Chemiluminescent substrate solution for detecting alkaline phosphatase and preparation method and use thereof

By introducing BHT/BHA and alkyltributyl quaternary phosphine salt into the alkaline phosphatase chemiluminescent substrate solution, the problems of high background noise and poor storage stability were solved, and high-sensitivity detection with low background noise and long-term stability was achieved.

CN122409628APending Publication Date: 2026-07-17JINHUA XINKE PHARMA TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JINHUA XINKE PHARMA TECH CO LTD
Filing Date
2026-04-24
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing alkaline phosphatase chemiluminescent substrate solutions suffer from high background noise and poor storage stability, affecting detection sensitivity and accuracy.

Method used

A chemiluminescent substrate solution was prepared by using butylated hydroxytoluene (BHT) and/or butylated hydroxyanisole (BHA) as background inhibitors, combined with alkyl tributyl quaternary phosphine salt as luminescence enhancer, and adjusting the pH value to 8–10.5.

Benefits of technology

It significantly reduces background noise, improves the signal-to-noise ratio, extends storage stability, and enhances detection sensitivity and the reliability of detection results.

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Abstract

本申请公开了一种用于检测碱性磷酸酶的化学发光底物液及其制备方法和用途。所述化学发光底物液，包括以下组分：0.1～0.3 g / L的化学发光底物、0.05～0.15 g / L的荧光素类化合物、0.1～1.0 g / L的发光增强剂、0.01～0.1 g / L的背景抑制剂和0.3～1.5 g / L的添加剂，余量为缓冲剂和水，所述pH调节剂使得所述化学发光底物液的pH值为8～10.5；所述背景抑制剂为二丁基羟基甲苯和 / 或丁基羟基茴香醚。本申请的化学发光底物液显著降低体系的本底噪声及储存期间的本底升高速率，在维持高发光信号强度的同时，大幅提升了检测的信噪比、灵敏度及储存稳定性，成功解决了现有技术中高本底与稳定性差的技术难题，特别适用于高灵敏度免疫分析和分子杂交检测。
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