An organic selenium fertilizer based on L-selenium-methylselenocysteine and application thereof in selenium-rich crop planting

Abstract

This invention provides an organic selenium fertilizer based on L-seleno-methylselenocysteine ​​and its application in the cultivation of selenium-enriched crops, relating to the field of organic selenium fertilizer technology. The organic selenium fertilizer based on L-seleno-methylselenocysteine ​​comprises, by weight percentage: 1.1%~3.0% L-seleno-methylselenocysteine, 75%~88.4% compound amino acids, 10%~20% amino acid chelated trace elements, and 0.5%~2.0% special adjuvants; the purity of the L-seleno-methylselenocysteine ​​is ≥98%, the organic selenium content in the composition is ≥98% of the total selenium, and the special adjuvants include anti-caking agents and stabilizers. This invention, by selecting high-purity L-seleno-methylselenocysteine ​​as the core selenium source and synergistically combining it with compound amino acids, amino acid chelated trace elements, and special adjuvants, achieves the technical effects of high-efficiency selenium enrichment at low concentrations, high conversion of organic selenium, and targeted application to crops, demonstrating good prospects for industrialization and promotion.

Description

Technical Field

[0001] This invention relates to the field of organic selenium fertilizer technology, and in particular to an organic selenium fertilizer based on L-seleno-methylselenocysteine ​​and its application in the cultivation of selenium-rich crops. Background Technology

[0002] Selenium-enriched agricultural products are experiencing a continuously expanding market demand due to their significant health benefits, such as supplementing the human body with the essential trace element selenium, enhancing immune function, and boosting antioxidant capacity. Currently, low-concentration selenium fertilizers used in agricultural production generally suffer from the following problems: the selenium source mainly relies on inorganic selenium or low-purity organic selenium, with the proportion of organic selenium typically being low, resulting in insufficient safety and bioavailability; under low-concentration application conditions, crop selenium enrichment efficiency is low; the formulation lacks targeted design for the selenium absorption characteristics of different crops, leading to unstable and inconsistent selenium enrichment effects; some products employ microbial fermentation processes, resulting in complex compositions and low purity controllability, making it difficult to meet the high purity and high safety requirements of high-end selenium-enriched agricultural products.

[0003] L-Seleno-methylselenocysteine, as a high-quality organic selenium source, boasts advantages such as low toxicity, high bioavailability, and easy absorption and efficient conversion by plants. However, existing low-concentration selenium fertilizers have not yet used it as a core selenium source, and the purity of organic selenium is difficult to consistently achieve high levels, failing to fully meet the production needs of high-end selenium-enriched agricultural products. Furthermore, existing selenium enrichment technologies for specific crops (such as sesame) mostly employ the combination of inorganic selenium and yeast selenium or natural raw material extraction methods, which suffer from complex operating processes, low organic selenium purity, and insufficient targeting. Summary of the Invention

[0004] In view of this, the present invention provides an organic selenium fertilizer based on L-seleno-methylselenocysteine, its preparation method, and its application. By selecting high-purity L-seleno-methylselenocysteine ​​as the core selenium source and synergistically combining it with compound amino acids, amino acid chelated trace elements, and special adjuvants, the present invention achieves the technical effects of high-efficiency selenium enrichment at low concentrations, high-proportion conversion of organic selenium, and targeted application to crops, demonstrating good prospects for industrialization and promotion.

[0005] The first aspect of this invention provides an organic selenium fertilizer based on L-seleno-methylselenocysteine, comprising the following components by weight percentage: L-Selenium-methylselenocysteine ​​1.1%~3.0%, complex amino acids 75%~88.4%, amino acid chelated trace elements 10%~20%, and special additives 0.5%~2.0%; The purity of the L-seleno-methylselenocysteine ​​is ≥98%, and the proportion of organic selenium in the composition is ≥98% of the total selenium.

