A glucose oxidase preparation device

Abstract

The application relates to the technical field of glucose oxidase preparation, in particular to a glucose oxidase preparation device, which comprises a salting-out box, the upper end of the salting-out box is fixedly connected with a feeding box, the salting-out box is communicated with the feeding box, the upper end of the feeding box is fixedly connected with a storage hopper, the storage hopper is communicated with the feeding box, a rotating ring is movably connected in the feeding box, and connecting cavities are formed in the outer side of the rotating ring; after the rotating ring rotates and the curved rod is in contact with the arc-shaped guide plate, the curved rod moves to push the gas into the connecting cavities, so that the telescopic stirring mechanism is extended, the connecting ring is rotated and drives the telescopic stirring mechanism to rotate, the salting-out agent stored in the connecting ring is broken, the broken salting-out agent is heated and dried by the arc-shaped heating plate, when the connecting ring rotates to the lower end, the salting-out agent falls into the mixed aluminum cylinder to carry out a mixing reaction with the crude enzyme liquid, and the salting-out agent is prevented from being blocked and falling.

Description

Technical Field

[0001] This invention relates to the field of glucose oxidase preparation technology, specifically to a glucose oxidase preparation apparatus. Background Technology

[0002] In recent years, with the rapid development of modern biomedicine, the application prospects of glucose oxidase are being continuously expanded to more cutting-edge medical fields. Especially against the backdrop of utilizing biotechnology and DNA technology in medical activities, its value is increasingly prominent. For example, in in vitro diagnostics based on enzyme-linked immunosorbent assay (ELISA) and PCR-derived technologies, glucose oxidase is often used as a key signal amplification enzyme for highly sensitive detection of disease biomarkers. In genetically engineered immunocellular therapies or targeted drug delivery systems, this enzyme can also be designed as a "bioswitch" to activate specific prodrugs or to regulate the tumor microenvironment. These high-end applications place almost stringent requirements on the purity, specific activity, stability, and batch-to-batch consistency of glucose oxidase preparations.

[0003] For example, Chinese patent CN119020162B discloses a glucose oxidase preparation device, including a salting-out chamber. A mixing aluminum cylinder is movably installed on the upper side of the inside of the salting-out chamber via a bearing seat. A central shaft is movably installed in the middle of the salting-out chamber. Stirring rods are fixedly connected to both sides of the middle of the central shaft. Magnetic rods are fixedly installed on both sides of the inside of the salting-out chamber. Feeding chambers are fixedly connected to both sides of the inside of the shell, and the bottom ends of the feeding chambers are connected to the inside of the mixing aluminum cylinder.

[0004] In the aforementioned patent, when the long rod moves upward, the blocking block seals the feeding channel, and the stop block also releases the obstruction of the feeding branch pipe, allowing the salting-out agent to enter the feeding chamber. The continuous feeding of the salting-out agent is achieved by the alternating up and down operation of the two long rods. However, the above solution has the following shortcomings: When preparing glucose oxidase using biotechnology and DNA technology in medical activities, if the salting-out agent becomes damp and clumps, the salting-out agent clumps entering the feeding channel will cause blockage, preventing the salting-out agent from continuously entering the mixing aluminum cylinder. Therefore, we have introduced a glucose oxidase preparation device. Summary of the Invention

[0005] The purpose of this invention is to provide a glucose oxidase preparation apparatus to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A glucose oxidase preparation device includes a salting-out tank, a conveying tank fixedly connected to the upper end of the salting-out tank, the salting-out tank and the conveying tank being connected, a storage hopper fixedly connected to the upper end of the conveying tank, the storage hopper being connected to the conveying tank, a rotating ring movably connected inside the conveying tank, connecting cavities being formed around the outer perimeter of the rotating ring, connecting rings movably connected within the connecting cavities, one end of the connecting ring extending into the conveying tank, several toothed grooves being formed on the outer side of the connecting ring, and several telescopic stirring mechanisms being provided inside the connecting ring. By rotating the connecting ring, the extended telescopic stirring mechanisms are driven to rotate, thus stirring the salting-out agent. The toothed grooves mesh with a first toothed ring, and the first toothed ring is fixedly connected to the inner side of the conveying tank.

