An apparatus for enriching arbuscular mycorrhizal fungi and a method for enhancing inoculation effect of arbuscular mycorrhizal fungi in soil

Abstract

The application discloses an apparatus for enriching arbuscular mycorrhizal fungi, comprising an arbuscular mycorrhizal fungus enrichment and storage device (1) and a sealing cover (2); wherein the inner cavity of the arbuscular mycorrhizal fungus enrichment and storage device (1) is separated into a nutrient storage and mycelium chamber (101) and a pre-planting mycorrhizal chamber (102) by a partition (104). By sowing clover and inoculating arbuscular mycorrhizal fungi in the pre-planting mycorrhizal chamber for culture, the mycelium of the arbuscular mycorrhizal fungi will enter the nutrient storage and mycelium chamber and absorb soluble phosphorus nutrients, thereby strengthening the symbiosis with plants, and at the same time, the mycelium will recruit phosphorus-solubilizing bacteria to utilize insoluble calcium phosphate, thereby strengthening the enrichment of the arbuscular mycorrhizal fungi mycelium. When the apparatus containing active arbuscular mycorrhizal fungi is buried in the soil around the root system of a crop, a strong symbiotic niche can be provided for the arbuscular mycorrhizal fungi, thereby effectively enhancing the inoculation effect of the arbuscular mycorrhizal fungi in the soil.

Description

Technical Field

[0001] This application relates to the field of agricultural growth technology, and in particular to a device for enriching and storing arbuscular mycorrhizal fungi and a method for enhancing the inoculation effect of arbuscular mycorrhizal fungi in soil. Background Technology

[0002] Mycorrhizal fungi, as beneficial symbiotic fungi that live in mutual benefit with plants, can promote nutrient absorption and increase plant resistance to biotic and abiotic stresses, thus possessing enormous application potential in agricultural and forestry cultivation. Among them, arbuscular mycorrhizal fungi (AMF), capable of establishing symbiotic relationships with most terrestrial vascular plants (such as corn, wheat, soybeans, fruit trees, forest trees, and herbaceous plants), have a wide range of applications and great potential. Currently, there are more than 300 species of AMF, belonging to 11 families and 27 genera. Commonly used AMFs include *Rhizocystis*, *Rhizocystis*, *Rhizocystis*, *Rhizocystis*, and *Rhizocystis spp.*

[0003] However, under actual conditions, due to the competition among various microorganisms in the soil, inoculating plants with AMF or planting already inoculated plants often fails to produce a significant mycorrhizal fungal inoculation effect. Furthermore, as the planting time increases and the plants bear fruit or are harvested, the ecological niche of AMF in the soil will significantly decrease or even disappear completely. In the next planting season, it is still necessary to inoculate with AMF or plant inoculated plants, but the inoculation effect depends entirely on the real-time competition between AMF and other microorganisms in the soil, and the competitive advantage of the ecological niche in the early stage is not obvious.

[0004] Therefore, there is currently a lack of a system and technology that can create a favorable ecological competitive advantage for AMF inoculation in advance and continuously provide and enhance the effect of AMF inoculation, thereby improving the AMF inoculation effect of host plants under field conditions. Summary of the Invention

[0005] The purpose of this invention is to overcome the above-mentioned shortcomings of the prior art and to provide a device for enriching and storing arbuscular mycorrhizal fungi and a method for enhancing the inoculation effect of arbuscular mycorrhizal fungi in soil.

[0006] The first objective of this invention is to provide an apparatus for enriching and storing arbuscular mycorrhizal fungi.

[0007] A second objective of this invention is to provide a product containing and enriched arbuscular mycorrhizal fungi.

[0008] A third objective of this invention is to provide a method for enhancing the inoculation effect of arbuscular mycorrhizal fungi in soil.

[0009] To achieve the above objectives, the present invention is implemented through the following solution: This invention claims protection for an apparatus for enriching and storing arbuscular mycorrhizal fungi, comprising: an arbuscular mycorrhizal fungi enrichment and storage device and a sealing cap; The inner cavity of the arbuscular mycorrhizal fungi enrichment and storage device is divided into a nutrient storage and mycelium chamber and a pre-planted mycorrhizal chamber by a partition. The nutrient storage and mycelium chamber is located at the bottom of the pre-planted mycorrhizal chamber. The partition is provided with a mycelium entry hole for mycelium in the pre-planted mycorrhizal chamber to enter the nutrient storage and mycelium chamber. The nutrient storage and mycelium chamber is provided with a substrate isolation net that can be attached to the bottom of the mycelium entry hole. The pre-planted mycorrhizal chamber is provided with an external root isolation net that can be attached to the top of the mycelium entry hole. The side wall of the pre-planted mycorrhizal chamber is provided with a mycorrhizal chamber drainage hole and a mycorrhizal chamber mycelium passage hole. The mycorrhizal chamber drainage hole is located at the bottom of the side wall of the pre-planted mycorrhizal chamber. The external root isolation net of the pre-planted mycorrhizal chamber is provided on the external side wall that can be attached to the end face of the mycorrhizal chamber mycelium passage hole. The sealing cover can be placed on the top surface of the pre-planted mycorrhizal chamber; the arbuscular mycorrhizal fungi enrichment and storage device also includes an evaporation drainage pipe, one end of which extends into the nutrient storage and mycelium chamber, and the other end passes through the sealing cover.

