Extraction and purification instrument based on superparamagnetic beads

Abstract

The utility model belongs to the technical field of antibody and nucleic acid extraction and purification, and discloses an extraction and purification instrument based on superparamagnetic beads, which comprises a box body, one side of the box body is rotatably connected with a box door, an extraction base and a purification base are respectively arranged on the inner bottom surface of the box body, a plurality of desalination columns are arranged on the purification base, and purification components are arranged in the desalination columns. The motor is fixedly installed on the inner side wall of the box body, an output shaft of the motor is fixedly connected with a lead screw, the lead screw is in transmission connection with a sliding block, a first air cylinder and a second air cylinder are fixedly installed on the bottom face of the sliding block, a magnetic bar assembly is arranged on an output shaft of the first air cylinder, and a sampling assembly is arranged on an output shaft of the second air cylinder. According to the utility model, the integrated extraction and purification of antibodies and nucleic acids are realized, the quality and flux of samples are improved, the property parameters of the obtained nucleic acids and antibodies are more stable, the automatic operation is realized, the manual intervention and errors are reduced, and the consistency and stability of the samples are enhanced.

Description

Technical Field

[0001] The utility model belongs to the technical field of antibody and nucleic acid extraction and purification, and particularly relates to an extraction and purification instrument based on superparamagnetic beads. Background Art

[0002] At present, the commonly used nucleic acid extraction and purification methods in the laboratory include organic solvent method, silica gel membrane method, etc.; antibody purification methods include dialysis based on different antibody molecular sizes and water solubility, affinity chromatography column method based on different ligands, and high performance liquid chromatography based on different mobile phase distribution coefficients. However, the above methods are difficult to automate, and there are problems such as difficulty in scaling up from laboratory small-scale test systems to actual production processes, and difficulty in automated operation, resulting in poor reproducibility of the obtained nucleic acid and antibody property parameters.

[0003] Superparamagnetic beads are micron-sized magnetic particles with specific ligands on their surface. They can bind specifically to target molecules and then be adsorbed and released using an external magnetic field to separate and enrich the target molecules. Due to the modifiability of the ligands contained in the magnetic beads, they are widely used in the extraction and purification of biological molecules (nucleic acids, antibodies). In addition, superparamagnetic beads are simple to operate, highly sensitive, highly selective, and suitable for automation, making them an ideal sample preparation material.

[0004] Therefore, there is an urgent need for an extraction and purification instrument based on superparamagnetic beads for automatic extraction and purification of nucleic acids and antibodies. Utility Model Content

[0005] In order to solve the above technical problems, the utility model proposes an extraction and purification instrument based on superparamagnetic beads, aiming to solve or improve at least one of the above technical problems.

[0006] To achieve the above object, the utility model provides an extraction and purification instrument based on superparamagnetic beads, comprising:

[0007] A box body, one side of which is rotatably connected with a box door, an extraction base and a purification base are respectively arranged on the bottom surface of the box body, a sample pool, a magnetic bead pool, a first rinsing pool, a second rinsing pool, a cleaning pool and an elution pool are arranged on the extraction base, a plurality of desalting columns are arranged on the purification base, the desalting columns are connected with a liquid inlet head, a purification component is arranged in the desalting column, a piston is arranged above the purification component, a collection tube is correspondingly arranged below the desalting column, and a driving component corresponding to the upper and lower parts of the piston is arranged above the desalting column;

[0008] A motor, wherein the motor is fixedly mounted on the inner wall of the box body, a lead screw is fixedly connected to the output shaft of the motor, a slider is transmission-connected to the lead screw, a pair of optical rods slide through the slider, the optical rods are fixedly mounted on the side wall of the box body, a first cylinder and a second cylinder are fixedly mounted on the bottom surface of the slider, a magnetic rod assembly is provided on the output shaft of the first cylinder, and a sampling assembly is provided on the output shaft of the second cylinder, the first cylinder and the second cylinder are driven by the sliding of the slider, the magnetic rod assembly can slide along the extraction base, and the sampling assembly can correspond to the liquid inlet head up and down.

[0009] Optionally, the extraction base includes a base frame, a ninety-six-well plate is placed on the base frame, and the sample pool, the magnetic bead pool, the first rinsing pool, the second rinsing pool, the cleaning pool and the elution pool are arranged on the ninety-six-well plate.

