Biological culture plate

Abstract

The utility model discloses a biological culture plate, which is characterized in that a pool area is arranged at the periphery of a culture area, so that liquid can be injected into the pool area, the humidity in the culture plate is balanced under the natural evaporation of the liquid in the pool area, the natural evaporation rate in a culture unit is reduced, and the change of the concentration of a culture medium / reaction liquid is reduced. Therefore, the experimental result of long-time culture is closer to the true value.

Description

Technical Field

[0001] The utility model belongs to the field of biological culture equipment, and in particular relates to a biological culture plate. Background Art

[0002] A biological culture plate is a laboratory instrument made mainly of glass and plastic, and is mainly round and square in shape. It is usually used to culture microorganisms, cells, etc. The culture plate is generally composed of a culture base and a culture cover. A number of culture units are arranged on the culture base, so that different groups (different components, concentrations, and amounts of culture media / reaction solutions) are placed in different culture units and cultured separately at the same time to obtain multiple sets of experimental data, and the culture cover is placed on the culture base to cover and protect each culture unit.

[0003] In the existing culture process, the water in the culture unit will naturally evaporate slowly, causing the concentration of the culture medium / reaction solution to change. When the culture time is short, the concentration change caused by the small natural evaporation amount is small, making the error of the experimental result close to the true result. However, when the culture time is long, the concentration change is large due to the increase in natural evaporation, thus affecting the authenticity of the experimental results. Utility Model Content

[0004] In view of the shortcomings of the prior art, the purpose of the utility model is to provide a biological culture plate, which adopts the arrangement of a pool area on the periphery of the culture area so that liquid can be injected into the pool area, and the humidity in the culture plate is balanced under the natural evaporation of the liquid in the pool area, thereby reducing the natural evaporation rate in the culture unit and reducing the change in the concentration of the culture medium / reaction liquid, so that the experimental results of long-term culture are closer to the true value.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: comprising: a culture base, the culture base comprising a bottom plate and a surrounding plate arranged on the periphery of the bottom plate, a culture domain and a pool domain are arranged on the upper side of the bottom plate, the pool domain is for liquid injection, the pool domain is located between the culture domain and the surrounding plate, and a plurality of culture units are arranged in the culture domain; a culture cover, which is used to cover the culture base to seal each culture unit.

[0006] The utility model is further configured as follows: the pool area is arranged in a ring shape on the periphery of the culture area.

[0007] The utility model is further configured as follows: an inner extension portion extending to the top of the pool area is arranged on the upper side of the enclosure.

[0008] The utility model is further configured as follows: the inner extension portion is arranged in a ring shape.

[0009] The utility model is further configured as follows: a separation distance is provided between each of the culture units and adjacent culture units, and a groove area is formed between each of the culture units and at least two adjacent culture units, and the groove area is used for liquid injection.

[0010] The utility model is further configured as follows: a first-level reinforcing rib is provided between each of the separation intervals for connecting the corresponding two culture units, the bottom of at least one of the first-level reinforcing ribs is fixedly connected to the bottom plate; the upper end height of at least one of the first-level reinforcing ribs is lower than the upper end height of each culture unit.

[0011] The utility model is further configured as follows: secondary reinforcing ribs are arranged in the pool area, the secondary reinforcing ribs are respectively fixedly connected to the enclosure and the bottom plate, and the secondary reinforcing ribs are also fixedly connected to the primary reinforcing ribs and / or the culture units.

[0012] The utility model is further configured as follows: the culture units are arranged in a matrix; the bottom plate is located in the pool area and is provided with a first arrangement mark to identify each culture unit; and / or the inner extension is provided with a second arrangement mark to identify each culture unit.

[0013] The utility model is further configured as follows: a spacer ring is respectively arranged under the culture cover corresponding to each culture unit, and each spacer ring is sleeved on the outer periphery of the corresponding culture unit; and / or, a plurality of first spacers are circumferentially arranged under the culture cover, and each first spacer is used to press downward against the upper end of the enclosure to form a gap between the enclosure and the culture cover; and / or, a positioning ring surrounding the outer periphery of the enclosure is arranged under the culture cover, and a plurality of second spacers are circumferentially arranged on the inner periphery of each positioning ring, and each second spacer is used to form a gap between the inner peripheral wall of the positioning ring and the outer peripheral wall of the enclosure.

