Micro-fluidic biochip, gas circuit board, micro-fluidic biological analyzer and micro-fluidic biochip application assembly

Abstract

The utility model discloses a micro-fluidic biochip, a gas circuit board, a micro-fluidic biological analyzer and a micro-fluidic biochip application assembly, and relates to the technical field of micro-fluidic chips, the micro-fluidic biochip comprises a substrate, a silica gel pad and a cover plate; a switching valve, a membrane pump and a flow channel are arranged on the substrate; the silica gel pad is positioned between the substrate and the cover plate; air holes are formed in the positions, corresponding to the switching valve and the membrane pump, of the cover plate and used for being communicated with the air holes in the air path plate. Corresponding connecting areas are arranged on the cover plate, the silica gel pad and the substrate and used for tightly connecting the substrate and the cover plate and pressing the silica gel pad between the substrate and the cover plate. By adopting the scheme, the complexity and difficulty of the preparation process of the microfluidic biochip are reduced, and the microfluidic biochip is more convenient to use.

Description

Technical Field

[0001] The present application relates to the technical field of microfluidic chips, and in particular to a microfluidic biochip, an air path plate and a microfluidic bioanalyzer. Background Art

[0002] Microfluidics chip technology integrates the basic operation units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis process into a micrometer-scale chip to automatically complete the entire analysis process. Microfluidics chips are currently a hot area in the development of micro-total analysis systems.

[0003] Microfluidic chip analysis uses the chip as the operating platform, is based on analytical chemistry, relies on micro-electromechanical processing technology, has a micro-channel network as the structural feature, and is currently mainly applied to life sciences. It is the focus of the current development of the micro-total analysis system. Its goal is to integrate the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc., on a microchip, and it can be used multiple times. In the process of producing microfluidic chips, it is necessary to pre-embed a variety of reagents in the chip, such as lysate, washing solution, eluent, etc.

[0004] At present, in the production process of microfluidic chips, the composite membrane needs to be bonded to the holes and flow channel surfaces of the substrate (also called chip) by bonding methods (such as laser welding, ultrasonic welding, hot pressing, etc.) so that the flow channel forms a closed flow channel, switch valve and membrane pump. Its structure is as follows Figure 1 and Figure 2 As shown. The flow channel is used for the circulation of gas and liquid, and the switch valve and membrane pump are used to control the circulation of gas or liquid. During the use of the microfluidic chip, the microfluidic chip is placed on the gas path plate of the microfluidic bioanalyzer, and the upper surface of the gas path plate is distributed with air holes corresponding to the positions of the switch valve and membrane pump of the microfluidic chip. According to the use requirements, the air holes of the gas path plate can output positive pressure air and negative pressure air to complete the compression and absorption of the composite membrane at the bottom of the microfluidic chip, so that the switch valve and membrane pump are formed between the composite membrane and the substrate, thereby completing the function of pushing the gas or liquid to flow along the flow channel.

[0005] like Figure 3 As shown, during the use of the microfluidic chip and the gas circuit board, in order to seal between the gas circuit board and the microfluidic chip, a layer of silicone pad needs to be laid on the upper surface of the gas circuit board. Double-sided tape can be used to bond the silicone pad and the gas circuit board, and holes are opened on the silicone pad at the positions corresponding to the switch valve and membrane pump of the microfluidic chip to communicate with the air holes on the gas circuit board.

[0006] At present, the microfluidic chips and gas circuit boards produced by this process have some obvious defects and deficiencies. For the bonding process of the substrate and the composite membrane, if laser welding is used, the welding equipment is expensive, most of which needs to be imported, and the maintenance and care are complicated. If the hot pressing process is used, the substrate is heated and deformed seriously, which often causes distortion and blockage of the substrate flow channel. The heat also has a certain effect on the embedded reagents. In addition, the gas circuit board needs to be customized with silicone pads. When the silicone pads are laid on the gas circuit board, they are bonded by double-sided tape. After long-term use, the double-sided tape is easy to fail and the silicone pad is easy to deform. Summary of the invention

[0007] The embodiments of the present application provide a microfluidic biochip, an air path plate and a microfluidic bioanalyzer to solve the problems in the prior art that the preparation process of the microfluidic biochip is complicated and the use is inconvenient.

