Pharmacognosy composition with auxiliary hypoglycemic and fat-reducing functions, fermentation liquor and preparation method and application thereof

Abstract

The application discloses a kind of medicine-food homologous compositions with the function of auxiliary reducing blood sugar and reducing fat, fermentation liquor and its preparation method and application.The application uses radix codonopsis, malt, lotus leaf, white kidney bean, hawthorn, radix puerariae as medicine-food homologous food material raw materials, mixed in proportion after water extraction, form medicine-food homologous composition with good function of reducing blood sugar and reducing fat, then form medicine-food homologous composition fermentation liquor with further improved function of reducing blood sugar and reducing fat after mixed bacteria fermentation.The application enhances the bioavailability of medicine-food homologous food material by microbial fermentation technology, and the prepared medicine-food homologous composition and fermentation liquor can be applied to the development of auxiliary reducing blood sugar and reducing fat functional products.

Description

Technical Field

[0001] This invention relates to a food-medicine homologous composition with auxiliary blood sugar and lipid reduction functions, a fermentation broth, its preparation method and application, belonging to the field of microbial fermentation. Background Technology

[0002] Food and medicine homology substances can be consumed as food or medicine to alleviate diseases with almost no toxic side effects, and they also have strong regenerative abilities and are abundant. Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root are considered food and medicine homology ingredients in the *Pharmacopoeia of the People's Republic of China*. In recent years, Codonopsis pilosula has been found to have good efficacy in treating hypotension, hyperlipidemia, coronary heart disease, and dysfunctional uterine bleeding. Lotus leaf has a wide range of pharmacological activities, including antioxidant, antibacterial, anti-obesity, and anti-cardiovascular disease activities. Barley malt is rich in various bioactive components, such as phenols, maltine, and β-glucan, and has antioxidant, laxative, and spleen-strengthening effects. White kidney bean is widely recognized as a plant-based food with high nutritional value. Its rich protein, dietary fiber, and various minerals, such as calcium, iron, and potassium, are effective in improving digestive system function, regulating blood sugar, and lowering blood lipid levels, and are particularly prominent in weight loss and diabetes management. Hawthorn has the effects of preventing cardiovascular diseases, lowering blood pressure, protecting the retina, relieving asthma, inhibiting bacteria, enhancing heart function, increasing coronary blood flow, promoting wound healing, softening blood vessels, and promoting diuresis. Kudzu root is widely used to treat various diseases, such as ethanol poisoning, fatty liver, cardiovascular and cerebrovascular protection, kidney protection, nervous system diseases, diabetes, and menopausal syndrome.

[0003] Among various microorganisms, lactic acid bacteria (LAB) are the most representative probiotics. Their wide distribution and rapid reproduction rate allow them to play important physiological functions in humans and animals. As a generally recognized as safe (GRAS) microorganism, their status is widely accepted. Numerous examples of the application of lactic acid bacteria in the production of foods such as kimchi, cheese, and yogurt exist. Their importance in the field of bioenergy development is also undeniable.

[0004] Alpha-amylase inhibitors can inhibit the activity of salivary and pancreatic amylase in the gastrointestinal tract, hindering or delaying the hydrolysis and digestion of the main carbohydrates in food, reducing the breakdown and absorption of starchy sugars in food, thereby lowering blood sugar, inhibiting the rise in blood sugar concentration, reducing insulin secretion, reducing fat synthesis, and reducing weight, which is beneficial for the dietary treatment of patients with diabetes and obesity.

[0005] Pancreatic lipase inhibitors can reduce weight and prevent obesity by inhibiting the activity of pancreatic lipase, thus preventing the hydrolysis and absorption of dietary fats (triglycerides, TG). Because these inhibitors do not enter the bloodstream, do not act on the nervous system, and do not suppress appetite, they have attracted considerable attention and become a hot research topic.

[0006] Cholesterol esterase inhibitors can effectively reduce cholesterol intake and the occurrence of related diseases by inhibiting the activity of cholesterol esterase. Therefore, inhibiting cholesterol esterase activity is also a method to control obesity and treat hyperlipidemia. Summary of the Invention

[0007] The purpose of this invention is to provide a food-medicine homologous composition, fermentation broth, preparation method, and application of the same as those for assisting in lowering blood sugar and reducing lipids. This invention uses Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root as food-medicine homologous ingredients. These ingredients are first extracted separately with water. The extracts are then mixed in a specific ratio to form a food-medicine homologous composition, which has good blood sugar and lipid-reducing functions. The food-medicine homologous composition is then fermented with a mixture of four strains: Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10, resulting in a fermentation broth that exhibits even more superior blood sugar and lipid-reducing functions.

