An apparatus and method for detecting activity of a feed enzyme preparation

Abstract

This invention belongs to the field of activity detection technology, and specifically relates to an instrument and method for detecting the activity of feed enzyme preparations. The instrument includes a detection chamber, a lid, a transmitting device, and a receiving device. The lid is disposed on the side wall of the detection chamber, and the transmitting and receiving devices are respectively fixedly disposed on the inner walls of the two sides of the detection chamber. The instrument also includes a clamping mechanism disposed on the side wall of the lid, with the clamping mechanism corresponding to the positions of the transmitting and receiving devices, and the interior of the clamping mechanism is used to hold test tubes; and a wiping mechanism disposed on the inner side wall of the detection chamber. This invention achieves convenient and stable clamping of test tubes, automated and thorough cleaning of the test tube optical path area, and uniform and precise constant-temperature heating of the reaction system. It effectively eliminates detection errors caused by fingerprints or impurities on the test tube surface, clamping deviations, and uneven temperature, significantly improving the accuracy, stability, and efficiency of feed enzyme activity detection.

Description

Technical Field

[0001] This invention belongs to the field of activity detection technology, and in particular relates to an instrument and method for detecting the activity of feed enzyme preparations. Background Technology

[0002] As a key additive for improving the nutritional value of feed and enhancing the digestibility and absorption efficiency of animals, the activity of feed enzymes directly determines the effectiveness of feed application and the economic benefits of animal husbandry. Therefore, accurate detection of enzyme activity is a core link in feed production quality control and livestock breeding selection. Currently, the mainstream methods for detecting the activity of feed enzymes in the industry all require mixing the enzyme solution with the substrate and then placing it in a test tube, placing it in a constant temperature detection environment for reaction, and capturing the changes in optical signals of the reaction solution (such as absorbance and transmittance) through a transmitting and receiving device, and then calculating the enzyme activity value, such as the feed enzyme activity detection instrument disclosed in announcement number CN119780010A.

[0003] However, during the activity detection process, when personnel handle and transfer test tubes, their hands directly contact the outer wall of the test tubes (especially the central core area through which the light path penetrates), which can easily leave behind impurities such as fingerprints, sweat, and grease. At the same time, when the test tubes are placed in the instrument, they may absorb dust, residual reagents, and other contaminants from the environment. These impurities can scatter and block the detection light path, causing the characteristic light emitted by the emitting device to fail to accurately penetrate the reaction solution, resulting in deviations in the optical signals collected by the receiving device, and ultimately causing distortion in the calculated enzyme activity value.

[0004] Therefore, a test instrument and method for detecting the activity of feed enzyme preparations are proposed. Summary of the Invention

[0005] The purpose of this invention is to address the above-mentioned problems by providing an instrument and method for detecting the activity of feed enzyme preparations.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a feed enzyme activity detection instrument, comprising a detection chamber, a chamber cover, a transmitting device, and a receiving device, wherein the chamber cover is disposed on the side wall of the detection chamber, and the transmitting device and the receiving device are respectively fixedly disposed on the inner walls of the two sides of the detection chamber, and further comprising:

[0007] A clamping mechanism is provided on the side wall of the box cover, and the clamping mechanism corresponds to the position of the transmitting device and the receiving device. The interior of the clamping mechanism is used to place test tubes.

[0008] A wiping mechanism is provided on the inner wall of the testing box, and the wiping mechanism moves in conjunction with the box cover to wipe the outer wall of the test tube in the clamping mechanism;

[0009] A water bath heating mechanism is installed on the top of the detection box, and the output end of the water bath heating mechanism is connected to the clamping mechanism. The hot water discharged by the water bath heating mechanism flows through the interior of the clamping mechanism.

[0010] The controller is fixedly installed on the top of the detection box, and the transmitting device, receiving device and water bath heating mechanism are all electrically connected to the controller.

[0011] Preferably, the clamping mechanism includes a first arc-shaped clamping plate and a second arc-shaped clamping plate located inside the testing box. Two fixing rods are symmetrically fixed between one side of the first arc-shaped clamping plate and the inner side wall of the box cover. Two moving rods are symmetrically fixed to the side wall of the second arc-shaped clamping plate. The first arc-shaped clamping plate and the second arc-shaped clamping plate are spliced ​​into a tubular structure.

[0012] Preferably, each of the two movable rods has a slider fixedly provided at the end away from the second arc-shaped clamp. The inner sidewall of the box cover has two symmetrical sliding grooves, and a sliding rod is fixedly provided inside the sliding groove. The two sliders are slidably connected to the two sliding rods respectively, and the rod walls of the two sliding rods are sleeved with springs.