[0008] The embodiment of the present application provides a microfluidic biochip, comprising: a substrate, a silicone pad and a cover sheet;

[0009] The substrate is provided with a switch valve, a membrane pump and a flow channel;

[0010] The silica gel pad is located between the base sheet and the cover sheet;

[0011] The cover sheet is provided with air holes at positions corresponding to the switch valve and the membrane pump, which are used to communicate with the air holes on the gas path plate;

[0012] The cover sheet, the silicone pad and the base sheet are provided with corresponding connection areas for tightly connecting the base sheet and the cover sheet and for pressing the silicone pad between the base sheet and the cover sheet.

[0013] Furthermore, the silicone pad has protrusions at positions corresponding to the edges of the switch valve, the membrane pump and the flow channel.

[0014] Furthermore, the edges of the switch valve, the membrane pump and the flow channel on the substrate have protrusions.

[0015] Furthermore, the cover sheet, the silicone pad and the substrate are provided with a plurality of corresponding connection areas;

[0016] In the connection area, the cover sheet and the base sheet are connected by a heat riveting process.

[0017] Furthermore, the cover sheet, the silicone pad and the substrate are provided with a plurality of corresponding connection areas;

[0018] In the connection area, the cover sheet and the base sheet are connected by ultrasonic welding.

[0019] Furthermore, the air holes provided on the cover plate are used for the boss air holes on the gas path plate to penetrate into the air holes, so that the boss air holes are in close contact with the silicone pad.

[0020] An embodiment of the present application also provides an air path plate, wherein a side of the air path plate in contact with the microfluidic biochip has a boss pore, wherein the boss pore is used to penetrate into the pore provided on the cover plate of the microfluidic biochip and to be in close contact with the silicone pad in the microfluidic biochip.

[0021] The present application also provides a microfluidic bioanalyzer, comprising:

[0022] The above-mentioned gas circuit plate with boss air holes.

[0023] The present application also provides a microfluidic biochip application component, including:

[0024] Any of the above-mentioned microfluidic biochips, and an air path plate with boss air holes.

[0025] The beneficial effects of this application include:

[0026] The microfluidic biochip provided in the embodiment of the present application comprises a substrate, a silicone pad and a cover sheet, wherein:

[0027] The substrate is provided with a switch valve, a membrane pump and a flow channel, the silicone pad is located between the substrate and the cover sheet, and the cover sheet is provided with air holes at positions corresponding to the switch valve and the membrane pump, which are used to communicate with the air holes on the gas path plate, and the cover sheet and the substrate are provided with corresponding connection areas, which are used to tightly connect the substrate and the cover sheet, and to compress the silicone pad between the substrate and the cover sheet. In the microfluidic biochip, the substrate and the silicone pad are in a compressed state, so that the switch valve, the membrane pump and the flow channel can be formed. Compared with the prior art, the composite membrane is no longer required, that is, the composite membrane is avoided from being bonded to the substrate by bonding. It is only necessary to tightly connect the cover sheet with the substrate and compress the silicone pad, which reduces the complexity and difficulty of the preparation process. In addition, the substrate, the silicone pad and the cover sheet are in a compressed state, so that in the process of using the microfluidic biochip, it can be directly placed on the gas path plate, and it is no longer necessary to place a silicone pad between the gas path plate, so that it is more convenient to use.

[0028] Other features and advantages of the present application will be described in the following description, and partly become apparent from the description, or be understood by practicing the present application. The purpose and other advantages of the present application can be realized and obtained by the structures specifically pointed out in the written description, claims, and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The accompanying drawings are used to provide a further understanding of the present application and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the present application and do not constitute a limitation of the present application. In the accompanying drawings:

[0030] Figure 1 It is a schematic diagram of a cross-sectional structure of a microfluidic biochip in the prior art from a top view;

[0031] Figure 2 It is a schematic diagram of the front structure of a microfluidic biochip in the prior art;

[0032] Figure 3 It is a schematic diagram of the structure of a microfluidic biochip placed on a gas path plate when in use in the prior art;

[0033] Figure 4 This is a schematic diagram of the top view of the microfluidic biochip in the embodiment of the present application;

[0034] Figure 5 This is a schematic structural diagram of a cross section at position C on the front side of a microfluidic biochip in an embodiment of the present application;

[0035] Figure 6 Schematic diagram of the cross-sectional structure of a microfluidic biochip using a heat riveting process in an embodiment of the present application;

[0036] Figure 7 This is a schematic structural diagram of a cross section at position A on the front side of a microfluidic biochip using a heat riveting process in an embodiment of the present application;

[0037] Figure 8 This is a schematic diagram of the structure of the microfluidic biochip placed on the gas path plate when in use in an embodiment of the present application. DETAILED DESCRIPTION

[0038] In order to provide a solution to reduce the complexity and difficulty of the preparation process of the microfluidic biochip and make it more convenient to use, the embodiments of the present application provide a microfluidic biochip, a gas path plate and a microfluidic bioanalyzer. The preferred embodiments of the present application are described below in conjunction with the drawings of the specification. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present application and are not used to limit the present application. In addition, the embodiments in the present application and the features in the embodiments can be combined with each other if there is no conflict.

