A method, apparatus and system for blood analysis

Abstract

This invention relates to the field of blood analysis technology, and discloses a blood analysis method, apparatus, and system. The blood analysis method includes the following steps: acquiring optical modulation information, which refers to the information of light modulated by magnetic beads that undergo Brownian rotation driven by an oscillating magnetic field in the blood sample; processing the optical modulation information to obtain the analysis results of the blood sample, including magnetic bead-based coagulation detection results and optical coagulation detection results of the blood sample. Applying the technical solution of this invention, it is possible to simultaneously obtain both optical and magnetic bead-based detection results from a single detection area, a single blood sample, and a single detection operation. It enables real-time dynamic detection of the magnetic bead coagulation curve and the viscosity of the blood sample, and features higher detection and analysis efficiency, lower cost, higher accuracy, and more comprehensive results.

Description

Technical Field

[0001] This invention relates to the field of blood analysis technology, specifically to a blood analysis method, apparatus, and system. Background Technology

[0002] Blood coagulation analysis is a key method for diagnosing hemorrhagic and thrombotic diseases, assessing surgical risks, and monitoring anticoagulation therapy. Existing coagulation analysis methods are mainly divided into two categories: optical methods and magnetic bead methods. Optical methods are based on changes in turbidity during the coagulation process, analyzing the intensity of transmitted / scattered light. They are characterized by high sensitivity, low cost, and ease of automation, but are susceptible to optical interference signals such as lipemia, jaundice, hemolysis, bubbles, and the smoothness of the test cup. Magnetic bead methods are based on the restriction of magnetic bead movement during the coagulation process, analyzing the amplitude of magnetic bead translation through an induction coil. This effectively avoids optical interference, but has the following drawbacks: (1) It is difficult to provide information on the continuous changes in viscosity and coagulation state over time, therefore it can only reflect the coagulation endpoint time and cannot output a coagulation curve, making it difficult to identify interference; (2) Magnetic beads move along the cup wall or bottom, easily affected by the smoothness of the inner wall of the test cup; (3) Magnetic beads have millimeter or sub-millimeter diameters and large mass, and translation may damage the fibrin network, causing result bias.

[0003] Since optical and magnetic bead methods each have their own advantages and disadvantages, both methods are often used simultaneously in practice. Therefore, medical institutions or clinical laboratories and other testing and analysis organizations typically need to purchase both optical and magnetic bead products, significantly increasing equipment costs, occupying more testing space, and reducing testing efficiency. To address these issues, some existing technologies attempt to achieve simultaneous testing of both methods through structural integration. For example, in patent application CN107315094A, optical and magnetic bead detection structures are installed on the same device, automatically selecting the appropriate detection module to perform the test by identifying the sample type. Another example is patent application CN119125587A, which further integrates the two detection structures, enabling separate optical and magnetic bead testing in different areas of the testing cup.

[0004] However, the existing integrated solutions of optical and magnetic bead methods essentially achieve the simultaneous deployment of the two methodologies by spatially superimposing structures, which does not truly achieve "simultaneous detection of the same source sample". They still suffer from the shortcomings of complex detection structures, high equipment costs and failure rates. Furthermore, their time-sharing or zone-sharing detection modes limit detection efficiency and the depth of information fusion, making it difficult to meet the clinical demand for efficient, accurate and low-cost detection. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the purpose of this application is to provide a blood analysis method, apparatus and system that aims to alleviate or eliminate at least one of the above-mentioned technical problems.

[0006] In a first aspect, embodiments of this application provide a blood analysis method, comprising the following steps:

[0007] Acquire optical modulation information, which refers to the information of light modulated by magnetic beads in a blood sample that are driven by an oscillating magnetic field to perform Brownian rotation;

[0008] The optical modulation information is processed to obtain the analysis results of the blood sample, which include magnetic bead coagulation detection results and optical coagulation detection results.

[0009] In some embodiments, processing the optical modulation information to obtain the analysis results of the blood sample includes the following steps: extracting harmonic signals from the optical modulation information that are frequency-harmonics related to the driving frequency of the oscillating magnetic field, and obtaining the amplitude of the harmonic signals over time.

