Compound traditional Chinese medicine essential oil for assisting in intervention of viral pneumonia streptococcus and preparation method thereof

Abstract

This invention relates to the field of compound essential oils of traditional Chinese medicine, and particularly to a compound essential oil of traditional Chinese medicine for the adjuvant intervention against Streptococcus pneumoniae and its preparation method. The raw materials include the following by weight: 3-8 parts of patchouli essential oil; 2-6 parts of clove essential oil; 2-5 parts of costus root essential oil; 2-6 parts of nard essential oil; 3-7 parts of calamus essential oil; 1-4 parts of asarum essential oil; 3-8 parts of peppermint essential oil; 2-6 parts of artemisia essential oil; 3-7 parts of eupatorium essential oil; 45-80 parts of base oil; 2-5 parts of solubilizer; and 1-3 parts of penetration enhancer. This invention addresses the core issues of "respiratory inflammation, sputum accumulation, and decreased body defense" caused by Streptococcus pneumoniae. Through the scientific formulation of active molecules from nine kinds of Chinese herbal essential oils, a comprehensive synergistic system is formed, which has the functions of "antibacterial and antiviral, anti-inflammatory and lung-clearing, expectorant and cough-relieving, and enhanced defense." It features strong synergistic activity of active molecules, high specificity, high retention and utilization rate of active molecules, safe and convenient use, wide applicability, and simple preparation process.

Description

Technical Field

[0001] This invention relates to the field of compound essential oil technology of traditional Chinese medicine, and in particular to a compound essential oil of traditional Chinese medicine for the adjuvant intervention of Streptococcus pneumoniae and its preparation method. Background Technology

[0002] Streptococcus pneumoniae is a common respiratory pathogen that can cause various respiratory infections such as pneumonia, bronchitis, and otitis media, posing a significant threat, especially to the elderly, children, and those with weakened immune systems. This pathogen easily colonizes the respiratory mucosa, causing inflammatory reactions accompanied by symptoms such as cough, sputum production, chest tightness, and fever. In severe cases, it can lead to difficulty breathing, lung damage, and even severe infections. Clinically, antibiotics are often the primary treatment, but long-term use can lead to drug resistance and has side effects such as intestinal flora imbalance and liver and kidney damage.

[0003] Chinese herbal essential oils, as highly effective external or nebulized inhalation formulations of natural Chinese medicine, have antibacterial, antiviral, anti-inflammatory, and qi-regulating effects. Their active molecules can directly act on the respiratory mucosa, inhibiting the colonization and reproduction of pathogenic bacteria, relieving inflammatory responses, and regulating the body's respiratory defense function. They also have no risk of drug resistance and are highly safe.

[0004] Currently, most products used for adjuvant intervention against respiratory pathogens are based on single antibacterial ingredients or Western medicine preparations, which suffer from problems such as drug resistance and significant side effects. Traditional Chinese medicine (TCM) products are mostly single essential oils or simple blends, with poor synergy among the active molecules, failing to fully utilize the synergistic antibacterial, anti-inflammatory, and lung-clearing effects of multiple TCM essential oils. They also lack specificity against viral pneumonia and streptococci, resulting in limited adjuvant intervention effects. Furthermore, some TCM essential oil products suffer from low utilization rates of active molecules, complex preparation processes, and inconvenient use, hindering their widespread application.

[0005] In view of this, the development of a compound Chinese herbal essential oil based on the synergistic combination of the active molecules of nine kinds of Chinese herbal essential oils, which can be used to specifically assist in the intervention of Streptococcus pneumoniae, has significant clinical application value and industrialization prospects. Summary of the Invention

[0006] The purpose of this invention is to address the shortcomings of existing products for assisting in the intervention of Streptococcus pneumoniae, such as drug resistance, significant side effects, poor synergy of compound Chinese herbal essential oils, and weak targeting. This invention proposes a compound Chinese herbal essential oil and its preparation method for assisting in the intervention of Streptococcus pneumoniae. By scientifically combining nine kinds of Chinese herbal essential oils, including patchouli and clove, the active molecules in each essential oil achieve complementary and synergistic effects, targeting and inhibiting the colonization and reproduction of Streptococcus pneumoniae, alleviating respiratory inflammation, improving symptoms such as cough, sputum, and chest tightness, and assisting in enhancing the body's respiratory defense function, thus achieving the effect of assisting in the intervention of Streptococcus pneumoniae. Simultaneously, this invention provides a preparation method for this compound Chinese herbal essential oil. This method is simple, environmentally friendly, and can effectively retain the active molecules in each essential oil, improving the stability and efficacy of the essential oil, and facilitating industrial production and practical application.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: A compound traditional Chinese medicine essential oil for adjuvant intervention against Streptococcus pneumoniae, wherein the raw materials comprise the following by weight: Patchouli essential oil, 3-8 parts; 2-6 parts clove essential oil; 2-5 parts of woody essential oil; 2-6 parts of spikenard oil; 3-7 parts of sweet flag essential oil; 1-4 parts Asarum essential oil; 3-8 parts peppermint essential oil; 2-6 parts of mugwort essential oil; 3-7 parts of Perilla essential oil; 45-80 parts base oil; 2-5 parts of co-solvent; 1-3 parts of penetration enhancer.