[0010] Preferably, the evaporation drain pipe is located at the center of the arbuscular mycorrhizal fungi enrichment and storage device.

[0011] The evaporation drainage pipe is located at the center of the arbuscular mycorrhizal fungi enrichment and storage device. It can raise the water level through the principle of seepage irrigation and drain the potential water accumulation at the bottom of the device through the surface evaporation design, thereby effectively maintaining the stability of the nutrient storage and mycelial chamber nutrient levels and mycelial growth environment.

[0012] More preferably, the evaporator drain pipe is provided with a detachable evaporator, and the evaporator is provided with evaporation holes; The side of the evaporation hole located inside the evaporator is covered with a substrate-based leak-proof mesh; the evaporator is sealed by a pipe cover.

[0013] More preferably, the aperture of the substrate leak-proof isolation mesh is 0.8 to 1.5 mm.

[0014] More preferably, the aperture of the substrate leak-proof isolation mesh is 1mm.

[0015] Preferably, the diameter of the hyphae entry hole in the hyphae chamber is 0.5-1 cm; the diameter of the hyphae passage hole in the mycorrhizal chamber is 0.5-1 cm; and the diameter of the drainage hole in the mycorrhizal chamber is 1-2 cm.

[0016] More preferably, the diameter of the hyphae entry hole in the hyphae chamber is 1 cm; the diameter of the hyphae passage hole in the mycorrhizal chamber is 1 cm; and the diameter of the drainage hole in the mycorrhizal chamber is 2 cm.

[0017] Preferably, the aperture of the outdoor mycelial root isolation net, the indoor mycelial substrate isolation net, and the outdoor mycorrhizal root isolation net is 20–40 µm.

[0018] More preferably, the pore size of the outdoor mycelial root isolation net, the indoor mycelial matrix isolation net, and the outdoor mycorrhizal root isolation net is 30µm.

[0019] More preferably, the outdoor root isolation net for mycelium, the indoor substrate isolation net for mycelium, and the outdoor root isolation net for mycorrhizae are stainless steel mesh with a pore size of 30µm.

[0020] The mycelium chamber has multiple mycelium entry holes, mycelium passage holes in the mycorrhizal chamber, and evaporation holes, which are arranged in an array on the partition, the side wall of the pre-planted mycorrhizal chamber, and the evaporator.

[0021] Preferably, the outer wall of the arbuscular mycorrhizal fungi enrichment and storage device is an inclined sidewall, and the distance between the top of the inclined sidewall and the axis of the evaporation drain pipe is greater than the distance between the bottom of the inclined sidewall and the axis of the evaporation drain pipe.

[0022] The outer wall of the arbuscular mycorrhizal fungi enrichment and storage device is an inclined sidewall, which makes it difficult for external water to enter the device through gravity or osmosis. Moreover, its inward inclination angle can utilize the Coanda Effect to allow potential water in the soil to flow along the mycorrhizal outdoor root isolation net attached to the outer wall to the bottom of the outer wall of the device, without entering the device.

[0023] Preferably, the partition is inclinedly arranged in the inner cavity of the arbuscular mycorrhizal fungi enrichment and storage device, and the distance between the top of the partition and the axis of the evaporation drain pipe is less than the distance between the bottom of the partition and the axis of the evaporation drain pipe.

[0024] The inclined partition within the arbuscular mycorrhizal fungi enrichment and storage device utilizes the Coanda effect to allow water potentially entering the nutrient storage and mycelial chambers to flow along the curved surface into the pre-planted mycorrhizal chamber and then drain through the mycorrhizal chamber drainage holes located at the bottom of the pre-planted mycorrhizal chamber. This helps maintain the nutrient conditions in the nutrient storage and mycelial chambers without being disturbed by the potentially entering water, thus helping to maintain the ecological niche of the arbuscular mycorrhizal fungi within the device and facilitating the device's sustainable multi-cycle use. Furthermore, the inclined partition effectively increases the contact area between the pre-planted mycorrhizal chamber and the nutrient storage and mycelial chambers, thereby effectively improving the efficiency of mycelium entering the nutrient storage and mycelial chambers from the pre-planted mycorrhizal chambers and the nutrient utilization rate within the nutrient storage and mycelial chambers.

