An apparatus for culturing gut flora
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-20
- Publication Date
- 2026-06-23
Smart Images
Figure CN122256111A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of gut microbiota culture technology, and more specifically to an intestinal microbiota culture device. Background Technology
[0002] The gut microbiota is a complex community of microorganisms that colonizes the human gut. It is diverse and abundant, participating in several key physiological processes, including nutrient metabolism, intestinal mucosal barrier construction, and immune regulation. Imbalances in the gut microbiota are closely related to the development of various diseases such as enteritis, metabolic syndrome, and immune disorders. Currently, in vitro culture of gut microbiota typically involves sample pretreatment, primary anaerobic resuscitation, targeted enrichment culture, purification and identification, or long-term dynamic culture. Laboratory equipment commonly used includes anaerobic jars and anaerobic workstations to establish a basic anaerobic environment and activate the gut microbiota in a short period.
[0003] Among the widely used intestinal flora culture devices on the market, such as stirred anaerobic bioreactors, this device mainly consists of a sealed tank, an internal stirring paddle, an anaerobic gas supply system, and a constant temperature control module. Its working principle is to continuously introduce a mixture of nitrogen and carbon dioxide anaerobic gas into the sealed tank to completely expel the oxygen inside the tank, thereby creating an anaerobic environment that meets the growth requirements of intestinal flora. At the same time, the uniform rotation of the stirring paddle drives the culture system (including intestinal flora and nutrient substrate) in the tank to mechanically mix, so that the nutrients are evenly dispersed. With the constant temperature control module maintaining a human body simulated temperature of about 37°C, the in vitro batch culture of intestinal flora can be realized. However, the stirring method of this type of stirred anaerobic bioreactor is only a single uniform rotation. The dynamic environment it provides differs greatly from the real physiological environment of the intestine. It is difficult to simulate the physiological movement characteristics of the human intestine, which can easily lead to deviations in the structure, composition, and metabolic activity of the in vitro cultured flora from the real in vivo intestinal flora, affecting the reference value of the experimental results and subsequent translational applications.
[0004] Therefore, the present invention proposes an intestinal flora culture device to solve the above problems. Summary of the Invention
[0005] To address the aforementioned issues, this invention provides an intestinal flora culture device for in vitro anaerobic culture of intestinal flora. This device simulates the physiological motility characteristics of the human intestinal tract, optimizes the dynamic culture environment, improves the compatibility between in vitro cultured flora and real in vivo flora, and ensures the reference value of experimental results and their subsequent transformation and application effects.
[0006] To achieve the above objectives, the technical solution of the present invention is as follows: an intestinal flora culture device, comprising a box and a controller, the controller being installed on the surface of the box, the inner part of the box being a culture zone and a gas supply zone, the gas supply zone being provided with an anaerobic component for continuously supplying anaerobic gas to the culture zone, the anaerobic component being electrically connected to the controller, the culture zone being provided with a temperature control component for adjusting the temperature of the culture zone, the temperature control component being electrically connected to the controller, a fixed seat being fixedly connected to the top wall of the culture zone, a connecting cylinder being hinged to the fixed seat, and connecting rods being symmetrically fixedly connected to the surface of the connecting cylinder, and the plane of the two connecting rods being perpendicular to the axis of the connecting cylinder;
[0007] The culture area contains a culture body, and the end of the connecting rod away from the connecting cylinder is hinged to the culture body. The inside of the culture body is divided into a swing chamber and a culture chamber from top to bottom. The swing chamber is equipped with a swing component that is electrically connected to the controller. The swing component is used to change the center of gravity of the culture body so that the culture body can swing in a compound manner. The surface of the culture body is provided with a feed port that communicates with the culture chamber.
[0008] The technical principle of the above scheme is as follows: This device uses a controller as its core to achieve fully automated control of the entire process. First, the anaerobic component in the gas supply zone continuously supplies anaerobic gas to the culture zone under the controller's command. Simultaneously, the temperature control component adjusts the temperature of the culture zone, jointly constructing a constant-temperature, anaerobic basic culture environment that meets the growth requirements of intestinal flora. The culture zone forms a multi-node hinged movable support structure through a fixed seat, connecting cylinder, and connecting rod, providing a mechanical basis for the swinging of the culture body. The hinged spatial structure allows the culture body to have multi-dimensional degrees of freedom of movement. The controller drives the swing component in the swing chamber to work, changing the center of gravity distribution of the culture body, causing the culture body to generate multi-dimensional composite swinging in three-dimensional space based on the hinged structure, simulating the physiological movement characteristics of the human intestine.
