Sample analyzer and method for cleaning a pipetting needle

Abstract

This application provides a method for cleaning a sample analyzer and a pipette. The method includes: controlling the dispensing component of the sample analyzer to perform a dispensing operation on a target liquid in a target container; and after the dispensing operation is completed, controlling the cleaning device of the sample analyzer to clean the pipette. The method of controlling the cleaning device to clean the pipette includes: controlling the needle moving mechanism of the dispensing component to move the pipette to the cleaning tank of the cleaning device, and controlling the first liquid supply component of the cleaning device to supply a first cleaning solution to the cleaning tank, so as to clean the pipette at least through the first cleaning solution; after the first cleaning operation is completed, controlling the second liquid supply component of the cleaning device to supply a second cleaning solution to the cleaning tank, so as to clean the pipette at least through the second cleaning solution. Both the first and second cleaning solutions are salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution.

Description

Technical Field

[0001] This application relates to the field of medical device technology, and in particular to a method for cleaning a sample analyzer and a pipette. Background Technology

[0002] Sample analyzers are widely used in clinical testing. In sample analyzers, pipettes are typically used for the quantitative addition of samples or reagents. To reduce the chance of contaminants being carried by the pipette during sample or reagent addition, it is necessary to clean the pipette.

[0003] In some applications, after pipette operation, protein residues, such as proteinase K, may remain on the surface of the pipette. In some nucleic acid detection reactions, proteinase K residue can inhibit the activity of the reacting enzyme, affecting the normal progress of the detection process and consequently impacting the accuracy and reliability of the sample detection results. Summary of the Invention

[0004] The main objective of this application is to provide a cleaning method for a sample analyzer and a pipette, which aims to improve the cleaning effect of protein residues on the pipette and reduce the impact of high concentrations of salt solution residues on the surface of the pipette on the sample detection results, so as to effectively improve the accuracy and reliability of the sample detection results.

[0005] In a first aspect, embodiments of this application provide a sample analyzer, comprising:

[0006] A dispensing component is used to perform a dispensing operation, wherein the dispensing operation is used to aspirate a target liquid from a target container and discharge it into a corresponding reaction container; wherein the dispensing component includes a pipette, a needle moving mechanism, and a power mechanism, the needle moving mechanism is used to drive the pipette to move; the power mechanism is used to provide power for the pipette to aspirate and discharge the target liquid, wherein the target liquid includes at least one of a sample and a reagent;

[0007] A measuring component is used to measure the reaction solution to obtain corresponding detection data, wherein the reaction solution is formed by mixing at least a sample and a reagent;

[0008] The cleaning device includes a cleaning tank and a first liquid supply component and a second liquid supply component. The first liquid supply component is used to supply a first cleaning liquid to the cleaning tank, and the second liquid supply component is used to supply a second cleaning liquid to the cleaning tank.

[0009] Control device, at least for:

[0010] The dispensing component is controlled to perform the dispensing operation on the target liquid in the target container, and after the dispensing operation is completed, the cleaning device is controlled to clean the pipette needle;

[0011] The step of controlling the cleaning device to clean the pipette includes:

[0012] The needle moving mechanism is controlled to move the pipette to the cleaning tank, and the cleaning device is controlled to perform a first cleaning operation on the pipette located in the cleaning tank. The first cleaning operation includes at least controlling the first liquid supply component to supply the first cleaning solution to the cleaning tank, so as to clean the pipette at least by the first cleaning solution.

[0013] After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component to supply the second cleaning solution to the cleaning tank so as to clean the pipette at least by the second cleaning solution. Both the first cleaning solution and the second cleaning solution are salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution.

[0014] Secondly, embodiments of this application provide a sample analyzer, comprising:

[0015] A dispensing component is used to perform a dispensing operation, wherein the dispensing operation is used to aspirate a target liquid from a target container and discharge it into a corresponding reaction container; wherein the dispensing component includes a pipette, a needle moving mechanism, and a power mechanism, the needle moving mechanism is used to drive the pipette to move; the power mechanism is used to provide power for the pipette to aspirate and discharge the target liquid, wherein the target liquid includes at least one of a sample and a reagent;

[0016] A measuring component is used to measure the reaction solution to obtain corresponding detection data, wherein the reaction solution is formed by mixing at least a sample and a reagent;

[0017] The cleaning device includes a cleaning tank and a first liquid supply component and a second liquid supply component. The first liquid supply component is used to supply a first cleaning liquid to the cleaning tank, and the second liquid supply component is used to supply a second cleaning liquid to the cleaning tank.

[0018] Control device, at least for:

[0019] The dispensing component is controlled to perform a first dispensing operation on a first target liquid in a first target container, wherein the first target liquid includes at least proteinase K reagent;

[0020] After the first dispensing operation is completed, the needle moving mechanism is controlled to move the pipette to the cleaning pool, and the cleaning device is controlled to perform a first cleaning operation on the pipette located in the cleaning pool. The first cleaning operation includes controlling the first liquid supply component of the cleaning device to provide a first cleaning solution to the cleaning pool, so as to clean the pipette at least by the first cleaning solution.

[0021] After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes controlling the second liquid supply component of the cleaning device to provide a second cleaning solution to the cleaning tank, so as to clean the pipette at least by the second cleaning solution. The first cleaning solution and the second cleaning solution are both salt solutions, and the concentration of the second cleaning solution is less than that of the pipette.

[0022] After the second cleaning operation is completed, the dispensing component is controlled to perform a second dispensing operation on the second target liquid in the second target container. The second target liquid includes at least DNA polymerase reagent and / or reverse transcriptase reagent.

[0023] Thirdly, embodiments of this application provide a method for cleaning a pipette, applied to a sample analyzer, the method comprising:

[0024] The sample analyzer is controlled to perform a dispensing operation on the target liquid in the target container, and after the dispensing operation is completed, the sample analyzer is controlled to clean the pipette.

[0025] The step of controlling the cleaning device to clean the pipette includes:

[0026] The needle moving mechanism of the dispensing component is controlled to move the pipette needle to the cleaning tank of the cleaning device, and the cleaning device is controlled to perform a first cleaning operation on the pipette needle located in the cleaning tank. The first cleaning operation includes at least controlling the first liquid supply component of the cleaning device to supply a first cleaning solution to the cleaning tank, so as to clean the pipette needle at least by the first cleaning solution.

[0027] After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component of the cleaning device to supply a second cleaning solution to the cleaning tank, so as to clean the pipette at least by the second cleaning solution. Both the first cleaning solution and the second cleaning solution are salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution.

[0028] Fourthly, embodiments of this application provide a method for cleaning a pipette, applied to a sample analyzer, the method comprising:

[0029] The sample analyzer is controlled to perform a first dispensing operation on a first target liquid in a first target container, wherein the first target liquid includes at least proteinase K reagent;

[0030] After the first dispensing operation is completed, the needle moving mechanism of the dispensing component is controlled to move the pipette to the cleaning pool, and the cleaning device of the sample analyzer is controlled to perform a first cleaning operation on the pipette located in the cleaning pool. The first cleaning operation includes controlling the first liquid supply component of the cleaning device to provide a first cleaning solution to the cleaning pool, so as to clean the pipette at least by the first cleaning solution.

[0031] After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes controlling the second liquid supply component of the cleaning device to provide a second cleaning solution to the cleaning tank, so as to clean the pipette at least by the second cleaning solution. The first cleaning solution and the second cleaning solution are both salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution.

[0032] After the second cleaning operation is completed, the dispensing component is controlled to perform a second dispensing operation on the second target liquid in the second target container. The second target liquid includes at least DNA polymerase reagent and / or reverse transcriptase reagent.

[0033] As can be seen from the technical solution provided in this application, after the dispensing component completes the dispensing operation of the target liquid, the cleaning device first cleans the pipette with a first cleaning solution, and after the first cleaning solution is completed, the pipette is further cleaned with a second cleaning solution. Furthermore, since both the second and second cleaning solutions are salt solutions, and the concentration of the second cleaning solution is lower than that of the first cleaning solution, even if there are high concentrations of salt residues on the surface of the pipette after cleaning with the first cleaning solution, they can be diluted with the second cleaning solution. This not only improves the cleaning effect of the pipette but also effectively reduces the impact of the high concentration of residual salt residues on the activity of some enzyme reagents used in subsequent detection processes, ultimately improving the accuracy and reliability of sample detection results.

[0034] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit the disclosure of the embodiments of this application. Attached Figure Description

[0035] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0036] Figure 1 This is a block diagram of the sample analyzer in one embodiment;

[0037] Figure 2 This is a schematic diagram of the structural layout of a sample analyzer in one embodiment;

[0038] Figure 3 This is a schematic diagram of the sample dispensing component of a sample analyzer in one embodiment;

[0039] Figure 4 This is a block diagram of the cleaning device of the sample analyzer in one embodiment.

[0040] Figure 5 and Figure 6 This is a schematic diagram of the liquid circuit structure of two different embodiments of the cleaning device for the sample analyzer in one implementation.

[0041] Figure 7A This is a partial flowchart illustrating the reagent aspiration and cleaning process of the pipette of a sample analyzer during the preparation of nucleic acid extraction reagents in one embodiment.

[0042] Figure 7B This is a partial flowchart illustrating the cleaning process of a sample analyzer's cleaning device for cleaning pipettes in one embodiment.

[0043] Figures 8 to 15 This is a partial flowchart illustrating the cleaning process of a sample analyzer's cleaning device for cleaning pipettes in several different implementations.

[0044] Figures 16 to 18 This is a schematic diagram of the cleaning device of the sample analyzer in several different implementations, showing the cleaning of the pipette and the partial process of liquid aspiration after cleaning the pipette.

[0045] Figure 19 and Figure 20 These are flowcharts showing the steps involved in cleaning the pipette in two different implementation methods. Detailed Implementation

[0046] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0047] In the description of this application, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0048] The flowchart shown in the attached diagram is for illustrative purposes only and does not necessarily include all content and operations / steps, nor does it necessarily have to be performed in the order described. For example, some operations / steps can be broken down, combined, or partially merged, so the actual execution order may change depending on the actual situation.

[0049] The following detailed description of some embodiments of this application is provided in conjunction with the accompanying drawings. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0050] Sample analyzers are widely used in clinical testing. In sample analyzers, pipettes are typically used for the quantitative addition of samples or reagents. To reduce the chance of contaminants being carried by the pipette during sample or reagent addition, it is necessary to clean the pipette.

[0051] In some applications, after pipette operations, protein residues, such as proteinase K, may remain on the surface of the pipette. In certain nucleic acid detection reactions, proteinase K residue can inhibit the activity of the reacting enzymes, affecting the normal progress of the detection process and consequently impacting the accuracy and reliability of the sample test results. To improve the cleaning effect of protein residues, high-concentration salt solutions are typically used to clean the pipette. However, the high concentration of salt solution remaining on the pipette surface can affect the activity of some enzyme reagents used in subsequent detection procedures, further impacting the accuracy and reliability of the sample test results.

[0052] Based on this, this application provides a method for cleaning a sample analyzer and a pipette, which aims to improve the cleaning effect on protein residues on the pipette and reduce the impact of high concentrations of salt solution residues on the surface of the pipette on the sample detection results, so as to effectively improve the accuracy and reliability of the sample detection results.

[0053] Please see Figure 1 This application provides a sample analyzer 100 for analyzing a sample to be tested to obtain corresponding analytical results. The sample analyzer 100 includes, but is not limited to, at least one of the following: a biochemical analyzer, an immunoassay analyzer, a coagulation analyzer, a urine analyzer, and a molecular diagnostic analyzer.

[0054] like Figure 1 As shown, the sample analyzer 100 includes a supply device 100a, a detection device 100b, and a control device 70. The supply device 100a is used to supply samples, reagents, and consumables. For example, the supply device 100a includes a consumable component 10, a sample component 20, and a reagent component 30. The consumable component 10 carries and supplies consumables, the sample component 20 carries and supplies samples, and the reagent component 30 carries and supplies reagents. The detection device 100b is used to prepare a reaction solution or mixture formed by mixing the sample and reagents, and to detect the reaction solution or mixture to obtain corresponding detection data.

[0055] The control device 70 is communicatively connected to the supply device 100a and the detection device 100b, and is used to control the functional modules of the sample analyzer 100 to perform corresponding actions to achieve specific item detection. Each functional module is at least one of the supply device 100a and the detection device 100b. Each functional module can respond to the control command of the control device 70 and perform corresponding actions according to a preset process to achieve a corresponding preset function. For example, a functional module may be a sample component 20, which can respond to the control command of the control device 70 and perform a sample supply operation according to a preset process to transfer the sample to the corresponding sample aspiration position. Another example is a reagent component 30, which can respond to the control command of the control device 70 and perform a reagent supply operation according to a preset process to transfer the reagent to the corresponding reagent aspiration position. Yet another example is a detection device 100b, which can respond to the control command of the control device 70 and perform a corresponding reaction solution preparation operation according to a preset process to mix the sample and reagent to form a reaction solution, and then detect the reaction solution to obtain corresponding detection data.