[0008] As a further description of the above technical solution: The formula contains 5 parts patchouli essential oil, 4 parts clove essential oil, 3 parts costus root essential oil, 4 parts spikenard essential oil, 5 parts calamus essential oil, 2 parts asarum essential oil, 5 parts peppermint essential oil, 4 parts mugwort essential oil, 5 parts agastache essential oil, 62 parts base oil, 3 parts solubilizer, and 2 parts penetration enhancer. This ratio allows the active molecules in each herbal essential oil to achieve the best synergistic effect, taking into account the multi-faceted needs of antibacterial and antiviral, anti-inflammatory and lung-clearing, expectorant and cough-relieving properties, while ensuring the mildness and safety of the essential oils.

[0009] As a further description of the above technical solution: The essential oils mentioned above, such as patchouli oil, clove oil, costus root oil, spikenard oil, calamus oil, peppermint oil, mugwort oil, and agastache oil, are all extracted from the corresponding Chinese herbal raw materials by steam distillation.

[0010] As a further description of the above technical solution: The Asarum essential oil is made from dried Asarum whole herb through ethanol extraction and distillation.

[0011] As a further description of the above technical solution: The base oil is selected from one or more of sweet almond oil, jojoba oil, and olive oil.

[0012] As a further description of the above technical solution: The cosolvent is selected from propylene glycol and glycerin.

[0013] As a further description of the above technical solution: The penetration enhancer is selected from one of azone and menthol.

[0014] A method for preparing a compound traditional Chinese medicine essential oil for adjuvant intervention against Streptococcus pneumoniae, comprising the following steps: S100. Raw material pretreatment: Take dried patchouli, clove, costus root, nard, acorus tatarinowii, asarum, mint, artemisia argyi, and eupatorium fortunei, remove impurities, wash, dry, and pulverize to 40-60 mesh for later use; ensure that the raw materials are dry and free from mold, and that the pulverized particles are uniform in size to facilitate the full extraction of active molecules in the subsequent process. S200, Extraction of Chinese Herbal Essential Oils Separately: Extraction of Patchouli Essential Oil, Clove Essential Oil, Nardostachys Essential Oil, Acorus calamus Essential Oil, Peppermint Essential Oil, Artemisia argyi Essential Oil, and Eupatorium fortunei Essential Oil: Add the corresponding Chinese herbal powder to a distillation apparatus, soak in water for 30-50 minutes, extract by steam distillation for 3-4 hours, collect the distillate, dehydrate it with anhydrous sodium sulfate, filter it, concentrate it under reduced pressure until there is no water, and obtain the corresponding essential oil, which is then sealed for later use; Costus root essential oil extraction: Add costus root powder to a 70-80% ethanol solution with 8-10 times its weight, and reflux at 55-65℃ twice for 1.5-2 hours each time. Combine the extracts, filter, concentrate the filtrate under reduced pressure until there is no alcohol odor, and then perform steam distillation. Collect the distillate, dehydrate and filter to obtain costus root essential oil, and seal for later use. Asarum essential oil extraction: Add asarum powder to a 75-85% ethanol solution with 7-9 times its weight, soak at room temperature for 20-24 hours, stirring 3-4 times during the process, filter, concentrate the filtrate under reduced pressure until there is no alcohol odor, distill and purify, dehydrate and filter to obtain asarum essential oil, and seal for later use. S300, Mixing and Dissolving: Weigh out each herbal essential oil, cosolvent, and penetration enhancer according to the weight parts, add them to an appropriate amount of base oil, place them in a constant temperature water bath, stir at 35-45℃ for 25-35 minutes, and rotate at 180-280 r / min to fully dissolve each component and obtain a mixture; control the stirring temperature and speed to avoid high temperature damaging the active molecules and ensure that each active molecule is uniformly dissolved. S400, Homogenization: Add the remaining base oil to the above mixture and continue stirring for 20-30 minutes. Then, use a homogenizer to homogenize for 10-15 minutes at a pressure of 18-28 MPa and a temperature of 35-40°C to ensure uniform dispersion of the mixture, avoid stratification and sedimentation, and ensure uniform distribution of each active molecule. S500 Filtration and Refining: The homogenized mixture is filtered through a 0.22μm filter membrane to remove insoluble impurities and large molecular particles, resulting in a clear and transparent crude essential oil. Filtration can prevent impurities from affecting the activity of active molecules and the safety of use, ensuring the purity of the essential oil. S600, Aging and Sterilization: Place the crude essential oil in a cool and ventilated place to age for 24-48 hours, and then use intermittent ultraviolet sterilization to avoid degradation of active molecules caused by continuous sterilization, while ensuring the sterilization effect, to obtain the compound Chinese medicine essential oil product. S700 Packaging and Storage: After sterilization, the compound Chinese herbal essential oils are dispensed into sterile brown glass bottles and sealed. Store in a cool, dry, and dark place, avoiding high temperatures, direct sunlight, and violent shaking to prevent degradation of active molecules. External and atomized types are packaged separately, with instructions for use clearly marked. It is recommended to use within 6 months after opening.

[0015] As a further description of the above technical solution: In step S200, the temperature for vacuum concentration of the herbal essential oil extraction is set to 45-55℃, and the vacuum degree is maintained at -0.08~-0.06MPa. At the same time, the distillation and refining temperature for Asarum essential oil extraction is controlled at 50-55℃.

[0016] As a further description of the above technical solution: In step S600, the ultraviolet intensity for ultraviolet sterilization is 30W, the distance is controlled at 30-50cm, the sterilization time is controlled at 10min, the pause time is controlled at 5min, and the entire sterilization process is repeated twice.