[0010] In some embodiments, processing the optical modulation information to obtain the analysis results of the blood sample includes the following steps: synchronously integrating and averaging the total optical density determined based on the optical modulation information over one or more magnetic excitation cycles, and subtracting the steady-state optical density of the magnetic bead to obtain the relationship between the optical density of the blood sample and time.

[0011] In some embodiments, the analysis results also include viscosity measurement results.

[0012] In some embodiments, processing the optical modulation information to obtain the analysis results of the blood sample includes the following steps: extracting harmonic signals that are frequency-harmonic with the driving frequency of the oscillating magnetic field from the optical modulation information, obtaining the relationship between the amplitude of the harmonic signals and the driving frequency of the oscillating magnetic field, identifying the peak frequency of the relationship, and calculating the absolute viscosity of the blood sample based on the peak frequency.

[0013] In some embodiments, processing the optical modulation information to obtain the analysis results of the blood sample includes the following steps:

[0014] For the time-domain voltage signal formed based on the optical modulation information Normalization is performed to obtain the normalized time-domain signal. , The mean of the reference signal when there is no magnetic field is used. A Hanning window is applied to the normalized time-domain signal and a fast Fourier transform is performed to obtain the spectrum. The real part of the second harmonic signal, which is twice the driving frequency of the oscillating magnetic field, is extracted from the spectrum. The real part of the second harmonic signal satisfies Among them, amplitude factor satisfy ,

[0015] The Brownian relaxation time of the magnetic bead. Let be the concentration of the magnetic beads, and z be the optical path length of the light ray. For the magnetic moment of a single magnetic bead, Boltzmann's constant, For absolute temperature; continuously calculate the amplitude factor at different time points. With detection time as the x-axis and amplitude factor as the y-axis, The magnetic bead coagulation curve is obtained by using the vertical axis, and the magnetic bead coagulation time is determined based on the magnetic bead coagulation curve.

[0016] The total optical density determined based on the optical modulation information Synchronous integral averaging is performed over one or more magnetic excitation cycles T to obtain... ;according to Calculate the optical density of the blood sample. The steady-state optical density of the magnetic beads; the optical density of the blood sample with detection time as the x-axis. The optical solidification curve is obtained by using the ordinate.

[0017] Obtain the real part of the second harmonic signal With the driving frequency of the oscillating magnetic field The relationship between the changes is analyzed, and the peak frequency corresponding to the characteristic peak in the relationship is identified. According to the formula Calculate the absolute viscosity of the blood sample at the current moment. ,in The hydrodynamic volume of the magnetic bead; the absolute viscosity at different time points is calculated continuously. .

[0018] In some embodiments, the following steps are also included:

[0019] Place the blood sample and a predetermined number of magnetic beads in the container;

[0020] An oscillating magnetic field is applied to the container to drive the magnetic bead to perform Brownian rotation;

[0021] The container is illuminated with light, which passes through the area where the magnetic bead is located. The light modulated by the magnetic bead is collected to obtain light modulation information.

[0022] In some embodiments, the magnetic beads have a particle size of 50-5000 nm, and the concentration of the magnetic beads in the blood sample is [missing information]. The driving frequency of the oscillating magnetic field is 0.1-1000 Hz, and the intensity is 0.1-5 mT.

[0023] Secondly, embodiments of this application provide an apparatus including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the computer program to implement the blood analysis method described above.

[0024] Thirdly, embodiments of this application provide a system including a magnetic field generator, a light source, a detection unit, and the aforementioned apparatus.

[0025] The magnetic field generator is used to apply an oscillating magnetic field to a container containing a blood sample and a preset number of magnetic beads, so as to drive the magnetic beads to perform Brownian rotation.

[0026] The light source is used to emit light to illuminate the container, and the light passes through the area where the magnetic bead is located;

[0027] The detection unit is used to collect light that has been modulated by the magnetic beads in order to obtain light modulation information;

[0028] The device is used to process the optical modulation information.

[0029] Compared with the prior art, the present invention has the following significant advantages:

[0030] Higher detection and analysis efficiency and lower detection and analysis cost: A single detection and analysis can simultaneously obtain multiple information such as optical method results, magnetic bead method results, real-time viscosity data and magnetic solidification curves, without the need for separate detection or the purchase of multiple additional devices, which greatly reduces the equipment investment cost of medical institutions and improves detection efficiency.

