A method for quantitative analysis of living cell FRET two-hybrid
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SOUTH CHINA NORMAL UNIV
- Filing Date
- 2026-01-21
- Publication Date
- 2026-07-17
AI Technical Summary
Existing FRET two-hybrid quantitative analysis methods are difficult to accurately characterize binding heterogeneity in complex biological systems, leading to underfitting and parameter inversion bias. This is especially true in low-concentration regions where the signal-to-noise ratio is high and the error proportion is large. The nonlinear fitting process is unstable, making it difficult to achieve accurate characterization of the binding mechanism.
The Langmuir-Freundlich model is introduced and combined with weighted least squares and regularization penalty terms. The heterogeneity index n is used to characterize the difference in energy distribution of binding sites. The non-uniform binding quantitative relationship between the corrected FRET energy transfer efficiency and the concentration of free molecules is established. A robust inversion strategy is adopted to suppress the non-uniform distribution of errors and parameter coupling.
It improves the quantitative modeling capability for heterogeneous binding systems, realizes the quantitative characterization of binding heterogeneity, outputs quantifiable heterogeneity indices, solves the ill-conditioned problem of the fitting process, and improves the reliability of parameter inversion and the repeatability of results.
Smart Images
Figure CN122405795A_ABST