Antifouling cell culture plate
By setting a detachable well cover and rotary opener on the cell culture plate, the problems of cross-contamination and low utilization of the cell culture plate are solved, and multiple uses and efficient utilization of resources are achieved.
Patent Information
- Application Number
- CN202420763958.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-15
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-04-15
AI Technical Summary
Due to cross-contamination and low utilization, existing cell culture plates are mostly used for one-time use, resulting in waste.
An anti-fouling cell culture plate was designed. By setting multiple culture holes on the culture plate, each hole cover can be detached. A groove is provided on the inside of the hole cover to match the internal thread and external thread, and rotation is adopted. The opening driver and transparent material are composed of a hole cover to remove and close, avoid cross-contamination, and improve utilization.
It effectively avoids cross-contamination between culture wells, improves the utilization rate of culture wells on a single culture plate, and allows cell culture plates to be used multiple times, reducing waste.
Smart Images
Figure CN222205265U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of cell culture plates, and particularly relates to an anti-fouling cell culture plate. Background Art
[0002] Cell culture plates are a commonly used cell culture consumables, widely used in the field of biomedicine. Cell culture plates are usually designed in a rectangular shape, consisting of a base and a lid on top. There is a cylindrical space of the same size in each culture plate, namely the cell culture well. When using a cell culture plate, open the upper lid by hand and use tools such as a pipette and tweezers to operate the cells in the culture well. In order to avoid human-caused cell contamination, cells that need to be cultured are usually operated in a clean bench, including unpacking the fully packaged cell culture plates. When sampling and testing of cells and their cultures is required, it may be done in a general laboratory environment. Operation under this condition destroys the original sterile environment in the cell culture plate, resulting in the inability to continue to culture cells in unused cell culture wells. As a result, most of the current cell culture plates are disposable, causing waste.
[0003] Therefore, an anti-fouling cell culture plate that can fully utilize the cell culture wells and reduce the possibility of cross-contamination between cell culture wells is in urgent need of appearance. Utility Model Content
[0004] The utility model provides an anti-fouling cell culture plate, which is used to solve the technical problems of easy cross contamination and low utilization rate of the cell culture plate in the prior art.
[0005] The utility model is realized through the following technical scheme: an anti-fouling cell culture plate, comprising a culture plate, a cover plate and a hole cover, wherein the cover plate is covered on the culture plate, the culture plate is evenly provided with a plurality of culture holes, each of the culture holes is detachably connected with a hole cover, the inner side of the cover plate is provided with a groove for accommodating the hole cover, the number of the grooves is multiple, and the multiple grooves correspond one-to-one to the positions of the culture holes.
[0006] In order to better realize the utility model, further optimization is made in the above structure. The opening of the culture hole is extended with an annular boss, the outer wall of the annular boss is provided with an external thread, the inner wall of the hole cover is provided with an internal thread, and the hole cover is detachably covered on the annular boss through the cooperation of the internal thread and the external thread.
[0007] In order to better realize the utility model, further optimization is made in the above structure, the inner wall of the hole cover is symmetrically provided with two smooth walls along the length direction, and the inner diameter of the smooth wall is the same as the bottom diameter of the inner thread of the hole cover.
[0008] In order to better realize the utility model, further optimization is made in the above structure, which also includes a screwdriver for rotating to open the hole cover, the screwdriver includes two elastic plates connected in a V shape, the free end of the connecting plate is provided with a slot hole, and the outer wall of the hole cover is symmetrically provided with two convex strips matching the slot hole.
[0009] In order to better realize the utility model, further optimization is made in the above structure, and the groove is a square groove.
[0010] In order to better realize the utility model, further optimization is made in the above structure, which also includes a storage box, the storage box includes a box body and a box cover, one side of the box cover is hinged to the box body by a hinge, and the other side is connected to the box body by a buckle, and a convex column is provided on the inner side of the bottom of the box body. The number of the convex columns is multiple, and the multiple convex columns correspond one by one to the positions of the culture holes, and the outer wall of the convex column is provided with an external thread matching the internal thread of the hole cover.
[0011] In order to better realize the utility model, further optimization is made in the above structure, and a plurality of straps are provided on the inner side of the box cover, and the screwdriver is fixed on the inner side of the box cover by the straps.
[0012] In order to better realize the utility model, further optimization is made in the above structure, and the culture plate, cover plate, hole cover and storage box are all structural parts made of transparent materials.
[0013] In order to better implement the utility model, further optimization is made in the above structure, and corresponding digital marks are provided on the grooves and the protruding columns.