[0008] The connecting ring is provided with a clearance mechanism on both sides, which is located inside the rotating ring. The clearance mechanism allows the gas entering the connecting cavity to make way. The lower end of the connecting cavity is connected to two gas storage chambers. The gas storage chambers are located inside the rotating ring. A curved rod is slidably connected inside the gas storage chamber. The end of the curved rod away from the gas storage chamber extends into the material conveying box. An arc-shaped guide plate is provided inside the material conveying box. A piston rod is movably connected inside the connecting cavity. The lower end of the piston rod extends into the material conveying box and is connected to an adjustment mechanism. The adjustment mechanism is located inside the material conveying box. The height of the piston rod in the connecting cavity is adjusted by the adjustment mechanism. A limit spring is fixedly connected inside the gas storage chamber. The other end of the limit spring is fixedly connected to the curved rod.

[0009] A turntable is movably connected to the bottom of the inner side of the salting-out tank. The upper end of the turntable is clamped by two clamping mechanisms to hold a mixing aluminum cylinder. The lower end of the mixing aluminum cylinder is arc-shaped. The clamping mechanism is located on the upper end of the turntable. A stirring mechanism is provided inside the mixing aluminum cylinder. The lower end of the stirring mechanism is connected to a transmission mechanism. The transmission mechanism is located inside the salting-out tank. An arc-shaped heating plate is fixedly connected to the inner side of the conveying box.

[0010] Preferably, the telescopic stirring mechanism includes a T-shaped tube, which is slidably connected to the through cavity, which is opened in the connecting ring. A first spring is sleeved on the outside of the T-shaped tube, with one end of the first spring fixedly connected to the T-shaped tube and the other end fixedly connected to the through cavity.

[0011] Preferably, a T-shaped rod is movably connected inside the T-shaped tube, and a second spring is sleeved on the outside of the T-shaped rod. One end of the second spring is fixedly connected to the T-shaped rod, and the other end is fixedly connected to the inside of the T-shaped tube.

[0012] Preferably, the clearance mechanism includes a push plate that slides within a clearance cavity, the clearance cavity being located within a rotating ring and communicating with a connecting cavity. Two third springs are fixedly connected to one side of the push plate, and the end of each third spring furthest from the push plate is fixedly connected to the clearance cavity.

[0013] Preferably, the adjusting mechanism includes an external threaded rod, which is screwed into the material conveying box, with both ends of the external threaded rod extending into the external environment. A conical ring is movably connected to the outside of the external threaded rod, and T-slots are formed around the outer perimeter of the conical ring. The lower end of the piston rod slides into the T-slots, and two internal threaded rings are screwed to the outside of the external threaded rod.

[0014] Preferably, the clamping mechanism includes a clamping rod, the lower end of which is slidably connected to a T-shaped guide groove, the T-shaped guide groove being opened at the upper end of the turntable, a support spring being fixedly connected in the T-shaped guide groove, the other end of which is fixedly connected to the clamping rod, and one end of the clamping rod being engaged in a snap-fit ​​hole, the snap-fit ​​hole being opened on the outside of the mixed aluminum cylinder.

[0015] Preferably, the stirring mechanism includes a connecting shaft, which is movably connected to the lower end of the mixing aluminum cylinder. The upper end of the connecting shaft extends into the inner side of the mixing aluminum cylinder and is fixedly connected to two stirring rods. A square insertion hole is provided at the lower end of the connecting shaft, and the lower end of the square insertion hole is arc-shaped.

[0016] Preferably, the transmission mechanism includes a T-shaped toothed column, which is movably connected to a T-shaped toothed tube. The T-shaped toothed tube is movably connected to the inside of the salting-out tank. The upper end of the T-shaped toothed tube is fixedly connected to the lower end of the turntable. The upper end of the T-shaped toothed column passes through the turntable. A transmission gear meshes between the T-shaped toothed column and the T-shaped toothed tube. The transmission gear is fixedly connected to the output end of the transmission motor. The transmission motor is fixedly connected to the salting-out tank. A sliding cavity is provided at the upper end of the T-shaped toothed column. A limit rod slides in the sliding cavity. The outer side of the limit rod is square. The upper end of the limit rod is inserted into a square socket. A return spring is fixedly connected in the sliding cavity. The upper end of the return spring is fixedly connected to the lower end of the limit rod.