[0025] Preferably, the arbuscular mycorrhizal fungi enrichment and storage device is in the shape of an inverted frustum and further includes a bottom cover. The bottom cover is in the shape of an inverted cone, and the top surface of the bottom cover is provided with a receiving hole. The bottom of the arbuscular mycorrhizal fungi enrichment and storage device can extend into the receiving hole. When the arbuscular mycorrhizal fungi enrichment and storage device extends into the receiving hole, the bottom surface of the hole is in contact with the bottom surface of the arbuscular mycorrhizal fungi enrichment and storage device, and the sidewall of the hole is in contact with the sidewall of the arbuscular mycorrhizal fungi enrichment and storage device.

[0026] The bottom is made of PVC plastic or hard rubber, which is bonded to the arbuscular mycorrhizal fungi enrichment and storage device with an adhesive (double-sided tape) to form a conical structure. This allows the device to be effectively fixed in the soil, and the bottom seal protects the bottom of the arbuscular mycorrhizal fungi enrichment and storage device from being damaged by sand and gravel in the soil when it is inserted into the soil.

[0027] Preferably, the sealing cover is attached to the top surface of the pre-planted mycorrhizal chamber via a detachable connection.

[0028] Preferably, the arbuscular mycorrhizal fungi enrichment and storage device is made of transparent PVC.

[0029] The present invention also claims protection for a product enriched and stored with arbuscular mycorrhizal fungi, wherein seeds of herbaceous vascular plants are sown in the pre-planted mycorrhizal chamber of any of the above-described devices, and arbuscular mycorrhizal fungi are inoculated, cultured for 45 to 60 days, and the above-ground parts of the herbaceous vascular plants are cut off to obtain a product enriched and stored with arbuscular mycorrhizal fungi; wherein the herbaceous vascular plants are vascular plants capable of symbiotic relationships with arbuscular mycorrhizal fungi.

[0030] Preferably, the herbaceous vascular plant is clover.

[0031] Preferably, the arbuscular mycorrhizal fungus is *Heteromorpha rhizocarpium* (…). Rhizophagus irregularis ).

[0032] The present invention also claims protection for a method for enhancing the inoculation effect of arbuscular mycorrhizal fungi in soil, which involves burying the above-mentioned product in the soil around the roots of planted crops.

[0033] Meanwhile, the present invention also provides a method for enhancing the inoculation effect of arbuscular mycorrhizal fungi in soil using any of the above-described devices, comprising the following steps: S1. Seeds of herbaceous vascular plants are sown in the pre-planted mycorrhizal chamber of any of the above-described devices, and arbuscular mycorrhizal fungi are inoculated. After culturing for 45 to 60 days, the above-ground parts of the herbaceous vascular plants are cut off to obtain a device inoculated with arbuscular mycorrhizal fungi; wherein the herbaceous vascular plants are vascular plants that can coexist with arbuscular mycorrhizal fungi. S2. The device inoculated with arbuscular mycorrhizal fungi obtained in step S1 is buried in the soil around the roots of the planted crops.

[0034] Preferably, before sowing the seeds of vascular plants in the pre-planted mycorrhizal chamber in step S1, it is necessary to first fill the substrate of any of the above-mentioned devices, specifically including the following steps: S11. Separate the sealing cover from the arbuscular mycorrhizal fungi enrichment and storage device in any of the above-described devices, then insert the evaporation drain pipe into the nutrient storage and mycelium chamber, and remove the evaporator from the evaporation drain pipe, so that a gap appears between the evaporation drain pipe and the nutrient storage and mycelium chamber. S12. Fill the evaporation drain pipe inserted into the nutrient storage and mycelium chamber with quartz sand with a particle size of 1.5 mm. Then, through the gap between the evaporation drain pipe and the nutrient storage and mycelium chamber, inject the mixed substrate 1 with a phosphorus content of 5-10 g / Kg into the nutrient storage and mycelium chamber. After the nutrient storage and mycelium chamber is filled, completely wet the mixed substrate 1 in the nutrient storage and mycelium chamber with a 3 mM sodium hydrogen phosphate solution. The mixed matrix 1 is composed of quartz sand, vermiculite and calcium phosphate in a volume ratio of 65:65:1 to 2. S13. Reassemble the evaporator that was disassembled in step S11 with the evaporator drain pipe so that the gap that appeared in step S11 is sealed by the evaporator. Then fill the evaporator with quartz sand with a particle size of 1.5mm and cover the evaporator with a pipe cover. S14. When the liquid seeps out of the mycelium inlet hole of the nutrient storage and mycelium chamber without passing through the mycelium chamber and no liquid remains in the pre-planted mycorrhizal chamber, fill the pre-planted mycorrhizal chamber without the sealed cover with mixed substrate 2 to obtain the device filled with substrate. The mixed substrate 2 injected into the pre-planted mycorrhizal chamber is obtained by mixing 4mm clay particles and 2-3mm vermiculite in a volume ratio of 1:2.

