Phospholipid compositions comprising a surfactant and uses thereof

Abstract

The phospholipid composition containing a surfactant according to the present application contains a lysophospholipid and a sulfosuccinate-based surfactant, whereby the lysophospholipid as an effective ingredient can be well dispersed even in an aqueous solution, and the effective ingredient is not easily decomposed even if stored for a long time, and its efficacy can be maintained for a long time. Therefore, the phospholipid composition of the present application can inhibit the decomposition of the lysophospholipid as an effective ingredient, and thus the product can be stored for a long time, and thus, compared with the existing agricultural chemical composition having a short circulation period, it can be more easily used commercially.

Description

Technical Field

[0001] This invention relates to phospholipid compositions, and more specifically, to phospholipid compositions comprising surfactants. Background Technology

[0002] Lysophosphatidylethanolamine (LPE) is naturally found in the cells of animals and plants, particularly in egg yolks and brain cells. It is derived from phosphatidylethanolamine (PE), a type of phospholipid found in cell membranes. Phosphatidylethanolamine, abundant in egg yolks or soybean lecithin, is a phospholipid containing two fatty acids within its molecule. In vivo, phosphatidylethanolamine is converted to lysophosphatidylethanolamine (LPE) by removing one fatty acid through the action of phospholipase A2, a phospholipid hydrolase.

[0003] Lysophosphatidylethanolamine (LPE) is known to play a crucial role in fruit ripening and senescence. Treatment with LPE is known to inhibit senescence in tomato leaves and fruits, and it is also known to extend the shelf life of tomatoes when applied post-harvest. Furthermore, it is known that when applied to apples, it promotes anthocyanin formation in the peel and inhibits the loss of softening during storage. (Prior Patent Documents 1 and 2: U.S. Patent 5,110,341 and U.S. Patent 5,126,155) As mentioned above, lysophosphatidylethanolamine (LPE) is a very useful substance in the agricultural field. However, in its chemical structure, the ester bonds bound to fatty acids are highly sensitive to hydrolysis reactions based on the addition of water molecules, resulting in difficulties in maintaining stable formulations in aqueous solutions and long-term storage. Currently, in commercially available 10% aqueous solutions of lysophosphatidylethanolamine (LPE), the following phenomena typically occur: precipitation at temperatures below 20°C, or rapid decomposition of the active ingredient at temperatures above room temperature. Therefore, increasing the content of lysophosphatidylethanolamine (LPE), with its easily hydrolyzed chemical structure, as the active ingredient in products while maintaining a long shelf life is commercially important, but so far there is no effective solution to this problem. Summary of the Invention

[0004] The problem that the invention aims to solve In order to solve the problems described above, the present invention aims to provide a water-soluble phospholipid composition containing a surfactant.

[0005] In order to solve the problems described above, another problem that the present invention aims to solve is to provide a method for altering the health, growth, or life cycle of a plant or a portion thereof by treating it with a phospholipid composition.

[0006] In order to solve the problems described above, another problem that the present invention aims to solve is to provide various uses of a water-soluble phospholipid composition containing a surfactant.

[0007] On the other hand, the technical problems of the present invention are not limited to those mentioned above, and those skilled in the art can clearly understand other technical problems not mentioned through the following description.

[0008] means for solving problems To address the above-mentioned problems, one aspect of the present invention may include a phospholipid composition comprising a lysophospholipid represented by the following chemical formula 1 and a sulfosuccinate surfactant represented by the following chemical formula 2.

[0009] [Chemical Formula 1]

[0010] In the chemical formula 1, R1 is any one selected from saturated or unsaturated chain acyloxy groups of C5 to C24, saturated or unsaturated chain alkoxy groups of C5 to C24, and their derivatives. R2 is selected from hydrogen, hydroxyl, saturated or unsaturated chain acyloxy group from C1 to C5, and saturated or unsaturated chain alkoxy group from C1 to C5. R3 is selected from any one of hydrogen, choline, ethanolamine, glycerol, inositol, and serine. [Chemical Formula 2]

[0011] In the chemical formula 2, R4 and R5 are each independently hydrogen, sodium, or saturated or unsaturated chain or branched alkyl groups from C1 to C18, and R4 and R5 are not both hydrogen or sodium.

[0012] The derivative may have at least one hydroxyl group bonded to at least one carbon in the chemical structure of R1.

[0013] The phospholipid composition may be a liquid or powder form of a water-soluble composition.

[0014] The concentration of the lysophospholipid can be from 0.1 to 20% by weight relative to the total phospholipid composition.

[0015] The content of the sulfosuccinate surfactant may be from 0.1 to 20% by weight relative to the total weight of the phospholipid composition.

[0016] The phospholipid composition may further include fatty acid additives, which include: saturated or unsaturated chain fatty acids of C6 to C24; fatty acid metal salts containing any one of the metal salts selected from Na, K, Mg, Ca and Al; fatty acid organic salts containing any one of the organic salts selected from ethanolamine salts, ammonium salts, betaine, choline salts; or combinations of two or more of them.

[0017] Based on the total weight of the phospholipid composition, the content of the fatty acid additive can be from 1 to 20% by weight.

[0018] The phospholipid composition may further comprise an organic solvent, the organic solvent comprising: a monohydric alcohol, comprising at least one selected from ethanol, propanol, butanol, hexanol, octanol, and combinations thereof; a dihydric alcohol, comprising at least one selected from 1,3-butanediol, 2,3-butanediol, propylene glycol, hexanediol, octanediol, and combinations thereof; a trihydric alcohol, comprising glycerol; and mixtures of two or more thereof.

[0019] The phospholipid composition may also contain a combination of two or more of the following: lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and lecithin.

[0020] To address the other problems mentioned above, a method may be included, comprising the steps of: altering the health, growth, or life cycle of a plant or part of a plant by treating it with a plant treatment agent comprising a phospholipid composition.

[0021] The phospholipid composition may contain lysophospholipid represented by the following chemical formula 1 and sulfosuccinate surfactant represented by the following chemical formula 2.

[0022] [Chemical Formula 1]

[0023] In the chemical formula 1, R1 is any one selected from saturated or unsaturated chain acyloxy groups of C5 to C24, saturated or unsaturated chain alkoxy groups of C5 to C24, and their derivatives. R2 is selected from hydrogen, hydroxyl, saturated or unsaturated chain acyloxy group from C1 to C5, and saturated or unsaturated chain alkoxy group from C1 to C5. R3 is selected from any one of hydrogen, choline, ethanolamine, glycerol, inositol, and serine. [Chemical Formula 2]

[0024] In the chemical formula 2, R4 and R5 are each independently hydrogen, sodium, or saturated or unsaturated chain or branched alkyl groups from C1 to C18, and R4 and R5 are not both hydrogen or sodium.

[0025] The derivative may have at least one hydroxyl group bonded to at least one carbon in the chemical structure of R1.

[0026] The plant treatment agent may be an aqueous solution containing the lysophospholipid at a concentration of 1 to 5,000 ppm (w / w).

[0027] A portion of the plant may be selected from at least one of the following: fruit, leaf, flower, root, stem, and tuber.

[0028] The phospholipid composition may be a liquid or powder form of a water-soluble composition.

[0029] The plant treatment agent may also contain at least one selected from lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and combinations thereof.

[0030] The method may be a way to improve the quality of a plant or a part of a plant.

