A tumor cell enrichment device with automatic countable tip change

Abstract

This invention relates to the field of biological experimental equipment technology, specifically an automatic counting and tip-changing tumor cell enrichment device, comprising: a pen body assembly and a detachable knife box cassette mounted on the outer shell of the pen body assembly; multiple knife boxes are slidably installed within the knife box cassette; each knife box contains several knife tips ready for use; the pen body assembly includes a shell assembly, internally housing a rotating assembly, a knife dispensing assembly, a knife unloading assembly, a control assembly, and a power module. The automatic counting and tip-changing tumor cell enrichment device provided by this invention can accommodate more knife boxes through the external knife box cassette, enabling batch preparation and rapid replacement of knife tips; through the rotating assembly, knife dispensing assembly, and knife unloading assembly, it achieves automatic tip replacement, reducing tip replacement time, improving operational efficiency, and lowering the risk of accidental injury and contamination during manual tip replacement; it also has an automatic counting function, avoiding operator counting errors and facilitating subsequent verification.

Description

Technical Field

[0001] This invention relates to the field of biological experimental equipment technology, specifically to an automatic counting and blade-changing tumor cell enrichment device. Background Technology

[0002] Before conducting molecular tests such as polymerase chain reaction (PCR) and gene sequencing, pathological sections are typically observed under an optical microscope to locate tumor areas. Tumor cells are then enriched to extract nucleic acids, reducing interference from normal cell nucleic acids and ensuring the accuracy and reliability of gene testing results. Existing tumor cell enrichment tools often use disposable syringe needles or blades. To avoid contamination, new enrichment tools are needed when changing samples. When testing large sample volumes, using needles as tumor cell enrichment tools is time-consuming due to the time spent disassembling syringe packaging each time; because the needle is empty, tumor tissue can sometimes get stuck inside the syringe, causing sample loss; and because the needle area is small, it cannot effectively adhere to the slide when scraping tissue, resulting in time-consuming operations and significant cell loss. When using blades as tumor cell enrichment tools, the lack of a matching grip for the blade makes it easy for operators to injure themselves, especially for enriching small tissues, where blades are extremely inconvenient to use.

[0003] Chinese patent CN219314932U discloses a tool for manually enriching tumor cells, comprising: a blade head with multiple blades of different areas for scraping cells of different sizes; and a handle connected to the blade head for fixing it in place. While this tool provides a grip to hold the blades, it cannot efficiently replace the blade head. Frequent manual blade head replacement is inconvenient and time-consuming, and operators are prone to hand injuries during replacement, potentially increasing the risk of sample contamination. Furthermore, the grip lacks a counting function, requiring manual verification of the number of blades used and the number of samples scraped.

[0004] Therefore, it is crucial to provide a tumor cell enrichment device that can avoid direct contact between the hand and the blade, facilitate quick blade replacement, and automatically count tumor cells. Summary of the Invention

[0005] The purpose of this invention is to provide an automatic counting and changing device for tumor cell enrichment, in order to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] An automatic counting and blade-changing tumor cell enrichment device includes: a pen body assembly and a blade box detachably mounted on the housing of the pen body assembly; multiple blade boxes are slidably installed in the blade box detachably, and the blade box located at the bottom is accurately aligned with a target position on the housing of the pen body assembly; the blade box contains a number of blades to be used.

[0008] The pen body assembly includes a housing assembly, which has corresponding slots and cavities inside for mounting the operating assembly, the blade extension assembly, the blade removal assembly, the control assembly, and the power module; the housing assembly also has necessary electrical wiring to ensure the circuit connectivity of the internal electronic components.

[0009] The operating component is used to automatically transfer the blade from the outside of the pen body assembly housing to the inside; the blade dispensing component is used to control the blade to automatically slide down from the inside of the pen body assembly to the bottom to expose the blade tip and fix it; the blade unloading component is used to control the blade to automatically detach from the bottom of the pen body assembly; the control component includes a display control component for controlling, calculating and displaying the current number of blades in use; the power module provides power to the entire device.

[0010] Preferably, the housing assembly includes a front shell, a back shell, a bottom shell, and a top cover; a blade inlet is provided at the bottom of the back shell; a running cavity is provided inside the housing corresponding to the blade inlet; a blade sliding cavity is provided at the lower part of the running cavity; the central axis of the blade sliding cavity coincides with the central axis of the pen body assembly; a first baffle groove is provided between the bottom of the running cavity and the top of the blade sliding cavity; and a second baffle groove is provided at the bottom of the blade sliding cavity.

[0011] Preferably, the upper end of the cutter head is provided with a frustum-shaped protrusion, the top surface of which is made of a conductive metal surface, and the rest is insulated.

[0012] Preferably, the operating component includes an operating module and a operating motor connected by a central axis;

[0013] The operating module is rotatably mounted in the operating cavity along its own axis. A cutter head groove is eccentrically formed on the circumferential side wall of the operating module. The groove opening diameter matches the maximum cross-sectional diameter of the cutter head. An operating switch is provided on the upper inner side of the cutter head groove. The operating switch is connected to the operating motor circuit.

[0014] The output end housing of the motor is provided with a running limit block, and the rotating rod of the output end is provided with a protrusion. The running limit block is a semi-circular ring structure that surrounds the output end and matches the protrusion.

[0015] When the operating module is in its initial state, the opening of the cutter slot is directly opposite the cutter inlet. When the cutter is pushed into the cutter slot, the switch on the operating switch is pressed, causing it to retract into the side wall of the cutter slot. The operating motor rotates clockwise, causing the cutter slot and the cutter within it to rotate to the axis of the pen body assembly, directly opposite the top opening of the cutter slide cavity. When the cutter disengages from the cutter slot, the switch on the operating switch pops out again from the side wall of the cutter slot, the operating motor rotates counterclockwise, and the opening of the cutter slot is once again directly opposite the cutter inlet.

