A method for promoting seed germination of smilax bracteata

Abstract

This invention discloses a method for promoting the germination of *Polygonatum yunnanense* seeds, relating to the field of plant cultivation technology. The method includes: S1. Treating *Polygonatum yunnanense* seeds with a composite enzyme solution containing pectinase and cellulase; S2. Subjecting the seeds to sequential variable-temperature cyclic culture and ultrasonic treatment; S3. Immersing the seeds in a composite solution containing GA₃, 6-BA, and an organic nitrogen source, while simultaneously removing floating seeds and transferring them to a GA₃ solution without 6-BA and the organic nitrogen source for further soaking; S4. Sowing the seeds in a germination substrate for cultivation. This invention, through the organic combination of physical, chemical, and biological methods and the refined sorting of seeds, systematically solves the problems of difficult and long germination cycles of *Polygonatum yunnanense* seeds, significantly improving germination rate and uniformity, and shortening germination time, showing promising application prospects.

Description

Technical Field

[0001] This invention belongs to the field of plant cultivation technology, specifically relating to a method for promoting the germination of Polygonatum yunnanense seeds. Background Technology

[0002] Yunnan Polygonatum ( Polygonatum kingianum Polygonatum yunnanense is an important plant used for both food and medicine, and its seeds possess a deep comprehensive dormancy characteristic. Belonging to the genus Polygonatum in the family Liliaceae, its rhizome is used medicinally. It has a mild, sweet taste and is easily taken, making it a superior tonic with functions of replenishing qi and yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. With a deeper understanding of the medicinal value and health benefits of Polygonatum yunnanense, wild resources are gradually being depleted, and artificial cultivation has become the main way to obtain it in the market. Artificial cultivation of Polygonatum yunnanense mainly includes sexual and asexual reproduction. Asexual reproduction primarily uses rhizomes, but the overall reproduction coefficient is low, the overall production cost is high, and the seedlings grown using rhizomes have poor uniformity, easily leading to losses in large-scale planting. Sexual reproduction of Polygonatum yunnanense is through seed cultivation, but Polygonatum yunnanense seeds have a long dormancy period and a low natural germination rate.

[0003] Currently, methods such as gibberellin soaking and low-temperature stratification are commonly used to break seed dormancy. However, these methods generally suffer from problems such as long treatment cycles, uneven germination, and poor effectiveness on low-vitality seeds. Existing technologies have not yet effectively integrated physical, chemical, and biological methods to systematically solve the multiple obstacles in the germination process of *Polygonatum yunnanense* seeds. Therefore, there is an urgent need in this field for an efficient, stable, and large-scale *Polygonatum yunnanense* seed germination method. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a method for promoting the germination of Polygonatum yunnanense seeds with a short germination cycle, high germination rate, and robust seedlings.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solution: A method for promoting the germination of Polygonatum yunnanense seeds mainly includes the following steps: S1. Select mature and plump seeds of Polygonatum yunnanense and treat them with a compound enzyme solution containing pectinase and cellulase. S2. The seeds treated in S1 were subjected to variable temperature cycling culture and ultrasonic treatment in sequence; S3. Soak the seeds treated in S2 in a compound solution containing gibberellin (GA3), cytokinin (6-BA) and organic nitrogen source. Seeds that float on the surface of the solution during soaking are removed and transferred to a gibberellin solution without 6-BA and organic nitrogen source for further soaking. S4. Sow the soaked seeds in the germination substrate for cultivation.

[0006] Furthermore, in step S1, before the compound enzyme solution treatment, the seeds are first disinfected with a 1-3% sodium hypochlorite solution for 10-20 minutes and then rinsed with sterile water.

[0007] Furthermore, the pectinase activity is 100,000 u / g, the cellulase activity is 5,000 u / g, and the dosage of both is 0.03-0.08% of the seed weight.

[0008] Furthermore, in step S2, after ultrasonic treatment, the seeds need to be soaked in warm water at 30-35℃.