[0031] The quality may include at least one of the following: color, flavor, firmness, sugar content, fruit cracking, and aroma.

[0032] The method may be a method to delay the aging of a plant or a part of a plant.

[0033] Delaying aging can refer to extending the storage or preservation period of a plant or a part of a plant.

[0034] The method may be a method of increasing at least one of the diameter, width, weight, and water content of a plant, or a part thereof, selected from its fruit, vegetable, root, and / or tuber, by stimulating the growth of the plant or part thereof.

[0035] The method may be a means of protecting a plant or a part of a plant from biotic or abiotic stresses.

[0036] The abiotic stresses may include physical damage caused by at least one of the following abiotic causes: icing, wind, hail, flood, drought, extreme heat, and pesticides.

[0037] The pesticide may include at least one selected from plant growth regulators, insecticides, fungicides, herbicides, spreading agents, and fertilizers.

[0038] The biological stress may include physical damage caused by at least one biological cause, including pathogens, insects, nematodes, snails, mites, weeds, humans, and animals other than humans.

[0039] The pathogen may include at least one of fungi, protozoa, bacteria, and viruses.

[0040] The method may be a method for synthesizing components selected from plants or parts of plants, such as starch, protein, lipids, and combinations of two or more of them.

[0041] Invention Effects The phospholipid composition containing surfactants according to the present invention comprises lysophospholipids and sulfosuccinate-based surfactants, thereby ensuring that the lysophospholipids, as the active ingredient, are well dispersed even in aqueous solutions and are not easily decomposed even during long-term storage, thus maintaining efficacy for an extended period. Therefore, the phospholipid composition of the present invention can inhibit the decomposition of the lysophospholipids, as the active ingredient, thereby enabling long-term product storage and making it easier to use commercially compared to existing pesticide compositions with short shelf lives.

[0042] The effects of the present invention are not limited to those mentioned above, and those skilled in the art will clearly understand other effects not mentioned through the following description. Attached Figure Description

[0043] Figure 1 This is the result of the change in residual amount during storage of a type of water-soluble phospholipid composition of a dosage form with improved long-term storage stability according to an embodiment of the present invention.

[0044] Figure 2 This is the result of an experiment on the effect of a phospholipid composition according to an embodiment of the present invention on the prevention and treatment of kiwifruit canker.

[0045] Figure 3 The results are from an experiment on the inhibitory effect of a phospholipid composition according to an embodiment of the present invention on the occurrence of frost damage in Red Dew apples.

[0046] Figure 4 This indicates the result of the change in the peel color of the Red Dew apple variety before harvest using a phospholipid composition according to an embodiment of the present invention.

[0047] Figure 5 The results are from an experiment conducted using a phospholipid composition according to an embodiment of the present invention to promote coloring in Picnic apples.

[0048] Figure 6 The results are from an experiment conducted using a phospholipid composition according to an embodiment of the present invention to promote color development in the Campbell Early grape variety.

[0049] Figure 7 The results are from an experiment conducted on the color-promoting effect of a phospholipid composition prepared according to an embodiment of the present invention and left for seven days.

[0050] Figure 8 The results are obtained by measuring the change in peel color of Picnic apples after one year using a phospholipid composition prepared according to an embodiment of the present invention.

[0051] Figure 9 The results are from an experiment conducted on promoting color development in the Campbell's early grape variety using a phospholipid composition prepared according to an embodiment of the present invention and left for seven days.

[0052] Figure 10 The results are from observations of the changes in the skin color of the Campbell's early grape variety after one year of preparation using a phospholipid composition prepared according to an embodiment of the present invention. Detailed Implementation

[0053] This invention can be modified in many ways and can have many forms. Specific embodiments will be illustrated in the accompanying drawings and described in detail herein. However, it should be understood that these embodiments do not limit the invention to the specific disclosed form, but rather include all modifications, equivalents, and substitutions encompassed within the spirit and technical scope of this invention. In describing the various drawings, similar reference numerals are used for similar constituent elements.

[0054] Unless otherwise defined, all terms used herein (including technical and scientific terms) shall have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Terms identical to those predefined in commonly used dictionaries shall be interpreted as having the same meaning as in the relevant technical text, and shall not be interpreted in an idealized or overly formal sense unless expressly defined in this application.

[0055] Throughout the description of this application, when a part is described as "including" a certain element, it means, unless specifically stated to the contrary, that other elements may also be included, rather than excluding other elements.

[0056] The terms “about,” “substantially,” etc., used throughout this application are used to indicate the material tolerance in their numerical value or close to its numerical value, and are intended to prevent unscrupulous infringers from improperly using the disclosure of accurate or absolute numerical values ​​mentioned to aid in understanding the invention.

[0057] Throughout this application, when a substance (solute) is described as being "dispersed" or "dissolved" in a composition (solvent), it means that the solute does not precipitate in the solvent but dissolves in a transparent and stable state into a homogeneous state.

[0058] Phospholipid Composition Regarding water-soluble formulations containing lysophosphatidylethanolamine (LPE), registered as a pesticide in the United States, there is a problem that the decomposition rate of lysophosphatidylethanolamine (LPE) increases over time with increasing storage temperature, and the residual amount of lysophosphatidylethanolamine (LPE) decreases. In particular, at a temperature of 80°C, lysophosphatidylethanolamine (LPE) decomposes within almost one month. On the other hand, at room temperature, a certain degree of product stability can be maintained up to 120 days, but after six to eight months from the time of product production, there is usually a loss of more than 20% of lysophosphatidylethanolamine (LPE), making it difficult to maintain a shelf life of more than one year. In contrast, the preferred phospholipid composition according to the present invention contains lysophosphatidylethanolamine as an active ingredient and a sulfosuccinate-based surfactant, thereby improving dispersibility in water-soluble solvents and inhibiting the decomposition of the active ingredient, thus ensuring a shelf life of at least two years.

[0059] First, the phospholipid composition of the present invention may contain lysophospholipid represented by the following chemical formula 1.

[0060] [Chemical Formula 1]

[0061] In the chemical formula 1, R1 is any one selected from saturated or unsaturated chain acyloxy groups of C5 to C24, saturated or unsaturated chain alkoxy groups of C5 to C24, and derivatives thereof; R2 is any one selected from hydrogen, hydroxyl, saturated or unsaturated chain acyloxy groups of C1 to C5, and saturated or unsaturated chain alkoxy groups of C1 to C5; and R3 is any one selected from hydrogen, choline, ethanolamine, glycerol, inositol, and serine.

[0062] Specifically, R1 is any one of saturated or unsaturated chain acyloxy groups and their derivatives selected from C5 to C24, R2 is a hydroxyl group, and R3 is an ethanolamine.