[0016] Preferably, the blade ejection assembly includes a blade ejection button, and the tail end of the blade ejection button is vertically provided with a two-stage linkage structure;

[0017] The first-stage linkage structure includes a first outgoing blade and a first baffle. The top end of the first outgoing blade is fixedly connected to the tail end of the outgoing blade button, and the bottom end of the first outgoing blade is fixedly connected to the tail end of the first baffle. The first baffle can slide horizontally in the first baffle groove. When the outgoing blade button is in the initial state, the first baffle blocks the channel between the blade head groove and the blade head slide cavity. When the outgoing blade button is pressed, the first baffle moves backward to connect the channel between the blade head groove and the blade head slide cavity.

[0018] The second-stage linkage structure includes a second blade extension rod and a second baffle; the top end of the second blade extension rod is fixedly connected to the bottom end of the first baffle, and the bottom end of the second blade extension rod is fixedly connected to the tail end of the second baffle; the second baffle can move horizontally in the groove of the second baffle; the first blade extension rod and the second blade extension rod are always perpendicular to the pressing and rebound direction of the blade extension button;

[0019] When the cutter eject button is pressed, the second baffle moves backward to allow the cutter head that has fallen above to pass through; when the cutter eject button springs back to its initial state, the bottom surface of the second baffle abuts against the top surface of the frustum-shaped protrusion at the upper end of the fallen cutter head to prevent the cutter head in use from being pushed back.

[0020] Preferably, a first electrode contact is provided at the upper end of the second baffle groove, a second electrode contact is provided on the top surface of the second baffle, and a third electrode contact is provided on the bottom surface of the second baffle;

[0021] When the second baffle is in its initial state, the first electrode contact and the second electrode contact are connected; when the second baffle moves backward, the first electrode contact and the second electrode contact are disconnected; when the frustum-shaped metal top surface of the cutter head abuts against the bottom surface of the second baffle, the third electrode contact is in a conductive state; when the bottom surface of the second baffle is suspended, the third electrode contact is in an open circuit state.

[0022] Preferably, the blade ejection assembly further includes a blade ejection switch, wherein when the blade ejection switch is in the closed state, its on / off switch prevents the first blade ejection rod from moving backward; the blade ejection switch is connected to the display control component circuit through the first electrode contact, the second electrode contact, and the third electrode contact;

[0023] When the first electrode contact and the second electrode contact are in a conductive connection, and simultaneously when the third electrode contact is in a conductive state, the tool dispensing switch is in a closed state, and the tool dispensing button is locked and cannot be pressed; when the third electrode contact is in a broken circuit state, the tool dispensing switch is in an open state, and the tool dispensing button can be pressed; after the tool dispensing button is pressed, the first electrode contact and the second electrode contact are disconnected, and the tool dispensing switch remains in an open state;

[0024] When the blade-dispensing switch is in the open state, the operating motor stops working; when the blade-dispensing switch is in the closed state, the operating motor resumes working; when the third electrode contact changes from the conducting state to the disconnecting state once, the calculator in the display control component counts once and displays the counting result on the display screen; the count is reset to zero after the power module is turned off, and starts counting from zero after the power module is turned on.

[0025] Preferably, the unloading assembly includes an unloading button, and the button seat of the unloading button has a first outgoing rod through groove that matches the first outgoing rod; the first outgoing rod passes through the first outgoing rod through groove and can move back and forth in the first outgoing rod through groove;

[0026] The unloading button is located at the other end of the first unloading rod through groove, and the unloading rod is always perpendicular to the pressing and rebound direction of the unloading button. The bottom end of the unloading rod is fitted with an unloading pull rod, and the other end of the unloading pull rod away from the unloading rod is symmetrically fitted with two sets of scissor structures. The bottom ends of the two sets of scissor structures are surrounded by a bottom groove.

[0027] When the unloading button is pressed, the unloading rod drives the unloading pull rod to move outward. The unloading pull rod pulls the two sets of scissor fork structures to rotate synchronously, causing the bottom groove to separate and open, thereby allowing the blade head, which was originally located in the bottom groove, to slide out of the housing of the pen body assembly.

[0028] Preferably, the two sets of scissor lift structures include two sets of symmetrically arranged forks, each set of forks rotating in an "X" shape around two central fixed axes, the two central fixed axes being fixedly connected to the bottom shell; the top ends of the two forks in the same set are respectively provided with a first movable shaft and a second movable shaft; the first movable shaft and the second movable shaft are simultaneously provided with a balance groove rod; the bottom ends of the two sets of opposing forks are respectively fixedly provided with a rocker shaft, each of the two rocker shafts having a semi-groove in the middle, the two semi-grooves forming the bottom groove when they are in contact.

[0029] Preferably, the knife box includes a knife box shell, and a knife box spring and a push plate are disposed inside the knife box shell; the knife box spring pushes the push plate to slide inside the knife box shell, pushing out the knife head; a knife box limiting block is disposed at the middle of the side of the push plate, and the knife box limiting block slides out of the outer side of the knife box shell; a knife box limiting groove is disposed on the box plate of the knife box, and the knife box limiting block can slide within the knife box limiting groove.

[0030] Compared with the prior art, the beneficial effects of the present invention are:

[0031] 1. The tumor cell enrichment device with automatic counting and blade changing provided by the present invention provides standardized special blades, which are more suitable for tumor cell enrichment work; at the same time, through the blade box and blade box combination structure, the function of batch preparation and rapid replacement of blades can be realized, which is suitable for use scenarios where the number of tumor cell samples to be enriched is large and frequent blade replacement is required.

[0032] 2. The tumor cell enrichment device with automatic counting and blade changing provided by the present invention realizes the function of rapid blade changing without hand contact through the operating component, the blade dispensing component and the blade unloading component, which greatly improves the efficiency of blade changing and avoids accidental hand injury and blade contamination.

[0033] 3. The tumor cell enrichment device with automatic counting and blade changing provided by the present invention reasonably avoids operational errors during operation through a multi-layered anti-accidental touch mechanism.