[0031] Simpler structure and lower failure rate: Dual-method detection can be achieved without the need for spatial structure integration. Simultaneous dual-method detection can be completed with only one detection area and one sample, which simplifies the equipment structure and reduces the equipment failure rate and maintenance costs.

[0032] Higher detection accuracy and stronger anti-interference ability: Based on Brownian rotation of nanoscale magnetic beads, the magnetic beads are in a uniformly dispersed suspension in the sample, which reduces frictional interference from the detection container and damage to the fibrin network, thus improving the reliability of the detection results.

[0033] More comprehensive detection information: It can obtain coagulation curves and real-time viscosity data using the magnetic bead method, providing a more comprehensive basis for interference analysis and clinical coagulation function assessment. Attached Figure Description

[0034] To more clearly illustrate the technical solutions in the embodiments of this application or the background art, the accompanying drawings used in the embodiments of this application will be described below.

[0035] Figure 1 This is a flowchart of the blood analysis method disclosed in the embodiments of this application;

[0036] Figure 2 This is a schematic diagram of a system disclosed in one embodiment of this application;

[0037] Figure 3 This is a schematic diagram of a system disclosed in another embodiment of this application.

[0038] Explanation of reference numerals in the attached figures:

[0039] 1-Light source; 2-Magnetic field generator; 3-Container; 4-Detection unit; 5-Light beam; 6-Reflector. Detailed Implementation

[0040] The terms “first,” “second,” etc., are used for descriptive purposes only and have no sequential or technical meaning, nor should they be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated.

[0041] The embodiments of this application are described below with reference to the accompanying drawings.

[0042] like Figure 1 As shown in the figure, this application provides a blood analysis method, including the following steps:

[0043] S100: Acquire optical modulation information, which refers to the information of light modulated by magnetic beads in the blood sample that are driven by an oscillating magnetic field to perform Brownian rotation;

[0044] S200: Processes optical modulation information to obtain analysis results of blood samples, including magnetic bead coagulation test results and optical coagulation test results.

[0045] When light passes through a magnetic bead undergoing Brownian rotation driven by an external magnetic field, the light modulation information formed by the blocking / reflection by the bead has a frequency harmonic relationship with the magnetic field driving the bead's rotation. (Definition) The difference in extinction cross-sections when the major axis of the magnetic bead is parallel and perpendicular to the optical path during Brownian rotation represents the anisotropy and fixation of the magnetic bead as a whole during the coagulation process, accurately reflecting the degree of coagulation of the blood sample. Since the Brownian rotation of the magnetic bead generates a periodic optical modulation signal, while the coagulation of the blood sample itself generates a slowly varying optical density signal, these two signals have different characteristics in the frequency and time domains. Therefore, two independent detection results can be decoupled from the same optical modulation information. In other words, it is possible to simultaneously obtain both optical and magnetic bead-based detection results for a single detection area, a single blood sample, and a single detection operation.

[0046] In practical implementation, optical modulation information typically refers to the information of the optical signal received by the detection unit after passing through the magnetic bead. This optical signal can be a transmitted light signal or a scattered light signal, preferably a transmitted light signal. The optical modulation information can simultaneously include both optical and magnetic signals; for example, the optical modulation information includes light intensity signals and modulated light harmonic signals (including their components and phase difference with respect to the excitation magnetic field), etc.

[0047] As a preferred example, the results of magnetic bead coagulation testing include magnetic bead coagulation curves and / or magnetic bead coagulation times; the results of optical coagulation testing include optical coagulation curves and / or optical coagulation times.

[0048] In some embodiments, processing optical modulation information to obtain analysis results of blood samples includes the following steps: extracting harmonic signals that are frequency-harmonic with the driving frequency of the oscillating magnetic field from the optical modulation information, and obtaining the amplitude of the harmonic signals as a function of time.