[0009] Furthermore, the variable temperature cyclic culture consists of a daily cycle of 8 hours at 25°C and 16 hours at 5°C, lasting for 15-25 days.

[0010] Furthermore, in the composite solution, the concentration of gibberellin (GA3) is 50-150 mg / L, the concentration of cytokinin (6-BA) is 10-50 mg / L, and the concentration of organic nitrogen source is 5-15 g / L.

[0011] Furthermore, step S4 also includes a staged stress-resistant treatment, the conditions of which are: temperature 15-25℃, humidity 55-60%, lasting for 2 days.

[0012] Further, the germination substrate is any one of the following a) or b): a) 1 / 2MS solid culture medium with 0.5-1 g / L humic acid and 2-3 g / L perlite added; b) a composite substrate obtained by mixing humus, river sand and coconut coir in a volume ratio of 3:1:1 and sterilizing it.

[0013] The beneficial effects of this invention are: 1. This invention utilizes the synergistic effect of compound enzyme solution (biodegradation) and ultrasound (physical cavitation) to effectively soften and slightly damage the hard seed coat of Polygonatum yunnanense, thereby improving the subsequent absorption efficiency of water and hormones by Polygonatum yunnanense seeds. Combined with the precise breaking of physiological dormancy through 25℃ / 5℃ temperature cycling and the hormonal balance regulation of GA3 and 6-BA, the germination rate of Polygonatum yunnanense seeds ultimately reaches 90%-95%, which is nearly double that of the traditional GA3 soaking method (45%-48%).

[0014] 2. This invention accelerates embryo maturation by simulating the natural habitat through variable temperature cycling and activates the seed's endogenous enzyme system with ultrasound, thus advancing the seed germination start time to about 5 days after treatment. The entire germination cycle from seed treatment to germination completion is shortened to 30-40 days, which is more than 60% shorter than the traditional wet sand stratification method (90-120 days). This solves the core problem of "long treatment cycle" in the background technology and creates favorable conditions for early transplanting of *Polygonatum yunnanense* seedlings and seizing the growth cycle.

[0015] 3. The synergistic effect of organic nitrogen sources (peptone, yeast extract) and hormones provides a continuous supply of nutrients for seed germination. Combined with a germination substrate rich in humic acid, the cultivated seedlings have well-developed root systems and robust stems, resulting in a 20%-30% increase in seedling height compared to seedlings cultivated using traditional methods. Detailed Implementation

[0016] This invention discloses a method for promoting the germination of Polygonatum yunnanense seeds, which mainly includes the following steps: S1. Select mature and plump seeds of Polygonatum yunnanense and treat them with a compound enzyme solution containing pectinase and cellulase. S2. The seeds treated in S1 were subjected to variable temperature cycling culture and ultrasonic treatment in sequence; S3. Soak the seeds treated in S2 in a compound solution containing gibberellin (GA3), cytokinin (6-BA) and organic nitrogen source. Seeds that float on the surface of the solution during soaking are removed and transferred to a gibberellin solution without 6-BA and organic nitrogen source for further soaking. S4. Sow the soaked seeds in the germination substrate for cultivation.

[0017] In a preferred embodiment, in step S1, before the compound enzyme solution treatment, the seeds are first disinfected with a 1-3% sodium hypochlorite solution for 10-20 minutes and then rinsed with sterile water.

[0018] In a preferred embodiment, the pectinase activity is 100,000 u / g, the cellulase activity is 5,000 u / g, and the amount of both is 0.03-0.08% of the seed weight.

[0019] In a preferred embodiment, in step S2, after ultrasonic treatment, the seeds need to be soaked in warm water at 30-35℃.

[0020] In one preferred embodiment, the variable temperature cyclic culture is a daily cycle of 8 hours at 25°C and 16 hours at 5°C, lasting for 15-25 days.

[0021] In a preferred embodiment, the concentration of gibberellin (GA3) in the composite solution is 50-150 mg / L, the concentration of cytokinin (6-BA) is 10-50 mg / L, and the concentration of organic nitrogen source is 5-15 g / L.