[0034] 4. The tumor cell enrichment device with automatic counting and blade changing provided by the present invention has an automatic counting function, so the operator does not need to be distracted by counting during use, thus avoiding counting errors and improving work efficiency. Attached Figure Description

[0035] Figure 1 This is an external view of an automatic counting and changing head tumor cell enrichment device;

[0036] Figure 2 An exploded view of an automatic counting and cutting head changing device for enriching tumor cells;

[0037] Figure 3 Exploded view of the shell assembly structure;

[0038] Figure 4 This is a schematic diagram of the internal structure of the front shell;

[0039] Figure 5 This is a schematic diagram of the rear view of the back shell structure;

[0040] Figure 6 This is an exterior view of the knife box / case.

[0041] Figure 7 This is a cross-sectional view of the knife box in its packaged state.

[0042] Figure 8 A diagram illustrating the knife box sliding into the knife box holder during use;

[0043] Figure 9 This is an external view of the operating module;

[0044] Figure 10 A detailed structural diagram of the output end of the motor;

[0045] Figure 11 This is a schematic diagram showing the docking status of the operating module and the tool box;

[0046] Figure 12 A schematic diagram showing the status of the rotating module transporting the cutting head;

[0047] Figure 13 This is a schematic diagram of the combined structure of the tool ejection assembly, tool unloading assembly, and operating module.

[0048] Figure 14 This is a schematic diagram of the operating cavity structure at point A of the front shell.

[0049] Figure 15 This is a schematic diagram of the cutter head sliding cavity structure at point B of the front shell;

[0050] Figure 16 Detailed diagram of the positioning groove structure at point C on the back cover;

[0051] Figure 17 This is a schematic diagram of the limiting structure of the blade ejection assembly at point D;

[0052] Figure 18 This is a schematic diagram of the connection structure between the tool delivery assembly and the tool unloading assembly at point D.

[0053] Figure 19 This is a schematic diagram showing the relative position of the cutting head and the cutting component when the operating module at point E is operating;

[0054] Figure 20 This is a top view showing the relative positions of the tool ejection assembly and the tool unloading assembly at point F in the initial state.

[0055] Figure 21 This is a top view showing the relative positions of the tool ejection assembly and the tool unloading assembly at point F in the initial state.

[0056] Figure 22 This is a cross-sectional view showing the relative position of the unloading assembly at point F and the bottom shell in the unloading state.

[0057] In the diagram: 1. Pen body assembly; 101. Front shell; 1011. Operating cavity; 1012. Blade ejection chamber; 1012a. First blade ejection lever limiting groove; 1012b. First baffle groove; 1012c. Second blade ejection lever limiting groove; 1012d. Second baffle groove; 1013. Blade unloading chamber; 1013a. Blade unloading lever limiting groove; 1014. Blade tip sliding cavity; 102. Back shell; 021. Tool holder slide groove; 1022. Tool holder slide groove; 1023. Tool head inlet; 103. Bottom shell; 104. Top cover; 11. Operating module; 111. Motor hole; 112. Tool head slot; 113. Operating switch; 12. Tool ejection assembly; 121. Tool ejection button; 122. First tool ejection rod; 123. First baffle; 124. Second tool ejection rod; 125. Second baffle; 13. Tool unloading assembly; 131. Tool unloading button; 131a. First tool delivery bar slot; 132. Tool unloading bar; 133. Tool unloading pull rod; 134. Fork bar; 135. First movable shaft; 136. Second movable shaft; 137. Central fixed shaft; 138. Balance groove rod; 139. Swing shaft; 14. Bottom groove; 2. Tool box; 201. Tool box slider; 202. Tool box limiting groove; 3. Tool box; 301. Tool box shell; 302. Encapsulation piece; 303. Push plate; 304. Tool box limiting block; 305. Tool box spring; 306. Tool head; 4. Rotation motor; 41. Output end; 42. Rotation limiting block; 5. Display control assembly; 6. Tool delivery switch; 7. Power module; 8a. First electrode contact; 8b. Second electrode contact; 8c. Third electrode contact. Detailed Implementation

[0058] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

[0059] like Figure 1 As shown, the present invention provides an automatic counting and tip-changing tumor cell enrichment device, including a pen body assembly 1 and a detachable blade holder 2 mounted on the housing of the pen body assembly 1. Multiple blade holders 3 are slidably mounted within the blade holder 2, with the bottom blade holder 3 precisely aligned with a target position on the housing of the pen body assembly 1. Several blade tips 306 are encapsulated within the blade holder 3.

[0060] like Figure 2 As shown, the pen body assembly 1 includes a housing assembly. The housing assembly has corresponding slots and cavities inside for installing the operating assembly, the blade extension assembly 12, the blade removal assembly 13, the control assembly, and the power module 7. The housing assembly also has necessary electrical wiring pathways inside to ensure the circuit connectivity of the internal electronic components.

[0061] The rotating component is used to automatically transfer the blade 306 from the outside of the pen body assembly 1 housing to the inside; the blade extension component 12 is used to control the blade 306 to automatically slide down from the inside of the pen body assembly 1 to the bottom to expose the blade tip and fix it for easy use by the operator; the blade removal component 13 is used to control the blade 306 to automatically detach from the bottom of the pen body assembly 1, so that the operator's hands do not need to directly contact the blade 306 to complete the replacement operation.

[0062] The control components include a display control component 5, which has a display screen for controlling, calculating, and displaying the current number of cutter heads 306 in use. The power module 7 supplies power to the entire device and can operate in battery mode or charging mode. If charging mode is used, a corresponding charging port is provided in the cavity housing of the power module 7.

[0063] Specifically, such as Figure 3 As shown, the housing assembly includes a front shell 101, a back shell 102, a bottom shell 103, and a top cover 104. The front shell 101 and back shell 102 together form the main body of the pen. The upper end of the main body has two opposing button holes. Below the button holes, the front shell 101 has a screen opening, and the display control component 5 is installed in the cavity behind the screen opening. Its display screen extends out of the housing through the screen opening for easy observation by the operator. The rotating component is rotatably installed in the cavity below the screen opening. The bottom shell 103 is installed on the lower outer periphery of the main body, and a through hole is opened at the center of the bottom of the bottom shell 103, allowing the blade 306 to slide out from inside the main body. The bottom of the top cover 104 is electrically connected to the power module 7, and a main power switch is located on the outside of the top cover 104. This switch can be a touch switch or a push-button switch; touching / pressing with a finger turns on the power, and quickly touching / pressing twice in succession turns off the power.