[0063] More specifically, R1 is a C5 to C24 saturated or unsaturated chain acyloxy group, which can refer to a saturated fatty acid without a double bond or an unsaturated fatty acid whose chain has at least one double bond. R1 is a C5 to C24 saturated fatty acid; in other words, it is an acyloxy group with a saturated carbon chain. As an example, it can be selected from pentanoyloxy, hexanoyloxy, heptanoyloxy, octanoyloxy, nonanoyloxy, decanoyloxy, undecanoyloxy, dodecanoyloxy, tridecanoyloxy, tetradecanoyloxy, pentadecanoyloxy, etc. Any one of pentadecanoyloxy, hexadecanoyloxy, heptadecanoyloxy, octadecanoyloxy, nonadecanoyloxy, icosanoyloxy, heneicosanoyloxy, docosanoyloxy, tridecanoyloxy, tetracosanoyloxy, and their derivatives.Additionally, R1 is a C5 to C24 unsaturated fatty acid, which can be an acyloxy group having an unsaturated carbon chain containing a double bond. As an example, it can be selected from pentenoyloxy, hexenoyloxy, heptenoyloxy, octenoyloxy, nonenoyloxy, decenoyloxy, undecenoyloxy, dodecenoyloxy, tridecenoyloxy, and tetradecenoyloxy. any one of loxy, pentadecenoyloxy, hexadecenoyloxy, heptadecenoyloxy, octadecenoyloxy, nonadecenoyloxy, icosenoyloxy, heneicosenoyloxy, docenoyloxy, tricosenoyloxy, tetracosenoyloxy, and their derivatives. Additionally, R1 is a C5 to C24 unsaturated fatty acid, which can be an acyloxy group having an unsaturated carbon chain containing two double bonds. As an example, it can be any one selected from cis,cis-9,12-octadecadienoyloxy (derived from linoleic acid), cis,cis,cis-9,12,15-octadecatrienoyloxy (derived from linolenic acid), arachidonoyloxy (eicosatetraenoyloxy, derived from arachidonic acid), and their derivatives.

[0064] From the viewpoint of the very non-polarity of the plant surface, to allow the active ingredients to pass well through the plant surface or to allow the active ingredients to adhere well to the plant surface, R1 is preferably a C8 to 14 saturated fatty acid, more preferably a C8 to 12 saturated fatty acid, and most preferably a C10 to 12 saturated fatty acid, but is not limited thereto.

[0065] Furthermore, the derivative can be a derivative having at least one hydroxyl group bonded to at least one or more carbons in the chemical structure of R1. Specifically, the derivative can comprise a compound formed by reacting at least one hydroxyl group bonded to at least one unsaturated bond in R1, such as a position having a double bond, i.e., by a hydroxylation reaction, whereby the resulting lysophosphatidylethanolamine (hydroxylated LPE) can be used. Alternatively, it can refer to a compound having at least one hydroxyl group bonded or substituted in the chemical structure of R3. The hydroxylation reaction can be carried out, for example, by a chemical method utilizing hydrogen peroxide or similar peroxides, or by a biological method based on lipoxygenase, a lipid oxidase, etc.

[0066] More specifically, the chemical formula 1 can be represented by the following chemical formula 3.

[0067] [Chemical Formula 3]

[0068] In the chemical formula 3, R1' can be any of a saturated or unsaturated chain alkyl group selected from C5 to C24 and its derivatives. Furthermore, the derivative can be a derivative having at least one hydroxyl group bonded to at least one carbon atom in the chemical structure of R1'.

[0069] In one specific example, the chemical formula 3 may be lysophosphatidylethanolamine (LPE), but is not limited thereto.

[0070] The lysophosphatidylethanolamine (LPE) is a type of lysophospholipid, derived from phosphatidylethanolamine (PE). It can have the following chemical structure: phosphatidylethanolamine (PE) containing two fatty acids, a phosphate group, and an ethanolamine bound thereto in a glycerol backbone, with one of the two fatty acids removed from the sn-2 position.

[0071] The lysophosphatidylethanolamine (LPE) is a substance naturally found in plants and animals, and therefore, lysophosphatidylethanolamine (LPE) isolated and purified from natural substances can be used. In particular, it can be isolated from lecithin in soybeans, egg yolks, or rapeseed, etc. Crude soybean lecithin (commonly referred to as crude lecithin), generated as a byproduct in the soybean oil preparation process, consists of 60 to 70% polar lipids (phospholipids / glycolipids), 27 to 39% soybean oil, 1 to 3% water, and 0.5 to 3% other components. The polar lipids are purified by removing soybean oil, which is a neutral lipid contained in the crude lecithin, and can be soybean lecithin. Purified lecithin consists of 22 to 30% phosphatidylcholine (PC), 2 to 5% lysophosphatidylcholine (LPC), 16 to 22% phosphatidylethanolamine (PE), 0.5 to 2% lysophosphatidylethanolamine (LPE), 0.5 to 8% phosphatidic acid (PA), 0.1 to 3% phosphatidyl serine, 6 to 15% phosphatidylinositol, and others. On the other hand, in the case of egg yolk lecithin, it is also composed of 73 to 83% phosphatidylcholine (PC), 2 to 5% lysophosphatidylcholine (LPC), 13 to 17% phosphatidylethanolamine (PE), 1 to 3% lysophosphatidylethanolamine (LPE), and others. As mentioned above, since lecithin contains a very small amount of lysophosphatidylethanolamine (LPE), it is known that it is difficult to use commercially by directly isolating lysophosphatidylethanolamine (LPE) from these lecithins. Therefore, lysophosphatidylethanolamine (LPE) can be prepared by reacting lecithin with ethanolamine in the presence of phospholipase D and / or phospholipase A, which are phospholipid hydrolases.

[0072] Alternatively, lysophosphatidylethanolamine (LPE) can be obtained by hydrolyzing phosphatidylethanolamine (PE) extracted from natural substances into lysophosphatidylethanolamine (LPE), or by converting phosphatidylcholine (PC) into phosphatidylethanolamine (PE) and then hydrolyzing it into lysophosphatidylethanolamine (LPE).

[0073] Alternatively, hydrogenated lysophosphatidylethanolamine (LPE) can be used.

[0074] Furthermore, compounds with at least one hydroxyl group added to the unsaturated bond, such as a double bond, in the remaining fatty acid chain of the lysophosphatidylethanolamine (LPE) can also be used, namely hydroxylated lysophosphatidylethanolamine (hydroxylated LPE).

[0075] The concentration of the lysophospholipid relative to the total weight of the phospholipid composition can be from 0.1% to 20% by weight. Specifically, the concentration of the lysophospholipid can be from 0.2% to 10% by weight, more specifically, from 0.3% to 5% by weight, and in one specific example, from 0.5% to 5% by weight, but is not limited thereto. When lysophospholipids are contained at concentrations within the aforementioned range, they can be easily dispersed in aqueous solutions and can exhibit an effect of inhibiting the decomposition of the active ingredient in water-soluble solvents. On the other hand, when the concentration is less than the aforementioned range, the amount of the active ingredient in the composition is too small, which may make it difficult to exhibit the target efficacy. Conversely, when lysophospholipids are contained at concentrations exceeding the aforementioned range, the excessive amount of lysophospholipids contained in the water-soluble solvent may make it difficult to prepare a commercially viable homogeneous mixture.

[0076] Next, the phospholipid composition may contain a sulfosuccinate surfactant represented by the following chemical formula 2.

[0077] [Chemical Formula 2]

[0078] In the chemical formula 2, R4 and R5 are each independently hydrogen, sodium, or a saturated or unsaturated chain or branched alkyl group of C1 to C18, and R4 and R5 are not necessarily both hydrogen or sodium. Additionally, the chemical formula 2 may include a sulfosuccinate group as an anionic functional group and may exist in the form of an alkaline salt, such as a sodium salt.