[0017] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. In this invention, the active molecules exhibit strong synergy and are highly targeted: This invention scientifically combines nine kinds of Chinese herbal essential oils, and the active molecules in each essential oil complement each other and synergistically enhance each other's effects, forming a comprehensive system of action that "inhibits bacteria and viruses, reduces inflammation and clears the lungs, resolves phlegm and relieves cough, and enhances defense." It targets and inhibits the colonization and reproduction of Streptococcus pneumoniae, relieves respiratory inflammation and various discomfort symptoms, and helps enhance the body's defense function. It specifically addresses the infection problems caused by Streptococcus pneumoniae, and its effects are significantly better than single Chinese herbal essential oils or simple compound products, and there is no risk of drug resistance.

[0018] 2. In this invention, the retention rate and utilization rate of active molecules are high: Targeted extraction processes are adopted, such as steam distillation and ethanol reflux extraction, to extract the essential oils of various Chinese herbal medicines. Extraction, concentration, and sterilization parameters are strictly controlled to ensure that the retention rate of each core active molecule is ≥90%. At the same time, low-concentration penetration enhancers and mild base oils are added to significantly improve the skin and respiratory mucosa permeability and solubility of active molecules, enabling them to quickly penetrate the barrier and exert their effects, thereby improving utilization.

[0019] 3. This invention is safe, convenient, and widely applicable: It is prepared from natural Chinese herbal raw materials, without preservatives, fragrances, pigments, or other chemical additives. Combined with mild base oil, it has low skin and respiratory irritation. It can be applied to the chest or back through the skin or diluted and nebulized for inhalation. It is easy to operate and suitable for a wide range of people, including the elderly, children, and people with low immunity. It is highly safe and can be used as an adjunct therapy for a long time.

[0020] 4. The preparation process of this invention is simple and easy to promote industrially: The preparation method of this invention has clear steps and is easy to operate. It does not require complicated equipment and high costs. The extraction, mixing, homogenization and sterilization of various Chinese herbal essential oils can be carried out on a large scale. Moreover, no harmful waste is generated during the production process, which meets environmental protection requirements and is easy to promote and apply industrially. Attached Figure Description

[0021] Figure 1 This is a flowchart illustrating the process of preparing a compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae, as proposed in this invention. Figure 2 This is a real-life photo of the quantitative antibacterial experiment of compound Chinese herbal essential oils against Staphylococcus aureus suspension during the effect verification of specific embodiments of the present invention. Figure 3 This is a real-life photograph taken during the quantitative antibacterial experiment of compound Chinese herbal essential oils on Escherichia coli suspension in the specific implementation embodiment of the present invention. Figure 4 The table below shows the control of test strains used in the quantitative antibacterial experiment of compound Chinese herbal essential oil suspension for verifying the effects of specific embodiments of the present invention. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Please see Figure 1-4This invention provides a technical solution: a compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae, the raw materials comprising the following by weight: Patchouli essential oil 3-8 parts; clove essential oil 2-6 parts; costus essential oil 2-5 parts; spikenard essential oil 2-6 parts; calamus essential oil 3-7 parts; asarum essential oil 1-4 parts; peppermint essential oil 3-8 parts; wormwood essential oil 2-6 parts; eupatorium essential oil 3-7 parts; base oil 45-80 parts; solubilizer 2-5 parts; penetration enhancer 1-3 parts.

[0024] Furthermore, the preferred raw materials for the compound Chinese herbal essential oils, by weight, are: The formula consists of 5 parts patchouli essential oil, 4 parts clove essential oil, 3 parts costus root essential oil, 4 parts spikenard essential oil, 5 parts calamus essential oil, 2 parts asarum essential oil, 5 parts peppermint essential oil, 4 parts mugwort essential oil, 5 parts agastache essential oil, 62 parts base oil, 3 parts solubilizer, and 2 parts penetration enhancer. This ratio allows the active molecules in each herbal essential oil to achieve the best synergistic effect, taking into account the multi-faceted needs of antibacterial and antiviral, anti-inflammatory and lung-clearing, expectorant and cough-relieving properties, while ensuring the mildness and safety of the essential oils.

[0025] Detailed descriptions of each ingredient - focusing on active molecules and their effects: Patchouli essential oil: It is made from the dried aerial parts of patchouli through steam distillation. The core active molecules are patchouli ketone (≥0.8%) and patchouli alcohol (≥1.2%). Its core functions are aromatic dampness-removing, qi-regulating and stomach-soothing, antibacterial and antiviral. It can inhibit the reproduction of Streptococcus pneumoniae, relieve respiratory mucosal inflammation, and improve discomfort such as chest tightness, cough, and abdominal distension, laying the foundation for adjuvant intervention.

[0026] Clove essential oil: It is made from dried clove buds through steam distillation. The core active molecules are eugenol (≥8.0%) and eugenol (≥1.5%). Its effects include antibacterial and antiviral properties, warming and relieving nausea and vomiting, anti-inflammatory and analgesic effects. It has a significant inhibitory effect on Streptococcus pneumoniae, can destroy the cell membrane structure of pathogenic bacteria, and relieve respiratory inflammation and sore throat.

[0027] Costus root essential oil: It is made from dried costus root as raw material, extracted by ethanol reflux and then purified by distillation. The core active molecules are costus lactone content ≥1.0% and dehydrocostus lactone content ≥0.8%. Its effects are to regulate qi and relieve pain, strengthen the spleen and promote digestion, and assist in antibacterial activity. It can improve chest tightness and abdominal distension associated with respiratory infections, enhance the body's digestive function, and indirectly enhance the body's defense capabilities.