[0009] The above-mentioned scheme has the following beneficial effects: This scheme accurately simulates the physiological movement characteristics of the intestine through compound oscillation, reducing the deviation between the in vitro cultured microbiota and the real in vivo microbiota in terms of structural composition and metabolic activity, thereby improving the reference value of experimental results and their potential for subsequent transformation and application. Simultaneously, the multi-node hinged movable support structure, combined with the compound oscillation achieved through center-of-gravity adjustment, replaces traditional mechanical stirring, resulting in gentler and more uniform mixing, reducing mechanical damage to the microbiota from the stirring paddle, and effectively ensuring the activity of the microbiota. Furthermore, the controller-based automated coordinated regulation of anaerobic and temperature control can construct a stable constant-temperature anaerobic culture environment. The feed inlet can also replenish nutrient substrates as needed, adapting to the long-term dynamic culture requirements of intestinal microbiota. The overall structure of the device is rationally designed, easy to operate, and possesses excellent practicality and adaptability.
[0010] Furthermore, the swing assembly includes a dual-output shaft motor fixedly connected inside the swing cavity, with the output shafts of the dual-output shaft motor distributed along the axis of the connecting cylinder; both ends of the output shafts of the dual-output shaft motor are coaxially and fixedly connected to swing rods that cooperate with the rotation of the culture body, and the ends of the swing rods away from the dual-output shaft motors extend outside the swing cavity and are fixedly connected to swing blocks, all of which are semi-circular structures, and the initial position phase difference between the two swing blocks is 180°; the dual-output shaft motor is electrically connected to the controller.
[0011] Beneficial effects: The semi-circular oscillating block, with a 180° phase difference, continuously and regularly alters the center of gravity distribution of the culture medium as it rotates with the oscillating rod. This makes the composite oscillation of the culture medium more closely resemble the physiological movements of human intestinal peristalsis and torsion, resulting in more uniform mixing of the bacterial flora and nutrient substrate within the culture chamber. Simultaneously, this component achieves oscillation through center of gravity adjustment, eliminating the contact between the rigid stirring structure and the culture system, reducing physical damage to the bacterial flora caused by mechanical stirring, and effectively protecting bacterial activity.
[0012] Furthermore, the surface of the culture medium is symmetrically and fixedly connected with protective covers for shielding the swing blocks.
[0013] Beneficial effects: The protective cover can effectively protect the rotating oscillating block from collision damage during operation, prevent external impurities from contacting the oscillating block, and reduce airflow disturbance caused by the rotation of the oscillating block, thus ensuring the stability of the anaerobic environment in the culture area.
[0014] Furthermore, several disturbance rods are fixedly connected to the bottom of the culture chamber, and the top of each disturbance rod is fixedly connected to the top of the culture chamber; a disturbance support is provided inside the culture chamber and is fixedly connected to the disturbance rods.
[0015] Beneficial effects: During the composite oscillation of the culture body, the disturbance rod and disturbance support can create multi-directional disturbances to the culture system within the culture chamber, allowing the microbial community and nutrient substrate to mix more thoroughly and evenly, while effectively preventing the microbial community from settling and accumulating at the bottom of the culture chamber. Furthermore, the overall structure is fixed, with no additional mechanical movement, reducing physical damage to the microbial community and ensuring its activity without affecting the normal oscillation of the culture body.
[0016] Furthermore, the temperature control component includes several electrically controlled heating columns fixedly connected to the inner wall of the culture zone, and each of the electrically controlled heating columns is electrically connected to the controller; a temperature sensor electrically connected to the controller is fixedly connected to the bottom wall of the culture zone.