[0079] Specifically, R4 and R5 can be C7 to C8 saturated or unsaturated chain or branched alkyl groups independent of each other. For example, the chemical formula 2 may include any one selected from dioctyl sulfosuccinate (docusate sodium), ammonium dinonyl sulfosuccinate, diamyl sodium sulfosuccinate, dicapryl sodium sulfosuccinate, diethylhexyl sodium sulfosuccinate, diheptyl sodium sulfosuccinate, dihexyl sodium sulfosuccinate, diisobutyl sodium sulfosuccinate, and ditridecyl sodium sulfosuccinate.

[0080] More specifically, in Formula 2, R4 and R5 can be hydrogen, sodium, or C8 saturated chain or branched alkyl groups independent of each other. However, R4 and R5 are not necessarily hydrogen or sodium. In one specific example, Formula 2 may include, but is not limited to, dioctyl sulfosuccinate (docusate sodium).

[0081] When the sulfosuccinate-based surfactant is included, it has the following effects: it improves the dispersion ability of the aforementioned lysophosphatidylcholine, such as lysophosphatidylethanolamine (LPE), which is an active ingredient of the present invention, in a water-soluble solvent, and on this basis, it inhibits the decomposition of lysophosphatidylethanolamine (LPE). Specifically, the fatty acid portion of the surfactant is hydrophobically bonded to LPE through physical bonds, such as van der Waals forces, while the hydrophilic portion of the sulfosuccinate functional group forms chemical bonds, such as ionic bonds, with lysophosphatidylethanolamine (LPE). Therefore, the existing problem of easy decomposition of lysophosphatidylethanolamine (LPE) in aqueous solution can be effectively suppressed. In addition, compared with surfactants containing other hydrophilic functional groups, such as carboxyl functional groups, the sulfosuccinate-based surfactant has more sites that can form chemical bonds with lysophosphatidylethanolamine (LPE), such as sites that can form ionic bonds, and thus has the ability to induce stronger differences in ionic bonds in terms of quantity and quality. Therefore, compared with the use of other types of surfactants, the use of sulfosuccinate surfactants can provide the advantage of enhanced LPE decomposition inhibition and maintain the efficacy of the active ingredient for a longer period of time.

[0082] The content of the sulfosuccinate surfactant can be from 0.1% to 20% by weight relative to the total weight of the phospholipid composition. Specifically, the content of the sulfosuccinate surfactant can be from 0.2% to 10% by weight, more specifically from 0.3% to 5% by weight, and in one specific example from 0.5% to 5% by weight, but is not limited thereto. When the sulfosuccinate surfactant is included within the above content range, it has the effect of improving the dispersibility of lysophosphatidylethanolamine (LPE) and inhibiting its decomposition in water-soluble solvents. On the other hand, when the content is less than the above range, the amount of sulfosuccinate surfactant in the composition is too small, and lysophosphatidylethanolamine (LPE) cannot be well dispersed in the aqueous solution, which may make it difficult to prepare a homogeneous phospholipid composition and may not exhibit the effect of inhibiting the decomposition of lysophosphatidylethanolamine (LPE). Conversely, when the content exceeds the range, the composition contains too much sulfosuccinate surfactant and relatively little lysophosphatidylethanolamine (LPE), the active ingredient, and therefore may not exhibit the efficacy of the target product.

[0083] In addition to the sulfosuccinate surfactants mentioned above as anionic surfactants, the phospholipid compositions of the present invention may also contain nonionic surfactants. The nonionic surfactant may be selected from polysorbate 20 (Tween). TM 20) Polysorbate 40 (Tween) TM40), Polysorbate 60 (Tween) TM 60), Polysorbate 65 (Tween) TM 65), Polysorbate 80 (Tween) TM 80), Polysorbate 85 (Tween) TM 85) Qulaton TM N-101, Qulaton TM X-100, Octylphenol Polyether 40, Nonoxynol Ether-9, Triethanolamine, Triethanolamine Peptide Oleate, Polyoxyethylene-660 Hydroxystearate (PEG-15, Solutol H15), Polyoxyethylene-35-Richard Oleate (Cremophor EL) TM At least one of the following: soybean lecithin, poloxamer, and combinations thereof. In one specific example, the nonionic surfactant may comprise polysorbate 80 (Tween). TM 80), but not limited thereto, may contain 0.1 to 5% by weight relative to the total weight of the composition, specifically 0.2 to 3% by weight, and more specifically 0.5 to 2% by weight. By adding a nonionic surfactant within the said content range, the dispersibility of lysophosphatidylethanolamine (LPE) in aqueous solution can be further improved without impairing the effect of sulfosuccinate surfactants.

[0084] Furthermore, the phospholipid composition of the present invention must contain a water-soluble solvent, such as water, and may further contain an organic solvent. The organic solvent may contain alcohols. Specifically, the organic solvent may contain: a monohydric alcohol, comprising at least one selected from ethanol, propanol, butanol, hexanol, octanol, and combinations thereof; a dihydric alcohol, comprising at least one selected from 1,3-butanediol, 2,3-butanediol, propylene glycol, hexanediol, octanediol, and combinations thereof; a trihydric alcohol, comprising glycerol; and mixtures of two or more thereof. In one specific example, the organic solvent may contain ethanol, glycerol, and / or mixtures thereof, but is not limited thereto.

[0085] Furthermore, the water-soluble phospholipid composition of the present invention may also contain fatty acid additives, said fatty acid additives comprising: saturated or unsaturated chain fatty acids of C6 to C24; fatty acid metal salts containing a metal salt selected from Na, K, Mg, Ca and Al; fatty acid organic salts containing an organic salt selected from ethanolamine salts, ammonium salts, betaine, choline salts; or combinations of two or more of them.

[0086] Based on the total weight of the phospholipid composition, the content of the fatty acid additive can be from 1 to 20% by weight, specifically from 5 to 17% by weight, and in one specific example from 10 to 15% by weight, but is not limited thereto. The presence of fatty acid additives within the aforementioned content range has the effect of increasing the solubility of lysophosphatidylethanolamine (LPE) and / or their derivatives in the water-soluble composition.

[0087] In addition, the phospholipid composition may also contain lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and combinations of two or more. In one example, the phospholipid composition may further contain lecithin.

[0088] In addition, the phospholipid composition may also contain metal masking agents such as EDTA 4Na (Ethylenediaminetetraacetic tetrasodium salt) and fertilizer nutrients such as dipotassium hydrogen phosphate (K2HPO4).

[0089] Furthermore, the phospholipid composition of the present invention can be a liquid or powder form of a water-soluble composition. Specifically, for ease of treatment of plants or parts thereof, such as by coating, spraying, etc., the phospholipid composition is preferably liquid. In one specific example, a liquid form using a water-soluble solvent is preferred, but it is not limited thereto, and can be processed or formulated in a form suitable for the intended use.

[0090] Plant treatment agents containing phospholipid compositions and their treatment methods The plant treatment agent of the present invention may contain a phospholipid composition. By treating a plant or a portion of a plant with a plant treatment agent containing the phospholipid composition, a variety of beneficial effects can be delivered. Specifically, a preferred plant treatment agent of the present invention may be a plant treatment agent diluted in a solvent to provide a concentration of the phospholipid composition suitable for treating the plant or a portion of a plant.

[0091] The phospholipid composition may include lysophospholipid represented by the following chemical formula 1 and sulfosuccinate surfactant represented by the following chemical formula 2.

