A natural plant essential oil insecticide composition, its preparation method and application

Abstract

This invention relates to the field of grain storage technology, and in particular to a natural plant essential oil insecticide composition, its preparation method, and its application. The natural plant essential oil insecticide composition comprises a main active ingredient and co-active ingredients. The main active ingredient includes cinnamaldehyde, allyl isothiocyanate, and cuminaldehyde. The co-active ingredients include 1,8-cineole, β-phellandrene, γ-terpinene, p-cymene, eugenol, α-pinene, linalool, and limonene. By volume percentage, the insecticide composition contains: cinnamaldehyde 15-40%, allyl isothiocyanate 20-40%, and cuminaldehyde 0.5-16.5%. The insecticide composition provided by this invention is made from natural plant essential oils, is stable, safe, environmentally friendly, does not pollute grains, and has an aromatic odor. It has a strong insecticidal effect on pests such as rice weevils in grains and has good application prospects in the field of grain storage.

Description

Technical Field

[0001] This invention relates to the field of grain storage technology, and in particular to a natural plant essential oil insecticide composition, its preparation method, and its application. Background Technology

[0002] Numerous problems arise during long-term grain storage, with grain pests being a significant factor contributing to grain loss. The rice weevil (Sitophilus oryzae) is one of the most widespread and destructive grain pests. Grain infested with this pest during storage is easily hollowed out, leading to severe quantity and quality losses, rendering the grain inedible and commercially worthless, resulting in substantial economic losses. Currently, grain pest control relies heavily on phosphine fumigation, which, while fast-acting, carries risks such as pesticide resistance, excessive residues, and poisoning of workers. Summary of the Invention

[0003] The purpose of this invention is to provide a natural plant essential oil insecticide composition, its preparation method, and its application, in order to solve the problems existing in the prior art.

[0004] To achieve the above objectives, the present invention provides the following solution: One of the technical solutions of this invention is a natural plant essential oil insecticide composition, comprising a main active ingredient and a co-active ingredient; the main active ingredient comprises cinnamaldehyde, allyl isothiocyanate, and cuminaldehyde; the co-active ingredients comprise 1,8-cineole, β-phellandrene, γ-terpinene, p-cymene, eugenol, α-pinene, linalool, and limonene; by volume percentage, the insecticide composition comprises: cinnamaldehyde 15-40%, allyl isothiocyanate 20-40%, and cuminaldehyde 0.5-16.5%; the main active ingredient comprises not less than 55% by volume in the insecticide composition.

[0005] The natural plant essential oil insecticide composition provided by this invention uses cinnamaldehyde, allyl isothiocyanate, and cuminaldehyde as the main active ingredients. Through multi-target synergistic action, it significantly enhances the insecticidal effect. Cinnamaldehyde acts on the nervous system of pests, interfering with their normal conduction; allyl isothiocyanate has strong penetrating and fumigating effects, destroying the respiratory and nervous systems of pests and exerting a rapid killing effect; cuminaldehyde effectively interferes with the metabolic enzyme system and odor recognition of pests. The synergistic effect of these three ingredients achieves extremely high pest mortality rates at low application concentrations, while also effectively delaying the development of pesticide resistance. Regarding pest repellency, the strong pungent odor of allyl isothiocyanate effectively repels rice weevils; cinnamaldehyde blocks rice weevils' ability to recognize the odor of rice grains by interfering with olfactory receptor proteins; and cuminaldehyde reduces the rice weevils' willingness to bore into grains to lay eggs. The combination of these three ingredients forms a complex odor network in the grain storage environment, making it difficult for rice weevils to adapt, thereby significantly reducing their invasion and egg-laying rates. In pest control, the rapid fumigation killing power of mustard oil (allyl isothiocyanate), the sustained contact killing power of cinnamon oil (cinnamaldehyde), and the highly efficient penetration ability of cumin oil (cuminaldehyde, α-pinene, β-pinene, and other active substances) are combined to achieve significant insecticidal effects. Furthermore, regarding resistance, cinnamaldehyde interferes with pest development, allyl isothiocyanate interferes with the nervous system, and cuminaldehyde inhibits enzyme activity, making it difficult for pests to develop resistance through a single gene mutation. The natural plant essential oil insecticide composition provided by this invention also includes co-active ingredients, which assist the main active ingredient in controlling stored grain pests: 1,8-cineole, although not effective in contact insecticides, can enhance its insecticidal effect when combined with cinnamaldehyde, and can reduce the pungent odor of allyl isothiocyanate when combined with it. β-phellandrene can inhibit the reproduction of rice weevils to a certain extent and has a certain repellent effect on the coexistence of cymene and γ-terpinene in plant essential oils.