[0056] Those skilled in the art should understand that, Figure 1 This is merely an example of a sample analyzer 100 and does not constitute a limitation on the sample analyzer 100. The sample analyzer 100 may include components such as... Figure 1 The sample analyzer 100 may include more or fewer components, or combinations of certain components, or different components. It may also include input / output devices, network access devices, etc.

[0057] In some embodiments, the consumable component 10 is provided with at least one consumable carrier position. For example, the consumable carrier device 10 is provided with multiple consumable carrier positions, and the consumables carried in different consumable carrier positions can be the same type of consumable or different types of consumables. The consumables supplied by the consumable component 10 include, but are not limited to, PCR tubes, extraction strips (also known as molecular diagnostic extraction strips), TIP tips, etc.

[0058] like Figure 2 As shown, in some embodiments, the sample component 20 may include a sample delivery module (SDM) and a front-end track. Samples are placed in sample tubes, and a sample rack carrying one or more sample tubes is moved to the corresponding injection position via the front-end track. The sample delivery module then transfers the sample rack located at the injection position to a preset position (such as the aspiration position). In other examples, the sample component 20 may also be a sample tray, which includes multiple sample positions for placing sample tubes. By rotating its tray structure, the sample tray can be moved to the corresponding position, such as the aspiration position where the detection device 100b aspirates the sample.

[0059] In some embodiments, the reagent component 30 is used to provide reagents for reaction with the sample. The reagent component 30 is provided with a plurality of reagent positions 301b, which are used to hold reagent containers. The reagents for reaction with the sample are stored in the reagent containers. The reagents include, but are not limited to, at least one of PCR reagents, IC reagents (internal standard solution), chromogenic reagents, diluents, substrate solutions, enzyme-labeled reagents, magnetic bead reagents, washing solutions, DNA polymerase reagents, and reverse transcriptase reagents. It is understood that the washing solution can be one or more types, and this is not limited thereto.

[0060] In some embodiments, the reagent component 30 can be a disc-shaped structure or a linear structure. For example, the reagent component 30 includes a reagent carrying mechanism 301, which is disc-shaped (also called a reagent tray). The reagent carrying mechanism 301 is provided with multiple reagent positions 301b, which can rotate and drive the reagents carried by the reagent positions 301b to rotate. By rotating the reagent carrying mechanism 301, the reagents can be transferred to the reagent aspiration positions for the dispensing component of the detection device 100b to aspirate the reagents. The reagent carrying mechanism 301 may have an openable and closable reagent tray cover, which allows the user to place or remove reagents into or from the reagent carrying mechanism 301 when the reagent tray cover is opened.

[0061] Optionally, the reagent carrying mechanism 301 may include at least one rotatable reagent track 301a, the reagent track 301a being provided with a plurality of reagent positions 301b for carrying reagents, and the reagent track 301a rotating to drive the reagent containers on its reagent positions 301b to move.

[0062] Optionally, the reagent carrying mechanism 301 includes multiple reagent tracks 301a, each capable of independent rotation. The reagent tracks 301a can rotate and drive the reagent containers they carry to move, thereby rotating the reagent containers to the reagent aspiration position for the dispensing component of the detection device 100b to aspirate the reagents.

[0063] In some embodiments, the reagent component 30 can provide cooling or other functions for the reagent it carries, such as maintaining the temperature between 2 and 16 degrees Celsius to ensure the activity of the reagent. Specifically, the reagent component 30 is used to maintain its internal temperature within the range required by the reagent's instruction manual. To ensure the cooling effect, the reagent component 30 can be a closed structure; for example, the reagent component 30 can be provided with an openable and closable lid to achieve heat preservation. The reagent component 30 can have a detector for detecting the presence or absence of reagents, used to detect whether a reagent container is carried on the reagent position 301b.

[0064] In some embodiments, the detection device 100b includes a reaction component 40, a dispensing component 50, and a measuring component 60. The measuring component 60 is used to detect the reaction solution formed by mixing the sample and reagents to obtain detection data. For example, the measuring component 60 is used to measure the reaction solution within the reaction vessel to obtain sample detection data.

[0065] Optionally, the measuring component 60 includes a photometric mechanism for detecting the luminescence intensity of the reaction liquid to obtain the luminescence intensity of the reaction liquid. Then, using a calibration curve and the luminescence intensity, the concentration of the analyte in the sample can be calculated. It is understood that the detection data acquired by the measuring component 60 can be either the luminescence intensity of the reaction liquid or the concentration of the analyte in the reaction liquid. That is, calculating the concentration of the analyte in the sample using a calibration curve and the luminescence intensity can be performed by either the measuring component 60 or the control device 70; no limitation is imposed here.

[0066] It is understood that, in some embodiments, the measuring component 60 includes an electrical detection mechanism (e.g., an impedance measurement mechanism) or a detection mechanism based on other principles (e.g., an imaging measurement mechanism).

[0067] Dispensing component 50 is used to perform dispensing operations, which are used to aspirate the target liquid from the target container and discharge it into the corresponding reaction container. The target container includes, but is not limited to, reagent bottles and sample tubes, and the target liquid includes at least one of a sample and a reagent.

[0068] The reaction component 40 includes a support mechanism 401, which has at least one reaction position 4011 for placing a reaction container. The reaction container receives samples and reagents and provides a mixing area for them. The reaction container includes, but is not limited to, reaction cups and reaction tubes. For example, the support mechanism 401 is arranged in a disc-shaped assembly, also called a reaction tray. The support mechanism 401 has one or more reaction positions 4011 for placing the reaction container. The reaction tray can incubate the reaction liquid in the reaction container and can rotate to rotate the reaction container placed in the reaction position 4011, realizing the scheduling of the reaction container within the reaction tray in a preset area, for example, scheduling the reaction container located in the reaction position 4011 to the position for reagent sample addition.

[0069] Optionally, the sample analyzer 100 is provided with a sample aspiration position and a reagent aspiration position. During the preparation of the reaction solution for some test items, the dispensing component 50 aspirates the sample supplied by the sample component 20 and the reagent supplied by the reagent component 30, respectively, and dispenses the aspirated sample and reagent into the reaction container placed in the preset operating position, so that the sample and reagent mix in the reaction container to form a reaction solution. Optionally, the reaction component 40 is also used to incubate the reaction solution formed by mixing the sample and reagent.

[0070] Optionally, the detection device 100b is further provided with a nucleic acid extraction component 80 for extracting nucleic acid (DNA or RNA) from the sample to be tested, and a consumable component 10 for providing consumables required during the detection process, including but not limited to at least one of PCR tubes, extraction strips (also known as molecular diagnostic extraction strips), and TIP pipette tips.

[0071] For example, in the preparation of the reaction solution for a nucleic acid detection test, the dispensing component 50 draws the corresponding reagent (such as lysis buffer, magnetic bead reagent, IC reagent or proteinase K reagent) from the reagent component 30 to prepare a nucleic acid extraction reagent. The nucleic acid extraction component 80 uses the nucleic acid extraction reagent to extract nucleic acid from the sample to be tested.

[0072] The detection device 100b uses the nucleic acid extraction component 80 to extract nucleic acid from the sample to be tested. A first TIP pipette supplied by the consumable component 10 draws reagent from the reagent component 30 and transfers the drawn reagent to a reaction container (e.g., a PCR tube) supplied by the consumable component 10. Alternatively, the dispensing component 50 draws reagent (e.g., DNA polymerase reagent, reverse transcriptase reagent) from the reagent component 30 and transfers the drawn reagent to a reaction container (e.g., a PCR tube) supplied by the consumable component 10. A second TIP pipette supplied by the consumable component 10 then transfers the nucleic acid (e.g., target sequence DNA) extracted by the nucleic acid extraction component 80 into the reaction container containing the reagent to prepare the sample solution to be tested (also called the reaction solution). After obtaining the sample solution to be tested, the control device 70 controls the measurement component 60 to perform polymerase chain reaction detection (e.g., nucleic acid amplification and real-time fluorescence detection) on the sample solution to obtain detection data.

[0073] Please see Figure 2 and Figure 3 In some embodiments, the dispensing component 50 includes a sample dispensing component 50a, which performs a sample dispensing operation. Specifically, the sample dispensing component 50a aspirates the sample supplied by the sample component 20 and transfers the sample to a preset location. For example, the sample component 20 carries a sample tube containing the sample to be tested, and the sample dispensing component 50a aspirates the sample to be tested from the sample tube carried by the sample component 20 and discharges the sample into the reaction vessel to be added.

[0074] Optionally, the sample dispensing component 50a includes a dispensing needle 501 and a first needle moving mechanism 502. The first needle moving mechanism 502 supports the dispensing needle 501 and drives or moves the dispensing needle 501. For example, the dispensing needle 501 can move in two or three dimensions in space through the two-dimensional or three-dimensional first needle moving mechanism 502, so that the dispensing needle 501 can move to pick up the sample carried by the sample component 20.

[0075] Optionally, the sample dispensing component 50a further includes a first power mechanism 503, which provides power for the sampling needle 501 to perform sample aspiration and / or dispensing, thereby completing the sample aspiration and / or dispensing. For example, taking the sampling needle 501 performing sample aspiration as an example, the sampling needle 501 moves under the drive of the first needle moving mechanism 502 to the sample tube containing the sample to be tested, and under the drive of the first power mechanism 503, aspirates the sample to be tested and delivers the sample to be tested to the reaction container located in the reaction position of the reaction component 40, so that the sample to be tested aspirated by the sample dispensing component 50a is mixed with the reagent provided by the reagent component 30 in the reaction container.

[0076] like Figure 3As shown, in some embodiments, the first needle moving mechanism 502 includes a support frame 5021, which is fixed to a support rod 5022. The support rod 5022 is vertically movable and rotatable, and the support frame 5021 moves vertically and rotates horizontally under the drive of the support rod 5022. The sample dispensing needle 501 is mounted on the support frame 5021 and can reach the target position under the drive of the support frame 5021. Exemplarily, the first needle moving mechanism 502 also includes a driver 5023 for driving the support rod 5022 to move, such as a stepper motor, but is not limited to this. Optionally, the sample dispensing needle 501 is detachably connected to the first needle moving mechanism 502 or fixedly connected.

[0077] Optionally, the first power mechanism 503 includes a tubing 5031 and a power assembly 5033. The tubing 5031 is used to transport a fluid medium. One end of the tubing 5031 is connected to a sampling needle 501, and the other end is connected to the power assembly 5033, so that the flow direction of the fluid medium in the tubing 5031 can be changed under the action of the power assembly 5033, allowing the sampling needle 501 to transfer samples and / or reagents. The power assembly 5033 includes, but is not limited to, a syringe, a pump, and a valve body disposed on the tubing 5031.

[0078] like Figure 2 As shown, in some embodiments, the dispensing component 50 further includes a reagent dispensing component 50b, which is used to perform a reagent dispensing operation. That is, the reagent dispensing component 50b is used to draw the reagent supplied by the reagent component 30 and transfer the reagent to a preset position. For example, the reagent component 30 carries a reagent container 90 containing reagents, and the reagent dispensing component 50b draws the reagent from the reagent container 90 carried by the reagent component 30 and discharges the reagent into the reaction vessel to be added with the reagent.

[0079] In some embodiments, the reagent dispensing component 50b may include a reagent needle, a second needle moving mechanism, and a second power mechanism. The reagent needle moves in two or three dimensions in space via the two-dimensional or three-dimensional second needle moving mechanism, thereby allowing the reagent needle to move and cooperate with the second power mechanism to draw the reagent carried by the reagent carrying mechanism 301, move to the reaction container to be added, and discharge the reagent into the reaction container.

[0080] In some embodiments, the second needle moving mechanism and the first needle moving mechanism 502 have the same structure, and / or the second power mechanism and the first power mechanism 503 have the same structure, which will not be described in detail here.

[0081] In some embodiments, the reagent dispensing component 50b adds reagents not via a reagent needle, but through a dedicated tubing to add the reagent from the reagent tube into the reaction vessel. In these embodiments, only the sample dispensing needle 501 is used, without a reagent needle.

[0082] It is understandable that, depending on the different bodily fluids being tested and the specific test items, different methods of adding samples and reagents may be used. For example, both samples and reagents can be added using the sampling needle 501, or samples can be added using the sampling needle 501 and reagents using the reagent needle, or only samples can be added using the sampling needle 501 and reagents can be added using other methods. That is, the sample dispensing component 50a of the dispensing unit 50 is used for both sample and reagent transfer; or the sample dispensing component 50a is used for sample transfer and the reagent dispensing component 50b is used for reagent transfer; or the sample dispensing component 50a is used for sample transfer, and the reagent is added to the reaction vessel via a dedicated tubing connected to the reagent container 90. Therefore, the sampling needle 501 and / or the reagent needle are also called pipettes, meaning that a pipette includes at least one of the sampling needle 501 and the reagent needle.

[0083] As described above, the dispensing component 50 includes a pipette (e.g., a sample dispensing needle 501, a reagent needle), a needle movement mechanism (e.g., a first needle movement mechanism, a second needle movement mechanism), and a power mechanism (e.g., a first power mechanism, a second power mechanism). The pipette is used to transfer samples and / or reagents. The needle movement mechanism is used to drive or propel the pipette. The power mechanism is used to provide power for the pipette to aspirate and dispense samples and / or reagents.

