Herb extract-based wound repair spray and method of making same
By using a compound spray of broom leaf extract, neohesperidin, scopolamine hydrobromide, and other herbal extracts, the problem of poor stability in herbal wound repair preparations has been solved, achieving rapid and efficient wound repair.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- 江苏亨瑞生物医药科技有限公司
- Filing Date
- 2026-05-09
- Publication Date
- 2026-06-09
Smart Images
Figure SMS_1
Abstract
Description
Technical Field
[0001] This invention relates to the technical field of wound care spray formulations, and provides a wound repair spray based on herbal extracts and its preparation method. Background Technology
[0002] There are many scenarios that can cause wounds, such as accidental abrasions, scratches, and cuts in daily life; burns; incisions and sutures from various surgeries; skin ulcers and chronic, slow-healing wounds caused by circulatory disorders, neuropathy, or infection; and skin infections and damage following eczema, dermatitis, herpes, and insect bites. The use of wound repair agents can help prevent wound infection, shorten healing time, reduce exudation, stop bleeding, astringe, relieve pain, and reduce scar formation.
[0003] Common types of wound healing preparations include sprays, gels, ointments / creams, film-forming agents, and dressings. Classified by the nature of their active ingredients, they can be categorized into chemical drugs (such as povidone-iodine, chlorhexidine, antibiotics, and nonsteroidal anti-inflammatory drugs), biological products (such as collagen, chitosan, enzyme preparations, and hyaluronic acid), herbal medicines (i.e., natural medicines derived from plants, animals, and minerals), and compound preparations (combinations of two or more drugs with different properties, such as traditional Chinese medicine + chemical drugs, or traditional Chinese medicine + biological products).
[0004] The advantages of using herbal medicines for wound repair are: First, the synergistic effect of multiple components is more comprehensive than that of a single component, and it is applicable to a wider range of wounds. For example, when a traditional Chinese medicine contains flavonoids, polysaccharides, and polyphenols, it can have multiple effects such as anti-inflammatory and antibacterial properties, hemostasis, astringency, and promoting healing. Second, it is derived from natural substances, is mild, low in irritation, and has a high safety profile. Third, it acts on multiple targets, is less likely to cause drug resistance and allergic reactions, and is suitable for long-term use.
[0005] However, herbal wound repair preparations also have some drawbacks. For example, the effects of herbal medicines are relatively mild and slow to take effect, and the efficacy needs to be improved. In addition, due to the poor stability of the extract, it is easy to oxidize, discolor, and separate into layers and precipitate, which will also lead to a decrease in efficacy. Summary of the Invention
[0006] In view of the above situation, the present invention proposes a wound repair spray based on herbal extract and its preparation method, which can overcome the disadvantage of poor stability of the extract and obtain a fast and efficient herbal wound repair preparation.
[0007] The specific technical solution involved in this invention is as follows: The first part of the present invention is to provide a wound repair spray based on herbal extracts, the components of which include Bletilla striata extract, Ampelopsis japonica extract, Sanguisorba officinalis extract, Phellodendron chinense extract, Scutellaria baicalensis extract, Polygonum cuspidatum extract, Platycladus orientalis leaf extract, glycerin, and extract of Broomella vulgaris leaf / neohesperidin / hyoscyamine hydrobromide.
[0008] The preparation process of the extract of *Broomia spp.* leaf / neohesperidin / hyoscyamine hydrobromide is as follows: S1. Dissolve 0.08-0.12 parts by weight of neohesperidin in 70-80 parts by weight of sodium bicarbonate aqueous solution (pH=8-9) to prepare solution x; S2. Dissolve 0.01-0.02 parts by weight of scopolamine in 20-30 parts by weight of ethanol, adjust the pH to 8-9, and prepare solution y; S3. Mix solution x and solution y evenly, add 3-8 parts by weight of fine powdered leaves of broom flower, and heat and sonicate. S4. Filter and remove ethanol by vacuum distillation; S5. Add dilute HBr dropwise to adjust the pH to 6-6.5.
