A rose essential oil, composition, use

Abstract

The application provides a rose essential oil, a composition and application, and belongs to the technical field of hepatocyte repair, and comprises the following steps: putting rose flowers and water into a distillation kettle, preheating by steam, performing vacuum distillation after reaching a predetermined temperature, and maintaining the temperature of an oil-water separator to obtain rose essential oil; a rose essential oil composition comprises the rose essential oil, and the rose essential oil accounts for 2-7% of the mass of the composition. The application also provides a rose essential oil for preparing a liver medicine, and the rose essential oil is used for repairing hepatocyte damage. The application realizes the development of natural medicine for the application of hepatocyte repair.

Description

Technical Field

[0001] This invention relates to the field of hepatocyte repair technology, specifically to a rose essential oil, a composition, and its application. Background Technology

[0002] Hepatocellular injury is closely related to the progression of liver disease. Following hepatocellular membrane damage, membrane fluidity decreases and membrane permeability increases. Decreased membrane fluidity leads to impaired activity of membrane-associated enzymes, receptors, and transport functions, inhibiting hepatocellular function. Hepatocellular injury and death (especially hepatocellular necrosis) can activate immune cells such as stellate cells, Kupffer cells, and natural killer cells, triggering liver inflammation. In chronic liver disease, this can lead to altered collagen metabolism, promoting fibrosis and cirrhosis. Therefore, hepatocellular injury is closely related to the progression of common clinical liver diseases. Therapeutic strategies that promote hepatocellular repair have a positive impact on liver disease treatment.

[0003] For the treatment of hepatocellular injury, a comprehensive treatment strategy should be adopted, which includes controlling the cause, preventing further damage, and promoting hepatocellular regeneration and repair. Drugs for repairing damaged hepatocellular injury mainly include anti-inflammatory and hepatoprotective drugs, detoxifying drugs, and antioxidants, which are usually chemical drugs. Therefore, this invention provides a novel natural drug for hepatocellular repair. Summary of the Invention

[0004] In view of this, the present invention provides a rose essential oil, a composition, and an application, realizing the application of natural medicines in the repair of liver cells.

[0005] To achieve the above objectives, the present invention provides a method for preparing rose essential oil, comprising the following steps: S1. Mix the rose petals and water evenly, and preheat with steam to reach the predetermined temperature; S2. The preheated mixture is subjected to vacuum distillation to obtain a fraction; S3. After condensing the distillate, separate the oil and water to obtain rose essential oil.

[0006] Optionally, the predetermined temperature is not lower than 98°C, the pressure in the distillation vessel during vacuum distillation is not higher than 90 kPa, the vacuum distillation time is 3~3.5 h, and the temperature of the oil-water separator is 37~45°C.

[0007] To achieve the above objectives, the present invention also provides a rose essential oil obtained by the above-mentioned method for preparing rose essential oil.

[0008] To achieve the above objectives, the present invention also provides a rose essential oil composition comprising the aforementioned rose essential oil, wherein the rose essential oil accounts for 2-7% of the composition by mass.

[0009] Optionally, the rose essential oil composition may further include excipients, including starch and sucrose.

[0010] Optionally, the rose essential oil composition may also include snow chrysanthemum polysaccharides.

[0011] Optionally, the snow chrysanthemum polysaccharide accounts for 2-5% of the mass of the composition.

[0012] Optionally, the rose essential oil composition may further include levamisole.

[0013] To achieve the above objectives, the present invention also provides an application of the above-mentioned rose essential oil in the preparation of a medicine for treating liver diseases.

[0014] Optionally, the rose essential oil is used to repair liver cell damage.

[0015] To achieve the above objectives, the present invention also provides an application of the above-mentioned rose essential oil in food.

