A detection patch

Abstract

This application provides a detection patch, relating to the field of detection technology. The detection patch includes a base layer and a colorimetric detection layer. The colorimetric detection layer is disposed on one side of the base layer in the thickness direction; the detection layer includes a carrier and a colorimetric detection part, the colorimetric detection part being disposed on the side of the carrier opposite to the base layer. Both the base layer and the colorimetric detection layer are paper base layers. By using paper base layers for both the base layer and the colorimetric detection layer, the paper base layer can be processed through simple cutting, folding, and lamination processes. This makes the base layer and the colorimetric detection layer easy to manufacture, reducing the complexity of the detection patch preparation. Furthermore, the colorimetric detection layer indicates the presence or concentration change of substances in sweat through color changes. The paper base layer colorimetric detection layer can quickly absorb and fix sweat and chemical reagents, and the paper base layer colorimetric detection layer contributes to the uniformity of chemical reagent coating, helping to improve the detection accuracy and consistency of the detection patch.

Description

Technical Field

[0001] This application relates to the field of testing technology, and in particular to a testing patch. Background Technology

[0002] Real-time monitoring of physiological indicators related to sports and health helps athletes understand their metabolic status and exercise adaptation, which in turn helps them with subsequent training arrangements, physical recovery, and nutritional supplementation.

[0003] In related technologies, methods for detecting physiological indicators include electrochemical detection and colorimetric methods. Electrochemical detection is based on electrochemical reactions, detecting specific chemical substances by measuring changes in current, voltage, or resistance. Colorimetric detection is based on the reaction of specific chemical reagents with target substances in sweat (such as electrolytes, lactic acid, glucose, etc.), resulting in color changes. By measuring these color changes, the concentration of the target substance can be inferred.

[0004] However, the test strips in the relevant technologies have complex preparation issues. Summary of the Invention

[0005] This application provides a detection patch that solves the problem of complex patch preparation by setting the base layer and colorimetric detection layer as a paper base layer.

[0006] This application provides a testing patch, including a base layer and a colorimetric testing layer. The colorimetric testing layer is disposed on one side of the base layer in the thickness direction; the colorimetric testing layer includes a carrier and a colorimetric testing part, the colorimetric testing part being disposed on the side of the carrier opposite to the base layer.

[0007] Both the base layer and the colorimetric detection layer are paper base layers.

[0008] In some embodiments of this application, the detection patch further includes a permeation layer disposed between the colorimetric detection layer and the base layer.

[0009] In some embodiments of this application, the detection patch further includes a transparent overlay layer disposed on the side of the colorimetric detection layer away from the substrate.

[0010] In some embodiments of this application, the detection patch further includes an isolation layer. The isolation layer is disposed between the permeation layer and the base layer.

[0011] The isolation layer is provided with mounting holes, and the permeable layer contacts the base layer through the mounting holes.

[0012] In some embodiments of this application, the isolation layer and / or the permeable layer are paper base layers.

[0013] In some embodiments of this application, the number of colorimetric detection units is multiple.

[0014] And / or, multiple colorimetric detection units are spaced apart along the extension plane of the carrier.

[0015] And / or, multiple colorimetric detection units are made of different materials and are used to detect different substances.

[0016] In some embodiments of this application, the permeable layer has a first pore, and the pore size D1 of the first pore satisfies: 8μm≤D1≤15μm.

[0017] In some embodiments of this application, the thickness of the permeation layer is P1, where P1 satisfies: 0.15≤P1≤0.25mm.

[0018] In some embodiments of this application, the colorimetric detection unit has a second pore, and the pore diameter D2 of the second pore satisfies: 0.5μm≤D2≤1.5μm.

[0019] In some embodiments of this application, the thickness of the colorimetric detection section is P2, where P2 satisfies: 1.5mm≤P2≤2.5mm.

[0020] In some embodiments of this application, the thickness of the isolation layer is P3, where P3 satisfies: 0.05mm≤P3≤0.15mm.

[0021] In some embodiments of this application, at least one of the following limitations is satisfied:

[0022] The materials for the transparent overlay include polyethylene terephthalate, polyethylene, and polypropylene.

[0023] The carrier includes silicone paper.

[0024] The colorimetric detection unit includes glass fiber filter paper.

[0025] The permeation layer consists of cotton fiber filter paper.

[0026] The insulating layer includes silicone paper.

[0027] The base layer includes release paper.

[0028] In some embodiments of this application, the colorimetric detection unit includes a glucose detection unit, a lactic acid detection unit, a urea detection unit, and a pH detection unit.

[0029] The glucose detection unit, lactic acid detection unit, urea detection unit, and pH detection unit are arranged in an array on the carrier.

[0030] In some embodiments of this application, the materials of the glucose detection unit include a glucose catalyst, a peroxide catalyst, a colorimetric reagent, and a pH reagent.

[0031] In some embodiments of this application, the glucose catalyst includes glucose oxidase.

[0032] In some embodiments of this application, the peroxide catalyst includes horseradish peroxidase.

[0033] In some embodiments of this application, the colorimetric agent includes 3,3'-dimethoxybenzidine.

[0034] In some embodiments of this application, the pH reagent includes a citrate-disodium hydrogen phosphate buffer solution.

[0035] In some embodiments of this application, the materials of the lactic acid detection unit include a lactic acid catalyst, a peroxide catalyst, a colorimetric reagent, and a pH reagent.

[0036] In some embodiments of this application, the lactic acid catalyst includes lactate oxidase.

[0037] In some embodiments of this application, the peroxide catalyst includes peroxidase.

[0038] In some embodiments of this application, the colorimetric agent includes 4-aminoantipyrine.

[0039] In some embodiments of this application, the pH reagent includes a citrate-disodium hydrogen phosphate buffer solution.

[0040] In some embodiments of this application, the materials of the urea detection unit include p-dimethylaminobenzaldehyde, sulfate, and pH reagent.

[0041] In some embodiments of this application, the sulfate includes potassium hydrogen sulfate.

[0042] In some embodiments of this application, the pH reagent includes a citrate-disodium hydrogen phosphate buffer solution.

[0043] In some embodiments of this application, the material of the pH detection unit includes a pH indicator and a stabilizer.

[0044] In some embodiments of this application, the pH indicator includes alizarin red.

[0045] In some embodiments of this application, the stabilizer includes hexadecyltrimethylammonium bromide.

[0046] In some embodiments of this application, the materials of the glucose detection unit include glucose oxidase, horseradish peroxidase, and 3,3'-dimethoxybenzidine; the mass ratio of glucose oxidase, horseradish peroxidase, and 3,3'-dimethoxybenzidine is (4-30):(1-20):(1-8).

[0047] In some embodiments of this application, the materials of the lactate detection unit include lactate oxidase, peroxidase, and 4-aminoantipyrine; the mass ratio of lactate oxidase, peroxidase, and 4-aminoantipyrine is (1-20):(2-40):(7-30).

[0048] In some embodiments of this application, the materials of the urea detection unit include p-dimethylaminobenzaldehyde and potassium bisulfate; the mass ratio of p-dimethylaminobenzaldehyde to potassium bisulfate is (3-20):(1-40).

[0049] In some embodiments of this application, the materials of the pH detection unit include alizarin red and hexadecyltrimethylammonium bromide; the mass ratio of alizarin red to hexadecyltrimethylammonium bromide is (1-20):(1-35).

[0050] In some embodiments of this application, the detection patch further includes a first connector; the first connector is disposed on the side of the permeation layer near the isolation layer, and the permeation layer and the isolation layer are connected by the first connector.

[0051] In some embodiments of this application, the detection patch further includes a second connector. The second connector is disposed on the side of the colorimetric detection layer near the permeation layer, and the colorimetric detection layer and the permeation layer are connected by the second connector.

[0052] This application provides a detection patch, which includes a base layer and a colorimetric detection layer. The colorimetric detection layer is disposed on one side of the base layer in the thickness direction; the detection layer includes a carrier and a colorimetric detection part, which is disposed on the side of the carrier opposite to the base layer. Both the base layer and the colorimetric detection layer are paper base layers. By using paper base layers for both the base layer and the colorimetric detection layer, the paper base layer can be processed through simple cutting, folding, and lamination processes. This makes the base layer and the colorimetric detection layer easy to process and produce, reducing the complexity of the detection patch preparation.

[0053] Furthermore, the colorimetric detection layer indicates the presence or concentration changes of substances in sweat through color changes. The paper-based colorimetric detection layer can quickly absorb and fix chemical reagents, and the paper-based material also contributes to the uniformity of chemical reagent coating, thus improving the detection accuracy and consistency of the detection patch. Attached Figure Description

[0054] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this application and, together with the description, serve to explain the principles of this application.