[0035] Preferably, the arbuscular mycorrhizal fungus in step S1 is *Heterobryorrhizospora* (…). Rhizophagus irregularis ).

[0036] Preferably, the herbaceous vascular plant mentioned in step S1 is clover.

[0037] Preferably, the cultivation in step S1 specifically involves: during the cultivation process, 20 mL of Hoagland nutrient solution is poured into the pre-planted mycorrhizal chamber weekly, and daily watering is maintained; The phosphate concentration in the Hoagland nutrient solution is 100 μm.

[0038] Preferably, after cutting off the above-ground parts of the herbaceous vascular plants in step S1, the evaporator and the evaporation drain pipe are disassembled and separated, a sealing cover is placed on it, and then the evaporator is reassembled with the evaporation drain pipe. Then, 1.5 mm diameter quartz sand is used to fill the evaporator to obtain an apparatus inoculated with arbuscular mycorrhizal fungi.

[0039] Preferably, in step S2, when burying the device inoculated with arbuscular mycorrhizal fungi, the sealed cover of the device is kept exposed above the soil surface.

[0040] Compared with the prior art, the present invention has the following beneficial effects: This invention provides an apparatus for enriching and storing arbuscular mycorrhizal fungi, comprising an arbuscular mycorrhizal fungi enrichment and storage device (1) and a sealing cap (2); wherein the inner cavity of the arbuscular mycorrhizal fungi enrichment and storage device (1) is divided into a nutrient storage and mycelium chamber (101) and a pre-planted mycorrhizal chamber (102) by a partition (104). After sowing herbaceous vascular plants in the pre-planted mycorrhizal chamber and inoculating them with arbuscular mycorrhizal fungi for cultivation, the pre-planted mycorrhizal chamber contains a large number of active arbuscular mycorrhizal fungi symbionts, while the nutrient storage and mycelium chamber is rich in nutrients and only allows mycelia to pass through. The mycelia of the arbuscular mycorrhizal fungi will enter the chamber and absorb soluble phosphorus nutrients, thereby strengthening the symbiosis with the herbaceous vascular plants. At the same time, it will recruit phosphate-solubilizing bacteria to utilize insoluble calcium phosphate, further enhancing the large-scale enrichment of arbuscular mycorrhizal fungi mycelia. When this device, containing active arbuscular mycorrhizal fungi inoculum and symbionts, is buried in the soil around the roots of crops, it can provide a strong symbiotic ecological niche for arbuscular mycorrhizal fungi in the soil, thereby effectively enhancing the inoculation effect of arbuscular mycorrhizal fungi. It can also create favorable ecological niche competition conditions for arbuscular mycorrhizal fungi inoculation in all four rounds of no-till direct seeding.

[0041] Furthermore, the construction of the device for enriching and storing arbuscular mycorrhizal fungi not only utilizes the strategy of arbuscular mycorrhizal fungi specifically absorbing nutrients to increase the symbiosis of arbuscular mycorrhizal fungi within the device, but also utilizes the ecological niche of the strong arbuscular mycorrhizal fungi mycelium within the device to maintain the symbiotic advantage of arbuscular mycorrhizal fungi in the soil. This allows the entire device to maintain efficient mycorrhizal symbiosis of the target plants under field conditions, thereby enabling the target plants to achieve efficient mycorrhizal symbiosis when buried in this device during multiple planting cycles. In addition, the evaporation drainage pipe (103) design utilizes the principle of seepage irrigation and surface evaporation design to discharge the potential residual water at the bottom of the nutrient storage and mycelium chamber (101); at the same time, the arc surface design of the upper part of the nutrient storage and mycelium chamber (101) utilizes the Coanda effect to allow water that may enter the pre-planted mycorrhizal chamber (102) to enter the bottom of the pre-planted mycorrhizal chamber (102) along the arc surface and then be discharged. These two designs are conducive to maintaining the high nutrient conditions of the nutrient storage and mycelium chamber (101) without being disturbed by the potential water, thereby helping to maintain the ecological niche of arbuscular mycorrhizal fungi in the device, and thus facilitating the sustainable multi-round use of the device. Attached Figure Description