[0008] The technical solution for achieving the objective of this invention is as follows:

[0009] This medicinal and edible composition, which has the function of assisting in lowering blood sugar and reducing fat, is made from Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root in a mass ratio of 2~4:1:1:2~4:2~4:2~4.

[0010] Preferably, the mass ratio of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root is 3:1:1:3:3:3.

[0011] The preparation method of the above-mentioned food-medicine homologous composition with auxiliary blood sugar lowering and lipid reduction functions includes the following steps:

[0012] (1) Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root, six kinds of medicinal and edible ingredients were mixed with distilled water at a mass ratio of 1:15 and then treated in a water bath. After the water bath, the mixture was cooled to room temperature, centrifuged and the supernatant was collected to obtain the water extract of each medicinal and edible ingredient.

[0013] (2) Mix the aqueous extracts of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root in a mass ratio of 2~4:1:1:2~4:2~4:2~4 and sterilize to obtain a food-medicine homology composition.

[0014] The fermentation broth of the food-medicine homology composition with auxiliary blood sugar and lipid reduction function is prepared by fermenting the above-mentioned food-medicine homology composition with mixed bacteria formed by mixing Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10 in a ratio of 1:1~2:1:1~2.

[0015] The preparation method of the above-mentioned fermentation broth of the food-medicine homology composition with auxiliary blood sugar lowering and lipid reduction functions includes the following steps:

[0016] (1) Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root, six kinds of medicinal and edible ingredients were mixed with distilled water at a mass ratio of 1:15 and then treated in a water bath. After the water bath, the mixture was cooled to room temperature, centrifuged and the supernatant was collected to obtain the water extract of each medicinal and edible ingredient.

[0017] (2) Mix the aqueous extracts of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root in a mass ratio of 2~4:1:1:2~4:2~4:2~4 and sterilize to obtain a food-medicine homology composition;

[0018] (3) The activated Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10 were mixed in a ratio of 1:1~2:1:1~2 and then inoculated into the food-medicine homology composition. The inoculation amount was 2%~4%, and fermentation was carried out at 37℃ for 6~18h to obtain the fermentation broth of the food-medicine homology composition.

[0019] Preferably, in step (1), the water bath temperature is 85±5℃ and the water bath time is 2~3h.

[0020] Preferably, in step (1), the centrifugation speed is 6500~7000 r / min and the centrifugation time is 30~40 min.

[0021] Preferably, in step (2), the sterilization method is high-temperature sterilization at 121°C for 15 minutes.

[0022] Preferably, in step (3), the inoculum amount is 4% and the fermentation time is 18h.

[0023] A food-medicine composition preparation with auxiliary functions of lowering blood sugar and reducing lipids contains the above-mentioned food-medicine composition or fermentation broth of food-medicine composition.

[0024] The application of the above-mentioned food-medicine homology composition or food-medicine homology composition fermentation broth in the preparation of products with hypoglycemic and lipid-reducing functions.

[0025] Furthermore, the aforementioned products include, but are not limited to, food or medicine.

[0026] Compared with the prior art, the present invention has the following advantages:

[0027] (1) The present invention uses Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root as raw materials, and by adjusting the proportion of each food and medicine homology ingredients, a food and medicine homology composition with good auxiliary hypoglycemic and lipid reduction function is prepared. Its inhibition rate on α-amylase is 64.1%, the inhibition rate on pancreatic lipase is 36.6%, and the inhibition rate on cholesterol esterase is 40.8%.

[0028] (2) Based on the food-medicine homology composition with good auxiliary hypoglycemic and lipid reduction functions, the present invention screens lactic acid bacteria and uses the preferred strains for mixed fermentation. The fermentation broth of the food-medicine homology composition formed exhibits even better auxiliary hypoglycemic and lipid reduction functions. Its inhibition rate on α-amylase is 55.7%, the inhibition rate on pancreatic lipase is increased to 62.9%, and the inhibition rate on cholesterol esterase is increased to 66.5%. The comprehensive inhibition ability of the three enzymes is significantly improved.

