 0.01wt%-0.5wt% of silanized hyaluronic acid, which is more conducive to improving the whitening effect of the obtained cosmetic composition and has unexpected technical effects.

[0145] Experiment 4: Investigation of Anti-aging Efficacy Using the cosmetic compositions obtained in the above examples or comparative examples as test samples, the following procedures were followed for testing.

[0146] Test method: Enzyme-linked immunosorbent assay (ELISA): Matrix metalloproteinases (MMP-1) are known to cause excessive degradation of collagen and elastin, the proteins that support the skin structure in the dermis, leading to signs of aging such as wrinkles and loss of elasticity. ELISA can be used to determine the concentration of MMP-1 in cells after treatment with the analyte, thereby evaluating the efficacy of active ingredients in anti-aging cosmetics.

[0147] Evaluation of Anti-aging using a fibroblast model: Human skin fibroblasts (HSF) synthesize and secrete collagen and elastic fibers, playing a crucial role in maintaining skin elasticity and resilience. A decrease in the number of HSFs is a significant cause of wrinkles. Various methods exist for determining collagen content; ELSA can accurately quantify changes in collagen I content. Reference standard: "Evaluation of the Firming and Anti-wrinkle Efficacy of Cosmetics in Sensitive Skin: Macrophage-Fibroblast Test Method" (T / ZHCA 034-2025) Group Standard.

[0148] Cellular elastin content test: The loss of elastin is a major factor leading to skin laxity and wrinkles. The elastin content was determined using a cellular elastin colorimetric assay (TPPS) quantitative detection kit.

[0149] The positive control was a 0.1% retinol solution; the detection concentration of the test sample was 0.1 wt%. The results are shown in Table 6.

[0150] Criteria for determining anti-aging efficacy: Based on the above experimental results, the anti-aging efficacy of the test substance was comprehensively evaluated from three dimensions: collagen synthesis, elastin expression, and inhibition of collagen degradation. Collagen I production: This reflects the effect of the test substance on promoting collagen synthesis. The higher the production, the more significant the anti-aging effect.

[0151] Elastin enhancement: This reflects the effect of the test substance on improving skin elasticity; the higher the enhancement rate, the more significant the anti-aging effect.

[0152] MMP-1 inhibition rate: reflects the inhibitory effect of the test substance on collagen degradation. The higher the inhibition rate, the more significant the anti-aging effect.

[0153] Table 6: Results of Anti-aging Efficacy

[0154] in conclusion: (1) The collagen I production in the example reached more than 18.4 μg / mL (more than 78% of the positive control), and the inhibition rate of elastin and MMP-1 was significant. The mechanism is that hydrolyzed hyaluronic acid activates TLR2 receptor to promote collagen synthesis by TGF-β, acetylated sodium hyaluronate and silanized hyaluronic acid inhibit the active center of MMP-1, and glycyrrhizin blocks inflammatory damage. The four components achieve positive balance of collagen metabolism through dual-pathway regulation.

[0155] This invention scientifically combines hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid, and glycyrrhizin to fully leverage the synergistic effects of these four components. The high permeability of hydrolyzed hyaluronic acid significantly enhances the absorption efficiency of glycyrrhizin; the strong moisturizing properties of acetylated sodium hyaluronate, combined with the stability and repair capabilities of silanized hyaluronic acid, provide a solid foundation for the whitening effect of glycyrrhizin. Simultaneously, the combination of the three hyaluronic acid derivatives enhances the stability of glycyrrhizin in the formula. The synergistic effect of these four components not only significantly improves the product's moisturizing, repairing, and whitening effects but also enhances its anti-aging properties, providing an innovative solution for developing highly effective and multifunctional skincare products.

[0156] (2) The results show that, compared with products that do not contain hydrolyzed hyaluronic acid, acetylated sodium hyaluronate and silanized hyaluronic acid, or products that only contain one or two of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin, the present invention uses hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin simultaneously, which is more conducive to improving the anti-aging effect of the obtained cosmetic composition. Hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid and glycyrrhizin synergistically improve the anti-aging effect of the obtained cosmetic composition, which has unexpected technical effects.