[0014] Compared with the prior art, the utility model has the following beneficial effects:
[0015] The antifouling cell culture plate provided by the utility model comprises a culture plate, a cover plate and a hole cover, wherein the cover plate is covered on the culture plate, the culture plate is evenly provided with a plurality of culture holes, each culture hole is detachably connected with a hole cover, the inner side of the cover plate is provided with grooves for accommodating the hole covers, the number of the grooves is multiple, and the multiple grooves correspond to the positions of the culture holes one by one. With this structure, all the culture holes can be closed by setting a plurality of hole covers, during the experiment, only the required culture holes need to be opened, and the other culture holes are still in a state of being closed by the hole covers, thereby avoiding cross contamination between the culture holes, and after the experiment is finished, the culture holes used are closed again without affecting the other culture holes to continue the experiment, thereby improving the utilization rate of the culture holes on a single culture plate, making the utility model more practical. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0017] Figure 1 It is a schematic diagram of the structure of the antifouling cell culture plate in the utility model;
[0018] Figure 2 It is a schematic diagram of the inner side of the cover plate in the utility model;
[0019] Figure 3 It is a structural schematic diagram of the storage box in the utility model;
[0020] Figure 4 It is a structural schematic diagram of the screwdriver in the utility model;
[0021] Figure 5 It is a structural schematic diagram of the hole cover in the utility model.
[0022] In the figure:
[0023] 1-culture plate; 2-cover plate; 3-hole cover; 4-culture hole; 5-groove; 6-annular boss; 7-screwdriver; 8-elastic plate; 9-slot hole; 10-convex strip; 11-box body; 12-box cover; 13-binding strap; 14-convex column; 15-plain wall. DETAILED DESCRIPTION
[0024] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the utility model.
[0025] In the description of the present utility model, it should be noted that, unless otherwise specified, "multiple" means two or more; the orientations or positional relationships indicated by the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present utility model. In addition, the terms "first", "second", "third", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.
[0026] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.
[0027] Embodiment 1:
[0028] In this embodiment, an antifouling cell culture plate 1, such as Figure 1 and Figure 2 As shown, it includes a culture plate 1, a cover plate 2 and a hole cover 3. Specifically, the cover plate 2 is covered on the culture plate 1. The cover plate 2 is breathable. The culture plate 1 is evenly provided with a plurality of culture holes 4. The plurality of culture holes 4 are arranged in a matrix, preferably with six holes, twelve holes or twenty-four holes. Each of the culture holes 4 is detachably connected with a hole cover 3. The inner side of the cover plate 2 is provided with a groove 5 for accommodating the hole cover 3. The number of the grooves 5 is multiple, and the plurality of grooves 5 correspond to the positions of the culture holes 4 one by one.
[0029] By adopting this structure, all the above-mentioned culture holes 4 are closed by setting a plurality of the above-mentioned hole covers 3. During the experiment, only the required culture holes 4 need to be opened, and the other culture holes 4 are still in a closed state by the above-mentioned hole covers 3, thereby avoiding cross contamination between the above-mentioned culture holes 4. After the experiment is over, the above-mentioned culture holes 4 that are used are closed again, which does not affect the continuation of the experiment in other culture holes 4, thereby improving the utilization rate of the culture holes 4 on the single above-mentioned culture plate 1, making the practicality of the utility model more stronger.
[0030] As a specific implementation of this embodiment, Figure 1As shown, an annular boss 6 is extended from the hole mouth of the culture hole 4, the outer wall of the annular boss 6 is provided with an external thread, the inner wall of the hole cover 3 is provided with an internal thread, and the hole cover 3 is detachably covered on the annular boss 6 through the cooperation of the internal thread and the external thread. In this way, the annular boss 6 is higher than the upper plane of the culture plate 1, and the culture hole 4 is closed by the hole cover 3, so that there will be no cross contamination between the culture holes 4.
[0031] In this embodiment, Figure 5 As shown, the inner wall of the hole cover 3 is symmetrically provided with two light walls 15 along the length direction, and the inner diameter of the light wall 15 is the same as the bottom diameter of the internal thread of the hole cover 3. With such arrangement, when the hole cover is screwed with the annular boss 6, the light wall 15 will not be screwed with the external thread of the annular boss 6, and a gap will be formed between the light wall 15 and the external thread at the corresponding position of the annular boss 6, so that it has a certain air permeability, which is beneficial to cell culture.
[0032] In this embodiment, Figure 3 and Figure 4 As shown, it also includes a screwdriver 7 for rotating to open the hole cover 3. The screwdriver 7 includes two elastic plates 8 connected in a V-shape. The free end of the connecting plate is provided with a slot 9. The outer wall of the hole cover 3 is symmetrically provided with two convex strips 10 matching the slots 9. When it is necessary to open the hole cover 3, the slots 9 on the two elastic plates 8 are respectively hung on the two convex strips 10, and then the screwdriver 7 is rotated, thereby driving the hole cover 3 to rotate to open the culture hole 4. As an optimization, the groove 5 is a square groove so that the hole cover 3 and the convex strips 10 thereon can be completely accommodated in the groove 5.
[0033] In this embodiment, Figure 3 As shown, a storage box is also included, and the storage box is used to temporarily store the hole cover 3. The storage box includes a box body 11 and a box cover 12. One side of the box cover 12 is hinged to the box body 11 by a hinge, and the other side is connected to the box body 11 by a buckle. The culture plate 1 and the cover plate 2 are placed in the storage box. As an optimization, a plurality of straps 13 are provided on the inner side of the box cover 12. The plurality of straps 13 are arranged at intervals in a straight line, and the screwdriver 7 is placed on the inner side of the box cover 12 through the straps 13.