[0055] Figure 1 A schematic diagram of the structure of the detection patch provided in the embodiments of this application. Figure 1 ;

[0056] Figure 2 A schematic diagram of the structure of the detection patch provided in the embodiments of this application. Figure 2 ;

[0057] Figure 3 The detection results of Examples 2-7 of the detection patch provided in this application;

[0058] Figure 4 The detection results of Examples 8-13 of the detection patch provided in this application;

[0059] Figure 5 The detection results of embodiments 14-19 of the detection patch provided in this application;

[0060] Figure 6 The detection results of Examples 20-25 of the detection patch provided in this application;

[0061] Figure 7 The detection results of embodiments 26-32 of the detection patch provided in this application;

[0062] Figure 8 The detection results of embodiments 33-38 of the detection patch provided in this application;

[0063] Figure 9 The detection results of embodiments 39-44 of the detection patch provided in this application;

[0064] Figure 10 The test results of Examples 45-50 of the test patch provided in this application.

[0065] Explanation of reference numerals in the attached figures:

[0066] 100: Grassroots level;

[0067] 200: Colorimetric detection layer;

[0068] 300: Permeable layer;

[0069] 400: Isolation layer;

[0070] 500: Transparent overlay.

[0071] The accompanying drawings illustrate specific embodiments of this application, which will be described in more detail below. These drawings and descriptions are not intended to limit the scope of the concept in any way, but rather to illustrate the concept of this application to those skilled in the art through reference to particular embodiments. Detailed Implementation

[0072] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numbers in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this application. Rather, they are merely examples of apparatuses and methods consistent with some aspects of this application as detailed in the appended claims.

[0073] Real-time monitoring of physiological indicators related to exercise and health helps athletes understand their metabolic status and exercise adaptation. Common physiological indicators include glucose, lactate, nitrogenous small molecules like urea, and pH. Glucose levels are related to an athlete's health and nutritional status; monitoring glucose levels can prevent fainting caused by hypoglycemia. When athletes engage in strenuous exercise, anaerobic metabolism leads to lactate buildup, causing muscle soreness; monitoring lactate levels can guide subsequent exercise. Urea nitrogenous small molecule levels can serve as an indicator of an athlete's metabolic rate and can also reflect kidney function. pH levels can indicate metabolic alkalosis.

[0074] Sweat, as a bodily fluid, contains various metabolic products and electrolytes, and its composition can reflect physiological indicators. Furthermore, sweat collection, as a typical non-invasive method, can facilitate the real-time, non-invasive detection of physiological indicators.

[0075] Taking electrochemical detection equipment as an example, commercially available electronic sweat detection products have the advantages of integrating multiple functions and continuously detecting sweat physiological indicators. However, these products are bulky and poorly portable. Their large size and weight make them uncomfortable to wear during exercise, potentially affecting the user's experience. Furthermore, they are expensive, complex in structure, and require cumbersome manufacturing processes. Priced around 1000 yuan, these products offer low cost-effectiveness for non-professional athletes and are not widely available. Moreover, these products incorporate multiple electronic data acquisition and processing units, requiring sophisticated manufacturing processes and making mass production difficult. They also require regular calibration, making maintenance cumbersome. The activity of enzymes in the detection module changes with environmental and temporal factors, and impurities accumulate over long-term use. Therefore, regular calibration and maintenance are necessary to ensure accurate results. Users must also manually set various parameters, reducing ease of use. Because users need to input personalized data before use, the user experience is poor, lacking convenience and speed.

[0076] Electrochemical sweat testing patches have market value due to their small size, light weight, high sensitivity, and accuracy. However, the manufacturing process of these patches based on electrochemical methods is relatively complex and costly, making them less consumer-friendly for athletes. Furthermore, the results of these sweat testing patches are often displayed on external devices rather than the patches themselves, requiring athletes to carry their phones to view the results, which is inconvenient.

[0077] All of the above indicates that electrochemical sweat detection methods are not suitable for the daily detection of sweat physiological indicators by athletes.

[0078] Further research by researchers revealed that colorimetric sweat detection is cost-effective and easy to use. In related technologies, colorimetric sweat detection patches made of fabrics and fibers can obtain physiological information through color comparison.

[0079] However, the fabrication of colorimetric detection patches for sweat on textiles and fibers is complex.

[0080] Therefore, this application provides a detection patch comprising a base layer and a colorimetric detection layer. The colorimetric detection layer is disposed on one side of the base layer in the thickness direction; the detection layer includes a carrier and a colorimetric detection part, the colorimetric detection part being disposed on the side of the carrier opposite to the base layer. Both the base layer and the colorimetric detection layer are paper base layers. By setting the base layer and the colorimetric detection layer as paper base layers, the paper base layer can be processed through simple cutting, folding, and lamination processes. This makes the base layer and the colorimetric detection layer easy to process and produce, reducing the complexity of the detection patch preparation.

[0081] Colorimetric detection layers indicate the presence or concentration changes of substances in sweat through color changes. The paper-based material of the colorimetric detection layer allows for rapid absorption and fixation of chemical reagents, and also contributes to the uniformity of chemical reagent coating, thereby improving the accuracy and consistency of the detection patch.

[0082] Furthermore, paper-based detection patches have the advantages of small size and high portability. Athletes do not feel the wearer's body during exercise, and it is unlikely to affect their physical sensations; paper-based detection patches are also low in cost and simple to manufacture.

[0083] The technical solution of this application and how the technical solution of this application solves the above-mentioned technical problems are described in detail below with specific embodiments. These specific embodiments can be combined with each other, and the same or similar concepts or processes may not be described again in some embodiments. The embodiments of this application will now be described with reference to the accompanying drawings.

[0084] Reference Figure 1 and Figure 2 As shown, this application embodiment provides a detection patch, including a base layer 100 and a colorimetric detection layer 200. The colorimetric detection layer 200 is disposed on one side of the base layer 100 in the thickness direction; the colorimetric detection layer 200 includes a carrier and a colorimetric detection part, the colorimetric detection part being disposed on the side of the carrier opposite to the base layer 100.

[0085] Both the base layer 100 and the colorimetric detection layer 200 are paper base layers.

[0086] For example, the base layer 100 provides physical support and structural integrity for the entire detection patch, maintaining the shape and stability of the detection patch.

[0087] Taking the detection patch for sweat as an example, the colorimetric detection layer 200 indicates the presence or concentration changes of substances in sweat through color changes. In this way, the detection patch can detect changes in sweat substances in real time, providing immediate physiological information feedback, and is suitable for athletes, patients, or people who need to monitor physiological indicators.

[0088] The substances include glucose, urea, lactic acid, and pH.

[0089] In addition, the detection patch analyzes sweat rather than bodily fluids such as blood, reducing invasiveness and discomfort for the user.

[0090] For example, by setting the base layer 100 and the colorimetric detection layer 200 as a paper base layer, the paper base layer can be processed by simple cutting, folding and lamination processes, which reduces the preparation complexity of the base layer 100 and the colorimetric detection layer 200, and reduces the preparation complexity of the detection patch.

[0091] Compared to fabric-based testing patches, fabrics and fibers may require more pretreatment steps to ensure uniform coating of the test reagent. In contrast, the paper-based colorimetric detection layer 200 can be prepared using simple coating or printing techniques. These processes are relatively simple and do not require complex equipment or multiple steps. Furthermore, fabrics and fibers may require more complex cutting and sewing processes to form the desired shape of the testing patch, while paper is easy to cut and shape, and can be quickly produced in the desired shape using simple processes such as die-cutting.

[0092] Among them, the test reagents refer to the test reagents used by the colorimetric detection unit to detect sweat substances and perform color development.

[0093] Furthermore, the paper substrate can be enhanced with chemical treatments or physical modifications to improve its specific functions, such as increasing hydrophilicity, hydrophobicity, or specific chemical reactivity, thereby meeting diverse needs. Depending on the specific testing requirements, the paper substrate can be customized with different thicknesses, densities, and surface properties.

[0094] Since both the base layer 100 and the colorimetric detection layer 200 are made of paper, the paper base layer is lightweight and soft, conforming well to the human skin and providing a comfortable wearing experience. At the same time, the paper base layer has good breathability, making it suitable for prolonged wear. Furthermore, the paper base layer is relatively environmentally friendly, being biodegradable or recyclable, reducing its impact on the environment.

[0095] Furthermore, the colorimetric detection layer 200 indicates the presence or concentration changes of substances in sweat through color changes. The paper-based colorimetric detection layer 200 can quickly absorb and fix the test reagents, and the paper-based colorimetric detection layer 200 helps to ensure the uniformity of the test reagent coating, thus improving the detection accuracy and consistency of the test patch.

[0096] Understandably, the detection patch can be used to detect sweat as well as other liquid samples.

[0097] As one feasible implementation, the base layer 100 includes release paper. During transportation and storage, the release paper protects the test patch from dust, moisture, and other environmental factors. The base layer 100 serves to shield against light and oxygen, extending shelf life. Release paper is typically low-cost, suitable for large-scale production, and provides a cost-effective solution.

[0098] As one feasible implementation, the carrier includes silicone paper. Silicone paper is inert to most chemicals and does not readily react with the test reagents in the colorimetric detection unit, thereby ensuring the accuracy and stability of the detection.

[0099] In addition, silicone paper is easy to cut, die-cut and process, making it suitable for mass production and customized needs.

[0100] As one feasible implementation, the colorimetric detection unit includes glass fiber filter paper. The glass fiber filter paper has excellent adsorption capacity, effectively absorbing sweat and test reagents, ensuring sufficient contact between sweat and the test reagents in the colorimetric detection unit, thereby improving the sensitivity and accuracy of the detection. Simultaneously, the glass fiber filter paper is breathable, which helps sweat to be quickly and evenly distributed in the colorimetric detection unit, improving the detection efficiency of the detection patch.