[0009] Preferably, the particle size of the fine powder from the leaves of the broom flower is 80-120 mesh.
[0010] Preferably, the temperature for ultrasonic treatment is 40-50℃ and the time is 10-40 minutes.
[0011] Preferably, the concentration of dilute HBr is 0.05-0.2 mol / L.
[0012] Extracts from the leaves of *Broomria fusiforme* effectively inhibit microbial growth at wound sites and alleviate oxidative stress by reducing lipid peroxidation and activating antioxidant enzymes, thus accelerating wound healing. The preparation of the above extract involved extracting fine powder from *Broomria fusiforme* leaves using an alcohol / water system, with the addition of neohesperidin and anisodamine. The weakly alkaline environment not only facilitates the dissolution of active ingredients but also promotes the dissolution of neohesperidin in the extraction system and allows anisodamine to exist in a free alkali form. During extraction, neohesperidin, with its strong antioxidant capacity, inhibits oxidation during the extraction process, reducing the loss of active ingredients. Its amphiphilic nature (lipophilic flavonoid ring, hydrophilic glycosyl group) also contributes to the solubility of active ingredients, and the glycosyl group improves the hydrophilicity of the system, making the extract more stable and uniform, preventing precipitation or stratification. After extraction, neohesperidin remains in the extract and effectively scavenge reactive oxygen species, inhibiting oxidative stress and breaking the vicious cycle of "ROS-inflammation," thus eliminating the need for removal. It can work together with the active ingredients extracted from *Broomria fusiforme* leaves to promote wound healing.
[0013] This invention first adds the free scopolamine alkaloid to an alkaline alcohol / water extract of *Broomia oleracea* leaf extract and neohesperidin. After extraction, dilute HBr is added dropwise to adjust the system from weakly alkaline to weakly acidic. Simultaneously, the free scopolamine alkaloid is converted into scopolamine hydrobromide in situ. The free scopolamine alkaloid itself has extremely high solubility in an ethanol / water mixture and is perfectly miscible with *Broomia oleracea* leaf extract and neohesperidin. However, if scopolamine hydrobromide is added directly to an alkaline solution, a precipitate will form. Therefore, the in-situ salting method provides excellent miscibility during extraction, ensuring a clear and transparent extract. After extraction, the solution becomes weakly acidic, making it gentle on the skin and less irritating to wounds. The salted scopolamine hydrobromide is more stable and, through multi-target synergistic action, accelerates wound healing from four dimensions: microcirculation, inflammation, pain, and tissue repair. The main effects of broom flower leaf extract are anti-inflammatory, antibacterial, and astringent. Neohesperidin has the main effects of antioxidant, anti-inflammatory, and healing-promoting. Scopolamine hydrobromide has the main effects of relieving smooth muscle spasms, improving microcirculation, and relieving pain. It can dilate blood vessels and improve local blood flow, providing a delivery channel for the anti-inflammatory, astringent, and tissue-regenerating components of neohesperidin and broom flower leaf extract, improving local absorption efficiency, and forming a multi-closed loop of antioxidant, anti-inflammatory, blood-activating, and repairing effects.
[0014] Preferably, the components of the spray are in the following weight proportions: 0.1-0.2 parts of Bletilla striata extract, 0.05-0.1 parts of Ampelopsis japonica extract, 0.05-0.1 parts of Sanguisorba officinalis extract, 0.08-0.12 parts of Phellodendron chinense extract, 0.1-0.15 parts of Scutellaria baicalensis extract, 0.05-0.1 parts of Polygonum multiflorum extract, 0.03-0.08 parts of Platycladus orientalis leaf extract, 2-4 parts of glycerin, and 100 parts of extract of Broomsia bicolor leaf / neohesperidin / hyoscyamine hydrobromide.