[0049] A Brownian rotation of nano-magnetic beads is driven by an oscillating magnetic field. The periodic rotation of the beads causes periodic changes in their blocking / reflection of light, with the frequency of these changes being a harmonic of the driving frequency of the magnetic field. Harmonic components are extracted from the optical modulation signal using a Fast Fourier Transform (FFT), and their amplitude reflects the rotational degrees of freedom of the magnetic beads. As blood coagulation progresses, a fibrin network gradually forms, restricting the rotation of the magnetic beads and causing the harmonic amplitude to decrease. By continuously monitoring the change in this amplitude over time, the coagulation curve of the magnetic bead method can be obtained, and the coagulation time can be determined from it. This method is unaffected by optical interference from the sample (such as lipemia, jaundice, hemolysis), and because it uses nano-sized magnetic beads, it avoids the mechanical damage to the fibrin network caused by the translational motion of the magnetic beads and the interference from friction of the detection cup wall, which are present in traditional magnetic bead methods. Preferably, the second harmonic signal, which is a harmonic of the driving frequency, is extracted from the optical modulation information.

[0050] In some embodiments, processing optical modulation information to obtain the analysis results of a blood sample includes the following steps: synchronously integrating and averaging the total optical density determined based on the optical modulation information over one or more magnetic excitation cycles, and subtracting the steady-state optical density of the magnetic beads to obtain the relationship between the optical density of the blood sample and time.

[0051] Using the above technical solution, the total optical density signal comprises three components: the optical density of the blood sample itself (a slowly varying aperiodic signal), the periodic component of the magnetic beads (an alternating signal with zero mean), and the steady-state optical density of the magnetic beads (a constant value). By simultaneously integrating and averaging the total optical density over one or more magnetic excitation cycles, the periodic component is eliminated. Then, by subtracting the pre-calibrated steady-state optical density of the magnetic beads, the optical density of the blood sample itself can be decoupled and obtained. Plotting time on the x-axis and the optical density of the blood sample on the y-axis yields the optical coagulation curve, which can be used to extract the optical coagulation time. This scheme enables the acquisition of pure optical detection results unaffected by magnetic beads in blood samples containing magnetic beads, achieving compatibility between photomagnetic and optical methods within the same optical path.

[0052] In some embodiments, the analysis results also include viscosity measurement results.

[0053] The above-mentioned technical solution can not only provide clotting time, but also monitor the absolute viscosity of blood samples in real time, providing more comprehensive rheological parameters for coagulation function assessment.

[0054] In some embodiments, processing optical modulation information to obtain the analysis results of a blood sample includes the following steps: extracting harmonic signals that are frequency-multiplied by the driving frequency of the oscillating magnetic field from the optical modulation information, obtaining the relationship between the amplitude of the harmonic signals and the driving frequency of the oscillating magnetic field, identifying the peak frequency of the relationship, and calculating the absolute viscosity of the blood sample based on the peak frequency.

[0055] Using the above technical solution, by changing the driving frequency of the oscillating magnetic field, the harmonic amplitude at different frequencies is measured, resulting in a spectrum curve showing the amplitude changing with the driving frequency. This spectrum curve exhibits a characteristic peak, the peak frequency of which is related to the Brownian relaxation time of the magnetic bead. According to the formula... The absolute viscosity of the sample at the current moment can be directly calculated. By repeatedly performing frequency sweep measurements on the same coagulation reaction at different time points, the dynamic change of viscosity with the coagulation process can be obtained. This viscosity detection method is based on the rotational dynamics of nanomagnetic beads and does not depend on changes in optical turbidity, thus it is also unaffected by optical interference from the sample. Preferably, the second harmonic signal, which is twice the harmonic of the driving frequency, is extracted from the optical modulation information.

[0056] In some embodiments, processing optical modulation information to obtain analysis results for a blood sample includes the following steps:

[0057] For time-domain voltage signals formed based on optical modulation information Normalization is performed to obtain the normalized time-domain signal. , The mean of the reference signal without a magnetic field is used. A Hanning window is applied to the normalized time-domain signal, and a Fast Fourier Transform is performed to obtain the spectrum. The real part of the second harmonic signal, which is twice the driving frequency of the oscillating magnetic field, is extracted from the spectrum. The real part of the second harmonic signal satisfies Among them, amplitude factor satisfy ,

[0058] The Brownian relaxation time of the magnetic bead. Let be the concentration of the magnetic beads, and z be the optical path length of the light ray. For the magnetic moment of a single magnetic bead, Boltzmann's constant, For absolute temperature; continuously calculate the amplitude factor at different time points. With detection time as the x-axis and amplitude factor as the y-axis, The magnetic bead coagulation curve is obtained by using the vertical axis, and the magnetic bead coagulation time is determined based on the magnetic bead coagulation curve.