[0006] Preferably, the composite amino acid includes at least one of glycine, leucine, methionine, tyrosine, histidine, threonine, alanine, isoleucine, tryptophan, cysteine, lysine, aspartic acid, valine, phenylalanine, proline, serine, glutamic acid, arginine, glutamine, asparagine, and hydrolyzed plant protein amino acids; the free amino acid content of the composite amino acid is ≥90%, and the proportion of glutamic acid is ≥30%.

[0007] Preferably, the amino acid chelated trace element is at least one selected from amino acid chelated iron, amino acid chelated zinc, amino acid chelated copper, and amino acid chelated manganese; more preferably, the amino acid is glycine.

[0008] Preferably, the special additive includes an anti-caking agent and a stabilizer; more preferably, the anti-caking agent is fumed silica and the stabilizer is vitamin C; even more preferably, the mass ratio of fumed silica to vitamin C is 1:1~2.

[0009] A second aspect of this invention provides a method for preparing the above-mentioned organic selenium fertilizer based on L-seleno-methylselenocysteine, comprising the following steps: S1. Take the formula amount of compound amino acids and amino acid chelated trace elements and add them to a rotating double cone oven. Cover the oven and stir at 60°C for 2-3 hours. Then cool to room temperature. S2. Add the prescribed amount of L-seleno-methylselenocysteine ​​and stir continuously for 1-2 hours to form a homogeneous mixture; then pulverize the mixture and mix it again with a rotating double cone for 2-3 hours. S3. Add the special additives according to the formula, continue to rotate and stir for 2-3 hours, filter through a 100-mesh filter, and fill to obtain the finished product.

[0010] The third aspect of this invention provides the application of the above-mentioned organic selenium fertilizer based on L-seleno-methylselenocysteine ​​in the cultivation of selenium-rich crops.

[0011] Preferably, the crop is rice, tea, vegetables or sesame, and the L-seleno-methylselenocysteine ​​organic selenium fertilizer is applied by foliar spraying.

[0012] Preferably, for rice, spray once each during the booting and grain-filling stages, diluted 100-300 times, with a dosage of 50-100 g per mu; for tea, spray once each during the spring shoot emergence stage, the one-bud-one-leaf stage, and 10-15 days before harvest, diluted 100-300 times, with a dosage of 100-200 g per mu; for vegetables, spray once each during the seedling stage, the growth stage, and 7-10 days before harvest, diluted 300-1000 times, with a dosage of 50-100 g per mu; and for sesame, spray once each during the initial flowering stage, the full bloom stage, and the maturity stage, diluted 100-300 times, with a dosage of 50-100 g per mu.

[0013] Preferably, the rice is indica rice, japonica rice, or hybrid rice; the tea is green tea, white tea, black tea, or oolong tea; the vegetables are leafy vegetables, fruit vegetables, or root vegetables; and the sesame is white sesame or black sesame.

[0014] Preferably, the leafy vegetables are spinach or lettuce; the fruit vegetables are tomatoes or cucumbers; the root vegetables are radishes or carrots; the white sesame seeds are Zhongzhi 13 or Zhongzhi 16; and the black sesame seeds are Zhongzhi 9 or Ganzhi 3.

[0015] Compared with the prior art, the beneficial technical effects of the present invention are as follows: This invention effectively solves the prominent problems of insufficient organic selenium purity, low selenium enrichment efficiency, and poor crop targeting in traditional low-concentration selenium fertilizers by selecting high-purity L-seleno-methylselenocysteine ​​as the core selenium source and synergistically compounding it with a specific ratio of compound amino acids, amino acid chelated trace elements, and special adjuvants, thus achieving a "low concentration, high efficiency" selenium enrichment effect.

[0016] This invention uses a powdered formula with good water solubility, making it easy to apply as a foliar spray. It is suitable for the selenium enrichment production of various crops, and can significantly increase the organic selenium content and proportion of edible parts of crops. The product is highly safe and leaves no soil residue, meeting the requirements of green agricultural development.