[0017] Beneficial effects: The electrically controlled heating column is attached to the inner wall of the culture area, resulting in more uniform heating. Combined with a temperature sensor to monitor the temperature of the culture area in real time, it forms a closed-loop temperature control with the controller, accurately maintaining the constant temperature environment required for the growth of intestinal flora. The temperature control has a fast response and high accuracy, which is suitable for the growth needs of intestinal flora.
[0018] Furthermore, the anaerobic assembly includes a gas storage tank and a gas recovery tank, both of which are fixedly connected to the bottom wall of the gas supply area. An air pump is fixedly connected to the top of the gas storage tank, with its input end connected to the inside of the gas storage tank and its output end connected to an air inlet pipe. A recovery pump is fixedly connected to the top of the gas recovery tank, with its input end connected to an exhaust pipe and its output end connected to the inside of the gas recovery tank. Both the exhaust pipe and the air inlet pipe are connected to the culture area. Both the air pump and the recovery pump are electrically connected to the controller.
[0019] Beneficial effects: The air pump and the recovery pump work together to realize the circulation supply and recovery of anaerobic gas in the culture zone, which can sustainably maintain the stability of the anaerobic environment in the culture zone, reduce gas waste, and, when linked with the controller, can precisely control the gas on / off and flow rate to meet the anaerobic needs of the entire microbial culture process.
[0020] Furthermore, a strain component is fixedly connected to the bottom of the culture body to adjust the swing amplitude of the culture body according to the intestinal flora culture time, and the swing amplitude of the culture body is proportional to the gas pressure in the culture chamber.
[0021] Beneficial effects: The swing amplitude of the culture medium is dynamically adjusted according to the air pressure in the culture chamber (i.e. the culture time of the intestinal flora), which precisely adapts to the movement environment requirements of the flora at different growth stages. During the initial recovery period, the swing amplitude is small to avoid mechanical impact damage to the flora, while the swing amplitude is increased during the proliferation and stabilization period to improve the efficiency of nutrient mixing and metabolic exchange.
[0022] Furthermore, the strain assembly includes a strain seat fixedly connected to the bottom of the culture body. The strain seat has a sliding cavity inside, and a strain rod is slidably fitted onto the inner wall of the sliding cavity. The bottom end of the strain rod passes through the bottom wall of the sliding cavity and is located below the bottom of the strain seat. The sliding cavity is divided into a balance zone and a buffer zone from top to bottom by the strain rod. Several springs are installed in the buffer zone, with both ends of the springs fixedly connected to the strain rod and the bottom wall of the sliding cavity, respectively. A guide channel is provided inside the culture body, with both ends connecting to the culture cavity and the buffer zone, respectively. The end of the guide channel closest to the culture cavity is located on the top wall of the culture cavity. A curved guide groove is formed on the bottom wall of the culture zone. The bottom end of the strain rod is always in contact with the inner surface of the guide groove.
[0023] Beneficial effects: Metabolic gases (such as carbon dioxide) in the culture chamber enter the buffer zone through the guide channel as the culture time progresses. The spring is gradually stretched to change the extension length of the strain rod, which in turn changes the contact pressure to change the magnitude of the friction force that needs to be overcome during the swing. Combined with the curved guide groove on the bottom wall of the culture zone, the swing amplitude can be adjusted proportionally to the culture time, precisely adapting to the needs of small swings during the bacterial colony recovery period and large swings during the stable proliferation period.
[0024] Furthermore, the bottom of the strain gauge has a dome structure; and the inner wall of the guide groove is provided with a rubber layer.
[0025] Beneficial effects: The dome structure at the bottom of the strain gauge prevents jamming from affecting the amplitude adjustment; at the same time, the rubber layer on the inner wall of the guide groove can buffer contact impact, reduce operating noise, reduce component wear, extend the service life of the structure, and ensure the smoothness of amplitude adjustment.
[0026] Furthermore, the section of the guide channel near the culture chamber has a "∩" shaped structure.
[0027] Beneficial effects: The "∩" shaped structure of the guide channel can form an effective barrier, allowing only gas from the culture chamber to flow in, preventing liquid from entering the guide channel and causing blockage, ensuring smooth opening and closing of the guide channel, and ensuring the stable realization of automatic amplitude adjustment.
