Non-invasive device and method for detecting in a body fluid a fatty acid receptor agonist antagonist

Abstract

This invention discloses a non-invasive detection device and method for fatty acid receptor agonist antagonists in body fluids, relating to the field of biomedical detection and automation equipment. The non-invasive detection device is configured as a high-throughput liquid processing workstation for solid-phase extraction pretreatment of body fluid samples. The workstation includes a cabinet, a drawer-type sample loading module, a reciprocating oscillation module, a pressure extraction module, and a sample loading module. The cabinet is a sealed box with a control console on one side and a horizontal partition inside, dividing the cabinet cavity into an upper processing chamber and a lower functional chamber. X-axis sliding stages are provided at both the front and rear ends of the horizontal partition. This non-invasive detection device and method integrates the horizontal reciprocating oscillation module, the sample loading module, and the pressure extraction module, further realizing a fully enclosed, standardized, and streamlined operation from sample entry to eluent collection. In particular, it optimizes the extraction pressure and oscillation mixing effect for the physicochemical properties of fatty acid receptor drugs.

Description

Technical Field

[0001] This invention relates to biomedical detection and automation equipment technology, specifically to a non-invasive detection device and method for fatty acid receptor agonist antagonists in body fluids. Background Technology

[0002] Fatty acid receptors (such as GPR40, GPR120, and GPR84), as important members of the G protein-coupled receptor family, play a crucial role in regulating physiological processes such as insulin secretion, inflammatory responses, and energy metabolism. They have become novel drug targets for the treatment of metabolic diseases such as type 2 diabetes, obesity, and non-alcoholic fatty liver disease. Agonist or antagonist drugs targeting these targets require systematic pharmacokinetic evaluation in preclinical and clinical trials, with accurate detection of the drug in body fluids being essential.

[0003] Traditional detection methods primarily rely on blood samples, which are invasive procedures with poor patient compliance and unsuitable for long-term monitoring. Non-invasive bodily fluid samples such as urine and saliva, due to their ease of acquisition and repeatability, have become ideal for pharmacokinetic studies and therapeutic drug monitoring. However, non-invasive bodily fluid samples present challenges such as complex matrices, extremely low target drug concentrations (often ng / mL or even pg / mL), and numerous endogenous interfering substances, placing extremely high demands on the purification efficiency and enrichment capabilities of pretreatment techniques.

[0004] Currently, the pretreatment of such trace drugs mainly relies on solid-phase extraction technology. However, existing technologies have significant shortcomings:

[0005] First, the pretreatment process is highly dependent on manual operation, including multiple steps such as sample transfer, reagent addition, SPE column activation, sample loading, rinsing, and elution. The operation is cumbersome and time-consuming, and the labor intensity of personnel is high.

[0006] Secondly, manual operation has poor reproducibility and is prone to human error, which affects the accuracy and reliability of the test results.

[0007] Furthermore, traditional methods have low throughput, making it difficult to meet the efficiency requirements of clinical research or large-scale sample testing;

[0008] Finally, existing devices are often functionally generalized and have not been optimized and integrated to address the specific physicochemical properties (such as lipophilicity, easy adsorption, and the need for specific pH conditions) and pretreatment requirements of fatty acid receptor drugs in non-invasive body fluid samples.

[0009] Therefore, there is an urgent need in the field for an automated pretreatment device and method specifically for the non-invasive detection of fatty acid receptor agonists / antagonists. Summary of the Invention

[0010] The purpose of this invention is to provide a non-invasive detection device and method for fatty acid receptor agonist antagonists in body fluids, in order to solve the problems of cumbersome pretreatment operations, low efficiency, poor reproducibility, and lack of dedicated equipment for high-throughput, automated purification and enrichment of complex non-invasive body fluid samples in the prior art.

[0011] To achieve the above objectives, the present invention provides the following technical solution: a non-invasive detection device for fatty acid receptor agonist antagonists in body fluids. The non-invasive detection device is configured as a high-throughput liquid processing workstation for solid-phase extraction pretreatment of body fluid (urine) samples. The workstation includes a cabinet, a drawer-type sample loading module, a reciprocating oscillation module, a pressure extraction module, and a sample loading module, wherein:

[0012] The cabinet is a sealed box with a control console on one side and horizontal partitions inside. The horizontal partitions divide the cabinet's interior into an upper processing chamber and a lower functional chamber.