[0013] Preferably, the wiping mechanism includes a fixing ring fixedly disposed at the bottom of the first arc-shaped clamping plate, a tray rotatably disposed inside the fixing ring, a rotating shaft fixedly disposed on the lower surface of the tray, a gear fixedly disposed at the lower end of the rotating shaft, a rack fixedly disposed on the inner side wall of the detection box near the box cover, the rack meshing with the gear, wiping rods fixedly disposed on both inner walls of the detection box, wiping brushes fixedly disposed at opposite ends of the two wiping rods, and wiping openings for the wiping brushes to pass through the side walls of the first arc-shaped clamping plate and the side walls of the second arc-shaped clamping plate.

[0014] Preferably, the inner walls of the upper and lower ends of the first arc-shaped clamping plate and the inner walls of the upper and lower ends of the second arc-shaped clamping plate are rotatably provided with a plurality of evenly distributed auxiliary rollers, and the upper surface of the tray is provided with an anti-slip groove.

[0015] Preferably, the water bath heating mechanism includes a heating water tank fixedly installed on the top of the detection box. Two water inlet pipes extending into the interior of the detection box are fixedly installed at the bottom of the heating water tank. The first arc-shaped clamp and the second arc-shaped clamp are both hollow structures. The ends of the two water inlet pipes away from the heating water tank are respectively fixedly connected to the top of the first arc-shaped clamp and the top of the second arc-shaped clamp. Return water pipes are fixedly installed at the bottom of the first arc-shaped clamp and the bottom of the second arc-shaped clamp. Return water tanks are fixedly installed on both sides of the detection box. The ends of the two return water pipes away from the first arc-shaped clamp and the second arc-shaped clamp are respectively fixedly connected to the two return water tanks. A temperature sensor is fixedly installed on the inner side wall of the second arc-shaped clamp.

[0016] Preferably, a magnetic block is fixedly provided on the outer edge of the box cover and magnetically adsorbed to the outer wall of the detection box, and a handle is fixedly provided in the middle of the outer edge of the box cover.

[0017] Preferably, U-shaped slide rails are fixedly provided on both inner walls of the testing box, and connecting rods are slidably provided inside the two U-shaped slide rails. The ends of the two connecting rods away from the U-shaped slide rails are fixedly connected to the inner side wall of the box cover.

[0018] A detection method for a feed enzyme activity detection instrument, the method comprising the following steps:

[0019] S1: Preparation of feed enzyme preparation. Pour a quantitative amount of enzyme powder and buffer solution into a clean container in proportion, and then slowly pour it into the test tube to ensure that the liquid level of the reaction solution in the test tube matches the light path transmission requirements.

[0020] S2: Test tube clamping: The tester grips the handle on the outside of the box lid and pulls it outward. The box lid moves the first and second arc-shaped clamping plates of the clamping mechanism to the opening of the test box. Pulling the second arc-shaped clamping plate outward causes the moving rod to slide the slider on the sliding rod and compress the spring. The test tube is placed between the first and second arc-shaped clamping plates, with the bottom of the test tube placed in the anti-slip groove on the tray. The second arc-shaped clamping plate is released, and the spring returns to its original position, causing the second arc-shaped clamping plate to cooperate with the first arc-shaped clamping plate to clamp and fix the test tube.

[0021] S3: Test tube cleaning and placement in the chamber. Push the handle to push the lid into the chamber. The clamping mechanism moves the test tube into the chamber simultaneously. During this process, the gear at the bottom of the tray meshes with the rack inside the chamber, driving the tray to rotate the test tube. The outer wall of the test tube makes full contact with the wiping brush inside the chamber, completing the wiping of impurities in the optical path detection area of ​​the test tube until the lid is magnetically fixed to the chamber by the magnetic block. The test tube is then positioned between the coaxial optical paths of the transmitting and receiving devices.

[0022] S4: Constant temperature water bath heating. The water bath heating mechanism is started by the controller. The heating tube in the heating tank heats the water to the optimal reaction temperature of the enzyme preparation. The pump introduces the hot water into the hollow structure of the first arc-shaped clamp and the second arc-shaped clamp through the inlet pipe. The temperature sensor on the inner wall of the second arc-shaped clamp collects the temperature of the test tube in real time and transmits it to the controller. The controller dynamically adjusts the power of the heating tube and the flow rate of the pump. The water after heat exchange flows back to the return tank through the return pipe to form a water cycle.