[0092] [Chemical Formula 1]

[0093] In the chemical formula 1, R1 is any one selected from saturated or unsaturated chain acyloxy groups (C5 to C24), saturated or unsaturated chain alkoxy groups (C5 to C24), and their derivatives; R2 is any one selected from hydrogen, hydroxyl group, saturated or unsaturated chain acyloxy groups (C1 to C5), and saturated or unsaturated chain alkoxy groups (C1 to C5); and R3 is any one selected from hydrogen, choline, ethanolamine, glycerol, inositol, and serine. The specific contents of the chemical formula 1 are consistent with those described in the... Phospholipid Composition Some of the descriptions are the same.

[0094] Furthermore, the derivative can be a derivative having at least one hydroxyl group bonded to at least any one or more carbons in the chemical structure of R1. Specifically, the derivative can comprise a compound formed by a reaction in which at least one hydroxyl group is bonded to at least one unsaturated bond, such as a double bond, in R1, i.e., by a hydroxylation reaction, whereby the resulting lysophosphatidylethanolamine (hydroxylated LPE) can be used. Alternatively, it can refer to a compound having at least one hydroxyl group bonded to or substituted in the chemical structure of R3. The hydroxylation reaction can be carried out, for example, by a chemical method utilizing hydrogen peroxide or similar peroxides, or by a biological method based on lipoxygenase, a lipid oxidase, etc.

[0095] More specifically, the chemical formula 1 can be represented by the following chemical formula 3.

[0096] [Chemical Formula 3]

[0097] In the chemical formula 3, R1' can be any of a saturated or unsaturated chain alkyl group selected from C5 to C24 and its derivatives. Furthermore, the derivative can be a derivative having at least one hydroxyl group bonded to at least one carbon atom in the chemical structure of R1'.

[0098] In one specific example, the chemical formula 3 may be lysophosphatidylethanolamine (LPE), but is not limited thereto.

[0099] Next, the phospholipid composition may contain a sulfosuccinate surfactant represented by the following chemical formula 2.

[0100] [Chemical Formula 2]

[0101] In Formula 2, R4 and R5 are each independently hydrogen, sodium, or a saturated or unsaturated chain or branched alkyl group of C1 to C18. R4 and R5 are not necessarily both hydrogen or sodium. Additionally, Formula 2 may include a sulfosuccinate group as an anionic functional group and may exist in the form of an alkaline salt, such as a sodium salt. The specific details of Formula 2 are consistent with those described in [the original text]. Phospholipid Composition Some of the descriptions are the same.

[0102] More specifically, in Formula 2, R4 and R5 can be hydrogen, sodium, or C8 saturated chain or branched alkyl groups independent of each other. However, R4 and R5 are not necessarily hydrogen or sodium. In one specific example, Formula 2 may include, but is not limited to, dioctyl sulfosuccinate (docusate sodium).

[0103] According to a preferred embodiment of the present invention, the plant treatment agent can be prepared by diluting the phospholipid composition as an active ingredient by a factor of 10 to 10,000 in a solvent, such as water. The plant treatment agent may contain 1 to 5,000 ppm (w / w) of the lysophospholipid, specifically 1 to 3,000 ppm (w / w), more specifically 1 to 1,000 ppm (w / w), but is not limited thereto.

[0104] The part of the plant may be, for example, selected from at least one of the following: fruit, leaf, flower, root, stem, and tuber. The term "life cycle" may include two stages: before and after harvest of the plant or part of the plant.

[0105] The plant treatment agent of the present invention can exhibit effects such as improving characteristics and overall health status in plants or parts thereof, stimulating growth, and improving and delaying aging. The fruit may be selected from at least one of apples, pears, tomatoes, peaches, kiwis, plums, cherries, grapes, raspberries, blueberries, and strawberries, but is not limited thereto.

[0106] Based on these properties, the plant treatment agent of the present invention can be applied to various methods, thereby benefiting plants or agriculture. Specifically, the plant treatment agent of the present invention can improve the quality of plants or parts of plants, for example, at least one selected from color, flavor, firmness, sugar content, fruit cracking, and odor. In addition, the plant treatment agent of the present invention can exhibit the effect of delaying the aging of plants or parts of plants, thereby extending the storage period or shelf life of plants or parts of plants.

[0107] Furthermore, the plant treatment agent of the present invention can exhibit the effect of increasing the diameter, width, weight, and at least one of the following in water by stimulating the growth of plants or parts thereof: fruits, vegetables, roots and / or tubers selected from them.

[0108] Furthermore, the plant treatment agent of the present invention can exhibit the effect of protecting plants or parts of plants from biotic or abiotic stresses. Specifically, biotic stresses can include physical damage caused by at least one biological cause, including pathogens (e.g., fungi, protozoa, bacteria, viruses, etc.), insects, nematodes, snails, mites, weeds, humans, animals other than humans, and combinations thereof. Abiotic stresses can include physical damage caused by at least one nonbiological cause, including freezing, wind, hail, flood, drought, extreme heat, and pesticides (e.g., plant growth regulators, insecticides, fungicides, herbicides, spreading agents, fertilizers, etc.).

[0109] Furthermore, the plant treatment agent of the present invention can exhibit the effect of increasing the synthesis of components selected from plants or parts thereof, such as starch, protein, lipids, and combinations of two or more thereof.

[0110] Hereinafter, preferred experimental examples according to the present invention will be described in more detail with reference to the accompanying drawings for a more specific illustration. However, the present invention is not limited to the embodiments described herein, and may be embodied in other forms.

[0111] Examples 1 to 3: Preparation of phospholipid compositions with improved long-term storage stability A phospholipid composition was prepared by mixing the lysophospholipid (LPE) represented by Chemical Formula 1, the sulfosuccinate surfactant represented by Chemical Formula 2, the organic solvent (ethanol), water, the nonionic surfactant, glycerol, fatty acids, lecithin, and K2HPO4 at room temperature in the composition ratios specified in Table 1 below.

[0112] Comparative example: Phospholipid compositions containing conventional lysophospholipids The phospholipid compositions were prepared using the same method as in Examples 1 to 3, except that the sulfosuccinate surfactant represented by the chemical formula 2 was not used.

[0113] The composition and content of the phospholipid compositions corresponding to Examples 1 to 3 and the comparative examples of the present invention are summarized in Table 1 below.

[0114] [Table 1]

[0115] In Table 1 above, surfactant S represents sulfosuccinate surfactant.

[0116] Experimental Example 1: Evaluation of the storage stability of phospholipid compositions Figure 1 This is the result of the change in residual amount during storage of a type of water-soluble phospholipid composition of a dosage form with improved long-term storage stability according to an embodiment of the present invention.

[0117] Reference Figure 1 The residual LPE ratio in the phospholipid compositions of Examples 2 and 3 of the present invention was measured to confirm whether the LPE, as an active ingredient, would decompose and remain even during long-term storage. To evaluate the storage stability of the formulation using the water-soluble phospholipid compositions used in the examples of the present invention, the compositions were stored for up to ten weeks at a constant temperature of 54°C under stringent test conditions of six months at room temperature. The results showed that the phospholipid compositions containing sulfosuccinate surfactants according to Examples 2 and 3 maintained an LPE residual rate of over 94% even after five weeks, indicating significantly superior storage stability compared to the comparative examples. Therefore, it can be confirmed that the aqueous phospholipid compositions of the present invention can improve long-term storage stability even in aqueous solutions.