[0013] X-axis slides are provided at both the front and rear ends of the horizontal partition. The drawer-type loading module, reciprocating oscillation module, and sample loading module are all installed in the processing chamber. The drawer-type loading module includes a loading stage and a Y-axis slide. The loading stage is located on the upper side of the horizontal partition and its front and rear ends are slidably connected to the X-axis slide. A Y-axis slide is provided on the side of the loading stage. The reciprocating oscillation module is located on the upper side of the loading stage and is connected to the Y-axis slide. The reciprocating oscillation module is a horizontal reciprocating oscillation platform with clamping arms. The reciprocating oscillation module is used to clamp the microplate and perform periodic oscillation with controllable amplitude and frequency in the horizontal plane.

[0014] The sample dispensing module is mounted on the upper side of the processing chamber via a transverse truss. The sample dispensing module is used to perform liquid dispensing and transfer operations. The sample dispensing module includes a sample dispensing lifting slide, a sample dispensing pump head, and multiple reagent containers. The sample dispensing lifting slide is connected to the transverse truss via an angle steel bracket, and the sample dispensing pump head is installed on the sliding end of the sample dispensing lifting slide.

[0015] Furthermore, the dispensing pump head is a liquid dispensing end that integrates a multi-channel high-precision syringe pump. Multiple reagent containers are integrated into the outer protective cover at the top of the dispensing pump head and connected to the dispensing pump head through tubing to form a liquid circuit system that automatically adds reagents to and mixes body fluid samples in the microplate.

[0016] Furthermore, the pressure extraction module is installed in the functional cavity at the bottom of the cabinet. The pressure extraction module applies positive or negative pressure to the solid phase extraction plate located directly above it to drive the liquid to complete the activation, loading, rinsing and elution steps of solid phase extraction. The multi-channel sealed top of the pressure extraction module extends out of the horizontal partition and docks with the bottom of the microporous plate located in the processing cavity.

[0017] Furthermore, the lower part of the wells in the microplate is filled with a solid-phase extraction adsorbent, and a porous filter plate is provided at the bottom of the microplate. This integrates the traditionally separated sample container and solid-phase extraction column into a standardized, disposable consumable. Users only need to place this integrated microplate, and the device automatically completes all pretreatment, greatly simplifying the operation process and completely avoiding the errors and contamination risks associated with manual sample transfer and extraction column replacement.

[0018] Furthermore, transparent, openable observation windows are provided on the front and top sides of the processing chamber.

[0019] Furthermore, the control console is connected to the side of the cabinet via a cantilever bracket. The control console integrates a touch screen, a physical emergency stop button, and a program start / stop switch. It is also connected to the controllers of the drawer-type loading module, reciprocating oscillation module, pressure extraction module, and sample dispensing module via a data bus to realize human-machine interaction, parameter setting, program calling, and real-time monitoring of equipment operating status.

[0020] Furthermore, a sealing through-hole array is provided on the horizontal partition corresponding to the position of the pressure extraction module. The sealing top of the pressure extraction module can be vertically passed through the through-hole array and rise into the processing chamber to form a sealed connection with the bottom of the microporous plate.

[0021] A non-invasive method for detecting fatty acid receptor agonist antagonists in body fluids, the method being implemented using the aforementioned non-invasive detection device, includes the following steps:

[0022] S1: Place the microplate containing the body fluid sample as the sample plate on the stage of the drawer-type sample carrier module, and call the preset fatty acid receptor drug detection program through the control console.

[0023] S2: The clamping arm of the reciprocating oscillation module automatically clamps and fixes the sample plate, and starts oscillation according to the program instructions to initially mix the body fluid in the sample plate.

[0024] S3: The sample pump head of the sample addition module moves above the sample plate and adds stable isotope internal standard solution and pH adjustment buffer to each sample well according to the program; after the sample addition is completed, the reciprocating oscillation module starts again to oscillate and mix the mixture.