[0028] Nardostachys essential oil: It is made from dried Nardostachys root and rhizome through steam distillation. The core active molecules are ≥0.6% nardone and ≥0.5% valerone. Its effects include regulating qi and relieving pain, antibacterial and anti-inflammatory properties. It can help inhibit the reproduction of Streptococcus pneumoniae, relieve respiratory smooth muscle spasms, and reduce symptoms of chest tightness and cough.

[0029] Sweet flag essential oil: It is made from dried sweet flag rhizome through steam distillation. The core active molecules are α-asarone content ≥1.0% and β-asarone content ≥0.8%. Its effects include clearing the orifices and relieving phlegm, refreshing the mind and improving intelligence, and resolving phlegm and relieving cough. It can dilute phlegm, promote phlegm discharge, improve symptoms such as difficulty breathing and thick phlegm, and help relieve airway obstruction.

[0030] Asarum essential oil: It is made from dried Asarum whole herb through ethanol extraction and distillation. The core active molecules are asarone content ≥0.7% and safrole content ≤0.1%. The dosage is controlled to ensure safety. Its effects include relieving exterior syndromes and dispelling cold, dispelling wind and relieving pain, inhibiting bacteria and inflammation. It can relieve symptoms such as fever, cough and nasal congestion, and help inhibit Streptococcus pneumoniae and improve respiratory discomfort.

[0031] Peppermint essential oil: It is made from dried peppermint leaves through steam distillation. The core active molecules are menthol (≥5.0%) and menthone (≥1.0%). Its effects include dispelling wind-heat, clearing the head and eyes, and anti-inflammatory and antipruritic effects. It can relieve symptoms such as fever, sore throat, and nasal congestion, inhibit the inflammatory response of the respiratory mucosa, and enhance respiratory comfort.

[0032] Artemisia argyi essential oil: It is made from dried artemisia argyi leaves through steam distillation. The core active molecules are eucalyptol (≥1.5%) and thujone (≥0.8%). Its effects include warming the meridians and dispelling cold, antibacterial and anti-inflammatory, and enhancing immunity. It can inhibit Streptococcus pneumoniae, enhance the body's respiratory defense function, assist in regulating bodily functions, and reduce infection recurrence.

[0033] Eupatorium essential oil: It is made from the dried aerial parts of Eupatorium and extracted by steam distillation. The core active molecules are eupatorin (≥0.6%) and coumarin (≥0.5%). Its effects include aromatic dampness removal, invigorating the spleen and stomach, and assisting in antibacterial activity. It can regulate the body's spleen and stomach function, enhance the body's metabolic capacity, indirectly improve antibacterial and antiviral capabilities, and relieve loss of appetite associated with respiratory infections.

[0034] Base oil: Selected from one or more of sweet almond oil, jojoba oil, and olive oil, preferably a 1:1 mixture of sweet almond oil and jojoba oil. It has good skin permeability, moisturizing properties, and gentleness, and can serve as a carrier for the active molecules of various Chinese herbal essential oils. It is suitable for skin application or dilution and atomization for inhalation, reducing irritation to the respiratory tract and skin.

[0035] Cosolvent: Selected from propylene glycol and glycerin, with propylene glycol preferred, which can improve the solubility of various Chinese herbal essential oils in base oil, avoid the separation and precipitation of essential oils, improve the stability of essential oils, and ensure the uniform distribution of various active molecules.

[0036] Penetration enhancer: Selected from azone and menthol, with low concentration of azone preferred, which can significantly enhance the permeability of skin and respiratory mucosa, promote the rapid penetration of active molecules in various Chinese herbal essential oils to the site of action, improve the utilization rate of active molecules, and enhance the effect of auxiliary intervention.

[0037] Specifically, the synergistic effect principle of active molecules: This invention addresses the core issues of "respiratory inflammation, sputum accumulation, and decreased bodily defense" caused by Streptococcus pneumoniae. Through the scientific formulation of active molecules from nine kinds of traditional Chinese medicine essential oils, it forms a comprehensive synergistic system that "inhibits bacteria and viruses, reduces inflammation and clears the lungs, resolves phlegm and relieves cough, and enhances defense." The active molecules work together, complementing and enhancing each other's effects. The specific synergistic logic is as follows: First, synergistic antibacterial and antiviral effects: Eugenol and eugenol in clove essential oil are the core antibacterial components, which can directly inhibit the colonization and reproduction of Streptococcus pneumoniae and destroy the cell membrane structure of pathogenic bacteria; patchouli and patchouli alcohol in patchouli essential oil, nardone in spikenard essential oil, and eucalyptol in wormwood essential oil work synergistically to enhance the antibacterial effect, while also helping to inhibit respiratory viruses, reduce the risk of mixed infection, and control the progression of infection from the root. Secondly, the anti-inflammatory and lung-clearing effects are synergistic: menthol and menthone in peppermint essential oil and asarone in asarum essential oil work together to dispel wind-heat, reduce inflammation and swelling, and relieve respiratory mucosal inflammation and sore throat; costus essential oil contains costus lactone and dehydrocostus lactone to regulate qi and clear the lungs, relieve chest tightness and shortness of breath, improve respiratory ventilation function, and promote the resolution of inflammation. Third, the synergistic effect of expectoration and cough relief: α-asarone and β-asarone in Acorus tatarinowii essential oil can dilute phlegm and promote phlegm expulsion, solving the problems of thick phlegm and difficulty breathing; Patchouli essential oil and Eupatorium fortunei essential oil work together to aromatically resolve dampness, reduce phlegm production, help relieve cough and phlegm symptoms, and improve respiratory comfort; Fourth, enhance the synergistic effect of defense: the eucalyptol and thujone in Artemisia argyi essential oil and the eupatorium in Eupatorium fortunei essential oil work together to enhance the body's respiratory defense function, regulate the body's functions, improve immunity, reduce the risk of viral pneumonia and streptococcal recolonization, and help speed up the recovery process. Fifth, synergistic effect and delivery: The penetration enhancer azone works synergistically with the base oil to enhance the permeability of the skin and respiratory mucosa, promote the rapid penetration of all active molecules to the site of action, and at the same time avoid the mutual antagonism of the active molecules, so as to maximize the synergistic effect and improve the auxiliary intervention effect.