[0118] Therefore, in the test examples described later, it is preferable to use a phospholipid composition with a composition equivalent to that of Example 2 as the test group.

[0119] Experiment Example 2: Control Effect Test on Strawberry Powdery Mildew (Sphaerotheca aphanis) Under greenhouse cultivation conditions, the Red Pearl strawberry variety was used as the experimental crop. To test its control effect against powdery mildew (Sphaerotheca aphanis), foliar treatments were applied three times at seven-day intervals at the initial stage of the disease. Control group 1 received no treatment; control group 2 received a 4000-fold dilution of fluopyram wettable powder (main component content 30%); and the experimental group received a 1000-fold dilution of the phospholipid composition of this invention (Example 2, main component content 1%). The experimental plots were configured using a triple-replicated randomized block design, with three treatments, three replicates, a total of nine plots, and a required area of ​​180 m². 2 The experiment was conducted under the following conditions. The investigation method involved surveying the disease rate of all fruits in the experimental area. The experimental results, taken on the seventh day after treatment of the final product, are recorded in Table 2 below. The results confirm that the phospholipid composition of the present invention has a control effect against strawberry powdery mildew.

[0120] [Table 2]

[0121] Experimental Example 3: Treatment of Kiwifruit Ulcer Disease (Pseudomonas syringae pv. Actinidiae biovar) Prevention and control efficacy test Under open-field cultivation conditions, Jesy Gold kiwifruit was used as the experimental crop. To test the control effect against bacterial canker (Pseudomonas syringae pv. Actinidiae biovar), the target disease, three foliar treatments were applied at seven-day intervals at the initial stage of disease. Control group 1 received no treatment; control group 2 received a 1500-fold dilution of oxytetracycline-streptomycin sulfate wettable powder (main component content 20.3 wt%); and the experimental group received a 500-fold dilution of the phospholipid composition of this invention (Example 2, main component content 1 wt%). The experimental plots and required number of plants were determined using a three-replicated completely randomized design, with three treatments, three replicates, nine plots in total, one plant per plot, and a required number of nine plants. The number of diseased leaves was counted on 200 leaves in each experimental plot ten days after the final treatment.

[0122] Figure 2 This is the result of an experiment on the effect of a phospholipid composition according to an embodiment of the present invention on the prevention and treatment of kiwifruit canker.

[0123] The test results are for the seventh day after the final product treatment and are recorded in Table 3 below. Figure 2 As a result, it can be confirmed that the phospholipid composition of the present invention has a preventive effect against kiwi fruit canker.

[0124] [Table 3]

[0125] Experiment Example 4: Verification Experiment on Wheat Harvest and Increased Protein Content Under open-field cultivation conditions, the Forefront wheat variety was used as the experimental crop to test the effects on wheat yield and protein content. The control group received no treatment, while the experimental group used a 1000-fold dilution of the phospholipid composition of this invention (Example 2, main component content 1% by weight). Treatment was administered five days after anthesis. The experimental plots and required plant numbers were determined using a three-replicated completely randomized design, with two treatments, one replication, three experimental plots, each plot measuring 1 hectare, and a required area of ​​6 hectares. The yield was assessed by measuring bushel quantity (tons / ha) and protein content by using a whole grain analizer (%). The results are shown in Tables 4 and 5 below. Compared to the control group, the experimental group showed an increase in wheat yield of approximately 6% to 11% and an increase in wheat protein content of approximately 4% to 9%. Therefore, it can be confirmed that the phospholipid composition of the present invention has the effect of increasing wheat yield and protein content.

[0126] [Table 4]

[0127] [Table 5]

[0128] Experiment Example 5: Apple Frost Damage Inhibition Test Frost damage to fruit refers to the blackening of the stigma and ovules when damaged before or after flowering. In severe cases, the fruit may fail to flower and die, or even if it does flower, fertilization may fail, and the fruit stalk may bend, resulting in deformed fruit and premature fruit drop. Under open-field cultivation conditions, the 'Honglu' variety of apple was used as the experimental crop to test its inhibitory effect on frost damage. The control group received no treatment, while the experimental group used a 1000-fold dilution of the phospholipid composition of this invention (Example 2, main component content 1% by weight). The treatment period and method involved two foliar treatments at five-day intervals. The experimental plot configuration and required number of plants were determined using a three-replicated completely randomized design, with two treatments, three replicates, six total plots, one plant per plot, and six required plants. The investigation methods included: investigating the number of open central flowers per bud for flowering rate, and investigating the number of fertilized central flowers for fertilization rate.

[0129] Figure 3 The results are from an experiment using a phospholipid composition according to an embodiment of the present invention to inhibit frost damage in Red Dew apples. Figure 4This indicates the result of the change in the peel color of the Red Dew apple variety before harvest using a phospholipid composition according to an embodiment of the present invention.

[0130] The test results are shown in Tables 6 and 7 below. Figure 3 and Figure 4 As shown, compared with the control group of the present invention, the experimental group exhibited an average flowering rate that was about 1.8 times higher and an average fertilization rate that was about 1.4 times higher. Therefore, it can be confirmed that the phospholipid composition of the present invention exhibits an inhibitory effect on the occurrence of frost damage in apples.

[0131] [Table 6]

[0132] [Table 7]

[0133] Experiment Example 6: Verification Experiment on Apple Color Enhancement Apples of the Picnic variety were used as the experimental crop, and a color-promoting verification experiment (once) was conducted at the National Horticultural Science Institute of the Rural Development Agency. The experiment used 0 mg·L⁻¹ apples. -1 (Untreated), 20 mg·L -1 (Example 2 (1)) and 40 mg·L -1 (Example 2 (2)) The treatment concentration was achieved by spraying the phospholipid composition of the present invention (labeled LPE) with the following treatment methods: a first treatment was performed six weeks before harvest, a second treatment was performed four weeks before harvest, and a third treatment was performed two weeks before harvest. For the determination of apple coloration, the Hunter "a" value, which indicates the degree of redness of the peel color, was measured using a Hunter colorimeter. The higher the a value, the deeper the red color and the more it promotes apple coloration.

[0134] Figure 5 The results are from an experiment conducted using a phospholipid composition according to an embodiment of the present invention to promote coloring in Picnic apples.

[0135] As shown in Table 8 below and Figure 5 As shown, it can be confirmed that the results of measuring the change in apple peel color for the Picnic variety show that, in the test group treated with the phospholipid composition of the present invention, the a values ​​measured in both the reddest part of the fruit (labeled as red part) and the least red part (labeled as non-red parts) are high, especially at 20 mg·L⁻¹. -1Under the conditions of (Example 2 (1)), the measured value of a was the highest. This confirms that treatment with the phospholipid composition of the present invention can promote apple coloring.

[0136] [Table 8]

[0137] Next, using the Fuji variety as the experimental crop and the Qingsong apple orchard as the experimental site, a color-promoting verification experiment (two times) was conducted. The color was promoted at a dose of 0 mg·L⁻¹. -1 (Untreated) and 20 mg·L -1 (Example 2 (1)) The treatment concentration was obtained by spraying the phospholipid composition of the present invention. The treatment method was to perform the first treatment five weeks before harvest and the second treatment three weeks before harvest.