[0025] S4: The drawer-type loading module drives the loading stage to move along the X and Y directions, transferring the sample plate out of the sample loading station;

[0026] S5: The multi-channel sealing head of the pressure extraction module rises, passes through the sealing through-hole array of the horizontal partition, and forms a seal with the bottom of the solid phase extraction plate; subsequently, the program automatically controls the pressure and completes the following steps in sequence:

[0027] S5a: Apply negative pressure to drive the activation solvent through the extraction plate packing;

[0028] S5b: Maintain negative pressure to drive the sample solution through the packing material at a constant flow rate, allowing the target analyte to be adsorbed;

[0029] S5c: Switch pressure to drive the eluent through the packing to remove impurities;

[0030] S5d: Apply positive pressure to drive the elution solvent through the packing material, eluting the enriched target analytes into the pre-positioned clean collection plate below;

[0031] S6: The sealing top of the pressure extraction module descends and resets, the drawer-type loading module removes the solid phase extraction plate that has completed extraction, and transfers the collection plate containing the target eluent to the output position;

[0032] S7: Remove the collection plate from the device and use it directly for subsequent liquid chromatography-tandem mass spectrometry analysis to obtain the accurate concentration of fatty acid receptor agonists or antagonists in body fluids.

[0033] Compared with existing technologies, the non-invasive detection device and method for fatty acid receptor agonist antagonists in body fluids provided by this invention, at the device level, integrates a horizontal reciprocating oscillation module, a sample loading module, and a pressure extraction module. This integration replaces manual operation and further realizes a fully enclosed, standardized, streamlined operation from sample entry to eluent collection. In particular, it optimizes the extraction pressure and oscillation mixing effect based on the physicochemical properties of fatty acid receptor drugs. Specific technical effects include the following:

[0034] 1. The device is based on a microplate design, which enables high-throughput parallel processing, greatly improving the detection throughput and making it suitable for large-scale clinical research or routine monitoring.

[0035] 2. The method optimizes the extraction packing, pH adjustment steps, elution solvent, and programmed pressure parameters to address the lipophilicity and easy adsorption characteristics of fatty acid receptor drugs. Combined with the isotope internal standard method, it effectively reduces the matrix effect, improves the enrichment ability and anti-interference ability of trace targets, and ensures the accuracy and reliability of subsequent LC-MS / MS analysis. Attached Figure Description

[0036] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0037] Figure 1 This is a schematic diagram of the overall structure of the present invention. Figure 1 ;

[0038] Figure 2 This is a schematic diagram of the overall structure of the present invention. Figure 2;

[0039] Figure 3 This is a schematic diagram of the structure of the horizontal partition, the X-axis slide, and the drawer-type loading module in Embodiment 1 of the present invention;

[0040] Figure 4 This is a schematic diagram of the sample loading module in Embodiment 1 of the present invention;

[0041] Figure 5 This is a schematic diagram of the structure of Embodiment 2 of the present invention.

[0042] Explanation of reference numerals in the attached figures:

[0043] 1. Cabinet; 101. Processing chamber; 102. Functional chamber; 103. Observation window; 2. Horizontal partition; 3. X-axis slide; 4. Drawer-type loading module; 5. Reciprocating oscillation module; 6. Sample loading module; 601. Sample loading lifting slide; 602. Sample loading pump head; 603. Outer protective cover; 7. Pressure extraction module; 8. Microplate; 9. Control console. Detailed Implementation

[0044] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0045] As attached Figure 1 To be continued Figure 4 As shown:

[0046] Example 1:

[0047] The present invention provides a non-invasive detection device for fatty acid receptor agonist antagonists in body fluids. The non-invasive detection device is configured as a high-throughput liquid processing workstation for solid-phase extraction pretreatment of body fluid (urine) samples. The workstation includes a cabinet 1, a drawer-type material carrier module 4, a reciprocating oscillation module 5, and a sample loading module 6.

[0048] 1. In one embodiment of the present invention, the cabinet 1 is a sealed box with a control console 9 on one side and a horizontal partition 2 inside. The horizontal partition 2 divides the inner cavity of the cabinet 1 into an upper processing cavity 101 and a lower functional cavity 102.

[0049] 2. In one embodiment of the present invention, X-axis slides 3 are provided at both the front and rear ends of the horizontal partition 2. The drawer-type loading module 4, the reciprocating oscillation module 5, and the sample loading module 6 are all installed in the processing cavity 101. The drawer-type loading module 4 includes a loading stage and a Y-axis slide. The loading stage is located on the upper side of the horizontal partition 2 and its front and rear ends are slidably connected to the X-axis slides 3. A Y-axis slide is provided on the side end of the loading stage. The reciprocating oscillation module 5 is located on the upper side of the loading stage and is connected to the Y-axis slide. The reciprocating oscillation module 5 is a horizontal reciprocating oscillation platform with clamping arms. The reciprocating oscillation module 5 is used to clamp the microporous plate 8 and perform periodic oscillations with controllable amplitude and frequency in the horizontal plane.