[0084] like Figure 1 As shown, in some embodiments, the sample analyzer 100 further includes a cleaning device 90 for providing cleaning fluid to clean target components, including but not limited to pipettes (e.g., sample needle 101, reagent needle). For example, the cleaning device 90 can be used to clean the inner and / or outer walls of the pipette.

[0085] Please see Figure 4 In some embodiments, the cleaning device 90 is provided with a cleaning tank 91 and a first liquid supply component 92 and a second liquid supply component 93. The first liquid supply component 92 is used to provide a first cleaning liquid to the cleaning tank 91 to clean the target component located in the cleaning tank 91. The second liquid supply component 93 is used to provide a second cleaning liquid to the cleaning tank 91 to clean the target component located in the cleaning tank 91.

[0086] Optionally, the first cleaning solution and the second cleaning solution can be the same solution with different concentrations, or they can be different solutions with different concentrations. For example, both the first and second cleaning solutions can be salt solutions, with the concentration of the first cleaning solution being greater than that of the second cleaning solution. Another example is that the first cleaning solution is a salt solution, and the second cleaning solution is water.

[0087] It is understood that there can be one or more cleaning tanks 91, and the first liquid supply component 92 and the second liquid supply component 93 can be used to provide the corresponding cleaning liquid to the same cleaning tank, or to provide the corresponding cleaning liquid to different cleaning tanks.

[0088] Please see Figure 4 and Figure 5 In some embodiments, the cleaning tank 91 includes a first cleaning tank 911 and a second cleaning tank 912. The first cleaning tank 911 has a first cleaning chamber 9110 with a first opening 9111, and is provided with a first inlet 9112 and a first outlet 9113. A first supply component 92 is connected to the first inlet 9112 to supply a first cleaning solution to the first cleaning tank 911 through the first inlet 9112. The second cleaning tank 912 has a second cleaning chamber 9120 with a second opening 9121, and is provided with a second inlet 9122 and a second outlet 9123. A second supply component 93 is connected to the second inlet 9122 to supply a second cleaning solution to the second cleaning tank 912 through the second inlet 9122.

[0089] Optionally, there may be at least one first liquid inlet 9112. For example, there may be multiple first liquid inlets 9112 distributed on the sidewalls of the first cleaning tank 911 at different heights, or they may be distributed circumferentially on the sidewalls of the first cleaning tank 911 at the same height. This is not limited here.

[0090] Optionally, there may be at least one second liquid inlet 9122. For example, there may be multiple second liquid inlets 9122 distributed on the sidewalls of the second cleaning tank 912 at different heights, or they may be distributed circumferentially on the sidewalls of the second cleaning tank 912 at the same height. This is not limited here.

[0091] It is understood that the first cleaning tank 911 and the second cleaning tank 912 can be connected to each other, can be integrated, or can be separated. As long as the first cleaning tank 911 forms the first cleaning chamber 9110 and the second cleaning tank 912 forms the second cleaning chamber 9120, the first liquid supply component 92 provides the first cleaning liquid to the first cleaning chamber 9110 of the first cleaning tank 911, and the second liquid supply component 93 provides the second cleaning liquid to the second cleaning chamber 9120 of the second cleaning tank 912. The target component (such as a pipette) located in the first cleaning chamber 9110 is cleaned with the first cleaning liquid in the first cleaning chamber 9110, and the target component (such as a pipette) located in the second cleaning chamber 9120 is cleaned with the second cleaning liquid in the second cleaning chamber 9120.

[0092] like Figure 5As shown, in some embodiments, the first liquid supply component 92 includes a first pipeline 924 connected to the first liquid inlet 9112, a first switch component 923 disposed on the first pipeline 924, a first cleaning fluid storage component 922, and a first liquid supply power component 921. The first cleaning fluid storage component 922 is used to store the first cleaning fluid, the first switch component 923 is used to control the opening and closing of the liquid passage between the first cleaning fluid storage component 922 and the first liquid inlet 9112, and the first liquid supply power component 921 is used to provide power for the flow of liquid in the first pipeline 924.

[0093] When the first liquid supply component 92 needs to supply the first cleaning fluid to the first liquid inlet 9112, the first switch component 923 can be switched to the conducting state to connect the liquid path between the first cleaning fluid storage component 922 and the first liquid inlet 9112, and control the first liquid supply power component 921 to provide power so that the first cleaning fluid in the first cleaning fluid storage component 922 flows to the first liquid inlet 9112, and flows to the first cleaning chamber 9110 through the first liquid inlet 9112.

[0094] Conversely, if the first liquid supply component 92 is not required to supply the first cleaning fluid to the first liquid inlet 9112, the first switch component 923 can be switched to the off state to shut off the liquid path between the first cleaning fluid storage component 922 and the first liquid inlet 9112, and the first liquid supply power component 921 can be turned off.

[0095] like Figure 5 As shown, in some embodiments, the second liquid supply component 93 includes a second pipeline 934 connected to the second liquid inlet 9122, a second switch component 933 disposed on the third pipeline 934, a second cleaning fluid storage component 932, and a second liquid supply power component 931. The second cleaning fluid storage component 932 is used to store the second cleaning fluid, the second switch component 933 is used to control the opening and closing of the liquid passage between the second cleaning fluid storage component 932 and the second liquid inlet 9122, and the second liquid supply power component 931 is used to provide power for the flow of liquid in the second pipeline 934.

[0096] When the second liquid supply component 93 needs to supply the second cleaning fluid to the second liquid inlet 9122, the second switch component 933 can be switched to the on state to connect the liquid path between the second cleaning fluid storage component 932 and the second liquid inlet 9122, and control the second liquid supply power component 931 to provide power so that the second cleaning fluid in the second cleaning fluid storage component 932 flows to the second liquid inlet 9122, and flows to the second cleaning chamber 9120 through the second liquid inlet 9122.

[0097] Conversely, if the second liquid supply component 93 is not required to supply the second cleaning fluid to the second liquid inlet 9122, the second switch component 933 can be switched to the off state to shut off the liquid path between the second cleaning fluid storage component 932 and the second liquid inlet 9122, and the second liquid supply power component 931 can be turned off.

[0098] It is understood that the first switching component 923 and the second switching component 933 include, but are not limited to, solenoid valves, and the first liquid supply power component 921 and the second liquid supply power component 923 include, but are not limited to, syringes and power pumps.

[0099] like Figure 4 and Figure 5 As shown, in some embodiments, the cleaning device 90 further forms an overflow pool 95 having a third opening 951 and a third drain outlet 952. The third opening 951 is disposed at the top of the overflow pool 95, and the third drain outlet 952 is disposed at the side wall or bottom of the overflow pool 95. Liquid overflowing from the first cleaning pool 911 and / or the second cleaning pool 912 can enter the overflow pool 95 through the third opening 951 and be discharged from the overflow pool 95 through the third drain outlet 952.

[0100] like Figure 4 and Figure 5 As shown, in some embodiments, the cleaning device 90 further includes a waste liquid treatment component 94 for performing waste liquid treatment operations to discharge at least a portion of the liquid in the first cleaning tank 911, and / or, at least a portion of the liquid in the second cleaning tank 912, and / or, at least a portion of the liquid in the overflow tank 95, to the overflow tank 95.

[0101] For example, waste liquid treatment component 94 is connected to a first drain port 9113 to discharge at least a portion of the liquid in the first cleaning tank 911 during waste liquid treatment operation. As another example, waste liquid treatment component 94 is connected to a second drain port 9123 to discharge at least a portion of the liquid in the second cleaning tank 912 during waste liquid treatment operation. Yet another example, waste liquid treatment component 94 is connected to a third drain port 952 to discharge at least a portion of the liquid in the overflow tank 95 during waste liquid treatment operation.

[0102] Optionally, the waste liquid treatment component 94 includes a waste liquid tank 942, a drainage power assembly 941, and a third switch component 943. The waste liquid tank 942 has a receiving cavity and is provided with a first connecting hole and a second connecting hole communicating with the receiving cavity. The drainage power assembly 941 is connected to the waste liquid tank 942 through the first connecting hole. The second connecting hole is connected to the corresponding target drainage port through a pipe. The third switch component 943 is provided on the pipe to control the opening and closing of the corresponding liquid flow channel. The target drainage port includes at least one of the first drainage port 9113, the second drainage port 9123, and the third drainage port 852.

[0103] During the pipette cleaning process, the discharge power component 941 of the waste liquid treatment component 94 operates to generate negative pressure in the waste liquid reservoir 942. Under the action of negative pressure, the waste liquid generated during the pipette cleaning process is discharged into the waste liquid reservoir 942 through the corresponding discharge port. By recovering the waste liquid generated during the cleaning process into the waste liquid reservoir 942 through negative pressure, the probability of the waste liquid generated during the cleaning process re-contaminating the pipette can be effectively reduced, thereby making the cleaning effect of the pipette better.

[0104] Furthermore, during the operation of the drain power assembly 941, a negative pressure can be formed at the corresponding drain ports (e.g., the first drain port 9113, the second drain port 9123, and the third drain port 852), thereby creating a suction effect and enabling the pipette to be dried.

[0105] like Figure 6 As shown, in some embodiments, as described above Figure 5 The cleaning tank 91 in this embodiment differs in that it includes a first cleaning tank 911, which forms a first cleaning chamber 9110 with a first inlet 9112, a third inlet 9114, and a first outlet 9113. A first liquid supply component 92 is connected to the first inlet and supplies a first cleaning solution to the first cleaning tank 911 through the first inlet 9112, so that the target component (e.g., a pipette) can be cleaned within the first cleaning chamber 9110 of the cleaning tank 91 using the first cleaning solution. A second liquid supply component 93 is connected to the third inlet 9114 and supplies a second cleaning solution to the cleaning tank 91 through the third inlet 9114, so that the target component (e.g., a pipette) can be cleaned within the first cleaning chamber 9110 of the cleaning tank 91 using the second cleaning solution.

[0106] Waste liquid treatment component 94 is connected to first drain port 9113 to discharge at least a portion of the liquid in first cleaning tank 911 during waste liquid treatment operation.

[0107] In some embodiments, the sample analyzer 100 also includes a display device for displaying information. For example, it may display sample testing data, or it may display status information corresponding to at least one of the sample, reagents, and consumables during the sample testing process. The display device may be a touch screen, liquid crystal display, LED display, or OLED display, etc.

[0108] It is understood that the sample analyzer 100 may have an integrated display device, or the sample analyzer 100 may be connected to a computer device (e.g., a computer) to display information through the computer device's display device (e.g., a screen), or the sample analyzer 100 may be communicatively connected to a display device and display information through the display device.

[0109] like Figure 1 As shown, in some embodiments, the control device 70 may be one or more, and the control device 70 may be provided on the supply device 100a and the detection device 100b, or it may be provided independently of the supply device 100a and the detection device 100b, which is not limited here.

[0110] Optionally, the control device 70 includes at least a processor 701, a memory 702, a communication interface (not shown), and an I / O interface (not shown). The processor 701, memory 702, communication interface, and I / O interface communicate via a bus. The processor 701 can be a Central Processing Unit (CPU), but it can also be other general-purpose processors, digital signal processors (DSPs), application-specific integrated circuits (ASICs), field-programmable gate arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor can be a microprocessor or any conventional processor.

[0111] The memory 702 contains various computer programs, such as the operating system and application programs, for the processor 701 to execute, as well as the data required to execute these programs. During the analysis of the sample under test, any data requiring local storage can be stored in the memory 702. The I / O interface includes, but is not limited to, serial interfaces such as USB, IEEE 1394, or RS-232C; parallel interfaces such as SCSI, IDE, or IEEE 1284; and analog signal interfaces composed of D / A converters and converters. Input components are connected to the I / O interface, allowing users to directly input data to the control device 70. These input components include, but are not limited to, a keyboard, mouse, touchscreen, or control buttons. The display component can communicate with the control device 70 through the I / O interface to provide relevant information prompts. The communication interface can be any known communication protocol. The communication interface can communicate with the outside world via a network, and the control device 70 can transmit data with any component connected through this network using a preset communication protocol.

[0112] In some embodiments, the control device 70 is at least configured to perform the following operations: specifically, the processor 701 of the control device 70 calls a computer program stored in the memory 702 to perform the following operations:

[0113] The dispensing unit 50 is controlled to perform a dispensing operation on the target liquid in the target container, and after the dispensing operation is completed, the cleaning device 90 is controlled to clean the pipette. The target liquid includes samples and / or reagents.

[0114] The cleaning control device 90 cleans the pipette needles, including:

[0115] The control needle moving mechanism moves the pipette to the cleaning tank and controls the cleaning device 90 to perform a first cleaning operation on the pipette located in the cleaning tank. The first cleaning operation includes at least controlling the first liquid supply component 92 to supply a first cleaning solution to the cleaning tank so as to clean the pipette at least through the first cleaning solution. After the first cleaning operation is completed, the control cleaning device 90 performs a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component 93 to supply a second cleaning solution to the cleaning tank so as to clean the pipette at least through the second cleaning solution.