[0157] (3) The results show that, compared with replacing any one of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, or silanized hyaluronic acid with hyaluronic acid, the hyaluronic acid derivative of the present invention, which uses hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid simultaneously, is more conducive to improving the anti-aging effect of the obtained cosmetic composition. Hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, silanized hyaluronic acid, and glycyrrhizin synergistically improve the anti-aging effect of the obtained cosmetic composition, which has unexpected technical effects.

[0158] (4) The results show that, compared with other weight-average molecular weights of hydrolyzed hyaluronic acid, the hydrolyzed hyaluronic acid with a weight-average molecular weight (selected from 1kDa-3kDa; preferably 2kDa) provided by the present invention is more conducive to improving the anti-aging effect of glycyrrhizin. It can synergistically improve the anti-aging effect of the obtained cosmetic composition with acetylated sodium hyaluronate (weight-average molecular weight selected from 8kDa-12kDa; preferably 10kDa), silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da-1.5 million Da, preferably 1.25 million Da) and glycyrrhizin, and has unexpected technical effects.

[0159] (5) The results show that, compared with other weight-average molecular weights of acetylated sodium hyaluronate, the weight-average molecular weight of acetylated sodium hyaluronate (selected from 8kDa-12kDa; preferably 10kDa) provided by the present invention is more conducive to improving the anti-aging effect of glycyrrhizin. It can synergistically improve the anti-aging effect of the obtained cosmetic composition with hydrolyzed hyaluronic acid (weight-average molecular weight selected from 1kDa-3kDa; preferably 2kDa), silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da-1.5 million Da, preferably 1.25 million Da) and glycyrrhizin, and has unexpected technical effects.

[0160] (6) The results show that, compared with other weight-average molecular weights of silanized hyaluronic acid, the weight-average molecular weight of silanized hyaluronic acid (weight-average molecular weight selected from 1 million Da to 1.5 million Da, preferably 1.25 million Da) provided by the present invention is more conducive to improving the anti-aging effect of glycyrrhizin. It can synergistically improve the anti-aging effect of the obtained cosmetic composition with hydrolyzed hyaluronic acid (weight-average molecular weight selected from 1 kDa to 3 kDa; preferably 2 kDa), acetylated sodium hyaluronate (weight-average molecular weight selected from 8 kDa to 12 kDa; preferably 10 kDa) and glycyrrhizin, and has unexpected technical effects.

[0161] (7) The results show that, compared with other contents of any one of hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, and silanized hyaluronic acid, the present invention uses 0.01wt%-0.5wt% of hydrolyzed hyaluronic acid, 0.01wt%-0.5wt% of acetylated sodium hyaluronate, and 0.01wt%-0.5wt% of silanized hyaluronic acid, which is more conducive to improving the anti-aging effect of the obtained cosmetic composition and has unexpected technical effects.

[0162] The method of this invention has been described through preferred embodiments. Those skilled in the art will readily be able to modify or appropriately alter and combine the methods and applications described herein within the scope, spirit, and context of this invention to implement and apply the technology of this invention. Those skilled in the art can refer to the content herein to appropriately improve process parameters. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included within the scope of this invention.

Claims

1. A cosmetic composition, characterized in that, Including the following active ingredients: glycyrrhizin and hyaluronic acid derivatives; The hyaluronic acid derivatives include hydrolyzed hyaluronic acid or its salts, acetylated hyaluronic acid or its salts, and silanized hyaluronic acid or its salts.

2. The cosmetic composition according to claim 1, characterized in that, The mass ratio of the hydrolyzed hyaluronic acid or its salt to the acetylated hyaluronic acid or its salt is 1:50-50:1; and / or The mass ratio of the hydrolyzed hyaluronic acid or its salt to the silanized hyaluronic acid or its salt is 1:50-50:1; and / or The mass ratio of glycyrrhizin to hyaluronic acid derivative is 1:1 to 1:60, preferably 1:3 to 1:

52.