[0034] Further, such as Figure 3 As shown, a boss 14 is provided on the inner side of the bottom of the box body 11. There are multiple bosses 14, and the multiple bosses 14 correspond one-to-one to the positions of the culture holes 4. The outer wall of the boss 14 is provided with an external thread matching the internal thread of the hole cover 3, so that the hole cover 3 can be screwed onto the boss 14. During the experiment, the boss 14 is used to temporarily place the hole cover 3.
[0035] In this embodiment, the culture plate 1, cover plate 2, hole cover 3, and storage box are all structural components made of transparent materials with good light transmittance, which facilitates observation of the experimental process.
[0036] As an optimization, the grooves 5 and the bosses 14 are provided with corresponding digital marks to facilitate recording of the experimental process and results.
[0037] Application scenario process of the utility model: when cell culture is performed for the first time, unpack the disposable package in the clean bench and take out the present invention, open the culture plate 1 and the storage box respectively, take out the screwdriver 7 in the storage box, unscrew the corresponding number of the hole covers 3 according to the experimental design requirements, and temporarily fix them at the bottom of the storage box according to the corresponding position sequence, use a pipette to add the cell suspension to the opened culture wells 4, and after completing the rest of the operations, install the hole covers 3 in the storage box back on the culture wells 4. Then cover the culture plate 1 and place it in an incubator in working state for cell culture, and fix the screwdriver 7 in the storage box and store it in a clean bench or other sterile environment. According to the experimental needs, the above-mentioned cover plate 2 or the hole cover 3 can be marked with an oil pen. When cells are needed to perform biomedical experiments, after confirming that the cell status is completed in the clean bench, the hole cover 3 on the above-mentioned culture hole 4 where the cells or suspension are needed is opened and placed in the above-mentioned storage box, and the clean bench can be taken out to perform the corresponding experiment. After the experiment is completed, the above-mentioned culture plate 1 is placed in the clean bench, the waste liquid in the used culture hole 4 is sucked out, and the hole cover 3 temporarily stored is taken out from the above-mentioned storage box and covered, so as to avoid the used culture hole 4 from causing potential contamination to other unused culture holes 4 and the entire sterile environment, and then use the new culture hole 4 for a new round of cell culture.
[0038] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.
Claims
1. An antifouling cell culture plate, characterized in that: The invention comprises a culture plate (1), a cover plate (2) and a hole cover (3), wherein the cover plate (2) covers the culture plate (1), the culture plate (1) is evenly provided with a plurality of culture holes (4), each of the culture holes (4) is detachably connected to a hole cover (3), and a groove (5) for accommodating the hole cover (3) is provided on the inner side of the cover plate (2), and the number of the grooves (5) is plural, and the plurality of grooves (5) correspond to the positions of the culture holes (4) one by one.
2. The antifouling cell culture plate according to claim 1, characterized in that: An annular boss (6) is provided at the opening of the culture hole (4), the outer wall of the annular boss (6) is provided with an external thread, the inner wall of the hole cover (3) is provided with an internal thread, and the hole cover (3) is detachably covered on the annular boss (6) by the cooperation of the internal thread and the external thread.
3. The antifouling cell culture plate according to claim 2, characterized in that: The inner wall of the hole cover (3) is symmetrically provided with two smooth walls (15) along the length direction, and the inner diameter of the smooth wall (15) is the same as the bottom diameter of the internal thread of the hole cover (3).
4. The antifouling cell culture plate according to claim 3, characterized in that: The invention also comprises a screwdriver (7) for rotating and opening the hole cover (3), wherein the screwdriver (7) comprises two elastic plates (8) connected in a V-shape, a slot hole (9) being provided at the free end of the elastic plate, and two convex strips (10) matching the slot holes (9) being symmetrically provided on the outer wall of the hole cover (3).
5. The antifouling cell culture plate according to claim 4, characterized in that: The groove (5) is a square groove.
6. The antifouling cell culture plate according to claim 4, characterized in that: The invention also comprises a storage box, wherein the storage box comprises a box body (11) and a box cover (12), wherein one side of the box cover (12) is hinged to the box body (11) by a hinge, and the other side is connected to the box body (11) by a buckle, and a convex column (14) is provided on the inner side of the bottom of the box body (11), and the number of the convex columns (14) is multiple, and the multiple convex columns (14) correspond to the positions of the culture holes (4) one by one, and the outer wall of the convex column (14) is provided with an external thread matching the internal thread of the hole cover (3).
7. The antifouling cell culture plate according to claim 6, characterized in that: A plurality of binding straps (13) are provided on the inner side of the box cover (12), and the screwdriver (7) is fixed on the inner side of the box cover (12) by means of the binding straps (13).
8. The antifouling cell culture plate according to claim 7, characterized in that: The culture plate (1), cover plate (2), hole cover (3) and storage box are all structural parts made of transparent materials.
9. The antifouling cell culture plate according to claim 6, characterized in that: The grooves (5) and the bosses (14) are both provided with corresponding numerical markings.