A device for detecting red blood cell folate

Abstract

The present application relates to the technical field of biological detection pretreatment device, and more particularly to a red blood cell folate pretreatment detection device, which comprises a magnetic stand, a reaction tube is placed on the magnetic stand, and a multi-gear magnetic separation mechanism for providing different magnetic field strengths in the magnetic adsorption stage, the washing stage and the resuspension stage is arranged on the magnetic stand, and the multi-gear magnetic separation mechanism comprises a lifting flat plate movably arranged in the magnetic stand. The lifting flat plate drives the cylindrical permanent magnet to approach or move away from the bottom of the reaction tube, and the three groups of rotating clamping blocks are matched to realize quick switching of gears. The strong magnetic gear makes the permanent magnet closely adhere to the bottom of the tube, and can quickly capture magnetic particles. The weak magnetic gear makes the permanent magnet maintain a proper distance, which can not only maintain adsorption but also avoid the agglomeration of magnetic beads. The zero magnetic gear makes the magnetic field basically disappear, which is convenient for resuspension. The three gears correspond to the core steps of pretreatment respectively, and the whole process is completed by one machine, so that the sample does not need to be transferred between different devices, and the detection efficiency and repeatability are effectively improved.

Description

Technical Field

[0001] This invention relates to the field of biological detection pretreatment devices, and more particularly to a red blood cell folic acid pretreatment detection device. Background Technology

[0002] Magnetic microparticle chemiluminescence immunoassay is one of the mainstream methods for detecting folic acid in erythrocytes. In its pretreatment process, a magnetic rack is used to perform magnetic separation of the magnetic microparticle complex, and the steps of targeted binding of magnetic microparticles to the target analyte, washing and removal of unbound impurities, and resuspension of magnetic microparticles are completed in sequence.

[0003] In existing technologies, the magnets of magnetic racks are usually fixedly installed inside the rack, and the magnetic field strength is constant and cannot be adjusted. However, in the actual operation of erythrocyte folic acid pretreatment, the magnetic adsorption stage requires a strong magnetic field to quickly capture magnetic particles, the washing stage requires a moderate magnetic field to keep the magnetic particles adsorbed at the bottom of the tube but to prevent them from being compressed and caking, thus affecting the washing effect, and the resuspension stage requires the magnetic field to be removed so that the magnetic particles can be evenly dispersed. Existing magnetic racks with fixed magnetic field strength cannot meet the different magnetic field strength requirements of the above three stages in the same device. It is often necessary to transfer samples between different devices or use manual shaking and other methods to assist resuspension, which is cumbersome and prone to introducing errors. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a red blood cell folic acid pretreatment detection device, which solves the technical problem that existing magnetic racks with fixed magnetic field strength cannot provide suitable magnetic field strengths for the three stages of magnetic adsorption, washing, and resuspension. It has the advantage of being able to switch between strong magnetic adsorption, weak magnetic washing, and zero magnetic resuspension within the same device.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a red blood cell folic acid pretreatment detection device, comprising a magnetic frame, on which reaction tubes are placed; a multi-level magnetic separation mechanism, used to provide progressively weakening magnetic field strengths in the magnetic adsorption stage, washing stage, and resuspension stage; the multi-level magnetic separation mechanism includes a lifting plate movably installed inside the magnetic frame; cylindrical permanent magnets, the same number as the reaction tubes and corresponding in position, are fixedly installed on the lifting plate, with adjacent cylindrical permanent magnets having opposite magnetic pole directions; fixed ear plates are symmetrically arranged on both sides of the lifting plate; and the magnetic frame has openings... The device is equipped with a clearance groove for the fixed ear plate to extend and slide up and down. The outer side of the magnetic frame is symmetrically equipped with guide slides to guide the fixed ear plate to move vertically up and down. The fixed ear plate is slidably sleeved on the guide slides. The outer side of the magnetic frame is equipped with a three-level limiting component that works with the fixed ear plate to lock the lifting plate at the strong magnetic adsorption height, weak magnetic washing height and zero magnetic re-suspension height respectively. The lifting plate drives the cylindrical permanent magnet to move closer to or away from the bottom of the reaction tube, realizing the switching of the three levels of strong magnetic adsorption, weak magnetic washing and zero magnetic re-suspension. It can meet the different magnetic field strength requirements of each stage of red blood cell folic acid pretreatment.

[0006] Preferably, the three-level limiting component includes three sets of rotating blocks arranged sequentially on the outside of the magnetic frame along the vertical direction. Each set of rotating blocks corresponds to the strong magnetic adsorption height, weak magnetic washing height, and zero magnetic re-suspension height. The three sets of rotating blocks correspond to three gear positions. Rotating the rotating block of the corresponding height to below the fixed ear plate can lock the lifting plate in that gear position. The operation is intuitive and the positioning is reliable.