[0017] The formula of this invention has good stability, meets the NY 1429 water-soluble fertilizer standard, is easy to store and transport, is simple to operate, and is suitable for large-scale promotion and application. It provides reliable technical support for the large-scale production of high-end selenium-rich agricultural products and has good industrial application value. Detailed Implementation

[0018] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0019] Unless otherwise stated, all experiments were repeated three times, and the results are expressed as averages.

[0020] Example 1: Organic selenium fertilizer suitable for rice The organic selenium fertilizer in this embodiment comprises, by weight percentage: L-Selenium-methylselenocysteine ​​1.2%, complex amino acids 87.8%, amino acid chelated trace elements 10%, and special additives 1.0%; The compound amino acids include glycine, glutamic acid, aspartic acid, proline, and lysine; the amino acid chelated trace element is zinc glycinate; and the special additives are fumed silica and vitamin C, with a mass ratio of 1:1.

[0021] The preparation method is as follows: 1) Take 87.8 kg of the compound amino acids and 10 kg of amino acid chelated trace elements according to the formula and add them to a rotating double cone oven. Cover the oven and stir at 60°C for 2.5 h. Then cool to room temperature.

[0022] 2) Open the lid, add 1.2 kg of L-seleno-methylselenocysteine, and stir continuously for 1.5 h to form a homogeneous mixture; then pulverize it and mix it again with a rotating double cone for 2.5 h.

[0023] 3) Finally, add 1 kg of special additive, continue to rotate and stir for 2.5 h, filter through a 100-mesh filter, and fill to obtain the finished product.

[0024] Application effect: In indica rice cultivation, spray once each during the booting and grain-filling stages, diluted 200 times, with a dosage of 50g per mu (approximately 0.067 hectares). Post-harvest testing showed that the organic selenium content of the rice was 0.22 mg / kg, with an organic selenium content of 99.7%.

[0025] Example 2: Organic selenium fertilizer suitable for tea The difference from Example 1 is that: Organic selenium fertilizer, by weight percentage, includes: The formula consists of 2% L-seleno-methylselenocysteine, 82% complex amino acids, 15% amino acid chelated trace elements, and 1.0% special additives. The complex amino acids include glycine, glutamic acid, aspartic acid, and glutamine; the amino acid chelated trace element is zinc glycinate; and the special additives are fumed silica and vitamin C, in a 1:1 mass ratio.

[0026] Application effects: In green tea cultivation, spray once each during the spring shoot emergence stage, the one-bud-one-leaf stage, and 10 days before harvesting, diluted 300 times, with a dosage of 100 g per acre. Post-harvest testing showed that the organic selenium content of the dry tea leaves was 2.0 mg / kg, accounting for 99.2% of the total organic selenium content. The tea polyphenol content increased by 11.5%, and the sensory quality score improved by 7.8 points (scoring criteria: appearance 2 points, liquor color 2 points, aroma 1 point, taste 1.8 points, and infused leaf 1 point).

[0027] Example 3: Organic selenium fertilizer suitable for leafy vegetables The difference from Example 1 is that: Organic selenium fertilizer is suitable for leafy vegetables such as lettuce. By weight percentage, it comprises: 1.5% L-seleno-methylselenocysteine, 85% complex amino acids, 12% amino acid chelated trace elements, and 1.5% special additives. The complex amino acids are hydrolyzed plant protein amino acids (Maclean, catalog number: A741933, acid-hydrolyzed plant protein, amino acid nitrogen ≥2.3 g / 100g) and glutamic acid, with a mass ratio of 1:1; the amino acid chelated trace elements are glycine-chelated iron, glycine-chelated zinc, and glycine-chelated copper, with a mass ratio of 1:1:1; and the special additives are fumed silica and vitamin C, with a mass ratio of 1:2.