[0017] Preferably, two vibrators are fixedly connected inside the storage hopper, a door is movably connected to one side of the salting-out box, a connecting gear ring is fixedly connected to one side of the rotating ring, the inner side of the connecting gear ring meshes with a connecting gear, and a connecting motor is fixedly connected to the outside of the conveying box, the input end of the connecting motor extends into the conveying box and is fixedly connected to the connecting gear.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: when the rotating ring drives the connecting ring to rotate to the upper end, the salting-out agent in the storage hopper will enter the connecting ring for storage. As the rotating ring rotates, the curved rod contacts the arc-shaped guide plate, and the curved rod moves to push the gas into the connecting cavity, thereby causing the telescopic stirring mechanism to extend. By rotating the connecting ring and driving the telescopic stirring mechanism to rotate, the salting-out agent stored in the connecting ring is broken up. The broken salting-out agent is heated and dried by the arc-shaped heating plate. When the connecting ring rotates to the lower end, the salting-out agent will fall into the mixing aluminum cylinder to mix and react with the crude enzyme solution, preventing the salting-out agent from clumping and being unable to fall. Attached Figure Description

[0019] Figure 1 This is a three-dimensional cross-sectional view of the present invention;

[0020] Figure 2 This is a three-dimensional structural diagram of the present invention;

[0021] Figure 3 This is a three-dimensional structural diagram of the mixed aluminum cylinder in the removed state according to the present invention;

[0022] Figure 4 This is a schematic diagram of the three-dimensional structure of the rotating ring of the present invention;

[0023] Figure 5 This is a three-dimensional structural diagram of the external threaded rod position of the present invention;

[0024] Figure 6 For the present invention Figure 1 Enlarged structural diagram at point A in the middle;

[0025] Figure 7 For the present invention Figure 1 Enlarged structural diagram at point B;

[0026] Figure 8 This is a schematic diagram of the three-dimensional cross-sectional structure of the rotating ring of the present invention;

[0027] Figure 9 For the present invention Figure 8 Enlarged structural diagram at point C;

[0028] Figure 10 For the present invention Figure 8 Enlarged structural diagram at point D;

[0029] Figure 11 This is a schematic diagram of the three-dimensional structure of the connecting ring of the present invention;

[0030] Figure 12 This is a three-dimensional structural diagram illustrating the positional relationship between the connecting cavity and the gas storage cavity of the present invention;

[0031] Figure 13This is a three-dimensional structural diagram of the T-tube and T-rod of the present invention in a separated state;

[0032] Figure 14 This is a three-dimensional structural diagram illustrating the connection relationship between the conical ring and the externally threaded rod of the present invention;

[0033] Figure 15 This is a three-dimensional structural diagram of the conical ring and the externally threaded rod in the separated state of the present invention;

[0034] Figure 16 This is a three-dimensional cross-sectional view of the square insertion hole and the limiting rod of the present invention in a separated state;

[0035] Figure 17 This is a three-dimensional cross-sectional structural diagram of the connection relationship between the square connecting gear and the connecting toothed ring of the present invention.

[0036] In the diagram: 1. Salting-out tank; 2. Feeding box; 3. External threaded rod; 4. Storage hopper; 5. First spring; 6. Box door; 7. Stirring rod; 8. Piston rod; 9. Arc-shaped guide plate; 10. Drive motor; 11. Rotary ring; 12. Drive gear; 13. T-shaped toothed tube; 14. Second spring; 15. Vibrator; 16. Turntable; 17. Mixed aluminum cylinder; 18. Snap-fit ​​hole; 19. Connecting toothed ring; 20. Clamping rod; 21. T-shaped guide groove; 22. Support spring; 23. Connecting shaft; 24. Square insertion hole; 25. Limiting rod; 26. Sliding cavity; 27. Return spring; 28. T-shaped toothed column; 29. ​​Through cavity; 30. T-shaped tube; 31. T-shaped rod; 32. Connecting cavity; 33. Connecting ring; 34. Connecting motor; 35. Curved rod; 36. Conical ring; 37. T-shaped groove; 38. Toothed groove; 39. Internal threaded ring; 40. Connecting gear; 41. Relief cavity; 42. Push plate; 43. Third spring; 44. Air storage cavity; 45. Limiting spring; 46. First toothed ring; 47. Arc-shaped heating plate. Detailed Implementation