[0064] After leaving the factory, the housing components need to be painted or wrapped to cover the seams, improve the appearance, and ensure safety and insulation.

[0065] like Figure 5 and Figure 16 As shown, a knife box slide groove 1021 is provided on the outer wall of the back shell 102, and a knife box slide groove 1022 is opened at the bottom end of the knife box slide groove 1021, and a knife head inlet 1023 that penetrates the back shell 102 is opened here.

[0066] like Figure 6As shown, the knife box 2 has a rectangular box structure, including two box plates on both sides and a connecting plate connecting the two box plates. A knife box slider 201 is provided on the outer side of the two box plates away from the connecting plate. The knife box slider 201 matches the knife box groove 1021. When the knife box slider 201 slides to the bottom of the knife box groove 1021, the knife box 2 can be stably installed on the back shell 102. The cross-section of the knife box 3 matches the cross-section of the knife box 2. Through the knife box 2, the knife box 3 can slide downwards against the outer wall of the back shell 102.

[0067] like Figure 7 As shown, the blade holder 3 includes a blade holder housing 301, with an outlet on only one side for the blade head 306 to be pushed out. Several blade holder springs 305 are arranged inside the blade holder housing 301 away from the outlet; the central axis of each blade holder spring 305 is perpendicular to the plane of the outlet; one end of each blade holder spring 305 away from the outlet is fixedly connected to the inner wall of the blade holder housing 301, and the other end is fixedly connected to a push plate 303; under the push of the blade holder springs 305, the push plate 303 can slide to the outlet of the blade holder housing 301. A blade holder limiting block 304 is provided in the middle of the side of the push plate 303; a sliding groove is formed in the blade holder housing 301 along the sliding direction of the push plate 303; the blade holder limiting block 304 protrudes through the sliding groove to the outer side of the blade holder housing 301. The outlet of the unused knife box 3's outer shell 301 is sealed by a sealing sheet 302. Several knife heads 306 are arranged sequentially between the sealing sheet 302 and the push plate 303. The upper end of the knife head 306 is provided with a frustum-shaped protrusion, and the lower end is the blade. The top surface of the frustum-shaped protrusion at the upper end of the knife head 306 is made of conductive metal material, and the other parts of the surface are insulated. When the sealing sheet 302 is removed, the knife head 306 can slide towards the opening of the knife box outer shell 301 under the push of the push plate 303 until all the knife heads 306 are completely pushed out.

[0068] like Figure 8 As shown, a knife box limiting groove 202 matching the knife box limiting block 304 is provided on one side of the box plate of the knife box 2. The knife box limiting groove 202 is generally "L" shaped. When the knife box 3 is pushed into the knife box 2, the knife box limiting block 304 slides down along the "L" shaped longitudinal groove of the knife box limiting groove 202 with the knife box 3. As new knife boxes 3 are continuously put into the knife box 2, the bottom knife box 3 will be gradually pushed to the bottom of the knife box 2. At this time, the opening of the bottom knife box 3 is directly opposite the knife head inlet 1023 of the back shell 102, and the knife box limiting block 304 of the knife box 3 can slide in the transverse knife box limiting groove 202. After all the blades 306 in the bottom blade box 3 are pushed out, the blade box limiting block 304 is located at the starting position of the blade box slide groove 1022 of the back shell 102. At this time, the new blade box 3 is pushed in from the top of the blade box box 2, which can make the bottom blade box 3 detach from the inside of the blade box box 2, so as to achieve the purpose of replacing the blade box 3.

[0069] The tool box limiting block 304 can determine the relative position of the tool box 3 being pushed into the tool box 2, and can also determine the remaining state of the tool head 306 in the bottom tool box 3, so as to facilitate timely replacement of the new tool box 3.

[0070] like Figure 4 , Figures 9-12 As shown, a running cavity 1011 is provided inside the housing corresponding to the blade inlet 1023 at the bottom of the back cover 102, and a blade slide cavity 1014 is provided at the lower part of the running cavity 1011; the central axis of the blade slide cavity 1014 coincides with the central axis of the pen body assembly 1.

[0071] The operating assembly includes a rotating module 11 and a rotating motor 4 connected by a central axis. The rotating module 11 is rotatably disposed within a rotating cavity 1011 along its own axis. A motor hole 111 is provided on the top of the rotating module 11, and the motor hole 111 is axially connected to the output end 41 of the rotating motor 4. A cutter head groove 112 is eccentrically formed on the circumferential side wall of the rotating module 11. The groove diameter of the cutter head groove 112 matches the maximum cross-sectional diameter of the cutter head 306. When the rotating module 11 is in the initial state, the groove opening of the cutter head groove 112 is directly opposite the cutter head inlet 1023, and the dimensions of the cutter head groove 112 and the cutter head inlet 1023 match the cutter head 306, so that the cutter head 306 outside the cutter head inlet 1023 can be pushed into the cutter head groove 112. An operating switch 113 is installed on the upper inner side of the cutter head groove 112, and the operating switch 113 is electrically connected to the operating motor 4. When the cutter head 306 is pushed into the cutter head groove 112, the frustum-shaped protrusion at the upper end of the cutter head 306 presses against the switch 113, causing it to retract into the side wall of the cutter head groove 112. The operating motor 4 rotates clockwise, driving the operating module 11 to rotate clockwise along the axis, so that the cutter head groove 112 and the cutter head 306 therein are rotated clockwise. Rotate from the cutter head inlet 1023 to the axis of the pen body assembly 1, directly opposite the top opening of the cutter head slide cavity 1014; after the cutter head 306 disengages from the cutter head groove 112, the switch of the operating switch 113 pops out again from the side wall of the cutter head groove 112, the operating motor 4 rotates counterclockwise, driving the operating module 11 to rotate counterclockwise along the axis, and the groove of the cutter head groove 112 is once again directly opposite the cutter head inlet 1023, waiting for the next cutter head 306 to be pushed in.