[0022] In a preferred embodiment, step S4 further includes a staged stress-resistant treatment, wherein the stress-resistant treatment conditions are: temperature 15-25℃, humidity 55-60%, lasting for 2 days.

[0023] As a preferred embodiment, the germination substrate is any one of the following a) or b): a) 1 / 2MS solid culture medium with 0.5-1 g / L humic acid and 2-3 g / L perlite added; b) a composite substrate obtained by mixing humus, river sand and coconut coir in a volume ratio of 3:1:1 and sterilizing it.

[0024] The present invention will be further described below with reference to embodiments, but the scope of protection of the present invention is not limited thereto. Unless otherwise specified, the materials, reagents, etc. used in the embodiments can be obtained commercially.

[0025] Example 1 S1. Select mature, plump *Polygonatum yunnanense* seeds, disinfect them by soaking in a 2% sodium hypochlorite solution for 15 minutes, and then rinse them four times with sterile water; subsequently, place the seeds in a compound enzyme solution and soak them at 19℃ for 3 hours. The compound enzyme solution consists of pectinase with an activity of 100,000 u / g and cellulase with an activity of 5,000 u / g, both at 0.05% of the seed weight.

[0026] S2. The enzyme-treated seeds were subjected to variable-temperature cyclic culture, with the cycle conditions being 25℃ for 8 hours and 5℃ for 16 hours, for a total of 20 days. Immediately afterwards, the seeds were subjected to ultrasonic treatment at a power of 200W for 2 minutes. Immediately after treatment, the seeds were immersed in warm water at 33℃ for 8 minutes.

[0027] S3. Soak the seeds treated in S2 in a compound solution for 18 hours. The compound solution consisted of 100 mg / L GA3, 30 mg / L 6-BA, and 10 g / L peptone, with the pH adjusted to 6.0. After soaking began, the seeds that remained floating on the surface were removed and transferred to a 100 mg / L GA3 solution for the same soaking time.

[0028] S4. Sow the soaked seeds in 1 / 2 MS solid medium supplemented with 0.8 g / L humic acid and 2.5 g / L perlite. Culture under conditions of 20°C, 65% humidity, and a 12h light / 12h dark photoperiod (white LED light, 2500 Lux). On day 12 of culture, a phased stress-resistance treatment was performed, adjusting the environment to 20°C and 58% humidity for 2 days, after which the initial culture conditions were restored.

[0029] Comparative Example 1 S1. Select mature and plump seeds of Polygonatum yunnanense, soak them in a 2% sodium hypochlorite solution for 15 minutes for disinfection, and then rinse them 4 times with sterile water.

[0030] S2. The enzyme-treated seeds were subjected to variable-temperature cyclic culture, with the cyclic conditions being 25℃ for 8 hours and 5℃ for 16 hours, for a total of 20 days; then, they were subjected to ultrasonic treatment at a power of 200W for 2 minutes. Immediately after treatment, the seeds were immersed in warm water at 33℃ for 8 minutes.

[0031] S3. Soak the seeds treated in S2 in a compound solution for 18 hours. The compound solution consisted of 100 mg / L GA3, 30 mg / L 6-BA, and 10 g / L peptone, with the pH adjusted to 6.0. After soaking began, the seeds that remained floating on the surface were removed and transferred to a 100 mg / L GA3 solution for the same soaking time.

[0032] S4. Sow the soaked seeds in 1 / 2 MS solid medium supplemented with 0.8 g / L humic acid and 2.5 g / L perlite. Culture under conditions of 20°C, 65% humidity, and a 12h light / 12h dark photoperiod (white LED light, 2500 Lux). On day 12 of culture, a phased stress-resistance treatment was performed, adjusting the environment to 20°C and 58% humidity for 2 days, after which the initial culture conditions were restored.