[0038] The entire compound system takes into account "antibacterial properties without damaging the body, anti-inflammatory properties without irritating the mucous membranes, and expectorant properties without depleting qi". It is mild and can be used by skin application or nebulization after dilution. It can specifically address various respiratory discomforts caused by viral pneumonia and streptococcus, and assist in infection intervention and body recovery.

[0039] Specifically, a method for preparing a compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae includes the following steps: Step 1: Raw material pretreatment: Take dried patchouli, clove, costus root, nard, acorus calamus, asarum, mint, artemisia argyi, and eupatorium, remove impurities, wash, dry, and grind to 40-60 mesh for later use; ensure that the raw materials are dry and free from mold, and that the particle size is uniform to facilitate the full extraction of active molecules in the subsequent process. Step 2: Individual extraction of essential oils from Chinese herbs: Extraction of essential oils from patchouli, clove, nard, calamus, peppermint, artemisia, and agastache: Add the corresponding Chinese herbal powders to a distillation apparatus, soak in water for 30-50 minutes, and extract by steam distillation for 3-4 hours. Collect the distillate, dehydrate it with anhydrous sodium sulfate, filter it, and concentrate it under reduced pressure until there is no water. The temperature for concentration under reduced pressure is set at 45-55℃, and the vacuum degree is maintained at -0.08~-0.06MPa. The corresponding essential oils are obtained and sealed for later use. Costus root essential oil extraction: Add costus root powder to a 70-80% ethanol solution with 8-10 times its weight, and reflux at 55-65℃ twice for 1.5-2 hours each time. Combine the extracts, filter, concentrate the filtrate under reduced pressure until there is no alcohol odor, and then perform steam distillation. Control the distillation purification temperature at 50-55℃, collect the distillate, dehydrate and filter to obtain costus root essential oil, and seal for later use. Asarum essential oil extraction: Add Asarum powder to a 75-85% ethanol solution with 7-9 times its weight, soak at room temperature for 20-24 hours, stirring 3-4 times during the process, filter, concentrate the filtrate under reduced pressure until there is no alcohol odor, distill and purify, dehydrate and filter to obtain Asarum essential oil. The safrole content should be controlled to be ≤0.1%, and sealed for later use. Step 3: Mixing and Dissolving: Weigh out each herbal essential oil, solubilizer, and penetration enhancer according to the weight proportions, add them to an appropriate amount of base oil, place them in a constant temperature water bath, stir at 35-45℃ for 25-35 minutes, and rotate at 180-280 r / min to fully dissolve each component and obtain a mixture; control the stirring temperature and speed to avoid high temperature damaging the active molecules and ensure that each active molecule is uniformly dissolved. Step 4: Homogenization: Add the remaining base oil to the above mixture and continue stirring for 20-30 minutes. Then, homogenize the mixture for 10-15 minutes using a homogenizer at a pressure of 18-28 MPa and a temperature of 35-40°C to ensure uniform dispersion of the mixture, avoid stratification and sedimentation, and ensure uniform distribution of each active molecule. Step 5, Filtration and Refining: Filter the homogenized mixture through a 0.22μm filter membrane to remove insoluble impurities and large molecular particles, obtaining a clear and transparent crude essential oil. Filtration can prevent impurities from affecting the activity of active molecules and the safety of use, ensuring the purity of the essential oil. Step 6, Aging and Sterilization: Place the crude essential oil in a cool, ventilated place to age for 24-48 hours, and then use intermittent ultraviolet sterilization. The ultraviolet intensity for ultraviolet sterilization is 30W, the distance is controlled at 30-50cm, the sterilization time is controlled at 10min, and the pause time is controlled at 5min. The entire sterilization process is repeated twice to avoid continuous sterilization leading to degradation of active molecules, while ensuring the sterilization effect, and to obtain the compound Chinese medicine essential oil product. The final step, packaging and storage: After sterilization, the compound Chinese herbal essential oils are dispensed into sterile brown glass bottles and sealed. Store them in a cool, dry, and dark place, avoiding high temperatures, direct sunlight, and violent shaking to prevent degradation of active molecules. External and atomized types are packaged separately, with instructions for use clearly marked. It is recommended to use them within 6 months after opening.

[0040] Furthermore, in step two, during the extraction of each herbal essential oil, the temperature of vacuum concentration and distillation is controlled at 45-55℃ to maximize the retention of the core active molecules in each essential oil and avoid denaturation or inactivation of active molecules due to high temperature; after extraction, the safrole content of Asarum essential oil needs to be tested to ensure ≤0.1% to ensure safe use; all essential oils need to be dehydrated after extraction to ensure that the water content of the essential oil is ≤0.5% to improve the stability of the essential oil.

[0041] Furthermore, in step four, the homogenization temperature is maintained at 35-40℃, which can improve the homogeneity and stability of the mixture, extend the shelf life of the essential oil, and at the same time avoid high temperature from damaging the active molecules.