[0116] Optionally, both the first and second cleaning solutions are salt solutions, and the concentration of the second cleaning solution is lower than the concentration of the first cleaning solution. Optionally, the first cleaning solution is a salt solution, and the second cleaning solution is an aqueous solution.

[0117] Please see Figure 7AFor example, taking nucleic acid testing as an example, nucleic acid testing typically involves a nucleic acid extraction process and a PCR amplification process. First, nucleic acids are extracted from the sample to be tested through the nucleic acid extraction process, and then the nucleic acids are amplified through the PCR amplification process. Finally, the amplified sample is tested using polymerase chain reaction (PCR) using a detection device to obtain test data.

[0118] Nucleic acid extraction requires nucleic acid extraction reagents, while PCR amplification requires DNA polymerase and reverse transcriptase reagents. Nucleic acid extraction reagents are typically dispensed from multiple first target containers via a dispensing unit 50, allowing the reagents to mix and form the nucleic acid extraction reagent mixture. These target reagents include, but are not limited to, lysis buffer, magnetic beads, IC reagents, and proteinase K reagents.

[0119] Furthermore, since the residue of proteinase K reagent has a significant impact on subsequent PCR amplification procedures, at least after the proteinase K reagent is aspirated and discharged by the dispensing component 50 (e.g., reagent dispensing component 50b), or after the solution containing proteinase K reagent is discharged, the pipette (e.g., reagent needle) of the dispensing component 50 (e.g., reagent dispensing component 50b) needs to be cleaned with a high-concentration salt solution. However, after cleaning the pipette with a high-concentration salt solution, some high-concentration salt solution may remain on the surface of the pipette. Since the high-concentration salt on the surface of the pipette can affect the enzyme activity of DNA polymerase and reverse transcriptase in the PCR amplification process, it will affect the yield and quality of the corresponding products in the PCR amplification, and ultimately affect the accuracy and reliability of the detection results.

[0120] Based on this, at least after the dispensing component 50 completes the dispensing operation of the proteinase K reagent in the first target container, the control device 70 controls the cleaning device 90 to first clean the pipette with a first cleaning solution. After the first cleaning solution is completed, the pipette is further cleaned with a second cleaning solution. Furthermore, since both the second and third cleaning solutions are salt solutions, and the concentration of the second cleaning solution is lower than that of the first cleaning solution, even if there are high concentrations of salt residues on the surface of the pipette after cleaning with the first cleaning solution, they can be diluted with the second cleaning solution. This not only improves the cleaning effect of the pipette but also effectively reduces the impact of the high concentration of residual salts on the activity of some enzyme reagents used in subsequent detection processes, ultimately improving the accuracy and reliability of sample detection results.

[0121] It is understood that the cleaning device 90 can perform the first cleaning operation and the second cleaning operation on the pipette in the same cleaning tank, or it can perform the first cleaning operation and the second cleaning operation in different first cleaning tanks and second cleaning tanks respectively, without any limitation.

[0122] In some embodiments, the cleaning tank includes a first cleaning tank 911 and a second cleaning tank 912. A first liquid supply component 92 is used to supply a first cleaning solution to the first cleaning tank 911, and a second liquid supply component 93 is used to supply a second cleaning solution to the second cleaning tank 912. The cleaning device 90 is controlled to clean the pipette, including: controlling a needle moving mechanism to move the pipette to the first cleaning tank 911, and controlling the cleaning device 90 to perform a first cleaning operation on the pipette located in the first cleaning tank 911. The first cleaning operation includes at least controlling the first liquid supply component 92 to supply the first cleaning solution to the first cleaning tank 911, so as to clean the pipette located in the first cleaning tank 911 at least with the first cleaning solution; after the first cleaning operation is completed, controlling the needle moving mechanism to move the pipette to the second cleaning tank 912, and controlling the cleaning device 90 to perform a second cleaning operation on the pipette located in the second cleaning tank 912. The second cleaning operation includes at least controlling the second liquid supply component 93 to supply the second cleaning solution to the second cleaning tank 912, so as to clean the pipette located in the second cleaning tank 912 at least with the second cleaning solution.

[0123] Optionally, the control device 70 is further configured to: after the second cleaning operation is completed, control the waste liquid treatment component 94 to perform waste liquid treatment operation on the solution in the second cleaning tank 912 through the second drain port 9123, wherein the waste liquid treatment operation discharges at least a portion of the solution in the second cleaning tank 912 through the second drain port 9123 into the second cleaning tank 912.

[0124] like Figure 7A and 7B As shown, the following example illustrates how, during the preparation of nucleic acid extraction reagents, the cleaning device 90 cleans the pipette used for dispensing proteinase K reagent or a solution containing proteinase K reagent in two different cleaning pools with a first cleaning solution and a second cleaning solution.

[0125] like Figure 7A As shown, during the preparation of nucleic acid extraction reagents, the dispensing unit 50 first performs a lysis buffer aspiration operation from the first target container storing the lysis buffer to aspirate the corresponding amount of lysis buffer.

[0126] After the lysis buffer aspiration operation is completed, an air isolation aspiration operation is performed to form an air isolation section at the pipette. Then, the outer wall of the pipette is cleaned by the cleaning device 90. After the outer wall of the pipette is cleaned, the pipette performs a magnetic bead reagent aspiration operation from the first target container storing magnetic bead reagent to aspirate the corresponding amount of magnetic bead reagent.

[0127] After completing the magnetic bead reagent aspiration operation, an air isolation aspiration operation is performed again to form an air isolation section at the pipette. Then, the outer wall of the pipette is cleaned by the cleaning device 90. After the outer wall of the pipette is cleaned, the pipette performs an IC reagent aspiration operation from the first target container storing IC reagent to aspirate the corresponding amount of IC reagent.

[0128] After completing the IC reagent aspiration operation, perform the proteinase K reagent aspiration operation from the first target container storing the proteinase K reagent to aspirate the corresponding amount of proteinase K reagent.

[0129] After completing the proteinase K reagent aspiration operation, discharge the proteinase K reagent, IC reagent, magnetic bead reagent and lysis buffer from the pipette into the target reaction vessel so that the proteinase K reagent, IC reagent, magnetic bead reagent and lysis buffer are mixed to form nucleic acid extraction reagent.

[0130] After the dispensing unit 50 completes the discharge of proteinase K reagent or solution containing proteinase K reagent into the target reaction vessel or target container, the cleaning device 90 first cleans the pipette with a first cleaning solution, and then cleans the pipette with a second cleaning solution.

[0131] like Figure 7B As shown, the cleaning device 90 can clean the pipette first with a first cleaning solution and then with a second cleaning solution in the following manner:

[0132] The pipette used for dispensing is moved to the first cleaning tank 911 by the needle moving mechanism. The first liquid supply component 92 is controlled to supply the first cleaning solution to the first cleaning tank 911, so as to clean at least the outer wall of the pipette located in the first cleaning tank 911 and the inner wall of the pipette by the first cleaning solution retained in the first cleaning tank 911. After the first liquid supply component 92 supplies the first cleaning solution to the first cleaning tank 911 for a preset time, it is determined that the first cleaning operation of the pipette in the first cleaning tank 911 is completed. Then, the pipette is moved to the second cleaning tank 912 by the needle moving mechanism, and the second liquid supply component 93 is controlled to supply the second cleaning solution to the second cleaning tank 912, so as to clean the pipette located in the second cleaning tank 912 by the second cleaning solution.

[0133] Optionally, after completing the IC reagent aspiration operation, an air isolation aspiration operation is performed again to form an air isolation segment at the pipette, and then the proteinase K reagent aspiration operation is performed from the first target container storing the proteinase K reagent.

[0134] Please see Figure 8In some embodiments, the first cleaning tank 911 is provided with a first inlet 9112 and a first outlet 9113. The cleaning device 90 also includes a waste liquid treatment component 94, which is connected to the first outlet 9113, and a first supply component 92 is connected to the first inlet 9112. The cleaning device 90 is controlled to perform a first cleaning operation on the pipette located in the first cleaning tank 911, including: controlling the first supply component 92 to supply the first cleaning liquid to the first cleaning tank 911 through the first inlet 9112, and recording the supply time of the first cleaning liquid; when the supply time of the first cleaning liquid reaches a first preset time, controlling the waste liquid treatment component 94 to perform a first waste liquid treatment operation on the solution in the first cleaning tank 911 through the first outlet 9113, wherein the first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first outlet 9113.

[0135] Optionally, before the waste liquid treatment component 94 performs the first waste liquid treatment operation, the control device 70 is further configured to: control the first liquid supply component 92 to stop supplying the first cleaning liquid to the first cleaning tank 911.

[0136] Optionally, during the cleaning of the pipette in the first cleaning tank 911, the first drain port 9113 is closed, and the solution overflowing from the first cleaning tank 911 is discharged through the third drain port 952 of the overflow tank 95.

[0137] For example, the first liquid supply component 92 can supply the first cleaning fluid to the first cleaning tank 911 through the first liquid inlet 9112 for a duration that can be set as needed, such as 0.2s, 0.3s, 0.5s, 1s, etc. In order to improve the cleaning effect of the pipette in the first cleaning tank 911, the waste liquid in the first cleaning tank 911 is discharged through the waste liquid treatment component 94 after the pipette cleaning is completed.

[0138] Optionally, after the first cleaning tank 911 is drained, the waste liquid treatment component 94 is kept running for a preset time, so that the negative pressure formed by the waste liquid treatment component 94 at the first drain port 9113 can be used to dry the pipette located in the first cleaning tank 911.

[0139] After the waste liquid treatment component 94 has been open for a preset period of time, the control needle moving mechanism drives the pipette to move to the second cleaning tank 912, and controls the second liquid supply component 93 to supply the second cleaning liquid to the second cleaning tank 912, so as to clean the pipette located in the second cleaning tank 912 with the second cleaning liquid.

[0140] Please see Figure 9In some embodiments, the first cleaning tank 911 is provided with a first inlet 9112, a third inlet 9114, and a first outlet 9113. The cleaning device 90 also includes a waste liquid treatment component 94, which is connected to the first outlet 9113. A first liquid supply component 92 is connected to the first inlet 9112, and a second liquid supply component 93 is connected to the third inlet 9114. Controlling the cleaning device 90 to perform a first cleaning operation on the pipette located in the first cleaning tank 911 includes controlling the first liquid supply component 92 to supply a first cleaning solution to the first cleaning tank 911 through the first inlet 9112. The system records the duration of the first cleaning fluid supply. When the duration of the first cleaning fluid supply reaches the first preset duration, the system controls the second fluid supply component 93 to supply the second cleaning fluid to the first cleaning tank 911 through the first inlet 9113, and records the duration of the second cleaning fluid supply. When the duration of the second cleaning fluid supply reaches the second preset duration, the system controls the waste liquid treatment component 94 to perform a first waste liquid treatment operation on the solution in the first cleaning tank 911 through the first outlet 9113. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first outlet 9113 into the first cleaning tank 911.

[0141] In some embodiments, in order to improve the cleaning effect of the pipette and reduce the residue of the first cleaning solution, before the waste liquid treatment component 94 performs the first waste liquid treatment operation, the control device 70 is further configured to: control the first liquid supply component 92 to stop supplying the first cleaning solution to the first cleaning tank 911 and control the second liquid supply component 93 to supply the second cleaning solution to the first cleaning tank 911 through the second inlet when the liquid supply time of the second cleaning solution reaches the second preset time; and control the second liquid supply component 93 to stop supplying the second cleaning solution to the first cleaning tank 911 when the liquid supply time of the second cleaning solution reaches the second preset time.

[0142] like Figure 9 As shown, in this embodiment, the pipette is first cleaned in the first cleaning tank 911 with the first cleaning solution. After the pipette is cleaned with the first cleaning solution, it is then cleaned in the first cleaning tank 911 with the second cleaning solution. After the pipette is cleaned with the second cleaning solution, the waste liquid in the first cleaning tank 911 is discharged through the waste liquid treatment component 94.

[0143] Optionally, after the first cleaning tank 911 is drained, the waste liquid treatment component 94 is kept running for a preset time, so that the negative pressure formed by the waste liquid treatment component 94 at the first drain port 9113 can be used to dry the pipette located in the first cleaning tank 911.

[0144] After the waste liquid treatment component 94 has been open for a preset period of time, the control needle moving mechanism drives the pipette to move to the second cleaning tank 912, and controls the second liquid supply component 93 to supply the second cleaning liquid to the second cleaning tank 912, so as to clean the pipette located in the second cleaning tank 912 with the second cleaning liquid.