[0014] The utility model is further configured as follows: the outer periphery of the enclosure is quadrilateral, at least one corner end of the enclosure is provided with an anti-mistake portion, and the culture cover is provided with an alignment portion corresponding to the anti-mistake portion.

[0015] By adopting the above technical scheme, 1. by placing the cultures of different experimental groups in different culture units, and then adding liquid in the pool area, the liquid can be water or a specific culture solution, and finally covering it with a culture cover on the culture base for biological culture; 2. by evaporating the liquid in the pool area, the humidity of the environment in the culture plate is increased, thereby reducing the evaporation rate of the culture medium / reaction solution in the culture unit, reducing the change in the concentration of the culture medium / reaction solution, so that the experimental results of short-term and long-term culture are closer to the true value; 3. a certain distance needs to be set between the culture area and the enclosure to prevent the culture unit from being too close to the enclosure and being affected by the edge of the culture cover, and the pool area is set between the culture area and the enclosure, so that the pool area effectively utilizes the spacing between the culture area and the enclosure, making the structure more compact, and the pool area utilizes the spacing between the culture area and the enclosure to more conveniently pour the liquid, and it is not easy to pour the liquid too close to the culture unit and cause the liquid to enter the culture unit. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 This is an assembly diagram of the first embodiment of the utility model;

[0018] Figure 2 This is an exploded view of the first embodiment of the utility model;

[0019] Figure 3 It is a cross-sectional view of the first embodiment of the utility model;

[0020] Figure 4 It is a parts diagram of the culture base in the first embodiment of the utility model;

[0021] Figure 5 It is a parts diagram of the culture cover in the first embodiment of the utility model;

[0022] Figure 6 It is a top view of the culture base in the first embodiment of the utility model.

[0023] Numbers: 1. Culture base; 11. Bottom plate; 12. Enclosure; 13. Culture area; 14. Pool area; 15. Culture unit; 16. Inner extension; 121. Anti-mistake part; 151. Separation spacing; 152. Groove area; 153. Primary reinforcement rib; 131. Secondary reinforcement rib; 132. First arrangement mark; 2. Culture cover; 21. Spacer ring; 22. First spacer; 23. Positioning ring; 24. Second spacer; 25. Alignment part. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the present invention, and thus to more clearly define the scope of protection claimed by the present invention, the present invention is described in detail below with respect to certain specific embodiments of the present invention. It should be noted that the following are only certain specific implementation methods of the present invention, which are only part of the embodiments of the present invention, wherein the specific and direct description of the relevant structures is only for the convenience of understanding the present invention, and each specific feature does not naturally and directly limit the scope of implementation of the present invention. The conventional selections and substitutions made by those skilled in the art under the guidance of the present invention shall be deemed to be within the scope of protection claimed by the present invention.

[0025] Embodiment 1:

[0026] like Figure 1-Figure 6 As shown, the utility model discloses a biological culture plate, comprising:

[0027] A culture substrate 1, the culture substrate 1 comprises a bottom plate 11 and a surrounding plate 12 integrally formed on the outer periphery of the bottom plate 11, a culture area 13 and a pool area 14 are arranged on the upper side of the bottom plate 11, the pool area 14 is located between the culture area 13 and the surrounding plate 12, a plurality of culture units 15 are arranged in the culture area 13, specifically, the culture unit 15 is a cylindrical tube, and the lower end of the cylindrical tube is integrally formed on the bottom plate 11, and the upper end is open;

[0028] The culture cover 2 is used to cover the culture substrate 1 to seal each culture unit 15 .