[0028] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description
[0029] Figure 1 This is an overall isometric view of an embodiment of the gut microbiota culture device of the present invention;
[0030] Figure 2 This is an overall top view of an embodiment of the intestinal flora culture device of the present invention;
[0031] Figure 3 for Figure 2 Sectional view of mid-section line AA;
[0032] Figure 4 for Figure 2 Sectional view of the mid-section line BB;
[0033] Figure 5 This is an isometric view of the oscillating component of an embodiment of the intestinal flora culture device of the present invention;
[0034] Figure 6 This is an isometric view of the oscillating component of an embodiment of the intestinal flora culture device of the present invention;
[0035] Figure 7 This is a schematic diagram of the swing block in an embodiment of the intestinal flora culture device of the present invention;
[0036] Figure 8 for Figure 7 Enlarged view of section C;
[0037] Figure 9 This is a schematic diagram of the guide channel for an embodiment of the intestinal flora culture device of the present invention.
[0038] The reference numerals in the accompanying drawings of the instruction manual include: 1. Box body; 101. Culture zone; 102. Gas supply zone; 2. Controller; 3. Gas storage tank; 301. Air pump; 302. Air inlet pipe; 4. Gas recovery tank; 401. Recovery pump; 402. Exhaust pipe; 5. Culture body; 501. Feed inlet; 502. Swing block; 503. Swing rod; 504. Swing chamber; 505. Dual-shaft motor; 506. Culture chamber; 507. Disturbance rod; 6. Connecting rod; 7. Connecting cylinder; 8. Fixing base; 9. Electrically controlled heating column; 10. Guide groove; 11. Temperature sensor; 12. Strain gauge; 1201. Buffer zone; 1202. Spring; 1203. Strain rod; 1204. Guide channel; 13. Protective cover. Detailed Implementation
[0039] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0040] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.
[0041] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.
[0042] The following detailed description illustrates the specific implementation method:
[0043] Example 1:
[0044] like Figure 1 As shown, an intestinal flora culture device includes a housing 1 and a controller 2 integrally formed on the surface of the housing 1. Figure 2 and Figure 3 As shown, the interior of the chamber 1 is divided into a culture zone 101 and a gas supply zone 102.
[0045] Combination Figure 3 and Figure 4 As shown, a gas storage tank 3 and a gas recovery tank 4 are bolted together in the gas supply area 102. An inflation pump 301 is screwed to the top of the gas storage tank 3, with its input end connected to the interior of the gas storage tank 3 and its output end connected to an inlet pipe 302. A recovery pump 401 is screwed to the top of the gas recovery tank 4, with its input end connected to an exhaust pipe 402 and its output end connected to the interior of the gas recovery tank 4. Both the exhaust pipe 402 and the inlet pipe 302 are connected to the culture area 101. Both the inflation pump 301 and the recovery pump 401 are electrically connected to the controller 2. Simultaneously, several electrically controlled heating columns 9, electrically connected to the controller 2, are screwed to the inner wall of the culture area 101, and a temperature sensor 11, electrically connected to the controller 2, is screwed to the bottom wall of the culture area 101.
[0046] like Figure 5 As shown, a fixed base 8 is integrally formed on the top wall of the culture zone 101. A connecting cylinder 7 is hinged to the fixed base 8. Connecting rods 6 are integrally formed symmetrically on the surface of the connecting cylinder 7, and the planes of the two connecting rods 6 are perpendicular to the axis of the connecting cylinder 7. A culture body 5 is disposed within the culture zone 101, and the bottom ends of the connecting rods 6 are all hinged to the culture body 5 (e.g., ...). Figure 5 (As shown). Combined Figure 7 As shown, the culture body 5 is divided into a swing chamber 504 and a culture chamber 506 from top to bottom. A dual-axis motor 505, electrically connected to the controller 2, is screwed into the swing chamber 504. The output shafts of the dual-axis motor 505 are distributed along the axis of the connecting cylinder 7. Both ends of the dual-axis motor 505 are coaxially screwed to swing rods 503 that rotate with the culture body 5. The ends of the swing rods 503 away from the dual-axis motor 505 extend outside the swing chamber 504 and are screwed to swing blocks 502. The swing blocks 502 are all semi-circular structures, and the initial phase difference between the two swing blocks 502 is 180° (e.g., ...). Figure 6As shown in the figure), the surface of the culture body 5 is provided with an inlet 501 that communicates with the culture chamber 506. An electrically controlled three-way valve (not shown in the figure) electrically connected to the controller 2 is installed at the inlet 501. The three ports of this electrically controlled three-way valve are respectively connected to the culture chamber 506, the external liquid supply end, and the culture area 101. When culture medium is injected into the culture chamber 506, the electrically controlled three-way valve connects the culture chamber 506 to the external liquid supply end. After a specified amount is injected, it switches to connect the culture chamber 506 to the culture area 101, realizing rapid oxygen replacement within the culture chamber 506. Simultaneously, protective covers 13 for covering the swing block 502 are symmetrically screwed onto the surface of the culture body 5. Additionally, several disturbance rods 507 are welded to the bottom of the culture chamber 506, with the tops of the disturbance rods 507 welded to the top of the culture chamber 506. A disturbance support integrally formed with the disturbance rods 507 is provided inside the culture chamber 506.