[0037] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] Please see Figure 1-17 The present invention provides a technical solution:

[0039] Example 1:

[0040] A glucose oxidase preparation apparatus includes a salting-out tank 1, with a conveying tank 2 fixedly connected to the upper end of the salting-out tank 1, and the salting-out tank 1 and the conveying tank 2 being in communication. A storage hopper 4 is fixedly connected to the upper end of the conveying tank 2. When preparing glucose oxidase using biotechnology and DNA technology in medical activities, the storage hopper 4 is used to store the salting-out agent, which can be ammonium sulfate solid granules. A lid is snapped onto the upper end of the storage hopper 4. The storage hopper 4 is in communication with the conveying tank 2. A rotating ring 11 is movably connected inside the conveying tank 2. The outer side of the rotating ring 11 is tightly fitted to the inner wall of the conveying tank 2. Connecting cavities 32 are formed around the outer perimeter of the rotating ring 11. A connecting ring 33 is movably connected inside the cavity 32. One end of the connecting ring 33 extends into the conveying box 2. Several toothed grooves 38 are provided on the outer side of the connecting ring 33. Several telescopic stirring mechanisms are provided inside the connecting ring 33. By rotating the connecting ring 33, the extended telescopic stirring mechanisms are driven to rotate, thus stirring the salting-out agent. The toothed grooves 38 mesh with the first toothed ring 46. The first toothed ring 46 is fixedly connected to the inner side of the conveying box 2. During the rotation of the rotating ring 11, since the toothed grooves 38 on the outer side of the connecting ring 33 mesh with the first toothed ring 46, the connecting ring 33 will start to rotate when the rotating ring 11 drives the connecting ring 33 to move.

[0041] The connecting ring 33 is provided with clearance mechanisms on both sides, which are located inside the rotating ring 11. These clearance mechanisms allow gas entering the connecting cavity 32 to pass through. The lower end of the connecting cavity 32 is connected to two gas storage chambers 44, which are located inside the rotating ring 11. A curved rod 35 slides within the gas storage chamber 44. The end of the curved rod 35 away from the gas storage chamber 44 extends into the conveying box 2. A roller is movably connected to the end of the curved rod 35 that contacts the arc-shaped guide plate 9. The conveying box 2 is equipped with an arc-shaped guide plate 9. Figure 4 , 8 As shown, during the rotation of the rotating ring 11, the curved rod 35 will come into contact with the arc-shaped guide plate 9. As the rotating ring 11 continues to rotate, the curved rod 35 will be guided upward by the arc-shaped guide plate 9. At this time, the gas in the gas storage chamber 44 will be pushed into the connecting chamber 32 outside the connecting ring 33.

[0042] A piston rod 8 is movably connected inside the connecting cavity 32. The lower end of the piston rod 8 extends into the material conveying box 2 and is connected to the adjusting mechanism. The adjusting mechanism is located inside the material conveying box 2. The height of the piston rod 8 in the connecting cavity 32 is adjusted by the adjusting mechanism. A limit spring 45 is fixedly connected inside the air storage cavity 44. The other end of the limit spring 45 is fixedly connected to the curved rod 35. When the curved rod 35 is no longer in contact with the arc-shaped guide plate 9, the curved rod 35 will return to its initial position under the elastic force of the limit spring 45.

[0043] A turntable 16 is movably connected to the bottom of the inner side of the salting-out tank 1. The upper end of the turntable 16 is clamped by two clamping mechanisms to hold the mixing aluminum cylinder 17. The lower end of the mixing aluminum cylinder 17 is arc-shaped. The clamping mechanism is located on the upper end of the turntable 16. A stirring mechanism is provided inside the mixing aluminum cylinder 17. The lower end of the stirring mechanism is connected to the transmission mechanism. The transmission mechanism is located inside the salting-out tank 1. An arc-shaped heating plate 47 is fixedly connected to the inner side of the conveying box 2. The arc-shaped heating plate 47 can be selected in appropriate size and model during use.