[0016] The above-described technical solution of the present invention has at least the following beneficial effects: The natural medicine and its composition provided by this invention can be reflected by serum liver function indicators and liver immunohistochemical quantitative analysis of the positive cell rate: the natural medicine composition provided by this invention reduces serum liver function indicators ALT, AST, TBA and F4 / 80 positive cell ratio after hepatocellular damage, that is, it has the effect of repairing hepatocellular damage. Attached Figure Description

[0017] Figure 1 The following is a graph showing the serum liver function-ALT index of each group in this invention; Figure 2 Serum liver function-AST index graphs for each group in this invention; Figure 3 The following is a graph showing the serum liver function-TBA index of each group in this invention; Figure 4 The ratio of F4 / 80 positive cells in each group of this invention; Figure 5 A 40x magnified pathological section of mouse liver from the blank control group; Figure 6 A 40x magnified pathological section of the mouse liver from the model group; Figure 7 A 40x magnified section of mouse intestinal tissue from the positive control group; Figure 8 A 40x magnified section of intestinal tissue from mice in the low-dose rose essential oil group; Figure 9 A 40x magnification image of intestinal tissue sections from mice in the high-dose rose essential oil group; Figure 10Immunohistochemical image of mouse liver at 40x magnification, representing the blank control group; Figure 11 Immunohistochemical image of mouse liver at 40x magnification in the model group; Figure 12 Immunohistochemical image of mouse liver at 40x magnification, representing the positive control group; Figure 13 Immunohistochemical image of mouse liver at 40x magnification for the low-dose rose essential oil group; Figure 14 Immunohistochemical image of mouse liver at 40x magnification for the high-dose rose essential oil group. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the following will be described in conjunction with the accompanying drawings of the embodiments of the present invention. Figures 1-14 The technical solutions of the embodiments of the present invention will be clearly and completely described herein. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by those skilled in the art based on the described embodiments of the present invention are within the scope of protection of the present invention.

[0019] Example 1 A rose essential oil composition comprising rose essential oil, sucrose, and starch. The rose essential oil accounts for 4% of the composition by weight.

[0020] The method for preparing rose essential oil provided by the present invention includes: putting rose petals and water into a distillation kettle, preheating with steam, performing vacuum distillation after reaching a predetermined temperature, maintaining the temperature of the oil-water separator, and obtaining rose essential oil.

[0021] The specific method for preparing rose essential oil is as follows: The TU130 rose essential oil equipment has an exhaust valve on the distillation vessel. Before processing essential oil, open this valve first, then close the drain butterfly valve below the distillation vessel to ensure no air leakage and no water flow. Open the V-shaped door at the feed inlet and add material to the distillation vessel. The material volume should be about 2 / 3 of the total volume of the distillation vessel to be considered qualified and ready for further processing (the specific weight is approximately 500 kg).

[0022] 2. Add water during distillation The water volume for the TU130 essential oil dispenser should be controlled at around 200 kg. It is best to install a water meter on the water supply pipe. If there is no water meter, the water volume should generally be determined by filling the pipe 10 cm above the outlet pipe.

[0023] 3. Heating distillation 3.1 Before introducing steam, check whether the feed port of the distillation vessel is closed and whether the exhaust valve is open. Cold air needs to be vented. When adding steam, first add 1 / 3 of the total air volume of the pipeline through the valve, and preheat for about 40 minutes.

[0024] 3.2 After timing for 40 minutes, increase the steam flow by 1 / 3. When the exhaust valve on the distillation vessel begins to release steam, start recording the distillation time, and close the exhaust valve on the distillation vessel. Begin adjusting the valve to maintain 2 / 3 of the steam flow for 2.5 hours, referring to the temperature gauge on the distillation vessel. Once the steam temperature reaches the predetermined temperature, begin normal distillation. The predetermined temperature is above 98 degrees Celsius, and it typically reaches the predetermined temperature after 1.0 hour of heating.

[0025] 3.3 The gas supply pressure of the pressure pack should be above 2 kg, the gas supply from the boiler should be no less than 3 kg, and the pressure inside the distillation vessel should be below 0.9 kg (i.e., ≤90 kPa), which is beneficial for the extraction of rose aromatics. Reduced pressure distillation is adopted. When the distillation time exceeds 3.0 hours, the gas pressure is adjusted to the maximum, and the time is maintained for 40 minutes. The entire distillation process, from flower loading to distillation completion, takes 4 hours.

