A method for sweat glucose concentration monitoring

By combining an enzyme-immobilized strand conjugate with a circular DNA template and amplifying a self-assembled DNA hydrogel using rolling circle amplification, the problem of immobilizing the enzyme active center was solved, and a flexible bioelectrode was constructed, enabling efficient and self-powered monitoring of sweat glucose concentration, suitable for home health monitoring.

CN122428018APending Publication Date: 2026-07-21DEZHOU UNIV
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Patent Information

Application Number
CN202610619134.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-07
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

Existing sweat glucose sensing technologies suffer from enzyme immobilization strategies that lead to conformational changes and activity loss at the enzyme's active site. The immobilization layer is prone to detachment, making it difficult for the sensor to conformally adhere to the skin. Signal acquisition is unstable, the device requires an external power supply, and issues such as sample evaporation and transmission delays make it difficult to achieve real-time and accurate monitoring.

Method used

A flexible bioelectrode was constructed by using an enzyme-immobilized strand conjugate combined with a circular DNA template to perform rolling circle amplification and self-assembled DNA hydrogel, immobilizing the enzyme in a three-dimensional network, and integrating sweat absorption and storage using polyacrylamide hydrogel. Self-powered monitoring was then achieved through an enzyme biofuel cell.

Benefits of technology

It achieves efficient directional immobilization and stable catalysis of enzymes, the flexible electrode does not detach under deformation, simplifies the device structure, provides self-powered sensing, and has high signal accuracy, making it suitable for home health monitoring.

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Abstract

The application discloses a kind of for sweat glucose concentration monitoring method, it is related to sweat monitoring field, comprising the following steps: step 1, enzyme-fixed chain conjugate synthesis: glucose dehydrogenase is reacted with bilirubin oxidase respectively with bifunctional crosslinking agent and is purified, obtain enzyme-crosslinking agent intermediate;It is clicked with azide modified DNA fixed chain chemistry reaction again, and after purification, obtain GDH-fixed chain and BOx-fixed chain conjugate;Step 2, preparation of circular DNA template: after annealing hybridization of phosphorylated template chain and primer chain, it is connected into ring by T4 DNA ligase;Step 3, rolling circle amplification self-assembly package enzyme DNA hydrogel;The present application uses super-flexible DNA hydrogel as flexible electrode material, realizes the efficient fixation and continuous stable catalysis of GDH and BOx, and constructs flexible sweat-based EBFC self-powered sensor as flexible bioelectrode, to realize continuous non-invasive blood glucose monitoring.
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