[0010] Optionally, the purification base includes a first support plate, the first support plate is fixedly connected to the inner wall of the box body, a row of first through holes is formed on the first support plate, and the desalting columns are inserted into the first through holes.

[0011] Optionally, one side of the first support plate is rotatably connected to a connecting plate, a row of ring frames are fixedly connected to the connecting plate, the collecting tubes are plugged into the ring frames, one end of the connecting plate is fixedly connected to an anode magnet, the anode magnet attracts a cathode magnet, and the cathode magnet is fixedly connected to the first support plate.

[0012] Optionally, the driving assembly includes a second support plate, the second support plate is fixedly connected to the inner wall of the box body, a third cylinder is fixedly mounted on the second support plate, and the output end of the third cylinder faces the piston and is fixedly connected to a pressure plate.

[0013] Optionally, the piston includes a piston rod and a piston plate fixedly connected to each other, the piston rod extending upward from the desalting column to correspond to the pressure plate, the piston plate slidingly fits in the desalting column, and a purification chamber is provided below the piston plate, a one-way connecting component is arranged on the piston plate, and the one-way connecting component is respectively connected to the liquid inlet head and the purification chamber.

[0014] Optionally, the magnetic bar assembly includes a first mounting plate, the top surface of the first mounting plate is fixedly connected to the first cylinder output shaft, and a row of magnetic bars is fixedly mounted on the bottom surface of the first mounting plate.

[0015] Optionally, the sampling assembly includes a second mounting plate, the top surface of the second mounting plate is fixedly connected to the second cylinder output shaft, a row of sampling needles are fixedly installed on the bottom surface of the second mounting plate, the sampling needles are connected to a second air pump, and the second air pump is fixedly installed on the second mounting plate.

[0016] Compared with the prior art, the utility model has the following advantages and technical effects:

[0017] The motor drives the lead screw so that the slider drives the first cylinder and the second cylinder to slide, and the magnetic rod assembly and the sampling assembly can be driven to slide synchronously, and the magnetic rod assembly is driven to rise and fall by the first cylinder, and the solution sample is driven by the magnetic rod assembly to be transferred step by step along the sample pool, the magnetic bead pool, the first rinsing pool, the second rinsing pool, the cleaning pool and the elution pool to automatically extract the sample, and finally a high-purity target molecule solution is obtained in the elution pool, and the second cylinder drives the sampling assembly to transfer the high-purity target molecule solution in the elution pool to the purification assembly in the desalting column for purification, and then the driving assembly pushes the piston to press the target molecule solution out of the bottom of the desalting column, and collects it in the collection tube to complete the purification and collection of the target molecule, thereby realizing the integrated extraction and purification of antibodies and nucleic acids, improving the quality and throughput of samples, making the property parameters of the obtained nucleic acids and antibodies more stable, realizing automated operation, reducing manual intervention and errors, and enhancing the consistency and stability of samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 This is a schematic diagram of the internal structure of the box of the utility model;

[0021] Figure 3 for Figure 2 A partial enlarged view of middle A;

[0022] Figure 4 It is a cross-sectional view of the desalting column of the utility model;

[0023] Figure 5 for Figure 4 A partial enlarged view of B in the middle;

[0024] Figure 6 It is a three-dimensional diagram of the desalting column of the utility model;

[0025] Figure 7 It is a schematic diagram of the structure of the purification component of the utility model.

[0026] In the figure: 1, box body; 2, box door; 3, desalting column; 4, collecting tube; 5, motor; 6, screw rod; 7, slider; 8, polished rod; 9, first cylinder; 10, second cylinder; 11, bottom frame; 12, ninety-six-hole plate; 13, first support plate; 14, first through hole; 15, connecting plate; 16, ring frame; 17, anode magnet; 18, cathode magnet; 19, second support plate; 20, third cylinder; 21, pressing plate; 22, liquid inlet head; 23, piston rod; 24, piston plate; 25, purification chamber; 26, first installation Plate; 27, magnetic rod; 28, second mounting plate; 29, sampling needle; 30, second air pump; 31, second through hole; 32, liquid inlet pipe; 33, first fixed plate; 34, second fixed plate; 35, third through hole; 36, fourth through hole; 37, guide rod; 38, blocking plate; 39, spring; 40, annular baffle; 41, hinged screw; 42, threaded hole; 43, locking bolt; 44, annular top plate; 45, support column; 46, filter head; 47, annular bottom plate; 48, spring telescopic rod; 49, annular abutment plate. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0028] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0029] Reference Figure 1-Figure 7 As shown, this embodiment provides an extraction and purification instrument based on superparamagnetic beads, comprising:

[0030] A box body 1, one side of the box body 1 is rotatably connected with a box door 2, an extraction base and a purification base are respectively arranged on the bottom surface of the box body 1, a sample pool, a magnetic bead pool, a first rinsing pool, a second rinsing pool, a cleaning pool and an elution pool are arranged on the extraction base, a plurality of desalting columns 3 are arranged on the purification base, the desalting columns are connected with a liquid inlet head 22, a purification component is arranged in the desalting column, a piston is arranged above the purification component, a collection tube 4 is arranged correspondingly below the desalting column 3, and a driving component corresponding to the upper and lower parts of the piston is arranged above the desalting column 3;

[0031] Motor 5 is fixedly mounted on the inner wall of the box body 1, the output shaft of motor 5 is fixedly connected with a screw rod 6, the screw rod 6 is transmission-connected with a slider 7, a pair of light rods 8 are slidably penetrated on the slider 7, the light rods 8 are fixedly mounted on the side wall of the box body 1, a first cylinder 9 and a second cylinder 10 are fixedly mounted on the bottom of the slider 7, the output shaft of the first cylinder 9 is provided with a magnetic rod assembly, the output shaft of the second cylinder 10 is provided with a sampling assembly, the first cylinder 9 and the second cylinder 10 are driven by the sliding of the slider 7, the magnetic rod assembly can slide along the extraction base, and the sampling assembly can correspond to the liquid inlet head 22 up and down.

[0032] The motor 5 drives the screw rod 6 so that the slider 7 drives the first cylinder 9 and the second cylinder 10 to slide, which can synchronously drive the magnetic rod assembly and the sampling assembly to slide, and the first cylinder 9 drives the magnetic rod assembly to rise and fall, and the magnetic rod assembly drives the solution sample to be transferred step by step along the sample pool, the magnetic bead pool, the first rinsing pool, the second rinsing pool, the cleaning pool and the elution pool to automatically extract the sample, and finally obtain a high-purity target molecule solution in the elution pool, and the second cylinder 10 drives the sampling assembly to transfer the high-purity target molecule solution in the elution pool to the purification assembly in the desalting column 3 for purification, and then the driving assembly pushes the piston to press the target molecule solution out of the bottom of the desalting column 3, and collects it in the collection tube 4 to complete the purification and collection of the target molecule, thereby realizing the integrated extraction and purification of antibodies and nucleic acids, improving the quality and throughput of samples, making the property parameters of the obtained nucleic acids and antibodies more stable, realizing automated operation, reducing manual intervention and errors, and enhancing the consistency and stability of samples.

[0033] According to a further optimized solution, the extraction base includes a base frame 11 , on which a ninety-six-well plate 12 is placed, and a sample pool, a magnetic bead pool, a first rinsing pool, a second rinsing pool, a cleaning pool and an elution pool are arranged on the ninety-six-well plate 12 .

[0034] According to a further optimized solution, the purification base includes a first support plate 13 , which is fixedly connected to the inner wall of the box body 1 , and a row of first through holes 14 are formed on the first support plate 13 , and the desalting columns 3 are inserted into the first through holes 14 .

[0035] The desalting column 3 is inserted into the first through hole 14 and the outer ring of the top of the desalting column 3 is overlapped on the top of the first through hole 14 , and the desalting column 3 can be easily disassembled and replaced by opening the box door 2 .

[0036] A further optimized solution is that a connecting plate 15 is rotatably connected to one side of the first support plate 13, a row of ring frames 16 are fixedly connected to the connecting plate 15, a collecting tube 4 is inserted into the ring frames 16, an anode magnet 17 is fixedly connected to one end of the connecting plate 15, the anode magnet 17 attracts a cathode magnet 18, and the cathode magnet 18 is fixedly connected to the first support plate 13.