[0028] Application effects: In lettuce cultivation, spray once each during the seedling stage, growth stage, and 7 days before harvest, diluted 1000 times, with a dosage of 80g per acre. Post-harvest testing showed that the organic selenium content in the edible portion of lettuce was 0.15 mg / kg, accounting for 98.9% of the total organic selenium, and the vitamin C content increased by 14.3%.

[0029] Example 4: Organic Selenium Fertilizer Suitable for Sesame The difference from Example 1 is that the organic selenium fertilizer in this example is suitable for sesame; by weight percentage, it includes: 1.1% L-seleno-methylselenocysteine, 88% compound amino acids, 10% amino acid chelated trace elements, and 0.9% special adjuvants. Among them, the compound amino acids are 18 kinds of amino acids (35% essential amino acids and 65% non-essential amino acids, with an even distribution within the essential amino acid group and an even distribution within the non-essential amino acid group); the amino acid chelated trace elements are glycine chelated iron and glycine chelated zinc, with a mass ratio of 1:1; the special adjuvants are fumed silica and vitamin C, with a mass ratio of 1:1.

[0030] Application effects: In the cultivation of Ganzhi No. 3 black sesame, the product was sprayed once each at the initial flowering stage, full bloom stage, and maturity stage, diluted 100 times, with a dosage of 50 g per mu. After harvest, the organic selenium content of the sesame seeds was 0.50 mg / kg, the organic selenium ratio was 99.8%, and the oil content increased by 5.7%.

[0031] Comparative Example 1 The difference from Example 1 is that a commercially available low-concentration organic selenium fertilizer is used, which is a yeast selenium source, and the application dosage is consistent with that of the present invention.

[0032] Test results: The crop was rice, the selenium fertilizer contained 0.5% selenium, the organic selenium accounted for 89.5%, the crop selenium content was 0.16 mg / kg, and the yield increase rate was 2.3%.

[0033] Comparative Example 2 The difference from Example 1 is that the crop is sesame, and the existing sesame selenium enrichment technology is used. The existing sesame selenium enrichment technology is a combination of yeast selenium and sodium selenite, and the application dosage is consistent with that of the present invention.

[0034] Test results: The selenium fertilizer contained 0.6% selenium, with organic selenium accounting for 88.0%, and the selenium content in the crop was 0.28 mg / kg, with a yield increase rate of 3.1%.

[0035] Table 1 Comparison of selenium enrichment effects among different treatment groups

[0036] Note: "—" indicates that the yield increase rate was not measured.

[0037] Comparative Example 3 The difference from Example 1 is that L-seleno-methylselenocysteine ​​is replaced with an equal amount of selenomethionine (purity ≥98%), while the other components and application methods remain the same as in Example 1.

[0038] Test results: The organic selenium content of rice was 0.12 mg / kg, the organic selenium content was 99.1%, and the yield increase rate was 3.2%.

[0039] Comparative Example 4 The difference from Example 1 is that only L-seleno-methylselenocysteine ​​is dissolved in water and sprayed directly on the leaves, without adding compound amino acids, amino acid chelated trace elements and special adjuvants, and the amount of selenium applied is the same as in Example 1.

[0040] Test results: The organic selenium content of rice was 0.18 mg / kg, the organic selenium content was 99.5%, and the yield increase rate was 3.7%.

[0041] Comparative Example 5 The difference from Example 1 is that L-seleno-methylselenocysteine ​​is replaced with sodium selenite, and the types and ratios of the compound amino acids and amino acid chelated trace elements are in accordance with the foliar selenium fertilizer formula in Example 1 of CN201210333089.4. The total selenium application amount is the same as in Example 1, and other application methods are the same.

[0042] Test results: The organic selenium content of rice was 0.06 mg / kg, the proportion of organic selenium was 36%, and the yield increase rate was 2.5%.

[0043] Comparative Example 6 The difference from Example 1 is that L-seleno-methylselenocysteine ​​with a purity of ≥98% is replaced with L-seleno-methylselenocysteine ​​with a purity of 90% (the organic selenium content is comparable to that of existing low-purity organic selenium fertilizers), while the other components and application methods remain the same as in Example 1.