[0042] Furthermore, in step six, the aging process allows the active molecules of each herbal essential oil to fully integrate, enhancing the synergistic effect; intermittent ultraviolet sterilization can ensure the sterilization effect while maximizing the preservation of the activity of the active molecules, avoiding the sterilization process from affecting the efficacy of the essential oil.

[0043] Example 1 A compound traditional Chinese medicine essential oil for adjuvant intervention against Streptococcus pneumoniae, the raw materials by weight are: 3 parts patchouli essential oil, 2 parts clove essential oil, 2 parts costus root essential oil, 2 parts spikenard essential oil, 3 parts calamus essential oil, 1 part asarum essential oil, 3 parts peppermint essential oil, 2 parts mugwort essential oil, 3 parts eupatorium essential oil, 45 parts base oil (choose sweet almond oil: jojoba oil = 1:1), 2 parts solubilizer (choose propylene glycol), and 1 part penetration enhancer (choose azone).

[0044] The above raw materials were prepared according to a method for preparing a compound traditional Chinese medicine essential oil for the auxiliary intervention of Streptococcus pneumoniae, wherein the controlled variables in each step are as follows: In the raw material pretreatment step, impurities are removed from the nine kinds of Chinese medicines, they are washed, dried, and pulverized to 40 mesh for later use.

[0045] In the extraction process of Chinese herbal essential oils, each essential oil is extracted according to the corresponding process described above. The extraction, concentration, and distillation temperatures are controlled at 45-55℃. The safrole content of Asarum essential oil is controlled to be ≤0.1%. After dehydration, it is sealed for later use.

[0046] In the mixing and dissolving step, weigh out each Chinese herbal essential oil, propylene glycol, and azone, add them to 8 parts of base oil, stir in a 35°C water bath for 25 minutes at a speed of 180 r / min until completely dissolved.

[0047] In the homogenization step, add the remaining 37 parts of base oil, continue stirring for 20 minutes, and then homogenize for 10 minutes at 18 MPa pressure using a homogenizer, while maintaining the temperature at 35°C.

[0048] In the aging and sterilization process, the product is aged in a cool, ventilated place for 24 hours and then sterilized using intermittent ultraviolet light to obtain the finished product.

[0049] During the packaging and storage process, dispense into sterile brown glass bottles, seal, store in a cool, dry place away from light, and label as external use only.

[0050] Example 2 (Preferred Example) A compound traditional Chinese medicine essential oil for adjuvant intervention against Streptococcus pneumoniae, the raw materials by weight are: 5 parts patchouli essential oil, 4 parts clove essential oil, 3 parts costus essential oil, 4 parts spikenard essential oil, 5 parts calamus essential oil, 2 parts asarum essential oil, 5 parts peppermint essential oil, 4 parts mugwort essential oil, 5 parts eupatorium essential oil, 62 parts base oil (using sweet almond oil: jojoba oil = 1:1), 3 parts solubilizer (using propylene glycol), and 2 parts penetration enhancer (using azone).

[0051] The above raw materials were prepared according to a method for preparing a compound traditional Chinese medicine essential oil for the auxiliary intervention of Streptococcus pneumoniae, wherein the controlled variables in each step are as follows: In the raw material pretreatment step, the nine kinds of Chinese medicines are cleaned of impurities, washed, dried, and pulverized to 50 mesh for later use.

[0052] In the extraction steps of Chinese herbal essential oils, the essential oils of patchouli, clove, nard, acorus calamus, peppermint, mugwort, and eupatorium are soaked in water for 40 minutes, steam distilled for 3.5 hours, and the distillate is dehydrated by anhydrous sodium sulfate, filtered, and concentrated under reduced pressure at 50°C until there is no water, and then set aside. Costus root essential oil: Add 9 times its weight of 75% ethanol, reflux at 60℃ twice, 1.8h each time, combine the extracts, filter, concentrate under reduced pressure until there is no alcohol taste, then steam distill, dehydrate, filter, and set aside. Asarum essential oil: Add 8 times its weight of 80% ethanol, soak at room temperature for 22 hours, stir 3 times, filter, concentrate under reduced pressure until there is no alcohol taste, distill at 52℃, dehydrate, filter, and test the safrole content to be 0.08% (meets the requirements), and set aside.

[0053] In the mixing and dissolving step, weigh out each Chinese herbal essential oil, propylene glycol, and azone, add them to 12 parts of base oil, stir in a 40°C water bath for 30 minutes at a speed of 230 r / min until completely dissolved.

[0054] In the homogenization step, add the remaining 50 parts of base oil, continue stirring for 25 minutes, and then homogenize using a homogenizer at a pressure of 23 MPa for 12 minutes while maintaining the temperature at 38°C.

[0055] During the filtration and refining process, a 0.22μm filter membrane is used to remove impurities and obtain crude essential oil.

[0056] During the sterilization process, the product is aged in a cool and ventilated place for 36 hours, and then sterilized with intermittent ultraviolet light (ultraviolet intensity 30W, distance controlled at 40cm, sterilization time controlled at 10min + pause time controlled at 5min + sterilization time controlled at 10min) to obtain the finished product.

[0057] During packaging and storage, the product is divided into external use type and nebulization type. The external use type is directly packaged, while the nebulization type is diluted with an appropriate amount of physiological saline before being packaged. Both types are sealed in sterile brown glass bottles and stored in a cool, dry place away from light.

[0058] Example 3 A compound traditional Chinese medicine essential oil for adjuvant intervention against Streptococcus pneumoniae, the raw materials by weight are: 8 parts patchouli essential oil, 6 parts clove essential oil, 5 parts costus essential oil, 6 parts spikenard essential oil, 7 parts calamus essential oil, 4 parts asarum essential oil, 8 parts peppermint essential oil, 6 parts mugwort essential oil, 7 parts eupatorium essential oil, 80 parts base oil (using sweet almond oil:jojoba oil = 1:1), 5 parts solubilizer (using propylene glycol), and 3 parts penetration enhancer (using azone).