[0047] In addition, combined Figure 7 , Figure 8 and Figure 9 As shown, the bottom of the culture body 5 is integrally formed with a strain seat 12. The strain seat 12 has a sliding cavity inside, and a strain rod 1203 is slidably fitted on the inner wall of the sliding cavity. The bottom end of the strain rod 1203 passes through the bottom wall of the sliding cavity and is located below the bottom end of the strain seat 12. The sliding cavity is divided into a balance zone and a buffer zone 1201 from top to bottom by the strain rod 1203. Several springs 1202 are provided in the buffer zone 1201. The two ends of the springs 1202 are fixedly connected to the strain rod 1203 and the bottom wall of the sliding cavity, respectively. The culture body 5 is provided with a guide channel 1204. The two ends of the guide channel 1204 are connected to the culture cavity 506 and the buffer zone 1201, respectively. The end of the guide channel 1204 near the culture cavity 506 is located on the top wall of the culture cavity 506. The section of the guide channel 1204 near the culture cavity 506 has a "∩" shaped structure. The bottom wall of the cultivation zone 101 has a curved guide groove 10; the bottom end of the strain rod 1203 is always in contact with the inner surface of the guide groove 10. At the same time, the bottom end of the strain rod 1203 has a dome structure; the inner wall of the guide groove 10 is provided with a rubber layer.
[0048] The specific implementation process is as follows: culture medium is injected through the feed inlet 501 of the culture body 5. After the feed inlet 501 is closed, the controller 2 on the surface of the chamber 1 completes the parameter preset (for example, setting the temperature control target to 37℃, the anaerobic gas circulation flow rate to 0.5L / min, and the initial speed of the dual-shaft motor 505 to 10r / min, so as to build the basic operating conditions for the culture).
[0049] Subsequently, the aeration pump 301 pumps the N2-CO2 mixed anaerobic gas from the gas storage tank 3 into the culture zone 101 through the inlet pipe 302. Simultaneously, the recovery pump 401 returns the gas from the culture zone 101 to the gas recovery tank 4 through the exhaust pipe 402, forming a closed-loop anaerobic gas circulation. This rapidly discharges oxygen from the culture zone 101 and the culture chamber 506, continuously maintaining the anaerobic environment. After the oxygen replacement in the culture chamber 506 is completed, the connection between the culture chamber 506 and the culture zone 101 is severed. At the same time, the electrically controlled heating column 9 starts heating, and the temperature sensor 11 in the culture zone 101 collects temperature change data in real time and feeds it back to the controller 2, forming a closed-loop temperature control. If the temperature is below 36.5℃, the heating column automatically heats up; if it is above 37.5℃, heating stops, precisely maintaining the culture chamber 506 at 37℃, a simulated human intestinal temperature, to meet the basic environmental requirements for bacterial growth.