[0026] 4. Condensation When steam is discharged from the exhaust valve on the distillation vessel, begin to open the water supply valve on the condenser, opening it to approximately half capacity. Maintain the temperature gauge reading on the oil-water separator between 37-45°C. If the temperature is too high, increase the water flow; if the temperature is below 37°C, decrease the water flow. When the distillation time is complete (based on the recorded start time), close the gas supply valve, but do not close the cooling water supply valve. Generally, close the cooling water 10 minutes later.

[0027] 5. Operation of the oil-water separator The return water pipe on the oil-water separator has no return water for the first 40 minutes of distillation. When return water begins to flow, open the return valve to allow the return pipe to automatically circulate the water. The circulated rose hydrosol returns to the distillation tower. After 2.5 hours of distillation, close the return valve, and rose hydrosol will begin to flow out. At this point, the oil-water separator is almost full of rose hydrosol. You can then open the valve at the bottom of the oil-water separator to start releasing the rose hydrosol. Generally, leave 1 / 3 of the rose hydrosol at the bottom of the oil-water separator. Remember not to release all the rose hydrosol at once to prevent the rose essential oil from flowing out.

[0028] 6. Discharge Before discharging, first close the gas supply valve of the steam separator, then close the gas supply valve of the distillation kettle, close the condensate supply valve, open the air vent valve on top of the distillation kettle, and open the water supply valve to the distillation kettle to allow cold water to enter the distillation kettle. Once everything is ready, open the drain valve at the bottom of the distillation kettle to start discharging. The discharging time is generally 15 minutes. While discharging, rinse the distillation tank and distillation tower with cold water. Generally, from feeding to discharging, it takes about 5.5 hours. The entire distillation is then complete, and the next cycle begins.

[0029] 7. Extracting rose essential oil When there is too much rose essential oil in the oil cup on the oil-water separator, you can start extracting the rose essential oil. When extracting the rose essential oil, operate the reflux valve with one hand to let the reflux water enter the oil-water separator, causing the rose essential oil level to rise. When it rises to a certain level, the rose essential oil will start to flow out from the drain pipe. Place a larger container under the bottle containing the rose essential oil to prevent the rose essential oil from overflowing and being wasted.

[0030] Example 2 Compared with Example 1, the only difference is that in this example, rose essential oil accounts for 6% of the composition by mass.

[0031] Example 3 Compared with Example 1, the only difference is that in this example, rose essential oil accounts for 2% of the mass of the rose essential oil composition.

[0032] Example 4 Compared with Example 1, the only difference is that in this example, rose essential oil accounts for 7% of the mass of the rose essential oil composition.

[0033] Example 5 A rose essential oil composition comprising rose essential oil, starch, sucrose, and snow chrysanthemum polysaccharide. The rose essential oil accounts for 5% of the composition by weight, and the snow chrysanthemum polysaccharide accounts for 2% of the composition by weight.

[0034] Example 6 Compared with Example 5, the only difference is that the snow chrysanthemum polysaccharide accounts for 5% of the composition mass.

[0035] Example 7 A rose essential oil composition comprising rose essential oil and levamisole, sucrose, and starch.

[0036] Example 8 A liquid food product comprising a rose essential oil composition.

[0037] Example 9 A non-liquid food product comprising a rose essential oil composition.

[0038] Comparative Example 1 Compared to Example 1, the only difference is that the rose essential oil in the rose essential oil composition is replaced with levamisole.

[0039] Mouse experiments with immune-enhancing compositions I. Experimental Methods (I) Model Construction SPF-grade male BALB / c mice (8 weeks old, weighing 20±2g), 10 mice per group, 6 groups in total: 1. Control group: normal saline administered by gavage (0.2 mL / d) + normal saline administered by intraperitoneal injection; 2. Model group (CTX): normal saline by gavage + cyclophosphamide (150mg / kg / day, intraperitoneal injection, for 3 consecutive days); 3. Positive control group (levamisole): Levamisole (5 mg / kg / d) administered by gavage + cyclophosphamide; 4. Low-dose rose essential oil group: Rose essential oil (100mg / kg / d) administered by gavage + cyclophosphamide; 5. High-dose rose essential oil group: Rose essential oil (200mg / kg / d) was administered by gavage + cyclophosphamide.