3. The cosmetic composition according to any one of claims 1-2, wherein the weight-average molecular weight of the hydrolyzed hyaluronic acid or its salt is selected from 1 kDa to 3 kDa; preferably, the weight-average molecular weight of the hydrolyzed hyaluronic acid or its salt is 2 kDa, and the molecular weight distribution range is 1 kDa to 3 kDa; and / or The weight-average molecular weight of the acetylated hyaluronic acid or its salt is selected from 8 kDa to 12 kDa; preferably, the weight-average molecular weight of the acetylated hyaluronic acid or its salt is 10 kDa, and the molecular weight distribution range is 8 kDa to 12 kDa; and / or The weight-average molecular weight of the silanized hyaluronic acid or its salt is selected from 1,000,000 Da to 1,500,000 Da, preferably, the weight-average molecular weight of the silanized hyaluronic acid or its salt is 1,250,000 Da; the molecular weight distribution range is 1,000,000 Da to 1,500,000 Da; and / or The silanized hyaluronic acid or its salts include dimethylsilanol hyaluronic acid ester; and / or The salt of the acetylated hyaluronic acid includes sodium acetylated hyaluronic acid.

4. The cosmetic composition according to any one of claims 1-3, wherein the cosmetic composition further comprises cosmetically acceptable excipients; Optionally, the cosmetic excipients include at least one of solvents and solubilizers; Optionally, the cosmetic excipients acceptable to the cosmetic include solvents and solubilizers; Optionally, the cosmetic excipients acceptable to the cosmetic include solvents, solubilizers and other excipients; Optionally, the other excipients include: At least one of the following: emulsifier, thickener, stabilizer, moisturizer, antioxidant, preservative, skin feel modifier, pH modifier, fragrance, colorant, penetration enhancer, and sunscreen excipient; Optionally, the solubilizer includes at least one of 1,3-butanediol, 1,3-propanediol, and dipropylene glycol; Optionally, the solvent includes water.

5. The cosmetic composition according to any one of claims 1-4, wherein the content of glycyrrhizin, calculated based on the total mass of the cosmetic composition, is 0.01 wt%-0.2 wt%; and / or The content of the hydrolyzed hyaluronic acid or its salt, calculated based on the total mass of the cosmetic composition, is 0.01 wt%-0.5 wt%; and / or The content of the acetylated hyaluronic acid or its salt, calculated based on the total mass of the cosmetic composition, is 0.01 wt%-0.5 wt%; and / or The content of the silanized hyaluronic acid or its salt, calculated based on the total mass of the cosmetic composition, is 0.01 wt%-0.5 wt%; and / or The content of the solubilizer is 2wt%-20wt% based on the total mass of the cosmetic composition. The content of the solvent is 78wt.5 to 98wt%, or 78.3wt% to 97.96wt%, based on the total mass of the cosmetic composition.