[0050] After the culture zone 101 reaches the constant temperature anaerobic conditions, the controller 2 drives the dual-output shaft motor 505 in the swing chamber 504 to operate. The output shaft of the dual-output shaft motor 505 synchronously drives the swing rods 503 at both ends to rotate at a uniform speed along the axis of the connecting cylinder 7. Since the initial phase difference of the two semi-circular swing blocks 502 is 180°, the center of gravity distribution of the culture body 5 will be continuously and regularly changed during the rotation. Relying on the multi-node hinged movable support structure of the fixed seat 8, the connecting cylinder 7 and the connecting rod 6, the culture body 5 produces multi-dimensional composite swing, simulating the physiological movement characteristics of human intestinal peristalsis and torsion. The protective cover 13 on the surface of the culture body 5 effectively protects the rotating swing block 502, avoids damage from component collisions, and reduces airflow disturbance caused by the rotation of the swing block 502, further ensuring the stability of the anaerobic environment in the culture zone 101. When the culture body 5 is oscillating, the culture medium in the culture chamber 506 is shaken, which causes the culture medium to move relative to the disturbance rod 507 and disturbance support in the culture chamber 506 (i.e., to achieve a stirring effect). This creates multi-directional non-contact disturbance to the culture system. Combined with the oscillation, the bacteria are mixed more thoroughly with the culture medium, effectively reducing the sedimentation and accumulation of bacteria at the bottom of the chamber. Furthermore, since there is no rigid stirring structure in contact with the culture system, mechanical damage is reduced, and the initial survival rate of the bacteria is improved.
[0051] In the early stage of cultivation (0-24h is the initial recovery period of the intestinal flora), the intestinal flora has weak activity and slow proliferation rate. The amount of carbon dioxide produced by anaerobic respiration is very small. The air pressure in the culture chamber 506 and the buffer zone 1201 is kept in balance. The spring 1202 is naturally extended, and the strain rod 1203 is in the extended state. The bottom of the dome of the strain rod 1203 is in full contact with the rubber layer of the guide groove 10. The friction is relatively large, so that the culture body 5 can only produce a small swing (swing angle of about 5°). This can effectively avoid impacting the weakly active recovery period flora and ensure the initial survival rate of the flora.
[0052] As the cultivation time progresses, the bacterial community enters a stable proliferation phase between 24 and 72 hours, with a significant increase in bacterial count and a marked enhancement in anaerobic respiration, leading to a surge in carbon dioxide production and increased internal pressure in the culture chamber 506. This high-pressure gas is transmitted to the buffer zone 1201 via the "∩"-shaped guide channel 1204 on the top wall of the culture chamber 506. This generates an upward thrust on the extended strain gauge 1203, causing it to retract and stretch the spring 1202. This reduces the contact pressure between the strain gauge 1203 and the surface of the guide groove 10, thus lowering the sliding friction. Combined with the curved structure of the guide groove 10, the reduced friction decreases the oscillation resistance of the culture body 5, automatically increasing the oscillation amplitude (swing angle to 15°). This improves the efficiency of nutrient transfer and metabolite diffusion, meeting the demands of high-density bacterial growth.
[0053] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.
Claims
1. An intestinal flora culture device, comprising a housing (1) and a controller (2), wherein the controller (2) is mounted on the surface of the housing (1), the housing (1) is divided into a culture zone (101) and a gas supply zone (102), the gas supply zone (102) is provided with an anaerobic component for continuously supplying anaerobic gas to the culture zone (101), the anaerobic component is electrically connected to the controller (2), and the culture zone (101) is provided with a temperature control component for adjusting the temperature of the culture zone (101), the temperature control component is electrically connected to the controller (2), characterized in that, A fixed seat (8) is fixedly connected to the top wall of the cultivation area (101). A connecting cylinder (7) is hinged to the fixed seat (8). Connecting rods (6) are symmetrically fixedly connected to the surface of the connecting cylinder (7), and the planes where the two connecting rods (6) are located are perpendicular to the axis of the connecting cylinder (7). The culture area (101) is provided with a culture body (5), and the end of the connecting rod (6) away from the connecting cylinder (7) is hinged to the culture body (5); the inside of the culture body (5) is divided into a swing cavity (504) and a culture cavity (506) from top to bottom; a swing component electrically connected to the controller (2) is installed in the swing cavity (504); the swing component is used to change the center of gravity position of the culture body (5) so that the culture body (5) can perform compound swing; the surface of the culture body (5) is provided with a feed port (501) that communicates with the culture cavity (506).