[0101] In addition, glass fiber materials are inert to most chemicals and do not readily react with test reagents, which helps maintain the stability and reliability of the test patch results.

[0102] For example, the colorimetric detection unit is fitted tightly into the small holes provided on the carrier by friction. The carrier has four small holes, each with a diameter of about 3 mm, symmetrically distributed in a two-upper-two-lower configuration, with a center-to-center distance of about 7 mm between adjacent holes.

[0103] As one feasible implementation, the detection patch also includes a permeation layer 300, which is disposed between the colorimetric detection layer 200 and the base layer 100. The permeation layer 300 acts as a buffer layer, preventing excessive sweat from directly contacting the colorimetric detection layer 200 and avoiding dilution or rinsing of the test reagents in the colorimetric detection layer 200, thus affecting the test results. Simultaneously, the permeation layer 300 can block impurities or particulate matter from entering the colorimetric detection layer 200, reducing interference from impurities on the test results.

[0104] The permeation layer 300 can effectively control the flow speed and direction of sweat or other liquids from the base layer 100 to the colorimetric detection layer 200, ensuring uniform distribution of sweat and thus improving the accuracy and consistency of the detection patch.

[0105] In addition, the penetration layer 300 can increase the overall structural strength of the detection patch and prevent damage caused by bending or stretching during use.

[0106] As one possible implementation, the detection patch also includes a second connector. The second connector is disposed on the side of the colorimetric detection layer 200 near the permeation layer 300, and the colorimetric detection layer 200 and the permeation layer 300 are connected by the second connector.

[0107] For example, the second connector can be medical waterproof double-sided tape. The width of the waterproof double-sided tape is 3 mm. The connector ensures tight adhesion between the periphery of the permeation layer 300 and the periphery of the carrier of the colorimetric detection layer 200.

[0108] As one feasible implementation, the permeation layer 300 is a paper base layer. As the permeation layer 300, the paper base layer has good liquid absorption, effectively controlling the flow and distribution of sweat or other liquids, ensuring that the liquid is evenly transferred to the colorimetric detection layer 200. At the same time, the paper base layer 300 is lightweight, not significantly increasing the weight of the detection patch, thus improving the portability and comfort of the detection patch.

[0109] As one feasible implementation, the permeation layer 300 includes cotton fiber filter paper. The cotton fiber filter paper has excellent liquid absorption properties, enabling it to quickly absorb and transfer sweat, ensuring that sweat can permeate evenly into the colorimetric detection unit. Simultaneously, the cotton fiber filter paper can effectively filter out larger particles and impurities, ensuring that only liquid samples enter the colorimetric detection unit, thus improving the detection accuracy of the detection patch.

[0110] Furthermore, cotton fiber filter paper is biocompatible and will not adversely affect the skin or samples, making it suitable for applications involving direct skin contact. The soft nature of cotton fibers makes it more comfortable when in contact with the skin, suitable for testing patches worn for extended periods.

[0111] Cotton fiber filter paper is renewable and biodegradable, meets environmental protection requirements, and reduces environmental impact.

[0112] As one possible implementation, the detection patch also includes a transparent overlay layer 500, which is disposed on the side of the colorimetric detection layer 200 opposite to the base layer 100.

[0113] For example, when the test patch is in storage, the transparent cover layer 500 can effectively protect the colorimetric test layer 200 from external physical damage, contamination and moisture, thus extending the service life of the test patch.

[0114] During the use of the testing patch, because the transparent overlay 500 is transparent, the overlay allows users to clearly observe the color change of the colorimetric detection layer 200, ensuring intuitive reading of the test results.

[0115] In addition, the transparent overlay 500 increases the overall structural strength of the detection patch, making it more resistant to wear and tear during use.

[0116] For example, the side of the transparent overlay 500 facing the colorimetric detection layer 200 is adhesive.

[0117] As one possible implementation, the transparent overlay 500 is made of materials including polyethylene terephthalate, polyethylene, and polypropylene.

[0118] Polyethylene terephthalate (PET) is waterproof, effectively preventing external moisture or liquids from penetrating the colorimetric detection layer 200, thus avoiding contamination of the detection patch during storage. Simultaneously, PET is chemically stable and will not react with the chemical reagents in the colorimetric detection layer 200, ensuring the accuracy of the test results.

[0119] In addition, polyethylene terephthalate (PET) is transparent, allowing users to clearly observe the color change of the colorimetric detection layer 200, making it easy to read the test results intuitively.

[0120] Polypropylene has a low density, which makes the testing patch lightweight, easy to carry, and easy to use. Polypropylene also has chemical stability.

[0121] Polyethylene is tough and can withstand a certain degree of physical deformation without damage. Its waterproof properties ensure the testing patch functions properly in humid environments. Polyethylene is resistant to chemicals, ensuring compatibility with the colorimetric testing layer 200.

[0122] As one feasible implementation, the detection patch also includes an isolation layer 400. The isolation layer 400 is disposed between the permeable layer 300 and the base layer 100.

[0123] The isolation layer 400 is provided with mounting holes, and the permeable layer 300 contacts the base layer 100 through the mounting holes.

[0124] For example, the isolation layer 400 provides double protection against sweat seeping back into the skin; at the same time, the isolation layer 400 helps to enhance the overall seal of the detection patch and prevent external environmental factors (such as moisture and dust) from interfering with the color detection layer 200.

[0125] The design of the isolation layer 400 simplifies the assembly process of the detection patch, making the installation of the colorimetric detection layer 200 and the penetration layer 300 more convenient and efficient.

[0126] The length of the mounting hole ranges from 1 to 2 cm, and the width ranges from 0.5 to 1.5 cm.

[0127] As one possible implementation, the detection patch also includes a first connector; the first connector is disposed on the side of the permeation layer 300 near the isolation layer 400, and the permeation layer 300 and the isolation layer 400 are connected by the first connector.

[0128] For example, the first connector can be medical-grade waterproof double-sided tape. The width of the waterproof double-sided tape is 3mm. The edges of the mounting hole and the edges of the permeable layer 300 are tightly adhered by the medical-grade waterproof double-sided tape.

[0129] As one feasible implementation method, the isolation layer 400 is a paper base layer.

[0130] For example, the paper base layer can be processed through simple cutting, folding and lamination processes, which reduces the complexity of the preparation of the isolation layer 400 and the complexity of the preparation of the detection patch.

[0131] As one feasible implementation, the insulating layer 400 includes silicone paper. The silicone paper material prevents sweat from seeping back onto the skin, providing dual protection on top of the water-locking effect of the glass fiber filter paper material.

[0132] As one feasible implementation, the number of colorimetric detection units is multiple.

[0133] Multiple colorimetric detection units allow for the simultaneous detection of various substances. This enables the detection patch to provide more comprehensive analytical results, suitable for complex physiological or environmental assays. Furthermore, the substances detected by multiple colorimetric detection units can be the same, allowing for repeated detection of the same substance, cross-validation of results, improved accuracy and reliability, and reduced errors.

[0134] For example, the preparation process of the colorimetric detection unit is as follows: first, liquid detection reagent is dropped onto glass fiber filter paper, and after the detection reagent dries, the glass fiber filter paper carrying the detection reagent forms the colorimetric detection unit.

[0135] In some embodiments, multiple colorimetric detection units are arranged at intervals along the extension plane of the carrier. This interval arrangement optimizes the space utilization of the carrier, ensuring that each colorimetric detection unit has sufficient space to react and display results, thus avoiding mutual interference. By arranging multiple colorimetric detection units at intervals, cross-interference between different colorimetric detection units can be effectively reduced, ensuring that the results of each colorimetric detection unit are independent and accurate.

[0136] In other embodiments, the multiple colorimetric detection units are made of different materials and are used to detect different substances.

[0137] By using colorimetric detection units made of different materials, the detection patches can simultaneously detect multiple different types of substances, such as pH values, specific ions, enzyme activity, and metabolites, adapting to a wider range of application needs. Thus, based on specific customer application requirements, different materials can be selected and combined to customize the detection patch design to meet specific detection requirements.

[0138] The colorimetric detection section is made of a test reagent used to detect sweat and produce color.

[0139] For example, during the use of the detection patch, the base layer 100 of the detection patch is peeled off, and the detection patch is attached to clean skin, with the transparent cover layer 500 facing upwards. After continuous exercise for a period of time, sweat flows through the permeation layer 300 to the colorimetric detection layer 200. Different colorimetric detection sections in the colorimetric detection layer 200 monitor the sweat. The sweat develops color in the colorimetric detection sections, and the degree of color development is identified and analyzed by the naked eye and compared with a color chart to obtain the index values ​​of glucose, lactic acid, urea, and pH in the sweat.

[0140] For example, the thickness of the base layer 100 ranges from 0.05mm to 0.15mm.

[0141] In some embodiments, the thickness of the base layer 100 is 0.1 mm.

[0142] As one feasible implementation, the colorimetric detection unit has a second pore, the pore diameter D2 of which satisfies: 0.5μm≤D2≤1.5μm.

