A rich regulatory miRNA drug-loaded astragalus outer vesicle-like nanoparticle and a preparation method and application thereof
By preparing Astragalus mesenchymal stem cell vesicle-like nanoparticles rich in regulatory miRNA, the problems of low survival rate of mesenchymal stem cells and limited solubility of Astragalus mesenchymal components were solved, improving the therapeutic effect of knee osteoarthritis, enhancing the survival rate and migration ability of mesenchymal stem cells in hypoxic environments, and reducing the risk of tumorigenesis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANXI UNIV OF CHINESE MEDICINE
- Filing Date
- 2026-05-15
- Publication Date
- 2026-07-17
AI Technical Summary
In existing technologies, mesenchymal stem cells have a low survival rate in the treatment of knee osteoarthritis, and the limited solubility and low bioavailability of Astragalus membranaceus components result in limited therapeutic effects.
Astragalus vesicle-like nanoparticles rich in regulatory miRNA were prepared. Astragalus saponins were extracted and loaded using enzymatic hydrolysis, enzyme inactivation, filtration, and centrifugation to form nanoparticles with high protein content and a bilayer cup-bottom structure. These nanoparticles were used to enhance the viability and migration ability of mesenchymal stem cells under hypoxic conditions.
It improved the survival rate and therapeutic effect of mesenchymal stem cells in the treatment of knee osteoarthritis, inhibited the abnormal changes of cells caused by the inflammatory microenvironment, reduced the risk of tumorigenesis, and enhanced the therapeutic effect of osteoarthritis.
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Figure CN122398752A_ABST