A segmentation method for segmenting high-density microreactor images

By combining frequency domain preprocessing and U-Net network with geometric vector field branching, the problems of size perturbation and adhesion in high-density microreactor image segmentation are solved, achieving high-precision and robust image segmentation results.

CN122415633APending Publication Date: 2026-07-17SHANGHAI INSTITUTE OF INFECTIOUS DISEASE & BIOSECURITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI INSTITUTE OF INFECTIOUS DISEASE & BIOSECURITY
Filing Date
2026-04-10
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing image processing algorithms struggle to achieve accurate segmentation in high-density microreactor scenarios, especially under complex fluorescence backgrounds and noise interference. Traditional methods suffer from quantitative errors and lack adaptability to different imaging environments.

Method used

Frequency domain preprocessing combined with U-Net network is used to suppress background noise and enhance the contour features of microreactors through high-pass filter. Geometric vector field branch is introduced for foreground localization and geometric topology-guided segmentation. Cosine similarity loss and average diameter constraint are used to achieve accurate segmentation.

Benefits of technology

It effectively resists size disturbances and adhesion crosstalk, enabling precise segmentation of high-density microreactor images, improving segmentation accuracy and robustness, and adapting to microreactors of different shapes and sizes.

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Abstract

本发明提供一种用于分割高密度微反应器图像的分割方法,包括:将原始图像处理得到频域增强特征图;将原始图像与频域增强特征图的局部图拼接后输入第一U‑Net网络,包括分割概率分支和几何向量场分支,以输出第一二值前景掩码和第一二维预测向量场;后处理得到初始平均直径,以其作为先验约束,拼接结果输入第二U‑Net网络,以输出第二二值前景掩码和第二几何向量场;后处理得到最终中心坐标和最终平均直径,得到分割结果;几何向量场分支仅在前景区域计算余弦相似度损失来得到几何向量场损失,使得第二U‑Net网络中的预测向量模长由初始平均直径来约束。本发明的方法能够有效抗尺寸扰动,抗粘连串扰,实现精准分割。
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