[0007] Preferably, the magnetic frame is provided with an auxiliary positioning mechanism for aligning the bottoms of all reaction tubes. The auxiliary positioning mechanism includes an upper fixing plate and a middle guide plate fixedly installed on the magnetic frame, and a positioning slide plate movably installed inside the magnetic frame and horizontally removable. The positioning slide plate is located between the lifting plate and the middle guide plate. When the reaction tubes are inserted, the positioning slide plate can provide a uniform tube bottom support benchmark, so that the bottoms of reaction tubes of different specifications are on the same horizontal plane. After being removed, it will make room for the magnetic separation operation.

[0008] Preferably, the upper fixing plate has the same number of clamping through holes as the reaction tubes, and the inner wall of the clamping through holes is fitted with anti-slip rubber rings for clamping the upper end of the reaction tubes. The middle guide plate has the same number of guide holes as the reaction tubes. The anti-slip rubber rings provide holding force from the side to clamp the upper end of the reaction tubes, which can ensure that the reaction tubes are not pulled up when the positioning slide is pulled out. The guide holes are used to guide the reaction tubes to keep them vertical and prevent them from tilting when inserted.

[0009] Preferably, the magnetic frame has a side wall groove for guiding the horizontal pulling of the positioning slide plate, and an anti-mis-pulling block is movably installed on the magnetic frame. After the positioning slide plate is fully inserted, the anti-mis-pulling block will hang under its own weight, thereby blocking and limiting the positioning slide plate.

[0010] Preferably, the positioning slide plate is equipped with an integrated heating mechanism for constant-temperature incubation of the sample in the reaction tube. The positioning slide plate has a double-layer composite structure, including an upper heating panel and a lower heat insulation layer. The integrated heating mechanism includes a heating element embedded in the positioning slide plate. The tail of the positioning slide plate is provided with a metal contact piece, and the end of the side wall groove is provided with an electrical contact point that cooperates with the metal contact piece. When the positioning slide plate is pushed in, the metal contact piece will automatically contact and conduct electricity with the electrical contact point. At this time, the heating element will be energized to conduct constant-temperature incubation of the bottom of the reaction tube. When it is pulled out, the power will be automatically cut off, realizing the integration of positioning and heating functions.

[0011] Preferably, the heating element is a PTC heating element, and the heat insulation layer is aerogel felt or glass fiber insulation cotton. The PTC heating element has self-limiting temperature characteristics and can automatically maintain a constant temperature without additional temperature control circuit. The heat insulation layer can prevent heat from being transferred downward to the cylindrical permanent magnet, thereby preventing the magnet from demagnetizing due to heat.

[0012] Preferably, the upper end of the magnetic frame is provided with a swirling liquid addition mechanism for guiding the washing liquid to flow tangentially down the inner wall of the reaction tube. The swirling liquid addition mechanism includes a mounting base symmetrically arranged at the upper end of the magnetic frame. A liquid addition guide cover is hinged to the mounting base. The liquid addition guide cover has an inclined guide groove inside that corresponds one-to-one with the reaction tube. The outlet direction of the inclined guide groove is consistent with the tangential direction of the inner wall of the reaction tube. After the washing liquid flows out along the inclined guide groove, it will be ejected along the tangential direction of the inner wall of the reaction tube, forming a wall-attached swirling flow. This can avoid the liquid directly impacting the magnetic beads at the bottom of the tube and effectively reduce the loss of the magnetic beads.

[0013] Preferably, the upper surface of the liquid guide cover is provided with a funnel inlet corresponding to the starting end of each inclined diversion channel. The funnel inlet is enlarged in a funnel shape, which facilitates the positioning and dripping of the pipette tip, and the washing liquid can be smoothly introduced into the inclined diversion channel without precise alignment.

[0014] Preferably, the upper end of the magnetic frame is symmetrically provided with positioning support rods, and a magnetic positioning component is provided between the liquid filling guide cover and the positioning support rods to keep the cover closed. The magnetic positioning component will automatically attract when the liquid filling guide cover is closed, thereby ensuring the alignment accuracy between the outlet of the inclined diversion channel and the reaction tube.

[0015] By means of the above technical solution, the present invention provides a red blood cell folic acid pretreatment detection device, which has at least the following beneficial effects: 1. This invention uses a lifting plate to move a cylindrical permanent magnet closer to or further away from the bottom of the reaction tube, and with the help of three sets of rotating blocks, it can quickly switch between different magnetic settings. The strong magnetic setting keeps the permanent magnet close to the bottom of the tube, which can quickly capture magnetic particles. The weak magnetic setting keeps the permanent magnet at an appropriate distance, which can maintain adsorption and avoid magnetic beads from caking. The zero magnetic setting makes the magnetic field almost disappear, which is convenient for resuspension. The three settings correspond to the core steps of pretreatment, and the whole process can be completed in one machine. There is no need to transfer samples between different devices, which effectively improves detection efficiency and repeatability.