[0145] Please see Figure 10 In some embodiments, the first cleaning tank 911 is provided with a first inlet 9112, a third inlet 9114, and a first outlet 9113. The cleaning device 90 also includes a waste liquid treatment component 94, which is connected to the first outlet 9113. A first supply component 92 is connected to the first inlet 9112, and a second supply component 93 is connected to the third inlet 9114. The cleaning device 90 is controlled to perform a first cleaning operation on the pipette located in the first cleaning tank 911, including: controlling the first supply component 92 to supply the first cleaning liquid to the first cleaning tank 911 through the first inlet 9112, and recording the supply time of the first cleaning liquid; when the supply time of the first cleaning liquid reaches a first preset time, controlling the waste liquid treatment component 94 to supply the waste liquid to the pipette located in the first cleaning tank 9113 through the first outlet 9113. The liquid outlet 9113 performs a first waste liquid treatment operation on the solution in the first cleaning tank 911. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first drain outlet 9113. After the first waste liquid treatment operation is completed, the second liquid supply component 93 is controlled to supply the second cleaning liquid to the first cleaning tank 911 through the third liquid inlet 9114, and the supply time of the second cleaning liquid is recorded. When the supply time of the second cleaning liquid reaches the second preset time, the waste liquid treatment component 94 is controlled to perform a second waste liquid treatment operation on the solution in the first cleaning tank 911 through the first drain outlet 9113. The second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first drain outlet 9113.

[0146] Optionally, before controlling the waste liquid treatment component 94 to perform the first waste liquid treatment operation, the control device 70 is further configured to: control the first liquid supply component 92 to stop supplying the first cleaning liquid to the first cleaning tank 911 when the liquid supply time of the first cleaning liquid reaches the first preset time; and before controlling the waste liquid treatment component 94 to perform the second waste liquid treatment operation, the control device 70 is further configured to: control the second liquid supply component 93 to stop supplying the second cleaning liquid to the first cleaning tank 911 when the liquid supply time of the second cleaning liquid reaches the second preset time.

[0147] like Figure 10As shown, in this embodiment, the pipette is first cleaned in the first cleaning tank 911 with a first cleaning solution. After cleaning the pipette with the first cleaning solution, the waste liquid in the first cleaning tank 911 is discharged through the waste liquid treatment unit 94. After the discharge is completed, the pipette is cleaned in the first cleaning tank 911 with a second cleaning solution. After cleaning the pipette with the second cleaning solution, the waste liquid in the first cleaning tank 911 is discharged through the waste liquid treatment unit 94 again.

[0148] Optionally, after the first cleaning tank 911 has finished draining, the waste liquid treatment component 94 is controlled to continue to be turned on for a preset time, so that the negative pressure formed by the waste liquid treatment component 94 at the first drain port 9113 can be used to dry the pipette located in the first cleaning tank 911.

[0149] After the waste liquid treatment component 94 has been open for a preset period of time, the control needle moving mechanism drives the pipette to move to the second cleaning tank 912, and controls the second liquid supply component 93 to supply the second cleaning liquid to the second cleaning tank 912, so as to clean the pipette located in the second cleaning tank 912 with the second cleaning liquid.

[0150] Please see Figure 11 In some embodiments, the pipette contains a target liquid, which is a salt solution. The first cleaning tank 911 is provided with a first inlet 9112 and a first outlet 9113. The cleaning device 90 also includes a waste liquid treatment component 94, which is connected to the first outlet 9113. A first supply component 92 is connected to the first inlet 9112. The cleaning device 90 is controlled to perform a first cleaning operation on the pipette located in the first cleaning tank 911, including: controlling the power mechanism to drive the pipette to discharge at least part of the target liquid into the first cleaning tank 911, and controlling the first supply component 92 to discharge at least part of the target liquid into the first cleaning tank 911. The liquid component 92 supplies the first cleaning liquid to the first cleaning tank 911 through the first liquid inlet 9112, so that the first cleaning liquid and the target liquid are mixed in the first cleaning tank 911 to form a mixed cleaning liquid; the liquid supply time of the first cleaning liquid is recorded, and when the liquid supply time of the first cleaning liquid reaches a third preset time, the waste liquid treatment component 94 is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank 911 through the first liquid outlet 9113. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first liquid outlet 9113 into the first cleaning tank 911.

[0151] Optionally, the concentration of the target liquid is greater than the concentration of the first cleaning liquid, and within a third preset time period, the pipette discharges a first volume of the target liquid into the first cleaning tank 911, and the first liquid supply component 92 supplies a second volume of the first cleaning liquid into the first cleaning tank 911, the second volume being greater than the first volume.

[0152] Optionally, after the pipette discharges at least part of the target liquid into the first cleaning tank 911, the control power mechanism drives the pipette to perform a back suction operation to back suction a preset volume of isolation air into the pipette, so as to prevent the target liquid from being thrown out of the pipette and contaminating the cleaning tank during the movement of the pipette, or to prevent the target liquid in the pipette from mixing with the cleaning liquid during the cleaning process in the cleaning tank, thereby reducing the liquid suction accuracy of the pipette.

[0153] For example, the target liquid is a lysis buffer, which can be used to clean proteinase K residues. During the process of the dispensing component 50 drawing up the lysis buffer, a preset amount of lysis buffer can be drawn up, such as drawing up at least a first volume of lysis buffer. During the process of the pipette cleaning the first cleaning pool 911, the first volume of lysis buffer is discharged into the first cleaning pool 911 through the pipette. In this way, not only can the inner wall of the pipette be cleaned by the discharged lysis buffer, but the outer wall of the pipette can also be cleaned by the mixture of the lysis buffer and the first cleaning buffer to form a mixed cleaning solution.

[0154] Optionally, the lysis buffer is a salt solution, and the concentration of the lysis buffer is 3-5 times the concentration of the first cleaning solution.

[0155] like Figure 12 As shown, optionally, the first cleaning tank 911 is provided with a third liquid inlet 9114, and the second liquid supply component 93 is connected to the third liquid inlet 9114; before the control waste liquid treatment component 94 performs the first waste liquid treatment operation, the control device 70 is further configured to: when the liquid supply time of the first cleaning liquid reaches a third preset time, control the second liquid supply component 93 to supply the second cleaning liquid to the first cleaning tank 911 through the third liquid inlet 9114, and record the liquid supply time of the second cleaning liquid; when the liquid supply time of the second cleaning liquid reaches a fourth preset time, control the waste liquid treatment component 94 to perform the first waste liquid treatment operation on the solution in the first cleaning tank 911 through the first liquid outlet 9113, wherein the first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first liquid outlet 9113 from the first cleaning tank 911.

[0156] In this embodiment, before the waste liquid treatment component 94 drains the liquid, the pipette is further cleaned with a second cleaning solution, thereby effectively reducing the adverse effects caused by the mixed cleaning solution that may remain on the surface of the pipette.

[0157] Optionally, before the waste liquid treatment component 94 performs the first waste liquid treatment operation, the control device 70 is further configured to: control the first liquid supply component 92 to stop supplying the first cleaning liquid to the first cleaning tank 911 and control the second liquid supply component 93 to supply the second cleaning liquid to the first cleaning tank 911 through the second liquid inlet when the liquid supply time of the first cleaning liquid reaches the third preset time; and control the second liquid supply component 93 to stop supplying the second cleaning liquid to the first cleaning tank 911 when the liquid supply time of the second cleaning liquid reaches the fourth preset time.

[0158] like Figure 13 As shown, optionally, the first cleaning tank 911 is provided with a third liquid inlet 9114, and the second liquid supply component 93 is connected to the third liquid inlet 9114; after the control waste liquid treatment component 94 performs the first waste liquid treatment operation, the control device 70 is further configured to: control the second liquid supply component 93 to supply the second cleaning liquid to the first cleaning tank 911 through the third liquid inlet 9114, and record the liquid supply duration of the second cleaning liquid; when the liquid supply duration of the second cleaning liquid reaches a fourth preset duration, control the waste liquid treatment component 94 to perform a second waste liquid treatment operation on the solution in the first cleaning tank 911 through the first liquid outlet 9113, wherein the second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first liquid outlet 9113 into the first cleaning tank 911.

[0159] In this embodiment, after the pipette is cleaned with the mixed cleaning solution, it is first drained through the waste liquid treatment component 94, and then cleaned with the second cleaning solution to effectively remove any residual mixed cleaning solution on the surface of the pipette. After the second cleaning solution is used to clean the pipette, it is drained through the waste liquid treatment component 94.

[0160] Optionally, before the waste liquid treatment component 94 performs the second waste liquid treatment operation, the control device 70 is further configured to: control the second liquid supply component 93 to stop supplying the second cleaning liquid to the first cleaning tank 911 when the liquid supply time of the second cleaning liquid reaches the fourth preset time.

[0161] Please see Figure 14 In some embodiments, the first cleaning tank 911 is provided with a first inlet 9112, a third inlet 9114, and a first outlet 9113. The cleaning device 90 also includes a waste liquid treatment component 94, which is connected to the first outlet 9113. A first liquid supply component 92 is connected to the first inlet 9112, and a second liquid supply component 93 is connected to the third inlet 9114. Controlling the cleaning device 90 to perform a first cleaning operation on the pipette located in the first cleaning tank 911 includes:

[0162] The system controls the first liquid supply component 92 to supply the first cleaning fluid to the first cleaning tank 911 through the first inlet 9112, and records the supply duration of the first cleaning fluid. When the supply duration of the first cleaning fluid reaches a first preset duration, the system controls the waste liquid treatment component 94 to perform a first waste liquid treatment operation on the solution in the first cleaning tank 911 through the first outlet 9113. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first outlet 9113. After the first waste liquid treatment operation is completed, the system controls the power mechanism to drive the pipette needle to discharge at least a portion of the target liquid into the first cleaning tank 911, and controls the first liquid supply component 92 to supply the first cleaning fluid to the first cleaning tank 911 through the first inlet 9112, so that the first cleaning fluid and the target liquid mix in the first cleaning tank 911 to form a mixed cleaning fluid. The supply duration of the first cleaning fluid is recorded. When the supply time of the first cleaning fluid reaches the third preset time, the waste liquid treatment component 94 is controlled to perform a second waste liquid treatment operation on the solution in the first cleaning tank 911 through the first drain port 9113. The second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first drain port 9113. After the second waste liquid treatment operation is completed, the second supply component 93 is controlled to supply the second cleaning fluid to the first cleaning tank 911 through the third inlet port 9114, and the supply time of the second cleaning fluid is recorded. When the supply time of the second cleaning fluid reaches the fourth preset time, the waste liquid treatment component 94 is controlled to perform a third waste liquid treatment operation on the solution in the first cleaning tank 911 through the first drain port 9113. The third waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank 911 through the first drain port 9113.

[0163] Optionally, the control device 70 is further configured to: control the first liquid supply component 92 to stop supplying the first cleaning liquid to the first cleaning tank 911 before performing the first waste liquid treatment operation;

[0164] And / or, before performing the second waste liquid treatment operation, control the first liquid supply component 92 to stop supplying the first cleaning liquid to the first cleaning tank 911;

[0165] And / or, before performing the third waste liquid treatment operation, control the second liquid supply component 93 to stop supplying the second cleaning liquid to the first cleaning tank 911.

[0166] Optionally, the concentration of the target liquid is greater than the concentration of the first cleaning liquid, and within a third preset time period, the pipette discharges a first volume of the target liquid into the first cleaning tank 911, and the first liquid supply component 92 supplies a second volume of the first cleaning liquid into the first cleaning tank 911, the second volume being greater than the first volume.

[0167] Optionally, the control device 70 is also used to control the power mechanism to drive the pipette to perform a back suction operation after the pipette discharges at least part of the target liquid into the first cleaning tank 911, so as to back suction a preset volume of isolation air into the pipette.

[0168] It is understood that the corresponding preset durations (e.g., the first preset duration, the second preset duration, the third preset duration, and the fourth preset duration) mentioned in the embodiments of this application can be set as needed, and are not limited here. For example, the preset duration can be any value between 0.2 and 3 seconds.

[0169] Please see Figure 15 In some embodiments, before the control device 90 performs the first cleaning operation on the pipette located in the cleaning tank, the control device 70 is further configured to:

[0170] The cleaning device 90 performs a third cleaning operation on the pipette located in the cleaning tank. The third cleaning operation includes at least controlling the second liquid supply component 93 to supply a second cleaning solution to the cleaning tank so as to clean the pipette at least by means of the second cleaning solution, and controlling the cleaning device 90 to perform an air drying operation on the pipette located in the cleaning tank after the third cleaning operation is completed.

[0171] For example, in this embodiment, in order to improve the cleaning effect of the pipette, after the dispensing component 50 completes the dispensing operation of the proteinase K reagent in the first target container, the control device 70 controls the cleaning device 90 to first pre-clean the pipette with the second cleaning solution, and after the pre-cleaning is completed, the cleaning tank is drained through the waste liquid treatment component 94, and a negative pressure is formed at the drain outlet, thereby driving the air flow at the drain outlet to dry the pipette. After the pipette is air-dried, it is cleaned with a first cleaning solution, and then further cleaned with a second cleaning solution. Since both the second and third cleaning solutions are salt solutions, and the concentration of the second cleaning solution is lower than that of the first, even if a high concentration of salt solution remains on the surface of the pipette after cleaning with the first solution, it can be diluted with the second cleaning solution. This not only improves the cleaning effect of the pipette but also effectively reduces the impact of the residual high concentration of salt solution on the activity of some enzyme reagents used in subsequent detection processes, ultimately improving the accuracy and reliability of sample detection results.