[0029] (3) The raw materials used in this invention are food and medicine homologous ingredients, which are safe. Food and medicine homologous compositions and fermentation liquids of food and medicine homologous compositions have broad application prospects in the field of functional foods or drugs that help lower blood sugar and reduce fat. Attached Figure Description

[0030] Figure 1 The inhibitory capacity of three enzymes in the aqueous extracts of six food and medicine ingredients.

[0031] Figure 2 The inhibitory capacity of three enzymes in the food-medicine homology composition after fermentation by different strains.

[0032] Figure 3 The effect of the mixing ratio of the food-medicine homology composition on the inhibitory capacity of three enzymes in the fermentation broth of the food-medicine homology composition.

[0033] Figure 4 The effect of the mixing ratio of strains on the inhibitory capacity of three enzymes in the fermentation broth of the food-medicine homology composition.

[0034] Figure 5 The effect of inoculum quantity on the inhibitory capacity of three enzymes in the fermentation broth of a food-medicine homology composition.

[0035] Figure 6 The effect of fermentation time on the inhibitory capacity of three enzymes in the fermentation broth of the food-medicine homology composition was investigated.

[0036] Figure 7 The effect of different conditions on the inhibitory capacity of the three enzymes in an orthogonal experiment. Detailed Implementation

[0037] The technical solution of the present invention will be clearly and completely described below with reference to specific embodiments and accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] The *Lactobacillus rhamnosus* Hsryfm1301 used in this invention, with accession number CGMCC NO.8545, has been fully disclosed in Chinese patent ZL 201310751034.X; *Lactobacillus rhamnosus* grx603, with accession number CGMCC No.22693, has been fully disclosed in Chinese patent ZL 202111220369.X; *Lactococcus lactis* grx602, with accession number CGMCC No.22692, has been fully disclosed in Chinese patent ZL 202110960058.0; and *Lactobacillus rhamnosus* grx10, with accession number CGMCC NO.2526, has been fully disclosed in Chinese patent ZL 200810023014.X.

[0039] In the following examples, the culture medium used was prepared by the following method:

[0040] MRS liquid culture medium: Weigh 54.00 g of MRS broth and add it to 1.00 L of pure water, then sterilize at 121°C for 15 min.

[0041] MRS solid medium: Add 15.00 g agar powder to every 1.00 L of MRS liquid medium and sterilize at 121℃ for 15 min.

[0042] In the following examples, the methods for determining the inhibitory activity of three enzymes—α-amylase, pancreatic lipase, and cholesterol esterase—are as follows:

[0043] (1) Determination of α-amylase inhibitory capacity:

[0044] Test reagents: PBS buffer (0.01M, pH 6.8), α-amylase solution (3U / mL), soluble starch solution (0.5%), DNS reagent.

[0045] Mix 50 μL of sample solution with 50 μL of α-amylase solution and incubate at 37℃ for 10 min. After incubation, add 50 μL of substrate starch solution and mix well. Incubate again at 37℃ for 10 min. Then, inactivate the enzyme in a 90℃ water bath for 1 min. Add 50 μL of DNS reagent solution to the above solution and continue incubating in a 90℃ water bath for 10 min. Finally, dilute the mixture with 900 μL of distilled water. Take 200 μL of the diluted solution and measure its absorbance at 540 nm. Each experiment was performed in triplicate. The formula for calculating the α-amylase inhibitory capacity is as follows:

[0046] α-Amylase inhibitory capacity = [1 - (A1 - A2) / (A3 - A4)] × 100%,

[0047] In the formula, A1 is the absorbance of the sample after reaction (sample + enzyme + starch), A2 is the blank absorbance (sample + buffer + starch), A3 is the test absorbance (water + enzyme + soluble starch), and A4 is the model absorbance (water + buffer + soluble starch).

[0048] (2) Determination of pancreatic lipase inhibition capacity:

[0049] Test reagents: Tris-HCl buffer (10 mmol / L, pH 7.93), pancreatic lipase solution (5 U / mL), PNPB (2,4-dinitrophenol butyrate solution, 2 mmol / L).