[0006] Preferably, by volume percentage, the insecticide composition comprises: 15-40% cinnamaldehyde, 20-40% allyl isothiocyanate, 0.5-16.5% cumin, 0.5-24.5% 1,8-cineole, 0.4-13% β-phellandrene, 0.4-13% γ-terpinene, 0.3-8.5% p-cymene, 0.2-5% eugenol, 0.1-4.5% α-pinene, 0.1-1.5% linalool, and 0.1-1.5% limonene.

[0007] When the insecticide composition obtained within the above-described scope of the present invention was used in a contact killing test on rice weevils, the results showed that the corrected mortality rate reached 100% after 12 hours at a concentration of 128 μL / mL.

[0008] Preferably, by volume percentage, the insecticide composition comprises: 15-40% cinnamaldehyde, 20-40% allyl isothiocyanate, 0.5-16.5% cumin, 0.5-5% 1,8-cineole, 0.4-13% β-phellandrene, 0.4-13% γ-terpinene, 0.3-8.5% p-cymene, 0.2-5% eugenol, 0.1-4.5% α-pinene, 0.1-1.5% linalool, and 0.1-1.5% limonene.

[0009] When the insecticide composition obtained within the above-described scope of the present invention was used in a contact killing test on rice weevils, the results showed that the corrected mortality rate reached over 93% after 12 hours at a concentration of 32 μL / mL.

[0010] Preferably, by volume percentage, the insecticide composition comprises: 29.9% cinnamaldehyde, 22.2% allyl isothiocyanate, 12.1% cuminaldehyde, 0.5% 1,8-cineole, 12.1% β-phellandrene, 12.1% γ-terpinene, 8.1% p-cymene, 2.5% eugenol, 0.1% α-pinene, 0.3% linalool, and 0.1% limonene.

[0011] When the insecticide composition obtained within the above-described scope of the present invention was used in a contact killing test on rice weevils, the results showed that the corrected mortality rate reached 93.4% after 12 hours at a concentration of 32 μL / mL.

[0012] The second technical solution of the present invention, the preparation method of the above-mentioned natural plant essential oil insecticide composition, includes the following steps: Cinnamaldehyde, allyl isothiocyanate, cuminaldehyde, 1,8-cineole, β-phellandrene, γ-terpinene, p-cymene, eugenol, α-pinene, linalool, and limonene were taken separately and mixed evenly to obtain the insecticide composition.

[0013] The third technical solution of the present invention is the application of the above-mentioned natural plant essential oil insecticide composition in the prevention and control of stored grain pests.

[0014] Preferably, the grain is treated by fumigation using the insecticide composition.

[0015] Preferably, the fumigation concentration is 8-12 μL / L, the solvent used for fumigation is anhydrous ethanol, and the fumigation time is 45-50 h.

[0016] Preferably, the pest is the rice weevil.

[0017] Preferably, the fumigation is carried out by atomization or natural evaporation.

[0018] The present invention discloses the following technical effects: The insecticide composition provided by this invention is made from natural plant essential oils. It is stable, safe, green and environmentally friendly, does not pollute grains, and has an aromatic odor. It has a strong insecticidal and repellent effect on pests such as rice weevils in grains and has good application prospects in the field of grain storage.