[0044] Example 2:

[0045] Based on Example 1, in order to allow the agglomerated salting-out agent to enter the connecting ring 33 through the connecting cavity 32, the telescopic stirring mechanism includes a T-shaped tube 30, which is slidably connected to the through cavity 29, which is opened within the connecting ring 33. A first spring 5 is sleeved on the outside of the T-shaped tube 30, with one end of the first spring 5 fixedly connected to the T-shaped tube 30 and the other end fixedly connected to the through cavity 29. A T-shaped rod 31 is movably connected inside the T-shaped tube 30, and a second spring is sleeved on the outside of the T-shaped rod 31. Spring 14, one end of the second spring 14 is fixedly connected to the T-shaped rod 31, and the other end is fixedly connected to the inside of the T-shaped tube 30. As the rotating ring 11 continues to rotate, the curved rod 35 will no longer be in contact with the arc-shaped guide plate 9. At this time, under the elastic force of the first spring 5 and the second spring 14, the T-shaped tube 30 and the T-shaped rod 31 retract to the initial position to prevent the T-shaped tube 30 and the T-shaped rod 31 from extending out, so that the clumped salting agent cannot enter the connecting ring 33 for storage through the connecting cavity 32;

[0046] like Figure 10 As shown, the clearance mechanism includes a push plate 42, which is slidably connected to the clearance cavity 41. The outer side of the push plate 42 is tightly fitted to the clearance cavity 41. The clearance cavity 41 is opened in the rotating ring 11 and is connected to the connecting cavity 32. Two third springs 43 are fixedly connected to one side of the push plate 42. The end of the third spring 43 away from the push plate 42 is fixedly connected to the clearance cavity 41. When the piston rod 8 moves upward to block several T-shaped tubes 30, after some T-shaped tubes 30 and T-shaped rods 31 extend, the excess gas will push the push plate 42 to move, thereby allowing the gas to enter the clearance cavity 41, ensuring that the curved rod 35 can always move along the arc-shaped guide plate 9.

[0047] like Figure 14 , 15As shown, the adjusting mechanism includes an external threaded rod 3, which is screwed into the feed box 2, with both ends of the external threaded rod 3 extending into the external environment. A conical ring 36 is movably connected to the outside of the external threaded rod 3, and two sliding rings are fixedly connected to the inside of the conical ring 36. The sliding rings slide in the sliding grooves provided on the outside of the external threaded rod 3. With this structure, the conical ring 36 can rotate with the piston rod 8 when it rotates. T-grooves 37 are provided around the outside of the conical ring 36, and the lower end of the piston rod 8 slides in the T-grooves 37. Two internal threaded rings 39 are screwed to the outside of the external threaded rod 3.

[0048] The clamping mechanism includes a clamping rod 20, the lower end of which slides into a T-shaped guide groove 21. The T-shaped guide groove 21 is located on the upper end of the turntable 16. A support spring 22 is fixedly connected inside the T-shaped guide groove 21. The other end of the support spring 22 is fixedly connected to the clamping rod 20. One end of the clamping rod 20 is engaged in a snap-fit ​​hole 18, which is located on the outside of the mixed aluminum cylinder 17. By pushing the two clamping rods 20 outward, one end of the clamping rod 20 can be disengaged from the snap-fit ​​hole 18. When the pushing of the clamping rod 20 is released, the clamping rod 20 returns to its initial position under the elastic force of the support spring 22.

[0049] The stirring mechanism includes a connecting shaft 23, which is movably connected to the lower end of the mixing aluminum cylinder 17. The upper end of the connecting shaft 23 extends into the inner side of the mixing aluminum cylinder 17 and is fixedly connected to two stirring rods 7. A square insertion hole 24 is provided at the lower end of the connecting shaft 23. The lower end of the square insertion hole 24 is arc-shaped. When the mixing aluminum cylinder 17 is pulled outward, the limiting rod 25 is squeezed into the sliding cavity 26 because the lower end of the square insertion hole 24 is arc-shaped. The transmission mechanism includes a T-shaped toothed column 28, which is movably connected to a T-shaped toothed tube 13. The T-shaped toothed tube 13 is movably connected to the salting-out tank 1. Inside, the upper end of the T-shaped toothed tube 13 is fixedly connected to the lower end of the turntable 16. The upper end of the T-shaped toothed column 28 passes through the turntable 16. A transmission gear 12 meshes between the T-shaped toothed column 28 and the T-shaped toothed tube 13. The transmission gear 12 is fixedly connected to the output end of the transmission motor 10. The transmission motor 10 is fixedly connected to the salting-out box 1. A sliding cavity 26 is opened at the upper end of the T-shaped toothed column 28. A limit rod 25 slides in the sliding cavity 26. The outer side of the limit rod 25 is square. The upper end of the limit rod 25 is inserted into the square insertion hole 24. A return spring 27 is fixedly connected in the sliding cavity 26. The upper end of the return spring 27 is fixedly connected to the lower end of the limit rod 25.