[0072] The on / off switch 113 contacts the inclined surface of the frustum-shaped protrusion at the upper end of the cutter head 306, creating a downward pushing force on the cutter head 306. When there is no obstruction at the bottom of the cutter head 306, the on / off switch 113 assists in pushing the cutter head 306, making it easier for it to slide into the cutter head slide cavity 1014 below.

[0073] like Figure 10As shown, a running limit block 42 is provided on the output end housing of the running motor 4, and a protrusion is provided on the rotating rod of the output end 41. The running limit block 42 is a semi-circular ring structure that surrounds the output end 41 and matches the protrusion, so that the output end 41 can only rotate within the half-circle range that is not blocked by the running limit block 42, and the cutter head groove 112 can accurately stop at the target position.

[0074] like Figure 4 , Figures 13-15 , Figures 17-19 As shown, the front shell 101 and the back shell 102 together form a blade ejection cavity 1012 and a blade removal cavity 1013. The blade ejection cavity 1012 and the blade removal cavity 1013 are respectively provided with blade ejection springs and blade removal springs with opposite ejection directions, so that the button 121 of the blade ejection assembly 12 and the button 131 of the blade removal assembly 13 can extend out of the shell of the pen body assembly 1 in opposite directions, and can spring back to their original position after being pressed and released.

[0075] Specifically, the blade ejection assembly 12 includes a blade ejection button 121, with a two-stage linkage structure vertically arranged at its tail end. The first-stage linkage structure includes a first blade ejection rod 122 and a first baffle 123; the top end of the first blade ejection rod 122 is fixedly connected to the tail end of the blade ejection button 121, and the bottom end of the first blade ejection rod 122 is fixedly connected to the tail end of the first baffle 123. The second-stage linkage structure includes a second blade ejection rod 124 and a second baffle 125; the top end of the second blade ejection rod 124 is fixedly connected to the bottom of the first baffle 123, and the bottom end of the second blade ejection rod 124 is fixedly connected to the tail end of the second baffle 125. The first blade ejection rod 122 and the second blade ejection rod 124 are always perpendicular to the pressing and rebound direction of the blade ejection button 121.

[0076] Furthermore, the front shell 101 and the back shell 102 together form a first tool extension rod limiting groove 1012a and a second tool extension rod limiting groove 1012c that respectively match the first tool extension rod 122 and the second tool extension rod 124; the first tool extension rod limiting groove 1012a and the second tool extension rod limiting groove 1012c keep the first tool extension rod 122 and the second tool extension rod 124 in the same vertical plane and move back and forth synchronously; a first baffle groove 1012b is provided between the bottom of the operating cavity 1011 and the top of the cutter head sliding cavity 1014; the first baffle 123 can slide and translate in the first baffle groove 1012b; a second baffle groove 1012d is provided at the bottom of the cutter head sliding cavity 1014; the second baffle 125 can slide and translate in the second baffle groove 1012d.

[0077] When the cutter eject button 121 is in its initial state, the first baffle 123 is located at the bottom of the operating cavity 1011, blocking the channel between the cutter head groove 112 and the cutter head slide cavity 1014. When the cutter eject button 121 is pressed, the first baffle 123 moves backward in the first baffle groove 1012b, connecting the channel between the cutter head groove 112 and the cutter head slide cavity 1014, allowing the cutter head 306 to slide from the cutter head groove 112 into the cutter head slide cavity 1014. At this time, the second baffle 125 moves backward synchronously to allow the cutter head 306 that has fallen above to pass through. When the cutter eject button 121 rebounds and returns to its initial state, the bottom surface of the second baffle 125 abuts against the top surface of the frustum-shaped protrusion at the upper end of the fallen cutter head 306, preventing the cutter head 306 in use from being pushed back.

[0078] Furthermore, a first electrode contact 8a is provided at the upper end of the groove of the second baffle 1012d, and a second electrode contact 8b is provided on the top surface of the second baffle 125. When the second baffle 125 is in the initial state, the first electrode contact 8a and the second electrode contact 8b are connected. When the second baffle 125 moves backward, the first electrode contact 8a and the second electrode contact 8b are disconnected. A third electrode contact 8c is provided on the bottom surface of the second baffle 125. When the frustum-shaped metal top surface of the cutter head 306 abuts against the bottom surface of the second baffle 125, the third electrode contact 8c is in a conductive state. When the bottom surface of the second baffle 125 is suspended, the third electrode contact 8c is in an open circuit state.

[0079] The blade ejection assembly 12 also includes a blade ejection switch 6. When the blade ejection switch 6 is closed, its on / off switch prevents the first blade ejection rod 122 from moving backward. The blade ejection switch 6 is connected to the display control assembly 5 circuit through the first electrode contact 8a, the second electrode contact 8b, and the third electrode contact 8c.

[0080] When the first electrode contact 8a and the second electrode contact 8b are connected, and simultaneously when the third electrode contact 8c is in a conductive state, the tool dispensing switch 6 is in a closed state, and the tool dispensing button 121 is locked and cannot be pressed. This is because when the third electrode contact 8c is in a conductive state, it indicates that a tool head 306 is in use, and the tool dispensing switch 6 is closed to prevent the tool dispensing button 121 from being accidentally pressed. When the third electrode contact 8c is in an open state, it indicates that no tool head 306 is in use, the tool dispensing switch 6 is in a closed state, and the tool dispensing button 121 can be pressed.

[0081] When the blade ejection button 121 is pressed, the first electrode contact 8a and the second electrode contact 8b are disconnected, and the blade ejection switch 6 remains open, allowing the blade head 306 to slide smoothly inside the pen body. When the blade ejection switch 6 is open, the motor 4 stops working to prevent excessive rotation of the blade head 306 from causing blockage of the internal channels of the pen body. When the blade ejection switch 6 is closed, the motor 4 resumes working.