[0143] For example, when the pore size of the second aperture is between 0.5μm and 1.5μm, the colorimetric detection unit can lock sweat and the material of the colorimetric detection unit, effectively keeping the material of the colorimetric detection unit inside the colorimetric detection unit and preventing it from being lost or diffused to other areas, thereby ensuring the accuracy and sensitivity of the detection.

[0144] Optionally, 0.5μm ≤ D2 ≤ 1.0μm, or 1.0μm ≤ D2 ≤ 1.5μm, or 0.8μm ≤ D2 ≤ 1.2μm. Alternatively, D2 can be 0.5μm, 0.6μm, 0.7μm, 0.8μm, 0.9μm, 1.0μm, 1.1μm, 1.2μm, 1.3μm, 1.4μm, or 1.5μm.

[0145] As one feasible implementation, the thickness of the colorimetric detection unit is P2, where P2 satisfies: 1.5mm ≤ P2 ≤ 2.5mm. This thickness range provides sufficient volume to accommodate an appropriate amount of material and sweat, ensuring that the chemical reaction can proceed fully and improving detection sensitivity and accuracy.

[0146] Optionally, 1.5mm ≤ P2 ≤ 2.0mm. Or, 2.0mm ≤ P2 ≤ 2.5mm. Or, 1.8mm ≤ P2 ≤ 2.2mm. Alternatively, P2 can be 1.6mm, 1.7mm, 1.8mm, 1.9mm, 2.0mm, 2.1mm, 2.2mm, 2.3mm, or 2.4mm.

[0147] As one feasible implementation, the permeable layer 300 has a first pore, the pore size D1 of the first pore satisfying: 8μm≤D1≤15μm.

[0148] For example, the first pore size range of 8 μm to 15 μm can effectively control the permeation rate of sweat, ensuring that sweat can be uniformly and appropriately delivered to the colorimetric detection layer 200.

[0149] Optionally, 8μm≤D1≤11μm. Or, 11μm≤D1≤15μm. Or, 10μm≤D1≤12μm. Alternatively, D1 can be 8μm, 9μm, 10μm, 11μm, 12μm, 13μm, or 14μm.

[0150] As one feasible implementation, the thickness of the permeation layer 300 is P1, where P1 satisfies: 0.15 ≤ P1 ≤ 0.25 mm. When the thickness of the permeation layer 300 is in the range of 0.15-0.25 mm, the permeation layer 300 allows body sweat to pass through the permeation layer 300 at an appropriate speed, ensuring that the sweat reaches the colorimetric detection layer 200 evenly, thereby improving the accuracy and consistency of the detection.

[0151] Optionally, 0.15mm ≤ P1 ≤ 0.20mm. Or, 0.20mm ≤ P1 ≤ 0.25mm. Or, 0.18mm ≤ P1 ≤ 0.23mm. Alternatively, P1 can be 0.15mm, 0.16mm, 0.17mm, 0.18mm, 0.19mm, 0.20mm, 0.21mm, 0.22mm, 0.23mm, or 0.24mm.

[0152] As one feasible implementation, the thickness of the isolation layer 400 is P3, where P3 satisfies: 0.05mm ≤ P3 ≤ 0.15mm. This thickness range is sufficient to provide effective isolation. A mounting hole is formed in the central portion of the isolation layer 400, and the periphery of the isolation layer 400 is tightly adhered to the permeable layer 300 to prevent sweat from flowing around the detection patch.

[0153] Optionally, 0.05mm ≤ P3 ≤ 0.10mm. Or, 0.10mm ≤ P3 ≤ 0.15mm. Or, 0.08mm ≤ P3 ≤ 0.12mm. Alternatively, P3 can be 0.06mm, 0.07mm, 0.08mm, 0.09mm, 0.10mm, 0.11mm, 0.12mm, 0.13mm, or 0.14mm.

[0154] For example, the thickness of the transparent overlay 500 is 0.2 mm.

[0155] As one feasible implementation, the colorimetric detection unit includes a glucose detection unit, a lactic acid detection unit, a urea detection unit, and a pH detection unit.

[0156] The glucose detection unit, lactic acid detection unit, urea detection unit, and pH detection unit are arranged in an array on the carrier.

[0157] For example, by integrating multiple colorimetric detection units onto a single carrier, multiple substances, such as glucose, lactic acid, urea, and pH, can be detected simultaneously. This multi-substance detection capability improves detection efficiency and data comprehensiveness.

[0158] The array-like distribution allows multiple colorimetric detection units to be compactly arranged, saving space and making it suitable for portable and miniaturized devices. Arranging different detection parts in an array facilitates visual or instrument reading and comparison of the detection results of various parameters.

[0159] As one feasible implementation, the materials of the glucose detection unit include a glucose catalyst, a peroxide catalyst, a colorimetric reagent, and a pH reagent.

[0160] Glucose catalysts catalyze the oxidation of glucose to produce gluconic acid and hydrogen peroxide.

[0161] Peroxide catalysts use the generated hydrogen peroxide to oxidize the colorimetric reagent, thereby producing a detectable signal (usually a color change).

[0162] pH reagents are used to maintain the optimal pH value for the reaction, ensuring enzyme activity and reaction accuracy. pH buffers can help stabilize the reaction environment.

[0163] As one feasible implementation, the glucose catalyst includes glucose oxidase.

[0164] For example, glucose oxidase (GOx) is an enzyme catalyst specifically designed to catalyze the oxidation of glucose to gluconic acid, while simultaneously producing hydrogen peroxide. It is a commonly used biocatalyst in glucose detection due to its high specificity.

[0165] As one feasible implementation, the peroxide catalyst includes horseradish peroxidase.

[0166] Horseradish peroxidase (HRP) is an enzyme that catalyzes the decomposition of hydrogen peroxide.

[0167] As one possible implementation, the color developer includes 3,3'-dimethoxybenzidine.

[0168] 3,3'-Dimethoxybenzidine is a chromogenic agent that is oxidized during enzymatic reactions, producing color changes.

[0169] In the detection process, HRP reacts with hydrogen peroxide, and 3,3'-dimethoxybenzidine produces a color change, which facilitates visual detection.

[0170] As one feasible implementation, the pH reagent includes a citrate-disodium hydrogen phosphate buffer solution.

[0171] Citrate-disodium hydrogen phosphate buffer provides a stable pH environment, ensuring optimal enzyme activity. Maintaining the pH of the reaction system ensures efficient enzyme reactions and improves detection accuracy.

[0172] As one feasible implementation, the glucose detection unit comprises glucose oxidase, horseradish peroxidase, and 3,3'-dimethoxybenzidine; the mass ratio of glucose oxidase, horseradish peroxidase, and 3,3'-dimethoxybenzidine is (4-30):(1-20):(1-8). Within this mass ratio range, glucose oxidase (GOx) catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. An appropriate GOx concentration ensures rapid glucose conversion and improves detection sensitivity; horseradish peroxidase (HRP) effectively catalyzes the decomposition of hydrogen peroxide and reacts with 3,3'-dimethoxybenzidine to produce a noticeable color change. An appropriate amount of HRP ensures a rapid and efficient colorimetric reaction; 3,3'-dimethoxybenzidine provides good color contrast within this ratio range, making the detection results easier to observe and analyze visually. An appropriate concentration of the chromogenic agent ensures the clarity and contrast of the color change.

[0173] The glucose detection unit is prepared from a glucose detection solution. This solution includes an aqueous solution of glucose oxidase, an aqueous solution of horseradish peroxidase, an aqueous solution of 3,3'-dimethoxybenzidine, and a citrate-disodium hydrogen phosphate buffer solution.

[0174] Add the above glucose detection solution to glass fiber filter paper. Dry at room temperature to form the glucose detection section.

[0175] The glucose detection unit provided in this application is superior to traditional glucose detection formulas. Traditional glucose detection formulas show little color difference in the detection results after detecting different glucose concentrations of the detection solution, making them difficult to distinguish visually.

[0176] As one feasible implementation, the materials of the lactic acid detection unit include a lactic acid catalyst, a peroxide catalyst, a colorimetric reagent, and a pH reagent.

[0177] Lactic acid catalysts can catalyze the oxidation of lactic acid to produce pyruvate and hydrogen peroxide.

[0178] As a peroxide catalyst, it utilizes the hydrogen peroxide generated in the reaction to oxidize the colorimetric reagent, resulting in a color change, thereby achieving detection.

[0179] The color developer reacts with hydrogen peroxide under the action of a peroxide catalyst to produce a colored product.

[0180] pH reagents are used to maintain the optimal pH value for the reaction to ensure its stability.

[0181] As one feasible implementation, the lactic acid catalyst includes lactate oxidase.

[0182] Lactate oxidase is a key biocatalyst that catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide. This reaction is fundamental to lactate detection. Lactate oxidase exhibits high specificity and sensitivity, enabling accurate detection of lactate concentrations in sweat.

[0183] As one feasible implementation, the peroxide catalyst includes peroxidase.

[0184] Peroxidase catalyzes the reaction of hydrogen peroxide with chromogenic agents (such as 4-aminoantipyrine and phenolic compounds) to produce measurable color changes.