[0050] Add 50 μL of sample solution and an equal volume of pancreatic lipase solution to a 96-well plate, incubate at 37°C for 10 min, then add 50 μL of PNPB solution and react at 37°C for 20 min. Measure the absorbance at 405 nm. Each experiment was performed in triplicate. The formula for calculating the pancreatic lipase inhibitory capacity is as follows:

[0051] Pancreatic lipase inhibition capacity = [1 - (A1 - A2) / (A3 - A4)] × 100%

[0052] In the formula, A1 is the absorbance of the sample after reaction (sample + enzyme + PNPB); A2 is the blank absorbance (sample + buffer + PNPB); A3 is the test absorbance (water + enzyme + PNPB); and A4 is the model absorbance (water + buffer + PNPB).

[0053] (3) Determination of cholesterol esterase inhibition capacity:

[0054] Test reagents: Tris-HCl buffer (10 mmol / L, pH 7.93), cholesterol esterase solution (0.163 U / mL), PNPB (2,4-dinitrophenol butyrate solution, 2 mmol / L).

[0055] Add 50 μL of sample solution and an equal volume of PNPB solution to a 96-well plate, incubate at 37°C for 10 min, then add 50 μL of cholesterol esterase solution, react at 37°C for 20 min, and measure the absorbance at 405 nm. Each experiment was performed in triplicate. The formula for calculating the cholesterol esterase inhibitory capacity is as follows:

[0056] Cholesterol esterase inhibitory capacity = [1 - (A1 - A2) / (A3 - A4)] × 100%

[0057] In the formula, A1 is the absorbance of the sample after reaction (sample + PNPB + enzyme), A2 is the blank absorbance (sample + PNPB + buffer), A3 is the test absorbance (water + PNPB + enzyme), and A4 is the model absorbance (water + PNPB + buffer).

[0058] Example 1

[0059] 1. Preparation of food-medicine homology compositions

[0060] (1) Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root were mixed with distilled water at a mass ratio of 1:15 and placed in a water bath at 85°C for 2 hours. After the water bath, the mixture was taken out and cooled to room temperature. After centrifugation at 6500 r / min for 30 minutes, the supernatant was collected to obtain the water extract of each food and medicine homology ingredient.

[0061] The inhibitory activity of three enzymes in the aqueous extracts of various food and medicinal ingredients was determined, and the results are as follows: Figure 1 As shown. By Figure 1It was found that the inhibition rates of α-amylase varied significantly among different extracts (p<0.05). Hawthorn and Codonopsis extracts showed the highest inhibition rates, at 68.6% and 64.2% respectively, significantly higher than those of kudzu root (53.3%), white kidney bean (33.9%), lotus leaf (27.9%), and malt (23.4%) (p<0.05). Kudzu root was significantly higher than white kidney bean, lotus leaf, and malt (p<0.05). The inhibitory activities of different extracts on pancreatic lipase also varied significantly (p<0.05), with white kidney bean showing the highest inhibition rate at 46.4%, significantly better than the other groups (p<0.05). The inhibitory rates of buds, codonopsis, and kudzu root were significantly higher than those of lotus leaf (18.7%) and hawthorn (13.5%), with inhibition rates of 29.8%, 28.7%, and 26.2%, respectively (p<0.05). All extracts also showed significant inhibitory activity against cholesterol esterase (p<0.05). Hawthorn and white kidney bean showed the highest inhibition rates (37.3% and 34.5%, respectively), significantly higher than the other samples (p<0.05). Kudzu root, malt, and codonopsis root were in the middle range, with inhibition rates of 28.2%, 26.9%, and 23.1%, respectively, significantly better than lotus leaf (14.8%) (p<0.05). The arithmetic mean of the inhibition rates of α-amylase, pancreatic lipase, and cholesterol esterase was used to characterize the overall inhibitory capacity and to assess the overall intervention level of the samples on the digestive enzyme system. The overall inhibitory capacity of the samples differed significantly (p<0.05). Hawthorn (39.8%), Codonopsis (38.7%), and white kidney bean (38.3%) showed the highest overall inhibition rates, with no statistically significant differences among the three (p>0.05), but all were significantly higher than malt (26.7%) and lotus leaf (20.5%) (p<0.05). Kudzu root had an overall inhibition rate of 35.9%, falling between white kidney bean and malt, indicating a moderately high overall enzyme-lowering activity. Hawthorn and white kidney bean showed relatively balanced and efficient inhibitory potential among the three target enzymes, while lotus leaf exhibited the lowest overall activity.