[0037] By overlapping the outer ring of the top opening of the collecting tube 4 with the ring frame 16, the collecting tube 4 can be easily replaced. When the collecting tube 4 is inserted into the ring frame 16, the connecting plate 15 is rotated to make the anode magnet 17 and the cathode magnet 18 attract each other, so that the collecting tube 4 can be directly opposite to the bottom opening of the desalting column 3, so that the collecting tube 4 and the desalting column 3 can be connected.

[0038] According to a further optimized solution, the driving assembly includes a second support plate 19 which is fixedly connected to the inner wall of the box body 1 , and a third cylinder 20 is fixedly mounted on the second support plate 19 . The output end of the third cylinder 20 faces the piston and is fixedly connected to a pressure plate 21 .

[0039] A further optimized solution is that the piston includes a piston rod 23 and a piston plate 24 which are fixedly connected to each other. The piston rod 23 extends upward from the desalting column 3 and corresponds to the pressure plate 21. The piston plate 24 is slidably fitted in the desalting column 3, and a purification chamber 25 is provided below the piston plate 24. A one-way connecting component is provided on the piston plate 24, and the one-way connecting component is connected to the liquid inlet head 22 and the purification chamber 25 respectively.

[0040] The one-way communication component includes a second through hole 31 formed on the piston plate 24, the second through hole 31 is connected to the purification chamber 25, a liquid inlet pipe 32 is fixedly connected to and connected to the second through hole 31, one end of the liquid inlet pipe 32 away from the second through hole 31 extends out of the desalting column 3 and is fixedly connected to and connected to the liquid inlet head 22, a first fixed plate 33 and a second fixed plate 34 corresponding to each other are fixedly connected in the second through hole 31, a plurality of third through holes 35 are formed on the first fixed plate 33, a plurality of fourth through holes 36 are formed on the second fixed plate 34, and a guide rod 37 is fixedly connected between the first fixed plate 33 and the second fixed plate 34, and a guide rod 37 is slidably connected to the guide rod 37. The movable sleeve is provided with a blocking plate 38, and a spring 39 is fixedly connected between the blocking plate 38 and the second fixed plate 34. There is a gap between the blocking plate 38 and the inner wall of the second through hole 31. The size of the blocking plate 38 is larger than the range formed by the multiple third through holes 35. Through the elastic force of the spring 39, the blocking plate 38 can be fitted with the first fixed plate 33 to block the multiple third through holes 35. When the liquid inlet head 22 and the liquid inlet pipe 32 are filled with liquid, the spring 39 can be compressed, and the blocking plate 38 is separated from the first fixed plate 33, so that the solution flows into the purification chamber 25 through the multiple third through holes 35 and the multiple fourth through holes 36 and enters the purification component.

[0041] In order to facilitate the replacement of the purification components in the desalting column, a mounting groove is provided on the inner wall at the bottom of the desalting column 3, an opening is provided on the side wall at the bottom of the desalting column 3, an annular baffle 40 is provided in the opening, a hinge screw 41 is provided on the annular baffle 40, the hinge screw 41 is hinged to the opening of the desalting column 3, a threaded hole 42 is provided on the outer wall, a locking bolt 43 is threadedly connected to the desalting column 3, and the locking bolt 43 is detachably connected to the threaded hole 42, and the purification component includes an annular top plate 44, which is inserted into the mounting groove, and a plurality of support columns 45 are fixedly connected to the bottom end of the annular top plate 44 at equal intervals along the circumferential direction, a filter head 46 is placed in the space surrounded by the plurality of support columns 45, and a plurality of support columns 45 are inserted with an annular bottom plate 47 (preferably The method is to open multiple grooves on the annular bottom plate 47, and set elastic extrusion parts in the grooves, so that the support column 45 is plugged into the annular bottom plate 47 through friction force), and the two ends of the filter head 46 are respectively in contact with the annular top plate 44 and the annular bottom plate 47, and the end of the annular bottom plate 47 away from the support column 45 is fixedly connected with multiple spring telescopic rods 48, and the end of the multiple spring telescopic rods 48 away from the annular bottom plate 47 is fixedly connected with an annular abutment plate 49, and the outer wall of the annular abutment plate 49 is a circumferentially inclined surface, and the inclined surface of the outer wall of the annular abutment plate 49 contacts the annular baffle 40, so that the annular abutment plate 49 is squeezed, and the spring telescopic rod 48 is compressed, so that the annular top plate 44 can fit tightly with the installation groove and is fixed by the locking bolt 43.