[0016] 2. In this invention, adjacent cylindrical permanent magnets are arranged with alternating N and S polarities, so that the magnetic field lines are evenly distributed at the bottom of the reaction tube. This can effectively avoid the problem of excessively strong or weak local magnetic fields caused by the same polarity arrangement. At the same time, the lifting plate is smoothly lifted by sliding cooperation between the fixed ear plate and the guide slide column, which can ensure that the magnetic field strength at the bottom of each reaction tube is consistent.

[0017] 3. This invention provides a uniform bottom support benchmark for all reaction tubes by setting a horizontally removable positioning slide plate. Operators only need to insert each reaction tube until it touches the upper surface of the positioning slide plate, so that the bottoms of reaction tubes of the same specification used in the same batch are automatically aligned to the same horizontal plane. This ensures that the distance between each cylindrical permanent magnet and the corresponding tube bottom is exactly the same when the lifting plate rises, which can guarantee the uniformity of the multi-hole magnetic separation effect.

[0018] 4. In this invention, the inner wall of the clamping through hole is fitted with an anti-slip rubber ring, which can clamp the upper end of the reaction tube from the side to provide holding force. This ensures that the reaction tube is not pulled up when the positioning slide is pulled out, and ensures that the bottom of the tube is suspended at a precise and consistent height. In addition, the anti-mistake block will automatically drop down to form a shield after the positioning slide is pushed in, which can prevent the positioning slide from being pulled out accidentally when the incubation is not completed, effectively reducing the risk of experimental failure caused by operational errors.

[0019] 5. This invention integrates the heating element directly into the positioning slide plate, enabling the positioning slide plate to perform the function of tube bottom alignment while also undertaking the function of constant temperature incubation. This achieves the integration of positioning and heating functions. When the positioning slide plate is pushed in, the heating element will automatically perform constant temperature incubation on the bottom of the reaction tube, and it will automatically cut off the power when it is pulled out. No additional operation is required, which simplifies the workflow and improves the operating efficiency.

[0020] 6. The heating element in this invention uses a PTC heating element, which has self-limiting temperature characteristics and can automatically maintain the set temperature without the need for an additional temperature control circuit. It has a simple structure and high reliability. The positioning slide adopts a double-layer composite structure. The upper heating panel is used to conduct heat evenly, and the lower insulation layer can effectively prevent heat from being transferred downwards. This can prevent the cylindrical permanent magnet below from being demagnetized by heat, thus ensuring the performance stability of the multi-position magnetic separation mechanism in long-term use.

[0021] 7. By setting up an inclined flow channel, the present invention can guide the washing liquid to the inlet of the reaction tube and eject it along the inner wall tangential direction to form a wall-adhering vortex. This allows the washing liquid to rotate down along the tube wall, first bypassing the magnetic beads that accumulate at the bottom of the tube from the side, and then slowly rising up for gentle cleaning. This can effectively avoid the direct impact of the liquid flow causing the magnetic beads to splash and be lost, and is especially suitable for red blood cell folic acid detection scenarios where the amount of magnetic beads is small.

[0022] 8. The funnel inlet of the present invention is widened in a funnel shape, which has a good guiding effect on the pipette tip. Even if the operator does not align it accurately, the liquid can be added smoothly, which reduces the difficulty of operation and the requirements for operator proficiency. In addition, the magnetic positioning component can ensure stable positioning after the liquid addition guide cover is closed, so as to ensure the alignment accuracy between the outlet of the inclined drainage channel and the reaction tube and ensure consistent liquid addition effect each time. Attached Figure Description

[0023] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figure 1 This is a three-dimensional schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the multi-stage magnetic separation mechanism in this invention; Figure 3 This is a schematic diagram of the lifting plate in this invention; Figure 4 This is a schematic diagram of the guide slide column in this invention; Figure 5 This is a schematic diagram of the auxiliary positioning mechanism in this invention; Figure 6 This is a schematic diagram of some structures in the present invention; Figure 7 This is a schematic diagram of the integrated heating mechanism in this invention; Figure 8 This is a schematic diagram of the swirl-type liquid dispensing mechanism in this invention; Figure 9 This is a cross-sectional schematic diagram of the liquid addition guide cap in this invention; Figure 10 This is a schematic diagram of the magnetic positioning component in this invention.