[0062] (2) Mix the water extracts of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root in equal mass ratio, and sterilize them at 121°C for 15 minutes in an autoclave to obtain a food-medicine homology composition. Store it in a refrigerator at 4°C for later use.

[0063] 2. Inoculation and fermentation

[0064] (1) 300 μL of seed culture of 21 lactic acid bacteria strains (strain 1132, strain 1133, strain 1157-2, strain 1167, strain 1174, strain 1474, strain 1477, strain 1517, strain 1564, strain 1565, strain 1574, strain 1587, strain 1590, strain 1600, strain 1601, strain 1609, strain 1613, strain 1617, strain 1626, strain 1630, strain 1676) were inoculated into 10 mL of MRS broth medium, mixed well, and cultured at 37℃ for 16-20 h to obtain activated bacterial culture. Among them, strain 1630 is Lactobacillus rhamnosus Hsryfm1301, strain 1600 is Lactobacillus rhamnosus grx603, strain 1617 is Lactococcus lactis grx602, and strain 1565 is Lactobacillus rhamnosus grx10.

[0065] (2) The activated strains were inoculated into the food-medicine homology composition and fermented. The inoculation amount was 3%, the fermentation temperature was 37℃, and the fermentation time was 24h.

[0066] The inhibitory activity of three enzymes—α-amylase, pancreatic lipase, and cholesterol esterase—was measured in various fermentation broths before fermentation and 24 hours after fermentation. The results are as follows: Figure 2 As shown.

[0067] Depend on Figure 2 It was found that after fermentation by 21 different strains, the inhibitory activities of α-amylase, pancreatic lipase, and cholesterol esterase in the food-medicine homology composition showed significant differences. Among them, the four strains with the best overall inhibitory activity were strain 1630, strain 1600, strain 1617, and strain 1565. The sample fermented by strain 1630 showed the highest inhibition capacity for pancreatic lipase (39.7%), α-amylase (41.5%), and cholesterol esterase (43.3%). The sample fermented by strain 1600 showed the highest inhibition capacity for cholesterol esterase (54.7%), α-amylase (27.9%), and cholesterol esterase (37.8%). The sample fermented by strain 1617 exhibited the strongest inhibition capacity for α-amylase (44.4%), pancreatic lipase (38.9%), and cholesterol esterase (28.8%). The sample fermented by strain 1565 showed the strongest inhibition capacity for α-amylase (25.0%), pancreatic lipase (35.2%), and cholesterol esterase (51.3%). Therefore, this invention selects strains 1630 (Lactobacillus rhamnosus Hsryfm1301), 1600 (Lactobacillus rhamnosus grx603), 1617 (Lactococcus lactis grx602), and 1565 (Lactobacillus rhamnosus grx10) as fermentation strains to ferment the food-medicine homology composition.

[0068] Example 2

[0069] The comprehensive inhibitory effect of the fermentation broth of the food-medicine homology composition on three enzymes—α-amylase, pancreatic lipase, and cholesterol esterase—was used as the evaluation index. The method of controlling variables was adopted to investigate the mixing ratio of water extracts of each food-medicine homology ingredient, the mixing ratio of the four strains, the inoculum amount, and the fermentation time.

[0070] 1. The effect of the mixing ratio of water extracts from various food and medicinal ingredients:

[0071] The ratio of *Lactobacillus rhamnosus* Hsryfm1301, *Lactobacillus rhamnosus* grx603, *Lactococcus lactis* grx602, and *Lactobacillus rhamnosus* grx10 was fixed at 1:2:1:2, the inoculum amount was 3%, and the fermentation time was 24 h. The effect of the mixing ratio of the aqueous extracts of each medicinal and edible ingredient on the comprehensive inhibitory ability of the three enzymes in the fermentation broth of the medicinal and edible ingredient combination was investigated. The mass ratios of the aqueous extracts of *Codonopsis pilosula*, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root were set as 2:1:1:2:2:2, 3:1:1:3:3:3, 4:1:1:4:4:4, 5:1:1:5:5:5, and 6:1:1:6:6:6.