[0015] Bletilla striata, Ampelopsis japonica, Sanguisorba officinalis, Scutellaria baicalensis, and Polygonum aviculare are all natural herbal plants. Phellodendron chinense and Platycladus orientalis leaves are also commonly used natural medicinal plants. Combining these component extracts with extracts of Cyperus rotundus leaves / neohesperidin / hyoscyamine hydrobromide can not only utilize the anti-inflammatory, antibacterial, antioxidant, hemostatic, and astringent effects of these components, but also promote film formation, adhesion and protection of wounds, and clear heat and dry dampness.
[0016] The second part of this invention provides a method for preparing the above-mentioned wound repair spray based on herbal extracts, comprising the following steps: B1. Divide the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide into two parts, a and b, with part a accounting for 10-20%. B2. Heat part a to 45-50℃, slowly add Bletilla striata extract, and stir to obtain gel a; B3. Heat part b to 45-50℃, add glycerin and stir, then add the extracts of Ampelopsis japonica, Sanguisorba officinalis, Phellodendron chinense, Scutellaria baicalensis, Polygonum cuspidatum and Platycladus orientalis, and stir to obtain solution b. B4. Add the adhesive solution a to the solution b while stirring, and finally adjust the pH to 6-6.5.
[0017] This invention provides a wound repair spray based on herbal extracts and its preparation method. The beneficial effects are as follows: The invention first uses an alkaline alcohol / aqueous solution of *Broomia spp.* leaf extract, neohesperidin, and anisodamine as the extraction system. The extraction is performed by heating and ultrasonic extraction. After extraction, dilute HBr is added dropwise to adjust the pH to weakly acidic, yielding an extract of *Broomia spp.* leaf extract, neohesperidin, and anisodamine hydrobromide. This extract is then compounded with extracts of *Bletilla striata*, *Ampelopsis japonica*, *Sanguisorba officinalis*, *Phellodendron chinense*, *Scutellaria baicalensis*, *Polygonum cuspidatum*, *Platycladus orientalis* leaf extract, and glycerin to obtain the wound repair spray. During the extraction process, the extract system is stable, homogeneous, clear, and transparent, without precipitation or stratification. The final wound repair spray is gentle on the skin and has minimal irritation to wounds. Through the synergistic effect of *Broomia spp.* leaf extract, neohesperidin, and anisodamine hydrobromide, a multi-layered closed loop of antioxidant, anti-inflammatory, blood-activating, and repairing effects is formed. Combined with the compounding of natural plant medicines, it can achieve rapid and efficient wound repair. Detailed Implementation
[0018] The following embodiments are preferred embodiments and effect verifications of the technical solutions of the present invention, and do not constitute a limitation on the scope of protection.
[0019] Example 1:
[0020] Raw material introduction: The extracts of Bletilla striata, Ampelopsis japonica, Sanguisorba officinalis, Phellodendron chinense, Scutellaria baicalensis, Polygonum multiflorum, and Platycladus orientalis leaf are all commercially available products. The extracts used in this embodiment and the following embodiments are all from Lanzhou Waterles Biotechnology Co., Ltd. The fine powder of Broom's leaves was prepared in-house by washing fresh leaves, air-drying them, and grinding them into a fine powder with a particle size of 80-120 mesh. Neohesperidin was from Baoji Fangcheng Biotechnology Development Co., Ltd. Scopolamine was from Weikeqi Biotechnology Co., Ltd. The concentration of dilute HBr was 0.1 mol / L.
[0021] The preparation process of the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide is as follows: S1. Dissolve 0.09 parts by weight of neohesperidin in 75 parts by weight of sodium bicarbonate aqueous solution (pH=8.5) to obtain solution x; S2. Dissolve 0.01 parts by weight of scopolamine in 25 parts by weight of ethanol, adjust the pH to 8.5, and obtain solution y; S3. Mix solution x and solution y evenly, add 4 parts by weight of fine powdered leaves of broom flower, and sonicate at 50℃ for 20 min; during the ultrasonic extraction process, the extract remained clear and no precipitate was produced. S4. Filter and remove ethanol by vacuum distillation; S5. Add dilute HBr dropwise to adjust the pH to 6.