[0050] Two vibrators 15 are fixedly connected inside the storage hopper 4. The vibrators 15 can be selected in appropriate size and model during use. A door 6 is movably connected to one side of the salt precipitation box 1. A connecting gear ring 19 is fixedly connected to one side of the rotating ring 11. The inner side of the connecting gear ring 19 meshes with the connecting gear 40. A connecting motor 34 is fixedly connected to the outside of the conveying box 2. The input end of the connecting motor 34 extends into the conveying box 2 and is fixedly connected to the connecting gear 40.

[0051] Working principle: During use, when preparing glucose oxidase for medical activities using biotechnology and DNA technology, crude enzyme solution is added to the mixing aluminum cylinder 17. After addition, the door 6 is opened, and the mixing aluminum cylinder 17 containing crude enzyme solution is pushed into the salting-out tank 1 along the turntable 16. Because the bottom of the mixing aluminum cylinder 17 is arc-shaped and the outer side of the mixing aluminum cylinder 17 is circular, the limiting rod 25 is squeezed and moved into the sliding cavity 26 during the pushing of the mixing aluminum cylinder 17. At this time, the return spring 27 is compressed. When the square insertion hole 24 moves to the position of the limiting rod 25, the limiting rod 25 will be inserted into the square insertion hole 24 under the elastic force of the return spring 27. At the same time, when the mixing aluminum cylinder 17 moves, the clamping rod 20 will be squeezed and moved along the T-shaped guide groove 21. At this time, the support spring 22 is compressed. When the snap-fit ​​hole 18 located on the outside of the mixing aluminum cylinder 17 moves to the position of the clamping rod 20, the clamping rod 20 will be inserted into the snap-fit ​​hole 18 under the elastic force of the support spring 22, so that the mixing aluminum cylinder 17 is limited.

[0052] Open the lid at the top of the storage hopper 4 and put the salting-out agent into the storage hopper 4. After putting it in, reinstall the lid at the top of the storage hopper 4. Adjust the position of the piston rod 8 according to the amount of salting-out agent to be added to the mixing aluminum cylinder 17 each time. Specifically, loosen the two internal threaded rings 39 so that the external threaded rod 3 can rotate. When the external threaded rod 3 is rotated and moves inward, it will drive the conical ring 36 to move inward. Since the lower end of the piston rod 8 slides in the T-groove 37, when the conical ring 36 moves, the piston rod 8 will move from the lower part to the higher part of the T-groove 37. At this time, the piston rod 8 will move upward to reduce the amount of salting-out agent entering the connecting ring 33. Conversely, when the external threaded rod 3 moves outward, the lower end of the piston rod 8 will move from the higher part to the lower part of the T-groove 37 to increase the amount of salting-out agent entering the connecting ring 33. After adjustment, tighten the two internal threaded rings 39 again so that the external threaded rod 3 cannot rotate.

[0053] The connecting motor 34 is turned on, driving the connecting gear 40 to rotate. The connecting gear 40 drives the connecting gear ring 19 to rotate, which in turn drives the rotating ring 11 to rotate counterclockwise. When the connecting cavity 32 moves to the upper end, under the vibration of the two vibrators 15, the salting agent in the storage hopper 4 will enter the connecting ring 33 for storage. As the rotating ring 11 continues to rotate, the connecting ring 33 containing the salting agent will rotate to the position of the arc-shaped heating plate 47. The arc-shaped heating plate 47 then heats the salt inside the connecting ring 33. The reagent is heated and dried, and during the rotation of the rotating ring 11, the curved rod 35 will come into contact with the arc-shaped guide plate 9. As the rotating ring 11 continues to rotate, the curved rod 35 will be guided upward by the arc-shaped guide plate 9. At this time, the gas in the gas storage chamber 44 will be pushed into the connecting chamber 32. The gas entering the connecting chamber 32 will enter the through chamber 29, so that one end of the T-shaped tube 30 is pushed into the inner side of the connecting ring 33. When the T-shaped tube 30 moves to the maximum stroke, the gas will continue to push the T-shaped rod 31 to move.