[0019] The insecticide composition provided by this invention uses natural plant essential oils as raw materials. Plant essential oils are secondary metabolites with special aromatic odors extracted from plants. These secondary metabolites are formed by the plant's own defense capabilities and the adaptive evolution of insects. Therefore, pests are less likely to develop resistance. In addition, plant essential oils are volatile, easily degradable, and have the characteristics of low toxicity and no pollution, which are in line with the concept of green grain storage and have high application value. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 The corrected mortality rate of the insecticide composition prepared in Example 2 of this invention at different concentrations; Figure 2 The corrected mortality rate of the insecticide composition prepared in Example 3 of this invention at different concentrations. Detailed Implementation

[0022] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0023] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included within the scope of this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0024] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0025] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be readily apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0026] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0027] Example 1 A natural plant essential oil insecticide composition is prepared by measuring the raw materials in the following volume proportions, stirring until fully mixed, to obtain the insecticide composition: 29.9 parts cinnamaldehyde, 22.2 parts allyl isothiocyanate, 12.1 parts cuminaldehyde, 0.5 parts 1,8-cineole, 12.1 parts β-phellandrene, 12.1 parts γ-terpinene, 8.1 parts p-cymene, 2.5 parts eugenol, 0.1 parts α-pinene, 0.3 parts linalool, and 0.1 parts limonene.

[0028] Example 2 A natural plant essential oil insecticide composition is prepared by measuring the raw materials in the following volume proportions, stirring until fully mixed, to obtain the insecticide composition: 35.5 parts cinnamaldehyde, 30 parts allyl isothiocyanate, 0.5 parts cuminaldehyde, 24.3 parts 1,8-cineole, 0.4 parts β-phellandrene, 0.4 parts γ-terpinene, 0.8 parts p-cymene, 2.4 parts eugenol, 4.1 parts α-pinene, 0.8 parts linalool, and 0.8 parts limonene.

[0029] Example 3 A natural plant essential oil insecticide composition is prepared by measuring the raw materials in the following volume proportions, stirring until fully mixed, to obtain the insecticide composition: 15 parts cinnamaldehyde, 34.5 parts allyl isothiocyanate, 8.2 parts cuminaldehyde, 21.4 parts 1,8-cineole, 6.5 parts β-phellandrene, 6.5 parts γ-terpinene, 4.2 parts p-cymene, 0.2 parts eugenol, 3 parts α-pinene, 0.1 parts linalool, and 0.4 parts limonene.

[0030] Comparative Example 1 An insecticide composition, the only raw material being cinnamaldehyde.

[0031] Comparative Example 2 An insecticide composition, the only raw material being allyl isothiocyanate.

[0032] Comparative Example 3 An insecticide composition, the only raw material of which is calcinaldehyde.

[0033] Comparative Example 4 An insecticide composition, the only raw material being cypermethrin.

[0034] Comparative Example 5 Same as Example 1, except that the total volume of the main active ingredient remains unchanged, and cinnamaldehyde and allyl isothiocyanate in a volume ratio of 1:1 are used as the main active ingredient (cinnamaldehyde is omitted): Cinnamaldehyde 32.1 parts, allyl isothiocyanate 32.1 parts, 1,8-cineole 0.5 parts, β-phellandrene 12.1 parts, γ-terpinene 12.1 parts, p-cymene 8.1 parts, eugenol 2.5 parts, α-pinene 0.1 parts, linalool 0.3 parts, limonene 0.1 parts.

[0035] Comparative Example 6 Same as Example 1, except that the total volume of the main active ingredient remains unchanged, and cinnamaldehyde and cuminaldehyde in a volume ratio of 1:1 are used as the main active ingredients (allyl isothiocyanate is omitted): Cinnamaldehyde 32.1 parts, Cuminaldehyde 32.1 parts, 1,8-Cineole 0.5 parts, β-Phaseol 12.1 parts, γ-Tene 12.1 parts, p-Cymenene 8.1 parts, Eugenol 2.5 parts, α-Pinene 0.1 parts, Linalool 0.3 parts, Limonene 0.1 parts.

[0036] Comparative Example 7 Same as Example 1, except that the total volume of the main active ingredient remains unchanged, and allyl isothiocyanate and cuminaldehyde are used as the main active ingredients in a volume ratio of 1:1 (cinnamaldehyde is omitted): Allyl isothiocyanate 32.1 parts, cuminaldehyde 32.1 parts, 1,8-cineole 0.5 parts, β-phellandrene 12.1 parts, γ-terpinene 12.1 parts, p-cymene 8.1 parts, eugenol 2.5 parts, α-pinene 0.1 parts, linalool 0.3 parts, limonene 0.1 parts.