[0050] 3. In one embodiment of the present invention, the sample dispensing module 6 is mounted on the upper side of the processing chamber 101 via a transverse truss. The sample dispensing module 6 is used to perform liquid dispensing and transfer operations. The sample dispensing module 6 includes a sample dispensing lifting slide 601, a sample dispensing pump head 602, and multiple reagent containers. The sample dispensing lifting slide 601 is connected to the transverse truss via an angle steel bracket. The sample dispensing pump head 602 is disposed on the sliding end of the sample dispensing lifting slide 601. The sample dispensing pump head 602 is a liquid dispensing end that integrates a multi-channel high-precision syringe pump. Multiple reagent containers are integrated in the outer protective cover 603 at the upper end of the sample dispensing pump head 602 and are connected to the sample dispensing pump head 602 via pipelines to form a liquid circuit system for automatically adding reagents and mixing body fluid samples in the microplate 8.

[0051] 4. In one embodiment of the present invention, a transparent and openable observation window 103 is also provided on the front and top sides of the processing cavity 101.

[0052] 5. In one embodiment of the present invention, the control console 9 is connected to the side of the cabinet 1 via a cantilever bracket. The control console 9 integrates a touch screen, a physical emergency stop button and a program start / stop switch, and is connected to the controllers of the drawer-type loading module 4, the reciprocating oscillation module 5, the pressure extraction module 7 and the sample loading module 6 via a data bus, so as to realize human-machine interaction, parameter setting, program calling and real-time monitoring of equipment operating status.

[0053] Working principle: Example 1 provides a complete automated liquid processing platform. The user places the microplate 8 containing the urine sample on the stage, and after calling the program through the control console 9, the device can automatically complete standardized pretreatment steps such as sample positioning, precise addition of reagents, and vortex mixing.

[0054] As attached Figure 1 To be continued Figure 5 As shown:

[0055] Example 2:

[0056] This embodiment is basically the same as the previous embodiment, except that it also includes a pressure extraction module 7. The pressure extraction module 7 is installed in the functional cavity 102 at the bottom of the cabinet 1. The pressure extraction module 7 applies positive or negative pressure to the solid phase extraction plate located directly above it to drive the liquid to complete the activation, loading, rinsing and elution steps of solid phase extraction. The multi-channel sealed top of the pressure extraction module 7 extends out of the horizontal partition 2 and docks with the bottom of the microporous plate 8 located in the processing cavity 101.

[0057] 1. In one embodiment of the present invention, the lower part of the wells of the microplate 8 is filled with a solid-phase extraction adsorbent, and a porous filter plate is provided at the bottom of the microplate 8. The traditionally separated sample container and solid-phase extraction column are integrated into a standardized, disposable consumable. Users only need to place this integrated microplate 8, and the device can automatically complete all pretreatment, greatly simplifying the operation process and completely avoiding the error and contamination risks caused by manual sample transfer and extraction column replacement.

[0058] 2. In one embodiment of the present invention, a sealing through-hole array is provided on the horizontal partition 2 at the position corresponding to the pressure extraction module 7. The sealing top of the pressure extraction module 7 can be vertically passed through the through-hole array and rise into the processing chamber 101 to form a sealed connection with the bottom of the microporous plate 8.

[0059] Working Principle: Based on the automated liquid processing achieved in Example 1, Example 2 integrates the pressure extraction module 7 and the dedicated microplate 8 to achieve fully automated and programmed execution of the solid-phase extraction step. Specifically, after the device completes sample pretreatment and mixing, the drawer-type loading module 4 moves the microplate 8 carrying the sample to the pressure extraction station. The sealing head of the pressure extraction module 7 rises to complete the docking seal, and then, according to a preset method, precisely controlled negative and positive pressures are applied sequentially, driving the liquid inside the plate to complete key steps such as solid-phase extraction column activation, sample loading and target adsorption, impurity rinsing, and target elution. The entire process requires no manual intervention, automatically outputting the purified, enriched, and collected target elution solution in a clean plate, achieving a fully enclosed automated production line operation. This is particularly suitable for high-throughput, high-reproducibility, non-invasive pretreatment for trace drugs such as fatty acid receptor agonists / antagonists.