[0059] The above raw materials were prepared according to a method for preparing a compound traditional Chinese medicine essential oil for the auxiliary intervention of Streptococcus pneumoniae, wherein the controlled variables in each step are as follows: In the raw material pretreatment step, the nine kinds of Chinese medicines are cleaned of impurities, washed, dried, and pulverized to 60 mesh for later use.

[0060] In the extraction process of Chinese herbal essential oils, each essential oil is extracted according to the corresponding process described above. The extraction, concentration, and distillation temperatures are controlled at 50-55℃. The safrole content of Asarum essential oil is controlled to be ≤0.1%. After dehydration, it is sealed for later use.

[0061] In the mixing and dissolving step, weigh out each Chinese herbal essential oil, propylene glycol, and azone, add them to 15 parts of base oil, stir in a 45°C water bath for 35 minutes at a speed of 280 r / min until completely dissolved.

[0062] In the blending and homogenization step, add the remaining 65 parts of base oil, continue stirring for 30 minutes, and then homogenize using a homogenizer at a pressure of 28 MPa for 15 minutes while maintaining the temperature at 40°C.

[0063] In the filtration and refining step, a 0.22μm filter membrane is used to remove impurities and obtain crude essential oil.

[0064] In the aging and sterilization process, the product is aged in a cool, ventilated place for 48 hours and then sterilized using intermittent ultraviolet light to obtain the finished product.

[0065] During the packaging and storage process, dispense into sterile brown glass bottles, seal, store in a cool, dry place away from light, and label the concentration to be used.

[0066] Effect verification For details, please refer to Figure 2 , Figure 3 and Figure 4 And as shown in Table 1 below, quantitative suspension inhibition experiments were also conducted on the experimental strains.

[0067] Experimental objective: To quantitatively evaluate the antibacterial effects of compound Chinese herbal essential oils on Escherichia coli, Staphylococcus aureus, and Streptococcus pneumoniae, and to quantify efficacy by calculating the antibacterial rate or bactericidal rate.

[0068] Preparation of bacterial suspension: Take a 24-hour fresh culture of Streptococcus pneumoniae and dilute it with PBS buffer to 5.0 × 10⁻⁶. 5 ~5.0×10 6 CFU / mL concentration. Ensure the strain is in the logarithmic growth phase to maintain activity.

[0069] Sample preparation: Dilute the sample to be tested (e.g., antibacterial agent) to the working concentration according to the instructions and equilibrate in a 20℃±1℃ water bath for 5 minutes. Use PBS as a positive control instead of the sample, and sterile PBS + culture medium for mixing and reaction. Mixing and reaction: Mix 5 mL of sample with 0.1 mL of bacterial suspension and start timing immediately. Set the reaction time according to the product instructions (usually 5~60 minutes), and control the temperature at 20℃±1℃.

[0070] Viable cell count and result calculation: After the treatment, inoculate 1 mL of the mixture into two Petri dishes and pour in agar medium (such as tryptone-soy agar). Incubate at 37°C for 48 hours (for bacterial vegetative cells), then count the colonies. Formula for calculating the inhibition rate: Antibacterial rate = 1 - (Average colony count in positive control group / Average colony count in experimental group) x 100% Quality control requirements: The recovered bacterial count for the positive control must be 1.0 × 10⁻⁶. 4 ~9.0×10 4 Within the CFU / mL range; otherwise, the experiment is invalid. For repeatability, the experiment must be independently repeated three times, and the average value should be taken to improve reliability.

[0071] Table 1: Quantitative Antibacterial Experiment of Suspension ; To further verify whether the prepared compound Chinese herbal essential oil has an auxiliary intervention effect on Streptococcus pneumoniae, four bottles of the compound Chinese herbal essential oil prepared in Example 2 were selected and sent for testing. The Guangdong Provincial Center for Microbiology Analysis and Testing conducted a quantitative suspension antibacterial experiment. The report number is 2023SP00274R01, and the official website of the test report is www.gddcm.com. The test conclusion is that the original sample solution, after 18 hours of action, showed an inhibition rate of >99.99% against Streptococcus pneumoniae, which meets the result judgment of WS / T650-2019 (5.1.1.5) (inhibition rate ≥90%, indicating strong antibacterial effect). The sample has a strong antibacterial effect against the tested strain.

[0072] In summary, the compound Chinese medicine essential oil prepared according to the method for assisting in the intervention of Streptococcus pneumoniae has a strong antibacterial effect against Escherichia coli, Staphylococcus aureus and Streptococcus pneumoniae.

[0073] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A compound traditional Chinese medicine essential oil for adjuvant intervention against Streptococcus pneumoniae, characterized in that, The raw materials, by weight, include the following: Patchouli essential oil, 3-8 parts; 2-6 parts clove essential oil; 2-5 parts of woody essential oil; 2-6 parts of spikenard oil; 3-7 parts of sweet flag essential oil; 1-4 parts Asarum essential oil; 3-8 parts peppermint essential oil; 2-6 parts of mugwort essential oil; 3-7 parts of Perilla essential oil; 45-80 parts base oil; 2-5 parts of co-solvent; 1-3 parts of penetration enhancer.