[0185] As one possible implementation, the colorimetric agent includes 4-aminoantipyrine.

[0186] 4-Aminoantipyrine, as a colorimetric agent, reacts with peroxidase to produce a colored compound, and the color intensity is directly proportional to the lactic acid concentration.

[0187] As one feasible implementation, the pH reagent includes a citrate-disodium hydrogen phosphate buffer solution.

[0188] Citrate-disodium hydrogen phosphate buffer provides a stable pH environment, ensuring that enzymes and chemical reactions proceed under optimal conditions.

[0189] As one feasible implementation, the materials of the lactate detection unit include lactate oxidase, peroxidase, and 4-aminoantipyrine; the mass ratio of lactate oxidase, peroxidase, and 4-aminoantipyrine is (1-20):(2-40):(7-30). Within this mass ratio range, lactate oxidase catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide. An appropriate concentration of lactate oxidase ensures rapid conversion of lactate, improving the sensitivity and specificity of detection; peroxidase effectively catalyzes the reaction of hydrogen peroxide with 4-aminoantipyrine, producing a noticeable color change. An appropriate amount of peroxidase ensures the rapid and efficient execution of the colorimetric reaction; 4-aminoantipyrine provides good color contrast, making the detection results easier to observe and analyze visually. An appropriate concentration of the chromogenic agent ensures the clarity and contrast of the color change, with color intensity proportional to the lactate concentration, facilitating quantitative analysis.

[0190] The lactate detection unit is prepared using a lactate detection solution. This solution includes an aqueous solution of lactate oxidase, an aqueous solution of peroxidase, an aqueous solution of 4-aminoantipyrine, and a citrate-disodium hydrogen phosphate buffer solution.

[0191] The above lactic acid detection solution was added to glass fiber filter paper. It was then air-dried at room temperature to form the lactic acid detection section.

[0192] The lactic acid detection unit provided in this application is superior to traditional lactic acid detection formulas. Traditional lactic acid detection formulas show little difference in color after detecting different lactic acid concentrations in the detection solution, making it difficult to distinguish with the naked eye.

[0193] As one feasible implementation, the materials of the urea detection unit include p-dimethylaminobenzaldehyde, sulfate, and pH reagent.

[0194] p-Dimethylaminobenzaldehyde is a colorimetric reagent commonly used to detect urea. In the presence of sulfuric acid or sulfate, urea reacts with p-dimethylaminobenzaldehyde to form a yellow compound; the intensity of the color is directly proportional to the urea concentration. This color change can be quantitatively determined using a colorimetric method.

[0195] Sulfates are used to promote the reaction of p-dimethylaminobenzaldehyde with urea.

[0196] pH reagents are used to maintain the optimal pH value for the reaction, ensuring its stability and accuracy. pH reagents can be buffer solutions, such as citrate-disodium hydrogen phosphate buffer, to provide a suitable acidic environment for the reaction.

[0197] As one possible implementation, sulfates include potassium hydrogen sulfate.

[0198] Potassium bisulfate (KHSO4) is used to provide sulfate ions, which promote the reaction between urea and p-dimethylaminobenzaldehyde. Sulfate ions are crucial for ensuring the successful progress of the colorimetric reaction.

[0199] As one feasible implementation, the pH reagent includes a citrate-disodium hydrogen phosphate buffer solution.

[0200] Citrate-disodium hydrogen phosphate buffer provides a stable pH environment.

[0201] In one feasible implementation, the urea detection unit is made of p-dimethylaminobenzaldehyde and potassium bisulfate; the mass ratio of p-dimethylaminobenzaldehyde to potassium bisulfate is (3-20):(1-40). Within this mass ratio range, p-dimethylaminobenzaldehyde can effectively react with urea to form a yellow compound. Appropriate amounts of p-dimethylaminobenzaldehyde ensure clarity and contrast of the color change, making the detection results easier to observe and analyze. Potassium bisulfate provides the necessary sulfate ions to promote the reaction between urea and p-dimethylaminobenzaldehyde. Sufficient sulfate ions ensure the smooth progress of the colorimetric reaction, improving the sensitivity and accuracy of the detection.

[0202] The urea testing section is prepared using urea test solution. The urea test solution includes p-dimethylaminobenzaldehyde alcohol solution, potassium hydrogen sulfate aqueous solution, and citrate-disodium hydrogen phosphate buffer.

[0203] The above urea detection solution is added to glass fiber filter paper. It is then dried at room temperature to form the urea detection section.

[0204] As one feasible implementation, the materials of the pH detection unit include pH indicator and stabilizer.

[0205] pH indicators can display different color changes at different pH values. This makes them suitable for a variety of pH detection applications.

[0206] Stabilizers are used to stabilize chemical reaction systems.

[0207] As one feasible implementation, the pH indicator includes alizarin red.

[0208] Alizarin Red is a pH indicator whose color changes with the ambient pH. It is commonly used to detect pH changes within acidic or neutral ranges. Alizarin Red exhibits a distinct color change, facilitating visual observation and quantitative analysis.

[0209] As one feasible implementation, the stabilizer includes hexadecyltrimethylammonium bromide.

[0210] Hexadecyltrimethylammonium bromide (CTAB) is a cationic surfactant that can improve the uniformity and sensitivity of detection membranes. It can interact with alizarin red to enhance the contrast of color changes.

[0211] The urea detection unit provided in this embodiment is superior to the traditional urea detection formula, which shows little difference in color after detecting different urea concentrations in the detection solution, making it difficult to distinguish with the naked eye.

[0212] As one feasible implementation, the pH detection unit is made of alizarin red and hexadecyltrimethylammonium bromide; the mass ratio of alizarin red to hexadecyltrimethylammonium bromide is (1-20):(1-35). Within this mass ratio range, alizarin red can effectively display color changes at different pH values. An appropriate concentration of alizarin red ensures the clarity and contrast of the color changes, making the pH detection results easier to observe and analyze visually. CTAB can effectively improve the uniformity and sensitivity of the detection membrane. Its interaction with alizarin red enhances the contrast of the color changes and prevents the indicator from degrading or precipitating during storage or use.

[0213] The pH detection unit provided in this application is superior to traditional pH detection formulas, which show little color difference in the detection results after testing different pH solutions, making it difficult to distinguish with the naked eye.

[0214] The pH detection unit is prepared using a pH detection solution. The pH detection solution includes an aqueous solution of alizarin red and an aqueous solution of hexadecyltrimethylammonium bromide.

[0215] Add the pH detection solution to glass fiber filter paper. Dry at room temperature to form the pH detection section.

[0216] In some embodiments, the length of the permeable layer 300 ranges from 2-3 cm, and the width of the permeable layer 300 ranges from 1-3 cm. The length of the isolation layer 400 ranges from 3-4 cm, and the width of the isolation layer 400 ranges from 2-4 cm. The length of the transparent overlay layer 500 ranges from 5-7 cm, and the width of the transparent overlay layer 500 ranges from 4-6 cm. The length of the base layer 100 ranges from 4-7 cm, and the width of the base layer 100 ranges from 4-6 cm.

[0217] In other embodiments, the permeable layer 300 has a length of 2.5 cm and a width of 2 cm. The isolation layer 400 has a length of 3.5 cm and a width of 3 cm. The transparent overlay layer 500 has a length of 6 cm and a width of 5 cm. The base layer 100 has a length of 6 cm and a width of 5 cm.

[0218] The base layer 100 of the detection patch is peeled off and attached to the volunteer's chest, with the transparent cover layer 500 facing upwards. After the volunteer continuously engaged in activities such as (jumping rope for 20 minutes, doing aerobics for 20 minutes, playing tennis for 10 minutes, and running for 15 minutes), the colorimetric detection layer 200 of the detection patch was soaked with sweat. The colorimetric detection part developed color in the presence of sweat. By visually identifying and analyzing the degree of color development and comparing it with a color chart, the index values ​​of glucose, lactic acid, urea, and pH in the sweat can be obtained. Among them: a decrease in glucose index indicates a reduction in glucose concentration, and the athlete needs to replenish carbohydrates in time; an increase in lactic acid index indicates an increase in muscle fatigue, and the athlete needs to rest and recuperate in time; an increase in urea index indicates that kidney function is temporarily suppressed during exercise and prompts timely urination, while a decrease in the index indicates rapid sweat loss; the pH index can warn of metabolic alkalosis.

[0219] Example

[0220] Example 1

[0221] Glass fiber filter paper, silicone paper, and release paper are cut and joined together to form a test strip.

[0222] Example 2: A glucose detection solution was dropped onto glass fiber filter paper material with a diameter of 3 mm, and after drying, a glucose detection section was formed; wherein, the glucose detection solution included glucose oxidase aqueous solution, horseradish peroxidase aqueous solution, 3,3'-dimethoxybenzidine aqueous solution, and citrate-disodium hydrogen phosphate buffer; the pore size of the glass fiber filter paper material was about 1 micrometer and the thickness was about 2 mm.

[0223] The glucose test solution contains 4 mg / mL -1 1 μL glucose oxidase aqueous solution, 2 mg mL -1 2 μL horseradish peroxidase aqueous solution, 0.4 mg / mL -1 4 μL of 3,3'-dimethoxybenzidine aqueous solution, 1 mol L -1 3 μL citrate-disodium hydrogen phosphate buffer.