[0059] Total optical density determined based on optical modulation information Synchronous integral averaging is performed over one or more magnetic excitation cycles T to obtain... ;according to Calculate the optical density of the blood sample. The steady-state optical density of the magnetic beads is represented by the value of the detection time; the optical density of the blood sample is represented by the value of the detection time on the x-axis. The optical solidification curve is obtained by using the ordinate.

[0060] Obtain the real part of the second harmonic signal With the driving frequency of the oscillating magnetic field The relationship between the changes, and the peak frequencies corresponding to the characteristic peaks in the relationship between the changes. According to the formula Calculate the absolute viscosity of the blood sample at the current moment. ,in The hydrodynamic volume of the magnetic bead; the absolute viscosity at different time points was continuously calculated. .

[0061] By employing the above technical solution, the same optical modulation information acquired in a single detection can be processed through the three parallel or time-division signal processing paths described above to simultaneously or sequentially obtain coagulation time using the magnetic bead method, coagulation curve using the optical method, and real-time dynamic viscosity data. This solution fully leverages the frequency domain characteristics (second harmonic), time-domain slowly varying characteristics (optical density), and frequency sweep characteristics (peak frequency) in the optical modulation information, achieving the technical effect of extracting multidimensional coagulation parameters from a single physical measurement. Moreover, this technical solution provides a specific and feasible analysis process. Based on the above-mentioned normalization processing, fast Fourier transform, second harmonic extraction, synchronous integral averaging, frequency sweep peak identification, and formula calculation steps, it is packaged into a coagulation analysis software library or embedded algorithm module, which can be directly deployed in the control unit of the coagulation detection device to achieve automation and standardization of optical-magnetic fusion coagulation detection.

[0062] As a concrete example, the system's magnetic field generator applies a magnetic field of the form of... The oscillating magnetic field, in which It is the amplitude of the magnetic field. To drive the frequency, the optical modulation signal is captured by the detection unit and converted into a time-domain voltage signal. .

[0063] The principle of optical coagulation detection and analysis is: the optical density of the blood sample itself. It is a slowly varying, aperiodic signal that monotonically increases with the blood coagulation process. Magnetic particle optical density. It includes a periodic alternating component and a steady-state component. The periodic alternating component is generated by the periodic changes in the extinction interface caused by the Brownian rotation of the magnetic beads, while the steady-state component is the constant optical density value after the magnetic beads are fixed by the fibrin mesh. The total optical density signal is analyzed... Perform synchronous integral averaging within the magnetic excitation period. This can eliminate periodic alternating components; Subtract the steady-state optical density of the magnetic particles This allows for the decoupling of the optical density curve of the pure sample as a function of the coagulation process. This optical density curve can be used to extract optical coagulation data. Specific decoupling steps are as follows:

[0064] (1) Extract the periodic components of the magnetic particles, and then... Take the average value over one or more magnetic excitation periods T:

[0065] ,

[0066] (2) Due to the periodic term The mean of the integral over a period is 0, therefore:

[0067] ,

[0068] (3) Final decoupling formula:

[0069] .

[0070] In some embodiments, the following steps are also included:

[0071] Place the blood sample and a predetermined number of magnetic beads in the container;

[0072] An oscillating magnetic field is applied to the container to drive the magnetic bead to perform Brownian rotation;

[0073] The container is illuminated by light, and the light passes through the area where the magnetic beads are located. The light modulated by the magnetic beads is collected to obtain light modulation information.

[0074] The above technical solution can be used to perform the above steps using a system including a magnetic field generator, a light source, and a detection unit, which is easy to implement.

[0075] In some embodiments, the particle size of the magnetic beads is 50-5000 nm, and the concentration of magnetic beads in the blood sample is [missing information]. The driving frequency of the oscillating magnetic field is 0.1-1000 Hz, and the intensity is 0.1-5 mT.