[0044] Test results: The organic selenium content of rice was 0.19 mg / kg, the proportion of organic selenium was 98.2%, and the yield increase rate was 4.6%.

[0045] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. An organoselenium fertilizer based on L-selenium-methylselenocysteine, characterized in that, By weight percentage, it includes the following components: L-Selenium-methylselenocysteine ​​1.1%~3.0%, complex amino acids 75%~88.4%, amino acid chelated trace elements 10%~20%, and special additives 0.5%~2.0%; The purity of the L-seleno-methylselenocysteine ​​is ≥98%, the proportion of organic selenium in the composition is ≥98% of the total selenium, and the special additives include anti-caking agents and stabilizers.

2. The organic selenium fertilizer based on L-selenium-methylselenocysteine according to claim 1, characterized in that, The complex amino acids include at least one of glycine, leucine, methionine, tyrosine, histidine, threonine, alanine, isoleucine, tryptophan, cysteine, lysine, aspartic acid, valine, phenylalanine, proline, serine, glutamic acid, arginine, glutamine, asparagine, and hydrolyzed plant protein amino acids.

3. The organic selenium fertilizer based on L-selenium-methylselenocysteine according to claim 1, characterized by that, The amino acid chelated trace element is at least one of amino acid chelated iron, amino acid chelated zinc, amino acid chelated copper, and amino acid chelated manganese.

4. The organic selenium fertilizer based on L-selenium-methylselenocysteine according to claim 1, characterized by that, The anti-caking agent is fumed silica, and the stabilizer is vitamin C.

5. The method for preparing organic selenium fertilizer based on L-seleno-methylselenocysteine ​​according to any one of claims 1 to 4, characterized in that, Includes the following steps: S1. Take the formula amount of compound amino acids and amino acid chelated trace elements and add them to a rotating double cone oven. Cover the oven and stir at 60°C for 2-3 hours. Then cool to room temperature. S2. Add the prescribed amount of L-seleno-methylselenocysteine ​​and stir continuously for 1-2 hours to form a homogeneous mixture; then pulverize the mixture and mix it again with a rotating double cone for 2-3 hours. S3. Add the special additives according to the formula, continue to rotate and stir for 2-3 hours, filter through a 100-mesh filter, and fill to obtain the finished product.

6. The application of the organic selenium fertilizer based on L-seleno-methylselenocysteine ​​as described in any one of claims 1 to 4 in the cultivation of selenium-enriched crops.

7. The application according to claim 6, characterized in that, The crops mentioned are rice, tea, vegetables, or sesame.

8. The application according to claim 7, characterized in that, The L-selenium-methylselenocysteine ​​organic selenium fertilizer is applied by foliar spraying.

9. The application according to claim 8, characterized in that, For rice, spray once each during the booting and grain-filling stages, diluted 100-300 times, with a dosage of 50-100 g per mu (approximately 0.067 hectares). For tea, spray once each during the spring shoot emergence stage, the one-bud-one-leaf stage, and 10-15 days before harvest, diluted 100-300 times, with a dosage of 100-200 g per mu (approximately 0.067 hectares). For vegetables, spray once each during the seedling stage, the growth stage, and 7-10 days before harvest, diluted 300-1000 times, with a dosage of 50-100 g per mu (approximately 0.067 hectares). For sesame, spray once each during the initial flowering stage, the full bloom stage, and the maturity stage, diluted 100-300 times, with a dosage of 50-100 g per mu (approximately 0.067 hectares).

10. The application according to claim 6, characterized in that, The rice is indica rice, japonica rice, or hybrid rice; the tea is green tea, white tea, black tea, or oolong tea; the vegetables are leafy vegetables, fruit vegetables, or root vegetables; and the sesame is white sesame or black sesame.

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