[0023] S5: Enzyme activity detection and data processing. After the reaction system in the test tube reaches the preset constant temperature and stabilizes, the transmitter and receiver are activated by the controller. The characteristic monochromatic light emitted by the transmitter is focused and passes through the wiping ports of the first and second arc-shaped clamps, vertically penetrating the reaction liquid area in the test tube. The receiver captures the penetrating light signal and transmits the data to the data analysis unit of the controller to determine the linear stage of the reaction and calculate the activity value of the feed enzyme preparation.

[0024] Compared with existing technologies, the advantages of this invention are as follows:

[0025] 1. The clamping mechanism enables convenient, stable, and adaptive clamping and fixing of test tubes. The first and second arc-shaped clamps form an elastic clamping structure through the cooperation of springs, sliders, and slide rods. The tester only needs to pull the second arc-shaped clamp outward to create a clamping space that matches the outer diameter of the test tube. After releasing, the spring's elastic potential energy enables automatic reset and clamping, eliminating the need for additional fixing operations. The clamping process is convenient and efficient. At the same time, the first and second arc-shaped clamps fit tightly against the outer wall of the test tube. Combined with the anti-slip grooves on the tray, this effectively prevents damage caused by excessive clamping or displacement during testing caused by excessive loose clamping.

[0026] 2. The wiping mechanism, based on the clamping mechanism, enables synchronized wiping. As the clamping mechanism pushes the test tube into the testing chamber along with the lid, the gear at the bottom of the tray meshes with the rack inside the testing chamber, causing the tray to rotate. This, combined with the auxiliary rollers inside the clamping mechanism, ensures that the test tube rotates at a uniform speed within the clamping range. The outer wall of the test tube can fully contact the wiping brush inside the testing chamber. Furthermore, the clamping plate of the clamping mechanism has wiping openings, ensuring thorough cleaning of the optical path detection area of ​​the test tube without any blind spots. This avoids scattering or obstruction of the detection optical path by fingerprints or impurities, improving the accuracy of optical detection. The wiping action is synchronized with the test tube insertion into the chamber, eliminating the need for additional cleaning operations and significantly improving the overall efficiency of the testing process.

[0027] 3. The water bath heating mechanism, based on the clamping mechanism, achieves uniform and constant temperature heating of the test tube. The first and second arc-shaped clamps of the clamping mechanism are hollow structures. The heating water tank of the water bath heating mechanism introduces heated hot water into the hollow interlayer of the clamps through the water inlet pipe. The hot water forms a 360° wrapping heat conduction on the outer wall of the test tube through the thermal conductivity of the first and second arc-shaped clamps. Compared with the traditional water bath method, it can achieve uniform heating of the feed enzyme preparation reaction system inside the test tube, avoiding uneven enzyme reaction rate caused by local temperature differences. At the same time, the temperature sensor on the inner wall of the second arc-shaped clamp is closely attached to the outer wall of the test tube, which can collect temperature data in real time and transmit it to the controller to realize closed-loop control of the heating temperature. Attached Figure Description

[0028] Figure 1 This is a first-view perspective perspective of a feed enzyme activity detection instrument provided by the present invention;

[0029] Figure 2 This is a second-view perspective perspective of a feed enzyme activity detection instrument provided by the present invention;

[0030] Figure 3 This is a three-dimensional view of the feed enzyme activity testing instrument provided by the present invention after the lid has been removed;

[0031] Figure 4 This is a perspective view of the connection between the box cover and the clamping mechanism in a feed enzyme preparation activity testing instrument provided by the present invention;

[0032] Figure 5 This is a three-dimensional view of the first arc-shaped clamp in a feed enzyme activity testing instrument provided by the present invention;

[0033] Figure 6 This is a three-dimensional view of the first arc-shaped clamp, the second arc-shaped clamp, and the bottom structure of a feed enzyme preparation activity testing instrument provided by the present invention;

[0034] Figure 7 This is a three-dimensional view of the first and second arc-shaped clamps after being cut open in a feed enzyme activity testing instrument provided by the present invention.