[0082] In this invention, the starting of the motor 4 relies on two judgment mechanisms: the first judgment mechanism is the operation switch 113 inside the blade slot 112; when the operation switch 113 is pressed, it indicates that there is a blade 306 inside the blade slot 112, and at this time the blade slot 112 should rotate towards the central axis of the pen body; when the operation switch 113 is not pressed, it indicates that there is no blade 306 inside the blade slot 112, and at this time the blade slot 112 should rotate towards the blade inlet 1023. The second judgment mechanism is the state of the blade dispensing switch 6; when the blade slot 112 has not rotated to the position of the central axis of the pen body, pressing the blade dispensing button 121 is considered an "abnormal" operation, and the motor 4 stops working to avoid operator error causing abnormal damage to the blade 306 or excessive operation of the blade 306 leading to blockage of the internal channel of the pen body.

[0083] The above-mentioned judgment mechanism works as follows: During the installation of the blade box 3, the operating switch 113 is in the pop-up state, and the blade slot 112 is directly opposite the blade inlet 1023. At this time, there is no blade 306 stored inside the pen body. Although the blade eject button 121 can be pressed, the blade 306 will not be exposed. When the blade box 3 is pushed to the bottom of the blade box cassette 2, the blade box spring 305 inside the blade box 3 pushes the push plate 303 to push the blade 306 into the blade slot 112. Pressing the operating switch 113 causes the operating motor 4 to rotate clockwise. After the blade slot 112 drives the blade 306 to rotate to the central axis of the pen body, pressing the blade eject button 121 at this time will allow the blade 306 to slide down and be exposed. Before the blade slot 112 drives the blade 306 to rotate to the central axis of the pen body, pressing the blade release button 121 too early is an "abnormal" operation, and the motor 4 will stop working for protection. Before the blade release operation is completed, that is, before the blade release button 121 is reset, the operating switch 113 in the blade slot 112 should remain in its original position even if it is in the pop-up state, to avoid the blade 306 from not sliding down completely and getting stuck. Until the blade 306 has completely slid down, the operator releases the blade eject button 121 to reset it. The motor 4 resumes operation, causing the blade slot 112 to rotate counterclockwise until it is aligned with the blade inlet 1023. The next blade 306 is then pushed in, pressing the operating switch 113. At this time, because there is a blade 306 in use, the blade eject button 121 resets, causing the third electrode contact 8c to be in a conductive state. The blade eject switch 6 is in a closed state, and the blade eject button 121 is locked and cannot be pressed. Therefore, the motor 4 will continue to operate, causing the blade slot 112 to rotate clockwise to the pen body's central axis. Because the operating switch 113 is continuously pressed, the blade slot 112 will remain at the pen body's central axis, waiting for the next blade ejection operation.

[0084] When the third electrode contact 8c changes from the on state to the off state once, the counter in the display control component 5 counts once and displays the counting result on the display screen; the count is cleared to zero after the power module 7 is turned off, and starts counting from zero after the power is turned on.

[0085] The first electrode contact 8a and the second electrode contact 8b can be metal sheets with slightly raised centers, corresponding to the contact surfaces of adjacent components. When the contact surfaces of adjacent components are misaligned, the circuit is broken; when the contact surfaces of adjacent components are in contact, the circuit is connected. The third electrode contact 8c is a ring-shaped contact electrode. The inner ring is a point electrode with the opposite polarity to the outer ring electrode. The rings are insulated from each other. At the same time, it forms a conductive circuit after contacting the raised metal top surface of the frustum at the upper end of the cutter head 306.

[0086] like Figure 18 , Figures 20-22 As shown, the tool unloading assembly 13 includes a tool unloading button 131. The button base of the tool unloading button 131 has a first tool extension slot 131a that matches the first tool extension rod 122. The first tool extension rod 122 passes through the first tool extension slot 131a and can move back and forth within it. When the tool extension button 121 is pressed, the first tool extension rod 122 reaches the end point of the first tool extension slot 131a near the tool unloading button 131, at which point the tool unloading button 131 cannot be pressed. When the tool extension button 121 returns to its initial state, the tool unloading button 131 can be pressed. When the tool unloading button 131 is pressed, the end point of the first tool extension slot 131a approaches the first tool extension rod 122, and the tool extension button 121 cannot be pressed. When the tool unloading button 131 returns to its original position, the tool extension button 121 can be pressed.

[0087] The counter-pressure and mutually exclusive structure of the cutter button 121 and the cutter unloading button 131 ensures that the cutter operation and the cutter unloading operation are mutually exclusive. That is, during the cutter operation, the cutter unloading button 131 is in a state to prevent accidental touch, and during the cutter unloading operation, the cutter button 121 is in a state to prevent accidental touch.

[0088] The unloading button 131 is located away from the first tool release bar through groove 131a. The unloading rod 132 is vertically installed at the other end. The unloading rod 132 is always perpendicular to the pressing and rebound direction of the unloading button 131. The bottom end of the unloading rod 132 is fitted with an unloading pull rod 133. The unloading pull rod 133 can move up and down relative to the rod body of the unloading rod 132.

[0089] Two sets of scissor structures are symmetrically fitted at the other end of the cutter unloader 133 away from the cutter unloader 132, and the bottom of the two sets of scissor structures are surrounded by a bottom groove 14.

[0090] Specifically, the two sets of scissor lift structures include two sets of symmetrically arranged forks 134. The two sets of forks 134 rotate in an "X" shape around two central fixed shafts 137, which are fixedly connected to the bottom shell 103. The top ends of the two forks 134 in the same set are respectively provided with a first movable shaft 135 and a second movable shaft 136. The first movable shaft 135 and the second movable shaft 136 simultaneously pass through a balance groove rod 138, which slides synchronously up and down in symmetrically arranged grooves in the bottom shell 103. The balance groove rod 138, the first movable shaft 135 and the second movable shaft 136 ensure that the apexes of the two forks 134 in the same set are always at the same horizontal plane. The bottom ends of the two sets of opposing forks 134 are respectively fixedly provided with swing shafts 139. Each swing shaft 139 has a semi-groove in the middle, which forms a bottom groove 14 when they are in contact. The balance bar 138 is perpendicular to the rocker shaft 139. The balance bar 138 ensures that the apex height of the "X"-shaped fork 134 remains consistent, while the rocker shaft 139 ensures that the bottom height of the opposite fork 134 remains consistent. When the forks 134 of the two sets of scissor lift structures rotate around the central fixed shaft 137, the two semi-grooves of the bottom groove 14 that are in contact can separate relative to each other.