[0042] According to a further optimized solution, the magnetic rod assembly includes a first mounting plate 26 , the top surface of the first mounting plate 26 is fixedly connected to the output shaft of the first cylinder 9 , and a row of magnetic rods 27 is fixedly mounted on the bottom surface of the first mounting plate 26 .

[0043] The first cylinder 9 drives the first mounting plate 26 and the magnetic rods 27 to move. The number of the magnetic rods 27 is the same as the number of holes in each column of the ninety-six-hole plate 12, and they correspond one to one.

[0044] A further optimized solution is that the sampling assembly includes a second mounting plate 28 , the top surface of the second mounting plate 28 is fixedly connected to the output shaft of the second cylinder 10 , a row of sampling needles 29 are fixedly installed on the bottom surface of the second mounting plate 28 , the sampling needles 29 are connected to a second air pump 30 , and the second air pump 30 is fixedly installed on the second mounting plate 28 .

[0045] The second cylinder 10 drives the second mounting plate 28 and the sampling needle 29 to move, and forms positive and negative pressures through the second air pump 30, so that the sampling needle 29 can collect the solution. The number of sampling needles 29 is the same as the number of holes in each column of the ninety-six-hole plate 12, and they correspond one to one.

[0046] Working principle:

[0047] First, the sample to be extracted (such as blood, cells, tissues, etc.) is added to a sample pool containing a specific lysis solution for incubation and lysis;

[0048] Then, the slider 7 drives the magnetic rod 27 to move horizontally to the top of the magnetic bead pool, and the first cylinder 9 drives the magnetic rod 27 to descend into the magnetic bead pool. The magnetic rod 27 generates magnetic force to adsorb the magnetic beads to the magnetic rod 27. Then, the first cylinder 9 drives the magnetic rod 27 to rise, and the slider 7 drives the magnetic rod 27 to move horizontally to the top of the sample pool. The first cylinder 9 drives the magnetic rod 27 to descend into the sample pool, and the magnetic switch of the magnetic rod 27 is turned off. Then, the magnetic rod 27 moves up and down repeatedly to make the magnetic beads fully mixed with the sample for extraction. Then, the magnetic rod 27 turns on the magnetic switch to adsorb the target molecules in the lysed sample; then, the first cylinder 9 drives the magnetic rod 27 to descend into the rinsing pool, and the magnetic bead-target molecule complex is transferred from the magnetic bead pool to the first rinsing pool, the second rinsing pool, the cleaning pool and the elution pool step by step, and the rinsing, cleaning and elution operations are performed respectively to remove non-specifically bound impurities and interferences, and finally a high-purity target molecule solution is obtained in the elution pool;

[0049] Next, the sampling needle 29 is driven down by the second cylinder 10 to absorb the unpurified antibody solution from the elution pool, and then moved to the top of the liquid inlet head 22, and the antibody solution to be purified is injected into the purification cavity 25 of the desalting column 3 and enters the filter head 46. The exchange resin in the filter head 46 is used to remove the salt in the target molecule solution to obtain a target molecule solution with higher purity;

[0050] Then, the third cylinder 20 pushes the pressing plate 21 to apply low pressure to the multiple piston rods 23, so that the target molecule solution is pressed out from the bottom of the desalting column 3 through the piston plate 24 and collected in the collection tube 4, completing the purification and collection of the target molecule;

[0051] Finally, the collection tube 4 is taken out for downstream application or storage, and the ninety-six-well plate 12, the desalting column 3 (filter head 46), and the collection tube 4 are replaced to extract and purify the next batch of samples.

[0052] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0053] The embodiments described above are only descriptions of the preferred methods of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the claims of the present invention.

