Reagent compositions and methods of preparing lyophilized reagents
By combining TMPD, trehalose, mannitol, disodium EDTA, and glycine, and utilizing liquid nitrogen droplet and freeze-drying processes, the stability and shaping issues of TMPD solution were resolved, resulting in the preparation of a lyophilized reagent with intact morphology and stable activity.
CN122405786APending Publication Date: 2026-07-17WENZHOU MICRODOME MICROBIAL TECH CO LTD
View PDF 0 Cites 0 Cited by
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- WENZHOU MICRODOME MICROBIAL TECH CO LTD
- Filing Date
- 2026-06-12
- Publication Date
- 2026-07-17
Smart Images
Figure CN122405786A_ABST
Abstract
本发明公开了一种试剂组合物及冻干试剂的制备方法，该试剂组合物包含TMPD、海藻糖、甘露醇、EDTA二钠、甘氨酸和注射用水，该冻干试剂的制备方法包括：提供该试剂组合物；在避光条件下，将该试剂组合物滴入液氮中，使其结晶形成圆球状的冰珠；将该圆球状的冰珠置于预冷的冻干机层板上进行冷冻干燥，得到冻干物；向冻干机内充入氮气，取出冻干物并密封保存。本发明的有益效果为：通过各组分的合理配伍，使试剂组合物能够适应液氮滴珠和冷冻干燥工艺，所得冻干物形态完整、活性稳定，缓解了单纯TMPD溶液冻干时活性易受损、制剂无法成型的问题。
Need to check novelty before this filing date? Find Prior Art