[0035] In the diagram: 1. Detection box, 2. Box cover, 3. Transmitting device, 4. Receiving device, 5. Clamping mechanism, 51. First arc-shaped clamping plate, 52. Second arc-shaped clamping plate, 53. Fixed rod, 54. Moving rod, 55. Sliding block, 56. Sliding rod, 57. Spring, 6. Wiping mechanism, 61. Fixed ring, 62. Tray, 63. Rotating shaft, 64. Gear, 65. Rack, 66. Wiping rod, 67. Wiping brush, 68. Wiping port, 69. Auxiliary roller, 610. Anti-slip groove, 7. Water bath heating mechanism, 71. Heating water tank, 72. Inlet pipe, 73. Return pipe, 74. Return water tank, 75. Temperature sensor, 8. Controller, 9. Magnetic block, 10. Handle, 11. U-shaped slide rail, 12. Connecting rod. Detailed Implementation

[0036] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0037] like Figures 1-7As shown, a feed enzyme activity testing instrument includes a testing chamber 1, a chamber cover 2, a transmitting device 3, and a receiving device 4. The chamber cover 2 is disposed on the side wall of the testing chamber 1. A magnetic block 9 is fixedly disposed on the outer edge of the outer wall of the chamber cover 2 and magnetically adsorbed to the outer wall of the testing chamber 1. A handle 10 is fixedly disposed in the middle of the outer wall of the chamber cover 2. The magnetic block 9 can increase the connection stability between the chamber cover 2 and the testing chamber 1, and the handle 10 facilitates the operation of the chamber cover 2. U-shaped slide rails 11 are fixedly disposed on both inner walls of the testing chamber 1. Connecting rods 12 are slidably disposed inside the two U-shaped slide rails 11. The ends of the two connecting rods 12 away from the U-shaped slide rails 11 are fixedly connected to the inner side wall of the chamber cover 2. During the movement of the chamber cover 2, the ends of the connecting rods 12 slide inside the U-shaped slide rails 11, increasing the stability of the movement of the chamber cover 2. The transmitting device 3 and the receiving device 4 are respectively fixedly disposed on the inner walls of both sides of the testing chamber 1.

[0038] A clamping mechanism 5 is installed on the side wall of the box cover 2, and the clamping mechanism 5 corresponds to the positions of the transmitting device 3 and the receiving device 4. The interior of the clamping mechanism 5 is used to place test tubes. The clamping mechanism 5 includes a first arc-shaped clamping plate 51 and a second arc-shaped clamping plate 52 located inside the detection box 1. Two fixing rods 53 are symmetrically fixed between one side of the first arc-shaped clamping plate 51 and the inner side wall of the box cover 2. Two moving rods 54 are symmetrically fixed on the side wall of the second arc-shaped clamping plate 52. The first arc-shaped clamping plate 51 and the second arc-shaped clamping plate 52 are spliced ​​into a tubular structure. The inner walls of the first and second arc-shaped clamps 51 and 52 are provided with a heat-conducting layer to improve the heat conduction efficiency of the test tube. The ends of the two moving rods 54 away from the second arc-shaped clamps 52 are each fixed with a slider 55. The inner wall of the box cover 2 is symmetrically provided with two sliding grooves, and a sliding rod 56 is fixedly provided inside the sliding groove. The two sliders 55 are slidably connected to the two sliding rods 56 respectively. The rod walls of the two sliding rods 56 are each fitted with a spring 57. The spring 57 enables the first arc-shaped clamps 51 and the second arc-shaped clamps 52 to have an elastic clamping function, which not only clamps the test tube, but also facilitates the assembly and disassembly of the test tube.

[0039] A wiping mechanism 6 is installed on the inner wall of the testing chamber 1. The wiping mechanism 6 moves in conjunction with the chamber lid 2 and wipes the outer wall of the test tube in the clamping mechanism 5. The wiping mechanism 6 includes a fixing ring 61 fixedly installed at the bottom of the first arc-shaped clamping plate 51. A tray 62 is rotatably mounted inside the fixing ring 61. A rotating shaft 63 is fixedly mounted on the lower surface of the tray 62. A gear 64 is fixedly mounted at the lower end of the rotating shaft 63. A rack 65 is fixedly mounted on the inner wall of the testing chamber 1, near the chamber lid 2. The rack 65 meshes with the gear 64. Wiping rods 66 are fixedly mounted on both inner walls of the testing chamber 1. The opposite ends of the two wiping rods 66 are... A wiping brush 67 is fixedly provided. The side walls of the first arc-shaped clamping plate 51 and the second arc-shaped clamping plate 52 are both provided with wiping openings 68 for the wiping brush 67 to pass through. The wiping openings 68 are designed to be at the same height as the wiping brush 67, which can ensure that the wiping brush 67 can fully contact the outer wall of the test tube. Multiple evenly distributed auxiliary rollers 69 are rotatably provided on the upper and lower inner walls of the first arc-shaped clamping plate 51 and the upper and lower inner walls of the second arc-shaped clamping plate 52. The auxiliary rollers 69 are made of high-temperature resistant rubber material, which can avoid squeezing and abrading the test tube. The upper surface of the tray 62 is provided with an anti-slip groove 610, and the inside of the anti-slip groove 610 is provided with an anti-slip texture.