[0042] Figure 1 This is a schematic diagram of the structure of a device for enriching and storing arbuscular mycorrhizal fungi, as shown in Embodiment 1 of the present invention. Figure 2This is a diagram showing the usage state of a device for enriching and storing arbuscular mycorrhizal fungi according to Embodiment 2 of the present invention; 1-Arbuscular mycorrhizal fungi enrichment and storage device; 2-Sealed cover; 3-Protective bottom seal; 101-Nutrient storage and mycelium chamber; 102-Pre-planted mycorrhizal chamber; 103-Evaporation drainage pipe; 104-Partition section; 10101-Outdoor root isolation net for mycelium; 10102-Mycelium entry hole; 10103-Indoor substrate isolation net for mycelium; 10201-Outdoor root isolation net for mycorrhizal fungi; 10202-Mycorrhizal chamber mycelium penetration hole; 10203-Drainage hole for mycorrhizal chamber; 10301-Evaporator; 10302-Evaporation hole; 10303-Substrate leak-proof isolation net; 10304-Pipe cover. Detailed Implementation

[0043] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods; the materials and reagents used, unless otherwise specified, are commercially available.

[0044] Example 1: A device for enriching and storing arbuscular mycorrhizal fungi A device for enriching and storing arbuscular mycorrhizal fungi, such as Figure 1 As shown, it specifically includes: an arbuscular mycorrhizal fungus enrichment and storage device 1, a sealing cap 2, and a bottom seal 3; The arbuscular mycorrhizal fungi enrichment and storage device 1 is shaped like an inverted frustum. The inner cavity of the arbuscular mycorrhizal fungi enrichment and storage device 1 is divided into a nutrient storage and mycelium chamber 101 and a pre-planted mycorrhizal chamber 102 by a partition 104. The outer wall of the arbuscular mycorrhizal fungi enrichment and storage device 1 is an inclined sidewall, and the distance between the top of the inclined sidewall and the axis of the evaporation drain pipe 103 is greater than the distance between the bottom of the inclined sidewall and the axis of the evaporation drain pipe 103. The partition 104 is inclinedly arranged in the inner cavity of the arbuscular mycorrhizal fungi enrichment and storage device 1, and the distance between the top of the partition 104 and the axis of the evaporation drain pipe 103 is less than the distance between the bottom of the partition 104 and the axis of the evaporation drain pipe 103. The nutrient storage and mycelium chamber 101 is located at the bottom of the pre-planted mycorrhizal chamber 102, and the partition 104 is provided with mycelium entry holes 10102 for mycelium in the pre-planted mycorrhizal chamber 102 to enter the nutrient storage and mycelium chamber 101. The nutrient storage and mycelium chamber 101 is equipped with a mycelium chamber substrate isolation net 10103 that can be attached to the bottom of the mycelium entry hole 10102, and the pre-planted mycorrhizal chamber 102 is equipped with a mycelium chamber root isolation net 10101 that can be attached to the top of the mycelium entry hole 10102. The side wall of the pre-planted mycorrhizal chamber 102 is provided with a mycorrhizal chamber drainage hole 10203 and a mycorrhizal chamber hyphae passage hole 10202. The mycorrhizal chamber drainage hole 10203 is located at the bottom of the side wall of the pre-planted mycorrhizal chamber 102. The outer side wall of the pre-planted mycorrhizal chamber is provided with a mycorrhizal chamber outer root isolation net 10201 that can fit with the end face of the mycorrhizal chamber hyphae passage hole 10202. The mycelium inlet hole 10102 of the mycelium chamber has a diameter of 1 cm; the mycelium passage hole 10202 of the mycorrhizal chamber has a diameter of 1 cm; the drainage hole 10203 of the mycorrhizal chamber has a diameter of 2 cm; multiple mycelium inlet holes 10102 of the mycelium chamber and mycelium passage holes 10202 of the mycorrhizal chamber are provided, and they are arranged in an array on the partition and the side wall of the pre-planted mycorrhizal chamber. The mycelium outdoor root isolation net 10101, the mycelium indoor substrate isolation net 10103, and the mycorrhizal outdoor root isolation net 10201 are all stainless steel mesh with a 30µm aperture. The sealing cover 2 can be placed on the top surface of the pre-planted mycorrhizal chamber 102; The arbuscular mycorrhizal fungi enrichment and storage device 1 also includes an evaporation drain pipe 103, one end of which extends into the nutrient storage and mycelium chamber 101, and the other end passes through the sealing cover 2; the evaporation drain pipe 103 is located at the center of the arbuscular mycorrhizal fungi enrichment and storage device 1; a detachable evaporator 10301 is provided on the evaporation drain pipe 103, and an evaporation hole 10302 is provided on the evaporator 10301; the side of the evaporation hole 10302 located inside the evaporator 10301 is covered with a substrate leak-proof isolation net 10303; the evaporator 10301 is sealed by a pipe cover 10304; The substrate leak-proof isolation net has a plastic mesh with a pore size of 1mm; when the evaporator 10301 is removed from the evaporation drain pipe 103, a gap will appear between the evaporation drain pipe 103 and the nutrient storage and mycelium chamber 101. The bottom cover 3 is in the shape of an inverted cone. The top surface of the bottom cover 3 is provided with a receiving hole. The bottom of the arbuscular mycorrhizal fungi enrichment and storage device 1 can extend into the receiving hole. When the arbuscular mycorrhizal fungi enrichment and storage device 1 extends into the receiving hole, the bottom surface of the receiving hole is in contact with the bottom surface of the arbuscular mycorrhizal fungi enrichment and storage device (1), and the side wall of the hole is in contact with the side wall of the arbuscular mycorrhizal fungi enrichment and storage device (1). The nutrient storage and mycelium chamber 101 and the pre-planted mycorrhizal chamber 102 in the arbuscular mycorrhizal fungi enrichment and storage device 1 are integrally cast using a molded plastic casting process. The mycelium entry hole 10102, the mycelium passage hole 10202, and the drainage hole 10203 of the mycorrhizal chamber are formed during the casting process. The root isolation net 10101 outside the mycelium chamber, the substrate isolation net 10103 inside the mycelium chamber, and the root isolation net 10201 outside the mycorrhizal chamber are fixed in their respective positions after casting. The nutrient storage and mycelium chamber 101 and the pre-planted mycorrhizal chamber 102 are made of transparent PVC. The evaporation drainage pipe 103 in the arbuscular mycorrhizal fungi enrichment and storage device 1 is a hollow plastic pipe cast in a mold. The evaporator detachably connected to it is a plastic sleeve with evaporation holes 10302 cast in a mold. The substrate leak-proof isolation net 10303 is fixed in its respective position after the evaporator 10301 is cast. The bottom seal 3 is made of PVC plastic.