[0022] The preparation process of the wound healing spray is as follows: B1. Divide the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide into two parts, a and b, with part a accounting for 10%. B2. Heat part a to 48°C, slowly add Bletilla striata extract, and stir to obtain gel a; B3. Heat part b to 48°C, add glycerin and stir, then add the extracts of Ampelopsis japonica, Sanguisorba officinalis, Phellodendron chinense, Scutellaria baicalensis, Polygonum cuspidatum and Platycladus orientalis, and stir to obtain solution b. B4. Add the adhesive solution a to the solution b while stirring, and finally adjust the pH to 6.
[0023] The weight parts of each component are as follows: 0.13 parts of Bletilla striata extract, 0.09 parts of Ampelopsis japonica extract, 0.06 parts of Sanguisorba officinalis extract, 0.08 parts of Phellodendron chinense extract, 0.15 parts of Scutellaria baicalensis extract, 0.07 parts of Polygonum multiflorum extract, 0.07 parts of Platycladus orientalis leaf extract, 3 parts of glycerin, and 100 parts of extract of Broomella vulgaris leaf / neohesperidin / hyoscyamine hydrobromide.
[0024] Example 2:
[0025] The preparation process of the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide is as follows: S1. Dissolve 0.09 parts by weight of neohesperidin in 75 parts by weight of sodium bicarbonate aqueous solution (pH=8) to prepare solution x; S2. Dissolve 0.015 parts by weight of scopolamine in 25 parts by weight of ethanol, adjust the pH to 8, and prepare solution y; S3. Mix solution x and solution y evenly, add 5 parts by weight of fine powdered leaves of broom flower, and sonicate at 48℃ for 25 min; during the ultrasonic extraction process, the extract remains clear and no precipitate is produced; S4. Filter and remove ethanol by vacuum distillation; S5. Add dilute HBr dropwise to adjust the pH to 6.5.
[0026] The preparation process of the wound healing spray is as follows: B1. Divide the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide into two parts, a and b, with part a accounting for 15%. B2. Heat part a to 50°C, slowly add Bletilla striata extract, and stir to obtain gel solution a; B3. Heat part b to 50°C, add glycerin and stir, then add the extracts of Ampelopsis japonica, Sanguisorba officinalis, Phellodendron chinense, Scutellaria baicalensis, Polygonum cuspidatum and Platycladus orientalis, and stir to obtain solution b. B4. Add the adhesive solution a to the solution b while stirring, and finally adjust the pH to 6.5.
[0027] The weight parts of each component are as follows: 0.15 parts of Bletilla striata extract, 0.08 parts of Ampelopsis japonica extract, 0.08 parts of Sanguisorba officinalis extract, 0.12 parts of Phellodendron chinense extract, 0.1 parts of Scutellaria baicalensis extract, 0.07 parts of Polygonum multiflorum extract, 0.05 parts of Platycladus orientalis leaf extract, 3 parts of glycerin, and 100 parts of extract of Broomella vulgaris leaf / neohesperidin / hyoscyamine hydrobromide.
[0028] Example 3:
[0029] The preparation process of the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide is as follows: S1. Dissolve 0.1 parts by weight of neohesperidin in 75 parts by weight of sodium bicarbonate aqueous solution (pH=8.5) to obtain solution x; S2. Dissolve 0.015 parts by weight of scopolamine in 25 parts by weight of ethanol, adjust the pH to 8.5, and obtain solution y; S3. Mix solution x and solution y evenly, add 6 parts by weight of fine powdered leaves of broom flower, and sonicate at 46℃ for 30 min; during the ultrasonic extraction process, the extract remains clear and no precipitate is produced; S4. Filter and remove ethanol by vacuum distillation; S5. Add dilute HBr dropwise to adjust the pH to 6.5.
