Sample tube fixing device for ultrasonically crushing cells

Abstract

The utility model provides a sample tube fixing device for ultrasonication of cells, which relates to the technical field of ultrasonication of cells and comprises an ice water container, a metal module placed at the bottom of the inner wall of the ice water container, a preset hole formed in the top of the metal module, and a sample tube body placed at the bottom of the inner wall of the preset hole. An ultrasonic probe is placed at the bottom of the inner wall of the sample tube body, and two side face small holes are formed in the outer surface wall of the metal module. According to the utility model, the metal module can be immersed in an ice-water mixture and sinks at the bottom of the ice-water container under the action of the material of the metal module, then the sample tube is placed in the preset hole, and the first handle and the second handle are rotated to respectively drive the first screw rod and the second screw rod to rotate towards the inside of the preset hole; the sample tube is fixedly limited in the preset hole, and the fixing effect is better than that of a researcher using a test tube rack or an adhesive tape, so that the use effect of the sample (tube) fixing device for ultrasonically crushing the cells is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of ultrasonic cell disruption, in particular to a sample tube fixing device used for ultrasonic cell disruption. Background Art

[0002] Ultrasonic cell disruption is a process that utilizes the mechanical effect of ultrasound to generate high-frequency vibrations and shear forces to disrupt cells. By placing cells in an ultrasonic field, the vibrations of ultrasound will cause pressure changes and shear forces inside and outside the cells, thereby destroying the cell structure and membrane, causing the cells to release their internal components. Nowadays, ultrasonic cell disruption is a common method of cell disruption. Ultrasonic cell disruption is mainly used for the following purposes, such as cell disruption and release of cell contents, DNA / RNA extraction and cell lysis and extract processing. It can disrupt cells, release cell components, extract biological samples, etc. It is widely used in biomedical research, bioengineering, biopharmaceuticals and other fields.

[0003] Existing, such as a test tube fixing device for sample extraction in Chinese patent number CN214439288U, comprises a supporting base, a rotating support member, an up and down driving member, a sliding adjustment member and a clamping member, wherein the supporting base comprises a bottom plate, and supporting legs are arranged at four corners of the lower surface of the bottom plate, a rotating support member is arranged at the center of the upper surface of the supporting base, a sliding adjustment member is slidably connected to the rotating support member, a clamping member is arranged at one end of the sliding adjustment member, and an up and down driving member for driving the sliding adjustment member to move up and down is arranged on the rotating support member, the test tube can be clamped by the test tube clamp, and the inclination angle of the test tube can be adjusted by rotating the turntable, making the adjustment more convenient. The test tube fixing device for sample extraction has a simple structure, which not only makes the test tube adjustment more comprehensive, but also makes it more convenient to use, providing convenience for people.

[0004] However, during the ultrasonic fragmentation process, in order to prevent the sample from heating up, the researchers need to place the sample tube in an ice box, but it is difficult to keep the sample tube stable because the ice will melt and the sample tube will move, thus touching the ultrasonic probe and affecting the experimental results. As the ice melts, the sample tube will float, so the ice must be replaced repeatedly, which increases the researchers' working time. Secondly, the contact surface between the ice and the sample tube will move, affecting the cooling effect of the sample tube, thus requiring a sample tube fixing device for ultrasonic fragmentation of cells. Utility Model Content

[0005] The utility model aims to solve the problem that when the existing equipment is in use, ice will melt, the sample tube will move, and then touch the ultrasonic probe, thus affecting the experimental results, and proposes a sample tube fixing device for ultrasonic cell disruption.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a sample tube fixing device for ultrasonically disrupting cells, comprising an ice water container, a metal module is placed at the bottom of the inner wall of the ice water container, a preset hole is opened on the top of the metal module, a sample tube body is placed at the bottom of the inner wall of the preset hole, an ultrasonic probe is placed at the bottom of the inner wall of the sample tube body, and two side holes are opened on the outer wall of the metal module, wherein the inner wall of one of the side holes is threadedly connected to a first screw, and one side of the outer wall of the first screw contacts the outer wall of the sample tube body, and the inner wall of the other side hole is threadedly connected to a second screw, and one side of the outer wall of the second screw contacts the outer wall of the sample tube body.