[0138] As shown in Table 9 below, it can be confirmed that, in the test group (labeled LPE treatment area) treated with the phospholipid composition of the present invention, the a values ​​measured for the reddest parts (labeled red parts) and the least red parts (labeled non-red parts) of the fruit were higher than those measured for the untreated area (control group). Therefore, it can be confirmed that treatment with the phospholipid composition of the present invention can promote apple coloring.

[0139] [Table 9]

[0140] Experiment Example 7: Verification Experiment on Grape Color Promotion The early-maturing variety of grape, *Vitis labrus claL.*, was used as the experimental crop, and a color-promoting verification experiment was conducted at grape growers' farms in Shixing City. The experiment used 0 mg·L⁻¹ of [amount missing] to promote grape color development. -1 (Untreated), 20 mg·L -1 (Example 2 (1)) and 40 mg·L -1 (Example 2 (2)) The treatment concentration was achieved by spraying the phospholipid composition of the present invention (labeled LPE) with the following treatment method: the first treatment was performed four weeks before harvest, and the second treatment was performed two weeks before harvest. For the determination of grape coloration, the color change was observed by the naked eye and the Hunter L value, a value, and b value, which indicate the degree of redness of the skin color, were measured using a Hunter colorimeter. The higher the a value, the deeper the red color; the higher the b value, the deeper the blue color; and the lower the L value, the closer the color is to black.

[0141] Figure 6 The results are from an experiment conducted using a phospholipid composition according to an embodiment of the present invention to promote coloring in the Campbell's early grape variety.

[0142] As shown in Table 10 below and Figure 6 As shown, it can be confirmed that the results of measuring the color change of grape skins for the Campbell's early varietal variety show that, compared with the untreated area, the color of all fruits in the test group treated with the phospholipid composition of the present invention is more vivid to the naked eye. It can be confirmed that the L value is reduced the most, resulting in a darker color, and that the a and b values ​​are also reduced, leading to a decrease in red and blue hues. Therefore, it can be confirmed that treatment with the phospholipid composition of the present invention can promote grape coloring.

[0143] [Table 10]

[0144] Test Example 8: Apple Coloring Promotion Test Based on the Formulation of Phospholipid Composition The following verification and evaluation were conducted: whether the phospholipid composition of the present invention, with improved storage stability, could exhibit apple coloring efficacy similar to conventionally used formulations, even after seven days of preparation. Apples of the Picnic variety were used as the test crop, and the apple coloring promotion verification experiment was conducted at the National Horticultural Science and Technology Institute of the Rural Development Agency. The results were compared between the untreated phospholipid composition (untreated) and the treatment with 20 mg / L... -1 After the preparation of the comparative example phospholipid composition, in a dosage form (labeled as comparative example (after seven days)) and when using 20 mg·L⁻¹ -1 After preparing the phospholipid composition according to Example 2 of the present invention, a seven-day formulation (labeled Example 2 (after seven days)) was subjected to foliar spraying treatment. The treatment method consisted of a first treatment six weeks before harvest and a second treatment four weeks before harvest. For the determination of apple coloration, the Hunter "a" value, which indicates the degree of redness of the peel color, was measured using a Hunter colorimeter. A higher "a" value indicates a deeper red color and promotes apple coloring.

[0145] Figure 7 The results are from an experiment conducted on the color-promoting effect of a phospholipid composition prepared according to an embodiment of the present invention and left for seven days.

[0146] As shown in Table 11 below and Figure 7As shown, it can be confirmed that the results of measuring changes in apple peel color for the Picnic variety based on the phospholipid composition formulation show that, compared with the untreated variety, the a values ​​measured in both the reddest part of the fruit (marked as red part) and the least red part (marked as non-red part) are higher, which can promote apple coloring.

[0147] [Table 11]

[0148] Test Example 9: Time-dependent apple coloring promotion test of phospholipid composition It was determined whether the content of LPE, the active ingredient in the phospholipid composition according to the present invention, varied after preparation depending on the storage period.

[0149] Referring to Table 12 below, it can be confirmed that the results of measuring the change in LPE content in the product containing the phospholipid composition show that, compared to the comparative example, the content in the example remained higher after seven days and twelve months post-manufacturing.

[0150] [Table 12]

[0151] Figure 8 The results are obtained by measuring the change in peel color of Picnic apples after one year using a phospholipid composition prepared according to an embodiment of the present invention.

[0152] Additionally, please refer to Table 13 below and Figure 8 It was confirmed that, after one year of use of the prepared product, the results of measuring the change in apple peel color for the Picnic variety showed that, compared with the untreated and comparative examples, the a values ​​measured in both the reddest (marked as red parts) and the least red (marked as non-red parts) parts of the fruit were higher, which promoted apple coloring. It was also confirmed that almost no difference in coloring was observed between the untreated and comparative examples.

[0153] [Table 13]

[0154] Experimental Example 10: Grape Color Enhancement Test Based on Phospholipid Composition Formulation The following verification and evaluation were conducted: whether the phospholipid composition of the present invention, with improved storage stability, could exhibit grape coloring efficacy similar to conventionally used formulations, even after seven days of preparation. The Campbell Early vine (Vitis labrus claL.) variety was used as the test crop, and grape coloring promotion verification experiments were conducted at grape growers' farms in Shixing City. The results were compared between the untreated phospholipid composition (untreated) and the treatment with 20 mg / L... -1The dosage form (labeled as Comparative Example (after seven days)) after seven days of preparation of the phospholipid composition of the comparative example, and the use of 20 mg·L -1 After preparing the phospholipid composition of the present invention, a seven-day formulation (labeled as Example 2 (after seven days)) was applied to the stems and leaves via spraying. The treatment method consisted of a first treatment four weeks before harvest and a second treatment two weeks before harvest. The coloration of the grapes was confirmed by visual observation.

[0155] Figure 9 The results are from an experiment conducted on promoting color development in the Campbell's early grape variety using a phospholipid composition prepared according to an embodiment of the present invention and left for seven days.

[0156] Reference Figure 9 It can be confirmed that the results of visual observation of the changes in grape skin color of the Campbell's early varietal grapes according to the formulation of the phospholipid composition showed that there was almost no difference between the formulations of the comparative example and Example 2, but compared with the untreated area, the treatment areas of the comparative example and Example 2 promoted visible grape coloring.

[0157] In addition, referring to Table 14 below, the anthocyanin content of Campbell's Early Grapes was determined based on the formulation used seven days after the preparation of the phospholipid composition. The anthocyanin content in the peel was determined using the following values: 10 peel discs were collected from the equatorial surface of the berries using a corkborer (5 mm), immersed in a 0.1N HCl-100% EtOH (15:85, V / V) solution, and stored in the dark under cold for 24 hours. The absorbance was then measured at 535 nm using a spectrophotometer (UV-2501 PC, Shimadzu, Japan), and the total anthocyanin content was calculated according to the method of Fuleki & Francis (1968a, 1968b). Therefore, it can be confirmed that in the LPE formulation (Example 2) prepared seven days after preparation, grape coloring was promoted by LPE treatment regardless of the grape variety, and the anthocyanin content increased.

[0158] [Table 14]

[0159] Experimental Example 11: Grape Coloring Promotion Test Based on Time Elapsed of Phospholipid Composition It was determined whether the content of LPE, the active ingredient in the phospholipid composition according to the present invention, varied after preparation depending on the storage period.