[0076] Within the aforementioned parameter range, the magnetic beads can form a stable, homogeneous suspension in blood samples. Their Brownian rotation is sensitive to magnetic fields without causing mechanical damage to the fibrin network. The optimized range of magnetic field frequency and intensity ensures a high signal-to-noise ratio for the second harmonic signal and clarity of the swept frequency characteristic peaks, resulting in reliable detection results. The surface of the magnetic beads can be further passivated to reduce non-specific adsorption.

[0077] In some embodiments, a variable-frequency oscillating magnetic field can be applied to the container as needed to facilitate the calculation of absolute viscosity.

[0078] In some embodiments, this application provides an apparatus including a memory, a processor, and a computer program stored in the memory and executable on the processor. The processor executes the computer program to implement the blood analysis method described above.

[0079] Using the above technical solution, the device can independently perform data processing functions, receive signals from the detection unit, and output results such as coagulation time, coagulation curve, and viscosity data. This device can be the control motherboard of a dedicated coagulation analyzer, or a functional module formed by loading corresponding software into a general-purpose computer.

[0080] In some embodiments, such as Figure 2 As shown, this application provides a system including a magnetic field generator 2, a light source 1, a detection unit 4, and the aforementioned device.

[0081] The magnetic field generator 2 is used to apply an oscillating magnetic field to the container 3 containing the blood sample and a preset number of magnetic beads to drive the magnetic beads to perform Brownian rotation.

[0082] Light source 1 is used to emit light 5 to illuminate container 3, and light 5 passes through the area where the magnetic bead is located;

[0083] The detection unit 4 is used to collect the light 5 modulated by the magnetic beads to obtain light modulation information;

[0084] The device is used to process optical modulation information.

[0085] Using the above technical solution, the system integrates three major functional modules: magnetic field generation, optical detection, and signal processing, forming a complete coagulation analysis platform. The magnetic field generator 2 can employ a Helmholtz coil or solenoid structure to generate a uniform oscillating magnetic field in the sample area. The light source 1 is preferably in the near-infrared band (e.g., 650 nm–950 nm), which has strong penetration into blood samples and is less affected by heme absorption. The detection unit 4 can employ a silicon photodiode or photomultiplier tube, along with preamplifier and analog-to-digital converter circuits, to convert the optical signal into a digital signal for processing by the device. The container 3 can be a detection cup.

[0086] In practice, the device is connected to the detection unit. The device can receive the light modulation information obtained by the detection unit and process the light modulation information to obtain the analysis results of the blood sample.

[0087] As a specific example, the device can also serve as a control module for a coagulation analysis platform, used to control the operation, parameter settings, and management of the platform.

[0088] In specific implementation, such as Figure 2 and 3 As shown, the relative angle between light ray 5 and magnetic field generator 2 can be adjusted; the angle between light ray 5 and magnetic field generator 2 can be adjusted. to The detection unit 4 can be set on the opposite side of the light source 1, or it can be set in other positions. The light 5 modulated by the magnetic beads in the blood sample that are driven by the oscillating magnetic field to perform Brownian rotation is reflected to the detection unit 4 by the reflector 6.

[0089] This invention proposes to achieve simultaneous detection of both optical and magnetic bead methods in one detection area, one blood sample, and one detection operation, breaking the limitation of traditional methods that require structural integration to deploy both methods on the same machine. Based on the Brownian rotation of nanoscale magnetic beads, this invention can avoid container friction and damage to the fibrin network, and realize real-time dynamic detection of magnetic bead coagulation curve and sample viscosity.

[0090] It should be understood that the application of this application is not limited to the examples above. Those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims. Those skilled in the art can understand that implementing all or part of the processes of the above embodiments and making equivalent changes according to the claims of this application still fall within the scope of this application.

Claims

1. A blood analysis method, characterized in that, Includes the following steps: Acquire optical modulation information, which refers to the information of light modulated by magnetic beads in a blood sample that are driven by an oscillating magnetic field to perform Brownian rotation; The optical modulation information is processed to obtain the analysis results of the blood sample, which include magnetic bead coagulation detection results and optical coagulation detection results.

2. The blood analysis method as described in claim 1, characterized in that, The process of processing the optical modulation information to obtain the analysis results of the blood sample includes the following steps: extracting harmonic signals from the optical modulation information that are frequency-harmonics related to the driving frequency of the oscillating magnetic field, and obtaining the amplitude of the harmonic signals over time.