[0072] like Figure 3 As shown, when the ratio of Codonopsis pilosula aqueous extract, malt aqueous extract, lotus leaf aqueous extract, white kidney bean aqueous extract, hawthorn aqueous extract and kudzu root aqueous extract is 3:1:1:3:3:3, the fermentation broth of the food-medicine homology composition has the highest comprehensive inhibitory capacity for the three enzyme activities, with α-amylase inhibition capacity of 44.9%, pancreatic lipase inhibition capacity of 48.0%, and cholesterol esterase inhibition capacity of 55.9%.

[0073] 2. The effect of the mixing ratio of the four strains:

[0074] The mass ratio of aqueous extracts of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root was fixed at 4:1:1:4:4:4. The fermentation time was 24 hours, and the inoculum amount was 3%. The effect of different mixing ratios of the four strains on the comprehensive inhibitory ability of the fermentation broth of the food-medicine homology composition on the activities of three enzymes was investigated. The ratios of Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10 were set as 1:1:1:1, 1:1.5:1:1.5, 1:2:1:2, 1:2.5:1:2.5, and 1:3:1:3, respectively.

[0075] like Figure 4As shown, when the ratio of Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10 was 1:1.5:1:1.5, the fermentation broth of the food-medicine homology composition exhibited the highest comprehensive inhibitory capacity for the three enzyme activities, with α-amylase inhibition capacity of 58.4%, pancreatic lipase inhibition capacity of 51.4%, and cholesterol esterase inhibition capacity of 53.1%.

[0076] 3. The effect of inoculum quantity:

[0077] The mass ratio of aqueous extracts of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root was fixed at 4:1:1:4:4:4. The ratio of Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10 was 1:2:1:2. The fermentation time was 24 h. The effect of different inoculum amounts on the comprehensive inhibitory ability of the three enzyme activities in the fermentation broth of the food-medicine homology composition was investigated. The inoculum amounts were set at 1%, 2%, 3%, 4%, and 5%.

[0078] like Figure 5 As shown, when the inoculum content is 2%, the fermentation broth of the food-medicine homology composition exhibits the highest comprehensive inhibitory capacity for the three enzyme activities, with α-amylase inhibition capacity at 56.3%, pancreatic lipase inhibition capacity at 48.4%, and cholesterol esterase inhibition capacity at 46.6%.

[0079] 4. The effect of fermentation time

[0080] The mass ratio of aqueous extracts of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root was fixed at 4:1:1:4:4:4. The ratio of Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10 was 1:2:1:2. The inoculum amount was 3%. The effect of different fermentation times on the comprehensive inhibitory ability of the three enzymes in the fermentation broth of the food-medicine homology composition was investigated. The fermentation times were set at 6h, 12h, 24h, 48h, and 96h.

[0081] like Figure 6 As shown, when the fermentation time is 12 hours, the fermentation broth of the food-medicine homology composition has the highest comprehensive inhibitory capacity for the three enzyme activities, with α-amylase inhibition capacity of 67.5%, pancreatic lipase inhibition capacity of 56.1%, and cholesterol esterase inhibition capacity of 55.9%.

[0082] In summary, the following mixing ratios were selected for subsequent orthogonal experiments: 2:1:1:2:2:2, 3:1:1:3:3:3, and 4:1:1:4:4:4 for the aqueous extracts of various food and medicinal ingredients; 1:1:1:1, 1:1.5:1:1.5, and 1:2:1:2 for the four strains; 2%, 3%, and 4% for the inoculum amount; and 6, 12, and 18 hours for the fermentation time.

[0083] Example 3

[0084] Based on the single-factor experimental results of Example 2, an orthogonal experiment was designed with the mixing ratio of water extracts of various food and medicinal ingredients, the mixing ratio of the four strains, the inoculum amount, and the fermentation time as experimental factors. The factor levels are shown in Table 1 below.

[0085] Table 1 Formulation Factor Level Table

[0086]

[0087] According to L9(3) 4 Experiments were conducted under the process conditions required in the table, with three parallel groups designed. The comprehensive inhibitory capacity of the three enzymes, α-amylase, pancreatic lipase, and cholesterol esterase, was used as the evaluation index to verify the predicted results.