[0039] The present application provides a microfluidic biochip, such as Figure 4 and Figure 5 As shown, it includes: a substrate, a silicone pad and a cover sheet;

[0040] A switch valve, a membrane pump and a flow channel are arranged on the substrate;

[0041] The silicone pad is located between the base sheet and the cover sheet;

[0042] The cover sheet is provided with air holes at positions corresponding to the switch valve and the membrane pump, which are used to communicate with the air holes on the gas path plate;

[0043] The cover sheet, the silicone pad and the base sheet are provided with corresponding connection areas for tightly connecting the base sheet and the cover sheet and for pressing the silicone pad between the base sheet and the cover sheet.

[0044] In the above-mentioned microfluidic biochip provided in the embodiment of the present application, the substrate and the silicone pad are in a compressed state, so that a switch valve, a membrane pump and a flow channel can be formed. Compared with the prior art, a composite membrane is no longer required, that is, it is avoided to bond the composite membrane to the substrate by bonding. It is only necessary to tightly connect the cover sheet and the substrate and press the silicone pad, thereby reducing the complexity and difficulty of the preparation process. Moreover, the substrate, the silicone pad and the cover sheet are in a compressed state, so that in the process of using the microfluidic biochip, it can be directly placed on the air path plate, and there is no need to place a silicone pad between the air path plate, which is more convenient to use.

[0045] Compared with the existing technology, the composite membrane and the silicone pad placed on the gas path plate are reduced. Although a cover sheet and a silicone pad are added to the microfluidic chip, overall, the types of materials are reduced and the overall cost is reduced.

[0046] In the embodiment of the present application, the cover sheet and the base sheet can be directly and tightly connected, for example, Figure 6 , Figure 7 As shown, the cover sheet, the silicone pad and the substrate are provided with a plurality of corresponding connection areas, and in the connection areas, the cover sheet and the substrate can be connected by a hot riveting process;

[0047] Specifically, multiple areas for inserting hot rivet pins can be set on the cover sheet and the silicone pad as connection areas, and the hot rivet pins are used to pass through the cover sheet and the silicone pad and act on the substrate. After hot riveting, the cover sheet and the substrate are tightly connected, and the silicone pad between the substrate and the cover sheet is compressed, and the corresponding flow channel, switch valve and membrane pump can be formed between the silicone pad and the substrate.

[0048] In another embodiment of the present application, a plurality of corresponding connection areas may be provided on the cover sheet, the silicone pad and the substrate. Specifically, in the connection areas, the cover sheet and the substrate may be connected by ultrasonic welding technology.

[0049] In the embodiment of the present application, the silicone pad is pressed against the substrate to form a switch valve, a membrane pump and a flow channel. In order to improve the sealing effect of the switch valve, membrane pump and flow channel, a protrusion may be provided on the silicone pad at a position corresponding to the edge of the switch valve, membrane pump and flow channel, or a protrusion may be provided at the edge of the switch valve, membrane pump and flow channel on the substrate. When the silicone pad is pressed against the substrate, the edges of the switch valve, membrane pump and flow channel can be pressed more tightly because of the protrusion, so the sealing effect is better.

[0050] During the use of the microfluidic biochip provided in the embodiments of the present application, Figure 8 As shown, the microfluidic biochip can be placed above the air circuit board of the microfluidic bioanalyzer, wherein one side of the cover sheet of the microfluidic biochip is in contact with the air circuit board, and the cover sheet is provided with air holes which communicate with the air holes on the air circuit board. The microfluidic bioanalyzer can apply negative pressure air or positive pressure air to the microfluidic biochip through the air circuit board. When negative pressure is applied, the silicone pad moves downward to form a cavity between the silicone pad and the substrate, through which liquid can flow, which is equivalent to opening the valve. When positive pressure is applied, the silicone pad fits and is compacted with the substrate, which can cut off the flow of body fluids, which is equivalent to closing the valve. When negative pressure and positive pressure act alternately and continuously, the liquid can be continuously pushed forward and circulate in the flow channel. At this time, when negative pressure of the membrane pump is formed, it is equivalent to creating space, and when positive pressure of the membrane pump is formed, there is no space.