[0172] For example, the third and second cleaning operations can be performed in the second cleaning tank, while the first cleaning operation can be completed in the first cleaning tank. That is, after the dispensing component 50 completes the dispensing operation of the proteinase K reagent in the first target container, the control needle moving mechanism moves the pipette to the second cleaning tank 912, and controls the second liquid supply component 93 to supply the second cleaning solution to the second cleaning tank 912, so as to clean the pipette located in the second cleaning tank 912 at least by means of the second cleaning solution. After the pipette is cleaned, the waste liquid treatment component 94 drains the second cleaning tank and creates a negative pressure at the second drain outlet, thereby driving the airflow at the second drain outlet to dry the pipette in the second cleaning tank.

[0173] After the pipette is dried, the control needle moving mechanism moves the pipette to the first cleaning tank 911 and controls the cleaning device 90 to perform a first cleaning operation on the pipette located in the first cleaning tank 911. The first cleaning operation includes controlling the first liquid supply component 92 to supply a first cleaning solution to the first cleaning tank 911, so as to clean the pipette located in the first cleaning tank 911 at least through the first cleaning solution. After the first cleaning operation is completed, the control needle moving mechanism moves the pipette to the second cleaning tank 912 and controls the cleaning device 90 to perform a second cleaning operation on the pipette located in the second cleaning tank 912. The second cleaning operation includes controlling the second liquid supply component 93 to supply a second cleaning solution to the second cleaning tank 912, so as to clean the pipette located in the second cleaning tank 912 at least through the second cleaning solution.

[0174] It is understood that the first cleaning operation, the second cleaning operation, and the third cleaning operation can be completed in two different cleaning tanks or in the same cleaning tank; there are no restrictions here.

[0175] For example, taking the case where the first, second, and third cleaning operations are all performed in the first cleaning tank as an example, after the dispensing component 50 completes the dispensing operation of the proteinase K reagent in the first target container, the control needle moving mechanism moves the pipette to the first cleaning tank 911, and controls the second liquid supply component 93 to supply the second cleaning solution to the first cleaning tank 911, so as to clean the pipette located in the first cleaning tank 911 at least with the second cleaning solution. After the pipette is cleaned, the waste liquid treatment component 94 drains the first cleaning tank 911 and creates a negative pressure at the first drain port 9113, thereby driving the airflow at the first drain port 9113 to dry the pipette in the first cleaning tank 911.

[0176] After the pipette is dried, the first liquid supply component 92 is controlled to supply the first cleaning solution to the first cleaning tank 911, so as to clean the pipette located in the first cleaning tank 911 at least through the first cleaning solution; after the first cleaning operation is completed, the waste liquid treatment component 94 drains the first cleaning tank 911, and after the drainage is completed or during the drainage process, the second liquid supply component 93 is controlled to supply the second cleaning solution to the first cleaning tank 911, so as to clean the pipette located in the first cleaning tank 911 at least through the second cleaning solution.

[0177] Please see Figure 16 In some embodiments, the control device 70 is also used to: after the second cleaning operation is completed, control the cleaning device 90 to perform an air-drying operation on the pipette located in the cleaning tank.

[0178] Optionally, after the second cleaning operation is completed, the dispensing component 50 performs a second dispensing operation on the second target liquid in the second target container. The second target liquid includes at least DNA polymerase reagent and / or reverse transcriptase reagent. For example, if the first and second cleaning operations are performed in two different cleaning tanks, after the second cleaning operation is completed, the waste liquid treatment component 94 drains the second cleaning tank 912 and creates a negative pressure at the second drain port 9123, thereby causing airflow at the second drain port 9123 to dry the pipettes in the second cleaning tank 912.

[0179] After the pipette has dried, the needle moving mechanism moves the pipette to the location of the corresponding target container to aspirate the DNA polymerase reagent and / or reverse transcriptase reagent from the target container.

[0180] For example, the dispensing unit 50 performs a first dispensing operation on a first target liquid in a first target container, the first target liquid including at least proteinase K reagent; after the first dispensing operation is completed, the control needle moving mechanism moves the pipette to a first washing pool and controls the washing device 90 to perform a first washing operation on the pipette located in the washing pool, the first washing operation including controlling the first liquid supply unit 92 of the washing device 90 to provide a first washing solution to the first washing pool, so as to wash the pipette at least through the first washing solution; after the first washing operation is completed, the control needle moving mechanism moves the pipette to a second washing pool, and controls the washing device 90 to perform a second washing operation on the pipette located in the second washing pool, the second washing operation including controlling the second liquid supply unit 93 of the washing device 90 to provide a second washing solution to the second washing pool, so as to wash the pipette at least through the second washing solution; after the second washing operation is completed, the control dispensing unit 50 performs a second dispensing operation on a second target liquid in a second target container, the second target liquid including at least DNA polymerase reagent and / or reverse transcriptase reagent.

[0181] Please see Figure 17 In some embodiments, the control device 70 is further configured to: after the second cleaning operation is completed, control the dispensing component 50 to perform a second dispensing operation on the second target liquid in the second target container, wherein the second target liquid includes at least DNA polymerase reagent and / or reverse transcriptase reagent.

[0182] In this embodiment, with Figure 16 The corresponding implementation differs in that, after the second cleaning operation is completed, there is no need for needle drying. The needle moving mechanism drives the pipette to the location of the corresponding target container to aspirate the DNA polymerase reagent and / or reverse transcriptase reagent from the target container.

[0183] Please see Figure 18 In some embodiments, the first cleaning tank 911 includes a first inlet 9112 and a third inlet 9114. A first liquid supply component 92 is connected to the first inlet 9112 and is used to supply a first cleaning solution to the first cleaning tank 911 through the first inlet 9112. A second liquid supply component 93 is connected to the third inlet 9114 and is used to supply a second cleaning solution to the first cleaning tank 911 through the third inlet 9114. The cleaning device 90 is controlled to clean the pipette needle, including:

[0184] The control needle moving mechanism drives the pipette needle to the first cleaning tank 911, and controls the cleaning device 90 to perform a first cleaning operation on the pipette needle located in the first cleaning tank 911. The first cleaning operation includes at least controlling the first liquid supply component 92 to supply the first cleaning liquid to the first cleaning tank 911 through the first liquid inlet 9112, so as to clean the pipette needle at least by the first cleaning liquid.

[0185] After the first cleaning operation is completed, the first liquid supply component 92 is controlled to stop supplying liquid to the first cleaning tank 911, and the cleaning device 90 is controlled to perform a second cleaning operation on the pipette located in the first cleaning tank 911. The second cleaning operation includes at least controlling the second liquid supply component 93 to supply a second cleaning solution to the first cleaning tank 911 through the third liquid inlet 9114, so as to clean the pipette at least with the second cleaning solution.

[0186] This application also provides a method for cleaning a pipette, which can be applied to the aforementioned sample analyzer 100 and executed by the processor 701 in the control device 70 of the sample analyzer 100. For example, the corresponding program or instruction data is stored in the memory 702, and the processor 701 executes the method steps corresponding to the pipette cleaning method provided in this application embodiment by calling the program stored in the memory 702.

[0187] It should be noted that, for the sake of convenience and brevity, the steps for cleaning the pipette described below can be found in the working process of the control device 70 described above, and will not be repeated here.

[0188] Please see Figure 19 , Figure 19 This is a schematic flowchart of a pipette cleaning method provided in an embodiment of this application.

[0189] like Figure 19 As shown, the cleaning method for pipettes includes steps S101 to S102.

[0190] Step S101: Control the dispensing component of the sample analyzer to perform a dispensing operation on the target liquid in the target container;

[0191] Step S102: After the dispensing operation is completed, control the cleaning device of the sample analyzer to clean the pipette;

[0192] The step of controlling the cleaning device to clean the pipette includes:

[0193] The needle moving mechanism of the dispensing component is controlled to move the pipette needle to the cleaning tank of the cleaning device, and the cleaning device is controlled to perform a first cleaning operation on the pipette needle located in the cleaning tank. The first cleaning operation includes at least controlling the first liquid supply component of the cleaning device to supply a first cleaning solution to the cleaning tank, so as to clean the pipette needle at least by the first cleaning solution.

[0194] After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component of the cleaning device to supply a second cleaning solution to the cleaning tank, so as to clean the pipette at least by the second cleaning solution. Both the first cleaning solution and the second cleaning solution are salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution.

[0195] In some embodiments, the cleaning tank includes a first cleaning tank and a second cleaning tank, a first liquid supply component is used to supply a first cleaning solution to the first cleaning tank, a second liquid supply component is used to supply a second cleaning solution to the second cleaning tank, and the cleaning device is controlled to clean the pipette needle, including:

[0196] The control needle moving mechanism drives the pipette to move to the first cleaning pool and controls the cleaning device to perform a first cleaning operation on the pipette located in the first cleaning pool. The first cleaning operation includes at least controlling the first liquid supply component to supply the first cleaning liquid to the first cleaning pool, so as to clean the pipette located in the first cleaning pool at least by the first cleaning liquid.

[0197] After the first cleaning operation is completed, the control needle moving mechanism moves the pipette to the second cleaning tank and controls the cleaning device to perform a second cleaning operation on the pipette located in the second cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component to supply a second cleaning solution to the second cleaning tank, so as to clean the pipette located in the second cleaning tank at least by the second cleaning solution.

[0198] In some embodiments, the first cleaning tank is provided with a first inlet and a first outlet, and the cleaning device further includes a waste liquid treatment component connected to the first outlet, and a first supply component connected to the first inlet; controlling the cleaning device to perform a first cleaning operation on the pipette located in the first cleaning tank includes:

[0199] The first liquid supply component is controlled to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, and the liquid supply time of the first cleaning liquid is recorded.

[0200] When the supply time of the first cleaning fluid reaches the first preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port.

[0201] In some embodiments, before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the method further includes: controlling the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank.

[0202] In some embodiments, the first cleaning tank is provided with a first inlet, a third inlet, and a first outlet. The cleaning device further includes a waste liquid treatment component connected to the first outlet, a first liquid supply component connected to the first inlet, and a second liquid supply component connected to the third inlet. Controlling the cleaning device to perform a first cleaning operation on the pipette located in the first cleaning tank includes:

[0203] The first liquid supply component is controlled to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, and the liquid supply time of the first cleaning liquid is recorded.

[0204] When the supply time of the first cleaning fluid reaches the first preset time, the second liquid supply component is controlled to supply the second cleaning fluid to the first cleaning tank through the third liquid inlet, and the supply time of the second cleaning fluid is recorded.

[0205] When the supply time of the second cleaning fluid reaches the second preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port.

[0206] In some embodiments, before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the method further includes: when the supply time of the first cleaning liquid reaches a first preset time, controlling the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank, and controlling the second liquid supply component to supply the second cleaning liquid to the first cleaning tank through the second liquid inlet; and when the supply time of the second cleaning liquid reaches a second preset time, controlling the second liquid supply component to stop supplying the second cleaning liquid to the first cleaning tank.

[0207] In some embodiments, the first cleaning tank is provided with a first inlet, a second inlet, and a first outlet. The cleaning device further includes a waste liquid treatment component connected to the first outlet, a first liquid supply component connected to the first inlet, and a second liquid supply component connected to the second inlet. Controlling the cleaning device to perform a first cleaning operation on the pipette located in the first cleaning tank includes:

[0208] The first liquid supply component is controlled to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, and the liquid supply time of the first cleaning liquid is recorded.

[0209] When the supply time of the first cleaning fluid reaches the first preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

[0210] After the first waste liquid treatment operation is completed, the second liquid supply component is controlled to supply the second cleaning liquid to the first cleaning tank through the third liquid inlet, and the liquid supply time of the second cleaning liquid is recorded.

[0211] When the supply time of the second cleaning fluid reaches the second preset time, the waste liquid treatment component is controlled to perform a second waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port.

[0212] In some embodiments, before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the method further includes: when the supply time of the first cleaning liquid reaches a first preset time, controlling the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank;

[0213] Furthermore, before controlling the waste liquid treatment component to perform the second waste liquid treatment operation, the method further includes: when the supply time of the second cleaning liquid reaches a second preset time, controlling the second liquid supply component to stop supplying the second cleaning liquid to the first cleaning tank.

[0214] In some embodiments, the pipette contains a target liquid, which is a salt solution. A first cleaning tank is provided with a first inlet and a first outlet. The cleaning device further includes a waste liquid treatment component connected to the first outlet, and a first supply component connected to the first inlet. Controlling the cleaning device to perform a first cleaning operation on the pipette located in the first cleaning tank includes:

[0215] The control power mechanism drives the pipette to discharge at least part of the target liquid into the first cleaning tank, and controls the first liquid supply component to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, so that the first cleaning liquid and the target liquid are mixed in the first cleaning tank to form a mixed cleaning liquid;

[0216] Record the duration of the first cleaning fluid supply, and when the duration of the first cleaning fluid supply reaches the third preset duration, control the waste liquid treatment component to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

[0217] In some embodiments, the concentration of the target liquid is greater than the concentration of the first cleaning liquid, and within a third preset time period, the pipette discharges a first volume of the target liquid into the first cleaning tank, and the first liquid supply component supplies a second volume of the first cleaning liquid into the first cleaning tank, the second volume being greater than the first volume.