[0030] The preparation process of the wound healing spray is as follows: B1. Divide the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide into two parts, a and b, with part a accounting for 20%. B2. Heat part a to 50°C, slowly add Bletilla striata extract, and stir to obtain gel solution a; B3. Heat part b to 50°C, add glycerin and stir, then add the extracts of Ampelopsis japonica, Sanguisorba officinalis, Phellodendron chinense, Scutellaria baicalensis, Polygonum cuspidatum and Platycladus orientalis, and stir to obtain solution b. B4. Add the adhesive solution a to the solution b while stirring, and finally adjust the pH to 6.5.
[0031] The weight parts of each component are as follows: 0.17 parts of Bletilla striata extract, 0.05 parts of Ampelopsis japonica extract, 0.05 parts of Sanguisorba officinalis extract, 0.1 parts of Phellodendron chinense extract, 0.1 parts of Scutellaria baicalensis extract, 0.1 parts of Polygonum multiflorum extract, 0.08 parts of Platycladus orientalis leaf extract, 3 parts of glycerin, and 100 parts of extract of Broomella vulgaris leaf / neohesperidin / hyoscyamine hydrobromide.
[0032] Example 4:
[0033] The preparation process of the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide is as follows: S1. Dissolve 0.11 parts by weight of neohesperidin in 75 parts by weight of sodium bicarbonate aqueous solution (pH=8) to prepare solution x; S2. Dissolve 0.02 parts by weight of scopolamine in 25 parts by weight of ethanol, adjust the pH to 8, and prepare solution y; S3. Mix solution x and solution y evenly, add 7 parts by weight of fine powdered leaves of broom flower, and sonicate at 45℃ for 35 min; during the ultrasonic extraction process, the extract remains clear and no precipitate is produced; S4. Filter and remove ethanol by vacuum distillation; S5. Add dilute HBr dropwise to adjust the pH to 6.
[0034] The preparation process of the wound healing spray is as follows: B1. Divide the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide into two parts, a and b, with part a accounting for 10%. B2. Heat part a to 45°C, slowly add Bletilla striata extract, and stir to obtain gel a; B3. Heat part b to 45°C, add glycerin and stir, then add the extracts of Ampelopsis japonica, Sanguisorba officinalis, Phellodendron chinense, Scutellaria baicalensis, Polygonum cuspidatum and Platycladus orientalis, and stir to obtain solution b. B4. Add the adhesive solution a to the solution b while stirring, and finally adjust the pH to 6.
[0035] The weight parts of each component are as follows: 0.15 parts of Bletilla striata extract, 0.1 parts of Ampelopsis japonica extract, 0.07 parts of Sanguisorba officinalis extract, 0.12 parts of Phellodendron chinense extract, 0.12 parts of Scutellaria baicalensis extract, 0.05 parts of Polygonum multiflorum extract, 0.04 parts of Platycladus orientalis leaf extract, 3 parts of glycerin, and 100 parts of extract of Broomella vulgaris leaf / neohesperidin / hyoscyamine hydrobromide.
[0036] Test Example 1: Four SD rats (weighing 200-250g) were used. Their backs were shaved and disinfected. Two symmetrical full-thickness wounds were created using an 8mm punch, removing the epidermis and dermis down to the subcutaneous fascia. Each rat was divided into a control group and an experimental group, one on each side. The control group received physiological saline without active ingredients, while the experimental group received the sprays described in Examples 1-4. Application method: Spray evenly onto the wound twice daily, 0.15mL / cm² each time. 2 On day 7, the wound was photographed, and the area was measured using ImageJ software. The 7-day wound closure rate was calculated as: (initial wound area - wound area at test) × 100% / initial wound area. The number of days with 100% epithelialization, no exudation, and no redness or swelling was recorded as the complete healing time. The experimental results are shown in Table 1.
