Method for constructing an animal model of complex inflammation in middle-aged and elderly type and related application thereof

By injecting D-galactose and using sodium dextran sulfate solution in an animal model, a complex inflammatory animal model of middle-aged and elderly people was constructed. This solved the problem that existing models could not simulate the intestinal-hepatic axis dysfunction in middle-aged and elderly people, and achieved a realistic simulation of the pathological characteristics of middle-aged and elderly people and an evaluation of intervention substances.

CN122162751APending Publication Date: 2026-06-09WUXI LICHENG MEDICAL NUTRITION CO LTD +1
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Patent Information

Application Number
CN202610428759.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-02
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing animal models of colitis are insufficient to simulate the complex pathological state of intestinal inflammation, liver oxidative stress, and gut-liver axis dysfunction in middle-aged and elderly individuals, and cannot truly reflect the chronic inflammatory characteristics of this population.

Method used

By subcutaneously injecting D-galactose into adult animals after adaptive feeding, combined with drinking of sodium dextran sulfate solution, intestinal inflammatory damage and liver oxidative stress were induced, thus constructing a middle-aged and elderly complex inflammatory animal model to simulate the intestinal-liver axis dysfunction under relevant physiological conditions in middle-aged and elderly individuals.

Benefits of technology

A stable and reproducible complex inflammation model for middle-aged and elderly individuals was successfully constructed, which can simultaneously reflect intestinal inflammation, intestinal barrier damage, and abnormal liver oxidative stress, realistically simulating the pathological characteristics of middle-aged and elderly populations, and is suitable for the evaluation and research of intervention substances.

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Abstract

This invention belongs to the field of vertebrate technology, specifically relating to a method for constructing a complex inflammatory animal model in middle-aged and elderly individuals and its related applications. The method includes: selecting adult animals for adaptive feeding, daily subcutaneous injection of D-galactose to establish a susceptibility to aging and oxidative stress, and administering sodium dextran sulfate (DSS) solution within a specific time window to induce intestinal inflammation, forming a complex inflammatory state through gut-hepatic axis interactions. The constructed animal model simultaneously exhibits intestinal inflammatory damage, abnormal hepatic oxidative stress, and aging-related characteristics, realistically simulating the pathological process of gut-hepatic axis dysfunction in middle-aged and elderly individuals. This model is suitable for screening and evaluating drugs, medical nutrition products, and functional foods that improve gut-hepatic axis dysfunction, providing a reliable technical platform for research on related disease mechanisms and the development of interventional substances.
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Claims

1. A method for constructing a middle-aged and elderly animal model of complex inflammation based on gut-liver axis dysfunction, characterized in that, The construction method includes the following steps: (1) Select adult animals as model animals; (2) Adaptive feeding for 1-2 weeks to prepare for modeling; (3) D-galactose was injected subcutaneously daily during the modeling period; (4) On the 8th to 10th day of modeling, the model animals were given free access to a 3% to 5% sodium dextran sulfate solution every day; (5) On the 11th day of modeling, replace the sodium dextran sulfate solution with sterile drinking water; (6) On the 12th to 13th day of modeling, replace the sterile drinking water with a 2% to 5% sodium dextran sulfate solution; (7) A middle-aged and elderly complex inflammation animal model was obtained on the 14th day of modeling.

2. The construction method according to claim 1, characterized in that, The injection dose of D-galactose is 0.1~0.4 mL / 10 g body weight, and the molecular weight of the DSS solution is 30,000~50,000 Da.

3. The construction method according to claim 1, characterized in that, The animals were 6-week-old male BALB / c mice.

4. An animal model of complex inflammation in middle-aged and elderly individuals based on gut-liver axis dysfunction, characterized in that, The animal model is obtained by the construction method described in any one of claims 1 to 3.

5. The animal model according to claim 4, characterized in that, The animal model possesses the following characteristics: DAI A score ≥4; colon length shortening ≥20%; impaired intestinal barrier function; crypt structure destruction and inflammatory cell infiltration; significantly elevated TNF-α and IL-6 expression; decreased ZO-1 and Occludin expression; and abnormal liver oxidative stress indicators.

6. The use of the animal model described in claim 4 or 5 in screening and preparing drugs, medical nutrition products and functional foods that improve diseases related to gut-liver axis dysfunction.