[0037] Comparative Example 8 Same as Example 1, except that the total volume of the main active ingredient remains unchanged, and the volume ratio of cinnamaldehyde, allyl isothiocyanate, and cuminaldehyde is adjusted to 1:1:1, that is: Cinnamaldehyde 21.4 parts, allyl isothiocyanate 21.4 parts, cuminaldehyde 21.4 parts, 1,8-cineole 0.5 parts, β-phellandrene 12.1 parts, γ-terpinene 12.1 parts, p-cymene 8.1 parts, eugenol 2.5 parts, α-pinene 0.1 parts, linalool 0.3 parts, limonene 0.1 parts.

[0038] Experimental verification results I. The contact toxicity of the insecticide composition against rice weevils was verified. The specific experimental procedure is as follows: (1) Test insect: Rice weevil (adults 1-2 weeks after emergence); (2) Rearing of test insects: The test insects provided by Guangzhou Panyu Grain Reserve Co., Ltd. were reared in a constant temperature insect rearing room in the laboratory (temperature 28±1℃, RH 75%±5%). Under dark conditions, whole rice grains were used as feed, which were placed in wide-mouthed bottles, and adult insects were introduced into them and placed in the insect rearing room. After 7 days, the adult insects were screened out, and the rearing continued (for about 35 days). After a large number of new generation adult insects appeared, 5-10 days later, healthy adult insects were selected as test insects, and a portion of the test insects were screened out every 14 days.

[0039] (3) The insecticide composition to be tested was diluted with acetone (acetone was used because of its volatility; ethanol is equally effective) to 8, 16, 32, 64, and 128 μL / mL. A 9 cm culture dish was selected, and a piece of filter paper of equal area was placed on the bottom of the dish. 0.5 mL of the prepared insecticide composition solution was taken with a micropipette and spread evenly on the 9 cm diameter filter paper from the outside in. After 30 seconds (to allow the acetone to evaporate completely), it was glued to the bottom of the culture dish with a solid glue stick. Twenty test insects were evenly placed on the filter paper, the dish was covered, and the dish was placed in a constant temperature insect rearing room for culture. The acetone treatment was used as a control group. Each sample treatment group and control group were repeated 3 times, and the above contact killing method was used for treatment. The number of dead insects in each dish was observed and recorded after 12h, 24h, 36h, 48h, 60h, and 72h. The mortality standard was determined by lightly touching the antennae or legs of the test insect with a brush; if there was no reaction, it was considered dead. The mortality rate and the corrected mortality rate were calculated.

[0040] The formulas for mortality rate and adjusted mortality rate are as follows: In the above formula, the mortality rate of the control group refers to the mortality rate when only acetone is used for treatment.

[0041] II. The repellent effect of the insecticide composition on rice weevils was verified. The specific experimental procedure is as follows: The insecticide composition to be validated was diluted with acetone to a concentration of 0.50 ± 0.01 μL / cm² on filter paper. A 9 cm petri dish was used as the experimental container, cut into two semicircles along its diameter. One half of the dish was treated with 400 μL of the solution, while the other half was immersed in acetone as a negative control. After allowing the solution to evaporate for 30 seconds, the two semicircles were reassembled and placed over the petri dish. Ten insects were released into the center of the dish, which was then covered. To minimize the influence of potential insect attraction, the direction of the seam was randomly selected for each of the three replicate experiments. The number of insects on the filter paper was recorded at 15 min, 30 min, 1 h, and 2 h, and the repulsion rate was calculated using the following formula: III. To verify the control effect of the insecticide composition on grain pests (rice weevils) using fumigation, the specific experimental procedure is as follows: The insecticide composition to be validated was diluted with anhydrous ethanol to prepare a 25% (v / v) dilution. 20 μL of this dilution was dropped onto the middle of a 10 cm × 1 cm filter paper strip using a microsyringe. The filter paper strip was then attached to the mouth of a 500 mL bottle (the concentration of essential oil in the bottle was 10 μL / L). After standing for 30 seconds to evaporate, 20 insects and 12 g of rice were added to each bottle. The bottle cap was then tightened and sealed. The reagent bottle was then transferred to an incubator with the same culture conditions (temperature 28±1℃, RH 75%±5%) and fumigated for 48 h. The filter paper strip with 20 μL of anhydrous ethanol was used as a negative control. The number of dead insects and the total number of insects were recorded, and the mortality rate and corrected mortality rate were calculated.