[0033] Comparative Example 2 S1. Select mature, plump *Polygonatum yunnanense* seeds, disinfect them by soaking in a 2% sodium hypochlorite solution for 15 minutes, and then rinse them four times with sterile water; subsequently, place the seeds in a compound enzyme solution and soak them at 19℃ for 3 hours. The compound enzyme solution consists of pectinase with an activity of 100,000 u / g and cellulase with an activity of 5,000 u / g, both at 0.05% of the seed weight.

[0034] S2. The enzyme-treated seeds were subjected to variable-temperature cyclic culture, with the cyclic conditions being 25℃ for 8 hours and 5℃ for 16 hours, for a total of 20 days.

[0035] S3. Soak the seeds treated in S2 in a compound solution for 18 hours. The compound solution consisted of 100 mg / L GA3, 30 mg / L 6-BA, and 10 g / L peptone, with the pH adjusted to 6.0. After soaking began, the seeds that remained floating on the surface were removed and transferred to a 100 mg / L GA3 solution for the same soaking time.

[0036] S4. Sow the soaked seeds in 1 / 2 MS solid medium supplemented with 0.8 g / L humic acid and 2.5 g / L perlite. Culture under conditions of 20°C, 65% humidity, and a 12h light / 12h dark photoperiod (white LED light, 2500 Lux). On day 12 of culture, a phased stress-resistance treatment was performed, adjusting the environment to 20°C and 58% humidity for 2 days, after which the initial culture conditions were restored.

[0037] Comparative Example 3 S1. Select mature, plump *Polygonatum yunnanense* seeds, disinfect them by soaking in a 2% sodium hypochlorite solution for 15 minutes, and then rinse them four times with sterile water; subsequently, place the seeds in a compound enzyme solution and soak them at 19℃ for 3 hours. The compound enzyme solution consists of pectinase with an activity of 100,000 u / g and cellulase with an activity of 5,000 u / g, both at 0.05% of the seed weight.

[0038] S2. The enzyme-treated seeds were subjected to variable-temperature cyclic culture, with the cycle conditions being 25℃ for 8 hours and 5℃ for 16 hours, for a total of 20 days. Immediately afterwards, the seeds were subjected to ultrasonic treatment at a power of 200W for 2 minutes. Immediately after treatment, the seeds were immersed in warm water at 33℃ for 8 minutes.

[0039] S3. The seeds treated with S2 were soaked in a compound solution for 18 hours. The compound solution consisted of GA3 100 mg / L, 6-BA 30 mg / L, peptone 10 g / L, and the pH was adjusted to 6.0.

[0040] S4. Sow the soaked seeds in 1 / 2 MS solid medium supplemented with 0.8 g / L humic acid and 2.5 g / L perlite. Culture under conditions of 20°C, 65% humidity, and a 12h light / 12h dark photoperiod (white LED light, 2500 Lux). On day 12 of culture, a phased stress-resistance treatment was performed, adjusting the environment to 20°C and 58% humidity for 2 days, after which the initial culture conditions were restored.

[0041] Comparative Example 4 Using the traditional method: select mature and plump seeds of Polygonatum yunnanense, disinfect them, soak them directly in 400 mg / L GA3 solution for 24 hours, and then sow them in moist sand and cultivate them under variable temperature conditions of 15-25℃.

[0042] Experimental results 100 mature, plump, and uniformly sized *Polygonatum yunnanense* seeds were selected from each group, totaling 500 seeds. All seeds were treated according to the methods specified for their respective groups. The treated seeds were then sown in their designated germination substrates and cultured under uniform, controlled environmental conditions. Germination was recorded on days 3, 5, 7, 10, and 12 after sowing, with the emergence of the radicle from the seed coat as the germination marker. The germination rate for each group was calculated using the following formula: Seed germination rate (%) = (final number of germinated seeds / number of tested seeds) × 100%.

[0043] Example 2 S1. Select mature, plump *Polygonatum yunnanense* seeds, disinfect them by soaking in a 1.5% sodium hypochlorite solution for 20 minutes, then rinse three times with sterile water, each rinse lasting at least one minute. After rinsing, soak the seeds in a compound enzyme solution for 2.5 hours. The compound enzyme solution consists of pectinase with an activity of 100,000 u / g and cellulase with an activity of 5,000 u / g, both at 0.06% of the seed weight, and the treatment temperature is controlled at 18℃.