[0040] Cyclophosphamide is hepatotoxic, manifested by elevated transaminase and total bile acid levels.

[0041] (II) Intervention Cycle 1. Seven-week-old mice were acclimatized for one week. Cyclophosphamide was administered via gavage starting on day 4 after injection and continued for 28 days.

[0042] 2. After the intervention, the patient was kept fasting for 12 hours, anesthetized with 10% chloral hydrate, blood was drawn and the patient was euthanized, and the tissue was immediately separated.

[0043] (III) Testing Indicators Liver function tests are used to measure alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bile acids (TBA) to assess the damage and repair status of liver cells.

[0044] The proportion of macrophages was determined by quantitative analysis of the F4 / 80 positive cell rate using liver immunohistochemistry.

[0045] The condition of the liver is determined by examining liver pathological sections and liver immunohistochemical images.

[0046] II. Experimental Results (a) ALT, AST, and TBA indicators Figure 1 The above graphs show the serum liver function-ALT indexes for each group. Figure 2 Serum liver function-AST index graphs for the above groups; Figure 3 The above graphs show the serum liver function-TBA index for each group.

[0047] Depend on Figure 1 It can be seen that, with the blank control group as the baseline, the ALT index in the model group was significantly increased, indicating that the hepatotoxicity of cyclophosphamide damaged hepatocytes, resulting in an increase in the alanine aminotransferase (ALT) index; the ALT index in the positive control group was lower than that in the model group, indicating that levamisole can reduce the ALT index; the ALT index in the low-dose rose essential oil group was higher than that in the positive control group but lower than that in the model group, while the ALT index in the high-dose rose essential oil group was slightly lower than that in the positive control group, indicating that rose essential oil has the effect of reducing the ALT index, and the effect of high-dose rose essential oil is stronger than that of levamisole.

[0048] Depend on Figure 2 It can be seen that, with the blank control group as the baseline, the aspartate aminotransferase (AST) level in the model group was significantly increased, indicating that the hepatotoxicity of cyclophosphamide damaged hepatocytes, resulting in an increase in AST. The AST level in the positive control group was lower than that in the model control group, indicating that levamisole can reduce the AST level. The AST level in the low-dose rose essential oil group was lower than that in the model group, similar to the positive control group. The AST level in the high-dose rose essential oil group was lower than that in the low-dose rose essential oil control group, indicating that rose essential oil has the effect of reducing the AST level, and the effect of high-dose rose essential oil is stronger than that of levamisole.

[0049] Depend on Figure 3 It can be seen that, with the blank control group as the baseline, the total bile acid (TBA) index in the model group was significantly increased, indicating that the hepatotoxicity of cyclophosphamide damaged hepatocytes, resulting in an increase in the TBA index; the TBA index in the positive control group was lower than that in the model group, indicating that levamisole can reduce the TBA index; the ALT index in the low-dose rose essential oil group was lower than that in the model group, similar to the positive control group; the TBA index in the high-dose rose essential oil group was lower than that in the low-dose rose essential oil control group, indicating that rose essential oil has the effect of reducing the TBA index, and the effect of high-dose rose essential oil is stronger than that of levamisole.

[0050] In summary, the rose essential oil provided by this invention promotes liver cell repair and reduces the increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bile acid (TBA) levels caused by liver damage.

[0051] (ii) F4 / 80 positive cell rate Figure 4 The ratio of F4 / 80 positive cells in each of the above groups.

[0052] Depend on Figure 4 It can be seen that, with the blank control group as the baseline, the F4 / 80 positive cell rate in the model group was significantly increased, indicating that the hepatotoxicity of cyclophosphamide damaged hepatocytes, resulting in an increase in the F4 / 80 positive cell rate. The F4 / 80 positive cell rate in the positive control group was lower than that in the model group, indicating that levamisole can reduce the F4 / 80 positive cell rate. The F4 / 80 positive cell rate in the low-dose rose essential oil group was lower than that in the model group but higher than that in the positive control group. The F4 / 80 positive cell rate in the high-dose rose essential oil group was lower than that in the low-dose rose essential oil group but slightly higher than that in the positive control group. This indicates that rose essential oil has the effect of reducing the F4 / 80 positive cell rate, but the effect of high-dose rose essential oil is weaker than that of levamisole.