[0088] Table 2. Results of Orthogonal Experiments

[0089]

[0090] From Table 2 and Figure 7 It can be seen that, through range analysis of the orthogonal experimental results of the fermentation broth of the food-medicine homology composition, the order of influence on the comprehensive inhibitory ability of the three enzymes—α-amylase, pancreatic lipase, and cholesterol esterase—was A>B>C>D. Therefore, A2B3C3D3 was selected as the optimal preparation conditions. Measurements verified that under these optimal conditions, the food-medicine homology composition already possessed good auxiliary functions for lowering blood sugar and reducing lipids before fermentation. Its inhibition rate against α-amylase was 64.1%, against pancreatic lipase was 36.6%, and against cholesterol esterase was 40.8%. The inhibition capacity of the fermentation broth against α-amylase was 55.7%, against pancreatic lipase was 62.9%, against cholesterol esterase was 66.5%, and the comprehensive inhibition capacity was 61.6%. Therefore, the optimal preparation conditions for the fermentation broth of the food-medicine homology composition are: a mixing ratio of 3:1:1:3:3:3 for the aqueous extracts of each food-medicine homology ingredient, a mixed bacterial ratio of 1:2:1:2, an inoculum amount of 4%, and a fermentation time of 18 hours.

Claims

1. A food-medicine composition with auxiliary functions of lowering blood sugar and reducing lipids, characterized in that, It is made from Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root in a mass ratio of 2~4:1:1:2~4:2~4:2~4.

2. The medicinal and edible composition according to claim 1, characterized in that, The mass ratio of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn, and kudzu root is 3:1:1:3:3:

3.

3. The method for preparing the food-medicine homology composition according to claim 1 or 2, characterized in that, Includes the following steps: (1) Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root, six kinds of medicinal and edible ingredients were mixed with distilled water at a mass ratio of 1:15 and then treated in a water bath. After the water bath, the mixture was cooled to room temperature, centrifuged and the supernatant was collected to obtain the water extract of each medicinal and edible ingredient. (2) Mix the aqueous extracts of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root in a mass ratio of 2~4:1:1:2~4:2~4:2~4 and sterilize to obtain a food-medicine homology composition.

4. A fermentation broth of a food-medicine homologous composition with auxiliary blood sugar lowering and lipid reduction functions, characterized in that, The food-medicine composition of claim 1 or 2 is prepared by fermentation of a mixed bacteria consisting of Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10 in a ratio of 1:1~2:1:1~2.

5. The method for preparing the fermentation broth of the food-medicine homology composition according to claim 4, characterized in that, Includes the following steps: (1) Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root, six kinds of medicinal and edible ingredients were mixed with distilled water at a mass ratio of 1:15 and then treated in a water bath. After the water bath, the mixture was cooled to room temperature, centrifuged and the supernatant was collected to obtain the water extract of each medicinal and edible ingredient. (2) Mix the aqueous extracts of Codonopsis pilosula, malt, lotus leaf, white kidney bean, hawthorn and kudzu root in a mass ratio of 2~4:1:1:2~4:2~4:2~4 and sterilize to obtain a food-medicine homology composition; (3) The activated Lactobacillus rhamnosus Hsryfm1301, Lactobacillus rhamnosus grx603, Lactococcus lactis grx602, and Lactobacillus rhamnosus grx10 were mixed in a ratio of 1:1~2:1:1~2 and then inoculated into the food-medicine homology composition. The inoculation amount was 2%~4%, and fermentation was carried out at 37℃ for 6~18h to obtain the fermentation broth of the food-medicine homology composition.

6. The preparation method according to claim 3 or 5, characterized in that, In step (1), the water bath temperature is 85±5℃ and the water bath time is 2~3h; the centrifugation speed is 6500~7000 r / min and the centrifugation time is 30~40min; in step (2), the sterilization method is high temperature sterilization at 121℃ for 15min.

7. The preparation method according to claim 5, characterized in that, In step (3), the inoculum amount is 4% and the fermentation time is 18h.

8. A medicinal and edible homologous composition preparation with auxiliary functions of lowering blood sugar and reducing lipids, characterized in that, The fermentation broth contains the food-medicine homology composition according to claim 1 or 2, or the food-medicine homology composition according to claim 4.

9. The application of the food-medicine homology composition according to claim 1 or 2, or the fermentation broth of the food-medicine homology composition according to claim 4, in the preparation of products with auxiliary hypoglycemic and lipid-reducing functions.

10. The application according to claim 9, characterized in that, The product is either food or medicine.

Images