[0024] In the diagram: 1. Magnetic frame; 2. Reaction tube; 3. Multi-position magnetic separation mechanism; 301. Lifting plate; 302. Clearance groove; 303. Fixed ear plate; 304. Cylindrical permanent magnet; 305. Guide slide; 306. Three-stage limit assembly; 4. Auxiliary positioning mechanism; 401. Upper fixed plate; 402. Middle guide plate; 403. Clamping through hole; 404. Anti-slip rubber ring; 405. Guide round hole; 406. Fixed... 407. Anti-accidental withdrawal block; 5. Integrated heating mechanism; 501. Side wall groove; 502. Heating panel; 503. Heating element; 504. Electrical contact; 505. Metal contact piece; 506. Heat insulation layer; 6. Swirl-type liquid filling mechanism; 601. Mounting base; 602. Liquid filling guide cover; 603. Positioning support rod; 604. Funnel inlet; 605. Inclined diversion groove; 606. Magnetic positioning component. Detailed Implementation

[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] Example 1 Existing magnetic racks with fixed magnetic field strength cannot meet the differentiated magnetic field strength requirements of the magnetic adsorption, washing, and resuspension stages within the same device. This often necessitates transferring samples between different devices or using manual shaking to assist resuspension, which is cumbersome and prone to introducing errors. To address this technical deficiency in existing technologies, such as... Figures 1-6 As shown, this embodiment proposes a red blood cell folic acid pretreatment detection device that can switch between three levels of strong magnetic adsorption, weak magnetic washing and zero magnetic resuspension within the same device. The device includes a magnetic rack 1, on which a reaction tube 2 is placed. The magnetic rack 1 is equipped with a multi-level magnetic separation mechanism 3 for providing different magnetic field intensities during the magnetic adsorption stage, the washing stage and the resuspension stage.

[0027] Specifically, the multi-position magnetic separation mechanism 3 includes a lifting plate 301 movably installed within the magnetic frame 1. The lifting plate 301 is fixedly equipped with cylindrical permanent magnets 304, the same number and corresponding in position as the reaction tubes 2. The magnetic poles of adjacent cylindrical permanent magnets 304 are opposite in direction. Fixed ear plates 303 are symmetrically arranged on both sides of the lifting plate 301. The magnetic frame 1 has clearance grooves 302 for the fixed ear plates 303 to extend and slide vertically. Guide slides 305 are symmetrically arranged on the outer side of the magnetic frame 1 to guide the fixed ear plates 303 vertically. The fixed ear plates 303 are slidably fitted onto the guide slides 305. The outer side of the magnetic frame 1 is equipped with features that cooperate with the fixed ear plates 303 to lock the lifting plate 301 at strong magnetic adsorption heights. The three-level limiting component 306, which controls the three positions of strong magnetic adsorption, weak magnetic washing, and zero magnetic re-suspension, moves the cylindrical permanent magnet 304 closer to or further away from the bottom of the reaction tube 2 via the lifting plate 301. This allows for switching between the three positions, which can meet the different magnetic field strength requirements of each stage of red blood cell folic acid pretreatment. The three-level limiting component 306 includes three sets of rotating blocks arranged vertically on the outside of the magnetic frame 1. Each set of rotating blocks corresponds to the strong magnetic adsorption height, weak magnetic washing height, and zero magnetic re-suspension height, respectively. The three sets of rotating blocks correspond to the three positions. Rotating the rotating block of the corresponding height to below the fixed ear plate 303 locks the lifting plate 301 in that position. The operation is intuitive and the positioning is reliable.

[0028] As can be seen from the above, when the operator moves the lifting plate 301 upward, the fixed ear plate 303 will rise along the guide slide column 305, driving the cylindrical permanent magnet 304 closer to the bottom of the reaction tube 2.

[0029] When the lifting plate 301 reaches the strong magnetic adsorption height, the rotating block corresponding to the strong magnetic adsorption position can be rotated to lock under the fixed ear plate 303. At this time, the cylindrical permanent magnet 304 will be in close contact with the bottom of the reaction tube 2, providing a strong magnetic field, thereby quickly adsorbing the magnetic particles to the bottom of the tube.

[0030] During the washing stage, the lifting plate 301 is lowered to the weak magnetic washing height, and the rotating block corresponding to the weak magnetic washing position is rotated to lock it. At this time, the distance between the cylindrical permanent magnet 304 and the bottom of the reaction tube 2 will increase, the magnetic field will weaken, and the magnetic particles will still be adsorbed on the bottom of the tube but will not be pressed and clumped, which makes it easier for the washing liquid to fully clean the impurities.

[0031] During the resuspension stage, continue to lower the lifting plate 301 to the zero magnetic resuspension height or completely lower it, and rotate the rotating block corresponding to the zero magnetic resuspension position to lock it, so that the cylindrical permanent magnet 304 is away from the bottom of the reaction tube 2. At this time, the magnetic field basically disappears. Then, take out the reaction tube 2 and shake it, and the magnetic particles can be uniformly resuspended in the solution.