[0091] The bottom of the bottom groove 14 is provided with an opening. When the bottom groove 14 is in the initial closed state, the upper truncated cone protrusion of the blade 306 that slides out from the blade slide cavity 1014 is stuck in the bottom groove 14, and the thinner blade passes through the opening and protrudes outside the bottom shell 103 of the pen body assembly 1.

[0092] The other end of the blade release lever 133, away from the blade release lever 132, is symmetrically sleeved on the inner ends of the two first movable shafts 135. When the blade release button 131 is pressed, the blade release lever 132 drives the blade release lever 133 to move away from the central axis of the pen body. The blade release lever 133 pulls the first movable shafts 135 on both sides, causing the fork 134 to rotate around the central fixed shaft 137. The height of the second movable shafts 136 on both sides is simultaneously lowered through the balance groove rod 138. That is, the two sets of scissor fork structures rotate synchronously, causing the swing shaft 139 to rise synchronously, thereby causing the bottom groove 14 to separate and open, allowing the blade 306, which was originally located in the bottom groove 14, to slide out of the pen body assembly 1.

[0093] The bottom shell 103 has two sets of symmetrical slotted structures that match the scissor lift structure, which are used to provide support and limit the two sets of scissor lift structures. In addition, the front shell 101 and the back shell 102 form a cutter bar limiting groove 1013a, so that the cutter bar 132 always moves back and forth in the same vertical direction.

[0094] In the tumor cell enrichment device with automatic counting and blade replacement provided by this invention, the blade 306 is a disposable consumable; the overall diameter and length of the pen body can be adaptively adjusted according to actual usage needs; the handle is located below the blade box 2, and its diameter is suitable for gripping. The blade box 2 can hold at least two blade boxes 3 simultaneously, and the length of the blade box 2 can be adjusted according to actual usage needs without affecting the gripping function to hold more blade boxes 3. For example, in the accompanying drawings of this invention, the blade box 2 can hold 5 blade boxes 3 simultaneously.

[0095] Obviously, the embodiments described above are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

Claims

1. A tumor cell enrichment device with automatic counting and blade changing, characterized in that, include: The pen body assembly (1) and the knife box (2) detachably mounted on the shell of the pen body assembly (1); multiple knife boxes (3) are slidably installed in the knife box (2), and the knife box (3) located at the bottom is accurately aligned with the target position on the shell of the pen body assembly (1); the knife box (3) encapsulates a number of knife heads (306) to be used. The pen body assembly (1) includes a housing assembly, which has corresponding slots and cavities for installing the operating assembly, the blade ejection assembly (12), the blade unloading assembly (13), the control assembly, and the power module (7); the housing assembly also has necessary electrical wiring pathways to ensure the circuit connectivity of the internal electronic components. The operating component is used to automatically transfer the blade (306) from the outside of the pen body assembly (1) housing to the inside; the blade dispensing component (12) is used to control the blade (306) to automatically slide down from the inside of the pen body assembly (1) to the bottom to expose the blade tip and fix it; the blade unloading component (13) is used to control the blade (306) to automatically detach from the bottom of the pen body assembly (1); the control component includes a display control component (5) for controlling, calculating and displaying the current number of blades (306) in use; the power module (7) provides power to the entire device.

2. The tumor cell enrichment device according to claim 1, characterized in that, The housing assembly includes a front shell (101), a back shell (102), a bottom shell (103), and a top cover (104); a blade inlet (1023) is provided at the bottom of the back shell (102); a running cavity (1011) is provided inside the housing corresponding to the blade inlet (1023); a blade slide cavity (1014) is provided at the lower part of the running cavity (1011); the central axis of the blade slide cavity (1014) coincides with the central axis of the pen body assembly (1); a first baffle groove (1012b) is provided between the bottom of the running cavity (1011) and the top of the blade slide cavity (1014); a second baffle groove (1012d) is provided at the bottom of the blade slide cavity (1014).

3. The tumor cell enrichment device according to claim 2, characterized in that, The upper end of the cutter head (306) is provided with a frustum-shaped protrusion. The top surface of the frustum-shaped protrusion is made of a conductive metal surface, and the rest is insulated.

4. The tumor cell enrichment device according to claim 3, characterized in that, The operating components include a shaft-connected operating module (11) and an operating motor (4). The operating module (11) is rotatably disposed in the operating cavity (1011) along its own axis. The circumferential sidewall of the operating module (11) is eccentrically provided with a cutter head groove (112). The groove diameter of the cutter head groove (112) matches the maximum cross-sectional diameter of the cutter head (306). An operating switch (113) is provided on the upper inner side of the cutter head groove (112). The operating switch (113) is electrically connected to the operating motor (4). The output end housing of the motor (4) is provided with a running limit block (42), and the rotating rod of the output end (41) is provided with a protrusion. The running limit block (42) is a semi-circular ring structure that surrounds the output end (41) and matches the protrusion. When the operating module (11) is in the initial state, the opening of the blade groove (112) is directly opposite the blade inlet (1023); when the blade (306) is pushed into the blade groove (112), it presses the switch of the operating switch (113) to retract into the side wall of the blade groove (112), and the operating motor (4) rotates clockwise, so that the blade groove (112) and the blade (306) therein rotate to the axis of the pen body assembly (1), directly opposite the top opening of the blade slide cavity (1014); when the blade (306) is disengaged from the blade groove (112), the switch of the operating switch (113) pops out again from the side wall of the blade groove (112), the operating motor (4) rotates counterclockwise, and the opening of the blade groove (112) is directly opposite the blade inlet (1023) again.

