Application of a VviGAI gene mutant and a recombinant vector in dwarf grape breeding
By using gene editing technology, VviGAI gene mutants and recombinant vectors, the problem of low grape breeding efficiency in existing technologies has been solved, achieving a stable reduction in grapevine height and a shortening of the breeding cycle, providing compact grape materials suitable for greenhouse cultivation and potted plants.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- QINGHAI UNIVERSITY
- Filing Date
- 2026-04-24
- Publication Date
- 2026-06-19
AI Technical Summary
Current technologies cannot clearly silence, overexpress, or mutate the VviGAI gene to obtain the desired dwarf grape phenotype, resulting in low grape breeding efficiency, long cycles, and unstable traits.
By using gene editing technology, VviGAI gene mutants and recombinant vectors, especially CRISPR/Cas9 technology, the VviGAI gene of grapes was edited to achieve targeted reduction of grapevine height. PCR amplification and sequencing analysis were performed using VviGAI gene mutant primers and buffer reagents to obtain stable dwarfing traits.
It has achieved a stable reduction in grapevine height, shortened the breeding cycle, improved breeding efficiency, obtained compact grape materials suitable for greenhouse cultivation and pot cultivation, applicable to a variety of grape varieties, and significantly improved the safety and trait orientation of breeding.
Smart Images
Figure CN122235162A_ABST