[0054] When the piston rod 8 moves upward to block several T-tubes 30, after some T-tubes 30 and T-rod 31 extend, the excess gas will push the push plate 42 to move, thereby allowing the gas to enter the relief cavity 41, ensuring that the curved rod 35 can always move along the arc guide plate 9.

[0055] During the rotation of the rotating ring 11, the toothed groove 38 on the outer side of the connecting ring 33 meshes with the first toothed ring 46. Therefore, when the rotating ring 11 moves the connecting ring 33, the connecting ring 33 will start to rotate. The rotation of the connecting ring 33 drives the rotation of several extended T-shaped tubes 30 and T-shaped rods 31, so that the salting-out agent stored in the connecting ring 33 is stirred and broken up, which makes it easier for the arc-shaped heating plate 47 to fully heat and dry the salting-out agent. When the connecting ring 33 containing the salting-out agent moves to the lower end, the salting-out agent will fall into the mixing aluminum cylinder 17 and mix with the crude enzyme solution. At this time, as the rotating ring 11 continues to rotate, the curved rod 35 will no longer be in contact with the arc-shaped guide plate 9. At this time, under the elastic force of the first spring 5 and the second spring 14, the T-shaped tubes 30 and T-shaped rods 31 retract to the initial position to prevent the T-shaped tubes 30 and T-shaped rods 31 from extending, so that the clumped salting-out agent cannot enter the connecting ring 33 for storage through the connecting cavity 32.

[0056] By turning on the drive motor 10, the drive gear 12 is driven to rotate. Since the drive gear 12 is located between the T-shaped toothed tube 13 and the T-shaped toothed column 28, the T-shaped toothed tube 13 and the T-shaped toothed column 28 will rotate in opposite directions during the rotation of the drive gear 12. The rotation of the T-shaped toothed tube 13 drives the turntable 16 to rotate, and the rotation of the turntable 16 drives the mixing aluminum cylinder 17 to rotate. Since the outer side of the limiting rod 25 is square and the limiting rod 25 is inserted into the square insertion hole 24, when the T-shaped toothed column 28 rotates, the connecting shaft 23 will drive the two stirring rods 7 to rotate. Through the mixing aluminum cylinder 17 and the stirring rods 7 rotating in opposite directions, the salting-out agent and the crude enzyme solution are fully mixed and reacted. After the reaction is completed and the mixture of glucose oxidase and other materials precipitates, the box door 6 can be opened to remove the mixing aluminum cylinder 17.

[0057] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A glucose oxidase preparation apparatus, comprising a salting-out tank, a conveying tank fixedly connected to the upper end of the salting-out tank, the salting-out tank and the conveying tank being in communication, and a storage hopper fixedly connected to the upper end of the conveying tank, the storage hopper being in communication with the conveying tank, characterized in that: A rotating ring is movably connected inside the conveying box. Connecting cavities are provided around the outer perimeter of the rotating ring. Connecting rings are movably connected inside the connecting cavities. One end of the connecting ring extends into the conveying box. Several toothed grooves are provided on the outer side of the connecting ring. Several telescopic stirring mechanisms are provided inside the connecting ring. The rotation of the connecting ring drives the extended telescopic stirring mechanisms to rotate, thereby stirring the salting-out agent. The toothed grooves mesh with a first toothed ring, which is fixedly connected to the inside of the conveying box. The connecting ring is provided with a clearance mechanism on both sides, which is located inside the rotating ring. The clearance mechanism allows the gas entering the connecting cavity to make way. The lower end of the connecting cavity is connected to two gas storage chambers. The gas storage chambers are located inside the rotating ring. A curved rod is slidably connected inside the gas storage chamber. The end of the curved rod away from the gas storage chamber extends into the material conveying box. An arc-shaped guide plate is provided inside the material conveying box. A piston rod is movably connected inside the connecting cavity. The lower end of the piston rod extends into the material conveying box and is connected to an adjustment mechanism. The adjustment mechanism is located inside the material conveying box. The height of the piston rod in the connecting cavity is adjusted by the adjustment mechanism. A limit spring is fixedly connected inside the gas storage chamber. The other end of the limit spring is fixedly connected to the curved rod. A turntable is movably connected to the bottom of the inner side of the salting-out tank. The upper end of the turntable is clamped by two clamping mechanisms to hold a mixing aluminum cylinder. The lower end of the mixing aluminum cylinder is arc-shaped. The clamping mechanism is located on the upper end of the turntable. A stirring mechanism is provided inside the mixing aluminum cylinder. The lower end of the stirring mechanism is connected to a transmission mechanism. The transmission mechanism is located inside the salting-out tank. An arc-shaped heating plate is fixedly connected to the inner side of the conveying box.