[0040] A water bath heating mechanism 7 is located on top of the testing chamber 1, and its output end is connected to the clamping mechanism 5. The hot water discharged from the water bath heating mechanism 7 flows through the interior of the clamping mechanism 5. The water bath heating mechanism 7 includes a heating water tank 71 fixedly installed on top of the testing chamber 1. A heating pipe and a pump body (not shown in the figure) are installed inside the heating water tank 71. Two water inlet pipes 72 extending into the testing chamber 1 are fixedly provided at the bottom of the heating water tank 71. The first arc-shaped clamping plate 51 and the second arc-shaped clamping plate 52 are both hollow structures. The two water inlet pipes 72 are far away from the heating water tank 1. One end of the hot water tank 71 is fixedly connected to the top of the first arc-shaped clamp 51 and the top of the second arc-shaped clamp 52 respectively. The bottom of the first arc-shaped clamp 51 and the bottom of the second arc-shaped clamp 52 are both fixedly provided with return water pipes 73. Return water tanks 74 are fixedly provided on both sides of the test box 1. The ends of the two return water pipes 73 away from the first arc-shaped clamp 51 and the second arc-shaped clamp 52 are fixedly connected to the two return water tanks 74 respectively. A temperature sensor 75 is fixedly provided on the inner side wall of the second arc-shaped clamp 52. The detection end of the temperature sensor 75 can just contact the outer wall of the test tube.

[0041] The controller 8 is fixedly installed on the top of the detection box 1. The transmitting device 3, the receiving device 4 and the water bath heating mechanism 7 are all electrically connected to the controller 8.

[0042] The operating principle of this invention is described as follows: The testing personnel first prepare the feed enzyme preparation according to the preset ratio. A certain amount of enzyme powder and buffer solution are poured into a clean container in proportion. The enzyme preparation is fully dispersed by low-speed stirring to avoid clumping. After stirring, the mixture is allowed to stand for a while to remove air bubbles generated during the mixing process and ensure that the enzyme solution concentration is uniform. Then, the mixed enzyme solution is mixed with the corresponding substrate solution in proportion required for the test. The container is gently inverted 2-3 times to further ensure the uniformity of the system. The mixture is then slowly poured into the test tube, ensuring that the liquid level in the test tube meets the light path transmission requirements and avoiding the liquid level being too high or too low, which would affect the test.

[0043] After the test tube is filled with liquid, the testing personnel hold the handle 10 on the outside of the box cover 2 and pull it out smoothly. The box cover 2 drives the first arc-shaped clamp 51 and the second arc-shaped clamp 52 to move synchronously to the opening of the testing box 1. During this process, the connecting rod 12, which is fixedly connected to the box cover 2, is embedded in the U-shaped slide rail 11 and slides along the track. Through the limiting and guiding effect of the U-shaped slide rail 11, the lateral displacement during the movement is effectively offset, which significantly improves the stability of the movement of the box cover 2 and avoids collision damage.

[0044] Subsequently, the inspector used one hand to pull the second arc-shaped clamp 52 laterally outward. The moving rod 54, fixed to the side wall of the second arc-shaped clamp 52, caused the slider 55 to slide axially on the slide rod 56, simultaneously compressing the spring 57 sleeved on the slide rod 56. This created a clamping space between the first arc-shaped clamp 51 and the second arc-shaped clamp 52 that was adapted to the outer diameter of the test tube. At this point, the test tube containing the reaction solution was placed between the first arc-shaped clamp 51 and the second arc-shaped clamp 52, ensuring that the bottom of the test tube was placed in the anti-slip groove 610 on the upper surface of the tray 62. The structure fits snugly against the bottom of the test tube, and the anti-slip texture in the groove prevents the test tube from sliding or shifting. After the external force on the second arc-shaped clamp 52 is released, the compressed spring 57 releases its elastic potential energy and applies a reverse thrust to the slider 55 and the moving rod 54, causing the second arc-shaped clamp 52 to reset towards the first arc-shaped clamp 51. The first arc-shaped clamp 51 and the second arc-shaped clamp 52 fit tightly against the outer wall of the test tube, and the test tube is firmly fixed by a stable clamping force. The entire clamping process is convenient to operate and can effectively avoid damage caused by clamping the test tube too tightly or displacement caused by clamping it too loosely.