[0224] Add the test solution to the glucose detection unit. The glucose concentration in the test solution is 0.1 mM.

[0225] The difference between Examples 3 and 4 and Example 2 is that the mass of solutes in the glucose oxidase aqueous solution, horseradish peroxidase aqueous solution, 3,3'-dimethoxybenzidine aqueous solution, and citrate-disodium hydrogen phosphate buffer solution are different in Examples 3 and 4 compared to Example 2.

[0226] In Example 3, the glucose detection solution contained 1 mg / mL -11 μL glucose oxidase aqueous solution, 10 mg mL -1 5 μL horseradish peroxidase aqueous solution, 2 mg mL -1 2 μL of 3,3'-dimethoxybenzidine aqueous solution, 1 mol L -1 2 μL citrate-disodium hydrogen phosphate buffer.

[0227] In Example 4, the glucose detection solution contained 2 mg / mL -1 1 μL glucose oxidase aqueous solution, 1 mg / mL -1 1 μL horseradish peroxidase aqueous solution, 1 mg mL -1 9 μL of 3,3'-dimethoxybenzidine aqueous solution, 1 mol L -1 1 μL citrate-disodium hydrogen phosphate buffer.

[0228] Example 5: A glucose detection solution was dropped onto glass fiber filter paper material with a diameter of 3 mm, and after drying, a glucose detection section was formed; wherein, the glucose detection solution included glucose oxidase aqueous solution, horseradish peroxidase aqueous solution, 3,3'-dimethoxybenzidine aqueous solution, and citrate-disodium hydrogen phosphate buffer; the pore size of the glass fiber filter paper material was about 1 micrometer and the thickness was about 2 mm.

[0229] The glucose test solution contains 4 mg / mL -1 1 μL glucose oxidase aqueous solution, 2 mg mL -1 2 μL horseradish peroxidase aqueous solution, 0.4 mg / mL -1 4 μL of 3,3'-dimethoxybenzidine aqueous solution, 1 mol L -1 3 μL citrate-disodium hydrogen phosphate buffer.

[0230] Add the test solution to the glucose detection unit. The glucose concentration in the test solution is 0.3 mM.

[0231] The difference between Examples 6 and 7 and Example 5 is that the mass of the solutes in the glucose oxidase aqueous solution, horseradish peroxidase aqueous solution, 3,3'-dimethoxybenzidine aqueous solution, and citrate-disodium hydrogen phosphate buffer solution are different in Examples 6 and 7 compared to Example 5.

[0232] In Example 6, the glucose detection solution contained 1 mg / mL -1 1 μL glucose oxidase aqueous solution, 10 mg mL -1 5 μL horseradish peroxidase aqueous solution, 2 mg mL -1 2 μL of 3,3'-dimethoxybenzidine aqueous solution, 1 mol L -1 2 μL citrate-disodium hydrogen phosphate buffer.

[0233] In Example 7, the glucose detection solution contained 2 mg / mL -1 1 μL glucose oxidase aqueous solution, 1 mg / mL -1 1 μL horseradish peroxidase aqueous solution, 1 mg mL -1 9 μL of 3,3'-dimethoxybenzidine aqueous solution, 1 mol L -1 1 μL citrate-disodium hydrogen phosphate buffer.

[0234] Example 8

[0235] Lactic acid detection solution was dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a lactic acid detection section was formed. The lactic acid detection solution contained 0.5 mg / mL of the solution. -1 4 μL lactate oxidase aqueous solution, 10 mg mL -1 1 μL peroxidase aqueous solution, 5 mg mL -1 2 μL 4-aminoantipyrine aqueous solution, 20 mmol L -1 3 μL of citrate-disodium hydrogen phosphate buffer; the glass fiber filter paper material has a pore size of approximately 1 micrometer and a thickness of approximately 2 millimeters.

[0236] Add the test solution to the lactic acid detection unit. The lactic acid content in the test solution is 10 mM.

[0237] The difference between Examples 9 and 10 and Example 8 is that the mass of solute in the lactate oxidase aqueous solution, peroxidase aqueous solution, 4-aminoantipyrine aqueous solution, and citrate-disodium hydrogen phosphate buffer solution in Examples 9 and 10 is different from that in Example 8.

[0238] In Example 9, the lactate detection solution contained 0.05 mg / mL -1 2 μL lactate oxidase aqueous solution, 2 mg / mL -1 1 μL peroxidase aqueous solution, 4 mg mL -1 8 μL of 4-aminoantipyrine aqueous solution, 20 mmol / L -1 1 μL citrate-disodium hydrogen phosphate buffer.

[0239] In Example 10, the lactate detection solution contained 0.1 mg / mL -1 Lactate oxidase aqueous solution 1 μL, 0.5 mg mL -1 1 μL peroxidase aqueous solution, 1 mg mL -1 10 μL 4-aminoantipyrine aqueous solution, 20 mmol L -1 1 μL citrate-disodium hydrogen phosphate buffer.

[0240] Example 11

[0241] Lactic acid detection solution was dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a lactic acid detection section was formed. The lactic acid detection solution contained 0.5 mg / mL of the solution. -1 4 μL lactate oxidase aqueous solution, 10 mg mL -1 1 μL peroxidase aqueous solution, 5 mg mL -1 2 μL 4-aminoantipyrine aqueous solution, 20 mmol L -1 3 μL of citrate-disodium hydrogen phosphate buffer; the glass fiber filter paper material has a pore size of approximately 1 micrometer and a thickness of approximately 2 millimeters.

[0242] Add the test solution to the lactic acid detection unit. The lactic acid concentration in the test solution is 50 mM.

[0243] The difference between Examples 12 and 13 and Example 11 is that the mass of solutes in the lactate oxidase aqueous solution, peroxidase aqueous solution, 4-aminoantipyrine aqueous solution, and citrate-disodium hydrogen phosphate buffer in Examples 12 and 13 is different from that in Example 11.

[0244] In Example 12, the lactate detection solution contained 0.05 mg / mL -1 2 μL lactate oxidase aqueous solution, 2 mg / mL -1 1 μL peroxidase aqueous solution, 4 mg mL -1 8 μL of 4-aminoantipyrine aqueous solution, 20 mmol / L -1 1 μL citrate-disodium hydrogen phosphate buffer.

[0245] In Example 13, the lactate detection solution contained 0.1 mg / mL -1 1 μL lactate oxidase aqueous solution, 0.5 mg / mL -1 1 μL peroxidase aqueous solution, 1 mg mL -1 10 μL 4-aminoantipyrine aqueous solution, 20 mmol L -1 1 μL citrate-disodium hydrogen phosphate buffer.

[0246] Example 14

[0247] Urea detection solution was dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a urea detection section was formed. The urea detection solution contained 25 mg / mL of urea solution. -1 4 μL p-dimethylaminobenzaldehyde alcohol solution, 60 mg mL -1 3 μL potassium hydrogen sulfate aqueous solution, 20 mmol / L -13 μL citrate-disodium hydrogen phosphate buffer. The urea concentration in the test solution is 5 mM.

[0248] The difference between Examples 15 and 16 and Example 14 is that the mass of solutes in the p-dimethylaminobenzaldehyde alcohol solution, potassium hydrogen sulfate aqueous solution, and citrate-disodium hydrogen phosphate buffer solution in Examples 15 and 16 is different from that in Example 14.

[0249] In Example 15, the urea detection solution contained 2 mg / mL -1 2 μL p-dimethylaminobenzaldehyde alcohol solution, 10 mg mL -1 6 μL potassium hydrogen sulfate aqueous solution, 20 mmol / mL -1 2 μL citrate-disodium hydrogen phosphate buffer.

[0250] In Example 16, the urea detection solution contains 10 mg / mL -1 7 μL p-dimethylaminobenzaldehyde alcohol solution, 6 mg mL -1 0.5 μL potassium hydrogen sulfate aqueous solution, 20 mmol / L -1 2 μL citrate-disodium hydrogen phosphate buffer.

[0251] Example 17

[0252] Urea detection solution was dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a urea detection section was formed. The urea detection solution contained 25 mg / mL of urea solution. -1 4 μL p-dimethylaminobenzaldehyde alcohol solution, 60 mg mL -1 3 μL potassium hydrogen sulfate aqueous solution, 20 mmol / L -1 3 μL citrate-disodium hydrogen phosphate buffer. The urea concentration in the test solution is 40 mM.

[0253] The difference between Examples 18 and 19 and Example 17 is that the mass of solutes in the p-dimethylaminobenzaldehyde alcohol solution, potassium hydrogen sulfate aqueous solution, and citrate-disodium hydrogen phosphate buffer solution in Examples 18 and 19 is different from that in Example 17.

[0254] In Example 18, the urea detection solution contained 2 mg / mL -1 2 μL p-dimethylaminobenzaldehyde alcohol solution, 10 mg mL -1 6 μL potassium hydrogen sulfate aqueous solution, 20 mmol / mL -1 2 μL citrate-disodium hydrogen phosphate buffer.