[0007] Preferably, a first handle is fixedly mounted on one side of the outer wall of the first screw rod.

[0008] Preferably, a second handle is fixedly mounted on one side of the outer wall of the second screw rod.

[0009] Preferably, the inner walls of the two side holes are both connected to the interior of the preset hole.

[0010] Compared with the prior art, the advantages and positive effects of the utility model are:

[0011] 1. In the utility model, the metal module can be immersed in the ice-water mixture and sink to the bottom of the ice-water container through the effect of its own material. Under the effect of its own gravity, it will not move inside, so that the sample tube will not move as the ice melts, and will not touch the ultrasonic probe, thereby improving the reliability of the experimental results and quickly transferring the heat in the sample tube to the ice-water mixture. Secondly, the sample tube is placed in the preset hole, and the first handle and the second handle are rotated to respectively drive the first screw and the second screw to rotate inside the preset hole, and the sample tube is fixed and limited in the preset hole. The fixing effect is better than that of the researchers using a test tube rack or tape. Secondly, because the sample tube is limited in the metal module, it will not float inside as the ice melts, and there is no need to repeatedly replace the ice, which reduces the labor time of the researchers, thereby improving the use effect of the sample tube fixing device for ultrasonic cell disruption. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 A stereoscopic diagram of a sample tube fixing device for ultrasonic cell disruption proposed by the utility model;

[0013] Figure 2 The utility model provides a disassembled diagram of a sample tube fixing device for ultrasonic cell disruption;

[0014] Figure 3A partial exploded view of a sample tube fixing device for ultrasonic cell disruption proposed by the utility model;

[0015] Figure 4 The utility model provides a partial front view of a sample tube fixing device for ultrasonically disrupting cells.

[0016] Legend:

[0017] 1. Ice water container; 2. Metal module; 3. Preset hole; 4. Sample tube body; 5. Ultrasonic probe; 6. Side hole; 7. First screw; 8. Second screw; 9. First handle; 10. Second handle. DETAILED DESCRIPTION

[0018] In order to more clearly understand the above-mentioned purposes, features and advantages of the utility model, the utility model is further described below in conjunction with the drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0019] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure.

[0020] Embodiment 1, as Figure 1-Figure 4 As shown, the utility model provides a sample tube fixing device for ultrasonic cell disruption, comprising an ice water container 1, a metal module 2 is placed at the bottom of the inner wall of the ice water container 1, a preset hole 3 is opened on the top of the metal module 2, a sample tube body 4 is placed at the bottom of the inner wall of the preset hole 3, an ultrasonic probe 5 is placed at the bottom of the inner wall of the sample tube body 4, and two side holes 6 are opened on the outer wall of the metal module 2, one of the side holes 6 is threadedly connected to the inner wall of a first screw rod 7, and one side of the outer wall of the first screw rod 7 contacts the outer wall of the sample tube body 4, the other side hole 6 is threadedly connected to the inner wall of a second screw rod 8, and one side of the outer wall of the second screw rod 8 contacts the outer wall of the sample tube body 4, a first handle 9 is fixedly installed on one side of the outer wall of the first screw 7, and a second handle 10 is fixedly installed on one side of the outer wall of the second screw 8, and the inner walls of the two side holes 6 are both connected to the inside of the preset hole 3.