[0045] After clamping, the inspector pushes the handle 10 to slowly push the lid 2 into the test chamber 1. The first arc-shaped clamp 51 and the second arc-shaped clamp 52 simultaneously move the test tube into the test chamber 1. During this process, the gear 64 at the bottom of the tray 62 meshes with the rack 65 pre-set inside the test chamber 1. When the gear 64 rolls along the rack 65, it drives the rotating shaft 63 to rotate synchronously, thereby causing the tray 62 to rotate. Because the anti-slip groove 610 on the tray 62 is in close contact with the bottom of the test tube, and multiple rotatable auxiliary rollers 69 are provided on the inner sides of the first arc-shaped clamp 51 and the second arc-shaped clamp 52. Under the rotational driving force of the tray 62 and the guiding action of the auxiliary roller 69, the test tube rotates at a constant speed between the first arc-shaped clamp 51 and the second arc-shaped clamp 52. At the same time, during the rotation of the test tube, its outer wall comes into full contact with the wiping brushes 67 on both sides. The wiping brushes 67 are made of high-density microfiber material. The fibers are soft and do not shed. They have good adsorption and cleaning power. They can wipe the optical path detection area of ​​the test tube wall without scratching the light-transmitting surface of the test tube, and thoroughly remove residual fingerprints, grease, dust and other impurities, clearing interference for subsequent optical detection and ensuring detection accuracy.

[0046] After the lid 2 is pushed to the preset position, the magnetic block 9 fixed on the side wall of the lid 2 aligns with and tightly attracts the magnetic area on the outer wall of the test chamber 1, thus sealing and fixing the lid 2. At this time, the test tube is exactly between the coaxial optical paths of the transmitting device 3 and the receiving device 4 inside the test chamber 1, ensuring that the light can penetrate the reaction liquid area of ​​the test tube without obstruction. Then, the tester starts the heating system through the operating controller 8, and the heating tube inside the heating water tank 71 starts to work, heating the water in the tank to the optimal reaction temperature corresponding to the enzyme preparation to be tested. After the water temperature stabilizes, the pump is started, and the heated hot water is sent into the two inlet pipes 72 under the driving force of the pump. The hot water is introduced into the hollow interlayer inside the first arc-shaped clamp 51 and the second arc-shaped clamp 52 through the inlet pipes 72 respectively. The hot water quickly conducts heat to the outer wall of the test tube through the thermal conductivity of the first arc-shaped clamp 51 and the second arc-shaped clamp 52, thereby realizing the reaction of the feed enzyme preparation inside the test tube. The uniform water bath heating of the agent ensures a consistent temperature throughout the reaction system. Meanwhile, the temperature sensor 75, which is pre-set on the inner wall of the second arc-shaped clamp 52, is in close contact with the outer wall of the test tube. It can capture the temperature data of the side wall of the test tube in real time and transmit it to the controller 8 synchronously. The controller 8 dynamically compares the collected real-time temperature with the preset optimal temperature for enzyme-catalyzed reaction. If the detected temperature is lower than the set value, it will automatically adjust the power of the heating tube to increase the water temperature or increase the water flow rate of the pump to accelerate the heat transfer efficiency. If the temperature is higher than the set value, it will reduce the power of the heating tube or slow down the water flow rate. Through the closed-loop control mechanism, the heating temperature is precisely controlled to ensure that the feed enzyme preparation inside the test tube is always in a stable and uniform constant temperature reaction environment. This provides a reliable guarantee for the detection accuracy from the temperature dimension. After the heat exchange is completed, the water is continuously returned to the return water tank 74 through two return water pipes 73, forming a closed loop circulation to ensure the stability and continuity of the constant temperature effect.

[0047] Finally, after the test tube is heated to the set reaction temperature and stabilized by the closed-loop constant temperature system, the testing personnel activate the transmitting device 3 and the receiving device 4 through the controller 8. At this time, the transmitting device 3 and the receiving device 4, located on both sides of the test tube and aligned with the optical path, simultaneously enter the working state. The transmitting device 3 first filters stray light through the built-in filter, and emits characteristic monochromatic light matching the detection system from the monochromatic light source. After being focused into a parallel beam by the condenser lens, it accurately passes through the wiping port 68 on the side wall of the first arc-shaped clamp 51 and the second arc-shaped clamp 52, and is vertically projected onto the reaction liquid area in the middle of the test tube. When the light penetrates the reaction liquid, it will be specifically absorbed due to the consumption of substrate and the generation of products produced by the enzyme-catalyzed reaction. The light is absorbed or scattered, and then captured by the receiving device 4 through the light-transmitting area on the other side of the test tube. The high-sensitivity photoelectric sensor in the receiving device 4 converts the light signal into an electrical signal. After being amplified by the signal amplifier and converted by the digital-to-analog converter, the signal is transmitted in real time to the data analysis unit of the controller 8. This unit dynamically records the change curves of key optical parameters such as absorbance and transmittance. Combined with the preset detection algorithm, it determines whether the reaction is in the linear stage. Based on the signal change rate within the linear period, it accurately calculates the activity value of the feed enzyme preparation. At the same time, it automatically stores the detection data and curve graphs for easy subsequent traceability and analysis. The entire detection process does not require manual intervention and is both accurate and efficient.