[0218] In some embodiments, the method further includes, after the pipette discharges at least part of the target liquid into the first cleaning tank, controlling the power mechanism to drive the pipette to perform a back suction operation to back suction a preset volume of isolation air into the pipette.

[0219] In some embodiments, the first cleaning tank is provided with a third liquid inlet, and the second liquid supply component is connected to the third liquid inlet; before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the method further includes:

[0220] When the supply time of the first cleaning fluid reaches the third preset time, the second liquid supply component is controlled to supply the second cleaning fluid to the first cleaning tank through the third liquid inlet, and the supply time of the second cleaning fluid is recorded.

[0221] When the supply time of the second cleaning fluid reaches the fourth preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port.

[0222] In some embodiments, the method further includes, before controlling the wastewater treatment component to perform the first wastewater treatment operation:

[0223] When the supply time of the first cleaning fluid reaches the third preset time, the first liquid supply component is controlled to stop supplying the first cleaning fluid to the first cleaning tank, and the second liquid supply component is controlled to supply the second cleaning fluid to the first cleaning tank through the third liquid inlet.

[0224] When the supply time of the second cleaning fluid reaches the fourth preset time, the second liquid supply component is controlled to stop supplying the second cleaning fluid to the first cleaning tank.

[0225] In some embodiments, the first cleaning tank is provided with a third liquid inlet, and the second liquid supply component is connected to the third liquid inlet; after the waste liquid treatment component performs the first waste liquid treatment operation, the method further includes:

[0226] The second liquid supply component is controlled to supply the second cleaning fluid to the first cleaning tank through the third liquid inlet, and the supply time of the second cleaning fluid is recorded.

[0227] When the supply time of the second cleaning fluid reaches the fourth preset time, the waste liquid treatment component is controlled to perform a second waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port.

[0228] In some embodiments, the method further includes, before controlling the waste liquid treatment component to perform the second waste liquid treatment operation:

[0229] When the supply time of the second cleaning fluid reaches the fourth preset time, the second liquid supply component is controlled to stop supplying the second cleaning fluid to the first cleaning tank.

[0230] In some embodiments, the pipette contains a target liquid, which is a salt solution. The first cleaning tank is provided with a first inlet, a third inlet, and a first outlet. The cleaning device also includes a waste liquid treatment component connected to the first outlet, a first liquid supply component connected to the first inlet, and a second liquid supply component connected to the third inlet. Controlling the cleaning device to perform a first cleaning operation on the pipette located in the first cleaning tank includes:

[0231] The first liquid supply component is controlled to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, and the liquid supply time of the first cleaning liquid is recorded.

[0232] When the supply time of the first cleaning fluid reaches the first preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

[0233] After the first waste liquid treatment operation is completed, the control power mechanism drives the pipetting needle to discharge at least part of the target liquid into the first cleaning tank, and controls the first liquid supply component to supply the first cleaning liquid into the first cleaning tank through the first liquid inlet, so that the first cleaning liquid and the target liquid are mixed in the first cleaning tank to form a mixed cleaning liquid;

[0234] Record the supply time of the first cleaning fluid, and when the supply time of the first cleaning fluid reaches the third preset time, control the waste liquid treatment component to perform a second waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

[0235] After the second waste liquid treatment operation is completed, the second liquid supply component is controlled to supply the second cleaning liquid to the first cleaning tank through the third liquid inlet, and the liquid supply time of the second cleaning liquid is recorded.

[0236] When the supply time of the second cleaning fluid reaches the fourth preset time, the waste liquid treatment component is controlled to perform a third waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The third waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port.

[0237] In some embodiments, the method further includes: controlling the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank before performing the first waste liquid treatment operation;

[0238] And / or, before performing the second waste liquid treatment operation, control the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank;

[0239] And / or, before performing the third waste liquid treatment operation, control the second liquid supply component to stop supplying the second cleaning liquid to the first cleaning tank.

[0240] In some embodiments, the concentration of the target liquid is greater than the concentration of the first cleaning liquid, and within a third preset time period, the pipette discharges a first volume of the target liquid into the first cleaning tank, and the first liquid supply component supplies a second volume of the first cleaning liquid into the first cleaning tank, the second volume being greater than the first volume.

[0241] In some embodiments, the method further includes, after the pipette discharges at least part of the target liquid into the first cleaning tank, controlling the power mechanism to drive the pipette to perform a back suction operation to back suction a preset volume of isolation air into the pipette.

[0242] In some embodiments, the method further includes, prior to controlling the cleaning device to perform a first cleaning operation on the pipette located in the cleaning tank:

[0243] The cleaning device performs a third cleaning operation on the pipette located in the cleaning tank. The third cleaning operation includes at least controlling the second liquid supply component to supply a second cleaning solution to the cleaning tank to clean the pipette at least by means of the second cleaning solution, and controlling the cleaning device to perform an air drying operation on the pipette located in the cleaning tank after the third cleaning operation is completed.

[0244] In some embodiments, the method further includes: after the second cleaning operation is completed, controlling the cleaning device to perform an air-drying operation on the pipette located in the cleaning tank.

[0245] In some embodiments, the target liquid includes at least a proteinase K reagent, or a solution containing a proteinase K reagent.

[0246] In some embodiments, the cleaning tank includes a first inlet and a third inlet, a first supply component is connected to the first inlet and is used to supply a first cleaning solution to the cleaning tank through the first inlet, and a second supply component is connected to the third inlet and is used to supply a second cleaning solution to the cleaning tank through the third inlet; controlling the cleaning device to clean the pipette includes:

[0247] The control needle moving mechanism drives the pipette needle to the cleaning tank and controls the cleaning device to perform a first cleaning operation on the pipette needle located in the cleaning tank. The first cleaning operation includes at least controlling the first liquid supply component to supply the first cleaning liquid to the cleaning tank through the first liquid inlet, so as to clean the pipette needle at least with the first cleaning liquid.

[0248] After the first cleaning operation is completed, the first liquid supply component is controlled to stop supplying liquid to the cleaning tank, and the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component to supply a second cleaning solution to the cleaning tank through a third liquid inlet, so as to clean the pipette at least with the second cleaning solution.

[0249] Please see Figure 20 , Figure 20 This is a schematic flowchart of a pipette cleaning method provided in an embodiment of this application.

[0250] like Figure 20 As shown, the cleaning method for pipettes includes steps S201 to S204.

[0251] Step S201: Control the dispensing component of the sample analyzer to perform a first dispensing operation on the first target liquid in the first target container, wherein the first target liquid includes at least proteinase K reagent;

[0252] Step S202: After the first dispensing operation is completed, the needle moving mechanism of the dispensing component is controlled to move the pipette to the cleaning pool, and the cleaning device of the sample analyzer is controlled to perform a first cleaning operation on the pipette located in the cleaning pool. The first cleaning operation includes controlling the first liquid supply component of the cleaning device to provide a first cleaning solution to the cleaning pool, so as to clean the pipette at least by the first cleaning solution.

[0253] Step S203: After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes controlling the second liquid supply component of the cleaning device to provide a second cleaning solution to the cleaning tank so as to clean the pipette at least by the second cleaning solution. The first cleaning solution and the second cleaning solution are both salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution.

[0254] Step S204: After the second cleaning operation is completed, the dispensing component is controlled to perform a second dispensing operation on the second target liquid in the second target container. The second target liquid includes at least DNA polymerase reagent and / or reverse transcriptase reagent.

[0255] It should be understood that the terminology used in this application specification is for the purpose of describing particular embodiments only and is not intended to limit the application. As used in this application specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0256] It should also be understood that the term "and / or" as used in this specification and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that, herein, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or system that includes that element.

[0257] The sequence numbers of the embodiments in this application are for descriptive purposes only and do not represent the superiority or inferiority of the embodiments. The above descriptions are merely specific implementations of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be covered within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A sample analyzer, characterized in that, include: A dispensing component is used to perform a dispensing operation, wherein the dispensing operation is used to aspirate a target liquid from a target container and discharge it into a corresponding reaction container; wherein the dispensing component includes a pipette, a needle moving mechanism, and a power mechanism, the needle moving mechanism is used to drive the pipette to move; the power mechanism is used to provide power for the pipette to aspirate and discharge the target liquid, wherein the target liquid includes at least one of a sample and a reagent; A measuring component is used to measure the reaction solution to obtain corresponding detection data, wherein the reaction solution is formed by mixing at least a sample and a reagent; The cleaning device includes a cleaning tank and a first liquid supply component and a second liquid supply component. The first liquid supply component is used to supply a first cleaning liquid to the cleaning tank, and the second liquid supply component is used to supply a second cleaning liquid to the cleaning tank. Control device, at least for: The dispensing component is controlled to perform the dispensing operation on the target liquid in the target container, and after the dispensing operation is completed, the cleaning device is controlled to clean the pipette needle; The step of controlling the cleaning device to clean the pipette includes: The needle moving mechanism is controlled to move the pipette to the cleaning tank, and the cleaning device is controlled to perform a first cleaning operation on the pipette located in the cleaning tank. The first cleaning operation includes at least controlling the first liquid supply component to supply the first cleaning solution to the cleaning tank, so as to clean the pipette at least by the first cleaning solution. After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component to supply the second cleaning solution to the cleaning tank so as to clean the pipette at least by the second cleaning solution. Both the first cleaning solution and the second cleaning solution are salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution.

2. The sample analyzer according to claim 1, characterized in that, The cleaning tank includes a first cleaning tank and a second cleaning tank. The first liquid supply component is used to supply the first cleaning solution to the first cleaning tank, and the second liquid supply component is used to supply the second cleaning solution to the second cleaning tank. Controlling the cleaning device to clean the pipette includes: The needle moving mechanism is controlled to move the pipette to the first cleaning pool, and the cleaning device is controlled to perform the first cleaning operation on the pipette located in the first cleaning pool. The first cleaning operation includes at least controlling the first liquid supply component to supply the first cleaning liquid to the first cleaning pool, so as to clean the pipette located in the first cleaning pool at least by the first cleaning liquid. After the first cleaning operation is completed, the needle moving mechanism is controlled to move the pipette to the second cleaning pool, and the cleaning device is controlled to perform the second cleaning operation on the pipette located in the second cleaning pool. The second cleaning operation includes at least controlling the second liquid supply component to supply the second cleaning liquid to the second cleaning pool, so as to clean the pipette located in the second cleaning pool at least by the second cleaning liquid.

3. The sample analyzer according to claim 2, characterized in that, The first cleaning tank is provided with a first inlet and a first outlet. The cleaning device further includes a waste liquid treatment component connected to the first outlet, and a first supply component connected to the first inlet. Controlling the cleaning device to perform the first cleaning operation on the pipette located in the first cleaning tank includes: Control the first liquid supply component to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, and record the liquid supply duration of the first cleaning liquid; When the supply time of the first cleaning fluid reaches the first preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

4. The sample analyzer according to claim 3, characterized in that, Before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the control device is further configured to: control the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank.

5. The sample analyzer according to claim 2, characterized in that, The first cleaning tank is provided with a first liquid inlet, a third liquid inlet, and a first liquid outlet. The cleaning device further includes a waste liquid treatment component connected to the first liquid outlet, a first liquid supply component connected to the first liquid inlet, and a second liquid supply component connected to the third liquid inlet. Controlling the cleaning device to perform the first cleaning operation on the pipette located in the first cleaning tank includes: Control the first liquid supply component to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, and record the liquid supply duration of the first cleaning liquid; When the supply time of the first cleaning fluid reaches the first preset time, the second liquid supply component is controlled to supply the second cleaning fluid to the first cleaning tank through the third liquid inlet, and the supply time of the second cleaning fluid is recorded. When the supply time of the second cleaning fluid reaches the second preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

6. The sample analyzer according to claim 5, characterized in that, Before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the control device is further configured to: When the supply time of the first cleaning fluid reaches the first preset time, the first supply component is controlled to stop supplying the first cleaning fluid to the first cleaning tank, and the second supply component is controlled to supply the second cleaning fluid to the first cleaning tank through the second inlet; and when the supply time of the second cleaning fluid reaches the second preset time, the second supply component is controlled to stop supplying the second cleaning fluid to the first cleaning tank.