5. The tumor cell enrichment device according to claim 4, characterized in that, The blade ejection assembly (12) includes a blade ejection button (121), and the tail end of the blade ejection button (121) is vertically provided with a two-stage linkage structure; The first-stage linkage structure includes a first outgoing blade (122) and a first baffle (123); the top end of the first outgoing blade (122) is fixedly connected to the tail end of the outgoing blade button (121), and the bottom end of the first outgoing blade (122) is fixedly connected to the tail end of the first baffle (123); the first baffle (123) can slide horizontally in the first baffle groove (1012b); when the outgoing blade button (121) is in the initial state, the first baffle (123) blocks the channel between the blade head groove (112) and the blade head slide cavity (1014); when the outgoing blade button (121) is pressed, the first baffle (123) moves backward to connect the channel between the blade head groove (112) and the blade head slide cavity (1014); The second-stage linkage structure includes a second blade extension rod (124) and a second baffle (125); the top end of the second blade extension rod (124) is fixedly connected to the bottom of the first baffle (123), and the bottom end of the second blade extension rod (124) is fixedly connected to the tail end of the second baffle (125); the second baffle (125) can translate in the second baffle groove (1012d); the first blade extension rod (122) and the second blade extension rod (124) are always perpendicular to the pressing and rebound direction of the blade extension button (121); When the cutter button (121) is pressed, the second baffle (125) moves backward to allow the cutter head (306) that has fallen above to pass through; when the cutter button (121) springs back to its initial state, the bottom surface of the second baffle (125) abuts against the top surface of the frustum-shaped protrusion at the upper end of the fallen cutter head (306) to prevent the cutter head (306) in use from being pushed back.

6. The tumor cell enrichment device according to claim 5, characterized in that, The upper end of the second baffle groove (1012d) is provided with a first electrode contact (8a), the top surface of the second baffle (125) is provided with a second electrode contact (8b), and the bottom surface of the second baffle (125) is provided with a third electrode contact (8c). When the second baffle (125) is in the initial state, the first electrode contact (8a) and the second electrode contact (8b) are connected in a conductive manner; when the second baffle (125) moves backward, the first electrode contact (8a) and the second electrode contact (8b) are disconnected; when the metal top surface of the frustum-shaped protrusion at the upper end of the cutter head (306) abuts against the bottom surface of the second baffle (125), the third electrode contact (8c) is in a conductive state; when the bottom surface of the second baffle (125) is suspended, the third electrode contact (8c) is in a broken circuit state.

7. The tumor cell enrichment device according to claim 6, characterized in that, The blade ejection assembly (12) also includes a blade ejection switch (6). When the blade ejection switch (6) is closed, its on / off switch prevents the first blade ejection rod (122) from moving backward. The blade ejection switch (6) is connected to the display control assembly (5) via the first electrode contact (8a), the second electrode contact (8b), and the third electrode contact (8c). When the first electrode contact (8a) and the second electrode contact (8b) are connected, and the third electrode contact (8c) is in a conducting state, the knife-out switch (6) is in a closed state, and the knife-out button (121) is locked and cannot be pressed; when the third electrode contact (8c) is in a broken circuit state, the knife-out switch (6) is in an open state, and the knife-out button (121) can be pressed; after the knife-out button (121) is pressed, the first electrode contact (8a) and the second electrode contact (8b) are disconnected, and the knife-out switch (6) remains in an open state; When the knife-out switch (6) is in the open state, the running motor (4) stops working; when the knife-out switch (6) is in the closed state, the running motor (4) resumes working; when the third electrode contact (8c) changes from the conducting state to the disconnected state once, the calculator in the display control component (5) counts once and displays the counting result on the display screen; the power module (7) resets the count to zero after being turned off, and starts counting from zero after being turned on.

8. The tumor cell enrichment device according to claim 7, characterized in that, The tool unloading assembly (13) includes a tool unloading button (131). The button seat of the tool unloading button (131) has a first tool extension rod through groove (131a) that matches the first tool extension rod (122). The first tool extension rod (122) passes through the first tool extension rod through groove (131a) and can move back and forth in the first tool extension rod through groove (131a). The unloading button (131) has an unloading rod (132) vertically mounted at the other end away from the first tool release bar groove (131a). The unloading rod (132) is always perpendicular to the pressing and rebound direction of the unloading button (131). The bottom end of the unloading rod (132) is fitted with an unloading pull rod (133). The other end of the unloading pull rod (133) away from the unloading rod (132) is symmetrically fitted with two sets of scissor structures. The bottom ends of the two sets of scissor structures are surrounded by a bottom groove (14). When the unloading button (131) is pressed, the unloading rod (132) drives the unloading pull rod (133) to move outward. The unloading pull rod (133) pulls the two sets of scissor structures to rotate synchronously, causing the bottom groove (14) to separate and open, thereby causing the blade (306) originally located in the bottom groove (14) to slide out of the housing of the pen body assembly (1).

9. The tumor cell enrichment device according to claim 8, characterized in that, The two sets of scissor lift structures include two sets of symmetrically arranged forks (134). The two sets of forks (134) rotate in an "X" shape around two central fixed axes (137). The two central fixed axes (137) are fixedly connected to the bottom shell (103). The top ends of the two forks (134) in the same set are respectively provided with a first movable shaft (135) and a second movable shaft (136). The first movable shaft (135) and the second movable shaft (136) are simultaneously provided with a balance groove rod (138). The bottom ends of the two sets of opposing forks (134) are respectively fixedly provided with a rocker shaft (139). Each of the two rocker shafts (139) is provided with a semi-groove in the middle. When the two semi-grooves are in contact, they surround and form the bottom groove (14).

10. The tumor cell enrichment device according to claim 1, characterized in that, The knife box (3) includes a knife box shell (301), inside which a knife box spring (305) and a push plate (303) are provided; the knife box spring (305) pushes the push plate (303) to slide inside the knife box shell (301) and push out the knife head (306); a knife box limiting block (304) is provided in the middle of the side of the push plate (303), and the knife box limiting block (304) slides out of the outside of the knife box shell (301); a knife box limiting groove (202) is provided on the box plate of the knife box (2), and the knife box limiting block (304) can slide in the knife box limiting groove (202).

Images