[0044] S2. The enzyme-treated seeds were subjected to variable-temperature cycling culture, with a daily cycle of 8 hours at 25℃ and 16 hours at 5℃, for 18 days. Immediately afterwards, the seeds were subjected to ultrasonic treatment at 250W for 1.5 minutes. After ultrasonic treatment, the seeds were immediately immersed in 32℃ warm water for 10 minutes.

[0045] S3. Soak the seeds, after soaking in warm water in S2, in the compound solution for 16 hours. The compound solution consists of: gibberellin (GA3) 80 mg / L, cytokinin (6-BA) 40 mg / L, yeast extract 8 g / L, and pH adjusted to 5.9. During the soaking process, observe closely, remove seeds floating on the surface, and transfer them to a single gibberellin (GA3) solution with a concentration of 80 mg / L that does not contain 6-BA or organic nitrogen sources. Continue soaking until the total soaking time reaches 16 hours.

[0046] S4. Sow the soaked seeds (including normally sinking seeds and sorted floating seeds) evenly in the germination substrate; the germination substrate is a composite substrate of humus, river sand and coconut coir mixed in a volume ratio of 3:1:1, which has been autoclaved at 121℃ for 30 minutes before use; place the culture containers after sowing in a controlled environment incubator for cultivation, with the following conditions: temperature 20℃, humidity 65%, photoperiod 12h light / 12h dark (using white LED lights as the light source, with light intensity controlled at around 2600 Lux). On the 10th day after seed sowing and cultivation, a staged stress resistance treatment is carried out, adjusting the incubator environment to temperature 18℃ and humidity 57% for 2 days; after the stress resistance treatment, return to the initial cultivation conditions and continue cultivation.

[0047] Comparative Example 5 S1. Select mature, plump *Polygonatum yunnanense* seeds, disinfect them by soaking in a 1.5% sodium hypochlorite solution for 20 minutes, then rinse them three times with sterile water, each rinse lasting at least one minute. After rinsing, soak the seeds in a compound enzyme solution for 2.5 hours. The compound enzyme solution consists of pectinase with an activity of 100,000 u / g and cellulase with an activity of 5,000 u / g, both at 0.06% of the seed weight, and the treatment temperature is controlled at 18℃.

[0048] S2. The enzyme-treated seeds were subjected to variable-temperature cycling culture, with a daily cycle of 8 hours at 25℃ and 16 hours at 5℃, for 18 days. Immediately afterwards, the seeds were subjected to ultrasonic treatment at 250W for 1.5 minutes. After ultrasonic treatment, the seeds were immediately immersed in warm water at 32℃ for 10 minutes.

[0049] S3. Soak the seeds, after soaking in warm water in S2, in the compound solution for 16 hours. The compound solution consists of: gibberellin (GA3) 80 mg / L, cytokinin (6-BA) 40 mg / L, yeast extract 8 g / L, and pH adjusted to 5.9. During the soaking process, observe closely, remove seeds floating on the surface, and transfer them to a single gibberellin (GA3) solution with a concentration of 80 mg / L that does not contain 6-BA or organic nitrogen sources. Continue soaking until the total soaking time reaches 16 hours.

[0050] S4. Sow the soaked seeds (including normally sinking seeds and sorted floating seeds) evenly in the germination substrate; the germination substrate is a composite substrate of humus, river sand and coconut coir mixed in a volume ratio of 3:1:1, which has been autoclaved at 121℃ for 30 minutes before use; place the culture containers after sowing in a controlled environment incubator for cultivation, with the following conditions: temperature 20℃, humidity 65%, photoperiod 12h light / 12h dark (using white LED lights as the light source, with light intensity controlled at around 2600 Lux). On the 10th day after seed sowing and cultivation, a staged stress resistance treatment is carried out, adjusting the incubator environment to temperature 18℃ and humidity 57% for 2 days; after the stress resistance treatment, return to the initial cultivation conditions and continue cultivation.