[0053] In summary, the rose essential oil provided by this invention promotes liver cell repair and reduces the increase in the F4 / 80 positive cell ratio after liver damage.

[0054] (III) Liver condition Figure 5 A 40x magnified pathological section of mouse liver from the blank control group; Figure 6 A 40x magnified image of the intestinal tissue section of the model group mice; Figure 7 A 40x magnified section of mouse intestinal tissue from the positive control group; Figure 8 A 40x magnified section of intestinal tissue from mice in the low-dose rose essential oil group; Figure 9 This is a 40x magnified image of the intestinal tissue of mice in the high-dose rose essential oil group.

[0055] Figure 10 Immunohistochemical image of mouse liver at 40x magnification, representing the blank control group; Figure 11 Immunohistochemical image of mouse liver at 40x magnification in the model group; Figure 12 Immunohistochemical image of mouse liver at 40x magnification, representing the positive control group; Figure 13 Immunohistochemical image of mouse liver at 40x magnification for the low-dose rose essential oil group; Figure 14 Immunohistochemical image of mouse liver at 40x magnification for the high-dose rose essential oil group.

[0056] Depend on Figure 10 It is known that under normal conditions, the number of Kupffer cells in the liver of mice is normal. Kupffer cells are responsible for monitoring and clearing foreign substances and maintaining immune homeostasis.

[0057] Depend on Figure 11 It is known that the hepatotoxicity of cyclophosphamide leads to hepatocyte damage and death. These damage signals strongly activate Kupffer cells, causing them to proliferate and become activated, and recruit circulating monocytes to transform into macrophages, collectively resulting in a significant increase in the number of F4 / 80 positive cells in the liver. This is direct evidence of an exacerbated liver inflammatory response.

[0058] Depend on Figure 12 It is known that effective positive control drugs (such as levamisole) can significantly reduce the F4 / 80 positive cell rate and bring it back to normal levels by alleviating liver damage and / or directly inhibiting the excessive activation of macrophages.

[0059] Depend on Figure 13 It is known that the low-dose rose essential oil provided by this invention has certain anti-inflammatory and macrophage activation inhibitory effects, but the effects are not yet complete.

[0060] Depend on Figure 14 It is known that the high-dose rose essential oil provided by the present invention can effectively inhibit the excessive activation of macrophages, thereby significantly reducing the rate of F4 / 80 positive cells.

[0061] The above are preferred embodiments of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for preparing rose essential oil, characterized in that, Includes the following steps: S1. Mix the rose petals and water evenly, and preheat with steam to reach the predetermined temperature; S2. The preheated mixture is subjected to vacuum distillation to obtain a fraction; S3. After condensing the distillate, separate the oil and water to obtain rose essential oil.

2. The method for preparing rose essential oil according to claim 1, characterized in that, The predetermined temperature is not lower than 98℃, the pressure in the distillation vessel during vacuum distillation is not higher than 90 kPa, the vacuum distillation time is 3~3.5h, and the temperature of the oil-water separator is 37~45℃.

3. Rose essential oil obtained by the method for preparing rose essential oil according to any one of claims 1 to 2.

4. A rose essential oil composition, characterized in that, The composition includes the rose essential oil as described in claim 3, wherein the rose essential oil accounts for 2 to 7% of the mass of the composition.

5. The rose essential oil composition according to claim 4, characterized in that, The composition also includes excipients, which include starch and sucrose.

6. The rose essential oil composition according to claim 5, characterized in that, The composition also includes snow chrysanthemum polysaccharide.

7. The rose essential oil composition according to claim 6, characterized in that, The snow chrysanthemum polysaccharide accounts for 2-5% of the mass of the composition.

8. The rose essential oil composition according to claim 6, characterized in that, The composition also includes levamisole.

9. The use of the rose essential oil as described in claim 3 in the preparation of a medicament for treating liver diseases.

10. An application of the rose essential oil as described in claim 3 in the preparation of food.

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