[0032] In this embodiment, the lifting plate 301 moves the cylindrical permanent magnet 304 closer to or further away from the bottom of the reaction tube 2, and three sets of rotating blocks enable rapid switching of the magnetic settings. The strong magnetic setting keeps the permanent magnet close to the bottom of the tube, which can quickly capture magnetic particles. The weak magnetic setting keeps the permanent magnet at an appropriate distance, which can maintain adsorption and prevent magnetic beads from caking. The zero magnetic setting makes the magnetic field almost disappear, which is convenient for resuspension. The three settings correspond to the core pretreatment steps, and the whole process can be completed in one machine without transferring samples between different devices, which effectively improves detection efficiency and repeatability. Moreover, in this embodiment, the adjacent cylindrical permanent magnets 304 are arranged with alternating N and N polarities, so that the magnetic lines of force are evenly distributed at the bottom of the reaction tube 2, which can effectively avoid the problem of excessively strong or weak local magnetic fields caused by the same polarity arrangement. At the same time, the lifting plate 301 slides with the guide slide 305 through the fixed ear plate 303, so that the lifting action is smooth and can ensure that the magnetic field strength at the bottom of each reaction tube 2 is consistent.

[0033] Example 2 To ensure that the bottoms of reaction tubes 2 of the same specification used in the same batch are automatically aligned to the same horizontal plane, thus guaranteeing the uniformity of the porous magnetic separation effect, based on Example 1, as follows: Figure 1 , Figure 2 , Figure 5 as well as Figure 6 As shown, in this embodiment, an auxiliary positioning mechanism 4 is provided on the magnetic frame 1 to align the bottoms of all reaction tubes 2. The auxiliary positioning mechanism 4 includes an upper fixing plate 401 and a middle guide plate 402 fixedly installed on the magnetic frame 1, and a positioning slide plate 406 movably installed inside the magnetic frame 1 and horizontally removable. The positioning slide plate 406 is located between the lifting plate 301 and the middle guide plate 402. When the reaction tubes 2 are inserted, the positioning slide plate 406 can provide a uniform tube bottom support reference, so that the bottoms of reaction tubes 2 of different specifications are on the same horizontal plane. After being pulled out, it will make room for the magnetic separation operation. The upper fixing plate 401 has the same number of clamping through holes 403 as the reaction tubes 2. The inner wall of the clamping through holes 403 An anti-slip rubber ring 404 is embedded to clamp the upper end of the reaction tube 2. The middle guide plate 402 has the same number of guide holes 405 as the reaction tube 2. The anti-slip rubber ring 404 clamps the upper end of the reaction tube 2 from the side to provide holding force, which can ensure that the reaction tube 2 is not pulled up when the positioning slide plate 406 is pulled out. The guide holes 405 are used to guide the reaction tube 2 to keep it vertical and prevent it from tilting when inserted. The magnetic frame 1 has a side wall groove 501 for guiding the horizontal pulling of the positioning slide plate 406. An anti-mistake block 407 is movably installed on the magnetic frame 1. After the positioning slide plate 406 is fully inserted, the anti-mistake block 407 will hang under its own weight, thereby blocking and limiting the positioning slide plate 406.

[0034] As can be seen from the above, before placing the reaction tube 2, the operator will first push the positioning slide plate 406 horizontally into the interior of the magnetic frame 1 along the side wall slide groove 501. After it is pushed into place, the anti-accidental withdrawal block 407 will automatically drop down under its own gravity, thereby blocking and limiting the front end of the positioning slide plate 406, which can prevent the positioning slide plate 406 from accidentally sliding out.

[0035] Subsequently, each reaction tube 2 is inserted sequentially through the clamping through hole 403 of the upper fixing plate 401, through the guide round hole 405 of the middle guide plate 402, and continues downward until the bottom of the reaction tube 2 touches the upper surface of the positioning slide plate 406. Since the bottom of all reaction tubes 2 is in contact with the positioning slide plate 406, the bottom of all reaction tubes 2 will be limited to the same horizontal plane.

[0036] After sample addition or incubation is completed, the operator will manually open the anti-accidental extraction block 407 and pull out the positioning slide plate 406 horizontally along the side wall groove 501. At this time, the upper end of the reaction tube 2 is clamped from the side by the anti-slip rubber ring 404, providing sufficient holding force, so the reaction tube 2 will not be lifted by the positioning slide plate 406.

[0037] After the positioning slide plate 406 is fully pulled out, the bottom of the reaction tube 2 is suspended in the air, which can make room for the subsequent magnetic separation operation. Moreover, since the bottom of all the reaction tubes 2 has been predetermined to be at the same height, the distance between each cylindrical permanent magnet 304 and the bottom of the corresponding reaction tube 2 will remain consistent when the lifting plate 301 rises, effectively ensuring the uniformity of the magnetic separation effect.