[0048] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A feed enzyme activity testing instrument, comprising a testing chamber (1), a chamber cover (2), a transmitting device (3), and a receiving device (4), wherein the chamber cover (2) is disposed on the side wall of the testing chamber (1), and the transmitting device (3) and the receiving device (4) are respectively fixedly disposed on the inner walls of the two sides of the testing chamber (1), characterized in that, Also includes: A clamping mechanism (5) is provided on the side wall of the box cover (2), and the clamping mechanism (5) corresponds to the position of the transmitting device (3) and the receiving device (4). The inside of the clamping mechanism (5) is used to place test tubes. Wiping mechanism (6) is set on the inner side wall of the test box (1), and the wiping mechanism (6) is linked with the box cover (2) and wipes the outer wall of the test tube in the clamping mechanism (5); A water bath heating mechanism (7) is installed on the top of the detection box (1), and the output end of the water bath heating mechanism (7) is connected to the clamping mechanism (5). The hot water discharged by the water bath heating mechanism (7) flows through the interior of the clamping mechanism (5). The controller (8) is fixedly installed on the top of the detection box (1), and the transmitting device (3), receiving device (4) and water bath heating mechanism (7) are all electrically connected to the controller (8).

2. The feed enzyme activity detection instrument according to claim 1, characterized in that, The clamping mechanism (5) includes a first arc-shaped clamping plate (51) and a second arc-shaped clamping plate (52) located inside the detection box (1). Two fixing rods (53) are symmetrically fixed between one side of the first arc-shaped clamping plate (51) and the inner side wall of the box cover (2). Two moving rods (54) are symmetrically fixed on the side wall of the second arc-shaped clamping plate (52). The first arc-shaped clamping plate (51) and the second arc-shaped clamping plate (52) are spliced ​​into a tubular structure.

3. The feed enzyme activity detection instrument according to claim 2, characterized in that, The two movable rods (54) are each fixedly provided with a slider (55) at the end away from the second arc-shaped clamp (52). The inner sidewall of the box cover (2) is symmetrically provided with two sliding grooves, and a sliding rod (56) is fixedly provided inside the sliding groove. The two sliders (55) are slidably connected to the two sliding rods (56) respectively, and the rod walls of the two sliding rods (56) are each sleeved with a spring (57).

4. The feed enzyme activity detection instrument according to claim 3, characterized in that, The wiping mechanism (6) includes a fixing ring (61) fixedly disposed at the bottom of the first arc-shaped clamp (51). A tray (62) is rotatably disposed inside the fixing ring (61). A rotating shaft (63) is fixedly disposed on the lower surface of the tray (62). A gear (64) is fixedly disposed at the lower end of the rotating shaft (63). A rack (65) is fixedly disposed on the inner side wall of the detection box (1) and on the side near the box cover (2). The rack (65) meshes with the gear (64). Wiping rods (66) are fixedly disposed on both inner walls of the detection box (1). Wiping brushes (67) are fixedly disposed at opposite ends of the two wiping rods (66). Wiping openings (68) for the wiping brushes (67) to pass through are opened on the side walls of the first arc-shaped clamp (51) and the side walls of the second arc-shaped clamp (52).

5. The feed enzyme activity detection instrument according to claim 4, characterized in that, The upper and lower inner walls of the first arc-shaped clamp (51) and the upper and lower inner walls of the second arc-shaped clamp (52) are provided with a plurality of evenly distributed auxiliary rollers (69), and the upper surface of the tray (62) is provided with anti-slip grooves (610).