[0051] In the embodiment of the present application, it is no longer necessary to place a silicone pad between the microfluidic biochip and the air circuit plate. In order to generate better negative pressure and positive pressure between the air circuit plate and the silicone pad of the microfluidic biochip, the air holes communicating between the cover plate and the air circuit plate can be more closely connected. Furthermore, the air holes on the air circuit plate can be set as boss air holes, that is, the air holes on the air circuit plate are convex in shape and can penetrate into the air holes set on the cover plate, so that the boss air holes are in close contact with the silicone pad, thereby reducing gas leakage in the process of generating negative pressure and positive pressure, and obtaining better negative pressure and positive pressure effects.

[0052] Based on a microfluidic biochip provided in an embodiment of the present application, a corresponding embodiment of the present application also provides an air path plate, on which a side in contact with the microfluidic biochip has boss holes, and the boss holes are used to penetrate into the holes set on the cover plate of the microfluidic biochip and to be in close contact with the silicone pad in the microfluidic biochip.

[0053] Accordingly, the present application also provides a microfluidic bioanalyzer, comprising:

[0054] The above-mentioned gas circuit plate with boss air holes.

[0055] Accordingly, the present application also provides a microfluidic biochip application component, including:

[0056] Any of the above-mentioned microfluidic biochips, and an air path plate with boss air holes.

[0057] In summary, the microfluidic biochip provided in the embodiment of the present application uses a silicone pad instead of a composite membrane, and a flow channel, a switch valve, and a membrane pump are formed between the substrate and the silicone pad; at the same time, the silicone pad on the upper layer of the gas path plate is omitted. A layer of silicone pad on the bottom surface of the substrate replaces the original composite membrane and the original gas path plate silicone pad, which reduces the types of materials, reduces the complexity and difficulty of the process, eliminates the bonding process, reduces the overall cost, and is more convenient to use.

[0058] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0059] Obviously, those skilled in the art can make various changes and modifications to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the present application is also intended to include these modifications and variations.

Claims

1. A microfluidic biochip, characterized in that: include: Substrate, silicone pad and cover sheet; The substrate is provided with a switch valve, a membrane pump and a flow channel; The silica gel pad is located between the base sheet and the cover sheet; The cover sheet is provided with air holes at positions corresponding to the switch valve and the membrane pump, which are used to communicate with the air holes on the gas path plate; The cover sheet, the silicone pad and the base sheet are provided with corresponding connection areas for tightly connecting the base sheet and the cover sheet and for pressing the silicone pad between the base sheet and the cover sheet.

2. The microfluidic biochip according to claim 1, characterized in that: The silicone pad has protrusions at positions corresponding to the switch valve, the membrane pump and the edges of the flow channel.

3. The microfluidic biochip according to claim 1, characterized in that: The edge positions of the switch valve, the membrane pump and the flow channel on the substrate have protrusions.

4. The microfluidic biochip according to claim 1, characterized in that: The cover sheet, the silicone pad and the base sheet are provided with a plurality of corresponding connection areas; In the connection area, the cover sheet and the base sheet are connected by a heat riveting process.

5. The microfluidic biochip according to claim 1, characterized in that: The cover sheet, the silicone pad and the base sheet are provided with a plurality of corresponding connection areas; In the connection area, the cover sheet and the base sheet are connected by ultrasonic welding.

6. The microfluidic biochip according to claim 1, characterized in that: The air holes arranged on the cover plate are used for the boss air holes on the air path plate to penetrate into the air holes, so that the boss air holes are in close contact with the silicone pad.

7. A gas path plate, characterized in that: The side of the gas path plate in contact with the microfluidic biochip has a boss pore, and the boss pore is used to penetrate into the pores provided on the cover sheet of the microfluidic biochip and closely contact with the silicone pad in the microfluidic biochip.

8. A microfluidic bioanalyzer, characterized in that: include: The gas circuit board as claimed in claim 7.

9. A microfluidic biochip application component, characterized in that: include: A microfluidic biochip as described in any one of claims 1 to 6, and a gas path plate as described in claim 7.

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