[0045] Example 2: A product enriched and stored with arbuscular mycorrhizal fungi A method for using a product containing enriched and stored arbuscular mycorrhizal fungi, illustrated in the following diagram: Figure 2 As shown, it includes the following steps: S1. The device shown in Example 1 is filled to obtain a device filled with the matrix, as follows: S11. Separate the sealing cap 2 and sealing bottom 3 from the arbuscular mycorrhizal fungi enrichment and storage device 1 in the device shown in Example 1. Then insert the evaporation drain pipe 103 into the bottom of the nutrient storage and mycelium chamber 101 and extend it out of the nutrient storage and mycelium chamber 101. Then remove the evaporator 10301 from the evaporation drain pipe 103, so that a gap appears between the evaporation drain pipe 103 and the nutrient storage and mycelium chamber 1. S12. Fill the evaporation drain pipe 103 inserted into the nutrient storage and mycelium chamber 101 with quartz sand with a particle size of 1.5 mm. Then, through the gap between the evaporation drain pipe 103 and the nutrient storage and mycelium chamber 101, inject a mixed matrix 1 with a phosphorus content of 5 g / Kg (composed of quartz sand, vermiculite and calcium phosphate in a volume ratio of 65:65:1) into the nutrient storage and mycelium chamber 101. After the nutrient storage and mycelium chamber 101 is filled, inject a 3 mM sodium hydrogen phosphate solution into the nutrient storage and mycelium chamber 101 using a syringe until the mixed matrix 1 is completely wetted. S13. Reassemble the evaporator 10301 that was disassembled in step S11 with the evaporator drain pipe 103 so that the gap that appeared in step S11 is sealed by the evaporator 10301. Then fill the evaporator 10301 with quartz sand with a particle size of 1.5mm and cover the evaporator 10301 with the pipe cover 10304. S14. After the liquid seeps out of the mycelium inlet hole 10102 of the nutrient storage and mycelium chamber 101 without passing through the mycelium chamber and no liquid remains in the pre-planted mycorrhizal chamber 102, the pre-planted mycorrhizal chamber 102 is filled with mixed substrate 2, and the device filled with substrate is obtained. The mixed matrix 2 is obtained by mixing 4mm clay particles with 2-3mm vermiculite in a volume ratio of 1:2. S2. Place the device filled with substrate obtained in step S1 vertically, open the tube cover 10304, and sow white clover in the pre-planted mycorrhizal chamber 102. Trifolium repens Seeds were inoculated with *Heteromorpha rhizocarpium* ( ) Rhizophagus irregularis The clover was cultivated for 2 months by watering it with 20 mL of Hoagland nutrient solution (phosphate concentration of 100 μm) every week and maintaining daily watering. After cultivation, the above-ground parts of the white clover were cut off, the evaporator 10301 and the evaporation drain pipe 103 were disassembled and separated, the sealing cover 2 was put on, and the evaporator 10301 was reassembled with the evaporation drain pipe 103. The evaporator 10301 was filled with quartz sand with a particle size of 1.5 mm. At the same time, the arbuscular mycorrhizal fungus enrichment and storage device 1 was inserted into the receiving hole of the bottom 3 and tightly fitted to obtain a product enriched and stored with arbuscular mycorrhizal fungi.