[0029] Therefore, 1. by placing the cultures of different experimental groups (including the culture medium / reaction solution with different components, concentrations, dosages, etc.) in different culture units 15, and then adding liquid in the pool area 14, wherein the liquid is preferably a sterile solution, and the liquid can be water or a specific culture solution, and finally covering the culture base 1 with a culture cover 2 for biological culture, 2. by allowing the liquid in the pool area 14 to evaporate naturally to increase the humidity of the environment in the culture plate, thereby reducing the natural evaporation rate of the culture medium / reaction solution in the culture unit, reducing the change in the concentration of the culture medium / reaction solution, so that the culture medium / reaction solution can be cultured in a short time. , the experimental results of long-term cultivation are closer to the true value, 3. A certain distance needs to be set between the culture domain 13 and the enclosure 12 to prevent the culture unit 15 from being too close to the enclosure 12 and being affected by the edge of the culture cover 2, and the pool domain 14 is set between the culture domain 13 and the enclosure 12, so that the pool domain 14 can effectively use the spacing space between the culture domain 13 and the enclosure 12, making the structure more compact, and the pool domain 14 uses the spacing space between the culture domain 13 and the enclosure 12 to pour the liquid more conveniently, and it is not easy to get too close to the culture unit 15 when pouring the liquid, causing the liquid to enter the culture unit 15.

[0030] The culture base 1 and the culture cover 2 are square in shape as a whole.

[0031] Preferably, the pool area 14 in this embodiment is arranged in a ring shape on the periphery of the culture area 13, so that there is liquid on the periphery of the culture area 13, so that the liquid evaporates evenly in the circumference of the culture area 13, making the humidity in the corresponding space of each culture unit 15 more uniform, and finally achieving that each culture unit 15 is affected by evaporation to be equivalent, so as to prevent deviations in experimental results caused by different evaporation rates.

[0032] In other embodiments, the pool area 14 is only arranged on one side, two sides or three sides of the culture area 13 .

[0033] In addition, the upper side of the enclosure 12 in this embodiment is provided with an inner extension portion 16 extending to above the pool area 14, so that the inner extension portion 16 can provide a certain width for writing marks to assist in experimental recording.

[0034] Preferably, the inner extension 16 in this embodiment is arranged in an annular shape, so that the outer periphery of the annular inner extension 16 is integrally formed circumferentially with the upper end of the enclosure 12, so that the structural strength is better and the markable space is larger.

[0035] In addition, a separation distance 151 is set between each of the culture units 15 and the adjacent culture units 15 in this embodiment, and a groove area 152 is formed between each of the culture units 15 and the three adjacent culture units 15. Therefore, 1. liquid can be added in each groove area 152, so that the liquid in the pool area 14 evaporates at the periphery of the culture area 13 and diffuses from the periphery into the culture area 13, and the liquid in each groove area 152 evaporates in the culture area 13 and diffuses toward various parts of the culture area 13, so that the interaction between the two further increases the uniformity of humidity in various parts of the culture area 13; 2. the culture units 15 are separated from each other under the action of the separation distance 151 to reduce the mutual interference.

[0036] The culture units 15 are arranged in a matrix, and because the outer periphery of the culture units 15 is cylindrical, each groove area 152 is similar to a diamond structure.

[0037] In addition, a first-level reinforcing rib 153 is arranged between each of the separation intervals 151 in this embodiment, and the horizontal ends of each first-level reinforcing rib 153 are respectively integrally formed on the outer peripheral wall of the corresponding culture unit 15 to connect the corresponding two culture units 15, and the bottom of each first-level reinforcing rib 153 is fixedly connected to the base plate 11.

[0038] Therefore, the adjacent culture units 15 and the bottom plate 11 can be fixed by the primary reinforcing ribs 153 to improve the strength of the culture units 15 .

[0039] Preferably, the upper end height of each of the first-level reinforcing ribs 153 in this embodiment is lower than the upper end height of each culture unit 15. Therefore, 1. The first-level reinforcing ribs 153 are too low to connect the upper ends of adjacent culture units 15, so as to prevent the culture in the culture unit 15 from entering the adjacent culture unit 15 through the first-level reinforcing ribs 153 and causing interference; 2. The first-level reinforcing ribs 153 are too low to allow continuous injection of liquid into the pool area 14 without passing through the first-level reinforcing ribs 153 and entering the trough area 152, so that only the pool area 14 needs to be injected with liquid, and the operation is more convenient.

[0040] In other embodiments, the primary reinforcing ribs 153 are not provided, so that the liquid in the pool area 14 can be continuously injected directly into the groove area 152 to fill the groove area 152 with liquid.