3. The blood analysis method as described in claim 1, characterized in that, The process of processing the optical modulation information to obtain the analysis results of the blood sample includes the following steps: synchronously integrating and averaging the total optical density determined based on the optical modulation information over one or more magnetic excitation cycles, and subtracting the steady-state optical density of the magnetic bead to obtain the relationship between the optical density of the blood sample and time.

4. The blood analysis method as described in claim 1, characterized in that, The analysis results also include viscosity test results.

5. The blood analysis method as described in claim 4, characterized in that, The process of processing the optical modulation information to obtain the analysis results of the blood sample includes the following steps: extracting harmonic signals that are frequency-harmonic with the driving frequency of the oscillating magnetic field from the optical modulation information, obtaining the relationship between the amplitude of the harmonic signals and the driving frequency of the oscillating magnetic field, identifying the peak frequency of the relationship, and calculating the absolute viscosity of the blood sample based on the peak frequency.

6. The blood analysis method as described in claim 1, characterized in that, The process of processing the optical modulation information to obtain the analysis results of the blood sample includes the following steps: For the time-domain voltage signal formed based on the optical modulation information Normalization is performed to obtain the normalized time-domain signal. , The mean of the reference signal when there is no magnetic field is used. A Hanning window is applied to the normalized time-domain signal and a fast Fourier transform is performed to obtain the spectrum. The real part of the second harmonic signal, which is twice the driving frequency of the oscillating magnetic field, is extracted from the spectrum. The real part of the second harmonic signal satisfies Among them, amplitude factor satisfy , The Brownian relaxation time of the magnetic bead. Let be the concentration of the magnetic beads, and z be the optical path length of the light ray. For the magnetic moment of a single magnetic bead, Boltzmann's constant, For absolute temperature; continuously calculate the amplitude factor at different time points. With detection time as the x-axis and amplitude factor as the y-axis, The magnetic bead coagulation curve is obtained by using the vertical axis, and the magnetic bead coagulation time is determined based on the magnetic bead coagulation curve. The total optical density determined based on the optical modulation information Synchronous integral averaging is performed over one or more magnetic excitation cycles T to obtain... ;according to Calculate the optical density of the blood sample. The steady-state optical density of the magnetic beads; the optical density of the blood sample with detection time as the x-axis. The optical solidification curve is obtained by using the ordinate. Obtain the real part of the second harmonic signal With the driving frequency of the oscillating magnetic field The relationship between the changes is analyzed, and the peak frequency corresponding to the characteristic peak in the relationship is identified. According to the formula Calculate the absolute viscosity of the blood sample at the current moment. ,in The hydrodynamic volume of the magnetic bead; the absolute viscosity at different time points is calculated continuously. .

7. The blood analysis method as described in claim 1, characterized in that, It also includes the following steps: Place the blood sample and a predetermined number of magnetic beads in the container; An oscillating magnetic field is applied to the container to drive the magnetic bead to perform Brownian rotation; The container is illuminated with light, which passes through the area where the magnetic bead is located. The light modulated by the magnetic bead is collected to obtain light modulation information.

8. The blood analysis method as described in claim 7, characterized in that, The magnetic beads have a particle size of 50-5000 nm, and the concentration of the magnetic beads in the blood sample is [missing information]. The driving frequency of the oscillating magnetic field is 0.1-1000Hz, and the intensity is 0.1-5 mT.

9. An apparatus, characterized in that, It includes a memory, a processor, and a computer program stored in the memory and executable on the processor, the processor executing the computer program to implement the blood analysis method as described in any one of claims 1-6.

10. A system, characterized in that, Includes a magnetic field generator, a light source, a detection unit, and the device described in claim 9. The magnetic field generator is used to apply an oscillating magnetic field to a container containing a blood sample and a preset number of magnetic beads, so as to drive the magnetic beads to perform Brownian rotation. The light source is used to emit light to illuminate the container, and the light passes through the area where the magnetic bead is located; The detection unit is used to collect light that has been modulated by the magnetic beads in order to obtain light modulation information; The device is used to process the optical modulation information.

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