[0060] In conjunction with the non-invasive detection devices described in Embodiments 1 and 2 above, the present invention also provides a non-invasive detection method for fatty acid receptor agonist antagonists in body fluids, comprising the following steps:

[0061] S1: Place the microplate 8 containing the body fluid sample as a sample plate on the stage of the drawer-type material carrier module 4, and call the preset fatty acid receptor drug detection program through the control console 9.

[0062] S2: The clamping arm of the reciprocating oscillation module 5 automatically clamps and fixes the sample plate, and starts oscillation according to the program instructions to initially mix the body fluid in the sample plate.

[0063] S3: The sample pump head 602 of the sample addition module 6 moves to the top of the sample plate and adds stable isotope internal standard solution and pH adjustment buffer to each sample well according to the program; after the sample addition is completed, the reciprocating oscillation module 5 is started again to oscillate and mix the mixture.

[0064] S4: Drawer-type loading module 4 drives the loading stage to move along the X and Y directions, transferring the sample plate out of the sample loading station;

[0065] S5: The multi-channel sealing head of the pressure extraction module 7 rises, passes through the sealing through-hole array of the horizontal partition 2, and forms a seal with the bottom of the solid phase extraction plate; subsequently, the program automatically controls the pressure and completes the following steps in sequence:

[0066] S5a: Apply negative pressure to drive the activation solvent through the extraction plate packing;

[0067] S5b: Maintain negative pressure to drive the sample solution through the packing material at a constant flow rate, allowing the target analyte to be adsorbed;

[0068] S5c: Switch pressure to drive the eluent through the packing to remove impurities;

[0069] S5d: Apply positive pressure to drive the elution solvent through the packing material, eluting the enriched target analytes into the pre-positioned clean collection plate below;

[0070] S6: The sealing top of the pressure extraction module 7 descends and resets, the drawer-type loading module 4 removes the solid phase extraction plate that has completed extraction, and transfers the collection plate containing the target eluent to the output position.

[0071] S7: Remove the collection plate from the device and use it directly for subsequent liquid chromatography-tandem mass spectrometry analysis to obtain the accurate concentration of fatty acid receptor agonists or antagonists in body fluids.

[0072] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A non-invasive detection device for fatty acid receptor agonist antagonists in body fluids, wherein the non-invasive detection device is configured as a high-throughput liquid processing workstation for solid-phase extraction pretreatment of body fluid samples, characterized in that, The workstation includes a cabinet (1), a drawer-type loading module (4), a reciprocating oscillation module (5), a pressure extraction module (7), and a sample loading module (6), wherein: The cabinet (1) is a sealed box with a control console (9) on one side and a horizontal partition (2) inside. The horizontal partition (2) divides the inner cavity of the cabinet (1) into an upper processing cavity (101) and a lower functional cavity (102). X-axis slides (3) are provided at both the front and rear ends of the horizontal partition (2). The drawer-type loading module (4), the reciprocating oscillation module (5) and the sample loading module (6) are all installed in the processing chamber (101). The drawer-type loading module (4) includes a loading stage and a Y-axis slide. The loading stage is located on the upper side of the horizontal partition (2) and its front and rear ends are slidably connected to the X-axis slide (3). A Y-axis slide is provided on the side end of the loading stage. The reciprocating oscillation module (5) is located on the upper side of the loading stage and is connected to the Y-axis slide. The reciprocating oscillation module (5) is a horizontal reciprocating oscillation platform with clamping arms. The reciprocating oscillation module (5) is used to clamp the microporous plate (8) and perform periodic oscillation with controllable amplitude and frequency in the horizontal plane. The sample dispensing module (6) is mounted on the upper side of the processing chamber (101) via a transverse truss. The sample dispensing module (6) is used to perform liquid dispensing and transfer operations. The sample dispensing module (6) includes a sample dispensing lifting slide (601), a sample dispensing pump head (602), and multiple reagent containers. The sample dispensing lifting slide (601) is connected to the transverse truss via an angle steel bracket. The sample dispensing pump head (602) is installed on the sliding end of the sample dispensing lifting slide (601).