[0046] Example 3: A method for enhancing the inoculation effect of arbuscular mycorrhizal fungi in soil I. Experimental Methods The experimental group consisted of: a method for enhancing the inoculation effect of arbuscular mycorrhizal fungi in soil, comprising the following steps: S1. Obtain a product enriched with arbuscular mycorrhizal fungi according to the method shown in Example 2; S2. Raise ridges and build ditches in the field, with the ridges being 30cm high and the side ditches being 10cm deep; Next, the corn that has been potted and inoculated with Anomalae arbuscular mycorrhizal fungi is planted on the field ridges, and the product enriched and stored in step S1 is buried in four directions at 10-20cm from the base of the corn stem, for a total of 4 products, with the sealed cap 2 of each product exposed above the soil surface, and the four products are spaced 5-20cm apart. S3. Then carry out routine corn plant culture: use tap water for daily sprinkler irrigation of corn, and spray Hoagland nutrient solution (phosphate concentration adjusted to 100μm, nitrogen concentration adjusted to 2mM) once a month for culture; S4. After 90 days of cultivation, harvest the corn and remove the above-ground parts of the corn stalks. Sow corn seeds near the original corn root system using a no-till direct seeding method and cultivate them as shown in step S3.

[0047] Control group 1: The only difference from the experimental group is that in step S2, the product enriched and stored with arbuscular mycorrhizal fungi obtained in step S1 was not buried at four locations 10-20 cm away from the base of the corn stalk.

[0048] Control group 2: The only difference from the experimental group is that the corn planted on the ridges in step S2 was not inoculated with *Arbuscular mycorrhizal fungi*, and the product enriched and stored in step S1 was not buried in four locations 10-20 cm away from the base of the corn stem.

[0049] After repeating step S4 four times for the experimental group, control group 1 and control group 2 (a total of 5 rounds of corn harvesting), the corn plant height and aboveground biomass at each of the 5 rounds of corn harvesting were recorded.

[0050] II. Experimental Results Table 1 shows the maize plant height and aboveground biomass at the first harvest of maize in the experimental group, control group 1, and control group 2. Table 2 shows the maize plant height and aboveground biomass at the second harvest of maize. Table 3 shows the maize plant height and aboveground biomass at the third harvest of maize. Table 4 shows the maize plant height and aboveground biomass at the fourth harvest of maize. Table 5 shows the maize plant height and aboveground biomass at the fifth harvest of maize.

[0051] Table 1. Maize plant height and aboveground biomass at the first harvest of maize.

[0052] Table 2. Maize plant height and aboveground biomass at the second maize harvest.

[0053] Table 3. Maize plant height and aboveground biomass at the third round of maize harvest.

[0054] Table 4. Maize plant height and aboveground biomass at the fourth round of maize harvest.

[0055] Table 5. Maize plant height and aboveground biomass at the fifth round of maize harvest.

[0056] (Note: The data in the table above represent the mean ± standard deviation. The same letter after each column indicates that the difference between groups is not significant.) P >0.05, different letters after each column of data indicate significant differences between groups. P <0.05) The results showed that during the first to fourth harvests, the corn plant height and aboveground biomass in the experimental group (cultivated according to steps S1 to S3) were significantly higher than those in control groups 1 and 2. This indicates that using the device inoculated with arbuscular mycorrhizal fungi as shown in Example 2 to enrich and store arbuscular mycorrhizal fungi can effectively enhance the inoculation effect of arbuscular mycorrhizal fungi in the soil and increase crop yield. However, during the fifth harvest, although the corn plant height and aboveground biomass in the experimental group were higher than those in control groups 1 and 2, there was no significant difference. This is because the arbuscular mycorrhizal fungi in the device shown in Example 2 and the nutrients in the device were depleted during multiple planting rounds, thus failing to achieve an effective enrichment effect.