[0042] The formulas for mortality rate and adjusted mortality rate are as follows: The experimental verification results are statistically summarized as follows: (1) Trigger effect A. The corrected mortality rates of the insecticidal composition of Example 1 at 8, 16, 32, 64, and 128 μL / mL were 0%, 11.94%, 93.40%, 100%, and 100% at 12 hours, and 1.59%, 25.44%, 96.40%, 100%, and 100% at 24 hours, respectively.

[0043] B. Corrected mortality rates of the insecticide composition prepared in Example 2 at different concentrations are shown in [reference needed]. Figure 1 , Figure 1The results showed that the formulated insecticide was not very effective in killing rice weevils at low concentrations, but at a concentration of 32 μL / mL, the corrected mortality rate exceeded 50% on day 2.5, and at 64 μL / mL after 1.5 days, the corrected mortality rate was close to 50%, and close to 100% on day 3. At a concentration of 128 μL / mL, the corrected mortality rate was close to 100% at 12 hours.

[0044] C. Corrected mortality rates of the insecticide composition prepared in Example 3 at different concentrations are shown in [reference needed]. Figure 2 , Figure 2 The results showed that at the 12th hour of the experiment, the corrected mortality rates of the insecticides at concentrations of 8, 16, 32, 64, and 128 μL / mL were 1.34%, 1.76%, 1.76%, 38.33%, and 100.00%, respectively. On the third day, the corrected mortality rates were 5.73%, 12.00%, 24.00%, 69.17%, and 100%, respectively. This indicates that as time increases, the components in the insecticide begin to take effect, achieving a relatively good contact killing effect.

[0045] Therefore, the natural plant essential oil-based grain pesticides provided by this invention can achieve a 100% corrected mortality rate at a concentration of 128 μL / mL. In the preferred embodiment 1, the corrected mortality rate reached 93.4% after 12 hours at a concentration of 32 μL / mL.

[0046] D. The contact toxicity of different insecticide compositions at a treatment concentration of 32 μL / mL for 12 h is shown in Table 1.

[0047] Analysis of the data in Table 1 shows that cinnamaldehyde and allyl isothiocyanate exhibited good contact toxicity against rice weevils at a concentration of 32 μL / mL after 12 hours, with corrected mortality rates of 90% and 86.67%, respectively. The insecticide formulated with these two components and calciferol also demonstrated good contact toxicity. Furthermore, the proportions of cinnamaldehyde, allyl isothiocyanate, and calciferol in the formulated insecticide were 29.9%, 22.2%, and 12.1%, respectively, indicating that the combined use of cinnamaldehyde, allyl isothiocyanate, and calciferol had a synergistic effect on rice weevils. Cypermethrin, at a concentration of 32 μL / mL, achieved 100% mortality at 12 hours, but its odor was very pungent. The aromatic scent of naturally extracted plant essential oil-based grain insecticides was more acceptable. In Comparative Examples 5-7, the absence of a certain main active ingredient prevented the synergistic effect from being achieved, resulting in a decrease in corrected mortality rate even when the amount of the main active ingredient remained unchanged. In Comparative Example 8, the synergistic effect could not be achieved due to the significant adjustment of the proportion of the main active ingredient, thus correcting for the decrease in mortality.

[0048] In the above statistics, the mortality rate of the control group treated with acetone was 1.25%.

[0049] (2) Approach-avoidance effect Analyzing the data in Table 2, the concentration ranged from 0.49 to 0.51 μL / cm. 2 At the specified concentrations, both Examples 1 and 2 exhibited sustained and stable repellent effects against rice weevils within 120 minutes. Example 1 showed a 100% repellency rate at 15 minutes, higher than any other combination, demonstrating significant synergistic effects, and maintained a 93.33% repellency rate at 120 minutes. Example 2 maintained a 93.33% repellency rate at 15-30 minutes and 120 minutes, achieving complete repellency at 60 minutes. Example 3 had the lowest repellency rate at 46.85% at 60 minutes, but the repellent component began to take effect with prolonged time. Comparing the repellency rates at 15 minutes, Examples 1 and 2 were higher than those of a single main active ingredient; Example 3 was higher than Comparative Examples 1 and 2, but lower than Comparative Example 3. However, since cucurbitacin in Example 3 only accounted for 8.2%, the repellent effect of Example 3 was higher than that of a single ingredient. Initially, each embodiment of the invention exerted an insect-repelling effect, causing rice weevils to leave and reducing their chances of contacting rice grains and laying eggs. As time went on, the insect-repelling effect weakened, and the contact-killing effect began to take effect.