[0051] Comparative Example 6 S1. Select mature, plump *Polygonatum yunnanense* seeds, disinfect them by soaking in a 1.5% sodium hypochlorite solution for 20 minutes, then rinse them three times with sterile water, each rinse lasting at least one minute. After rinsing, soak the seeds in a compound enzyme solution for 2.5 hours. The compound enzyme solution consists of pectinase with an activity of 100,000 u / g and cellulase with an activity of 5,000 u / g, both at 0.06% of the seed weight, and the treatment temperature is controlled at 18℃.

[0052] S2. The enzyme-treated seeds were subjected to variable temperature cycling culture, with a daily cycle of 8 hours at 25℃ and 16 hours at 5℃, for 18 days.

[0053] S3. Soak the seeds treated in S2 in the compound solution for 16 hours. The compound solution consists of: 80 mg / L gibberellin (GA3), 40 mg / L cytokinin (6-BA), 8 g / L yeast extract, and pH adjusted to 5.9. During the soaking process, observe closely, remove seeds floating on the surface, and transfer them to a single gibberellin (GA3) solution with a concentration of 80 mg / L that does not contain 6-BA or organic nitrogen sources. Continue soaking until the total soaking time reaches 16 hours.

[0054] S4. Sow the soaked seeds (including normally sinking seeds and sorted floating seeds) evenly in the germination substrate; the germination substrate is a composite substrate of humus, river sand and coconut coir mixed in a volume ratio of 3:1:1, which has been autoclaved at 121℃ for 30 minutes before use; place the culture containers after sowing in a controlled environment incubator for cultivation, with the following conditions: temperature 20℃, humidity 65%, photoperiod 12h light / 12h dark (using white LED lights as the light source, with light intensity controlled at around 2600 Lux). On the 10th day after seed sowing and cultivation, a staged stress resistance treatment is carried out, adjusting the incubator environment to temperature 18℃ and humidity 57% for 2 days; after the stress resistance treatment, return to the initial cultivation conditions and continue cultivation.

[0055] Comparative Example 7 S1. Select mature and plump *Polygonatum yunnanense* seeds, disinfect them by soaking in a 1.5% sodium hypochlorite solution for 20 minutes, and then rinse them three times with sterile water, each rinse lasting at least one minute. After rinsing, soak the seeds in a compound enzyme solution for 2.5 hours; the compound enzyme solution consists of pectinase with an activity of 100,000 u / g and cellulase with an activity of 5,000 u / g, both at 0.06% of the seed weight, and the treatment temperature is controlled at 18℃.

[0056] S2. The enzyme-treated seeds were subjected to variable-temperature cycling culture, with a daily cycle of 8 hours at 25℃ and 16 hours at 5℃, for 18 days. Immediately afterwards, the seeds were subjected to ultrasonic treatment at 250W for 1.5 minutes. After ultrasonic treatment, the seeds were immediately immersed in warm water at 32℃ for 10 minutes.

[0057] S3. Soak the seeds, which have been soaked in warm water in S2, in the compound solution for 16 hours. The compound solution consists of: gibberellin (GA3) 80 mg / L, cytokinin (6-BA) 40 mg / L, yeast extract 8 g / L, and pH adjusted to 5.9.

[0058] S4. The seeds treated in S3 were evenly sown in the germination substrate; the germination substrate was a composite substrate of humus, river sand and coconut coir mixed in a volume ratio of 3:1:1, which was autoclaved at 121℃ for 30 minutes before use; the culture containers after sowing were placed in a controlled environment incubator for cultivation under the following conditions: temperature 20℃, humidity 65%, photoperiod 12h light / 12h dark (using white LED lights as the light source, with light intensity controlled at around 2600 Lux). On the 10th day after seed sowing and cultivation, a staged stress resistance treatment was carried out, adjusting the incubator environment to temperature 18℃ and humidity 57% for 2 days; after the stress resistance treatment, the initial cultivation conditions were restored and cultivation continued.