[0038] In this embodiment, a horizontally retractable positioning slide plate 406 provides a uniform bottom support reference for all reaction tubes 2. The operator only needs to insert each reaction tube 2 until it touches the upper surface of the positioning slide plate 406, so that the bottoms of reaction tubes 2 of the same specification used in the same batch are automatically aligned to the same horizontal plane. This ensures that when the lifting plate 301 rises, the distance between each cylindrical permanent magnet 304 and the corresponding tube bottom is exactly the same, which can guarantee the uniformity of the multi-hole magnetic separation effect. Moreover, in this embodiment, the inner wall of the clamping through hole 403 is fitted with an anti-slip rubber ring 404, which can clamp the upper end of the reaction tube 2 from the side to provide holding force. This can ensure that the reaction tube 2 is not pulled up when the positioning slide plate 406 is pulled out, and ensure that the tube bottom suspension height is accurately consistent. In addition, the anti-mistake block 407 will automatically droop down to form a shield after the positioning slide plate 406 is pushed in, which can prevent the positioning slide plate 406 from being accidentally pulled out before the incubation is completed, effectively reducing the risk of experimental failure caused by operational errors.

[0039] Example 3 To integrate positioning and heating functions, simplify the workflow, and improve operational efficiency, based on the above embodiments, such as... Figure 1 , Figure 6 as well as Figure 7As shown, this embodiment features an integrated heating mechanism 5 on the positioning slide plate 406 for constant-temperature incubation of the sample in the reaction tube 2. The positioning slide plate 406 has a double-layer composite structure, including an upper heating panel 502 and a lower insulation layer 506. The integrated heating mechanism 5 includes a heating element 503 embedded inside the positioning slide plate 406. The heating element 503 is a PTC heating element, and the insulation layer 506 is an aerogel felt or fiberglass insulation cotton. The PTC heating element has self-limiting temperature characteristics and can automatically incubate the sample without additional temperature control circuitry. The temperature is controlled by the insulation layer 506, which prevents heat from being transferred downwards to the cylindrical permanent magnet 304, thus preventing the magnet from demagnetizing due to heat. The tail of the positioning slide plate 406 is provided with a metal contact piece 505, and the end of the side wall slide groove 501 is provided with an electrical contact 504 that cooperates with the metal contact piece 505. When the positioning slide plate 406 is pushed in, the metal contact piece 505 will automatically contact and conduct with the electrical contact 504. At this time, the heating element 503 will be energized to keep the bottom of the reaction tube 2 constant temperature incubation. When it is pulled out, the power will be automatically cut off, realizing the integration of positioning and heating functions.

[0040] As can be seen from the above, when the positioning slide plate 406 is pushed into place along the side wall slide groove 501, the metal contact piece 505 at the tail of the positioning slide plate 406 will automatically make contact with the electrical contact 504 at the end of the side wall slide groove 501 to form a complete power supply circuit.

[0041] Subsequently, the current is transmitted through the metal contact 505 to the heating element 503 embedded inside the positioning slide plate 406. After the heating element 503 is powered on, it begins to generate heat. The heating element 503 uses a PTC heating element, whose resistance increases with the temperature. When the temperature reaches the Curie point, the resistance will increase sharply, automatically limiting the current and stabilizing the temperature within the set range, without the need for an additional temperature control circuit.

[0042] Next, heat is transferred from the heating element 503 to the bottom of each reaction tube 2 on the positioning slide plate 406, thereby incubating the sample inside the tube at a constant temperature. Moreover, the heat insulation layer 506 on the lower surface of the positioning slide plate 406 is made of aerogel felt or glass fiber heat insulation cotton, which can effectively prevent heat from being transferred downward, thereby ensuring that the cylindrical permanent magnet 304 below is not affected by heat and preventing the cylindrical permanent magnet 304 from irreversibly demagnetizing due to high temperature.

[0043] When the positioning slide plate 406 is pulled out, the metal contact 505 will separate from the power contact 504, the power supply circuit will be disconnected, and the heating element 503 will automatically cut off the power and stop heating, thus achieving energy saving and safety protection.

[0044] In this embodiment, the heating element 503 is directly integrated into the positioning slide plate 406, enabling the positioning slide plate 406 to perform the tube bottom alignment function while also undertaking the constant temperature incubation function. This achieves the integration of positioning and heating functions. When the positioning slide plate 406 is pushed in, the heating element 503 automatically performs constant temperature incubation on the bottom of the reaction tube 2, and automatically cuts off the power when it is pulled out, requiring no additional operation, simplifying the workflow and improving operational efficiency. Moreover, the heating element 503 in this embodiment uses a PTC heating element, which has self-limiting temperature characteristics and can automatically maintain the set temperature without the need for an additional temperature control circuit. The structure is simple and highly reliable. The positioning slide plate 406 adopts a double-layer composite structure. The upper heating panel 502 is used to uniformly conduct heat, while the lower heat insulation layer 506 can effectively prevent heat from being transferred downwards, which can prevent the cylindrical permanent magnet 304 below from being demagnetized by heat, ensuring the performance stability of the multi-level magnetic separation mechanism 3 in long-term use.