2. The compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 1, characterized in that, The formula contains 5 parts patchouli essential oil, 4 parts clove essential oil, 3 parts costus root essential oil, 4 parts spikenard essential oil, 5 parts calamus essential oil, 2 parts asarum essential oil, 5 parts peppermint essential oil, 4 parts mugwort essential oil, 5 parts agastache essential oil, 62 parts base oil, 3 parts solubilizer, and 2 parts penetration enhancer. This ratio allows the active molecules in each herbal essential oil to achieve the best synergistic effect, taking into account the multi-faceted needs of antibacterial and antiviral, anti-inflammatory and lung-clearing, expectorant and cough-relieving properties, while ensuring the mildness and safety of the essential oils.

3. A compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 1 or 2, characterized in that, The essential oils mentioned above, such as patchouli oil, clove oil, costus root oil, spikenard oil, calamus oil, peppermint oil, mugwort oil, and agastache oil, are all extracted from the corresponding Chinese herbal raw materials by steam distillation.

4. The compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 1, characterized in that, The Asarum essential oil is made from dried Asarum whole herb through ethanol extraction and distillation.

5. The compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 1, characterized in that, The base oil is selected from one or more of sweet almond oil, jojoba oil, and olive oil.

6. The compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 1, characterized in that, The cosolvent is selected from propylene glycol and glycerin.

7. The compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 1, characterized in that, The penetration enhancer is selected from one of azone and menthol.

8. The method for preparing a compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 1, characterized in that, The steps include the following: S100. Raw material pretreatment: Take dried patchouli, clove, costus root, nard, acorus tatarinowii, asarum, mint, artemisia argyi, and eupatorium fortunei, remove impurities, wash, dry, and pulverize to 40-60 mesh for later use; ensure that the raw materials are dry and free from mold, and that the pulverized particles are uniform in size to facilitate the full extraction of active molecules in the subsequent process. S200, Extraction of Chinese Herbal Essential Oils Separately: Extraction of Patchouli Essential Oil, Clove Essential Oil, Nardostachys Essential Oil, Acorus calamus Essential Oil, Peppermint Essential Oil, Artemisia argyi Essential Oil, and Eupatorium fortunei Essential Oil: Add the corresponding Chinese herbal powder to a distillation apparatus, soak in water for 30-50 minutes, extract by steam distillation for 3-4 hours, collect the distillate, dehydrate it with anhydrous sodium sulfate, filter it, concentrate it under reduced pressure until there is no water, and obtain the corresponding essential oil, which is then sealed for later use; Costus root essential oil extraction: Add costus root powder to a 70-80% ethanol solution with 8-10 times its weight, and reflux at 55-65℃ twice for 1.5-2 hours each time. Combine the extracts, filter, concentrate the filtrate under reduced pressure until there is no alcohol odor, and then perform steam distillation. Collect the distillate, dehydrate and filter to obtain costus root essential oil, and seal for later use. Asarum essential oil extraction: Add asarum powder to a 75-85% ethanol solution with 7-9 times its weight, soak at room temperature for 20-24 hours, stirring 3-4 times during the process, filter, concentrate the filtrate under reduced pressure until there is no alcohol odor, distill and purify, dehydrate and filter to obtain asarum essential oil, and seal for later use. S300, Mixing and Dissolving: Weigh out each herbal essential oil, cosolvent, and penetration enhancer according to the weight parts, add them to an appropriate amount of base oil, place them in a constant temperature water bath, stir at 35-45℃ for 25-35 minutes, and rotate at 180-280 r / min to fully dissolve each component and obtain a mixture; control the stirring temperature and speed to avoid high temperature damaging the active molecules and ensure that each active molecule is uniformly dissolved. S400, Homogenization: Add the remaining base oil to the above mixture and continue stirring for 20-30 minutes. Then, use a homogenizer to homogenize for 10-15 minutes at a pressure of 18-28 MPa and a temperature of 35-40°C to ensure uniform dispersion of the mixture, avoid stratification and sedimentation, and ensure uniform distribution of each active molecule. S500, Filtration and Refining: The homogenized mixture is filtered through a 0.22μm filter membrane to remove insoluble impurities and large molecular particles, resulting in a clear and transparent crude essential oil. Filtration prevents impurities from affecting the activity of active molecules and ensures the safety of use, thus guaranteeing the purity of essential oils; S600, Aging and Sterilization: Place the crude essential oil in a cool and ventilated place to age for 24-48 hours, and then use intermittent ultraviolet sterilization to avoid degradation of active molecules caused by continuous sterilization, while ensuring the sterilization effect, to obtain the compound Chinese medicine essential oil product. S700 Packaging and Storage: After sterilization, the compound Chinese herbal essential oils are dispensed into sterile brown glass bottles and sealed. Store in a cool, dry, and dark place, avoiding high temperatures, direct sunlight, and violent shaking to prevent degradation of active molecules. External and atomized types are packaged separately, with instructions for use clearly marked. It is recommended to use within 6 months after opening.

9. The method for preparing a compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 8, characterized in that, In step S200, the temperature for vacuum concentration of the herbal essential oil extraction is set to 45-55℃, and the vacuum degree is maintained at -0.08~-0.06MPa. At the same time, the distillation and refining temperature for Asarum essential oil extraction is controlled at 50-55℃.

10. The method for preparing a compound traditional Chinese medicine essential oil for assisting in the intervention of Streptococcus pneumoniae according to claim 8, characterized in that, In step S600, the ultraviolet intensity for ultraviolet sterilization is 30W, the distance is controlled at 30-50cm, the sterilization time is controlled at 10min, the pause time is controlled at 5min, and the entire sterilization process is repeated twice.

Images