2. The non-invasive detection device for fatty acid receptor agonist antagonists in body fluids according to claim 1, characterized in that, The sample dispensing pump head (602) is a liquid dispensing end of an internally integrated multi-channel high-precision injection pump. Multiple reagent containers are integrated in the outer protective cover (603) at the upper end of the sample dispensing pump head (602) and connected to the sample dispensing pump head (602) through pipelines to form a liquid circuit system for automatically adding reagents and mixing body fluid samples in the microplate (8).

3. The non-invasive detection device for fatty acid receptor agonist antagonists in body fluids according to claim 1, characterized in that, The pressure extraction module (7) is installed in the functional cavity (102) at the bottom of the cabinet (1). The pressure extraction module (7) applies positive or negative pressure to the solid phase extraction plate located directly above it to drive the liquid to complete the activation, loading, rinsing and elution steps of solid phase extraction. The multi-channel sealed top of the pressure extraction module (7) extends out of the horizontal partition (2) and docks with the bottom of the microporous plate (8) located in the processing cavity (101).

4. The non-invasive detection device for fatty acid receptor agonist antagonists in body fluids according to claim 1, characterized in that, The lower part of the pores of the microporous plate (8) is filled with a solid phase extraction adsorbent, and a porous filter plate is provided at the bottom of the microporous plate (8).

5. The non-invasive detection device for fatty acid receptor agonist antagonists in body fluids according to claim 1, characterized in that, The processing chamber (101) is also provided with a transparent, openable observation window (103) on the front and top sides.

6. The non-invasive detection device for fatty acid receptor agonist antagonists in body fluids according to claim 1, characterized in that, The console (9) is connected to the side of the cabinet (1) via a cantilever bracket. The console (9) integrates a touch screen, a physical emergency stop button and a program start / stop switch, and is connected to the controllers of the drawer-type loading module (4), the reciprocating oscillation module (5), the pressure extraction module (7) and the sample loading module (6) via a data bus.

7. The non-invasive detection device for fatty acid receptor agonist antagonists in body fluids according to claim 1, characterized in that, The horizontal partition (2) is provided with a sealing through-hole array at the position corresponding to the pressure extraction module (7). The sealing top of the pressure extraction module (7) can pass vertically through the through-hole array and rise into the processing chamber (101) to form a sealed connection with the bottom of the microporous plate (8).

8. A non-invasive method for detecting fatty acid receptor agonist antagonists in body fluids, characterized in that, This method is implemented based on the non-invasive detection device according to any one of claims 1-7, and includes the following steps: S1: Place the microplate (8) containing the body fluid sample as a sample plate on the stage of the drawer-type loading module (4), and call the preset fatty acid receptor drug detection program through the control console (9). S2: The clamping arm of the reciprocating oscillation module (5) automatically clamps and fixes the sample plate, and starts oscillation according to the program instructions to initially mix the body fluid in the sample plate; S3: The sample pump head (602) of the sample addition module (6) moves to the top of the sample plate and adds stable isotope internal standard solution and pH adjustment buffer to each sample well according to the program; after the sample addition is completed, the reciprocating oscillation module (5) is started again to oscillate and mix the mixture. S4: The drawer-type loading module (4) drives the loading stage to move along the X and Y directions, transferring the sample plate out of the sample loading station; S5: The multi-channel sealing head of the pressure extraction module (7) rises, passes through the sealing through-hole array of the horizontal partition (2), and forms a seal with the bottom of the solid phase extraction plate; subsequently, the program automatically controls the pressure and completes the following steps in sequence: S5a: Apply negative pressure to drive the activation solvent through the extraction plate packing; S5b: Maintain negative pressure to drive the sample solution through the packing material at a constant flow rate, allowing the target analyte to be adsorbed; S5c: Switch pressure to drive the eluent through the packing to remove impurities; S5d: Apply positive pressure to drive the elution solvent through the packing material, eluting the enriched target analytes into the pre-positioned clean collection plate below; S6: The sealing top of the pressure extraction module (7) descends and resets, the drawer-type loading module (4) removes the solid phase extraction plate that has completed extraction, and transfers the collection plate containing the target eluent to the output position; S7: Remove the collection plate from the device and use it directly for subsequent liquid chromatography-tandem mass spectrometry analysis to obtain the accurate concentration of fatty acid receptor agonists or antagonists in body fluids.

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