[0041] Preferably, a plurality of secondary reinforcement ribs 131 are provided in the pool area 14 in this embodiment, and the secondary reinforcement ribs 131 are respectively fixedly connected to the enclosure 12 and the bottom plate 11, and the secondary reinforcement ribs 131 are also fixedly connected to the primary reinforcement ribs 153, so that the combination of the culture unit 15 and the primary reinforcement ribs 153 is connected to the enclosure 12 through the secondary reinforcement ribs 131, thereby further increasing the strength of the culture plate.

[0042] In other embodiments, the secondary reinforcing rib 131 is not connected to the primary reinforcing rib 153 , but is connected to the culture unit 15 .

[0043] Among them, the bottom plate 11 in this embodiment is located in the pool area 14 and is provided with two horizontal and vertical rows of first arrangement marks 132, namely 1-12 horizontally to mark 12 columns and AH vertically to mark 8 columns, so as to assign a number to each culture unit 15.

[0044] Among them, a spacer ring 21 is respectively arranged under the culture cover 2 corresponding to each culture unit 15 in this embodiment, and each spacer ring 21 is sleeved on the outer periphery of the corresponding culture unit 15 so that the outer periphery of the corresponding culture unit 15 is covered by the spacer ring 21 and further separated from other culture units 15.

[0045] Among them, the culture cover 2 in this embodiment is provided with a plurality of first spacers 22 along the circumferential direction below, and when the culture cover 2 is placed on the culture base 1, each first spacer 22 is downwardly pressed against the upper end of the enclosing plate 12 to form a gap between the enclosing plate 12 and the culture cover 2. Under the action of the first spacers 22, the gap between the enclosing plate 12 and the culture cover 2 allows the outside air to enter the culture plate, so that an aerobic environment is formed in the culture unit 15 for culture, and each circumferential first spacer 22 lifts the culture cover 2 horizontally, so that air can enter the culture cover 2 evenly in the circumferential direction, so that the air environment in each culture unit 15 is equivalent.

[0046] Among them, a positioning ring 23 surrounding the outer circumference of the enclosure 12 is arranged below the culture cover 2 in this embodiment, and a plurality of second spacers 24 are arranged along the circumferential direction on the inner circumference of each positioning ring 23, and each second spacer 24 is used to form a gap between the inner circumferential wall of the positioning ring 23 and the outer circumferential wall of the enclosure 12. Therefore, 1. The culture cover 2 is limitedly matched on the culture base 1 by the positioning ring 23 being sleeved on the outer circumference of the enclosure 12, and 2. Under the action of the second spacers 24, the gap between the enclosure 12 and the positioning ring 23 allows the outside air to enter the culture plate so that an aerobic environment is formed in the culture unit 15 for culture, and each circumferential second spacer 24 ensures that each part of the positioning ring 23 cannot fit on the corresponding part of the enclosure 12, so that air can enter the culture cover 2 evenly in the circumferential direction, so that the air environment in each culture unit 15 is equivalent.

[0047] Preferably, the thickness of each second spacer 24 in this embodiment decreases downward, so that it is inclined and has a certain guiding effect, so that the culture cover 2 and the culture base 1 can be matched more smoothly.

[0048] The first partitions 22 are provided at the four corner ends of the culture cover 2 , and the second partitions 24 are corresponding extensions of the first partitions 22 .

[0049] In addition, one or two adjacent corner ends of the enclosure 12 in this embodiment are provided with an anti-mistake portion 121, and correspondingly, the culture cover 2 is provided with an alignment portion 25 corresponding to the anti-mistake portion 121, so that the culture cover 2 can be matched with the culture base 1 as much as possible when the anti-mistake portion 121 and the alignment portion 25 are aligned, so as to prevent the culture units 15 from interfering with each other when they are matched with different positions of the culture cover 2 due to repeated removal of the culture cover 2.

[0050] Embodiment 2:

[0051] A biological culture plate, whose main structure is the same as that of the first embodiment, except that the overall structure of the culture base 1 and the culture cover 2 is more circular.