[0059] Comparative Example 8 Using traditional methods: select mature and plump seeds of Polygonatum yunnanense, disinfect the seeds, soak them in 400 mg / L GA3 for 24 hours, and sow them in moist sand for natural cultivation.

[0060] Experimental results One hundred mature, plump, and uniformly sized *Polygonatum yunnanense* seeds were selected from each group, totaling 500 seeds. These seeds were cultured under their respective conditions, and the germination rate was recorded on days 3, 5, 7, 10, and 12 after sowing. Germination was defined as the radicle breaking through the seed coat. The seed germination rate for each group was finally calculated using the following formula: Seed germination rate (%) = (final number of germinated seeds / number of tested seeds) × 100%.

[0061] In summary, both embodiments achieve the unified effect of "high germination rate, short cycle, and strong seedlings". Compared with the traditional method, the germination rate is increased by more than 1 times, the cycle is shortened by more than 60%, and the transplant survival rate is increased to more than 90%. This solves a series of problems in the background technology, such as "deep dormancy, long cycle, and low efficiency" of Yunnan Polygonatum seed germination and "high cost and poor uniformity" of asexual reproduction.

[0062] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made to it in form and detail without departing from the scope defined by the claims of the present invention.

Claims

1. A method for promoting the germination of Polygonatum yunnanense seeds, characterized in that, Includes the following steps: S1. Select mature and plump seeds of Polygonatum yunnanense and treat them with a compound enzyme solution containing pectinase and cellulase. S2. The seeds treated in S1 were subjected to variable temperature cycling culture and ultrasonic treatment in sequence; S3. Soak the seeds treated in S2 in a compound solution containing gibberellin (GA3), cytokinin (6-BA) and organic nitrogen source. Seeds that float on the surface of the solution during soaking are removed and transferred to a gibberellin solution without 6-BA and organic nitrogen source for further soaking. S4. Sow the soaked seeds in the germination substrate for cultivation.

2. The method for promoting the germination of Polygonatum yunnanense seeds as described in claim 1, characterized in that... In step S1, before the compound enzyme solution treatment, the seeds are first disinfected with a 1-3% sodium hypochlorite solution for 10-20 minutes and then rinsed with sterile water.

3. The method for promoting the germination of Polygonatum yunnanense seeds as described in claim 1, characterized in that... The pectinase activity is 100,000 u / g, and the cellulase activity is 5,000 u / g, with both used at a dosage of 0.03-0.08% of the seed weight.

4. The method for promoting the germination of Polygonatum yunnanense seeds as described in claim 1, characterized in that... In step S2, after ultrasonic treatment, the seeds need to be soaked in warm water at 30-35℃.

5. The method for promoting the germination of Polygonatum yunnanense seeds as described in claim 1, characterized in that... The variable temperature cyclic culture consists of a daily cycle of 8 hours at 25℃ and 16 hours at 5℃, lasting for 15-25 days.

6. The method for promoting the germination of Polygonatum yunnanense seeds as described in claim 1, characterized in that... In the composite solution, the concentration of gibberellin (GA3) is 50-150 mg / L, the concentration of cytokinin (6-BA) is 10-50 mg / L, and the concentration of organic nitrogen source is 5-15 g / L.

7. The method for promoting the germination of Polygonatum yunnanense seeds as described in claim 1, characterized in that... In step S4, a staged stress-resistant treatment is also included, with the following conditions: temperature 15-25℃, humidity 55-60%, lasting for 2 days.

8. The method for promoting the germination of Polygonatum yunnanense seeds as described in claim 1, characterized in that... The germination substrate is any one of the following a) or b): a) 1 / 2MS solid culture medium with 0.5-1 g / L humic acid and 2-3 g / L perlite added; b) a composite substrate obtained by mixing humus, river sand and coconut coir in a volume ratio of 3:1:1 and sterilizing it.

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