A ligand binding protein and its preparation method and application

By designing a ligand-binding protein that targets MMLV reverse transcriptase, the problem of gDNA noise in RNA sequencing library construction was solved, achieving efficient nucleic acid sorting and improving the purity of NGS library construction and the reliability of sequencing data.

CN122325565APending Publication Date: 2026-07-03ACCURATE BIOTECHNOLOGY(HUNAN) CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ACCURATE BIOTECHNOLOGY(HUNAN) CO LTD
Filing Date
2026-06-04
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

In the current RNA NGS sequencing library preparation process, residual genomic DNA (gDNA) in the sample acts as background noise, which seriously reduces the effective utilization rate of sequencing data of target nucleic acids, increases sequencing costs, and affects the accuracy of analysis.

Method used

A ligand-binding protein was designed to target the positively charged nucleic acid binding pocket of MMLV reverse transcriptase through electrostatic complementarity, significantly improving the enzyme's stability and enabling it to efficiently and specifically inhibit gDNA interference and sort nucleic acids under mild conditions.

Benefits of technology

It significantly improves the purity of NGS library preparation and the reliability of sequencing data, reduces gDNA background noise, and promotes the industrial application of NGS library preparation.

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Abstract

This invention discloses a ligand-binding protein, its preparation method, and its applications, relating to the field of biotechnology. Specifically, this invention discloses a ligand-binding protein of any one of a1) to a3): a1) a protein with the amino acid sequence shown in SEQ ID NO:1; a2) a protein with more than 90% identity and the same activity as the protein described in a1) obtained by substituting and / or deleting and / or adding amino acid residues to the sequence shown in SEQ ID NO:1; a3) a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of a1) or a2). It can efficiently and specifically suppress gDNA interference, reduce gDNA background noise during NGS library construction, and improve the purity of the library and the reliability of subsequent sequencing data.
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