[0050] (3) Fumigation effect Analysis of the data in Table 3 shows that cinnamaldehyde and allyl isothiocyanate exhibited good fumigation activity against rice weevils at a concentration of 10 μL / L after 48 hours, with corrected mortality rates of 89.76% and 100%, respectively. The insecticide formulated with these two components and cumin also demonstrated good fumigation efficacy. The corrected mortality rates for rice weevils achieved by fumigation in Examples 1, 2, and 3 were all 100%.

[0051] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A natural plant essential oil insecticide composition, characterized in that, Including the main active ingredient and the co-active ingredient; The main active ingredients include cinnamaldehyde, allyl isothiocyanate and cuminaldehyde. The co-active ingredients include 1,8-cineole, β-phellandrene, γ-terpinene, p-cymene, eugenol, α-pinene, linalool and limonene; By volume percentage, the insecticide composition contains: 15-40% cinnamaldehyde, 20-40% allyl isothiocyanate, and 0.5-16.5% cuminaldehyde; the main active ingredient in the insecticide composition is not less than 55% by volume.

2. The natural plant essential oil insecticide composition according to claim 1, characterized in that, The insecticide composition, by volume percentage, comprises: cinnamaldehyde 15-40%, allyl isothiocyanate 20-40%, cuminaldehyde 0.5-16.5%, 1,8-cineole 0.5-24.5%, β-phellandrene 0.4-13%, γ-terpinene 0.4-13%, p-cymene 0.3-8.5%, eugenol 0.2-5%, α-pinene 0.1-4.5%, linalool 0.1-1.5%, and limonene 0.1-1.5%.

3. The natural plant essential oil insecticide composition according to claim 2, characterized in that, The insecticide composition, by volume percentage, comprises: cinnamaldehyde 15-40%, allyl isothiocyanate 20-40%, cuminaldehyde 0.5-16.5%, 1,8-cineole 0.5-5%, β-phellandrene 0.4-13%, γ-terpinene 0.4-13%, p-cymene 0.3-8.5%, eugenol 0.2-5%, α-pinene 0.1-4.5%, linalool 0.1-1.5%, and limonene 0.1-1.5%.

4. The natural plant essential oil insecticide composition according to claim 3, characterized in that, The insecticide composition, by volume percentage, comprises: cinnamaldehyde 29.9%, allyl isothiocyanate 22.2%, cuminaldehyde 12.1%, 1,8-cineole 0.5%, β-phellandrene 12.1%, γ-terpinene 12.1%, p-cymene 8.1%, eugenol 2.5%, α-pinene 0.1%, linalool 0.3%, and limonene 0.1%.

5. A method for preparing a natural plant essential oil insecticide composition according to any one of claims 1-4, characterized in that, Includes the following steps: Cinnamaldehyde, allyl isothiocyanate, cuminaldehyde, 1,8-cineole, β-phellandrene, γ-terpinene, p-cymene, eugenol, α-pinene, linalool, and limonene were taken separately and mixed evenly to obtain the insecticide composition.

6. The application of a natural plant essential oil insecticide composition according to any one of claims 1-4 in the control of stored grain pests.

7. The application according to claim 6, characterized in that, The insecticide composition is used to treat grains by fumigation.

8. The application according to claim 7, characterized in that, The fumigation concentration is 8-12 μL / L, the solvent used for fumigation is anhydrous ethanol, and the fumigation time is 45-50 h.

9. The application according to claim 6, characterized in that, The pest in question is the rice weevil.

10. The application according to claim 7, characterized in that, The fumigation is carried out by atomization or natural evaporation.

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