[0255] In Example 19, the urea detection solution contains 10 mg / mL -17 μL p-dimethylaminobenzaldehyde alcohol solution, 6 mg mL -1 0.5 μL potassium hydrogen sulfate aqueous solution, 20 mmol / L -1 2 μL citrate-disodium hydrogen phosphate buffer.

[0256] Example 20

[0257] A pH detection solution was dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a pH detection section was formed. The pH detection solution contained 5 mg / mL of [unspecified substance]. -1 2 μL alizarin red aqueous solution, 5 mg mL -1 8 μL of hexadecyltrimethylammonium bromide aqueous solution. The glass fiber filter paper material has a pore size of approximately 1 micrometer and a thickness of approximately 2 millimeters.

[0258] The solution to be tested is added to the pH detection unit. The pH value of the solution to be tested is 4.

[0259] The difference between Examples 21 and 22 and Example 20 is that the mass of the solutes in the alizarin red aqueous solution and the hexadecyltrimethylammonium bromide aqueous solution in Examples 21 and 22 are different from those in Example 20.

[0260] In Example 21, the pH detection solution contained 1 mg / mL -1 2 μL alizarin red aqueous solution, 10 mg mL -1 8 μL of hexadecyltrimethylammonium bromide aqueous solution.

[0261] In Example 22, the pH detection solution contained 20 mg / mL -1 7 μL alizarin red aqueous solution, 2 mg mL -1 2 μL of hexadecyltrimethylammonium bromide aqueous solution.

[0262] Example 23

[0263] A pH detection solution was dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a pH detection section was formed. The pH detection solution contained 5 mg / mL of [unspecified substance]. -1 2 μL alizarin red aqueous solution, 5 mg mL -1 8 μL of hexadecyltrimethylammonium bromide aqueous solution. The glass fiber filter paper material has a pore size of approximately 1 micrometer and a thickness of approximately 2 millimeters.

[0264] The solution to be tested is added to the pH detection unit. The pH value of the solution to be tested is 8.

[0265] The difference between Examples 24 and 25 and Example 23 is that the mass of solutes in the alizarin red aqueous solution and the hexadecyltrimethylammonium bromide aqueous solution in Examples 24 and 25 is different from that in Example 23.

[0266] In Example 24, the pH detection solution contained 1 mg / mL -1 2 μL alizarin red aqueous solution, 10 mg mL -1 8 μL of hexadecyltrimethylammonium bromide aqueous solution.

[0267] In Example 25, the pH detection solution contained 20 mg / mL -1 7 μL alizarin red aqueous solution, 2 mg mL -1 2 μL of hexadecyltrimethylammonium bromide aqueous solution.

[0268] Example 26

[0269] Glucose detection solution is dropped onto glass fiber filter paper material with a diameter of 3 mm, and after drying, a glucose detection section is formed. The glucose detection solution includes glucose oxidase aqueous solution, horseradish peroxidase aqueous solution, 3,3'-dimethoxybenzidine aqueous solution, and citrate-disodium hydrogen phosphate buffer. The pore size of the glass fiber filter paper material is about 1 micrometer and the thickness is about 2 mm.

[0270] The glucose test solution contains 4 mg / mL -1 1 μL glucose oxidase aqueous solution, 2 mg mL -1 2 μL horseradish peroxidase aqueous solution, 0.4 mg / mL -1 4 μL of 3,3'-dimethoxybenzidine aqueous solution, 1 mol L -1 3 μL citrate-disodium hydrogen phosphate buffer.

[0271] Add the test solution to the glucose detection unit. The glucose content in the test solution is 0.

[0272] The difference between Examples 27-32 and Example 26 is that the glucose concentration in the test solution is different.

[0273] In Example 27, the concentration of glucose in the test solution was 0.05 mM.

[0274] In Example 28, the concentration of glucose in the test solution was 0.1 mM.

[0275] In Example 29, the concentration of glucose in the test solution was 0.15 mM.

[0276] In Example 30, the concentration of glucose in the test solution was 0.2 mM.

[0277] In Example 31, the concentration of glucose in the test solution was 0.25 mM.

[0278] In Example 32, the concentration of glucose in the test solution was 0.3 mM.

[0279] Example 33

[0280] Lactic acid detection solution is dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a lactic acid detection section is formed. The lactic acid detection solution includes an aqueous solution of lactate oxidase, an aqueous solution of peroxidase, an aqueous solution of 4-aminoantipyrine, and a citrate-disodium hydrogen phosphate buffer solution. The glass fiber filter paper has a pore size of approximately 1 micrometer and a thickness of approximately 2 mm.

[0281] The lactate test solution contains 0.5 mg / mL -1 4 μL lactate oxidase aqueous solution, 10 mg mL -1 1 μL peroxidase aqueous solution, 5 mg mL -1 2 μL 4-aminoantipyrine aqueous solution, 20 mmol L -1 3 μL citrate-disodium hydrogen phosphate buffer.

[0282] Add the test solution to the lactic acid detection unit. The lactic acid content in the test solution is 0.

[0283] The difference between Examples 34 to 38 and Example 33 is that the concentration of lactic acid in the test solution is different.

[0284] In Example 34, the concentration of lactic acid in the test solution was 10 mM.

[0285] In Example 35, the concentration of lactic acid in the test solution was 20 mM.

[0286] In Example 36, the concentration of lactic acid in the test solution was 30 mM.

[0287] In Example 37, the concentration of lactic acid in the test solution was 40 mM.

[0288] In Example 38, the concentration of lactic acid in the test solution was 50 mM.

[0289] Example 39

[0290] Urea detection solution is dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a urea detection section is formed. The urea detection solution includes p-dimethylaminobenzaldehyde alcohol solution, potassium hydrogen sulfate aqueous solution, and citrate-disodium hydrogen phosphate buffer solution; the pore size of the glass fiber filter paper is approximately 1 micrometer, and the thickness is approximately 2 mm.

[0291] Urea test solution contains 25 mg / mL -1 4 μL p-dimethylaminobenzaldehyde alcohol solution, 60 mg mL -1 3 μL potassium hydrogen sulfate aqueous solution, 20 mmol / L -13 μL citrate-disodium hydrogen phosphate buffer.

[0292] Add the solution to be tested to the urea detection unit. The urea concentration in the urea detection unit is 0.

[0293] The difference between Examples 40 to 44 and Example 39 is that the concentration of urea in the test solution is different.

[0294] In Example 40, the concentration of urea in the test solution was 5 mM.

[0295] In Example 41, the concentration of urea in the test solution was 10 mM.

[0296] In Example 42, the concentration of urea in the test solution was 20 mM.

[0297] In Example 43, the concentration of urea in the test solution was 30 mM.

[0298] In Example 44, the concentration of urea in the test solution was 40 mM.

[0299] Example 45

[0300] A pH detection solution was dropped onto glass fiber filter paper with a diameter of 3 mm, and after drying, a pH detection section was formed. The pH detection solution included an aqueous solution of alizarin red and an aqueous solution of hexadecyltrimethylammonium bromide. The glass fiber filter paper had a pore size of approximately 1 micrometer and a thickness of approximately 2 mm.

[0301] pH test solution contains 5 mg mL -1 2 μL alizarin red aqueous solution, 5 mg mL -1 8 μL of hexadecyltrimethylammonium bromide aqueous solution.

[0302] The solution to be tested is added to the pH detection unit. The pH value of the solution to be tested is 4.

[0303] The difference between Examples 46 to 50 and Example 45 is that the pH value of the test solution is different.

[0304] In Example 46, the pH value of the test solution was 5.

[0305] In Example 47, the pH value of the test solution was 6.

[0306] In Example 48, the pH value of the test solution was 7.

[0307] In Example 49, the pH value of the test solution was 8.

[0308] In Example 50, the pH value of the test solution was 9.

[0309] Comparative Example

[0310] Comparative Example 1 differs from Example 1 in the preparation process and materials of the test strip. In Comparative Example 1, a polyethylene glycol (PEO) nanofiber matrix was deposited onto the sensing area using electrospinning to form a nanofiber film.

[0311] Comparative Example 2 differs from Example 1 in the fabrication process and materials of the test strip. In Comparative Example 2, a soft microfluidic device is formed in a low-modulus silicone elastomer using soft photolithography.

[0312] Test example:

[0313] A camera is used to capture the colors of the colorimetric detection unit.

[0314] Results analysis:

[0315] Based on the examples and Comparative Examples 1 and 2, it can be concluded that: Example 1 can prepare a detection patch by simply combining common paper-based materials such as glass fiber filter paper, silicone paper, and release paper using a simple cutting method. The detection patch production process is simple and does not rely on large-scale equipment. In Comparative Example 1, PEO nanofiber matrix is ​​deposited onto the sensing area using electrospinning to form a nanofiber film. The production process relies on large-scale equipment. In Comparative Example 2, a soft microfluidic device is formed in a low-modulus silicone elastomer using soft photolithography. The production process is complex.

[0316] Reference Figure 3 , Figure 3 The numbers correspond to the order of Examples 2-7. According to Examples 2-7, the mass ratio of the materials in the glucose detection unit affects the color development. Among them, the color development of the glucose detection unit is more obvious in Examples 2 and 5.