2. The intestinal flora culture device according to claim 1, characterized in that, The swing assembly includes a dual-axis motor (505) fixedly connected in the swing cavity (504). The output shaft of the dual-axis motor (505) is distributed along the axis of the connecting cylinder (7). Both ends of the output shaft of the dual-axis motor (505) are coaxially fixedly connected to swing rods (503) that rotate with the culture body (5). The ends of the swing rods (503) away from the dual-axis motor (505) extend to the outside of the swing cavity (504) and are fixedly connected to swing blocks (502). The swing blocks (502) are all semi-circular structures, and the initial position phase difference between the two swing blocks (502) is 180°. The dual-axis motor (505) is electrically connected to the controller (2).
3. The intestinal flora culture device according to claim 2, characterized in that, The surface of the culture body (5) is symmetrically fixed with a protective cover (13) for covering the swing block (502).
4. The intestinal flora culture device according to claim 3, characterized in that, The bottom of the culture chamber (506) is fixedly connected to a disturbance rod (507), and the top of the disturbance rod (507) is fixedly connected to the top of the culture chamber (506); a disturbance support is provided inside the culture chamber (506) and fixedly connected to the disturbance rod (507).
5. The intestinal flora culture device according to claim 4, characterized in that, The temperature control component includes several electrically controlled heating columns (9) fixedly connected to the inner wall of the culture zone (101), and the electrically controlled heating columns (9) are all electrically connected to the controller (2); a temperature sensor (11) electrically connected to the controller (2) is fixedly connected to the bottom wall of the culture zone (101).
6. The intestinal flora culture device according to claim 5, characterized in that, The anaerobic assembly includes a gas storage tank (3) and a gas recovery tank (4). Both the gas storage tank (3) and the gas recovery tank (4) are fixedly connected to the bottom wall of the gas supply area (102). An air pump (301) is fixedly connected to the top of the gas storage tank (3). The input end of the air pump (301) is connected to the inside of the gas storage tank (3), and the output end of the air pump (301) is connected to the air inlet pipe (302). A recovery pump (401) is fixedly connected to the top of the gas recovery tank (4). The input end of the recovery pump (401) is connected to the exhaust pipe (402), and the output end of the recovery pump (401) is connected to the inside of the gas recovery tank (4). The exhaust pipe (402) and the air inlet pipe (302) are both connected to the culture area (101). Both the air pump (301) and the recovery pump (401) are electrically connected to the controller (2).
7. The intestinal flora culture device according to claim 6, characterized in that, The bottom end of the culture body (5) is fixedly connected to a strain component for adjusting the swing amplitude of the culture body (5) according to the intestinal flora culture time, and the swing amplitude of the culture body (5) is proportional to the air pressure in the culture chamber (506).
8. The intestinal flora culture device according to claim 7, characterized in that, The strain assembly includes a strain seat (12) fixedly connected to the bottom of the culture body (5). The strain seat (12) has a sliding cavity inside, and a strain rod (1203) is slidably fitted on the inner wall of the sliding cavity. The bottom end of the strain rod (1203) passes through the bottom wall of the sliding cavity and is located below the bottom end of the strain seat (12). The sliding cavity is divided into a balance zone and a buffer zone (1201) from top to bottom by the strain rod (1203). Several springs (1202) are provided in the buffer zone (1201), and the two ends of the springs (1202) are fixedly connected to each other. The strain rod (1203) is attached to the bottom wall of the sliding cavity; the culture body (5) is provided with a guide channel (1204), the two ends of the guide channel (1204) are connected to the culture cavity (506) and the buffer zone (1201) respectively, and the end of the guide channel (1204) near the culture cavity (506) is located on the top wall of the culture cavity (506); the bottom wall of the culture area (101) is provided with a curved guide groove (10); the bottom end of the strain rod (1203) is always in contact with the inner surface of the guide groove (10).
9. The intestinal flora culture device according to claim 8, characterized in that, The bottom of the strain gauge (1203) is a dome structure; the inner wall of the guide groove (10) is provided with a rubber layer.
10. The intestinal flora culture device according to claim 9, characterized in that, The section of the guide channel (1204) near the culture chamber (506) has a "∩" shaped structure.