6. The feed enzyme activity detection instrument according to claim 5, characterized in that, The water bath heating mechanism (7) includes a heating water tank (71) fixedly installed on the top of the detection box (1). Two water inlet pipes (72) extending into the detection box (1) are fixedly installed at the bottom of the heating water tank (71). The first arc-shaped clamp (51) and the second arc-shaped clamp (52) are both hollow structures. The ends of the two water inlet pipes (72) away from the heating water tank (71) are fixedly connected to the top of the first arc-shaped clamp (51) and the top of the second arc-shaped clamp (52), respectively. The bottom of the first arc-shaped clamp (51) and the bottom of the second arc-shaped clamp (52) are both fixedly provided with return water pipes (73). Return water tanks (74) are fixedly installed on both sides of the detection box (1). The ends of the two return water pipes (73) away from the first arc-shaped clamp (51) and the second arc-shaped clamp (52) are fixedly connected to the two return water tanks (74), respectively. A temperature sensor (75) is fixedly installed on the inner wall of the second arc-shaped clamp (52).

7. The feed enzyme activity detection instrument according to claim 6, characterized in that, A magnetic block (9) is fixedly provided on the outer side wall edge of the box cover (2) and magnetically adsorbed to the outer wall of the detection box (1). A handle (10) is fixedly provided in the middle of the outer side wall of the box cover (2).

8. The feed enzyme activity detection instrument according to claim 7, characterized in that, The inner walls of both sides of the test box (1) are fixedly provided with U-shaped slide rails (11), and the two U-shaped slide rails (11) are slidably provided with connecting rods (12). The ends of the two connecting rods (12) away from the U-shaped slide rails (11) are fixedly connected to the inner side wall of the box cover (2).

9. A detection method applied to the feed enzyme activity detection instrument as described in claim 8, characterized in that, The method includes the following steps: S1: Preparation of feed enzyme preparation. Pour a quantitative amount of enzyme powder and buffer solution into a clean container in proportion, and then slowly pour it into the test tube to ensure that the liquid level of the reaction solution in the test tube matches the light path transmission requirements. S2: Test tube clamping. The tester grips the handle (10) on the outside of the box cover (2) and pulls it outward. The box cover (2) moves the first arc-shaped clamp (51) and the second arc-shaped clamp (52) of the clamping mechanism (5) to the opening of the test box (1). The second arc-shaped clamp (52) is pulled outward, so that the moving rod (54) drives the slider (55) to slide on the sliding rod (56) and compress the spring (57). The test tube is placed between the first arc-shaped clamp (51) and the second arc-shaped clamp (52), and the bottom of the test tube is placed in the anti-slip groove (610) on the tray (62). The second arc-shaped clamp (52) is released, and the spring (57) resets, causing the second arc-shaped clamp (52) to cooperate with the first arc-shaped clamp (51) to clamp and fix the test tube. S3: Test tube cleaning and placement in the box. Push the handle (10) to push the box cover (2) into the test box (1). The clamping mechanism (5) drives the test tube to move into the test box (1) simultaneously. During this process, the gear (64) at the bottom of the tray (62) meshes with the rack (65) on the inside of the test box (1), driving the tray (62) to rotate the test tube. The outer wall of the test tube fully contacts the wiping brush (67) inside the test box (1) to complete the wiping of the test tube optical path detection area until the box cover (2) is magnetically fixed to the test box (1) by the magnetic block (9). The test tube is located between the coaxial optical path of the transmitting device (3) and the receiving device (4). S4: Constant temperature water bath heating. The water bath heating mechanism (7) is started by the controller (8). The heating tube in the heating water tank (71) heats the internal water to the optimal reaction temperature corresponding to the enzyme preparation to be tested. The pump body introduces hot water into the hollow structure of the first arc-shaped clamp (51) and the second arc-shaped clamp (52) through the inlet pipe (72). The temperature sensor (75) on the inner wall of the second arc-shaped clamp (52) collects the temperature of the test tube in real time and transmits it to the controller (8). The controller (8) dynamically adjusts the power of the heating tube and the flow rate of the pump body. The water after heat exchange flows back to the return water tank (74) through the return water pipe (73) to form a water cycle. S5: Enzyme activity detection and data processing. After the reaction system in the test tube reaches the preset constant temperature and stabilizes, the transmitter (3) and receiver (4) are started by the controller (8). The characteristic monochromatic light emitted by the transmitter (3) is focused and passes through the wiping port (68) of the first arc-shaped clamp (51) and the second arc-shaped clamp (52), and vertically penetrates the reaction liquid area in the test tube. The receiver (4) captures the light signal after penetration and transmits the data to the data analysis unit of the controller (8), determines the linear stage of the reaction and calculates the activity value of the feed enzyme preparation.

Images