7. The sample analyzer according to claim 2, characterized in that, The first cleaning tank is provided with a first liquid inlet, a second liquid inlet, and a first liquid outlet. The cleaning device further includes a waste liquid treatment component connected to the first liquid outlet, a first liquid supply component connected to the first liquid inlet, and a second liquid supply component connected to the second liquid inlet. Controlling the cleaning device to perform the first cleaning operation on the pipette located in the first cleaning tank includes: Control the first liquid supply component to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, and record the liquid supply duration of the first cleaning liquid; When the supply time of the first cleaning liquid reaches the first preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank. After the first waste liquid treatment operation is completed, the second liquid supply component is controlled to supply the second cleaning liquid to the first cleaning tank through the third liquid inlet, and the liquid supply time of the second cleaning liquid is recorded. When the supply time of the second cleaning fluid reaches the second preset time, the waste liquid treatment component is controlled to perform a second waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

8. The sample analyzer according to claim 7, characterized in that, Before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the control device is further configured to: control the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank when the liquid supply time of the first cleaning liquid reaches the first preset time. Furthermore, before controlling the waste liquid treatment component to perform the second waste liquid treatment operation, the control device is also configured to: control the second liquid supply component to stop supplying the second cleaning liquid to the first cleaning tank when the liquid supply time of the second cleaning liquid reaches the second preset time.

9. The sample analyzer according to claim 2, characterized in that, The pipette contains a target liquid, which is a salt solution. The first cleaning tank is provided with a first inlet and a first outlet. The cleaning device further includes a waste liquid treatment component connected to the first outlet, and a first liquid supply component connected to the first inlet. Controlling the cleaning device to perform the first cleaning operation on the pipette located in the first cleaning tank includes: The power mechanism is controlled to drive the pipette to discharge at least part of the target liquid into the first cleaning tank, and the first liquid supply component is controlled to supply the first cleaning liquid into the first cleaning tank through the first liquid inlet, so that the first cleaning liquid and the target liquid are mixed in the first cleaning tank to form a mixed cleaning liquid; Record the supply time of the first cleaning fluid, and when the supply time of the first cleaning fluid reaches a third preset time, control the waste liquid treatment component to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

10. The sample analyzer according to claim 9, characterized in that, The concentration of the target liquid is greater than the concentration of the first cleaning solution, and within the third preset time period, the pipette discharges a first volume of the target liquid into the first cleaning tank, and the first liquid supply component supplies a second volume of the first cleaning solution into the first cleaning tank, the second volume being greater than the first volume.

11. The sample analyzer according to claim 9, characterized in that, The control device is further configured to, after the pipette discharges at least part of the target liquid into the first cleaning tank, control the power mechanism to drive the pipette to perform a back suction operation, so as to back suction a preset volume of isolation air into the pipette.

12. The sample analyzer according to claim 9, characterized in that, The first cleaning tank is provided with a third liquid inlet, and the second liquid supply component is connected to the third liquid inlet; before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the control device is further used for: When the supply time of the first cleaning fluid reaches the third preset time, the second liquid supply component is controlled to supply the second cleaning fluid to the first cleaning tank through the third liquid inlet, and the supply time of the second cleaning fluid is recorded. When the supply time of the second cleaning fluid reaches the fourth preset time, the waste liquid treatment component is controlled to perform the first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

13. The sample analyzer according to claim 12, characterized in that, Before controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the control device is further configured to: When the supply time of the first cleaning fluid reaches the third preset time, the first liquid supply component is controlled to stop supplying the first cleaning fluid to the first cleaning tank, and the second liquid supply component is controlled to supply the second cleaning fluid to the first cleaning tank through the third liquid inlet. When the supply time of the second cleaning fluid reaches the fourth preset time, the second fluid supply component is controlled to stop supplying the second cleaning fluid to the first cleaning tank.

14. The sample analyzer according to claim 9, characterized in that, The first cleaning tank is provided with a third liquid inlet, and the second liquid supply component is connected to the third liquid inlet; after controlling the waste liquid treatment component to perform the first waste liquid treatment operation, the control device is further used to: The second liquid supply component is controlled to supply the second cleaning liquid to the first cleaning tank through the third liquid inlet, and the liquid supply duration of the second cleaning liquid is recorded; When the supply time of the second cleaning fluid reaches the fourth preset time, the waste liquid treatment component is controlled to perform a second waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

15. The sample analyzer according to claim 14, characterized in that, Before controlling the waste liquid treatment component to perform the second waste liquid treatment operation, the control device is further configured to: When the supply time of the second cleaning fluid reaches the fourth preset time, the second fluid supply component is controlled to stop supplying the second cleaning fluid to the first cleaning tank.

16. The sample analyzer according to claim 2, characterized in that, The pipette contains a target liquid, which is a salt solution. The first cleaning tank is provided with a first inlet, a third inlet, and a first outlet. The cleaning device further includes a waste liquid treatment component connected to the first outlet, a first liquid supply component connected to the first inlet, and a second liquid supply component connected to the third inlet. Controlling the cleaning device to perform the first cleaning operation on the pipette located in the first cleaning tank includes: Control the first liquid supply component to supply the first cleaning liquid to the first cleaning tank through the first liquid inlet, and record the liquid supply duration of the first cleaning liquid; When the supply time of the first cleaning liquid reaches the first preset time, the waste liquid treatment component is controlled to perform a first waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The first waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank. After the first waste liquid treatment operation is completed, the power mechanism is controlled to drive the pipette to discharge at least part of the target liquid into the first cleaning tank, and the first liquid supply component is controlled to supply the first cleaning liquid into the first cleaning tank through the first liquid inlet, so that the first cleaning liquid and the target liquid are mixed in the first cleaning tank to form a mixed cleaning liquid. Record the supply time of the first cleaning fluid, and when the supply time of the first cleaning fluid reaches a third preset time, control the waste liquid treatment component to perform a second waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The second waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank. After the second waste liquid treatment operation is completed, the second liquid supply component is controlled to supply the second cleaning liquid to the first cleaning tank through the third liquid inlet, and the liquid supply time of the second cleaning liquid is recorded. When the supply time of the second cleaning fluid reaches the fourth preset time, the waste liquid treatment component is controlled to perform a third waste liquid treatment operation on the solution in the first cleaning tank through the first drain port. The third waste liquid treatment operation discharges at least a portion of the solution in the first cleaning tank through the first drain port into the first cleaning tank.

17. The sample analyzer according to claim 16, characterized in that, The control device is further configured to: control the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank before performing the first waste liquid treatment operation; And / or, before performing the second waste liquid treatment operation, control the first liquid supply component to stop supplying the first cleaning liquid to the first cleaning tank; And / or, before performing the third waste liquid treatment operation, control the second liquid supply component to stop supplying the second cleaning liquid to the first cleaning tank.

18. The sample analyzer according to claim 17, characterized in that, The concentration of the target liquid is greater than the concentration of the first cleaning solution, and within the third preset time period, the pipette discharges a first volume of the target liquid into the first cleaning tank, and the first liquid supply component supplies a second volume of the first cleaning solution into the first cleaning tank, the second volume being greater than the first volume.

19. The sample analyzer according to claim 17, characterized in that, The control device is further configured to, after the pipette discharges at least part of the target liquid into the first cleaning tank, control the power mechanism to drive the pipette to perform a back suction operation, so as to back suction a preset volume of isolation air into the pipette.

20. The sample analyzer according to claim 2, characterized in that, The cleaning device also forms an overflow pool with a third opening and a third drain outlet. The third opening is located at the top of the overflow pool, and the third drain outlet is located on the side wall or bottom of the overflow pool. Liquid overflowing from the first cleaning pool and / or the second cleaning pool can enter the overflow pool through the third opening and be discharged from the overflow pool through the third drain outlet.

21. The sample analyzer according to claim 1, characterized in that, Before controlling the cleaning device to perform a first cleaning operation on the pipette located in the cleaning tank, the control device is further configured to: The cleaning device performs a third cleaning operation on the pipette located in the cleaning tank. The third cleaning operation includes at least controlling the second liquid supply component to supply the second cleaning solution to the cleaning tank to clean the pipette at least by means of the second cleaning solution, and controlling the cleaning device to perform an air-drying operation on the pipette located in the cleaning tank after the third cleaning operation is completed.

22. The sample analyzer according to claim 1, characterized in that, The control device is also used for: After the second cleaning operation is completed, the cleaning device is controlled to perform an air-drying operation on the pipette located in the cleaning tank.

23. The sample analyzer according to claim 1, characterized in that, The target liquid includes at least a proteinase K reagent, or a solution containing a proteinase K reagent.

24. The sample analyzer according to claim 1, characterized in that, The cleaning tank includes a first liquid inlet and a third liquid inlet. The first liquid supply component is connected to the first liquid inlet and is used to supply the first cleaning liquid to the cleaning tank through the first liquid inlet. The second liquid supply component is connected to the third liquid inlet and is used to supply the second cleaning liquid to the cleaning tank through the third liquid inlet. The control of the cleaning device to clean the pipette includes: The needle moving mechanism is controlled to move the pipette to the cleaning tank, and the cleaning device is controlled to perform the first cleaning operation on the pipette located in the cleaning tank. The first cleaning operation includes at least controlling the first liquid supply component to supply the first cleaning solution to the cleaning tank through the first liquid inlet, so as to clean the pipette at least through the first cleaning solution. After the first cleaning operation is completed, the first liquid supply component is controlled to stop supplying liquid to the cleaning tank, and the cleaning device is controlled to perform the second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component to supply the second cleaning liquid to the cleaning tank through the third liquid inlet, so as to clean the pipette at least with the second cleaning liquid.

25. A sample analyzer, characterized in that, include: A dispensing component is used to perform a dispensing operation, wherein the dispensing operation is used to aspirate a target liquid from a target container and discharge it into a corresponding reaction container; wherein the dispensing component includes a pipette, a needle moving mechanism, and a power mechanism, the needle moving mechanism is used to drive the pipette to move; the power mechanism is used to provide power for the pipette to aspirate and discharge the target liquid, wherein the target liquid includes at least one of a sample and a reagent; A measuring component is used to measure the reaction solution to obtain corresponding detection data, wherein the reaction solution is formed by mixing at least a sample and a reagent; The cleaning device includes a cleaning tank and a first liquid supply component and a second liquid supply component. The first liquid supply component is used to supply a first cleaning liquid to the cleaning tank, and the second liquid supply component is used to supply a second cleaning liquid to the cleaning tank. Control device, at least for: The dispensing component is controlled to perform a first dispensing operation on a first target liquid in a first target container, wherein the first target liquid includes at least proteinase K reagent; After the first dispensing operation is completed, the needle moving mechanism is controlled to move the pipette to the cleaning pool, and the cleaning device is controlled to perform a first cleaning operation on the pipette located in the cleaning pool. The first cleaning operation includes controlling the first liquid supply component of the cleaning device to provide a first cleaning solution to the cleaning pool, so as to clean the pipette at least by the first cleaning solution. After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes controlling the second liquid supply component of the cleaning device to provide a second cleaning solution to the cleaning tank, so as to clean the pipette at least by the second cleaning solution. The first cleaning solution and the second cleaning solution are both salt solutions, and the concentration of the second cleaning solution is less than that of the pipette. After the second cleaning operation is completed, the dispensing component is controlled to perform a second dispensing operation on the second target liquid in the second target container. The second target liquid includes at least DNA polymerase reagent and / or reverse transcriptase reagent.

26. A method for cleaning a pipette, applied to a sample analyzer, characterized in that, The method includes: The sample analyzer is controlled to perform a dispensing operation on the target liquid in the target container, and after the dispensing operation is completed, the sample analyzer is controlled to clean the pipette. The step of controlling the cleaning device to clean the pipette includes: The needle moving mechanism of the dispensing component is controlled to move the pipette needle to the cleaning tank of the cleaning device, and the cleaning device is controlled to perform a first cleaning operation on the pipette needle located in the cleaning tank. The first cleaning operation includes at least controlling the first liquid supply component of the cleaning device to supply a first cleaning solution to the cleaning tank, so as to clean the pipette needle at least by the first cleaning solution. After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes at least controlling the second liquid supply component of the cleaning device to supply a second cleaning solution to the cleaning tank, so as to clean the pipette at least by the second cleaning solution. Both the first cleaning solution and the second cleaning solution are salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution.

27. A method for cleaning a pipette, applied to a sample analyzer, characterized in that, The method includes: The sample analyzer is controlled to perform a first dispensing operation on a first target liquid in a first target container, wherein the first target liquid includes at least proteinase K reagent; After the first dispensing operation is completed, the needle moving mechanism of the dispensing component is controlled to move the pipette to the cleaning pool, and the cleaning device of the sample analyzer is controlled to perform a first cleaning operation on the pipette located in the cleaning pool. The first cleaning operation includes controlling the first liquid supply component of the cleaning device to provide a first cleaning solution to the cleaning pool, so as to clean the pipette at least by the first cleaning solution. After the first cleaning operation is completed, the cleaning device is controlled to perform a second cleaning operation on the pipette located in the cleaning tank. The second cleaning operation includes controlling the second liquid supply component of the cleaning device to provide a second cleaning solution to the cleaning tank, so as to clean the pipette at least by the second cleaning solution. The first cleaning solution and the second cleaning solution are both salt solutions, and the concentration of the second cleaning solution is less than the concentration of the first cleaning solution. After the second cleaning operation is completed, the dispensing component is controlled to perform a second dispensing operation on the second target liquid in the second target container. The second target liquid includes at least DNA polymerase reagent and / or reverse transcriptase reagent.

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