[0037] As shown in Table 1, calculations based on the 7-day wound closure rate data show that, compared to using saline, the sprays from Examples 1-4 increased the 7-day wound closure rate by 103.7% (calculated as 65.4 / 32.1), 116.2% (calculated as 67.9 / 31.4), 123.9% (calculated as 71.2 / 31.8), and 130.3% (calculated as 74.4 / 32.3), respectively. Calculations based on the complete healing time data show that, compared to using saline, the sprays from Examples 1-4 shortened the complete healing time by 4 days (calculated as 17-13), 5 days (calculated as 18-13), 6 days (calculated as 18-12), and 7 days (calculated as 17-10), respectively. Therefore, the wound repair spray of this invention can significantly promote wound closure and shorten healing time, i.e., it can achieve rapid and efficient wound repair.
[0038] Table 1:
Claims
1. A wound-healing spray based on herbal extracts, the spray comprising Bletilla striata extract, Ampelopsis japonica extract, Sanguisorba officinalis extract, Phellodendron chinense extract, Scutellaria baicalensis extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and glycerin, characterized in that, It also includes extracts of broom leaves / neohesperidin / hyoscyamine hydrobromide.
2. The wound healing spray according to claim 1, characterized in that, The preparation process of the extract of *Broomia spp.* leaf / neohesperidin / hyoscyamine hydrobromide is as follows: S1. Dissolve 0.08-0.12 parts by weight of neohesperidin in 70-80 parts by weight of sodium bicarbonate aqueous solution (pH=8-9) to prepare solution x; S2. Dissolve 0.01-0.02 parts by weight of scopolamine in 20-30 parts by weight of ethanol, adjust the pH to 8-9, and prepare solution y; S3. Mix solution x and solution y evenly, add 3-8 parts by weight of fine powdered leaves of broom flower, and heat and sonicate. S4. Filter and remove ethanol by vacuum distillation; S5. Add dilute HBr dropwise to adjust the pH to 6-6.
5.
3. The wound healing spray according to claim 2, characterized in that, The fine powder from the leaves of the broom flower has a particle size of 80-120 mesh.
4. The wound healing spray according to claim 2, characterized in that, The temperature for ultrasonic treatment is 40-50℃, and the time is 10-40 minutes.
5. The wound healing spray according to claim 2, characterized in that, The concentration of dilute HBr is 0.05-0.2 mol / L.
6. The wound healing spray according to claim 1, characterized in that, The weight parts of each component are as follows: Bletilla striata extract 0.1-0.2 parts, Ampelopsis japonica extract 0.05-0.1 parts, Sanguisorba officinalis extract 0.05-0.1 parts, Phellodendron chinense extract 0.08-0.12 parts, Scutellaria baicalensis extract 0.1-0.15 parts, Polygonum aviculare extract 0.05-0.1 parts, Platycladus orientalis leaf extract 0.03-0.08 parts, Glycerin 2-4 parts, and 100 parts of extract of Broomcornu leaf / neohesperidin / hyoscyamine hydrobromide.
7. A method for preparing the wound healing spray according to any one of claims 1-6, characterized in that, Includes the following steps: B1. Divide the extract of *Gnaphalium affine* leaves / neohesperidin / hyoscyamine hydrobromide into two parts, a and b, with part a accounting for 10-20%. B2. Heat part a to 45-50℃, slowly add Bletilla striata extract, and stir to obtain gel a; B3. Heat part b to 45-50℃, add glycerin and stir, then add the extracts of Ampelopsis japonica, Sanguisorba officinalis, Phellodendron chinense, Scutellaria baicalensis, Polygonum cuspidatum and Platycladus orientalis, and stir to obtain solution b. B4. Add the adhesive solution a to the solution b while stirring, and finally adjust the pH to 6-6.5.