[0045] Example 4 To avoid splashing and loss of magnetic beads due to direct impact from the liquid flow during the washing operation, based on the above embodiments, such as Figure 1 and Figures 8-10 As shown, in this embodiment, a swirling liquid addition mechanism 6 is provided at the upper end of the magnetic frame 1 to guide the washing liquid to flow tangentially down the inner wall of the reaction tube 2. The swirling liquid addition mechanism 6 includes a mounting base 601 symmetrically arranged at the upper end of the magnetic frame 1. A liquid addition guide cover 602 is hinged to the mounting base 601. The liquid addition guide cover 602 has an inclined guide groove 605 corresponding to the reaction tube 2. The outlet direction of the inclined guide groove 605 is consistent with the tangential direction of the inner wall of the reaction tube 2. After the washing liquid flows out along the inclined guide groove 605, it will be ejected along the tangential direction of the inner wall of the reaction tube 2, forming a wall-attached swirling flow, which can avoid the liquid directly impacting the magnetic beads at the bottom of the tube. To effectively reduce magnetic bead loss, the upper surface of the liquid addition guide cover 602 is provided with a funnel inlet 604 corresponding to the starting end of each inclined diversion channel 605. The funnel inlet 604 is widened in a funnel shape to facilitate the positioning and dripping of the pipette tip. The washing liquid can be smoothly introduced into the inclined diversion channel 605 without precise alignment. The upper end of the magnetic frame 1 is symmetrically provided with positioning support rods 603. A magnetic positioning component 606 is provided between the liquid addition guide cover 602 and the positioning support rod 603 to keep the cover closed. The magnetic positioning component 606 will automatically attract when the liquid addition guide cover 602 is closed, thereby ensuring the alignment accuracy between the outlet of the inclined diversion channel 605 and the reaction tube 2.

[0046] As can be seen from the above, during the washing stage, the operator will close the liquid filling guide cover 602 downwards. At the same time, the magnetic positioning component 606 will automatically close, keeping the liquid filling guide cover 602 in a stable closed state. At this time, the outlet of each inclined drainage channel 605 will be precisely aligned with the tangential direction of the inner wall of the corresponding reaction tube 2.

[0047] Next, after the operator draws up the washing liquid with a pipette, he inserts the pipette tip into the corresponding funnel inlet 604. The funnel inlet 604 is widened in a funnel shape and has a guiding function. Even if the pipette tip is not precisely aligned, the washing liquid can still flow smoothly into the inclined drainage channel 605.

[0048] Subsequently, the operator will slowly press down the pipette piston, allowing the washing liquid to flow into the inclined guide channel 605 through the funnel inlet 604. Then, the washing liquid will be ejected from the outlet of the inclined guide channel 605 along the tangential direction of the inner wall of the reaction tube 2. Since the outlet direction is tangential to the inner wall of the reaction tube 2, the liquid will rotate and flow downward along the tube wall under the action of centrifugal force, forming a wall-adhering vortex.

[0049] The liquid swirling along the wall will bypass the magnetic beads that gather at the bottom of the tube from the side, spiral down along the tube wall to the bottom of the tube, and then rise from the bottom to gently wash the magnetic beads. Throughout the process, the liquid will not directly impact the magnetic bead belt, which can effectively prevent the magnetic beads from being scattered or lost.

[0050] After washing, the operator removes the waste liquid. At this time, reaction tube 2 is still in a weak magnetic field, and the magnetic bead remains in its original position at the bottom of the tube. The washing process can then be repeated as needed, or the liquid addition guide cover 602 can be flipped upwards for subsequent operations.

[0051] This embodiment, by setting an inclined drainage channel 605, guides the washing liquid to the inlet of the reaction tube 2 and ejects it tangentially along the inner wall to form a wall-adhering swirling flow. This allows the washing liquid to rotate down the tube wall, first bypassing the magnetic beads gathered at the bottom of the tube from the side, and then slowly rising up for gentle cleaning. This effectively avoids direct impact from the liquid flow, which could cause splashing and loss of magnetic beads. It is especially suitable for scenarios where the amount of magnetic beads is small, such as red blood cell folic acid detection. Moreover, the funnel inlet 604 in this embodiment is widened in a trumpet shape, which has a good guiding effect on the pipette tip. Even if the operator does not align it precisely, the liquid can be added smoothly, reducing the difficulty of operation and the requirements for operator proficiency. Furthermore, the magnetic positioning component 606 ensures stable positioning after the liquid addition guide cover 602 is closed, ensuring the alignment accuracy between the outlet of the inclined drainage channel 605 and the reaction tube 2, and guaranteeing consistent liquid addition results each time.

[0052] The provision of power is also common knowledge in this field. This invention is mainly used to protect mechanical devices, so the control method and circuit connection will not be explained in detail here.

