Novel method for detecting neuronal antibodies

By culturing neural progenitor cells in a neuropromoting medium and optimizing the detection method, and incubating hiPSC-derived neurons with patient biofluids, the problem of difficult AE diagnosis in existing technologies has been solved, and efficient detection of neuronal surface antibodies has been achieved.

CN122161922APending Publication Date: 2026-06-05BELLWETHER INSTITUTE FOR BIOMEDICAL RESEARCH +4
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Patent Information

Application Number
CN202480071340.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2023-10-10
Filing Date
2024-10-10
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

Existing neuronal antibody detection methods are unable to effectively screen for neuronal surface antibodies in patients with autoimmune encephalitis (AE), leading to diagnostic difficulties, especially due to insufficient detection of novel antigens.

Method used

By culturing neural progenitor cells (NPCs) in a neuropromoting medium for at least three weeks, optimizing culture conditions and cell surface antibody detection methods, and incubating hiPSC-derived neuronal cells with patient biofluids, the binding of human antibodies to cells was detected.

Benefits of technology

It improves the detection sensitivity and specificity of neuronal antibodies, enabling more accurate identification of neuronal surface antibodies related to acute exacerbations (AEs) and improving the diagnostic efficacy of AEs.

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Abstract

The present invention provides a method for obtaining human induced pluripotent stem cell (hiPSC)-derived neuronal cells, which can be used to diagnose nervous system disorders associated with the presence of neuronal antibodies. In particular, the present invention provides a method for obtaining neuronal cells derived from hiPSC wherein the method comprises culturing neural progenitor cells in a pro-neural medium under optimized conditions for at least three weeks. The invention also covers the use of neuronal cells obtained by the method of the invention as a diagnostic test in nervous system disorders associated with the presence of neuronal antibodies and kits comprising the same.
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