2. The glucose oxidase preparation apparatus according to claim 1, characterized in that: The telescopic stirring mechanism includes a T-shaped tube, which is slidably connected to the through cavity. The through cavity is opened in the connecting ring. A first spring is sleeved on the outside of the T-shaped tube. One end of the first spring is fixedly connected to the T-shaped tube, and the other end is fixedly connected to the through cavity.

3. The glucose oxidase preparation apparatus according to claim 2, characterized in that: A T-shaped rod is movably connected inside the T-shaped tube. A second spring is sleeved on the outside of the T-shaped rod. One end of the second spring is fixedly connected to the T-shaped rod, and the other end is fixedly connected to the inside of the T-shaped tube.

4. The glucose oxidase preparation apparatus according to claim 1, characterized in that: The yielding mechanism includes a push plate that slides within a yielding cavity. The yielding cavity is located within a rotating ring and is connected to a connecting cavity. Two third springs are fixedly connected to one side of the push plate, and the end of each third spring away from the push plate is fixedly connected to the yielding cavity.

5. The glucose oxidase preparation apparatus according to claim 1, characterized in that: The adjusting mechanism includes an external threaded rod, which is screwed into the material conveying box, with both ends of the external threaded rod extending into the external environment. A conical ring is movably connected to the outside of the external threaded rod, and T-slots are provided around the outside of the conical ring. The lower end of the piston rod slides into the T-slots, and two internal threaded rings are screwed to the outside of the external threaded rod.

6. The glucose oxidase preparation apparatus according to claim 1, characterized in that: The clamping mechanism includes a clamping rod, the lower end of which is slidably connected to a T-shaped guide groove. The T-shaped guide groove is located on the upper end of the turntable. A support spring is fixedly connected inside the T-shaped guide groove. The other end of the support spring is fixedly connected to the clamping rod. One end of the clamping rod is engaged in a snap-fit ​​hole located on the outside of the mixed aluminum cylinder.

7. The glucose oxidase preparation apparatus according to claim 1, characterized in that: The stirring mechanism includes a connecting shaft, which is movably connected to the lower end of the mixing aluminum cylinder. The upper end of the connecting shaft extends into the inner side of the mixing aluminum cylinder and is fixedly connected to two stirring rods. A square insertion hole is provided at the lower end of the connecting shaft, and the lower end of the square insertion hole is arc-shaped.

8. The glucose oxidase preparation apparatus according to claim 1, characterized in that: The transmission mechanism includes a T-shaped toothed column, which is movably connected inside a T-shaped toothed tube. The T-shaped toothed tube is movably connected inside the salting-out tank. The upper end of the T-shaped toothed tube is fixedly connected to the lower end of the turntable. The upper end of the T-shaped toothed column passes through the turntable. A transmission gear meshes between the T-shaped toothed column and the T-shaped toothed tube. The transmission gear is fixedly connected to the output end of a transmission motor. The transmission motor is fixedly connected to the salting-out tank. A sliding cavity is provided at the upper end of the T-shaped toothed column. A limit rod slides within the sliding cavity. The outer side of the limit rod is square. The upper end of the limit rod is inserted into a square socket. A return spring is fixedly connected within the sliding cavity. The upper end of the return spring is fixedly connected to the lower end of the limit rod.

9. The glucose oxidase preparation apparatus according to claim 1, characterized in that: Two vibrators are fixedly connected inside the storage hopper. A door is movably connected to one side of the salting-out box. A connecting gear ring is fixedly connected to one side of the rotating ring. The inner side of the connecting gear ring meshes with a connecting gear. A connecting motor is fixedly connected to the outside of the conveying box. The input end of the connecting motor extends into the conveying box and is fixedly connected to the connecting gear.

Images