6. The cosmetic composition according to any one of claims 1-4, wherein the cosmetic composition comprises a solubilizer and a solvent, the solubilizer comprising 1,3-butanediol; the solvent being water; and, based on the total mass of the cosmetic composition, the content of glycyrrhizin is 0.01wt%-0.2wt%, the content of hydrolyzed hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of acetylated hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of silanized hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of the solubilizer is 2wt%-20wt%, and the content of the solvent is 78wt%-98wt%. The cosmetic composition comprises a solubilizer and a solvent, wherein the solubilizer comprises 1,3-butanediol; the solvent is water; based on the total mass of the cosmetic composition, the content of glycyrrhizin is 0.01wt%-0.2wt%, the content of hydrolyzed hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of acetylated hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of silanized hyaluronic acid or its salt is 0.01wt%-0.5wt%, the content of the solubilizer is 2wt%-20wt%, and the content of the solvent is 78.3wt%-97.96wt%. or The cosmetic composition includes a solubilizer and a solvent, wherein the solubilizer includes 1,3-butanediol; the solvent is water; and based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.01 wt%, the content of the acetylated hyaluronic acid or its salt is 0.01 wt%, the content of the silanized hyaluronic acid or its salt is 0.01 wt%, the content of glycyrrhizin is 0.01 wt%, the content of the solubilizer is 2 wt%, and the balance is solvent; or The cosmetic composition includes a solubilizer and a solvent, wherein the solubilizer includes 1,3-butanediol; the solvent is water; and based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.01 wt%, the content of the acetylated hyaluronic acid or its salt is 0.5 wt%, the content of the silanized hyaluronic acid or its salt is 0.01 wt%, the content of glycyrrhizin is 0.01 wt%, the content of the solubilizer is 8 wt%, and the balance is solvent; or The cosmetic composition includes a solubilizer and a solvent, wherein the solubilizer includes 1,3-butanediol; the solvent is water; and based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.01 wt%, the content of the acetylated hyaluronic acid or its salt is 0.01 wt%, the content of the silanized hyaluronic acid or its salt is 0.5 wt%, the content of glycyrrhizin is 0.05 wt%, the content of the solubilizer is 8 wt%, and the balance is solvent; or The cosmetic composition includes a solubilizer and a solvent, wherein the solubilizer includes 1,3-butanediol; the solvent is water; and based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.5 wt%, the content of the acetylated hyaluronic acid or its salt is 0.5 wt%, the content of the silanized hyaluronic acid or its salt is 0.01 wt%, the content of glycyrrhizin is 0.1 wt%, the content of the solubilizer is 8 wt%, and the balance is solvent; or The cosmetic composition includes a solubilizer and a solvent, wherein the solubilizer includes 1,3-butanediol; the solvent is water; and based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.5 wt%, the content of the acetylated hyaluronic acid or its salt is 0.01 wt%, the content of the silanized hyaluronic acid or its salt is 0.5 wt%, the content of glycyrrhizin is 0.2 wt%, the content of the solubilizer is 8 wt%, and the balance is solvent; or The cosmetic composition includes a solubilizer and a solvent, wherein the solubilizer includes 1,3-butanediol; the solvent is water; and based on the total mass of the cosmetic composition, the content of the hydrolyzed hyaluronic acid or its salt is 0.5 wt%, the content of the acetylated hyaluronic acid or its salt is 0.5 wt%, the content of the silanized hyaluronic acid or its salt is 0.5 wt%, the content of glycyrrhizin is 0.2 wt%, the content of the solubilizer is 20 wt%, and the balance is solvent.

7. A method for preparing the cosmetic composition according to any one of claims 1-6, characterized in that, Includes the following steps: (1) Dissolve licorice root extract in a solubilizer to obtain solution A; (2) Mix solution A with hyaluronic acid derivative and solvent, stir until completely dissolved, filter, and obtain the cosmetic composition; or mix solution A with hyaluronic acid derivative, solvent and other excipients, stir until completely dissolved, filter, and obtain the cosmetic composition.

8. The preparation method according to claim 7, wherein step (1) comprises: Mix glycyrrhizin with a solubilizer, heat to 50℃-70℃, and stir to dissolve.

9. The use of a cosmetic composition according to any one of claims 1-6 or a cosmetic composition prepared by the preparation method according to any one of claims 7-8 in the preparation of cosmetics; Optionally, the cosmetic is used for moisturizing, repairing, whitening and / or anti-aging; Optionally, the dosage form of the cosmetic is a serum, tincture, lotion, gel, emulsion, oil, cream, or mask.

10. A cosmetic product, characterized in that, Includes the cosmetic composition according to any one of claims 1-6 or the cosmetic composition prepared by the preparation method according to any one of claims 7-8; Optionally, the cosmetic is used for moisturizing, repairing, whitening and / or anti-aging; Optionally, the dosage form of the cosmetic is a serum, tincture, lotion, gel, emulsion, oil, cream, or mask.

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