[0052] Embodiment three:

[0053] A biological culture plate, the main structure of which is the same as that of the first embodiment, except that the inner extension 16 is not provided; or,

[0054] The inner extension 16 is provided only at a part of the enclosure 12 .

[0055] Embodiment 4:

[0056] A biological culture plate, whose main structure is the same as that of the first embodiment, except that the upper ends of some or all of the primary reinforcing ribs 153 are flush with or higher than the upper ends of the culture units 15, so that the liquid in the pool area 14 cannot flow into the trough area 152, and the liquid in the trough area 152 needs to be added one by one.

[0057] Embodiment five:

[0058] A biological culture plate, whose main structure is the same as that of the first embodiment, except that the first arrangement mark 132 is not set on the bottom plate 11, but the second arrangement mark is set on the inner extension 16. The principle is the same as that of the first arrangement mark 132, but it is more convenient to observe when it is set on the inner extension 16.

[0059] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A biological culture plate, characterized in that: include: A culture substrate (1), the culture substrate (1) comprising a bottom plate (11) and a surrounding plate (12) arranged on the periphery of the bottom plate (11), a culture area (13) and a pool area (14) being arranged on the upper side of the bottom plate (11), the pool area (14) being used for liquid injection, the pool area (14) being located between the culture area (13) and the surrounding plate (12), and a plurality of culture units (15) being arranged in the culture area (13); A culture cover (2) used to cover the culture base (1) to seal each culture unit (15); A separation distance (151) is provided between each of the culture units (15) and an adjacent culture unit (15), and a groove area (152) is formed between each of the culture units (15) and at least two adjacent culture units (15), and the groove area (152) is used for liquid injection.

2. The biological culture plate according to claim 1, characterized in that: The pool area (14) is arranged in a ring shape on the periphery of the culture area (13).

3. The biological culture plate according to claim 2, characterized in that: An inner extension portion (16) extending to above the pool area (14) is provided on the upper side of the enclosure plate (12).

4. The biological culture plate according to claim 3, characterized in that: The inner extension portion (16) is arranged in a ring shape.

5. The biological growth plate according to claim 1, characterized in that: A primary reinforcing rib (153) for connecting the corresponding two culture units (15) is provided between each of the separation intervals (151), and the bottom of at least one of the primary reinforcing ribs (153) is fixedly connected to the bottom plate (11); The upper end height of at least one of the primary reinforcing ribs (153) is lower than the upper end height of each culture unit (15).

6. The biological growth plate according to claim 5, characterized in that: The pool area (14) is provided with secondary reinforcing ribs (131), and the secondary reinforcing ribs (131) are respectively fixedly connected to the enclosure (12) and the bottom plate (11), and the secondary reinforcing ribs (131) are also fixedly connected to the primary reinforcing ribs (153) and / or the culture unit (15).

7. The biological growth plate according to claim 3 or 4, characterized in that: The culture units (15) are arranged in a matrix; The bottom plate (11) is located in the pool area (14) and is provided with a first arrangement mark (132) for marking each culture unit (15); and / or, The inner extension (16) is provided with a second arrangement mark for identifying each culture unit (15).

8. The biological growth plate according to claim 1, characterized in that: A spacer ring (21) is provided below the culture cover (2) corresponding to each culture unit (15), and each spacer ring (21) is sleeved on the outer circumference of the corresponding culture unit (15); and / or, a plurality of first spacers (22) are provided below the culture cover (2) along the circumferential direction, and each first spacer (22) is used to abut against the upper end of the enclosing plate (12) downward to form a gap between the enclosing plate (12) and the culture cover (2); and / or, A positioning ring (23) is arranged below the culture cover (2) and surrounds the outer circumference of the enclosure (12). A plurality of second spacers (24) are arranged along the circumferential direction on the inner circumference of each positioning ring (23). Each second spacer (24) is used to form a gap between the inner circumferential wall of the positioning ring (23) and the outer circumferential wall of the enclosure (12).

9. The biological growth plate according to claim 8, characterized in that: The outer periphery of the enclosure (12) is in the shape of a quadrilateral, at least one corner end of the enclosure (12) is provided with an anti-mistake portion (121), and the culture cover (2) is provided with an alignment portion (25) corresponding to the anti-mistake portion (121).

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