[0053] It should be noted that, in this document, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0054] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for detecting folic acid pretreatment in erythrocytes, comprising a magnetic rack (1) on which a reaction tube (2) is placed, characterized in that: The magnetic rack (1) is equipped with a multi-level magnetic separation mechanism (3) to provide progressively weaker magnetic field strength in the magnetic adsorption stage, washing stage and resuspension stage. The multi-position magnetic separation mechanism (3) includes a lifting plate (301) movably installed in the magnetic frame (1). The lifting plate (301) is fixedly installed with cylindrical permanent magnets (304) that are the same number as the reaction tubes (2) and have corresponding positions. The magnetic poles of adjacent cylindrical permanent magnets (304) are opposite. Fixed ear plates (303) are symmetrically arranged on both sides of the lifting plate (301). The magnetic frame (1) is provided with a clearance vertical groove (302) for the fixed ear plates (303) to extend and slide up and down. The outer side of the magnetic frame (1) is symmetrically provided with guide slides (305) for guiding the fixed ear plates (303) to rise and fall vertically. The outer side of the magnetic frame (1) is provided with a three-level limiting component (306) that cooperates with the fixed ear plates (303) to lock the lifting plate (301) at the strong magnetic adsorption height, weak magnetic washing height and zero magnetic re-suspension height respectively.

2. The red blood cell folic acid pretreatment detection device according to claim 1, characterized in that: The three-level limiting component (306) includes three sets of rotating blocks arranged sequentially on the outside of the magnetic frame (1) along the vertical direction. Each set of rotating blocks corresponds to the strong magnetic adsorption height, weak magnetic washing height and zero magnetic re-suspension height position, respectively.

3. The red blood cell folic acid pretreatment detection device according to claim 1, characterized in that: The magnetic frame (1) is provided with an auxiliary positioning mechanism (4) for aligning the bottoms of all reaction tubes (2). The auxiliary positioning mechanism (4) includes an upper fixing plate (401) and a middle guide plate (402) fixedly installed on the magnetic frame (1), and a positioning slide plate (406) movably installed inside the magnetic frame (1) and horizontally removable. The positioning slide plate (406) is located between the lifting plate (301) and the middle guide plate (402).

4. The red blood cell folic acid pretreatment detection device according to claim 3, characterized in that: The upper fixing plate (401) has the same number of clamping through holes (403) as the reaction tube (2). The inner wall of the clamping through holes (403) is fitted with anti-slip rubber rings (404) for clamping the upper end of the reaction tube (2). The middle guide plate (402) has the same number of guide round holes (405) as the reaction tube (2).

5. The red blood cell folic acid pretreatment detection device according to claim 3, characterized in that: The magnetic frame (1) has a side wall groove (501) for guiding the horizontal pulling of the positioning slide plate (406), and an anti-mis-pulling block (407) is movably installed on the magnetic frame (1).

6. The red blood cell folic acid pretreatment detection device according to claim 5, characterized in that: The positioning slide (406) is provided with an integrated heating mechanism (5) for constant temperature incubation of the sample in the reaction tube (2). The positioning slide (406) is a double-layer composite structure, including an upper heating panel (502) and a lower heat insulation layer (506). The integrated heating mechanism (5) includes a heating element (503) embedded in the positioning slide (406). The tail of the positioning slide (406) is provided with a metal contact piece (505), and the end of the side wall groove (501) is provided with an electrical contact point (504) that cooperates with the metal contact piece (505).

7. The red blood cell folic acid pretreatment detection device according to claim 6, characterized in that: The heating element (503) is a PTC heating element, and the heat insulation layer (506) is an aerogel felt or glass fiber insulation cotton.

8. The red blood cell folic acid pretreatment detection device according to claim 1, characterized in that: The upper end of the magnetic frame (1) is provided with a swirling liquid adding mechanism (6) for guiding the washing liquid to flow tangentially down the inner wall of the reaction tube (2). The swirling liquid adding mechanism (6) includes a mounting base (601) symmetrically arranged on the upper end of the magnetic frame (1). A liquid adding guide cover (602) is hinged on the mounting base (601). The liquid adding guide cover (602) has an inclined guide groove (605) inside that corresponds one-to-one with the reaction tube (2). The outlet direction of the inclined guide groove (605) is consistent with the tangential direction of the inner wall of the reaction tube (2).

9. The red blood cell folic acid pretreatment detection device according to claim 8, characterized in that: The upper surface of the liquid addition guide cover (602) is provided with a funnel inlet (604) corresponding to the starting end of each inclined diversion channel (605).

10. The red blood cell folic acid pretreatment detection device according to claim 8, characterized in that: The upper end of the magnetic frame (1) is symmetrically provided with positioning support rods (603), and a magnetic positioning component (606) for maintaining the closed state is provided between the liquid filling guide cover (602) and the positioning support rods (603).

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