[0317] Reference Figure 4 , Figure 4 The numbers correspond to the order of Examples 8-13. According to Examples 8-13, the mass ratio of the materials in the lactic acid detection section affects the color development. Among them, the color development of the lactic acid detection section is more obvious in Examples 8 and 11.

[0318] Reference Figure 5 , Figure 5 The numbers correspond to the order of Examples 14-19. According to Examples 14-19, the mass ratio of the materials in the urea detection section affects the color development. Among them, the urea detection section shows more obvious color development in Examples 14 and 17.

[0319] Reference Figure 6 , Figure 6The numbers correspond to the order of Examples 20-25. In Examples 20-25, the mass ratio of the materials in the pH detection section affects the color development. Among them, the urea detection section shows more obvious color development in Examples 20 and 23.

[0320] Refer to Table 1 and Figure 7 As shown, Figure 7 The results, from left to right, represent the detection results of Examples 26-32. In Examples 26-32, the glucose content in the test solution gradually increases. Figure 7 The numbers in the diagram correspond to the glucose concentration in the test solution. In Examples 26-32, the glucose detection unit exhibits noticeable color differences when the glucose content in the test solution varies, which are visually apparent. The glucose detection unit has a detection range of 0-0.3 mM, covering the glucose concentration range of human sweat. It offers high detection accuracy.

[0321] Reference Figure 8 , Figure 8 The results, from left to right, represent the detection results of Examples 33-38. In Examples 33-38, the lactic acid content in the test solution gradually increases. Figure 8 The numbers in the table correspond to the concentration of lactic acid in the test solution. In Examples 33-38, when the lactic acid content in the test solution varied, the color difference of the lactic acid detection section was significant and visually discernible. The detection accuracy of the lactic acid detection section was high. The detection range covered the range of lactic acid concentrations in human sweat.

[0322] Reference Figure 9 , Figure 9 The results, from left to right, represent the detection results of Examples 39-44. In Examples 39-44, the urea content in the test solution gradually increases. Figure 9 The numbers in the diagram correspond to the concentration of urea in the test solution. In Examples 39 and 44, the color difference of the urea detection unit is visually noticeable when the urea content in the test solution is different. The urea detection unit has high detection accuracy. The detection range of the urea detection unit covers the range of urea concentrations in human sweat.

[0323] Reference Figure 10 , Figure 10 The results from left to right in the middle represent the detection results of Examples 45-50. In Examples 45-50, the pH of the test solution gradually increases. Figure 10 The numbers in the diagram correspond to the pH values ​​in the test solution. In Examples 45-50, the pH detection unit exhibits significant color differences when the pH of the test solution varies, and these color differences are visually perceptible. The pH detection unit demonstrates high accuracy. Its detection range covers the pH range of human sweat.

[0324] Finally, it should be noted that other embodiments of the invention will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This invention is intended to cover any variations, uses, or adaptations of the invention that follow the general principles of the invention and include common knowledge or customary techniques in the art not disclosed herein, and is not limited to the precise structures described above and shown in the accompanying drawings, and various modifications and changes can be made without departing from its scope. The scope of the invention is limited only by the appended claims.

Claims

1. A detection patch, characterized in that, include: Grassroots (100); A colorimetric detection layer (200) is disposed on one side of the base layer (100) in the thickness direction; the colorimetric detection layer (200) includes a carrier and a colorimetric detection unit, the colorimetric detection unit being disposed on the side of the carrier opposite to the base layer (100); Both the base layer (100) and the colorimetric detection layer (200) are paper base layers.

2. The detection patch according to claim 1, characterized in that, It also includes a permeation layer (300) disposed between the colorimetric detection layer (200) and the base layer (100).

3. The detection patch according to claim 2, characterized in that, It also includes a transparent overlay layer (500) disposed on the side of the colorimetric detection layer (200) away from the base layer (100).

4. The detection patch according to claim 3, characterized in that, It also includes an isolation layer (400) disposed between the permeable layer (300) and the base layer (100); The isolation layer (400) is provided with mounting holes, and the permeable layer (300) contacts the base layer (100) through the mounting holes.

5. The detection patch according to claim 4, characterized in that, The isolation layer (400) and / or the permeable layer (300) are paper base layers.

6. The detection patch according to any one of claims 1-5, characterized in that, The number of colorimetric detection units is multiple; And / or, the plurality of colorimetric detection units are arranged at intervals along the extension plane direction of the carrier; And / or, the multiple colorimetric detection units are made of different materials and are used to detect different substances.

7. The detection patch according to claim 2, characterized in that, The permeable layer (300) has a first pore, the pore diameter D1 of which satisfies: 8μm≤D1≤15μm.

8. The detection patch according to claim 2, characterized in that, The thickness of the permeation layer (300) is P1, and P1 satisfies: 0.15≤P1≤0.25mm.

9. The detection patch according to claim 2, characterized in that, The colorimetric detection unit has a second pore, and the pore diameter D2 of the second pore satisfies: 0.5μm≤D2≤1.5μm.

10. The detection patch according to claim 2, characterized in that, The thickness of the colorimetric detection unit is P2, and P2 satisfies: 1.5mm≤P2≤2.5mm.

11. The detection patch according to claim 4, characterized in that, The thickness of the isolation layer (400) is P3, and P3 satisfies: 0.05mm≤P3≤0.15mm.

12. The detection patch according to claim 5, characterized in that, At least one of the following constraints must be met: The transparent overlay (500) is made of polyethylene terephthalate, polyethylene, or polypropylene. The carrier includes silicone paper; The colorimetric detection unit includes glass fiber filter paper; The permeation layer (300) includes cotton fiber filter paper; The isolation layer (400) includes silicone paper; The base layer (100) includes release paper.

13. The detection patch according to any one of claims 1-5, characterized in that, The colorimetric detection unit includes a glucose detection unit, a lactic acid detection unit, a urea detection unit, and a pH detection unit; The glucose detection unit, the lactic acid detection unit, the urea detection unit, and the pH detection unit are arranged in an array on the carrier.

14. The detection patch according to claim 13, characterized in that, The materials of the glucose detection unit include a glucose catalyst, a peroxide catalyst, a colorimetric reagent, and a pH reagent.

15. The detection patch according to claim 14, characterized in that, The glucose catalyst includes glucose oxidase; And / or, the peroxide catalyst includes horseradish peroxidase; And / or, the colorimetric agent comprises 3,3'-dimethoxybenzidine; And / or, the pH reagent includes citrate-disodium hydrogen phosphate buffer.

16. The detection patch according to claim 13, characterized in that, The materials of the lactic acid detection unit include a lactic acid catalyst, a peroxide catalyst, a colorimetric reagent, and a pH reagent.

17. The detection patch according to claim 16, characterized in that, The lactic acid catalyst includes lactate oxidase; And / or, the peroxide catalyst includes peroxidase; And / or, the colorimetric agent comprises 4-aminoantipyrine; And / or, the pH reagent includes citrate-disodium hydrogen phosphate buffer.

18. The detection patch according to claim 13, characterized in that, The materials used in the urea detection unit include p-dimethylaminobenzaldehyde, sulfate, and pH reagent.

19. The detection patch according to claim 18, characterized in that, The sulfate includes potassium hydrogen sulfate; And / or, the pH reagent includes citrate-disodium hydrogen phosphate buffer.

20. The detection patch according to claim 13, characterized in that, The materials of the pH detection unit include pH indicator and stabilizer.

21. The detection patch according to claim 20, characterized in that, The pH indicator includes alizarin red; And / or, the stabilizer includes hexadecyltrimethylammonium bromide.

22. The detection patch according to claim 14, characterized in that, The materials of the glucose detection unit include glucose oxidase, horseradish peroxidase, and 3,3'-dimethoxybenzidine; the mass ratio of glucose oxidase, horseradish peroxidase, and 3,3'-dimethoxybenzidine is (4-30):(1-20):(1-8).

23. The detection patch according to claim 16, characterized in that, The materials of the lactate detection unit include lactate oxidase, peroxidase, and 4-aminoantipyrine; the mass ratio of lactate oxidase, peroxidase, and 4-aminoantipyrine is (1-20):(2-40):(7-30).

24. The detection patch according to claim 18, characterized in that, The materials of the urea detection unit include p-dimethylaminobenzaldehyde and potassium hydrogen sulfate; the mass ratio of p-dimethylaminobenzaldehyde to potassium hydrogen sulfate is (3-20):(1-40).

25. The detection patch according to claim 20, characterized in that, The materials of the pH detection unit include alizarin red and hexadecyltrimethylammonium bromide; the mass ratio of alizarin red to hexadecyltrimethylammonium bromide is (1-20):(1-35).

26. The detection patch according to any one of claims 4-5, characterized in that, It also includes a first connector; the first connector is disposed on the side of the permeable layer (300) near the isolation layer (400), and the permeable layer (300) and the isolation layer (400) are connected by the first connector.

27. The detection patch according to any one of claims 2-5, characterized in that, It also includes a second connector; the second connector is disposed on the side of the colorimetric detection layer (200) near the permeation layer (300), and the colorimetric detection layer (200) and the permeation layer (300) are connected by the second connector.

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