[0160] Referring to Table 15 below, it can be confirmed that the results of measuring the change in the content of LPE remaining in the product according to the shelf life of the phospholipid composition show that, compared with the comparative example, the content remained higher in the case of the example after seven days and twelve months after preparation.

[0161] [Table 15]

[0162] Figure 10 The results are based on observations of changes in the skin color of Campbell's early grape variety after one year of preparation using a phospholipid composition prepared according to an embodiment of the present invention, depending on the shelf life of the composition.

[0163] Reference Figure 10 It can be confirmed that, for the Campbell Early variety using the phospholipid composition prepared one year after the experiment, the change in grape skin color observed by the naked eye showed that there was almost no difference in coloring between the untreated and comparative examples, but the grapes of Example 2 were darker and coloring was promoted compared with the untreated or comparative examples.

[0164] Furthermore, referring to Table 16 below, it can be confirmed that, compared with the untreated or comparative examples, the concentration of anthocyanins in the Campbell Early grape variety remained high in the case of the examples.

[0165] [Table 16]

[0166] On the other hand, the embodiments of the present invention disclosed in this specification and accompanying drawings are merely specific examples shown to aid understanding and do not limit the scope of the invention. Those skilled in the art will appreciate that other modifications based on the technical concept of the present invention can be implemented in addition to the embodiments disclosed herein.

Claims

1. A phospholipid composition, wherein, The phospholipid composition comprises a lysophospholipid represented by the following chemical formula 1 and a sulfosuccinate surfactant represented by the following chemical formula 2: [Chemical Formula 1] In the chemical formula 1, R1 is any one selected from saturated or unsaturated chain acyloxy groups of C5 to C24, saturated or unsaturated chain alkoxy groups of C5 to C24, and their derivatives. R2 is selected from hydrogen, hydroxyl, saturated or unsaturated chain acyloxy group from C1 to C5, and saturated or unsaturated chain alkoxy group from C1 to C5. R3 is selected from any one of hydrogen, choline, ethanolamine, glycerol, inositol, and serine. [Chemical Formula 2] In the chemical formula 2, R4 and R5 are each independently hydrogen, sodium, or saturated or unsaturated chain or branched alkyl groups from C1 to C18, and R4 and R5 are not both hydrogen or sodium.

2. The phospholipid composition according to claim 1, wherein, The derivative has at least one hydroxyl group bonded to at least one carbon atom in the chemical structure of R1.

3. The phospholipid composition according to claim 1, wherein, The phospholipid composition is a water-soluble composition in liquid or powder form.

4. The phospholipid composition according to claim 1, wherein, The concentration of the lysophospholipid is 0.1 to 20% by weight relative to the total weight of the phospholipid composition.

5. The phospholipid composition according to claim 1, wherein, The content of the sulfosuccinate surfactant is 0.1 to 20% by weight relative to the total weight of the phospholipid composition.

6. The phospholipid composition according to claim 1, wherein, The phospholipid composition also contains fatty acid additives. The fatty acid additive comprises: Saturated or unsaturated chain fatty acids from C6 to C24. Fatty acid metal salts containing any one of the metal salts selected from Na, K, Mg, Ca, and Al; Organic salts containing fatty acid salts selected from any one of the organic salts chosen from ethanolamine salts, ammonium salts, betaine, and choline salts; or Two or more of them.

7. The phospholipid composition according to claim 6, wherein, Based on the total weight of the phospholipid composition, the content of the fatty acid additive is 1 to 20% by weight.

8. The phospholipid composition according to claim 1, wherein, The phospholipid composition also contains an organic solvent. The organic solvent comprises: Monohydric alcohols include at least one selected from ethanol, propanol, butanol, hexanol, octanol, and combinations thereof; Diols include at least one selected from 1,3-butanediol, 2,3-butanediol, propylene glycol, hexanediol, octanediol, and combinations thereof; Triols, including glycerol; and A mixture of two or more of them.

9. The phospholipid composition according to claim 1, wherein, The phospholipid composition further comprises lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and combinations of two or more thereof.

10. A method comprising the step of treating a plant or a portion of a plant with a plant treatment agent comprising a phospholipid composition, thereby altering the health status, growth, or life cycle of said plant or portion of the plant, wherein, The phospholipid composition comprises lysophospholipid represented by the following chemical formula 1 and a sulfosuccinate surfactant represented by the following chemical formula 2: [Chemical Formula 1] In the chemical formula 1, R1 is any one selected from saturated or unsaturated chain acyloxy groups of C5 to C24, saturated or unsaturated chain alkoxy groups of C5 to C24, and their derivatives. R2 is selected from hydrogen, hydroxyl, saturated or unsaturated chain acyloxy group from C1 to C5, and saturated or unsaturated chain alkoxy group from C1 to C5. R3 is selected from any one of hydrogen, choline, ethanolamine, glycerol, inositol, and serine. [Chemical Formula 2] In the chemical formula 2, R4 and R5 are each independently hydrogen, sodium, or saturated or unsaturated chain or branched alkyl groups from C1 to C18, and R4 and R5 are not both hydrogen or sodium.

11. The method according to claim 10, wherein, The derivative has at least one hydroxyl group bonded to at least one carbon atom in the chemical structure of R1.

12. The method according to claim 10, wherein, The plant treatment agent is an aqueous solution containing the lysophospholipid at a concentration of 1 to 5,000 ppm (w / w).

13. The method according to claim 10, wherein, A portion of the plant is selected from at least one of the following: fruit, leaf, flower, root, stem, and tuber.

14. The method of claim 10, wherein, The plant treatment agent is a water-soluble composition in liquid or powder form.

15. The method according to claim 10, wherein, The plant treatment agent further comprises at least one selected from lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and combinations thereof.

16. The method of claim 10, wherein, The method improves the quality of plants or parts of plants.

17. The method according to claim 16, wherein, The quality includes at least one of the following: color, flavor, firmness, sugar content, fruit cracking, and aroma.

18. The method according to claim 10, wherein, The method delays the aging of plants or parts of plants.

19. The method according to claim 18, wherein, Delaying aging refers to extending the storage or preservation period of a plant or part of a plant.

20. The method of claim 10, wherein, The method increases at least one of the following in water: the diameter, width, weight, and water content of the plant, or a portion thereof, selected from its fruit, vegetable, root, and / or tuber, by stimulating the growth of the plant or part thereof.

21. The method according to claim 10, wherein, The method protects plants or parts of plants from biotic or abiotic stresses.

22. The method according to claim 21, wherein, The abiotic stresses include physical damage caused by at least one of the following abiotic causes: icing, wind, hail, flood, drought, extreme heat, and pesticides.

23. The method according to claim 22, wherein, The pesticides include at least one selected from plant growth regulators, insecticides, fungicides, herbicides, spreading agents, and fertilizers.

24. The method according to claim 21, wherein, The biological stress includes physical damage caused by at least one biological cause, including pathogens, insects, nematodes, snails, mites, weeds, humans, and animals other than humans.

25. The method according to claim 24, wherein, The pathogens include at least one of fungi, protozoa, bacteria, and viruses.

26. The method according to claim 10, wherein, The method increases the synthesis of components selected from plants or parts of plants, including starch, proteins, lipids, and combinations of two or more of them.

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