[0057] Therefore, after repeating the planting three times (i.e., harvesting four times), the device inoculated with arbuscular mycorrhizal fungi needs to be reconstructed as shown in step S1.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. For those skilled in the art, other variations or modifications can be made based on the above description and ideas, and it is neither necessary nor possible to exhaustively describe all implementation methods here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A device for enriching and storing arbuscular mycorrhizal fungi, characterized in that, include: Arbuscular mycorrhizal fungi enrichment and storage device (1) and sealing cap (2); The inner cavity of the arbuscular mycorrhizal fungi enrichment and storage device (1) is divided into a nutrient storage and mycelium chamber (101) and a pre-planted mycorrhizal chamber (102) by a partition (104). The nutrient storage and mycelium chamber (101) is located at the bottom of the pre-planted mycorrhizal chamber (102). The partition (104) is provided with a mycelium entry hole (10102) for mycelium in the pre-planted mycorrhizal chamber (102) to enter the nutrient storage and mycelium chamber (101). The nutrient storage and mycelium chamber (101) is provided with a mycelium chamber substrate isolation net (10103) that can fit against the bottom of the mycelium entry hole (10102). The pre-planted mycorrhizal chamber (102) is provided with a substrate isolation net (10103) that can fit against the bottom of the mycelium entry hole (10102). An outdoor root isolation net (10101) for mycorrhizal hyphae is attached to the top of the hyphae entry hole (10102). The side wall of the pre-planted mycorrhizal chamber (102) is provided with a mycorrhizal chamber drainage hole (10203) and a mycorrhizal chamber hyphae passage hole (10202). The mycorrhizal chamber drainage hole (10203) is located at the bottom of the side wall of the pre-planted mycorrhizal chamber (102). The outdoor root isolation net (10201) for mycorrhizal hyphae is provided on the outer side wall of the pre-planted mycorrhizal chamber. The sealing cover (2) can cover the top surface of the pre-planted mycorrhizal chamber (102); the arbuscular mycorrhizal fungi enrichment and storage device (1) also includes an evaporation drain pipe (103), one end of which extends into the nutrient storage and mycelium chamber (101), and the other end passes through the sealing cover (2).

2. The apparatus according to claim 1, characterized in that, The evaporation drain pipe (103) is located at the center of the arbuscular mycorrhizal fungi enrichment and storage device (1).

3. The apparatus according to claim 2, characterized in that, The evaporation drain pipe (103) is provided with a detachable evaporator (10301), and the evaporator (10301) is provided with evaporation holes (10302). The evaporation hole (10302) located inside the evaporator (10301) is covered with a matrix leak-proof isolation net (10303); the evaporator (10301) is sealed by a pipe cover (10304).

4. The apparatus according to claim 1, characterized in that, The outer side wall of the arbuscular mycorrhizal fungi enrichment and storage device (1) is an inclined side wall, and the distance between the top of the inclined side wall and the axis of the evaporation drain pipe (103) is greater than the distance between the bottom of the inclined side wall and the axis of the evaporation drain pipe (103).

5. The apparatus according to claim 1, characterized in that, The partition (104) is inclinedly arranged in the inner cavity of the arbuscular mycorrhizal fungi enrichment and storage device (1), and the distance between the top of the partition (104) and the axis of the evaporation drain pipe (103) is less than the distance between the bottom of the partition (104) and the axis of the evaporation drain pipe (103).

6. The apparatus according to claim 1, characterized in that, The arbuscular mycorrhizal fungi enrichment and storage device (1) is shaped like an inverted frustum; The device also includes a bottom cover (3), which is an inverted cone shape. The top surface of the bottom cover (3) is provided with a receiving hole. The bottom of the arbuscular mycorrhizal fungi enrichment and storage device (1) can extend into the receiving hole. When the arbuscular mycorrhizal fungi enrichment and storage device (1) extends into the receiving hole, the bottom surface of the receiving hole is in contact with the bottom surface of the arbuscular mycorrhizal fungi enrichment and storage device (1), and the side wall of the hole is in contact with the side wall of the arbuscular mycorrhizal fungi enrichment and storage device (1).

7. The apparatus according to claim 1, characterized in that, The sealing cover (2) is attached to the top surface of the pre-planted mycorrhizal chamber (102) via a detachable connection.

8. A product enriched and stored with arbuscular mycorrhizal fungi, characterized in that, Seeds of herbaceous vascular plants are sown in the pre-planted mycorrhizal chamber (102) of any of the devices described in claims 1 to 7, and arbuscular mycorrhizal fungi are inoculated. After culturing for 45 to 60 days, the above-ground parts of the herbaceous vascular plants are cut off to obtain a product enriched and stored with arbuscular mycorrhizal fungi; wherein the herbaceous vascular plants are vascular plants capable of symbiotic coexisting with arbuscular mycorrhizal fungi.

9. A method for enhancing the inoculation effect of arbuscular mycorrhizal fungi in soil, characterized in that, The product of claim 8 can be buried in the soil around the root system of the planted crop.

10. The method according to claim 9, characterized in that, When burying the product of claim 8 in the soil, keep the sealing cap (2) of the product exposed above the soil surface.

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