Claims

1. An extraction and purification instrument based on superparamagnetic beads, characterized in that: include: A box body (1), one side of the box body (1) is rotatably connected to a box door (2), an extraction base and a purification base are respectively arranged on the inner bottom surface of the box body (1), the extraction base is provided with a sample pool, a magnetic bead pool, a first rinsing pool, a second rinsing pool, a cleaning pool and an elution pool, a plurality of desalting columns (3) are arranged on the purification base, the desalting columns are connected to a liquid inlet head (22), a purification component is arranged in the desalting column, a piston is arranged above the purification component, a collection tube (4) is correspondingly arranged below the desalting column (3), and a driving component corresponding to the piston above and below is arranged above the desalting column (3); A motor (5) is fixedly mounted on the inner wall of the box body (1); the output shaft of the motor (5) is fixedly connected to a screw rod (6); the screw rod (6) is transmission-connected to a slider (7); a pair of smooth rods (8) are slidably penetrated on the slider (7); the smooth rods (8) are fixedly mounted on the side wall of the box body (1); a first cylinder (9) and a second cylinder (10) are fixedly mounted on the bottom surface of the slider (7); the output shaft of the first cylinder (9) is provided with a magnetic rod assembly; the output shaft of the second cylinder (10) is provided with a sampling assembly; the first cylinder (9) and the second cylinder (10) are driven by the sliding of the slider (7) to enable the magnetic rod assembly to slide along the extraction base, and the sampling assembly can correspond to the liquid inlet head (22) up and down.

2. The superparamagnetic bead-based extraction and purification instrument according to claim 1, characterized in that: The extraction base comprises a base frame (11), a ninety-six-well plate (12) is placed on the base frame (11), and the sample pool, the magnetic bead pool, the first rinsing pool, the second rinsing pool, the cleaning pool and the elution pool are arranged on the ninety-six-well plate (12).

3. The extraction and purification instrument based on superparamagnetic beads according to claim 1, characterized in that: The purification base comprises a first support plate (13), the first support plate (13) is fixedly connected to the inner wall of the box body (1), a row of first through holes (14) are formed on the first support plate (13), and the desalting columns (3) are inserted into the first through holes (14).

4. The extraction and purification instrument based on superparamagnetic beads according to claim 3, characterized in that: A connecting plate (15) is rotatably connected to one side of the first support plate (13); a row of ring frames (16) are fixedly connected to the connecting plate (15); the collecting tube (4) is plugged into the ring frames (16); an anode magnet (17) is fixedly connected to one end of the connecting plate (15); a cathode magnet (18) is attracted to the anode magnet (17); and the cathode magnet (18) is fixedly connected to the first support plate (13).

5. The extraction and purification instrument based on superparamagnetic beads according to claim 3, characterized in that: The driving assembly comprises a second support plate (19), the second support plate (19) being fixedly connected to the inner wall of the box body (1), a third cylinder (20) being fixedly mounted on the second support plate (19), and an output end of the third cylinder (20) facing the piston and fixedly connected to a pressure plate (21).

6. The superparamagnetic bead-based extraction and purification instrument according to claim 5, characterized in that: The piston comprises a piston rod (23) and a piston plate (24) which are fixedly connected to each other. The piston rod (23) extends upward from the desalting column (3) to correspond to the pressure plate (21). The piston plate (24) is slidably fitted in the desalting column (3). A purification chamber (25) is provided below the piston plate (24). A one-way communication component is provided on the piston plate (24). The one-way communication component is respectively connected to the liquid inlet head (22) and the purification chamber (25).

7. The extraction and purification instrument based on superparamagnetic beads according to claim 1, characterized in that: The magnetic bar assembly comprises a first mounting plate (26), the top surface of the first mounting plate (26) is fixedly connected to the output shaft of the first cylinder (9), and a row of magnetic bars (27) is fixedly mounted on the bottom surface of the first mounting plate (26).

8. The superparamagnetic bead-based extraction and purification instrument according to claim 1, characterized in that: The sampling assembly comprises a second mounting plate (28), the top surface of the second mounting plate (28) is fixedly connected to the output shaft of the second cylinder (10), a row of sampling needles (29) are fixedly mounted on the bottom surface of the second mounting plate (28), the sampling needles (29) are connected to a second air pump (30), and the second air pump (30) is fixedly mounted on the second mounting plate (28).

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