[0021] The effect achieved by the entire embodiment 1 is that the metal module 2 is a module made of metal material, such as iron, copper and stainless steel, which can be immersed in water or ice water and sink to the bottom of the ice water container 1. Through its own gravity, it will not easily move in the ice-water mixture in the ice water container, so that the sample tube will not move as the ice melts and will not touch the ultrasonic probe 5, thereby improving the reliability of the experimental results. The heat generated during the ultrasonic cell disruption process can be quickly transferred to the ice-water mixture along the sample tube, and it has a fast heat transfer performance. Then, a preset hole 3 is opened on the metal module 2, and sample tubes of different specifications, such as a 50ml centrifuge tube, a 15ml centrifuge tube and a 1.5ml EP tube, etc., can be placed in the preset hole 3. Secondly, side holes 6 are opened on both sides of the metal module 2, and the inner surface walls of the two side holes 6 are connected to the inside of the preset hole 3, and then the two side holes are opened. The inside of the hole 6 is respectively threadedly connected with a first screw rod 7 and a second screw rod 8, and a first handle 9 is installed on one side of the outer wall of the first screw rod 7, and a second handle 10 is installed on one side of the outer wall of the second screw rod 8. The first handle 9 and the second handle 10 are rotated at the same time to make the first screw rod 7 and the second screw rod 8 move toward the middle in the two side holes 6, and the sample tube body 4 is limited and fixed in the preset hole 3, so that sample tubes of different specifications can be fixed and limited in the preset hole 3, which is better than the fixing effect of researchers using test tube racks or tapes. Secondly, since the sample tube is limited in the metal module 2, it will not float inside as the ice melts, and there is no need to repeatedly replace the ice, which reduces the working time of the researchers. Especially in the ice-water mixture, while achieving the fixation of the sample tube, the cooling effect of the sample tube in the ice-water mixture is also improved, thereby improving the use effect of the sample tube fixing device for ultrasonic cell disruption.

[0022] Working principle: First, place the sample tube in the preset hole 3 opened on the metal module 2, and then rotate the first handle 9 and the second handle 10 to respectively drive the first screw 7 and the second screw 8 to rotate toward the middle, so that the sample tube is limited and fixed in the preset hole 3, and then the metal mold 2 with the sample tube limited is placed in the ice water container 1 filled with ice-water mixture, and the weight and rapid heat transfer performance of the metal module 2 are fully utilized to stably place the metal module 2 in the ice-water mixture, so that the sample tube will not move as the ice melts, and will not touch the ultrasonic probe 5, thereby improving the reliability of the experimental results, and at the same time, the heat generated during the ultrasonic cell disruption process can be quickly transferred along the sample tube to the ice water, thereby improving the cooling effect of the sample tube, and secondly, because the sample tube is limited in the metal module 2, it will not float inside as the ice melts, and there is no need to repeatedly replace the ice, which reduces the labor time of researchers, thereby improving the use effect of the sample tube fixing device for ultrasonic cell disruption.

[0023] The above are only preferred embodiments of the present invention, and are not intended to limit the present invention in other forms. Any technician familiar with the profession may use the technical contents disclosed above to change or modify them into equivalent embodiments with equivalent changes and apply them to other fields. However, any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A sample tube fixing device for ultrasonic cell disruption, comprising an ice water container (1), characterized in that: A metal module (2) is placed at the bottom of the inner wall of the ice water container (1), a preset hole (3) is provided at the top of the metal module (2), a sample tube body (4) is placed at the bottom of the inner wall of the preset hole (3), an ultrasonic probe (5) is placed at the bottom of the inner wall of the sample tube body (4), and two side holes (6) are provided on the outer wall of the metal module (2), one of the side holes (6) is threadedly connected to a first screw (7) on the inner wall, and one side of the outer wall of the first screw (7) is in contact with the outer wall of the sample tube body (4), and the other side hole (6) is threadedly connected to a second screw (8) on the inner wall, and one side of the outer wall of the second screw (8) is in contact with the outer wall of the sample tube body (4).

2. A sample tube fixing device for ultrasonic cell disruption according to claim 1, characterized in that: A first handle (9) is fixedly mounted on one side of the outer wall of the first screw rod (7).

3. A sample tube fixing device for ultrasonic cell disruption according to claim 2, characterized in that: A second handle (10) is fixedly mounted on one side of the outer wall of the second screw rod (8).

4. The sample tube fixing device for ultrasonic cell disruption according to claim 3, characterized in that: The inner walls of the two side small holes (6) are both connected to the interior of the preset hole (3).

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